BR112018068981A2 - regulação da rota de sinalização de ras/camp/pka em leveduras - Google Patents

regulação da rota de sinalização de ras/camp/pka em leveduras

Info

Publication number
BR112018068981A2
BR112018068981A2 BR112018068981-5A BR112018068981A BR112018068981A2 BR 112018068981 A2 BR112018068981 A2 BR 112018068981A2 BR 112018068981 A BR112018068981 A BR 112018068981A BR 112018068981 A2 BR112018068981 A2 BR 112018068981A2
Authority
BR
Brazil
Prior art keywords
mutated
ras
camp
protein
yeast cells
Prior art date
Application number
BR112018068981-5A
Other languages
English (en)
Inventor
Michelle OESER
Brooks Henningsen
Janet FISHER
Charles F. Rice
Allan Froehlich
Aaron Argyros
Rintze M. Zelle
Original Assignee
Lallemand Hungary Liquidity Management Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Management Llc filed Critical Lallemand Hungary Liquidity Management Llc
Publication of BR112018068981A2 publication Critical patent/BR112018068981A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

o presente relatório refere-se à modulação na rota de sinalização de ras/camp/pka signaling para manter a eficiência de propagação e aumento da eficiência de fermentação de células de levedura. o presente relatório provê células de levedura apresentando ou que são engenheiradas para exibir uma modulação na sinalização em uma rota de ras/camp/pka, dependendo das condições. por exemplo, as células de levedura podem ser selecionadas ou geneticamente modificadas para expressar uma proteína ras1 mutada, uma proteína ras2 mutada, uma proteína ira1 mutada e/ou uma proteína ira2 mutada, opcionalmente em combinação com promotores específicos. são também providos métodos para a propagação de células de levedura, bem como a utilização de células de levedura para gerar um produto fermentado (tal como etanol).
BR112018068981-5A 2016-03-18 2017-03-17 regulação da rota de sinalização de ras/camp/pka em leveduras BR112018068981A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662310251P 2016-03-18 2016-03-18
US62/310,251 2016-03-18
PCT/EP2017/056456 WO2017158189A1 (en) 2016-03-18 2017-03-17 Regulation of the ras/camp/pka signaling pathway in yeasts

Publications (1)

Publication Number Publication Date
BR112018068981A2 true BR112018068981A2 (pt) 2019-04-16

Family

ID=58358617

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018068981-5A BR112018068981A2 (pt) 2016-03-18 2017-03-17 regulação da rota de sinalização de ras/camp/pka em leveduras

Country Status (7)

Country Link
US (1) US11667680B2 (pt)
EP (1) EP3430029A1 (pt)
CN (1) CN109071617A (pt)
BR (1) BR112018068981A2 (pt)
CA (1) CA3018013A1 (pt)
MX (1) MX2018011276A (pt)
WO (1) WO2017158189A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111032854A (zh) * 2017-06-13 2020-04-17 帝斯曼知识产权资产管理有限公司 重组酵母细胞
CA3091962A1 (en) 2017-09-19 2019-03-28 Lallemand Hungary Liquidity Management Llc Acetate toxicity tolerance in recombinant microbial host cells
US11326174B2 (en) * 2018-08-17 2022-05-10 Wisconsin Alumni Research Foundation Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth
IL281627B1 (en) * 2018-09-19 2024-10-01 Lallemand Usa Inc Optimization of host yeast cells for heterologous protein production
US11118017B2 (en) 2019-11-13 2021-09-14 American Process International LLC Process for the production of bioproducts from lignocellulosic material
US11306113B2 (en) 2019-11-13 2022-04-19 American Process International LLC Process for the production of cellulose, lignocellulosic sugars, lignosulfonate, and ethanol
CN110904087B (zh) * 2019-12-28 2021-05-11 浙江工业大学 L-阿拉伯糖差向异构酶突变体及其应用
CN113817662B (zh) * 2021-10-08 2023-08-29 徐州工程学院 用于提高微生物法制备3-羟基丙酸产率的诱导剂

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
AUPS334602A0 (en) * 2002-06-28 2002-07-25 Unisearch Limited Glutathione production
CA2664646C (en) 2006-10-02 2016-09-20 Dsm Ip Assets B.V. Metabolic engineering of arabinose- fermenting yeast cells
DE102007016534B4 (de) 2007-04-05 2019-03-28 Butalco Gmbh Neuer Expressionsvektor von Codon-optimierten Genen eines Arabinose-Stoffwechselweges zur Arabinose-Umsetzung in Hefe
US20100304454A1 (en) * 2007-07-19 2010-12-02 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
DE102008031350B4 (de) 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
CA3152266A1 (en) 2010-06-03 2011-12-08 Lallemand Hungary Liquidity Management Llc Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
CN104039806A (zh) 2011-11-10 2014-09-10 马斯科马公司 被加工为发酵木糖和阿拉伯糖的啤酒酵母的遗传修饰菌株
US9920312B2 (en) 2014-08-11 2018-03-20 Lallemand Hungary Liquidity Management Llc Mutations in iron-sulfur cluster proteins that improve xylose utilization

Also Published As

Publication number Publication date
US11667680B2 (en) 2023-06-06
WO2017158189A1 (en) 2017-09-21
EP3430029A1 (en) 2019-01-23
CN109071617A (zh) 2018-12-21
MX2018011276A (es) 2019-07-04
CA3018013A1 (en) 2017-09-21
US20190106464A1 (en) 2019-04-11

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]