BR112017020474A2 - levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol - Google Patents

levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol

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Publication number
BR112017020474A2
BR112017020474A2 BR112017020474A BR112017020474A BR112017020474A2 BR 112017020474 A2 BR112017020474 A2 BR 112017020474A2 BR 112017020474 A BR112017020474 A BR 112017020474A BR 112017020474 A BR112017020474 A BR 112017020474A BR 112017020474 A2 BR112017020474 A2 BR 112017020474A2
Authority
BR
Brazil
Prior art keywords
fermentation
nucleic acid
modified yeast
vector
yeast
Prior art date
Application number
BR112017020474A
Other languages
English (en)
Inventor
Vas Amit
Negrete-Raymond Ana
K Miller Christopher
Veldhouse Jon
Original Assignee
Cargill Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cargill Inc filed Critical Cargill Inc
Publication of BR112017020474A2 publication Critical patent/BR112017020474A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção se refere a uma levedura geneticamente modificada com uma glicoamilase heteróloga e a métodos de fermentação. as leveduras modificadas podem ter múltiplas sequências de ácidos nucleicos exógenas, com cada uma tendo uma sequência diferente, mas que codificam a mesma proteína de glicoamilase ou uma similar que é heteróloga à levedura. as leveduras modificadas apresentam perfis desejáveis de produção de bioprodutos durante um processo de fermentação. um meio de fermentação com um material amiláceo pode ser fermentado com a levedura modificada para fornecer títulos elevados de etanol, títulos baixos de glicerol ou ambos.
BR112017020474A 2015-03-27 2016-03-25 levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol BR112017020474A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562139312P 2015-03-27 2015-03-27
PCT/US2016/024249 WO2016160584A1 (en) 2015-03-27 2016-03-25 Glucoamylase-modified yeast strains and methods for bioproduct production

Publications (1)

Publication Number Publication Date
BR112017020474A2 true BR112017020474A2 (pt) 2018-07-03

Family

ID=55808840

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017020474A BR112017020474A2 (pt) 2015-03-27 2016-03-25 levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol

Country Status (6)

Country Link
US (2) US10344288B2 (pt)
EP (1) EP3274461A1 (pt)
CN (1) CN107429220A (pt)
BR (1) BR112017020474A2 (pt)
CA (1) CA2979410A1 (pt)
WO (1) WO2016160584A1 (pt)

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Also Published As

Publication number Publication date
US20180094269A1 (en) 2018-04-05
US10344288B2 (en) 2019-07-09
CN107429220A (zh) 2017-12-01
EP3274461A1 (en) 2018-01-31
US20190376071A1 (en) 2019-12-12
WO2016160584A1 (en) 2016-10-06
CA2979410A1 (en) 2016-10-06

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