BR112016025791A2 - microrganismo modificado, método para produzir um composto orgânico e uso de um microrganismo modificado - Google Patents

microrganismo modificado, método para produzir um composto orgânico e uso de um microrganismo modificado

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Publication number
BR112016025791A2
BR112016025791A2 BR112016025791A BR112016025791A BR112016025791A2 BR 112016025791 A2 BR112016025791 A2 BR 112016025791A2 BR 112016025791 A BR112016025791 A BR 112016025791A BR 112016025791 A BR112016025791 A BR 112016025791A BR 112016025791 A2 BR112016025791 A2 BR 112016025791A2
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BR
Brazil
Prior art keywords
modified microorganism
gene
reduced activity
producing
enzyme encoded
Prior art date
Application number
BR112016025791A
Other languages
English (en)
Inventor
Schroeder Hartwig
Martyna KRAWCZYK Joanna
Zelder Oskar
Haefner Stefan
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of BR112016025791A2 publication Critical patent/BR112016025791A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01018—Inositol 2-dehydrogenase (1.1.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y197/00—Other oxidoreductases (1.97)
    • C12Y197/01—Other oxidoreductases (1.97) other oxidoreductases (1.97.1)
    • C12Y197/01004—[Formate-C-acetyltransferase]-activating enzyme (1.97.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0104—Pyruvate kinase (2.7.1.40)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08031—Undecaprenyl-phosphate glucose phosphotransferase (2.7.8.31)

Abstract

a presente invenção refere-se a um microrganismo modificado que tem, em comparação ao seu tipo selvagem, - uma atividade reduzida de uma enzima codificada pelo gene ptsa, - uma atividade reduzida de uma enzima codificada pelo gene ptsh ou - uma atividade reduzida de uma enzima codificada pelo gene ptsa e uma atividade reduzida de uma enzima codificada pelo gene ptsh, em que o tipo selvagem a partir do qual o microrganismo modificado foi derivado pertence à família pasteurellaceae. a presente invenção também se refere a um método para produzir ácido succínico e ao uso de microrganismos modificados.
BR112016025791A 2014-05-08 2015-05-07 microrganismo modificado, método para produzir um composto orgânico e uso de um microrganismo modificado BR112016025791A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14167488 2014-05-08
PCT/EP2015/060102 WO2015169920A1 (en) 2014-05-08 2015-05-07 Genetically modified microorganism for improved production of fine chemicals on sucrose

Publications (1)

Publication Number Publication Date
BR112016025791A2 true BR112016025791A2 (pt) 2018-01-16

Family

ID=50679920

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016025791A BR112016025791A2 (pt) 2014-05-08 2015-05-07 microrganismo modificado, método para produzir um composto orgânico e uso de um microrganismo modificado

Country Status (9)

Country Link
US (1) US10287571B2 (pt)
EP (1) EP3140412A1 (pt)
JP (1) JP6608392B2 (pt)
KR (1) KR102308323B1 (pt)
CN (1) CN106536717A (pt)
BR (1) BR112016025791A2 (pt)
CA (1) CA2947663A1 (pt)
MY (1) MY193587A (pt)
WO (2) WO2015169919A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106661595A (zh) * 2014-03-19 2017-05-10 巴斯夫欧洲公司 在发酵中使用甘油和限制进料的碳水化合物
US10301653B2 (en) 2015-07-06 2019-05-28 Wisconsin Alumni Research Foundation Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use
WO2017093450A1 (en) 2015-12-02 2017-06-08 Basf Se Method of producing proteins in filamentous fungi with decreased clr2 activity
MX2018006536A (es) 2015-12-02 2018-08-15 Basf Se Metodo para producir proteinas en hongos filamentosos con accion clr1 reducida.
WO2018183381A1 (en) 2017-03-27 2018-10-04 Tenfold Technologies, LLC Methods and agricultural compositions for preventing or controlling plant diseases
WO2018183383A1 (en) * 2017-03-27 2018-10-04 Tenfold Technologies, LLC Anti-pathogen composition and methods of use thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100679638B1 (ko) * 2005-08-19 2007-02-06 한국과학기술원 포메이트 디하이드로게나제 ｄ 또는 ｅ를 코딩하는 유전자로 형질전환된 미생물 및 이를 이용한 숙신산의 제조방법
US20130305398A1 (en) * 2012-02-16 2013-11-14 Marie Coffin Genes and uses for plant enhacement
BRPI0815409B1 (pt) 2007-08-17 2023-01-24 Basf Se Processos para a produção fermentativa de um composto, e para a produção de um composto, e, uso de uma cepa bacteriana
EP2202294B1 (en) * 2008-12-23 2015-10-21 Basf Se Bacterial cells having a glyoxylate shunt for the manufacture of succinic acid
EP2396401B1 (en) * 2009-02-16 2018-12-19 Basf Se Novel microbial succinic acid producers and purification of succinic acid
US20120058530A1 (en) * 2009-04-02 2012-03-08 University Of Florida Research Foundation Inc. Engineering the pathway for succinate production
US8778656B2 (en) * 2009-11-18 2014-07-15 Myriant Corporation Organic acid production in microorganisms by combined reductive and oxidative tricaboxylic acid cylce pathways
KR101221557B1 (ko) * 2010-08-30 2013-01-14 한국과학기술원 수크로오즈와 글리세롤을 동시에 이용하는 신규 숙신산 생성 변이 미생물 및 이를 이용한 숙신산 제조방법
WO2012031079A2 (en) * 2010-09-01 2012-03-08 University Of Florida Research Foundation, Inc. L-malate production by metabolically engineered escherichia coli
WO2013003432A1 (en) * 2011-06-29 2013-01-03 Genomatica, Inc. Microorganisms for producing succinate and methods related thereto
US9029136B2 (en) * 2012-07-25 2015-05-12 Glycosyn LLC Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides
EP2909325A4 (en) * 2012-10-22 2016-05-25 Genomatica Inc MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND THE PRODUCTION OF SUCCINATE CORRESPONDING

Also Published As

Publication number Publication date
KR102308323B1 (ko) 2021-10-06
WO2015169920A1 (en) 2015-11-12
MY193587A (en) 2022-10-19
JP2017514511A (ja) 2017-06-08
CN106536717A (zh) 2017-03-22
JP6608392B2 (ja) 2019-11-20
CA2947663A1 (en) 2015-11-12
EP3140412A1 (en) 2017-03-15
KR20170003630A (ko) 2017-01-09
US20170073665A1 (en) 2017-03-16
WO2015169919A1 (en) 2015-11-12
US10287571B2 (en) 2019-05-14

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time