BR112021017091A2 - Célula microbiana geneticamente modificada, uso da referida célula, método para a produção fermentativa de um carboidrato de interesse e uso do mesmo - Google Patents
Célula microbiana geneticamente modificada, uso da referida célula, método para a produção fermentativa de um carboidrato de interesse e uso do mesmoInfo
- Publication number
- BR112021017091A2 BR112021017091A2 BR112021017091A BR112021017091A BR112021017091A2 BR 112021017091 A2 BR112021017091 A2 BR 112021017091A2 BR 112021017091 A BR112021017091 A BR 112021017091A BR 112021017091 A BR112021017091 A BR 112021017091A BR 112021017091 A2 BR112021017091 A2 BR 112021017091A2
- Authority
- BR
- Brazil
- Prior art keywords
- carbohydrate
- interest
- cell
- genetically modified
- fermentative production
- Prior art date
Links
- 150000001720 carbohydrates Chemical class 0.000 title abstract 5
- 230000000813 microbial effect Effects 0.000 title abstract 5
- 238000012262 fermentative production Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 235000014633 carbohydrates Nutrition 0.000 abstract 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- 150000002772 monosaccharides Chemical class 0.000 abstract 2
- 239000002994 raw material Substances 0.000 abstract 2
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7004—Monosaccharides having only carbon, hydrogen and oxygen atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7016—Disaccharides, e.g. lactose, lactulose
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
produção fermentativa de carboidratos por células microbianas utilizando uma matéria prima mista. são reveladas células microbianas geneticamente modificadas para a produção de um carboidrato de interesse, em que as células microbianas possuem uma disponibilidade intracelular aumentada de pelo menos um fosfato de açúcar e produzem o carboidrato de interesse ao serem cultivadas em um meio de cultura contendo uma matéria-prima de monossacarídeo misto como principal fonte de carbono e energia. também é revelado um método fermentativo de produção de um carboidrato de interesse pelo cultivo da referida célula microbiana geneticamente modificada na presença de uma matéria-prima de monossacarídeo misto como principal fonte de carbono e energia.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19160379.4A EP3702468A1 (en) | 2019-03-01 | 2019-03-01 | Fermentative production of carbohydrates by microbial cells utilizing a mixed feedstock |
| PCT/EP2020/055299 WO2020178178A1 (en) | 2019-03-01 | 2020-02-28 | Fermentative production of carbohydrates by microbial cells utilizing a mixed feedstock |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021017091A2 true BR112021017091A2 (pt) | 2021-11-03 |
Family
ID=65861193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021017091A BR112021017091A2 (pt) | 2019-03-01 | 2020-02-28 | Célula microbiana geneticamente modificada, uso da referida célula, método para a produção fermentativa de um carboidrato de interesse e uso do mesmo |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20220145342A1 (pt) |
| EP (2) | EP3702468A1 (pt) |
| JP (1) | JP2022522366A (pt) |
| KR (1) | KR20220012834A (pt) |
| CN (1) | CN113474463A (pt) |
| AU (1) | AU2020230967A1 (pt) |
| BR (1) | BR112021017091A2 (pt) |
| MX (1) | MX2021010527A (pt) |
| SG (1) | SG11202109034VA (pt) |
| WO (1) | WO2020178178A1 (pt) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116249781A (zh) * | 2020-08-10 | 2023-06-09 | 因比奥斯公司 | 在细胞中的含有GlcNAc的生物产品的产生 |
| CN112175893B (zh) * | 2020-09-04 | 2022-06-17 | 清华大学 | 一种产唾液酸的重组微生物及其应用 |
| DK181497B1 (en) * | 2021-05-17 | 2024-03-12 | Dsm Ip Assets Bv | ENHANCING FORMATION OF THE HMOS LNT AND/OR LNnT BY MODIFYING LACTOSE IMPORT IN THE CELL |
| CN113234655B (zh) * | 2021-05-25 | 2023-09-26 | 北京化工大学 | 利用木糖生产聚-3-羟基丁酸酯的重组细菌及其制备方法和应用 |
| CN114317307B (zh) * | 2021-12-30 | 2024-05-10 | 广州智特奇生物科技股份有限公司 | 一种可提高虾青素生物合成产量的基因工程菌及其构建方法与应用 |
| CN114686541A (zh) * | 2022-02-28 | 2022-07-01 | 北京焉支山科技有限公司 | 一种化妆品级己糖-6-磷酸组合物的生物酶法合成方法及其应用 |
| CN115074376B (zh) * | 2022-04-28 | 2023-11-14 | 福州大学 | 一种利用重组大肠杆菌发酵高效合成d-阿洛酮糖的方法 |
| WO2024020530A1 (en) * | 2022-07-22 | 2024-01-25 | Debut Biotechnology, Inc. | Udp-sugar bioproduction using microorganism hosts |
| KR20240095037A (ko) | 2022-12-14 | 2024-06-25 | (주)에이피테크놀로지 | 코리네박테리움 글루타미쿰을 이용한 락토-n-푸코펜타오스의 생산 방법 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2796082B1 (fr) | 1999-07-07 | 2003-06-27 | Centre Nat Rech Scient | Procede de production d'oligosaccharides |
| KR101035043B1 (ko) | 2002-07-01 | 2011-05-19 | 아르키온 라이프 사이언씨즈 엘엘씨 | 글루코사민 및 ｎ-아세틸글루코사민의 제조를 위한 물질및 공정 |
| WO2006034225A2 (en) | 2004-09-17 | 2006-03-30 | Neose Technologies, Inc. | Production of oligosaccharides by microorganisms |
| US9150868B2 (en) | 2008-11-05 | 2015-10-06 | Mitsui Chemicals, Inc. | Bacterium producing 2-deoxy-scyllo-inosose (DOI) and method of producing 2-deoxy-scyllo-inosose (DOI) by using same |
| RU2517602C2 (ru) * | 2009-06-08 | 2014-05-27 | Йенневайн Биотехнологи Гмбх | Синтез нмо |
| US20110124063A1 (en) | 2009-11-20 | 2011-05-26 | Opx Biotechnologies, Inc. | Methods, Systems, and Compositions for Microbial Bio-Production of Biomolecules Using Syngas Components, or Sugars, as Feedstocks |
| EP3235907B1 (en) | 2010-07-12 | 2023-06-21 | Inbiose N.V. | Metabolically engineered organisms for the production of added value bio-products |
| US9029136B2 (en) * | 2012-07-25 | 2015-05-12 | Glycosyn LLC | Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides |
| EP2769726B1 (en) * | 2013-02-21 | 2018-10-31 | Jennewein Biotechnologie GmbH | Synthetic or recombinant fucosylated Oligosaccarides for use in the treatment of infections |
| EP2927316B1 (en) * | 2014-03-31 | 2018-11-07 | Jennewein Biotechnologie GmbH | Total fermentation of oligosaccharides |
| ITUB20152818A1 (it) * | 2015-08-03 | 2017-02-03 | Consorzio Univ Unifarm | Formulazioni oftalmiche a base di oligosaccaridi |
| EP3473644A1 (en) | 2017-10-17 | 2019-04-24 | Jennewein Biotechnologie GmbH | Fermentative production of n-acetylneuraminic acid |
| PL3575404T3 (pl) | 2018-05-28 | 2023-02-06 | Chr. Hansen HMO GmbH | Fermentacyjna produkcja sacharydów sialilowanych |
| PL3620510T3 (pl) | 2018-09-06 | 2024-02-19 | Chr. Hansen HMO GmbH | Fermentacyjne wytwarzanie oligosacharydów przez całkowitą fermentację z wykorzystaniem mieszanego surowca |
-
2019
- 2019-03-01 EP EP19160379.4A patent/EP3702468A1/en active Pending
-
2020
- 2020-02-28 KR KR1020217030776A patent/KR20220012834A/ko unknown
- 2020-02-28 MX MX2021010527A patent/MX2021010527A/es unknown
- 2020-02-28 JP JP2021551782A patent/JP2022522366A/ja active Pending
- 2020-02-28 SG SG11202109034VA patent/SG11202109034VA/en unknown
- 2020-02-28 US US17/434,967 patent/US20220145342A1/en active Pending
- 2020-02-28 AU AU2020230967A patent/AU2020230967A1/en active Pending
- 2020-02-28 CN CN202080017083.9A patent/CN113474463A/zh active Pending
- 2020-02-28 BR BR112021017091A patent/BR112021017091A2/pt unknown
- 2020-02-28 WO PCT/EP2020/055299 patent/WO2020178178A1/en active Application Filing
- 2020-02-28 EP EP20706522.8A patent/EP3931340A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3931340A1 (en) | 2022-01-05 |
| MX2021010527A (es) | 2021-10-01 |
| JP2022522366A (ja) | 2022-04-18 |
| EP3702468A1 (en) | 2020-09-02 |
| US20220145342A1 (en) | 2022-05-12 |
| SG11202109034VA (en) | 2021-09-29 |
| CN113474463A (zh) | 2021-10-01 |
| KR20220012834A (ko) | 2022-02-04 |
| WO2020178178A1 (en) | 2020-09-10 |
| AU2020230967A1 (en) | 2021-08-26 |
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