BR112022011904A2 - Produção de oligossacarídeo sialilado em células hospedeiras - Google Patents

Produção de oligossacarídeo sialilado em células hospedeiras

Info

Publication number
BR112022011904A2
BR112022011904A2 BR112022011904A BR112022011904A BR112022011904A2 BR 112022011904 A2 BR112022011904 A2 BR 112022011904A2 BR 112022011904 A BR112022011904 A BR 112022011904A BR 112022011904 A BR112022011904 A BR 112022011904A BR 112022011904 A2 BR112022011904 A2 BR 112022011904A2
Authority
BR
Brazil
Prior art keywords
sialylated oligosaccharide
host cells
production
present
genetically modified
Prior art date
Application number
BR112022011904A
Other languages
English (en)
Inventor
Beauprez Joeri
Coussement Pieter
DECOENE Thomas
Vercauteren Annelies
Original Assignee
Inbiose Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inbiose Nv filed Critical Inbiose Nv
Publication of BR112022011904A2 publication Critical patent/BR112022011904A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PRODUÇÃO DE OLIGOSSACARÍDEO SIALILADO EM CÉLULAS HOSPEDEIRAS. A presente invenção está no campo técnico da biologia sintética e da engenharia metabólica. Mais particularmente, a presente invenção está no campo técnico da fermentação de células hospedeiras modificadas metabolicamente. A presente invenção descreve um método de preparação de oligossacarídeo sialilado por fermentação com uma célula geneticamente modificada, bem como a célula geneticamente modificada utilizada no método. A célula geneticamente modificada compreende pelo menos uma sequência do ácido nucleico que codifica uma enzima envolvida na síntese de oligossacarídeo sialilado e pelo menos um ácido nucleico que expressa uma proteína da membrana.
BR112022011904A 2019-12-18 2020-12-18 Produção de oligossacarídeo sialilado em células hospedeiras BR112022011904A2 (pt)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
BE201905939 2019-12-18
BE201905937 2019-12-18
BE201905935 2019-12-18
BE201905936 2019-12-18
BE201905938 2019-12-18
PCT/EP2020/086950 WO2021123113A1 (en) 2019-12-18 2020-12-18 Production of sialylated oligosaccharide in host cells

Publications (1)

Publication Number Publication Date
BR112022011904A2 true BR112022011904A2 (pt) 2022-09-06

Family

ID=74125211

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022011904A BR112022011904A2 (pt) 2019-12-18 2020-12-18 Produção de oligossacarídeo sialilado em células hospedeiras

Country Status (8)

Country Link
US (1) US20230212628A1 (pt)
EP (1) EP4077678A1 (pt)
KR (1) KR20220114632A (pt)
CN (1) CN114901825A (pt)
AU (1) AU2020409590A1 (pt)
BR (1) BR112022011904A2 (pt)
CA (1) CA3178327A1 (pt)
WO (1) WO2021123113A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117157396A (zh) * 2021-04-16 2023-12-01 因比奥斯公司 唾液酸化的二糖和/或寡糖的细胞生产
CN114350582A (zh) * 2021-12-10 2022-04-15 嘉必优生物技术（武汉）股份有限公司 一种制备具有自絮凝能力的大肠杆菌菌株的方法
DK202270078A1 (en) 2022-03-02 2023-12-04 Dsm Ip Assets Bv New sialyltransferases for in vivo synthesis of lst-a
DK181319B1 (en) 2022-03-02 2023-08-10 Dsm Ip Assets Bv Genetically engineered cells and methods comprising use of a sialyltransferase for in vivo synthesis of 3’sl
WO2023166035A2 (en) 2022-03-02 2023-09-07 Dsm Ip Assets B.V. New sialyltransferases for in vivo synthesis of 3'sl and 6'sl
DK202200591A1 (en) 2022-06-20 2024-02-15 Dsm Ip Assets Bv New sialyltransferases for in vivo synthesis of lst-c
WO2024089131A1 (en) 2022-10-25 2024-05-02 Inbiose N.V. Saccharide importers for lacto-n-triose

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2796082B1 (fr) 1999-07-07 2003-06-27 Centre Nat Rech Scient Procede de production d'oligosaccharides
DK1991690T3 (da) 2006-03-09 2014-04-14 Centre Nat Rech Scient Fremgangsmåde til fremstilling af sialylerede oligosaccharider
MY182355A (en) * 2009-06-08 2021-01-20 Chr Hansen Hmo Gmbh Hmo synthesis
CN107603935A (zh) 2010-07-12 2018-01-19 因比奥斯公司 用于生产附加值生物产品的代谢改造的生物
EP3517631B9 (en) 2011-12-16 2023-10-04 Inbiose N.V. Mutant microorganisms to synthesize ph sensitive molecules and organic acids
EP2859112A4 (en) 2012-06-08 2015-10-28 Glycom As PROCESS FOR THE PREPARATION OF OLIGOSACCHARIDES AND OLIGOSACCHARIDE GLYCOSIDES BY FERMENTATION
US9816122B2 (en) 2012-09-25 2017-11-14 Glycom A/S Glycoconjugate synthesis
EP3218509B1 (en) 2014-11-14 2024-02-28 Inbiose N.V. Mutant microorganisms resistant to lactose killing
EP3141610A1 (en) * 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
US10829508B2 (en) 2015-12-18 2020-11-10 Glycom A/S Fermentative production of oligosaccharides
WO2018022225A1 (en) 2016-07-26 2018-02-01 Intel IP Corporation Device for and method of radio access technology selection among multiple radio access technologies
RU2019117393A (ru) 2016-12-27 2020-12-07 Инбиоз Н.В. Синтез сиалированных соединений in vivo

Also Published As

Publication number Publication date
CA3178327A1 (en) 2021-06-24
EP4077678A1 (en) 2022-10-26
WO2021123113A1 (en) 2021-06-24
KR20220114632A (ko) 2022-08-17
CN114901825A (zh) 2022-08-12
US20230212628A1 (en) 2023-07-06
AU2020409590A1 (en) 2022-05-19

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]