EA201792544A1 - Генетически сконструированные микроорганизмы для получения происходящих из хоризмата продуктов - Google Patents

Генетически сконструированные микроорганизмы для получения происходящих из хоризмата продуктов

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Publication number
EA201792544A1
EA201792544A1 EA201792544A EA201792544A EA201792544A1 EA 201792544 A1 EA201792544 A1 EA 201792544A1 EA 201792544 A EA201792544 A EA 201792544A EA 201792544 A EA201792544 A EA 201792544A EA 201792544 A1 EA201792544 A1 EA 201792544A1
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EA
Eurasian Patent Office
Prior art keywords
horizmat
microorganisms
exogenous
obtaining products
genetically designed
Prior art date
Application number
EA201792544A
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English (en)
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EA036334B9 (ru
EA036334B1 (ru
Inventor
Джеймс Брюс Ярнтон Берендорфф
Михель Кёпке
Лоан Пхуонг Тран
Уайатт Эрик Аллен
Original Assignee
Ланцатек Нью Зилэнд Лимитед
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Application filed by Ланцатек Нью Зилэнд Лимитед filed Critical Ланцатек Нью Зилэнд Лимитед
Publication of EA201792544A1 publication Critical patent/EA201792544A1/ru
Publication of EA036334B1 publication Critical patent/EA036334B1/ru
Publication of EA036334B9 publication Critical patent/EA036334B9/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
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    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/0304—Chorismate lyase (4.1.3.40)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/99—Other carbon-oxygen lyases (4.2.99)
    • C12Y402/99021—Isochorismate lyase (4.2.99.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/04—Intramolecular transferases (5.4) transferring hydroxy groups (5.4.4)
    • C12Y504/04002—Isochorismate synthase (5.4.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99005—Chorismate mutase (5.4.99.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Согласно настоящему изобретению предложены генетически сконструированные микроорганизмы и способы получения происходящих из хоризмата продуктов, таких как парагидроксибензойная кислота, салицилат, 2-аминобензоат, 2,3-дигидроксибензоат и 4-гидроксициклогексанкарбоновая кислота. Как правило, указанный микроорганизм содержит по меньшей мере одно из следующего: (a) экзогенную хоризмат-пируват-лиазу, (b) экзогенную изохоризматсинтазу, (c) экзогенную изохоризмат-пируват-лиазу и (d) префенатсинтазу, которая содержит нарушающую экспрессию мутацию.
EA201792544A 2015-05-27 2016-05-26 Генетически модифицированная бактерия clostridium для получения салицилата из хоризмата EA036334B9 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562167101P 2015-05-27 2015-05-27
PCT/US2016/034495 WO2016191625A1 (en) 2015-05-27 2016-05-26 Genetically engineered microorganisms for the production of chorismate-derived products

Publications (3)

Publication Number Publication Date
EA201792544A1 true EA201792544A1 (ru) 2018-05-31
EA036334B1 EA036334B1 (ru) 2020-10-28
EA036334B9 EA036334B9 (ru) 2020-12-24

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EA201792544A EA036334B9 (ru) 2015-05-27 2016-05-26 Генетически модифицированная бактерия clostridium для получения салицилата из хоризмата

Country Status (11)

Country Link
US (1) US10174303B2 (ru)
EP (1) EP3303594A4 (ru)
JP (1) JP6871870B2 (ru)
KR (1) KR102390716B1 (ru)
CN (2) CN113684167A (ru)
BR (4) BR122023023156A2 (ru)
CA (1) CA2985481C (ru)
EA (1) EA036334B9 (ru)
MY (1) MY186542A (ru)
WO (1) WO2016191625A1 (ru)
ZA (1) ZA201707234B (ru)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE052810T2 (hu) * 2014-12-08 2021-05-28 Lanzatech New Zealand Ltd Rekombináns mikroorganizmusok, amelyek megnövekedett fluxust mutatnak fermetációs útvonalon keresztül
JP2019514441A (ja) 2016-05-14 2019-06-06 ランザテク，インコーポレイテッド 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法
KR101918673B1 (ko) 2017-04-17 2018-11-14 주식회사 콧데 해양 유래 신규 미생물 및 이를 이용한 화장품 조성물
EA202090616A1 (ru) 2017-09-08 2020-06-10 Ланцатек, Инк. Способы и системы для получения метаболита с использованием обогащенных водородом с1-содержащих субстратов
KR20200050470A (ko) * 2017-09-28 2020-05-11 란자테크, 인크. 우드-륭달 미생물에서 유전자 녹아웃
KR20200091458A (ko) 2017-12-19 2020-07-30 란자테크, 인크. 에틸렌 글리콜의 생물학적 생성을 위한 미생물 및 방법
AU2019218389A1 (en) 2018-02-12 2020-10-01 Lanzatech, Inc. A process for improving carbon conversion efficiency
CA3097019A1 (en) 2018-04-20 2019-10-24 Lanzatech, Inc. Intermittent electrolysis streams
WO2020106722A1 (en) 2018-11-19 2020-05-28 Lanzatech, Inc. Integration of fermentation and gasification
CN112969795A (zh) 2019-02-08 2021-06-15 朗泽科技有限公司 用于回收沸点接近的产物的方法
SG11202111976TA (en) 2019-07-11 2021-11-29 Lanzatech Inc Methods for optimizing gas utilization
JP2021185831A (ja) * 2020-05-29 2021-12-13 花王株式会社 没食子酸生産能を有する形質転換体
CA3224707A1 (en) 2020-06-06 2021-12-09 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
AU2022218205A1 (en) 2021-02-08 2023-05-25 Lanzatech, Inc. Recombinant microorganisms and uses therefor
WO2022245340A1 (en) * 2021-05-18 2022-11-24 Zymergen Inc. Engineered biosynthetic pathways for production of deoxyhydrochorismic acid by fermentation
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57170184A (en) * 1980-07-18 1982-10-20 Unisearch Ltd Mutant of e. coli and production of phenylalanine
US6210937B1 (en) * 1997-04-22 2001-04-03 Bechtel Bwxt Idaho, Llc Development of genetically engineered bacteria for production of selected aromatic compounds
WO1999050423A2 (en) * 1998-03-31 1999-10-07 Mogen International N.V. Isochorismate synthase and its use for the induction of resistance in plants
US7056742B2 (en) * 2003-06-16 2006-06-06 E. I. Du Pont De Nemours And Company High Level production of arbutin in green plants
CN100412199C (zh) * 2006-04-29 2008-08-20 北京未名凯拓作物设计中心有限公司 一种提高植物中水杨酸含量的方法及其专用载体
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
WO2008089102A2 (en) 2007-01-12 2008-07-24 Regents Of The University Of Colorado Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms
WO2009052303A2 (en) * 2007-10-16 2009-04-23 Monsanto Technology Llc Processes for producing and recovering shikimic acid
MX2012008738A (es) * 2010-01-29 2012-08-31 Genomatica Inc Microorganismos y metodos para la biosintesis de p-toluato y tereftalato.
EA025778B1 (ru) * 2010-07-28 2017-01-30 Ланзатек Нью Зиленд Лимитед Новые бактерии и способы их применения
CN102140431B (zh) * 2010-12-21 2014-08-27 大成生化科技(松原)有限公司 L-色氨酸基因工程菌,其构建方法以及使用其发酵生产l-色氨酸的方法
US20110256600A1 (en) 2011-02-02 2011-10-20 Lanzatech New Zealand Limited Recombinant Microorganisms and Methods of Use Thereof
WO2013103894A1 (en) * 2012-01-05 2013-07-11 Paromatics, Llc Biological syntheisis of p-aminobenzoic acid, p-aminophenol, n-(4-hydroxyphenyl)ethanamide and derivatives thereof
US20130316364A1 (en) * 2012-05-23 2013-11-28 Lanzatech New Zealand Limited Selection Method and Recombinant Microorganisms and uses Therefor
CA2890922A1 (en) 2012-11-13 2014-05-22 Bayer Technology Services Gmbh Method for producing phenol from renewable resources by fermentation
US9617572B2 (en) * 2012-12-31 2017-04-11 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via aromatic compounds
FI2951286T3 (fi) * 2013-01-30 2023-11-20 Lanzatech Nz Inc Rekombinantteja mikrobeja, jotka käsittävät NAPDH:sta riippuvaisia entsyymejä, ja niiden tuotantomenetelmiä
US9353390B2 (en) * 2013-06-13 2016-05-31 University Of Georgia Research Foundation, Inc. Genetically engineered microbes and methods for producing 4-hydroxycoumarin
WO2015069847A2 (en) * 2013-11-06 2015-05-14 Massachusetts Institute Of Technology Co-culture based modular engineering for the biosynthesis of isoprenoids, aromatics and aromatic-derived compounds

Also Published As

Publication number Publication date
KR102390716B1 (ko) 2022-04-26
WO2016191625A1 (en) 2016-12-01
BR122023023155A2 (pt) 2024-03-05
US20160348087A1 (en) 2016-12-01
BR122023023156A2 (pt) 2024-03-05
US10174303B2 (en) 2019-01-08
BR122023023164A2 (pt) 2024-03-05
EA036334B9 (ru) 2020-12-24
CA2985481C (en) 2019-04-30
KR20180002704A (ko) 2018-01-08
EP3303594A4 (en) 2018-12-05
CN113684167A (zh) 2021-11-23
CN107636147A (zh) 2018-01-26
MY186542A (en) 2021-07-26
EA036334B1 (ru) 2020-10-28
CA2985481A1 (en) 2016-12-01
BR112017025309A2 (pt) 2018-07-31
JP2018522536A (ja) 2018-08-16
EP3303594A1 (en) 2018-04-11
CN107636147B (zh) 2021-09-10
JP6871870B2 (ja) 2021-05-19
ZA201707234B (en) 2019-12-18

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