BR112017018704A2 - métodos para produção de células troncos neurais e usos das mesmas - Google Patents

métodos para produção de células troncos neurais e usos das mesmas

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Publication number
BR112017018704A2
BR112017018704A2 BR112017018704A BR112017018704A BR112017018704A2 BR 112017018704 A2 BR112017018704 A2 BR 112017018704A2 BR 112017018704 A BR112017018704 A BR 112017018704A BR 112017018704 A BR112017018704 A BR 112017018704A BR 112017018704 A2 BR112017018704 A2 BR 112017018704A2
Authority
BR
Brazil
Prior art keywords
neural stem
cell
producing
methods
stem cells
Prior art date
Application number
BR112017018704A
Other languages
English (en)
Inventor
Rodriguez Bago Julio
Dumitru Raluca
D Hingtgen Shawn
Original Assignee
Univ North Carolina Chapel Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina Chapel Hill filed Critical Univ North Carolina Chapel Hill
Publication of BR112017018704A2 publication Critical patent/BR112017018704A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/09—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from epidermal cells, from skin cells, from oral mucosa cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

aqui proporcionado, de acordo com algumas concretizações, é um método para produção de uma célula tronco neural induzida (insc), cujo método pode incluir uma ou mais das etapas de: proporcionar uma célula somática; introduzir em (por exemplo, por transfecção ou transdução) referida célula somática com um ácido nucléico que codifica sox2, pelo que referida célula expressa sox2; e, em seguida, transdiferenciação de referida célula somática (por exemplo, por crescimento da célula em um meio progenitor neural), para, desse modo, produzir referida célula tronco neural induzida. em algumas concretizações, a transdiferenciação é efetuada por um tempo de a partir de 1 a 10 dias, de 1 a 5 dias, de 1 a 3 dias, ou de 12 a 24, ou 48 horas.
BR112017018704A 2015-03-04 2016-03-03 métodos para produção de células troncos neurais e usos das mesmas BR112017018704A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562128247P 2015-03-04 2015-03-04
PCT/US2016/020649 WO2016141162A1 (en) 2015-03-04 2016-03-03 Methods for making neural stem cells and uses thereof

Publications (1)

Publication Number Publication Date
BR112017018704A2 true BR112017018704A2 (pt) 2018-04-17

Family

ID=56848639

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017018704A BR112017018704A2 (pt) 2015-03-04 2016-03-03 métodos para produção de células troncos neurais e usos das mesmas

Country Status (12)

Country Link
US (1) US20180051249A1 (pt)
EP (1) EP3265556A4 (pt)
JP (1) JP2018508216A (pt)
KR (1) KR20170133373A (pt)
CN (1) CN107849538A (pt)
AU (1) AU2016226203A1 (pt)
BR (1) BR112017018704A2 (pt)
CA (1) CA2978086A1 (pt)
IL (1) IL254156A0 (pt)
MX (1) MX2017011220A (pt)
SG (1) SG11201707047VA (pt)
WO (1) WO2016141162A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11027047B2 (en) 2015-03-31 2021-06-08 The University Of North Carolina At Chapel Hill Delivery vehicles for stem cells and uses thereof
CN113528446A (zh) * 2015-12-01 2021-10-22 海门雨霖细胞科技有限责任公司 化学诱导人肿瘤细胞直接重编程转化为非致瘤性细胞的培养基和药物组合物
CN108823164A (zh) * 2018-07-24 2018-11-16 九三极恒生物医药科技江苏有限公司 诱导多能干细胞向神经祖细胞分化的无血清培养体系
CN108893444A (zh) * 2018-07-24 2018-11-27 九三极恒生物医药科技江苏有限公司 诱导多能干细胞向皮层神经细胞分化的无血清培养体系
CN108949687A (zh) * 2018-07-24 2018-12-07 九三极恒生物医药科技江苏有限公司 诱导多能干细胞向运动神经细胞分化的无血清培养体系
JPWO2020213725A1 (pt) * 2019-04-17 2020-10-22
WO2020242212A1 (ko) * 2019-05-29 2020-12-03 성균관대학교산학협력단 신규한 유사신경교세포 제조용 칵테일 조성물, 이를 이용하여 제조된 신규한 유사신경교세포, 이의 제조 방법 및 이를 포함하는 신경 질환 예방 또는 치료용 세포 치료제

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100021437A1 (en) * 2008-04-07 2010-01-28 The McLean Hospital Corporation Whitehead Institute for Biomedical Research Neural stem cells derived from induced pluripotent stem cells
EP2356223B1 (en) * 2008-11-05 2016-04-20 Keio University Method for producing neural stem cells
WO2010108126A2 (en) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
WO2011111614A1 (en) * 2010-03-10 2011-09-15 Kyoto University Method of selecting induced pluripotent stem cell
RU2562111C2 (ru) * 2010-08-19 2015-09-10 Ф.Хоффманн-Ля Рош Аг Конверсия соматических клеток в индуцированные репрограмированные нейрональные стволовые клетки (ирнск)
CN102604894B (zh) * 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
US11027047B2 (en) * 2015-03-31 2021-06-08 The University Of North Carolina At Chapel Hill Delivery vehicles for stem cells and uses thereof

Also Published As

Publication number Publication date
AU2016226203A1 (en) 2017-10-05
JP2018508216A (ja) 2018-03-29
IL254156A0 (en) 2017-10-31
EP3265556A4 (en) 2018-08-22
KR20170133373A (ko) 2017-12-05
CN107849538A (zh) 2018-03-27
SG11201707047VA (en) 2017-09-28
CA2978086A1 (en) 2016-09-09
US20180051249A1 (en) 2018-02-22
WO2016141162A1 (en) 2016-09-09
EP3265556A1 (en) 2018-01-10
MX2017011220A (es) 2018-07-06

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]