BR112016027290A2 - métodos para a produção de ramnolipídeos". - Google Patents
métodos para a produção de ramnolipídeos".Info
- Publication number
- BR112016027290A2 BR112016027290A2 BR112016027290A BR112016027290A BR112016027290A2 BR 112016027290 A2 BR112016027290 A2 BR 112016027290A2 BR 112016027290 A BR112016027290 A BR 112016027290A BR 112016027290 A BR112016027290 A BR 112016027290A BR 112016027290 A2 BR112016027290 A2 BR 112016027290A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- enzyme
- carbon source
- ramnolipid
- ramnosyl
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01159—Flavonol-3-O-glucoside L-rhamnosyltransferase (2.4.1.159)
Abstract
a presente invenção diz respeito a um método para a preparação de pelo menos um ramnolipídeo, o qual compreende: - fazer contactar uma célula recombinante com um meio que contém uma fonte de carbono e - desenvolver em cultura a célula sob condições adequadas para a preparação do ramnolipídeo a partir da fonte de carbono pela célula, em que a célula recombinante foi geneticamente modificada de um modo tal que, em comparação com o tipo selvagem da célula, a célula tem uma atividade aumentada de pelo menos uma das enzimas e1, e2 e e3, em que a enzima e1 é uma a/ß hidrolase, a enzima e2 é uma ramnosil-transferase i e a enzima e3 é uma ramnosil-transferase ii e em que a fonte de carbono é uma molécula c4.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14169799.5A EP2949214A1 (en) | 2014-05-26 | 2014-05-26 | Methods of producing rhamnolipids |
| EP14169799.5 | 2014-05-26 | ||
| PCT/EP2015/059044 WO2015180907A1 (en) | 2014-05-26 | 2015-04-27 | Methods of producing rhamnolipids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112016027290A2 true BR112016027290A2 (pt) | 2018-01-30 |
| BR112016027290B1 BR112016027290B1 (pt) | 2022-07-12 |
Family
ID=50774715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112016027290-0A BR112016027290B1 (pt) | 2014-05-26 | 2015-04-27 | Métodos e sistemas para a detecção de microrganismos |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10174353B2 (pt) |
| EP (2) | EP2949214A1 (pt) |
| JP (1) | JP6742918B2 (pt) |
| CN (1) | CN106414758B (pt) |
| BR (1) | BR112016027290B1 (pt) |
| CA (1) | CA2948696C (pt) |
| RU (1) | RU2694392C9 (pt) |
| WO (1) | WO2015180907A1 (pt) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010032484A1 (de) * | 2010-07-28 | 2012-02-02 | Evonik Goldschmidt Gmbh | Zellen und Verfahren zur Herstellung von Rhamnolipiden |
| EP2949214A1 (en) | 2014-05-26 | 2015-12-02 | Evonik Degussa GmbH | Methods of producing rhamnolipids |
| EP3336180A1 (en) * | 2016-07-04 | 2018-06-20 | Evonik Degussa GmbH | Mutant alkb gene |
| BR112019008321A2 (pt) | 2016-10-24 | 2020-01-28 | Evonik Degussa Gmbh | célula e método de produção de ramnolipídeo que tem atividade de glicose desidrogenase reduzida e seu uso |
| CN107557324B (zh) * | 2017-10-26 | 2019-09-03 | 南京工业大学 | 一株铜绿假单胞菌及其应用 |
| BR112020015687A2 (pt) | 2018-02-09 | 2020-12-08 | Evonik Operations Gmbh | Célula microbiana para produzir pelo menos um lipídeo, método e uso da mesma |
| CN108753810B (zh) * | 2018-05-22 | 2021-06-18 | 昆明理工大学 | 一种转录调节蛋白基因orf2的用途 |
| CN108707562B (zh) * | 2018-05-24 | 2022-05-10 | 江苏世邦生物工程科技有限公司 | 用于修复三氮污染土壤的微生物制剂及其制备方法和应用 |
| CN112980911B (zh) * | 2019-12-18 | 2022-09-20 | 万华化学集团股份有限公司 | 一种低残油发酵生产鼠李糖脂的方法 |
| BR112022018009A2 (pt) | 2020-03-11 | 2022-11-08 | Evonik Operations Gmbh | Composição, método para preparar uma formulação e uso de trietil citrato |
| US11582924B2 (en) | 2020-05-21 | 2023-02-21 | Aquatrols Corporation Of America | Growing media composition |
| CN114231545B (zh) * | 2020-09-09 | 2023-06-20 | 中国中医科学院中药研究所 | 一种鼠李糖转移酶基因、制备方法及其表达和应用 |
| CN114438000B (zh) * | 2020-11-05 | 2024-02-27 | 万华化学(四川)有限公司 | 一株铜绿假单胞菌及其构建方法与应用 |
| US20240117275A1 (en) | 2021-01-29 | 2024-04-11 | Danisco Us Inc. | Compositions for cleaning and methods related thereto |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US601494A (en) | 1898-03-29 | Spar for vessels | ||
| NL301993A (pt) | 1962-12-18 | |||
| US4601893A (en) | 1984-02-08 | 1986-07-22 | Pfizer Inc. | Laminate device for controlled and prolonged release of substances to an ambient environment and method of use |
| DE4027453A1 (de) | 1990-08-30 | 1992-03-05 | Degussa | Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren |
| DE4440118C1 (de) | 1994-11-11 | 1995-11-09 | Forschungszentrum Juelich Gmbh | Die Genexpression in coryneformen Bakterien regulierende DNA |
| JP4327909B2 (ja) | 1996-11-13 | 2009-09-09 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | 組み換え生物による１，３―プロパンジオールの生産方法 |
| US6238896B1 (en) | 1997-02-20 | 2001-05-29 | Mitsubishi Rayon Co., Ltd. | Process for producing malonic acid derivatives |
| JPH10229891A (ja) | 1997-02-20 | 1998-09-02 | Mitsubishi Rayon Co Ltd | マロン酸誘導体の製造法 |
| DE10031999A1 (de) | 1999-09-09 | 2001-04-19 | Degussa | Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien |
| DE102007031689A1 (de) | 2007-07-06 | 2009-01-08 | Evonik Goldschmidt Gmbh | Enzympräparate |
| DE102007060705A1 (de) | 2007-12-17 | 2009-06-18 | Evonik Degussa Gmbh | ω-Aminocarbonsäuren oder ihre Lactame, herstellende, rekombinante Zellen |
| DE102008002410A1 (de) | 2008-06-13 | 2009-12-17 | Evonik Goldschmidt Gmbh | Enzymatische Synthese von Sphingolipiden |
| DE102008002409A1 (de) | 2008-06-13 | 2009-12-17 | Evonik Goldschmidt Gmbh | Enzymatische Synthese von Sphingolipiden |
| DE102008041754A1 (de) | 2008-09-02 | 2010-03-04 | Evonik Goldschmidt Gmbh | Enzympräparate |
| DE102009045077A1 (de) | 2009-09-29 | 2011-03-31 | Evonik Goldschmidt Gmbh | Verwendung von Sophorolipiden und deren Derivaten in Kombination mit Pestiziden als Adjuvant/Additiv für den Pflanzenschutz und den industriellen non-crop Bereich |
| DE102009046623A1 (de) | 2009-11-11 | 2011-05-12 | Evonik Röhm Gmbh | Verwendung eines zu einem MeaB-Protein homologen Proteins zur Erhöhung der enzymatischen Aktivität einer 3-Hydroxycarbonsäure-CoA-Mutase |
| DE102010014680A1 (de) | 2009-11-18 | 2011-08-18 | Evonik Degussa GmbH, 45128 | Zellen, Nukleinsäuren, Enzyme und deren Verwendung sowie Verfahren zur Herstellung von Sophorolipiden |
| DE102010029973A1 (de) | 2010-06-11 | 2011-12-15 | Evonik Degussa Gmbh | Mikrobiologische Herstellung von C4-Körpern aus Saccharose und Kohlendioxid |
| DE102010032484A1 (de) * | 2010-07-28 | 2012-02-02 | Evonik Goldschmidt Gmbh | Zellen und Verfahren zur Herstellung von Rhamnolipiden |
| EP2573172A1 (en) | 2011-09-21 | 2013-03-27 | Heinrich-Heine-Universität Düsseldorf | Means and methods for rhamnolipid production |
| DE102011090030A1 (de) | 2011-12-28 | 2013-07-04 | Evonik Industries Ag | Wässrige Haar- und Hautreinigungszusammensetzungen, enthaltend Biotenside |
| DE102012201360A1 (de) | 2012-01-31 | 2013-08-01 | Evonik Industries Ag | Zellen und Verfahren zur Herstellung von Rhamnolipiden |
| DE102012007491A1 (de) | 2012-04-11 | 2013-10-17 | Evonik Industries Ag | Neue Enzyme |
| DE102012212085A1 (de) | 2012-07-11 | 2014-01-16 | Evonik Industries Ag | Lipase stabiler Verdicker |
| DE102012221519A1 (de) | 2012-11-26 | 2014-05-28 | Evonik Industries Ag | Verfahren zur Isolierung von Rhamnolipiden |
| EP2746397A1 (de) | 2012-12-21 | 2014-06-25 | Evonik Industries AG | Herstellung von Omega-Aminofettsäuren |
| DE102013205756A1 (de) | 2013-04-02 | 2014-10-02 | Evonik Industries Ag | Mischungszusammensetzung enthaltend Rhamnolipide |
| DE102013205755A1 (de) | 2013-04-02 | 2014-10-02 | Evonik Industries Ag | Waschmittelformulierung für Textilien enthaltend Rhamnolipide mit einem überwiegenden Gehalt an di-Rhamnolipiden |
| DE102013206314A1 (de) | 2013-04-10 | 2014-10-16 | Evonik Industries Ag | Kosmetische Formulierung enthaltend Copolymer sowie Sulfosuccinat und/oder Biotensid |
| DE102014200497A1 (de) | 2014-01-14 | 2015-07-16 | Evonik Industries Ag | Enzymatisches Verfahren |
| CN107075463A (zh) | 2014-05-23 | 2017-08-18 | 赢创德固赛有限公司 | 酰基氨基酸的生物合成生产 |
| EP2949214A1 (en) | 2014-05-26 | 2015-12-02 | Evonik Degussa GmbH | Methods of producing rhamnolipids |
| US20180066297A1 (en) | 2015-02-19 | 2018-03-08 | Evonik Degussa Gmbh | Rhamnolipid synthesis |
-
2014
- 2014-05-26 EP EP14169799.5A patent/EP2949214A1/en not_active Withdrawn
-
2015
- 2015-04-27 RU RU2016149604A patent/RU2694392C9/ru active
- 2015-04-27 BR BR112016027290-0A patent/BR112016027290B1/pt active IP Right Grant
- 2015-04-27 WO PCT/EP2015/059044 patent/WO2015180907A1/en active Application Filing
- 2015-04-27 EP EP15717517.5A patent/EP3148335B1/en active Active
- 2015-04-27 US US15/311,850 patent/US10174353B2/en active Active
- 2015-04-27 JP JP2016569762A patent/JP6742918B2/ja active Active
- 2015-04-27 CN CN201580027827.4A patent/CN106414758B/zh active Active
- 2015-04-27 CA CA2948696A patent/CA2948696C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106414758B (zh) | 2021-01-01 |
| EP2949214A1 (en) | 2015-12-02 |
| JP2017516478A (ja) | 2017-06-22 |
| WO2015180907A1 (en) | 2015-12-03 |
| RU2016149604A3 (pt) | 2018-11-21 |
| RU2694392C2 (ru) | 2019-07-12 |
| US10174353B2 (en) | 2019-01-08 |
| CA2948696C (en) | 2022-10-04 |
| RU2016149604A (ru) | 2018-06-26 |
| CN106414758A (zh) | 2017-02-15 |
| BR112016027290B1 (pt) | 2022-07-12 |
| US20170096695A1 (en) | 2017-04-06 |
| RU2694392C9 (ru) | 2019-10-14 |
| EP3148335B1 (en) | 2020-01-01 |
| EP3148335A1 (en) | 2017-04-05 |
| CA2948696A1 (en) | 2015-12-03 |
| JP6742918B2 (ja) | 2020-08-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B25D | Requested change of name of applicant approved |
Owner name: EVONIK OPERATIONS GMBH (DE) |
|
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 27/04/2015, OBSERVADAS AS CONDICOES LEGAIS |