CN109906270A - 经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺 - Google Patents

经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺 Download PDF

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CN109906270A
CN109906270A CN201780066177.3A CN201780066177A CN109906270A CN 109906270 A CN109906270 A CN 109906270A CN 201780066177 A CN201780066177 A CN 201780066177A CN 109906270 A CN109906270 A CN 109906270A
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阿琳·M·福斯默
克里斯托弗·K·米勒
格雷戈里·迈克尔·波因特
布莱恩·杰弗里·拉什
乔恩·维尔德豪斯
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Abstract

本发明涉及可使用乳酸作为碳源来产生发酵产物的经遗传修饰的酵母。在一个方面，所述酵母可同时消耗葡萄糖和乳酸以产生乙醇。在一个方面，转化所述经遗传修饰的酵母以包含一元羧酸/单羧酸转运蛋白。在一个方面，所述酵母可包含一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(EC 1.1.2.3和/或1.1.2.4)。

Description

经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母 的发酵工艺
相关申请的交叉引用
本申请要求2016年9月16日提交的题为“GENETICALLY MO DIFIED LACTATE-CONSUMING YEASTS AND FERMENTATION PROCESSES USING SUCH GENETICALLY MODIFIEDYEAST(经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺)”的美国临时专利申请序列号62/395,792的权益，该临时专利申请以引用方式整体并入本文。
序列表
2017年9月15日创建的具有506千字节大小的题为“N00478_ST25.txt”的ASCII文本文件的全部内容以引用方式整体并入本文。
发明背景
工业酵母发酵工艺通常使用玉米工艺物流，例如玉米浸泡水，作为发酵底物。这些工艺物流包含可通过糖化酶处理转变为糖的淀粉。在当前的发酵工艺中，葡萄糖或其他糖用作发酵的碳源。然而，此类工艺物流可包含大量乳酸，乳酸不能被酵母有效地用作碳源，当存在葡萄糖时尤其如此。
发明内容
本文描述了可在发酵期间消耗乳酸的经遗传修饰的酵母。在一个方面，酵母可使用乳酸作为碳源以形成发酵产物。在一个方面，酵母可在发酵工艺期间同时消耗乳酸和葡萄糖以产生发酵产物。在一些实施方案中，发酵产物是乙醇(EtOH)。在一些实施方案中，酵母是经遗传修饰的酿酒酵母(Saccharomyces cerevisiae)酵母。在一些实施方案中，转化酵母以表达异源单羧酸/质子同向转运蛋白，例如JEN1同向转运蛋白。在一些实施方案中，转化酵母以表达一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(EC 1.1.2.3或1.1.2.4)。
在一个方面，经遗传修饰的酵母包含编码一元羧酸/单羧酸转运蛋白的异源基因和一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(分类为EC 1.1.2.3或1.1.2.4)，其中当酵母存在于包含乳酸和己糖的发酵培养基中时，酵母能够消耗乳酸并产生乙醇。在一些实施方案中，酵母能够消耗D-乳酸、L-乳酸或它们的混合物。在一些实施方案中，酵母能够消耗D-乳酸和L-乳酸两者。在一些实施方案中，当发酵培养基中己糖的浓度为150g/L或更高时，酵母具有至少0.030、0.035、0.040、0.045或0.050g L-1h-1的L-乳酸消耗速率。在一些实施方案中，当发酵培养基中己糖的浓度为5g/L或更高时，酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的L-乳酸消耗速率。在一些实施方案中，当发酵培养基中己糖的浓度为150g/L或更高时，酵母具有至少0.005、0.006、0.007、0.008、0.009、0.010、0.015、0.020、0.025或0.030gL^h-1的D-乳酸消耗速率。在一些实施方案中，当发酵培养基中己糖的浓度为5g/L或更高时，酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的D-乳酸消耗速率。在一些实施方案中，根据本文稍后描述的“乳酸消耗的评估方案”来确定酵母的D-乳酸、L-乳酸或总乳酸的乳酸消耗速率。
在一些实施方案中，在同时糖化发酵(SSF)工艺中的0与7小时时间之间酵母具有至少0.550、0.555、0.600或0.650gL^h-1的总乳酸消耗速率(即，D-乳酸和L-乳酸两者的消耗速率)。在一些实施方案中，在SSF工艺中的7小时与48小时时间之间酵母具有至少0.130、0.140、0.150、0.160或0.170gL～3/4-1的总乳酸消耗速率。在一些实施方案中，在SSF工艺中的0与48小时时间之间酵母具有至少0.200、0.210、0.220、0.230、0.240或0.250gL^h-1的总乳酸消耗速率。在一些实施方案中，根据本文稍后描述的实施例4中的SSF摇瓶测定的方法来确定总乳酸消耗速率。
在一个方面，一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的D-乳酸脱氢酶(DLD)基因(EC 1.1.2.4)。在一个方面，一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的细胞色素b2(CYB2)基因(EC 1.1.2.3)。在一些实施方案中，一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的DLD基因和过表达的CYB2基因。在一些实施方案中，一种或多种DLD基因来自以下酵母属：酵母属(Saccharomyces)、伊萨酵母属(Issatchenkia)和/或克鲁维酵母属(Kluyveromyces)。在一些实施方案中，一种或多种DLD基因编码DLD多肽，所述DLD多肽具有与来自以下酵母物种中的一种或多种的DLD多肽具有至少90％或95％的序列同一性的氨基酸序列：酿酒酵母、东方伊萨酵母(Issatchenkiaorientalis)、克鲁弗酵母(Saccharyomyces kluyveri)、贝酵母(Saccharyomycesbayanus)、多布克鲁维酵母(Kluyveromyces dobzhanskii)、马克斯克鲁维酵母(Kluyveromyces marxianus)或乳酸克鲁维酵母(Kluyveromyces lactis)。在一些实施方案中，酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ IDNO:40、SEQ ID NO:42、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ IDNO:72或SEQ ID NO:74。
在一些实施方案中，一种或多种CYB2基因来自以下酵母属：酵母属、伊萨酵母属、接合酵母属(Zygosaccharomyces)和/或克鲁维酵母属。在一些实施方案中，一种或多种CYB2基因编码CYB2多肽，所述CYB2多肽具有与来自以下酵母物种中的一种或多种的CYB2多肽具有至少90％或95％的序列同一性的氨基酸序列：酿酒酵母、东方伊萨酵母、克鲁弗酵母、贝酵母、鲁氏接合酵母(Zygosaccharomyces rouxii)、多布克鲁维酵母或乳酸克鲁维酵母。在一些实施方案中，酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:15、SEQ ID NO:17、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ IDNO:60或SEQ ID NO:62。
在一个方面，酵母包含除DLD、CYB2或异源单羧酸/质子同向转运蛋白之外的异源基因。在一些实施方案中，酵母包含编码乳酸消旋酶的异源基因。
在一个方面，经遗传修饰的酵母包含编码单羧酸/质子同向转运蛋白(JEN1)的异源基因和编码葡糖淀粉酶的异源基因，其中当酵母存在于包含乳酸并且还包含己糖、淀粉或它们的组合的发酵培养基中时，酵母能够消耗乳酸并产生乙醇。在一个方面，上述任何酵母可用异源葡糖淀粉酶基因转化。在一些实施方案中，酵母可编码来自扣囊复膜酵母(Saccharomycopsis fibuligera)或米根霉(Rhizopus oryzae)的葡糖淀粉酶。在一些实施方案中，酵母编码与来自扣囊复膜酵母的野生型葡糖淀粉酶多肽具有至少80％、85％、90％或95％的序列同一性的葡糖淀粉酶多肽。可由酵母编码的葡糖淀粉酶多肽以及编码可整合在酵母中的这些肽的DNA序列的实施方案在2017年8月04日提交的国际专利申请号PCT/US17/045493中进一步描述，所述专利申请以引用方式整体并入本文，包括与其相关的所有SEQ ID。
在一个方面，经遗传修饰的宿主酵母属于酵母属种。在一些实施方案中，宿主酵母是酿酒酵母。在一些实施方案中，经遗传修饰的酵母包含酿酒酵母，其中酵母包含编码单羧酸/质子同向转运蛋白(例如JEN1)的异源基因。在一些实施方案中，宿主酵母是可商购获得的用于乙醇生产的酿酒酵母，诸如ETHANOL REDTM酵母，该酵母在本公开期间可从Lesaffre或其子公司获得。
在一个方面，酵母能够以至少1.0g L-1h-1、2.0g L-1h-1、3.0g L-1h-1、3.3g L-1h-1或3.75g L-1h-1的发酵生产速率产生乙醇。在一个方面，酵母能够以大于80g/L、90g/L、100g/L、110g/L、120g/L、125g/L 130g/L、135g/L、140g/L、145g/L、150g/L、155g/L、160g/L或165g/L的滴度产生乙醇。在一个方面，酵母(a)能够以大于90g/L、100g/L、110g/L、120g/L、130g/L或140g/L的滴度产生乙醇；(b)在33℃至40℃、33℃至39℃、33℃至38℃、33℃至37℃、34℃至37℃、35℃至37℃或36℃至38℃范围内的温度下耐热；或(a)和(b)两者兼而有之。
在一个方面，编码一元羧酸/单羧酸转运蛋白的异源基因来自克鲁维酵母属的酵母。在一些实施方案中，编码一元羧酸/单羧酸转运蛋白的异源基因来自乳酸克鲁维酵母。在一些实施方案中，酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:76或SEQ ID NO:78。在一些实施方案中，酵母包含在SEQ ID NO:15、SEQ ID NO:17、SEQ ID NO:54、SEQ ID NO:56、SEQ IDNO:58、SEQ ID NO:60或SEQ ID NO:62中的指定位置处的以下残基中的一者或多者：Lys349、Tyrl43、Tyr254和His373。在一个方面，酵母用于产生乙醇或除乙醇之外的生物产物。
在一个方面，本公开涉及使用经遗传修饰的酵母，例如上述任何经遗传修饰的酵母的发酵工艺。在一些实施方案中，所述工艺是使用此类经遗传修饰的酵母使培养基发酵以生产乙醇的任何工艺。在一些实施方案中，所述工艺是一种用于制造乙醇的连续发酵工艺，所述工艺包括：提供包含己糖或己糖低聚物的发酵培养基；用包含异源单羧酸/质子同向转运蛋白(JENl)基因的经遗传修饰的酵母使发酵培养基发酵；向发酵培养基中添加一种或多种包含乳酸的进料物流；并从发酵培养基中移出至少一种包含乙醇的输出物流，其中发酵培养基的平均己糖或己糖低聚物浓度为至少5g/L，工艺的体积氧摄取速率为至少0.5mmol 02/(L-h)，并且所述工艺以至少1.0g L-1h-1的总速率产生乙醇且酵母消耗乳酸，其中至少一种输出物流含有小于90％的在一种或多种进料物流中添加的乳酸。在一些实施方案中，一种或多种进料物流的乳酸浓度具有至少1g/L的平均乳酸浓度。在一些实施方案中，进料物流是植物工艺物流。在一些实施方案中，植物工艺物流是玉米工艺物流或小麦工艺物流。在一些实施方案中，在预发酵器步骤结束时的乙醇滴度在20至80g/L的范围内。在一些实施方案中，繁殖器步骤和/或预发酵器步骤中的乳酸消耗速率为至少0.550、0.600或0.650g L-1h-1。在一些实施方案中，至少一种输出物流的乙醇滴度为至少90、100、110、120、130、140、150或160g/L。
在一些实施方案中，所述工艺是一种用于制造乙醇的工艺，所述工艺包括：使用包含异源单羧酸/质子同向转运蛋白(JENl)基因的经遗传修饰的酵母使培养基发酵，其中在发酵开始时培养基包含浓度为至少5g/L的葡萄糖或葡萄糖低聚物，在发酵开始时培养基的乳酸含量为至少1g/L，在发酵结束时至少35％、40％、45％、50％或更多的乳酸被消耗，并且最终乙醇滴度为至少90g/L。在一些实施方案中，所述工艺具有至少0.5、1、2、3、4、5、6、7、8、9、10、20、50或100mmol 02/(L-h)的体积氧摄取速率(OUR)。
在一个方面，乳酸可以是L-乳酸、D-乳酸或它们的混合物。在一些实施方案中，发酵培养基的pH在约2至7的范围内。在一些实施方案中，发酵温度在25℃至45℃、25℃至40℃、25℃至35℃、25℃至30℃、30℃至45℃、30℃至40℃、25℃至38℃、28℃至38℃或30℃至39v℃的范围内。在一些实施方案中，所述工艺的最终乙醇滴度为至少80、90、100、110、120、130、135、140、145、150、155或160g/L。
在一些实施方案中，发酵培养基的干固体在发酵培养基中为至少30、40、50、60、70或80g/L。在一些实施方案中，发酵进料的干固体为至少120、130、140或150g/L。
在一个方面，所述工艺是一种用于制造乙醇的工艺，所述工艺包括：使用酿酒酵母(例如Ethanol RedTM酵母，可从Lesaffre商购获得)使培养基发酵，其中培养基包含葡萄糖或葡萄糖低聚物和乳酸，OUR为至少0.5、1、2、3、4、5、6、7、8、9或10mmol 02/(L-h)，在发酵开始时培养基的乳酸含量为至少1、2、3或4g/L，在发酵结束时至少35％、40％、45％或50％的乳酸被消耗，并且最终乙醇滴度为至少90g/L
在一个方面，所述方法是一种用于产生具有改善的乳酸消耗的经遗传修饰的酵母的方法，所述方法包括过表达一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(分类为EC 1.1.2.3或1.1.2.4)或异源单羧酸/质子同向转运蛋白，并使酵母经历进化以获得特征，其中当酵母存在于包含乳酸和葡萄糖的发酵培养基中时，酵母能够消耗乳酸并产生乙醇。在一些实施方案中，所述特征是在乳酸存在下酵母的生长速率增加和/或酵母的乙醇产量增加。
在一个方面，在上述任何方法或工艺或酵母中，酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQID NO:76或SEQ ID NO:78。在一些实施方案中，酵母包含在SEQ ID NO:15、SEQ ID NO:17、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62中的指定位置处的以下残基中的一者或多者：Lys349、Tyrl43、Tyr254和His373。
在一个方面，在上述任何方法或工艺或酵母中，酵母包括与编码一元羧酸/单羧酸转运蛋白的异源基因相关的异源核酸调控序列。在一些实施方案中，异源核酸调控序列包含ADH1启动子。在一些实施方案中，异源核酸调控序列包含ADH2启动子、PDC1启动子或GPD1启动子。
在一个方面，经遗传修饰的酵母适用于商业上可接受的性能下的乙醇生产。因此，在上述任何实施方案中，酵母和使用酵母的工艺与商业上相关的最终乙醇滴度、发酵结束时的乙醇滴度或从工艺中移出的乙醇浓度为至少90、100、110、120、130、140、150或160g/L的工艺物流或材料相关联。此外，在一些实施方案中，上述任何酵母具有的D-乳酸、L-乳酸和/或总乳酸消耗速率大于不具有编码一元羧酸/单羧酸转运蛋白的异源基因的酵母。
在一个方面，包含上文或本文其他地方描述的编码一元羧酸/单羧酸转运蛋白的异源基因的任何经遗传修饰的酵母被描述为能够在存在葡萄糖时，即当处于包含葡萄糖的发酵培养基中时消耗乳酸并产生乙醇。在一些实施方案中，发酵培养基包含可测量的非零浓度的葡萄糖。然而，本文预期在一些实施方案中，发酵培养基的葡萄糖浓度可能实际上不可测量，因为葡萄糖可添加到发酵培养基中或以其他方式在发酵培养基内从葡萄糖低聚物产生，这样存在于发酵培养基中的酵母会立即消耗葡萄糖。因此，应理解“包含葡萄糖的发酵培养基”可包括这样的发酵培养基：其中发酵培养基中一添加或产生了葡萄糖，葡萄糖就被酵母消耗，导致葡萄糖实际上不可测量。
还应理解，如本领域技术人员将理解的，上述工艺、方法或组合物的任何实施方案的要素或方面可应用于任何其他实施方案。
附图说明
结合附图一起阅读时，将更好地理解本发明的以下详细说明。然而，应当理解，本发明并不限于在附图中所示的实施方案的精确安排和手段。
图1(包括图1A至图1C)是一组图，这组图示出了发酵摇瓶测定中酿酒酵母参考菌株(1和1-10)和含有过表达的CYB2和JEN1的菌株(1-24)的葡萄糖消耗、乙醇产生和L-乳酸消耗。
图2(包括图2A至图2C)是一组图，这组图示出了发酵摇瓶测定中酿酒酵母参考菌株(1和1-10)和含有过表达的乳酸克鲁维酵母JEN1的菌株(1-17)的葡萄糖消耗、乙醇产生和D-乳酸消耗。
图3(包括图3A至图3C)是一组图，这组图示出了发酵摇瓶测定中酿酒酵母参考菌株(1和1-10)、含有过表达的乳酸克鲁维酵母JEN1的菌株(1-17)以及含有过表达的乳酸克鲁维酵母JEN1和过表达的ScDLDl的菌株(1-30)的葡萄糖消耗、乙醇产生和D-乳酸消耗。
图4(包括图4A和图4B)是一组图，这组图示出了同时糖化发酵(SSF)摇瓶测定中酿酒酵母参考菌株(1和1-10)和含有过表达的CYB2b和JEN1的菌株(1-25)的D,L-乳酸消耗和乙醇产生。
图5(包括图5A和图5B)是一组图，这组图示出了SSF摇瓶测定中酿酒酵母参考菌株(1和1-10)和含有过表达的ScCYB2和K1JEN1的两个拷贝的菌株(1-41)的D,L-乳酸消耗和乙醇产生。
图6是示出了摇瓶测定中酿酒酵母参考菌株(1和1-10)和含有过表达的酿酒酵母DLD1和酿酒酵母CYB2及选定的单羧酸/质子同向转运蛋白的菌株的L-乳酸消耗的图。
具体实施方式
应当理解，本文提供的本发明的附图和描述已经被简化，以说明与清楚理解本发明相关的要素，同时消除在相关领域中发现的其他要素。本领域普通技术人员将认识到，在实现本发明时可能需要或要求其他要素或步骤。然而，因为此类要素或步骤是本领域熟知的，或者它们不利于更好地理解本发明，所以本文不提供对此类要素或步骤的讨论。
定义
除非另外定义，否则在本文中使用的所有技术和科学术语均具有与本发明所属领域的技术人员通常理解的含义相同的含义。如本文所用，以下术语中的每一个均具有与其在本章节中的定义相关的含义。
如本文所用，术语“经遗传修饰的”、“经遗传工程化的”等是指使用任何遗传工程化技术以任何方式改变生物体的遗传物质，所述技术包括使用成簇的规律间隔的短回文重复序列(CRISPR)和CRISPR相关蛋白的技术，即CRISPR/cas系统。
如本文所用，“接种”定义为将能够产生发酵产物的微生物引入发酵培养基中的时间点。
术语“生物产物”“发酵产物”等在本文中可互换使用，并且是指由生物体通过碳源发酵产生的化合物或化合物的混合物。
如本文所用，“发酵结束”定义为发酵工艺满足预定结束点标准的时间点。预定的结束点标准可包括以下任一者：预定的时间间隔、所需分数的所供应碳源的耗尽、碳源消耗的停止或发酵产物形成的停止。在一个实施方案中，“发酵结束”定义为开始收获生物产物的时间点。如本领域技术人员将理解的，“发酵结束”可指取决于发酵工艺的规模和目的而不同的时间点。对于大规模生产发酵工艺，“发酵结束”优选是开始收获生物产物的时间点，即在产物形成已有效停止之后。如本领域技术人员将理解的，分批工艺的结束点标准适用于整个批次，而连续工艺的结束点标准可仅适用于该工艺的一部分(例如与该工艺相关的单个容器或单元操作，例如与预发酵器相关的工艺步骤)，或者可应用于整个连续工艺，即应用于连续工艺的输出参数。
如本文所用，“细胞干重”是指如在发酵样品中测量的在测量时发酵培养基中存在的干细胞群的浓度。细胞干重通常以克/升(g/L)为单位表示。
如本文所用，“接种时的细胞干重”是指如在发酵样品中测量的紧跟着接种之后在发酵培养基中存在的干细胞群的浓度。对于补料分批发酵，基于发酵培养基的最终体积计算初始细胞干重。细胞干重的测量是本领域技术人员已知的方法。接种时的细胞干重通常以g/L为单位表示。
如本文所用，“发酵结束时的细胞干重”是指如在发酵样品中测量的在发酵结束时发酵培养基中存在的干细胞群的浓度。发酵结束时的细胞干重通常以g/L为单位表示。
如本文所用，“最终滴度”是指发酵结束时发酵液中的物质的浓度。最终滴度通常以g/L为单位表示。
如本文所用，“初始滴度”是指接种时存在的物质的浓度。初始滴度通常以g/L为单位表示。
如本文所用，“批次时间”是指在接种和发酵结束之间经过的时间量。批次时间通常以小时(h)为单位表示。
如本文所用，分批工艺的“发酵生产速率”是指发酵产物的最终滴度减去初始滴度(最终滴度减去初始滴度)除以批次时间。生产速率通常以克/升-小时(g L-1h-1)为单位表示。当应用于连续或半连续工艺时，“发酵生产速率”使用本领域已知的方法来确定。
如本文所用，“比生产速率”是指发酵生产速率除以发酵结束时的细胞干重。比生产速率通常以(g产物)(g细胞)-1h-1为单位表示。当应用于连续或半连续工艺时，“比生产速率”使用本领域已知的方法来确定。
如本文所用，发酵产物的“产物收率”是指以下两个数量的比率：a)在发酵过程中产生的产物(例如乙醇)的质量(分子)；b)添加到发酵过程中的碳源的质量(分母)。产物收率百分比通常以克/克(g/g)乘以100表示。应特别注意产品产物收率计算为质量比。产生的发酵产物的质量应当考虑批次结束时发酵培养基中存在的发酵产物的质量，以及在批次过程中收获的任何发酵产物的质量，减去批次开始时存在的发酵产物的质量，并进一步减去在批次过程中添加的任何发酵产物的质量。添加到批次中的碳源的质量应该包括在批次开始时发酵器中存在的所有碳源的质量，以及在批次过程中添加的任何碳源的质量。
如本文所用，“氧摄取速率”(“OUR”)是指在发酵期间消耗氧的体积速率。入口和出口氧浓度可通过废气分析测量，例如通过质谱仪测量。本领域普通技术人员可使用Bioreaction Engineering Principles第2版,2003,Kluwer Academic/PlenumPublishers,第449页,等式1(其以引用方式并入本文)中描述的直接法来计算OUR。体积OUR通常以(mmol O2)L-1h-1为单位测量。
如本文所用，“比氧摄取速率”是指在发酵期间消耗氧的比速率。其计算为OUR与所测量的细胞干重的比率。比OUR通常以mmol O2(g细胞干重)-1h-1为单位测量。
如本文所用，关于遗传组分的术语“外源的”是指遗传组分存在于微生物的修饰形式中，但不存在于特定微生物细胞的天然形式的基因组中。在一些实施方案中，外源遗传组分可以是细胞天然组分的修饰形式，它可以来源于另一种生物体，可以是来源于另一种生物体的组分的修饰形式，或者可以是合成来源的组分。例如，乳酸克鲁维酵母JENl基因在引入酿酒酵母中时是外源的。如本文所用，关于分子的术语“外源的”是指分子源自所提及生物体外部，例如，对于存在于发酵液中的生物体而言是外源的酶是指并非由发酵液中存在的生物体所产生和/或分泌的酶。
如本文所用，术语“异源的”是指来自与所提及生物体不同的来源的分子或活性。在一些实施方案中，被称为与所提及生物体异源的基因是在该生物体中未发现的基因。在一些实施方案中，被称为与所提及生物体异源的活性是一般不与该生物体相关的活性。例如，所提及生物体中的异源活性可包括在该生物体中发现的基因的过表达。在一些实施方案中，异源的可指包含在所提及生物体中发现的基因的多个拷贝，即，比通常在所提及生物体中发现的拷贝更多的拷贝。在一些实施方案中，异源基因可指在不同于所提及生物体的天然形式中发现的基因的位置的基因座中包含基因。
检查两种核酸或两种多肽的核酸或氨基酸序列将揭示比较的序列之间的序列同一性和相似性。序列比对和序列同一性的产生包括使用计算方法的全局比对和局部比对。可使用具有默认参数的BLAST(国家生物信息中心(NCBI)基本局部比对搜索工具)版本2.2.31软件进行比对。氨基酸序列之间的氨基酸序列同一性％可使用具有以下默认参数的标准蛋白质BLAST测定：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：6；查询范围内的最大匹配数：0；矩阵：BLOSUM62；空位成本：(存在：11，延伸：1)；组成调整：条件式组成分数矩阵调整；过滤器：没有选择；掩码：没有选择。核酸序列之间的核酸序列同一性％可使用具有以下默认参数的标准核苷酸BLAST测定：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：28；查询范围内的最大匹配数：0；匹配/不匹配分数：1，-2；空位成本：线性；过滤器：低复杂度区域；掩码：仅用于查找表的掩码。认为使用具有默认参数的NCBI BLAST版本2.2.31算法相对于参考序列具有XX％(例如，80％)的同一性分数的序列与参考序列至少XX％相同或等同、与参考序列具有XX％序列同一性。
如本文所用，术语“乳酸(lactate)”、“乳酸(lactic acid)”等可互换使用(除非另外明确说明)，并且可指乳酸、乳酸(lactic acid/lactate)的共轭碱或其盐。本文预期乳酸在被本文所述的生物体利用时主要以共轭碱的形式存在，但如本领域技术人员将理解的乳酸或盐形式也可由生物体呈现和/或利用。此外，“乳酸盐”可指D-乳酸、L-乳酸或它们的混合物。除非本文明确列举D-乳酸或L-乳酸，否则术语“乳酸”是指D-对映体和L-对映体的混合物。
如本文所用，术语“己糖”是指6碳糖或6碳糖的混合物。在一些实施方案中，己糖是葡萄糖或是包含葡萄糖的糖的混合物。贯穿本公开，任何明确提及葡萄糖的描述也适用于任何己糖或己糖的混合物。
如本文所用，术语“一元羧酸转运蛋白”、“单羧酸转运蛋白”、“单羧酸/质子同向转运蛋白”、“一元羧酸通透酶”、“单羧酸通透酶”、“羧酸通透酶”等可互换使用，并且是指携带单羧酸分子(诸如乳酸和丙酮酸)穿过细胞膜的转运蛋白。
贯穿本公开，本发明的各个方面可以以范围的形式呈现。应当了解，呈范围形式的描述仅仅是为了方便和简洁，不应理解为对本发明范围的硬性限制。因此，对范围的描述应当被认为具体公开了所有可能的子范围以及该范围内的各个数值。例如，对诸如1至7的范围的描述应被认为具体公开了诸如1至3、1至4、1至6、2至5、3至5等子范围，以及该范围内的各个数字，例如1、2、3、3.6、4、5、5.8、6、7和其间的任何整数及分数增量。不管范围有多宽，这都适用。
描述
本文描述了经遗传修饰的生物体、用于产生此类生物体的方法以及使用此类生物体的发酵工艺。生物体在发酵工艺期间可消耗乳酸，同时产生生物产物。在一个方面，生物体可在葡萄糖存在下消耗乳酸，同时产生生物产物。因此，本发明涉及代替碳水化合物或除碳水化合物之外可使用乳酸作为发酵碳源的生物体和工艺。因此，这些生物体和工艺可用于使含有乳酸的底物，诸如玉米或其他植物加工物流发酵。
许多发酵底物进料物流，包括玉米工艺物流，含有一定量的乳酸。例如，在湿玉米碾磨工艺的浸泡步骤中产生乳酸的乳酸杆菌(Lactobacilli)的生长通常得到促进，帮助玉米分解成其各种组分。然而，在当前可用的发酵工艺中，发酵液中的大部分或全部乳酸仍未被消耗。因此，本发明的工艺和生物体可提供优于当前可用的工艺或生物体的益处，方式为消耗进料物流中更高百分比的可用碳源，从而使发酵收率、生产速率和/或效率得到改善。
经遗传修饰的酵母及用于产生此类酵母的方法
在一个方面，本发明涉及经遗传修饰的酵母及用于产生此类酵母的方法。在一些实施方案中，经遗传修饰的酵母属于酵母属。在一些实施方案中，酵母是经遗传修饰的酿酒酵母。在一个方面，酵母经遗传修饰以包括一种或多种性状，所述一种或多种使酵母能够消耗乳酸或改善酵母的乳酸消耗能力，同时产生发酵产物。在一些实施方案中，相比没有本文所述的修饰的酵母，所述酵母可以更快的速率消耗乳酸，特别是当发酵液中存在己糖或己糖低聚物诸如葡萄糖时。在一些实施方案中，相比没有本文所述的修饰的酵母，所述酵母可以更快的速率消耗D-乳酸、L-乳酸或D-乳酸和L-乳酸两者。在一些实施方案中，相比没有本文所述的修饰的酵母，所述酵母可以更高的滴度产生生物产物和/或以更快的速率产生生物产物。在一些实施方案中，生物产物是乙醇。
遗传修饰可包括转化酵母以包含与乳酸消耗或利用相关的一种或多种异源基因。遗传修饰的非限制性实例包括：转化酵母以过表达一种或多种天然型基因；转化酵母以包括一个或多个天然型基因的多个拷贝；用一种或多种外源基因转化酵母；和/或转化酵母以包括一个或多个外源基因的多个拷贝。
在一个方面，遗传修饰可涉及乳酸转运，例如转化酵母以表达异源单羧酸/质子同向转运蛋白基因。在一些实施方案中，单羧酸/质子同向转运蛋白基因来自克鲁维酵母属的酵母。在一些实施方案中，单羧酸/质子同向转运蛋白基因是乳酸克鲁维酵母(SEQ ID NO:27)、马克斯克鲁维酵母(SEQ ID NO:73)或多布克鲁维酵母(SEQ ID NO:71)JEN1同向转运蛋白基因。在一些实施方案中，单羧酸/质子同向转运蛋白基因来自耶氏酵母属的酵母，例如解脂耶氏酵母(Yarrowia lypolitica)(SEQ ID NO:79)。在一些实施方案中，单羧酸/质子同向转运蛋白基因来自除克鲁维酵母属或耶氏酵母属以外的酵母属，例如但不限于酵母属或伊萨酵母属。在一些实施方案中，单羧酸/质子同向转运蛋白基因来自酿酒酵母或东方伊萨酵母(SEQ ID NO:23、SEQ ID NO:25)。在一些实施方案中，经遗传修饰的酵母编码多肽，所述多肽具有与以下氨基酸序列中的至少一者具有至少65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:24、SEQ ID NO:26、SEQ IDNO:28、SEQ ID NO:76或SEQ ID NO:78。在一些实施方案中，酵母编码多肽，所述多肽具有与乳酸克鲁维酵母(SEQ ID NO:28)或多布克鲁维酵母(SEQ ID NO:76)JEN1多肽或两者具有至少70％、74％或75％的序列同一性的氨基酸序列。
在一些实施方案中，遗传修饰可涉及乳酸消耗或利用。在一些实施方案中，转化酵母以表达一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(EC 1.1.2.3或1.1.2.4)。在一些实施方案中，转化酵母以表达异源细胞色素b2(CYB2)基因。在一些实施方案中，CYB2基因是酿酒酵母(SEQ ID NO:13)或东方伊萨酵母CYB2(SEQ ID NO:16)基因。在一些实施方案中，CYB2基因来自克鲁弗酵母(SEQ ID NO:53)、贝酵母(SEQ ID NO:55)、鲁氏接合酵母(SEQID NO:57)、乳酸克鲁维酵母(SEQ ID NO:59)或多布克鲁维酵母(SEQ ID NO:61)。在一些实施方案中，具有异源CYB2基因的经遗传修饰的酵母编码多肽，所述多肽具有与以下氨基酸序列中的至少一者具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:15、SEQ ID NO:17、SEQ ID NO:54、SEQID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62。
在一些实施方案中，转化酵母以表达异源D-乳酸脱氢酶(DLD)基因。在一些实施方案中，DLD基因是酿酒酵母(SEQ ID NO:39)或东方伊萨酵母DLD(SEQ ID NO:41)基因。在一些实施方案中，DLD基因来自克鲁弗酵母(SEQ ID NO:63)、贝酵母(SEQ ID NO:65)、烟曲霉(SEQ ID NO:67)、乳酸克鲁维酵母(SEQ ID NO:69)、多布克鲁维酵母(SEQ ID NO:71)或马克斯克鲁维酵母(SEQ ID NO:73)。在一些实施方案中，具有异源DLD基因的经遗传修饰的酵母编码多肽，所述多肽具有与以下氨基酸序列中的至少一者具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ IDNO:40、SEQ ID NO:42、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ IDNO:72或SEQ ID NO:74。在一些实施方案中，转化酵母以表达异源CYB2基因和DLD基因两者。
在一个方面，可转化经遗传修饰的酵母以包括异源单羧酸/质子同向转运蛋白基因和乳酸脱氢酶基因的任何组合。在一些实施方案中，酵母含有异源单羧酸/质子同向转运蛋白基因、异源CYB2基因和异源DLD基因。在一些实施方案中，酵母含有异源单羧酸/质子同向转运蛋白基因和异源CYB2基因。在一些实施方案中，酵母含有异源单羧酸/质子同向转运蛋白基因和异源DLD基因。在一些实施方案中，酵母含有异源单羧酸/质子同向转运蛋白基因、异源CYB2基因和/或异源DLD基因中任一者的多于一个拷贝，即，酵母可用异源单羧酸/质子同向转运基因的两个或更多个拷贝；异源CYB2基因的两个或更多个拷贝；和/或异源DLD基因的两个或更多个拷贝转化。此外，可修饰酵母以含有来自任何物种组合的异源基因，例如，可修饰酿酒酵母以含有乳酸克鲁维酵母JEN1的一个或多个拷贝、异源酿酒酵母CYB2的一个或多个拷贝、以及异源酿酒酵母DLD的一个或多个拷贝。因此，酵母可编码多肽，所述多肽具有与单羧酸/质子同向转运蛋白相关的氨基酸序列、CYB2相关的氨基酸序列和/或DLD相关的氨基酸序列中的一者、二者、三者或更多者，例如SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:76、SEQ ID NO:78、SEQ ID NO:15、SEQ ID NO:17、SEQ IDNO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60、SEQ ID NO:62、SEQ ID NO:40、SEQ IDNO:42、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ ID NO:72或SEQID NO:74中的至少一者具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列。
尽管不希望受理论束缚，但本文预期生物体所消耗的乳酸主要转变为丙酮酸，并且至少一部分丙酮酸转变为所需的发酵产物。甚至被生物体消耗用于呼吸的乳酸也可提供益处，因为生物体使用乳酸而不是其他可能优选的碳源如葡萄糖。
可消耗D-乳酸并产生乙醇的酿酒酵母菌株已得到描述(Wakamatsu等人,2013,Biosci.Biotechnol.Biochem.77(5)1114-1116)。然而，Wakamatsu中描述的菌株是从仅含有乳酸的发酵培养基中产生乙醇，而不是从还含有葡萄糖的培养基中产生乙醇。此外，Andrade描述了JEN1表达因葡萄糖存在而受到抑制(Andrade等人,2001,Fungal Geneticsand Biology 32,105-111)。因此，当前可获得的酵母菌株不能同时消耗大量的乳酸和葡萄糖，同时以商业上有用的速率产生发酵产物。此外，已知许多酵母在发酵期间产生一些乳酸而不是消耗乳酸。然而，本发明涉及的经遗传修饰的酵母可令人惊讶地同时使用乳酸和葡萄糖作为碳源，以适合商业应用的速率产生发酵产物。在一个方面，相比当前可获得的酵母，经遗传修饰的酵母可以显著更高的速率消耗L-乳酸，无论是否存在葡萄糖或另一种己糖。
在一个方面，可对酵母进行遗传修饰以消耗D-乳酸、L-乳酸或D-乳酸和L-乳酸的混合物。含有乳酸的植物加工物流通常含有D-乳酸和L-乳酸对映体的混合物，例如外消旋混合物或含有除50:50之外的一些比例的D-酸和L-乳酸的混合物。在一个方面，可对酵母进行遗传修饰以含有表达异源乳酸消旋酶的基因。含有表达消旋酶的基因的酵母可用于改善酵母的乳酸消耗特征。例如，可对根据本发明的相比消耗L-乳酸更快地消耗D-乳酸的经遗传修饰的酵母进行修饰以表达乳酸消旋酶。由于D-乳酸被酵母更快地消耗，所以消旋酶可用于将L-乳酸转变为D-乳酸以改善乳酸消耗的总体速率。
如上所述，酵母可包含一种或多种异源JEN1、DLD和/或CYB2基因，导致编码与本文列出的某些氨基酸序列具有最小序列同一性的多肽。在一些实施方案中，已经鉴定了对应于特定残基范围的位置处的标签模式。在一些实施方案中，用具有这些保守模式的构建体转化本发明的酵母。因此，在一些实施方案中，酵母可编码在某些氨基酸残基位置处具有标签模式的多肽。
在一个方面，经遗传修饰的酵母编码CYB2蛋白，所述CYB2蛋白具有在指定位置保守的以下氨基酸残基：Lys349、Tyrl43、Tyr254和His373。Xia描述了这些残基在乳酸催化氧化成丙酮酸中的作用(Xia和Mathews,1990,J.of Molecular Biology.212第837-863页，其以引用方式整体并入本文)。在一些实施方案中，本发明的酵母编码与SEQ ID NO:15、SEQID NO:17、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％或95％的序列同一性的多肽，所述多肽在指定位置包括以下氨基酸残基中的一者或多者：Lys349、Tyrl43、Tyr254和His373。氨基酸Lys349、Tyrl43、Tyr254和His373通过与丙酮酸相互作用在乳酸氧化机制中起作用。在一些实施方案中，这种酵母包含来自以下任何物种的异源CYB2基因：贝酵母、克鲁弗酵母、鲁氏接合酵母、乳酸克鲁维酵母、多布克鲁维酵母、马克斯克鲁维酵母、东方伊萨酵母、烟曲霉或黑曲霉(A.niger)。在一些实施方案中，这种酵母还包含至少一种异源单羧酸/质子同向转运蛋白基因，并编码与SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:76或SEQ ID NO:78具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或99％的序列同一性的多肽。在一些实施方案中，这种酵母还包含至少一种异源DLD基因并编码与SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:64、SEQ ID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ ID NO:72或SEQ ID NO:74具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或99％的序列同一性的多肽。
在一个方面，相比当前可获得的酵母，经遗传修饰的酵母可以更快的速率消耗D-乳酸、L-乳酸或它们的混合物。在一些实施方案中，在当前可获得的酵母不能消耗D-乳酸和/或L-乳酸的条件下，经遗传修饰的酵母可消耗D-乳酸和/或L-乳酸。在一个方面，根据本文稍后描述的乳酸消耗评估方案测量乳酸消耗速率是有用的。在一些实施方案中，当己糖浓度为150g/L或更高时，酵母具有至少0.030gL^h-1的L-乳酸消耗速率。在一些实施方案中，当己糖浓度为150g/L或更高时，酵母具有至少0.035、0.040、0.045或0.050gL^h-1的L-乳酸消耗速率。在一些实施方案中，当己糖浓度为5g/L或更高时，酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的L-乳酸消耗速率。在一些实施方案中，当己糖浓度为150g/L或更高时，酵母具有至少0.005、0.006、0.007、0.008、0.009、0.010、0.015、0.020、0.025或0.030gL^h-1的D-乳酸消耗速率。在一些实施方案中，当己糖浓度为5g/L或更高时，酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的D-乳酸消耗速率。
在一些实施方案中，酵母可包括其他遗传修饰。在一个方面，可转化酵母以表达可用于改善生物产物生产的酶。在一个方面，可转化酵母以含有与表达通常添加到发酵工艺中的酶(例如用于从己糖低聚物产生葡萄糖或另一种己糖的酶)相关的基因。在一个方面，可转化酵母以减少或消除碳源向不需要的化合物的转变。在一些实施方案中，酵母可包括可用于改善乙醇生产工艺的任何其他方面的一种或多种遗传修饰。可用于产乙醇酵母的遗传修饰的非限制性实例包括转化酵母以表达用于产生葡糖淀粉酶的异源基因(EC3.2.1.3)，例如酵母表达来自扣囊复膜酵母或米根霉的葡糖淀粉酶；转化酵母以表达用于消耗异麦芽糖的异源基因；和/或转化酵母以减少发酵期间酵母中甘油的形成。在一些实施方案中，可转化酵母以分泌异源乳酸氧化酶。此外，可转化酵母以除了乳酸氧化酶之外分泌异源过氧化氢酶以消耗过氧化氢。
在一些实施方案中，对酵母进行的与增加乳酸消耗相关的遗传修饰(例如，修饰酵母以包含异源CYB2基因、DLD基因和/或JENl基因)还可涉及发酵期间由酵母产生的甘油的减少。发酵期间产生的甘油的减少可增加从一种或多种碳源(例如，葡萄糖和/或乳酸)转化为发酵产物的碳的量。因此，相比不包含与增加的乳酸消耗相关的遗传修饰的酵母，所述酵母可产生更高收率的发酵产物(诸如乙醇)，并产生更低量的甘油。
在一些实施方案中，可通过除遗传修饰之外的方法对酵母进一步工程化。在一些实施方案中，在如上所述对酵母进行遗传修饰后，使酵母进化。在一些这样的实施方案中，进化可包括将酵母繁殖多代以允许酵母自然地选择某些特征。
在一些实施方案中，可通过诱变对酵母进行修饰。在一些这样的实施方案中，可在诱变后选择酵母的某些特征。在一些实施方案中，诱变可包括使酵母经历紫外线辐射和/或化学处理。在一些实施方案中，酵母的选定特征可包括热耐受性、乙醇耐受性、抑制剂耐受性、改善的生长速率和/或副产物减少。
因此，在一个方面，本发明还涉及一种用于进化可以消耗乳酸同时产生发酵产物的酵母的方法。在一些实施方案中，所述方法包括以下步骤：转化酵母以表达与增加的乳酸消耗相关的一种或多种异源基因，然后进化酵母并基于所需特征选择酵母。在一些实施方案中，所述方法包括以下步骤：转化酵母以表达与增加的乳酸消耗相关的一种或多种异源基因，使酵母暴露于能够引起诱变的环境，并基于一种或多种所需特征选择酵母。在一个方面，用于进化酵母的方法可使用本文所述的任何酵母，即经转化以增加发酵期间的乳酸消耗的经遗传修饰的酵母。
在一个方面，本发明的非天然酵母能够在比通常在发酵工艺中使用酵母(例如酿酒酵母)时的温度更高的温度下生长和/或产生发酵产物。例如，酿酒酵母通常在约30℃的温度下具有最佳生长。在一些实施方案中，酵母对诸如32℃或更高，诸如在高于32℃至约40℃范围内的升高温度具有更大的耐受性。升高温度的示例性范围是Ti至至T2，其中Ti选自32.2℃、32.4℃、32.6℃、32.8℃、33℃、33.2℃、33.3℃、33.4℃、33.6℃、33.8℃、34℃、34.2℃、34.4℃、34.6℃、34.8℃、35℃和36℃；并且T2选自36℃、37℃、38℃、39℃和40℃。出于本公开的目的，如果酵母在暴露于具有升高温度的发酵培养基期间或之后可继续生长、繁殖和/或产生发酵产物，则认为酵母是“耐热的”。
在发酵工艺期间，发酵培养基在发酵工艺期间的一个或多个时间期间可达到32℃或更高的升高温度。在部分发酵期期间或整个发酵期期间，可升高温度。可升高温度持续5分钟更长、10分钟更长、30分钟或更长、1小时或更长、2小时或更长、5小时或更长或10小时或更长。升高温度的时间也可表示为占总发酵期的总和，诸如占发酵期的约0.1％至100％、约0.1％至约75％、约0.1％至约50％、约0.1％至约25％、约0.1％至约10％、约0.1％至约5％、约0.1％至约2.5％、约0.1％至约1％或约0.1％至约0.5％。
工程化酵母还可在升高温度期期间或之后提供商业上相关的乙醇滴度。例如，在升高温度期(例如对应于Ti至T2的任何范围)期间或之后，乙醇滴度可在约l10g/L至约170g/L的范围内、在约125g/L至约170g/L的范围内或在约140g/L至约170g/L的范围内。因此，本文所述的工程化酵母可在高温期期间或之后以商业上有用的滴度产生乙醇，而在当前可用于乙醇生产发酵工艺的酵母菌株中所述高温期通常会引起问题。这些问题包括但不限于：相当大百分比的酵母细胞死亡；对酵母繁殖能力造成有害影响；和/或减少或消除酵母产生发酵产物的能力。
可将本文所述的工程化酿酒酵母置于温度选择压力下，以选择对在较高温度下生长具有增加的耐受性的菌株。在施加较高温度选择之前，可对工程化酵母进行随机诱变(例如UV、化学)，以产生可赋予改善的对在这些较高温度下生长的耐受性的突变。例如，本公开的工程化酵母可在32℃或更高的温度下具有比参考酵母的生长速率高10％、20％、30％、40％或50％的比生长速率。在一些实施方案中，参考酵母是表达扣囊复膜酵母葡萄糖淀粉酶的酵母，该酵母能够以至少90g/L的滴度产生乙醇。在一个方面，本公开还涉及一种用于产生适用于发酵工艺的酵母产品的方法。在一些实施方案中，这种方法包括以下步骤：培育酵母(例如本文所述的任何工程化酵母)；以及从用于培育酵母的培养基中分离酵母。所述方法可还包括干燥酵母以形成酵母产品的步骤。干燥酵母可改善酵母的保质期。在一些实施方案中，干酵母的水分含量小于5％、小于4％、小于3％、小于2％或小于1％。
发酵工艺
在一个方面，本发明涉及发酵工艺。在一个方面，发酵工艺可以是使用本文所述的经遗传修饰的酵母的实施方案产生发酵产物的任何工艺。在一些实施方案中，发酵产物是乙醇。在一些实施方案中，发酵产物可以是除乙醇之外的醇，例如但不限于正丙醇、异丙醇、正丁醇、异丁醇、丁二烯或异戊二烯。
示例性发酵工艺可包括以下步骤：提供含有碳源的发酵培养基；向发酵培养基中添加酵母；用酵母使培养基发酵以产生生物产物；以及收获生物产物。在一个方面，培养基中的碳源可包括淀粉、糖、有机酸或它们的混合物。在一些实施方案中，培养基中的碳源是葡萄糖低聚物、葡萄糖和/或乳酸的混合物。
在一个方面，含乳酸的植物工艺物流用作发酵培养基或在发酵工艺中的某一时刻添加到发酵培养基中。在一个方面，本发明的工艺可用于使除葡萄糖和/或葡萄糖低聚物之外还含有大量乳酸的发酵培养基发酵。含有大量乳酸的发酵培养基的非限制性实例包括具有至少0.1、至少0.5、至少1、至少2、至少3、至少4或至少5g/L乳酸的培养基。在一些实施方案中，在发酵开始时发酵培养基可具有在约1-20、2-20、3-20、4-20、5-20、1-15、3-15或5-15g/L范围内的乳酸含量。在一些实施方案中，在发酵开始时发酵培养基可具有约1-50、15-30或20-30g/L的乳酸含量。例如，在一些实施方案中，轻质浸泡水(LSW)可含有约25g/L乳酸。
在一些实施方案中，本文所述的工艺可用于消耗发酵培养基中的大部分或全部乳酸。在一些实施方案中，所述工艺可仅用于消耗发酵培养基中的一部分乳酸。所述工艺期间的乳酸消耗可取决于许多因素，包括但不限于：所用酵母的特征；向工艺中添加氧的速率；发酵开始时存在的氧量；发酵培养基中的葡萄糖浓度；pH以及温度。
在一个方面，如本领域技术人员将理解的，培养基的组成可在发酵期间变化。例如，葡萄糖或另一种己糖可在发酵期间通过酶活性从低聚物产生，然后被消耗。因此，在一些实施方案中，如果酵母消耗葡萄糖的速率高于葡萄糖低聚物产生葡萄糖的速率，则在发酵的某些时刻葡萄糖含量可能非常低或甚至不可检测。在一些实施方案中，例如补料分批发酵中，培养基可连续或半连续地补充有进料物流，诸如植物工艺进料物流。在一些实施方案中，由于酵母的消耗，在发酵的某些点时刻乳酸含量可能非常低或甚至不可检测，那么就可通过植物工艺进料物流将更多的乳酸添加到培养基中。
因此，在一个方面，用于本文所述工艺的发酵培养基的各种组分的浓度可以是平均浓度。组分的平均浓度可通过本领域已知的方法来计算，例如通过取发酵开始时发酵培养基中组分的浓度和发酵结束时发酵培养基中相同组分的浓度的平均值来计算。这种平均值的计算还可将发酵工艺期间任何输入物流和/或输出物流中组分的浓度考虑在内。此外，对于连续发酵工艺，组分的平均浓度可指任何单个容器中的平均浓度，或者它可指整个工艺中的平均浓度，即，将工艺中所有进料物流和所有输出物流考虑在内。
在一些实施方案中，至少5％、10％、15％、20％、25％、30％、35％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或99％的存在于发酵培养基中和/或添加到发酵培养基中的乳酸在发酵结束时被消耗。在一些实施方案中，发酵期间的乳酸消耗速率在0.01至1.5g L-1h-1、0.05至1g L-1h-1或0.1至1g L-1h-1的范围内。在一些实施方案中，发酵期间的乳酸消耗速率为至少0.01g L-1h-1、0.05g L-1h-1、0.1g L-1h-1、0.5gL-1h-1或1g L-1h-1。在一些实施方案中，上文列出的乳酸消耗速率可指发酵期间的平均乳酸消耗。在一些实施方案中，上文列出的乳酸消耗速率可指发酵工艺的第一部分期间的乳酸消耗，例如在发酵工艺的前10小时、前8小时、前6小时、前4小时、前2小时、前1小时、或前30分钟内的乳酸消耗。在一些实施方案中，上文列出的乳酸消耗速率是指存在葡萄糖时的乳酸消耗速率。在一些实施方案中，当发酵期间的乳酸消耗速率为至少0.01g L-1h-1、0.05g L-1h-1、0.1g L-1h-1、0.5g L-1h-1或1g L-1h-1时，葡萄糖浓度为至少0.1g/L、1g/L、10g/L、20g/L或30g/L。
在一些实施方案中，发酵结束时发酵培养基中总乳酸的量在0至3g/L、0至1g/L、0至0.5g/L、0至0.1g/L、0.001至3g/L、0.001至1g/L或0.001至0.1g/L的范围内。在一些实施方案中，发酵结束时发酵培养基中的总乳酸的量小于3g/L、2g/L、1g/L、0.5g/L或0.1g/L。在一些实施方案中，发酵结束时发酵培养基中L-乳酸的量在0至3g/L、0至1g/L、0至0.5g/L、0至0.1g/L、0.001至1g/L或0.001至0.1g/L的范围内。在一些实施方案中，发酵结束时发酵培养基中的L-乳酸的量小于3g/L、2g/L、1g/L、0.5g/L或0.1g/L。在一些实施方案中，发酵结束时发酵培养基中D-乳酸的量在0至3g/L、0至1g/L、0至0.5g/L、0至0.1g/L、0.001至1g/L或0.001至0.1g/L的范围内。在一些实施方案中，发酵结束时发酵培养基中的D-乳酸的量小于3g/L、2g/L、1g/L、0.5g/L或0.1g/L。
在一个方面，本发明的工艺可用于以商业上显著的速率和/或滴度产生乙醇。在一些实施方案中，产生乙醇的速率可以是1至6g L-1h-1、1至5.5g L-1h-1或1至5g L-1h-1。在一些实施方案中，发酵结束时的乙醇滴度为至少90g/L、100g/L、110g/L、120g/L、125g/L、130g/L、131g/L、132g/L、133g/L、134g/L或至少135g/L。在一些实施方案中，发酵结束时的乙醇滴度在120g/L至155g/L、130至155g/L、135至155g/L、130至150g/L或130至140g/L的范围内。
在一个方面，所述工艺可使用除本文所述的经遗传修饰的酵母之外的酵母，例如可商购获得的ETHANOL REDTM酵母(可从Lesaffre/Fermentis获得)，并且所述工艺可使用高氧摄取速率和/或较低葡萄糖浓度来促进乳酸消耗。
在一个方面，所述工艺可用于消耗L-乳酸、D-乳酸或L-乳酸和D-乳酸的混合物。在一个方面，所述工艺可包括使用乳酸消旋酶来改善酵母的乳酸消耗特征。在一些实施方案中，所述工艺中使用的酵母可以是分泌乳酸消旋酶的经遗传修饰的酵母。在一些实施方案中，可代替由酵母分泌的乳酸消旋酶，或者在由酵母分泌的乳酸消旋酶之外，将外源乳酸消旋酶添加到工艺中。可将其他外源酶添加到所述工艺中。例如，在一些实施方案中，可将外源乳酸氧化酶添加到所述工艺中。在一个这样的实施方案中，可将外源过氧化氢酶添加到所述工艺中以分解由外源乳酸氧化酶的活性形成的过氧化氢。
分批发酵工艺
在一个方面，本发明的工艺可以是分批发酵工艺。在一些实施方案中，本发明的分批工艺是干研磨或干碾磨乙醇生产工艺。分批发酵工艺，包括干研磨乙醇工艺，在本领域中是熟知的。如本领域技术人员将理解的，在玉米或其他植物物质的干碾磨期间，例如由于细菌污染可形成乳酸。在一个方面，本发明的经遗传修饰的酵母可用于分批发酵工艺以使用乳酸作为碳源来产生发酵产物。
示例性分批发酵工艺包括以下步骤：提供含有乳酸和/或其他碳源诸如碳水化合物的发酵培养基，以及使用本文所述类型的经遗传修饰的酵母使培养基发酵。在一些实施方案中，酵母含有异源单羧酸/质子同向转运蛋白(例如JENl)基因。在一些实施方案中，在发酵开始时培养基含有浓度为至少0.5、1、2或3g/L的葡萄糖或葡萄糖低聚物。在一些实施方案中，在发酵开始时乳酸浓度为至少0.5、1、2、3、4、5或6g/L。在一些实施方案中，在发酵开始时乳酸浓度在1至20g/L、1至15g/L、1至10g/L、1至8g/L或1至5g/L的范围内。在一些实施方案中，在发酵结束时总乳酸的至少20％、30％、40％、50％、60％、70％、80％、90％、95％或99％被消耗。在一些实施方案中，在发酵结束时D-乳酸的至少50％、60％、70％、80％、90％、95％或99％被消耗。在一些实施方案中，在发酵结束时L-乳酸的至少50％、60％、70％、80％、90％、95％或99％被消耗。
在一个方面，对于分批发酵工艺的至少一部分，以最小体积氧摄取速率(OUR)运行所述工艺。在一些实施方案中，在所述工艺的至少一部分期间，OUR为至少0.5、1.0或1.5mmol 0 2/(L-h)。在一些实施方案中，在所述工艺的至少一部分期间，OUR在0.5至5.0mmol 0 2/(L-h)的范围内。在一些实施方案中，在所述工艺的至少一部分期间，OUR在3.5至4.5mmol 0 2/(L-h)的范围内。在一些实施方案中，在所述工艺的初始部分内，OUR为至少0.5、1.0或1.5mmol 0 2/(L-h)。在一些实施方案中，在所述工艺的前6、8或10小时内，OUR为至少约0.5、或1.0或1.5mmol 02/(L-h)。在一些实施例中，在所述工艺的部分期间，OUR降低或大约为零。在一些实施方案中，在所述工艺的约前6、8、10、12、15或20小时后，OUR小于0.5或1.0mmol 02/(L-h)。
连续发酵工艺
在一个方面，本发明的工艺可以是连续或半连续发酵工艺。在一些实施方案中，本发明的连续工艺是湿玉米研磨乙醇生产工艺。连续发酵工艺，包括湿碾磨乙醇工艺，在本领域中是熟知的。在一些实施方案中，具有连续操作模式的发酵工艺包括串联操作的多个发酵器，其中在第一发酵器中供应淀粉水解产物，所述淀粉水解产物供给第二发酵器以此类推，直至淀粉水解产物转化为乙醇。在一些实施方案中，可使用1至10或2至7个发酵器操作连续操作。在一些实施方案中，可在单个容器中执行连续发酵工艺，其中可以以连续或半连续的方案添加原料并移出含有产物的发酵液。
用于制造乙醇的示例性连续发酵工艺包括以下步骤：提供含有葡萄糖或葡萄糖低聚物的初始发酵培养基，用经遗传修饰的酵母发酵发酵培养基，向发酵培养基中添加一种或多种包含乳酸的进料物流，并去除至少一个输出物流包含来自发酵培养基的乙醇。在一些实施方案中，除葡萄糖或葡萄糖低聚物外，初始发酵培养基可含有乳酸盐和/或其他碳源。在一个方面，经遗传修饰的酵母是根据本文所述的经遗传修饰的酵母的任何实施方案的酵母。在一些实施方案中，酵母含有异源单羧酸/质子同向转运蛋白(例如JEN1)基因。
在一些实施方案中，将初始发酵培养基添加至发酵器或生长发酵器容器中，其中添加并培育经遗传修饰的酵母直至获得所需的生物质。在一些实施方案中，将预发酵器中的工艺条件设定为有利于细胞生长而不是发酵产物形成。在一些实施方案中，然后可将预发酵器容器的内容物转移到第二发酵器容器中。在第二发酵器容器中，该过程的条件设定为有利于形成发酵产物而不是细胞生长。在一些实施方案中，将额外的发酵培养基以单一部分或以连续或半连续方式添加到第二发酵器容器中。在一些实施方案中，添加到第二发酵器容器中的另外的发酵培养基含有乳酸和/或其他碳源。上文提及的第二发酵器也可称为“繁殖器”。在一些实施方案中，可将第二发酵器容器的内容物转移到第三发酵器容器中。第三发酵器容器的工艺条件可与第二发酵器容器相同或不同。在一些实施方案中，如连续发酵工艺领域的技术人员将理解的，可将第三发酵器容器的内容物转移到一个或多个另外的发酵器容器中。在一些实施方案中，从最终发酵器容器的内容物中分离生物产物，例如乙醇。
在一些实施方案中，预发酵器容器中的发酵培养基的平均葡萄糖浓度在10至20g/L的范围内。在一些实施方案中，第二发酵器容器中的发酵培养基的平均葡萄糖浓度在30至40g/L的范围内。在一些实施方案中，第三发酵器容器或任何另外的发酵器容器中的发酵培养基的平均葡萄糖浓度在30至40g/L的范围内。在一些实施方案中，最终发酵器容器中的发酵培养基的平均葡萄糖浓度在0至5g/L的范围内。在一些实施方案中，预发酵器容器、繁殖器容器或任何发酵容器中的发酵培养基的平均葡萄糖浓度在0-5、2-5、1-10、5-10、5-15、5-20、10-20、15-25、20-30、25-35、30-40或35-45g/L的范围内。在一些实施方案中，预发酵器容器、繁殖器容器或任何发酵容器中的发酵的平均葡萄糖浓度维持在大于或等于与酵母中葡萄糖抑制相关的葡萄糖浓度的范围内。在一些实施方案中，与酵母中葡萄糖抑制相关的葡萄糖浓度在2至5g/L的范围内。因此，在这样的实施方案中，预发酵器容器或任何发酵容器中的发酵培养基的平均葡萄糖浓度可维持在大于或等于2、3、4或5g/L的水平。
可在连续发酵工艺中调整和/或维持其他发酵条件，包括但不限于：温度、pH、体积或比氧摄取速率(OUR)，或任何碳源或任何发酵培养基营养物的浓度。在一些实施方案中，预发酵器容器、繁殖器容器或任何其他发酵容器中的温度可在20℃-45℃、20℃-40℃、20℃-30℃、25℃-35或30-40℃的范围内。在一些实施方案中，预发酵器容器、繁殖器容器或任何其他发酵容器中的pH可在2至7、3至6、4.5至5.5或3.5至4.5的范围内。如本领域技术人员将理解的，由于乳酸被酵母消耗，发酵开始时的pH可低于发酵结束时的pH。因此，发酵结束时的pH可处于与发酵开始时的pH不同的上述pH范围内。在一些实施方案中，预发酵器容器、繁殖器容器或任何其他发酵容器中的体积OUR为至少0.5mmol 02/(L-h)或至少1mmol 02/(L-h)。在一些实施方案中，预发酵器容器、繁殖器容器或任何其他发酵容器中的体积OUR在0.1至5mmol 02/(L-h)、3至5mmol 02/(L-h)、0.2至1mmol 02/(L-h)、0.4至0.6mmol 02/(L-h)、4至200mmol 02/(L-h)、8至10mmol 02/(L-h)或4至30mmol 02/(L-h)的范围内。在一些实施方案中，预发酵器容器、繁殖器容器或任何其他发酵容器中的体积OUR为至少4mmol 02/(L-h)、至少5mmol 02/(L-h)、至少6mmol 02/(L-h)、至少7mmol 02/(L-h)或至少8mmol O^L-h)。
在一些实施方案中，预发酵器容器中的细胞密度在3至10g/L或5至10g/L的范围内。在一些实施方案中，繁殖器容器中的细胞密度在10至50g/L的范围内
在一个方面，如本文所述，酵母在发酵工艺期间消耗乳酸。在一些实施方案中，所有输出物流的总和中的总乳酸含量小于添加到发酵工艺中的乳酸(即，所有输入或进料物流的总和中的乳酸含量)的90％。在一些实施方案中，发酵工艺的输出中的总乳酸含量小于发酵工艺的输入的99％、95％、85％、80％、75％、70％、65％、60％、55％、50％、45％、40％、35％、30％、25％、20％、15％或10％。在一些实施方案中，发酵工艺的输出中的D-乳酸含量小于发酵工艺的输入的99％、95％、90％、85％、80％、75％、70％、65％、60％、55％、50％、45％、40％、35％、30％、25％、20％、15％或10％。在一些实施方案中，发酵工艺的输出中的L-乳酸含量小于发酵工艺的输入的99％、95％、90％、85％、80％、75％、70％、65％、60％、55％、50％、45％、40％、35％、30％、25％、20％、15％或10％。
本文所述的连续发酵工艺可以商业上显著的速率生产乙醇或另一种生物产物。在一些实施方案中，所述工艺可以至少0.5、1.0、1.5、2.0、2.5、3.0或3.2g L-1h-1的速率生产生物产物。
生产某些生物产物方面的优点
在一个方面，酵母的乳酸消耗特征可在生产某些生物产物方面或在某些发酵工艺方面提供优势。本文所述的经遗传修饰的酵母和发酵工艺可用于制备乙醇或其他感兴趣的生物产物。其他生物产物包括除乙醇之外的醇、乳酸、3-羟基丙酸(3-HP)和琥珀酸。
在一些实施方案中，本文所述的酵母可用于制备对映体纯的乳酸。例如，通过消耗一种具有相对高特异性的对映体，酵母可产生具有大于95％、97％、98％、99％、99.5％或更高的对映体纯度的D-乳酸或L-乳酸。
在一些实施方案中，酵母可改善有机酸诸如琥珀酸或3-HP的产生。例如，当在下游加工中从发酵液中回收有机酸产物时，从有机酸产物中分离乳酸可能是成问题的。通过消耗乳酸，本文所述的酵母可提高下游加工的效率和/或提高最终有机酸产物的纯度。
在一个方面，本文所述的酵母可使植物工艺物流能够在通常不使用这些流的发酵工艺中使用。植物工艺物流(诸如玉米浆)通常不用于生产除乙醇之外的生物产物，因为在这些物流中存在诸如乳酸的杂质。通过消耗乳酸，本发明的酵母可使植物工艺物流能够在各种各样的发酵工艺中使用。
实验实施例
通过参考以下实验实施例进一步详细地描述本发明。除非另外指明，否则提供这些实施例仅仅是为了说明的目的，并非旨在为限制性。因此，决不应将本发明理解为限于以下实施例，相反，应理解为涵盖由于本文提供的教导而变得显而易见的任何及所有变型。
乳酸消耗评估方案
下述方案用于确定酵母菌株对L-乳酸、D-乳酸或D-乳酸和L-乳酸的混合物(即“总乳酸”)的消耗。
选择酵母菌株并在ScD-Ura平板上划线，然后在30℃下孵育直至可见单菌落(1-2天)或者在30℃/250rpm下在14ml培养管中作为液体培养物的4ml YP+100g/L D-葡萄糖中生长过夜以产生种子生物质。用种子细胞浆液接种摇瓶以达到0.1-0.3的初始ODδoo。在即将接种之前，将60mL摇瓶培养基添加到250mL的无挡板摇瓶(Corning 4995-250)中，该摇瓶装有含透气密封的螺旋盖(corning 1395-45LTMC)。摇瓶培养基根据Verduyn(Verduyn等人,1992,Yeast.第8,501-517卷，其以引用方式整体并入本文)制备，不同之处在于用二水合锰代替四水合锰。向培养基补充D-葡萄糖以达到初始D-葡萄糖浓度。例如，向培养基补充250克D-葡萄糖/升培养基。要仅评估L-乳酸消耗，使用L-乳酸钠(例如，Sigma；目录号L7022)。要仅评估D-乳酸消耗，使用D-乳酸钠(例如，Sigma；目录号71716)。要评价D-乳酸和L-乳酸的混合物，使用约50％D-乳酸和50％L-乳酸的混合物。将培养基的pH调整至pH 4.5，并在使用前无菌过滤。将接种的烧瓶在30℃下在75rpm轨道式震荡中孵育至少40小时。取样并使用利用折射率检测器的高效液相色谱法分析发酵期间发酵液中的葡萄糖、乙醇和乳酸浓度。
计算以下两个时间点之间的乳酸消耗率：接种时间(to)和发酵后期的另一个时间点(ti)。时间ti是接种后小时(h)的量度。在这两个时间点取样并测量两个样品中的乳酸浓度。在to处所取的样品中测得的乳酸浓度命名为[HLA]o并且单位为(gL-1)。同样地，在ti处所取的样品中测得的乳酸浓度命名为[HLA]i并且单位为(gL-1)。
然后如下计算乳酸消耗速率，得到单位(gL^h-1)：
乳酸消耗率＝([HLA]i-[HLA]o)/ti
当测量D-乳酸时，计算将产生D-乳酸消耗率。同样地，当测量L-乳酸时，计算将产生L-乳酸消耗速率；并且当测量总乳酸时，计算将产生总乳酸消耗速率。
例如，在接种时(to＝0h)和48小时后(ti＝48h)对摇瓶实验进行取样。测量两个样品中的总乳酸，结果如下：[HLA]o＝25.0gL-1；且[HLA]i＝15.9gL-1。然后如下计算总乳酸消耗率：
(25.0–15.9)/48＝0.190gL^h-1。
总乳酸消耗率为0.190gL^h-1。
实施例1：淀粉分解酿酒酵母菌株的产生
下文描述的是经遗传修饰的酿酒酵母菌株。所述菌株包括具有改善产乙醇酵母的乳酸消耗能力的经遗传修饰的菌株。
菌株1-3：ura3A酿酒酵母基础菌株
用SEQ ID NO:1转化菌株1(Ethanol RedTM)。SEQ ID NO:1含有以下元件：i)来自酿酒酵母(AR04-OFP)的突变形式的3-脱氧-D-阿拉伯-庚酮糖酸-7-磷酸(DAHP)合酶基因的表达盒；以及ii)用于靶向染色体整合到URA3基因座中的侧翼DNA。在含有3.5g/L对氟苯丙氨酸和lg/L L-酪氨酸的合成完全培养基(ScD-PFP)上选择转化体。将所得转化体在ScD-PFP上划线进行单菌落分离。选择单菌落。在单菌落中通过PCR验证将SEQ ID NO:1正确整合到基因座A的一个等位基因中。将PCR验证的分离株命名为菌株1-1。
用SEQ ID NO:2转化菌株1-1。SEQ ID NO:2含有以下元件：i)来自构巢曲霉(Aspergillus nidulans)的乙酰胺酶(amdS)基因的表达盒；以及ii)用于靶向染色体整合到URA3基因座中的侧翼DNA。在含有80mg/L尿嘧啶和lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上选择转化体。将所得转化体在含有80mg/L尿嘧啶和lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上划线进行单菌落分离。选择单菌落。在单菌落中通过PCR验证将SEQ ID NO:2正确整合到基因座A的第二等位基因中。将PCR验证的分离株命名为菌株1-2。
用SEQ ID NO:3和SEQ ID NO:4共转化菌株1-2。SEQ ID NO:3含有以下元件：i)来自PI噬菌体的ere重组酶的开放阅读框；以及ii)与SEQ ID NO:4同源的侧翼DNA。SEQ IDNO:4含有以下元件：i)2μ的复制起点；ii)来自酿酒酵母的URA3可选标记；以及iii)含有来自酿酒酵母的PGK启动子和CYC1终止子的酿酒酵母的侧翼DNA。在缺乏尿嘧啶的合成缺陷型培养基(ScD-Ura)上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落。筛选分离的菌落以在ScD-PFP以及含有80mg/L尿嘧啶和lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上生长。通过PCR验证缺失了AR04-OFP和amdS基因。将PCR验证的分离株划线至含有5-FOA的YNB，以针对2μ质粒缺失进行选择。将PCR验证的分离株命名为菌株1-3。
菌株1-4：在CYB2的一个等位基因处表达扣囊复膜酵母葡糖淀粉酶的两个密码子优化变体的酿酒酵母
用SEQ ID NO:5和SEQ ID NO:6共转化菌株1-3。SEQ ID NO:5含有以下元件：i)与天然CYB2基因的5'区同源的DNA；和ii)扣囊复膜酵母葡糖淀粉酶的独特密码子优化变体的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)URA3启动子以及URA3基因的一部分。SEQ ID NO:6含有以下元件：i)URA3基因的一部分和终止子；和ii)扣囊复膜酵母葡糖淀粉酶的独特密码子优化变体的表达盒，其处于PGK启动子和RPL3终止子的控制下；以及iii)与天然CYB2基因的3'区同源的DNA。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落。通过PCR验证在CYB2的一个等位基因处正确整合SEQID NO:5和SEQ ID NO:6。将PCR验证的分离株命名为菌株1-4。
菌株1-5：在CYB2的两个等位基因处表达扣囊复膜酵母葡糖淀粉酶的四个密码子优化变体的酿酒酵母
用SEQ ID NO:7和SEQ ID NO:8共转化菌株1-4。SEQ ID NO:7含有以下元件：i)与天然CYB2基因的5'区同源的DNA；和ii)扣囊复膜酵母葡糖淀粉酶的独特密码子优化变体的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)TEF1启动子和构巢曲霉乙酰胺酶基因(amdS)的一部分。SEQ ID NO:8含有以下元件：i)构巢曲霉乙酰胺酶基因(amdS)的一部分和ADH1终止子；和ii)扣囊复膜酵母葡糖淀粉酶的独特密码子优化变体的表达盒，其处于PGK启动子和RPL3终止子的控制下；以及iii)与天然CYB2基因的3'区同源的DNA。在含有80mg/L尿嘧啶和lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上选择转化体。将所得转化体在含有80mg/L尿嘧啶和lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上划线进行单菌落分离。。选择单菌落。通过PCR验证在CYB2的剩余等位基因处正确整合SEQ ID NO:7和SEQ ID NO:8。将PCR验证的分离株命名为菌株1-5。
菌株1-6：通过菌株1-5中的ere重组酶回收URA3和amdS标记
用SEQ ID NO:9转化菌株1-5。SEQ ID NO:9含有以下元件：i)来自酿酒酵母(AR04-OFP)的突变形式的3-脱氧-D-阿拉伯-庚酮糖酸-7-磷酸(DAHP)合酶基因的表达盒；2)来自PI噬菌体的ere重组酶的表达盒；3)含有天然URA3的表达盒；以及4)酿酒酵母CEN6着丝粒。在含有3.5g/L对氟苯丙氨酸和lg/L L-酪氨酸的合成完全培养基(ScD-PFP)上选择转化体。将所得转化体在ScD-PFP上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株1-6。
菌株1-8：用酿酒酵母IMA1的两个拷贝和麦卡特酵母(Saccharomyces mikatae)MALll-1的两个拷贝转化菌株1-6。
用SEQ ID NO:10转化菌株1-6。SEQ ID NO:10含有与整合基因座C的5'同源性、ScIMAl的表达盒、URA3标记的表达盒、SmMALll-1的表达盒以及与整合基因座C基因座的3’同源性。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落。通过PCR验证基因座C处整合的存在。将PCR验证的分离株保存为菌株1-7。
用SEQ ID NO:11转化菌株1-7。SEQ ID NO:11含有与整合基因座C的5'同源性、ScIMAl的表达盒、构巢曲霉乙酰胺酶基因(amdS)的表达盒、SmMALll-1的表达盒以及与整合基因座C的3'同源性。在含有lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上选择转化体。将所得转化体在含有lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上划线进行单菌落分离。选择单菌落。通过PCR验证在基因座C的剩余等位基因处正确整合SEQ ID NO:11。将PCR验证的分离株命名为菌株1-8。
菌株1-9：通过菌株1-8中的ere重组酶回收URA3和amdS标记
用SEQ ID NO:9转化菌株1-8。SEQ ID NO:9含有以下元件：i)来自酿酒酵母(AR04-OFP)的突变形式的3-脱氧-D-阿拉伯-庚酮糖酸-7-磷酸(DAHP)合酶基因的表达盒；2)来自PI噬菌体的ere重组酶的表达盒；3)含有天然URA3的表达盒；以及4)酿酒酵母CEN6着丝粒。在含有3.5g/L对氟苯丙氨酸和lg/L L-酪氨酸的合成完全培养基(ScD-PFP)上选择转化体。将所得转化体在ScD-PFP上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株1-9。
菌株1-10：在菌株1-9的原始基因座处恢复天然URA3
用SEQ ID NO:12转化菌株1-9。SEQ ID NO:12含有以下元件：1)天然URA3的表达盒、与菌株1-6中破坏的URA3基因座具有5'和3'同源性。在ScD-ura上选择转化体。将所得转化体在ScD-ura上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株1-10。
菌株1-11：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母细胞色素b2的酿酒酵母。
用SEQ ID NO:13(pGPL5)和SEQ ID NO:14(pGPL7)共转化菌株1-9。SEQ ID NO:13依序含有以下元件：1)对应于酿酒酵母CAN1基因的上游区的侧翼序列；2)含有TDH3启动子、SEQ ID NO:15中的酿酒酵母细胞色素b2(CYB2)和CYC1终止子的表达盒；以及3)loxP重组序列和乳清苷-5’-磷酸脱羧酶(URA3)的表达盒的5’部分。SEQ ID NO:14依序含有以下元件：1)乳清苷-5’-磷酸脱羧酶(URA3)的表达盒的3'部分和loxP重组序列；2)含有ADH1启动子和GAL10终止子的盒；以及4)对应于酿酒酵母CAN1基因的下游区的侧翼序列。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-11。
菌株1-12：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母细胞色素b2的酿酒酵母。
用SEQ ID NO:14和SEQ ID NO:16共转化菌株1-9。SEQ ID NO:16与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为东方伊萨酵母细胞色素b2(SEQ ID NO:17)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-12。
菌株1-13：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及黑曲霉乳酸氧化酶的酿酒酵母。
用SEQ ID NO:14和SEQ ID NO:18共转化菌株1-9。SEQ ID NO:18与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为去除了C-末端周围体靶向序列的黑曲霉乳酸氧化酶(SEQ ID NO:19)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-13。
菌株1-14：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及解脂耶氏酵母乳酸氧化酶的酿酒酵母。
用SEQ ID NO:14和SEQ ID NO:20共转化菌株1-9。SEQ ID NO:20与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为去除了C-末端周围体靶向序列的解脂耶氏酵母乳酸氧化酶(SEQ ID NO:21)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-14。
菌株1-15：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:22(pGPL6)和SEQ ID NO:23(pESL4)共转化菌株1-9。SEQ ID NO:22依序含有以下元件：1)对应于酿酒酵母CAN1基因的上游区的侧翼序列；2)含有TDH3启动子和CYCI终止子的盒；以及3)loxP重组序列和乳清苷-5’-磷酸脱羧酶(URA3)的表达盒的5’部分。SEQ ID NO:23依序含有以下元件：1)乳清苷-5’-磷酸脱羧酶(URA3)的表达盒的3'部分和loxP重组序列；2)含有ADH1启动子、在SEQ ID NO:24中发现的东方伊萨酵母单羧酸/质子同向转运蛋白(JENl)和GAL10终止子的表达盒；以及4)对应于酿酒酵母CAN1基因的下游区的侧翼序列。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-15。
菌株1-16：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:22和SEQ ID NO:25(pESLl)共转化菌株1-9。SEQ ID NO:25与SEQ IDNO:23类似，差异如下：SEQ ID NO:23中的东方伊萨酵母单羧酸/质子同向转运蛋白基因(JENl)被替换为酿酒酵母单羧酸/质子同向转运蛋白(JENl)基因(SEQ ID NO:26)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-16。
菌株1-17：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:22和SEQ ID NO:27(pESL7)共转化菌株1-9。SEQ ID NO:27与SEQ IDNO:23类似，差异如下：SEQ ID NO:23中的东方伊萨酵母单羧酸/质子同向转运蛋白基因(JENl)被替换为乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JENl)基因(SEQ ID NO:28)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-17。
菌株1-18：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母乙酸转运蛋白的酿酒酵母。
用SEQ ID NO:22和SEQ ID NO:29(pESL8)共转化菌株1-9。SEQ ID NO:29与SEQ IDNO:23类似，差异如下：SEQ ID NO:23中的东方伊萨酵母单羧酸/质子同向转运蛋白基因(JENl)被替换为酿酒酵母乙酸转运蛋白基因(SEQ ID NO:30)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-18。
菌株1-19：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及放线共生放线杆菌(Aggregatibacteractinomycetemcomitans)LDH和酿酒酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:25和SEQ ID NO:31共转化菌株1-9。SEQ ID NO:31与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为放线共生放线杆菌LDH(SEQ ID NO:32)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-19。
菌株1-20：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及鸭嘴兽(Ornithorhynchus anatinus)LDH和酿酒酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:25和SEQ ID NO:33共转化菌株1-9。SEQ ID NO:33与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为鸭嘴兽LDH(SEQ IDNO:34)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-20。
菌株1-21：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母细胞色素b2和东方伊萨酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:16和SEQ ID NO:23共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-21。
菌株1-22：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母细胞色素b2和酿酒酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:16和SEQ ID NO:25共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-22。
菌株1-23：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:16和SEQ ID NO:27共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-23。
菌株1-24：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母细胞色素b2和酿酒酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:13和SEQ ID NO:25共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-24。
菌株1-25：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:13和SEQ ID NO:27共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-25。
菌株1-26：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及黑曲霉乳酸氧化酶和酿酒酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:18和SEQ ID NO:25共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-26。
菌株1-27：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及解脂耶氏酵母乳酸氧化酶和酿酒酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:20和SEQ ID NO:25共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-27。
菌株1-28：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及醋酸杆菌(Acetobacter aceti)D-乳酸氧化酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:35和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:35与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为醋酸杆菌D-乳酸氧化酶(SEQ ID NO:36)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-28。
菌株1-29：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及氧化葡糖杆菌(Gluconobacter oxydans)D-乳酸氧化酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:37和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:37与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为氧化葡糖杆菌D-乳酸氧化酶(SEQ ID NO:38)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-29。
菌株1-30：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:39和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:39与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为酿酒酵母D-乳酸脱氢酶(SEQ ID NO:40)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-30。
菌株1-31：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母D-乳酸脱氢酶A和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:41和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:41与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为东方伊萨酵母D-乳酸脱氢酶(SEQ ID NO:42)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-31。
菌株1-32：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母D-乳酸脱氢酶B和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:43和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:43与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为东方伊萨酵母D-乳酸脱氢酶(SEQ ID NO:44)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-32。
菌株1-33：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及东方伊萨酵母D-乳酸脱氢酶C和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:45和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:45与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为东方伊萨酵母D-乳酸脱氢酶(SEQ ID NO:46)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-33。
菌株1-34：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；整合基因座控制区的酿酒酵母。
用SEQ ID NO:14和SEQ ID NO:22共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-34。
菌株1-35：表达乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:22和SEQ ID NO:27(pESL7)共转化菌株1-3。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-35。
菌株1-36：表达酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:13和SEQ ID NO:27共转化菌株1-3。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-36。
菌株1-37：表达酿酒酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:40和SEQ ID NO:27共转化菌株1-3。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-37。
菌株1-38：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及发酵乳杆菌(Lactobacillus fermentum)乳酸消旋酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:47和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:47与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为发酵乳杆菌乳酸消旋酶(SEQ ID NO:48)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-38。
菌株1-39：表达发酵乳杆菌乳酸消旋酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:47和SEQ ID NO:27共转化菌株1-3。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-39。
菌株1-40：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母细胞色素b2和酿酒酵母乙酸转运蛋白的酿酒酵母。
用SEQ ID NO:13和SEQ ID NO:29共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-25。
菌株1-41：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母细胞色素b2的两个拷贝和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:49和SEQ ID NO:50转化菌株1-25。SEQ ID NO:49依序含有以下元件：1)对应于酿酒酵母CAN1的上游区的侧翼序列；2)含有TDH3启动子、酿酒酵母CYB2和CYC1终止子的盒；以及3)loxP重组序列和构巢曲霉乙酰胺酶基因(amdS)的表达盒的5’部分。SEQID NO:50依序含有以下元件：1)构巢曲霉乙酰胺酶(amdS)的表达盒的3'部分和loxP重组序列；2)含有ADH1启动子、乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)和GAL10终止子的表达盒；以及4)对应于酿酒酵母CANl的下游区的侧翼序列。在含有lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上选择转化体。将所得转化体在含有lg/L乙酰胺作为唯一氮源的酵母氮源(无硫酸铵或氨基酸)上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-41。
菌株1-42：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及克鲁弗酵母(Saccharomyces kluyveri)细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:53和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:53与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为克鲁弗酵母细胞色素b2(SEQ ID NO:54)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-42。
菌株1-43：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及贝酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:55和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:55与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为贝酵母细胞色素b2(SEQID NO:56)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-43。
菌株1-44：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:57和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:57与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为鲁氏接合酵母细胞色素b2(SEQ ID NO:58)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-44。
菌株1-45：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及乳酸克鲁维酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:59和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:59与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为乳酸克鲁维酵母细胞色素b2(SEQ ID NO:60)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-45。
菌株1-46：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及多布克鲁维酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:61和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:61与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2基因被替换为多布克鲁维酵母细胞色素b2(SEQ ID NO:62)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-46。
菌株1-47：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及克鲁弗酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:63和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:63与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为克鲁弗酵母D-乳酸脱氢酶(SEQ ID NO:64)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-47。
菌株1-48：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及贝酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:65和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:63与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为贝酵母D-乳酸脱氢酶(SEQ ID NO:66)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-48。
菌株1-49：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及烟曲霉D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:67和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:67与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为烟曲霉D-乳酸脱氢酶(SEQ ID NO:68)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-49。
菌株1-50：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及乳酸克鲁维酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:69和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:69与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为乳酸克鲁维酵母D-乳酸脱氢酶(SEQ ID NO:70)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-50。
菌株1-51：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及多布克鲁维酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:71和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:71与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为多布克鲁维酵母D-乳酸脱氢酶(SEQ ID NO:72)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-51。
菌株1-52：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及马克斯克鲁维酵母D-乳酸脱氢酶和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:73和SEQ ID NO:27共转化菌株1-9。SEQ ID NO:73与SEQ ID NO:13类似，差异如下：SEQ ID NO:13中的酿酒酵母细胞色素b2被替换为马克斯克鲁维酵母D-乳酸脱氢酶(SEQ ID NO:74)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-52。
菌株1-53：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和多布克鲁维酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:75共转化菌株1-9。SEQ ID NO:80依序含有以下元件：1)对应于酿酒酵母CAN1的上游区的侧翼序列；2)含有PGK启动子、在SEQ ID NO:40中的酿酒酵母D-乳酸脱氢酶(DLDl)和RPL3终止子的表达盒；3)含有TDH3启动子、在SEQ ID NO:15中的酿酒酵母细胞色素b2(CYB2)和CYC1终止子的表达盒；以及4)loxP重组序列和乳清苷-5’-磷酸脱羧酶(URA3)的表达盒的5’部分。SEQ ID NO:75与SEQ ID NO:23类似，差异如下：SEQ ID NO:23中的东方伊萨酵母单羧酸/质子同向转运蛋白(JENl)被替换为多布克鲁维酵母单羧酸/质子同向转运蛋白(JENl)蛋白质(SEQ ID NO:76)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-53。
菌株1-54：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和马克思克鲁维酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:77共转化菌株1-9。SEQ ID NO:77与SEQ ID NO:23类似，差异如下：SEQ ID NO:23中的东方伊萨酵母单羧酸/质子同向转运蛋白(JEN1)被替换为马克思克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)(SEQ ID NO:78)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-54。
菌株1-55：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和解脂耶氏酵母单羧酸/质子同向转运蛋白(JEN6)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:79共转化菌株1-9。SEQ ID NO:79与SEQ ID NO:23类似，差异如下：SEQ ID NO:23中的东方伊萨酵母单羧酸/质子同向转运蛋白(JEN1)被替换为解脂耶氏酵母单羧酸/质子同向转运蛋白(JEN6)(SEQ ID NO:52)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-55。
菌株1-56：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:27共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-56。
菌株1-57：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶的两个拷贝、酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:81和SEQ ID NO:50共转化菌株1-56。SEQ ID NO:81与SEQ ID NO:80类似，差异在于URA3标记的5’部分被替换为amdS标记的5’部分。在YNB+乙酰胺平板上选择转化体。将所得转化体在YNB+乙酰胺平板上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-57。
菌株1-58：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和酿酒酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:25共转化菌株1-9。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-58。
菌株1-59：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和贝酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:82共转化菌株1-9。SEQ ID NO:82与SEQ ID NO:50类似，差异如下：SEQ ID NO:50中的乳酸克鲁维酵母JENl被替换为贝酵母JENl(SEQ ID NO:83)。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-59。
菌株1-60：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JENl)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:84共转化菌株1-9。SEQ ID NO:84与SEQ ID NO:27类似，差异在于ADH1启动子SEQ ID NO:27被替换为GPD1启动子。在ScD-Ura上选择转化体，并将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。
将通过PCR确认含有表达盒的单拷贝的三种独立的转化体用SEQ ID NO:81和SEQID NO:87转化，以获得表达盒的第二拷贝。SEQ ID NO:87与SEQ ID NO:50类似，差异在于SEQ ID NO:50中的ADH1启动子被替换为GPD1启动子。在YNB+乙酰胺平板上选择转化体。将所得转化体在YNB+乙酰胺平板上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-60。
菌株1-61：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:85共转化菌株1-9。SEQ ID NO:85与SEQ ID NO:27类似，差异在于ADH1启动子SEQ ID NO:27被替换为ADH2启动子。在ScD-Ura上选择转化体，并将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。
将通过PCR确认含有表达盒的单拷贝的三种独立的转化体用SEQ ID NO:81和SEQID NO:88转化，以获得表达盒的第二拷贝。SEQ ID NO:88与SEQ ID NO:50类似，差异在于SEQ ID NO:50中的ADH1启动子被替换为ADH2启动子。在YNB+乙酰胺平板上选择转化体。将所得转化体在YNB+乙酰胺平板上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-61。
菌株1-62：表达修饰的扣囊复膜酵母葡糖淀粉酶；酿酒酵母异麦芽糖酶和麦卡特酵母麦芽糖转运蛋白；以及酿酒酵母D-乳酸脱氢酶、酿酒酵母细胞色素b2和乳酸克鲁维酵母单羧酸/质子同向转运蛋白(JEN1)的酿酒酵母。
用SEQ ID NO:80和SEQ ID NO:86共转化菌株1-9。SEQ ID NO:86与SEQ ID NO:27类似，差异在于ADH1启动子SEQ ID NO:27被替换为PDC1启动子。在ScD-Ura上选择转化体，并将所得转化体在ScD-Ura上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。
将通过PCR确认含有表达盒的单拷贝的三种独立的转化体用SEQ ID NO:81和SEQID NO:89转化，以获得表达盒的第二拷贝。SEQ ID NO:89与SEQ ID NO:50类似，差异在于SEQ ID NO:50中的ADH1启动子被替换为PDC1启动子。在YNB+乙酰胺平板上选择转化体。将所得转化体在YNB+乙酰胺平板上划线进行单菌落分离。选择单菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试三种独立的转化体，并将代表性的分离株命名为菌株1-62。
表1工程酵母的总结描述
实施例2：使用乙醇发酵摇瓶测定评估工程化酿酒酵母中的L-乳酸消耗
下文描述的是使用来自实施例1的选定酵母菌株进行的乙醇发酵试验的评估结果和相关数据。菌株1和菌株1-10是用作各种工程化菌株的比较物的参考菌株。菌株1是具有商业上合适的乙醇耐受性的酿酒酵母。菌株1-10是来源于菌株1的菌株，其也适用于商业乙醇生产。
使用限定培养基进行摇瓶评估。
将来自表1中列出的菌株的选定菌株在ScD-Ura平板上划线，然后在30℃下孵育直至可见单菌落(1-2天)或者在30℃/250rpm下在14ml培养管中作为液体培养物的4ml YP+100g/L D-葡萄糖中生长过夜以产生种子生物质。用种子细胞浆液接种摇瓶以达到0.1-0.3的初始OD600。在即将接种之前，将60mL摇瓶培养基添加到250mL的无挡板摇瓶(Corning4995-250)中，该摇瓶装有含透气密封的螺旋盖(corning 1395-45LTMC)。摇瓶培养基根据Verduyn(Verduyn等人,1992,Yeast.第8,501-517卷)制备，不同之处在于用二水合锰代替四水合锰。向培养基补充D-葡萄糖，以使初始D-葡萄糖浓度达到250克至300克D-葡萄糖/升培养基之间。另外，向培养基补充L-乳酸钠(Sigma；目录号L7022)。将培养基的pH调整至pH4.5，并在使用前无菌过滤。
将接种的烧瓶在30℃下在75rpm轨道式震荡中孵育至少40小时。取样并使用利用折射率检测器的高效液相色谱法分析发酵期间发酵液中的葡萄糖、乙醇和乳酸浓度。
实施例2A：评估菌株1-9中CYB2的过表达
如下表2中所示，具有天然CYB2敲除的菌株(菌株1-10)似乎不消耗任何L-乳酸。L-乳酸增加可能是由于在发酵期间发生水蒸发，导致浓度轻微增加。然而，含有过表达的CYB2的菌株(菌株1-11和菌株1-12)与具有天然CYB2的菌株(菌株1)的表现相似。具体地，具有CYB2的菌株消耗非常少量的L-乳酸，同时主要通过消耗葡萄糖产生商业上显著的乙醇量。
表2.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的CYB2的菌株)的L-乳酸消耗。
实施例2B：评估菌株1-9中CYB2和酿酒酵母JEN1的过表达
如下表3中所示，具有过表达的CYB2和过表达的酿酒酵母JEN1的菌株(菌株1-22和菌株1-24)显示出显著的L-乳酸消耗，且发酵液中仍存在大量葡萄糖。然而，具有天然CYB2和JEN1的菌株(菌株1)基本上仅在葡萄糖浓度非常低之后才消耗L-乳酸，而没有CYB2的菌株(菌株1-10、菌株1-16和菌株1-34)不消耗L-乳酸。图1A-图1C示出对应于第二发酵摇瓶试验的菌株1-24的数据。
表3.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的CYB2和酿酒酵母JEN1的菌株)的L-乳酸消耗。
实施例2C：评估菌株1-9中CYB2的过表达和Kl JENl和ScADY2的包含
如下表4中所示，具有天然CYB2、JENl和ADY2的菌株在葡萄糖存在下显示出最小限度的L-乳酸消耗。具有过表达的CYB2及过表达的JENl(菌株1-24)抑或突变的ADY2(菌株1-40)的菌株在葡萄糖存在下显示出显著更高的L-乳酸消耗。具有过表达的CYB2和乳酸克鲁维酵母JENl的菌株(菌株1-25)具有最高的L-乳酸消耗。此外，菌株1-24、菌株1-25和菌株1-40显示出比菌株1显著更高的乙醇滴度。
表4.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的CYB2及乳酸克鲁维酵母JENl抑或突变ScADY2的菌株)的L-乳酸消耗。
实施例2D：评估菌株1-9中乳酸氧化酶的过表达
具有过表达的乳酸氧化酶(L-LOX)的菌株预期可显示出改善的L-乳酸消耗。然而，如下表5中所示，具有过表达的L-LOX的菌株(菌株1-13和菌株1-14)与比较菌株相比并未显示出改善的L-乳酸消耗。
表5.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的乳酸氧化酶的菌株)的L-乳酸消耗。
实施例2E：评估菌株1-9中乳酸氧化酶和过表达的JENl的过表达
如上文所讨论，具有过表达的乳酸氧化酶(L-LOX)的菌株预期可显示出改善的L-乳酸消耗，但并未显示出改善的L-乳酸消耗。此外，如下表6中所示，具有过表达的JENl和L-LOX的菌株(菌株1-26和菌株1-27)也未显示出改善的L-乳酸消耗。
另外，具有东方伊萨酵母CYB2和东方伊萨酵母JENl的菌株(菌株1-21)表现出与菌株1相同的L-乳酸消耗，但乙醇产生和葡萄糖消耗较低，即使亲本野生型生物体(东方伊萨酵母)可在L-乳酸作为碳源的情况下生长。
表6.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的乳酸氧化酶和过表达的JENl的菌株，以及具有东方伊萨酵母CYB2和东方伊萨酵母JENl的菌株)的L-乳酸消耗。
实施例2F：评估菌株1-9中乳酸脱氢酶与Sc JEN1组合的过表达
包含过表达的乳酸脱氢酶的菌株预期可显示出改善的L-乳酸消耗，特别是如果预期厌氧发酵的话。然而，表7显示包含乳酸脱氢酶(LDH)的菌株(菌株1-19和菌株1-20)实际上产生L-乳酸而不是消耗L-乳酸。
表7.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的乳酸脱氢酶和JEN1转运蛋白的菌株)的L-乳酸消耗。
实施例2G：评估菌株h9中的各种异源CYB2基因与乳酸克鲁维酵母JEN1
如表8中所示，含有鲁氏接合酵母CYB2的菌株(菌株1-44)或含有乳酸克鲁维酵母CYB2的酵母(菌株1-45)显示出比具有Sc CYB2的菌株更高的L-乳酸消耗和更高的乙醇滴度。具有其他CYB2的菌株(1-42、1-43和1-46)显示出显著的L-乳酸消耗和商业上相关的乙醇滴度。
表8.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的CYB2和乳酸克鲁维酵母JENl的菌株)的L-乳酸消耗
实施例21：评估菌株1-9中的各种异源单羧酸/质子同向转运蛋白
如表9中所示，具有克鲁维酵母JENl的两种菌株(多布克鲁维酵母，菌株1-53；乳酸克鲁维酵母，菌株1-56)显示出最高的乳酸消耗和乙醇滴度。包含马克斯克鲁维酵母JENl的菌株(菌株1-54)和包含解脂耶氏酵母JEN6的菌株(菌株1-55)与参考菌株相比，也显示出改善的乳酸消耗。
表9.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达酿酒酵母DLD1、酿酒酵母CYB2和JEN1或JEN6的菌株)的总乳酸(L-乳酸和D-乳酸的混合物)消耗。
实施例3：使用乙醇发酵摇瓶测定评估工程化酿酒酵母中的D-乳酸消耗
下文描述的是使用来自实施例1的选定酵母菌株进行的乙醇发酵试验的评估结果和相关数据。如实施例2中所述进行摇瓶评估，不同之处在于使用D-乳酸钠(Sigma；目录号71716)代替L-乳酸钠。
实施例3A：评估菌株1-9中JEN1转运蛋白的过表达
如表10和图2A-图2C中所示，含有过表达的乳酸克鲁维酵母JEN1的菌株与参考菌株(菌株1和菌株1-10)或含有其他过表达的JENl基因的菌株(菌株1-15和1-16)相比，具有显著更高的D-乳酸消耗以及显著更高的乙醇滴度。
表10.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的JENl的菌株)的D-乳酸消耗。
实施例3B：评估菌株1-9中酿酒酵母ADY2(ADY2Leu2i9Vai，Ala252Gly>通透酶的过 表达
如表11中所示，含有乳酸克鲁维酵母JENl的菌株中的D-乳酸消耗和乙醇产生显著高于参考菌株，而含有ADY2的菌株(菌株1-18)显示出与参考菌株相似的性能。
表11.发酵摇瓶测定中含有过表达的JENl的酿酒酵母菌株的D-乳酸消耗。
实施例3C：评估菌株1-9中D-乳酸脱氢酶和乳酸克鲁维酵母JEN1的过表达
如图3A-图3C中所示，含有乳酸克鲁维酵母JENl和过表达的DLD的菌株(菌株1-30)显示出相比参考菌株显著改善的D-乳酸消耗和更高的乙醇滴度。此外，菌株1-30的D-乳酸消耗相比仅具有乳酸克鲁维酵母JENl的菌株(菌株1-17)显著改善。
实施例3D：评估菌株1-9中的东方伊萨酵母D-乳酸脱氢酶和乳酸克鲁维酵母JENl
下表12示出含有乳酸克鲁维酵母JENl和过表达的东方伊萨酵母DLD的三种同源菌株(菌株1-31、菌株1-32和菌株1-33)的数据。与参考菌株(1-10)相比，这三种同源物各自都显示出改善的D-乳酸消耗。
表12.发酵摇瓶测定中含有过表达的D-乳酸氧化酶和乳酸克鲁维酵母JENl的酿酒酵母菌株的D-乳酸消耗。
实施例3E：评估菌株1-9中的过表达D-乳酸氧化酶和乳酸克鲁维酵母JENl
如下表13中所示，含有过表达的D-乳酸氧化酶(D-LOX)和乳酸克鲁维酵母JENl的菌株(菌株1-28和菌株1-29)与参考菌株(菌株1和菌株1-10)相比显示出改善的D-乳酸消耗，但与仅含有乳酸克鲁维酵母JENl的菌株(菌株1-17)相比显示出更少的D-乳酸消耗。含有D-LOX的菌株预期可显示出改善的D-乳酸消耗，然而，如上述L-LOX菌株所示，包含D-LOX并未改善D-乳酸消耗。
表13.发酵摇瓶测定中含有过表达的D-乳酸氧化酶和乳酸克鲁维酵母JENl的酿酒酵母菌株的D-乳酸消耗。
实施例3F：评估菌株1-9中的各种异源DLD基因与乳酸克鲁维酵母JENl
如表14中所示，与具有选定DLD的其他菌株相比，含有乳酸克鲁维酵母DLD的菌株(1-50)具有最高的乙醇滴度和L-乳酸消耗。然而，其他菌株(1-47、1-48、1-49、1-51和1-52)均显示出显著的D-乳酸消耗和商业上相关的乙醇滴度。
表14.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的DLD1和乳酸克鲁维酵母JENl的菌株)的D-乳酸消耗。
实施例4：在同时糖化发酵(SSF)摇瓶测定中评估经遗传修饰的酿酒酵母菌株
评估含有过表达的酿酒酵母CYB2和乳酸克鲁维酵母JEN1的酿酒酵母菌株的乳酸和D-葡萄糖的消耗，以及乙醇产生。如实施例1中所述获得菌株。
将菌株敲入ScD-ura平板并在25℃下孵育直至可见单菌落(2-3天)。将来自ScD-ura平板的细胞刮入无菌摇瓶培养基中，并且测量光密度(ODβoo)。使用Genesys20型分光光度计(Thermo Scientific)在600nm波长和1cm路径长度下测量光密度。用细胞浆液接种摇瓶以达到0.1的初始ODδoo。在即将接种之前，将50mL摇瓶培养基添加到250mL的无挡板摇瓶(Corning 4995-250)中，该摇瓶装有含透气密封的螺旋盖(corning 1395-45LTMC)。摇瓶培养基由850g部分水解的玉米淀粉、150g过滤过的轻质浸泡水、lOg水、25g葡萄糖和lg尿素组成。将每种菌株的一式两份烧瓶在30℃下在100rpm轨道式震荡中孵育至少48小时。取样并通过利用折射率检测的HPLC和利用ELSD检测的UPLC分析发酵期间发酵液中的代谢物浓度。乳酸(D-乳酸和L-乳酸两者)是轻质浸泡水的组分并且是通过产乳酸细菌污染轻质浸泡水而产生的。轻质浸泡水中D-乳酸和L-乳酸的分数在各批次之间变化，并且绝对量是不可预测的。通常，D-乳酸和L-乳酸的分数是两种对映体的50:50外消旋混合物，其总浓度为每升轻质浸泡水大约25克乳酸。轻质浸泡水还含有低水平的葡萄糖。
如图4A和图4B中所示，含有过表达的CYB2和乳酸克鲁维酵母JEN1的菌株(菌株1-25)与参考菌株相比，消耗大部分或全部D,L-乳酸，同时产生相似量的乙醇。
实施例5：利用相对低水平的葡萄糖和相对高水平的DX-乳酸的SSF中经遗传修饰 的酵母菌株的性能
如实施例1中所述获得菌株1-41。菌株1-41是含有ScCYB2的2个拷贝和K1JEN1的2个拷贝的菌株1-9。根据实施例4在摇瓶发酵中测试菌株，差异如下：1)本实施例(实施例5)的摇瓶培养基是轻质浸泡水；2)用细胞浆液接种摇瓶以达到4.0的初始OD6oo；3)无挡板摇瓶装有Morton盖；并且4)将摇瓶在轨道振荡器中以150rpm孵育。
如表15和表16以及图5A和图5B中所示，含有过表达的ScCYB2并且还含有K1JEN1的菌株(1-41)与参考菌株相比消耗显著更多的D,L-乳酸，同时产生显著更高的乙醇滴度。
表15：同时糖化摇瓶测定中菌株1-9中ScCYB2和Kl JEN1的过表达
表16：总乳酸(D-乳酸和L-乳酸)消耗速率
实施例6：具有选定异源一元羧酸/单羧酸转运蛋白的经遗传修饰的酵母菌株中的L-乳酸消耗
评估含有选定异源一元羧酸/单羧酸转运蛋白的酿酒酵母菌株的L-乳酸转运/消耗。如先前所示，与L-乳酸转运相比，乳酸克鲁维酵母JEN1对D-乳酸转运具有更高的亲和力。使用仅存在L-乳酸的限定培养基进行摇瓶测定。未测量右旋糖浓度，但预期在20h时为约150g/L。
如表17和图6中所示，与参考菌株(菌株1和菌株1-10)相比，所测试的所有异源一元羧酸/单羧酸转运蛋白都表现出改善的L-乳酸消耗。
表17.发酵摇瓶测定中酿酒酵母菌株(包括含有过表达的酿酒酵母DLDl、酿酒酵母CYB2、选定异源一元羧酸/单羧酸转运蛋白的菌株)的L-乳酸消耗。
实施例7：生产规模的乙醇发酵工艺中经遗传修饰的酵母菌株的性能
使用无乳酸消耗途径的参考菌株(菌株1-10)并(单独地)使用具有激活的外源乳酸消耗途径的菌株(菌株1-57)，利用包含轻质浸泡水(LSW)的初始发酵培养基以商业规模进行用于生产乙醇的连续发酵工艺。第1阶段是繁殖器，第2阶段是预发酵器。
不同阶段的D/L-乳酸浓度的变化反映了由于入口物流和发酵液稀释而导致的发酵液组成的许多变化。每个阶段的每种菌株的稀释水平基本上相同。因此，相关的比较是在任何给定阶段内菌株1-10与菌株1-57的D/L-乳酸的变化比率。
如表18中所示，相对于具有激活的外源乳酸消耗途径的菌株(菌株1-57)，具有天然CYB2敲除且没有外源乳酸消耗途径的菌株(菌株1-10)在第1阶段和第2阶段各个阶段中均显示出更高的L-乳酸和D-乳酸浓度。
表18：在生产规模上经遗传修饰的酵母菌株的乳酸消耗
实施例8：评估用于驱动JEN1转运蛋白表达的启动子
评估菌株1-57中所用启动子(ADH1)的替代启动子对JEN1转运蛋白表达的驱动。在同时糖化发酵(SSF)摇瓶测定中评估菌株。这些摇瓶用作评定选定菌株的早期发酵负担的代表。
在一个方面，如表19中所示，具有菌株1-57中所用启动子的替代启动子的菌株(即菌株1-60、菌株1-61和菌株1-62)在中点(22h)显示出显著更高的乙醇滴度，因此表现出减少的发酵负担。
在另一方面，与所有其他菌株相比，菌株1-57显示产生显著更少的甘油，乙醇滴度相应显著增加。具有激活的外源乳酸消耗途径的所有菌株均显示出乳酸消耗，且产生与参考菌株相比相同或更高的最终乙醇滴度。这表明具有激活的外源乳酸消耗途径的菌株与选定启动子组合可在较早的时间点获得更高的乙醇滴度和/或相同的乙醇滴度，这两者都可提供显著的商业优势(即，增加的生产量或减少的周期时间，或两者兼而有之)。
表19：选定菌株的SSF摇瓶测定
实施例9：利用选定菌株的分批SSF
在实验室规模的分批发酵工艺中针对菌株1-10对菌株1-62进行基准测试。原料由部分糖化的淀粉物流组成，其起始右旋糖浓度为20％-35％。起始pH在3.5与4.5之间，并且操作温度为31℃。从20小时经过发酵时间(EFT)至发酵结束(40小时EFT)记录的平均OUR为0.66mmol 0 2/(L-h)，并运行容器直至游离右旋糖浓度为0.2-0.5(40小时EFT)。这些数据代表每种菌株的三个独立发酵容器的平均值。
使用碳水化合物柱(87C)交叉验证起始右旋糖浓度，以确定T0+样品中的游离右旋糖、DP2、DP3和DP4+浓度，将这些浓度乘以它们各自的化学增益因数以确定右旋糖总和。使用酶水解方法交叉验证该方法。对每个容器的右旋糖总和结果取平均值。
如表20中所示，与参考菌株相比，菌株1-62(具有外源乳酸消耗途径)表现出显著更高的乙醇滴度和乳酸消耗，并且还显示出显著更低的甘油生成。
表20：菌株1-10与菌株1-62的4L SSF分批发酵器数据
示例性实施方案
此部分中的实施方案依次用字母A到Z，然后是AA到AZ，然后BA以此类推来标明。
A.一种经遗传修饰的酵母，其包含编码一元羧酸/单羧酸转运蛋白的异源基因和一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(分类为EC 1.1.2.3或1.1.2.4)，其中当所述酵母存在于包含乳酸和己糖的发酵培养基中时，所述酵母能够消耗乳酸并产生乙醇。
B.如实施方案A所述的酵母，其中当在21h取样时间所述发酵培养基中己糖的浓度为150g/L或更高时，如根据乳酸消耗评估方案所测量的，所述酵母具有至少0.030 0.035、0.040、0.045或0.050gL^h-1的L-乳酸消耗速率。
C.如实施方案A所述的酵母，其中当在48h取样时间所述发酵培养基中己糖的浓度为5g/L或更高时，如根据乳酸消耗评估方案所测量的，所述酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL-1h-1的L-乳酸消耗速率。
D.如实施方案A所述的酵母，其中当在21h取样时间所述发酵培养基中己糖的浓度为150g/L或更高时，如根据乳酸消耗评估方案所测量的，所述酵母具有至少0.005、0.006、0.007、0.008、0.009、0.010、0.015、0.020、0.025或0.030gL^h-1的L-乳酸消耗速率。
E.如实施方案A所述的酵母，其中当在48h取样时间所述发酵培养基中己糖的浓度为5g/L或更高时，如根据乳酸消耗评估方案所测量的，所述酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的D-乳酸消耗速率。
F.如实施方案A-E中任一项所述的酵母，其中所述酵母的D-乳酸、L-乳酸和/或总乳酸消耗速率大于不含编码一元羧酸/单羧酸转运蛋白的异源基因的酵母。
G.如实施方案A所述的酵母，其中在同时糖化发酵(SSF)工艺中的0与7小时时间之间，所述酵母具有至少0.550、0.555、0.600或0.650gL^h-1的总乳酸消耗速率。
H.如实施方案A所述的酵母，其中在SSF工艺中的7小时与48小时时间之间，所述酵母具有至少0.130、0.140、0.150、0.160或0.170gL^h-1的总乳酸消耗速率。
I.如实施方案A所述的酵母，其中在SSF工艺中的0与48小时时间之间，所述酵母具有至少0.200、0.210、0.220、0.230、0.240或0.250gL^h-1的总乳酸消耗速率。
J.如实施方案G-I中任一项所述的酵母，其中所述总乳酸消耗速率根据实施例5中的SSF摇瓶测定的方法来确定。
K.如实施方案A-J中任一项所述的酵母，其中所述酵母能够消耗D-乳酸、L-乳酸或它们的混合物。
L.如实施方案A-K中任一项所述的酵母，其中所述一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的D-乳酸脱氢酶(DLD)基因。
M.如实施方案A-K中任一项所述的酵母，其中所述一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的细胞色素b2(CYB2)基因。
N.如实施方案A-K中任一项所述的酵母，其中所述一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的DLD基因和过表达的CYB2基因。
O.如实施方案L-N中任一项所述的酵母，其中所述一种或多种DLD基因来自以下酵母物种中的一种或多种：酿酒酵母、东方伊萨酵母、克鲁弗酵母、贝酵母、多布克鲁维酵母、马克斯克鲁维酵母或乳酸克鲁维酵母。
P.如实施方案A-O中任一项所述的酵母，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:64、SEQID NO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ ID NO:72或SEQ ID NO:74。
Q.如实施方案M-N中任一项所述的酵母，其中所述一种或多种CYB2基因来自以下酵母物种中的一种或多种：酿酒酵母、东方伊萨酵母、克鲁弗酵母、贝酵母、鲁氏接合酵母、多布克鲁维酵母或乳酸克鲁维酵母。
R.如实施方案A-Q中任一项所述的酵母，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:15、SEQ ID NO:17、SEQ ID NO:54、SEQID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62。
S.如实施方案A-R中任一项所述的酵母，其包含编码乳酸消旋酶的异源基因。
T.一种经遗传修饰的酵母，其包含编码单羧酸/质子同向转运蛋白的异源基因和编码葡糖淀粉酶的异源基因，其中当所述酵母存在于包含乳酸和己糖、淀粉或己糖与淀粉的组合的发酵培养基中时，所述酵母能够消耗乳酸并产生乙醇。
U.如实施方案A-T中任一项所述的酵母，其中所述酵母编码来自扣囊复膜酵母或米根霉的葡糖淀粉酶。
V.如实施方案A-U中任一项所述的酵母，其中所述酵母属于酵母属。
W.如实施方案V所述的酵母，其中所述酵母属于酿酒酵母物种。
X.一种经遗传修饰的酵母，其包含酿酒酵母物种的酵母，其中所述酵母包含编码单羧酸/质子同向转运蛋白的异源基因。
Y.如实施方案X所述的酵母，其中所述酵母通过经遗传修饰的酿酒酵母宿主酵母获得，并且其中所述宿主酵母是ETHANOL REDTM(可从Lesaffre商购获得)。
Z.如实施方案A-Y中任一项所述的酵母，其能够以至少1.0g L-1h-1、2.0g L-1h-1、3.0g L-1h-1、3.3g L-1h-1或3.75g L-1h-1的发酵生产速率产生乙醇。
AA.如实施方案A-Z中任一项所述的酵母，其能够以大于80g/L、90g/L、100g/L、110g/L、120g/L、125g/L 130g/L、135g/L、140g/L、145g/L、150g/L、155g/L、160g/L或165g/L的滴度产生乙醇。
AB.如实施方案A-AA中任一项(即，A-Z和AA)所述的酵母，其(a)能够以大于90g/L、100g/L、110g/L、120g/L、130g/L或140g/L的滴度产生乙醇；(b)在33℃至40℃、33℃至39℃、33℃至38℃、33℃至37℃、34℃至37℃、35℃至37℃或36℃至38℃范围内的温度下耐热；或(a)和(b)两者兼而有之。
AC.如实施方案A-AB中任一项所述的酵母，其中所述编码一元羧酸/单羧酸转运蛋白的异源基因来自克鲁维酵母属的酵母。
AD.如实施方案A-AC中任一项所述的酵母，其中所述编码一元羧酸/单羧酸转运蛋白的异源基因来自乳酸克鲁维酵母。
AE.如实施方案A-AD中任一项所述的酵母，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQ ID NO:76或SEQ ID NO:78。
AF.如实施方案AE所述的酵母，其包含在SEQ ID NO:15、SEQ ID NO:17、SEQ IDNO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62中的指定位置处的以下残基中的一者或多者：Lys349、Tyrl43、Tyr254和His373。
AG.如实施方案A-AF中任一项所述的酵母，其用于生产乙醇或除乙醇之外的生物产物。
AH.一种生产乙醇的工艺，所述工艺包括用如实施方案A-AG中任一项所述的酵母使底物发酵。
AI.一种用于制造乙醇的连续发酵工艺，所述工艺包括：提供包含己糖或己糖低聚物的发酵培养基；用包含异源单羧酸/质子同向转运蛋白(JEN1)基因的经遗传修饰的酵母使所述发酵培养基发酵；向所述发酵培养基中添加一种或多种包含乳酸的进料物流；以及从所述发酵培养基中移出至少一种包含乙醇的输出物流，其中所述发酵培养基的平均己糖或己糖低聚物浓度为至少5g/L，所述工艺的体积氧摄取速率为至少0.5mmol(V(L·h)，并且所述工艺以至少1.0g L-1h-1的总速率生产乙醇且所述酵母消耗乳酸，其中所述至少一种输出物流含有小于90％的在所述一种或多种进料物流中添加的乳酸。
AJ.如实施方案AI所述的工艺，其中所述一种或多种进料物流的乳酸浓度具有至少1g/L的平均乳酸浓度。
AK.如实施方案AI-AJ中任一项所述的工艺，其中所述进料物流是植物工艺物流。
AL.如实施方案AK所述的工艺，其中所述植物工艺物流是玉米工艺物流或小麦工艺物流。
AM.如实施方案AH-AL中任一项所述的工艺，其中在预发酵器步骤结束时的乙醇滴度在20至80g/L的范围内。
AN.如实施方案AH-AM中任一项所述的工艺，其中繁殖器步骤或预发酵器步骤中的L-乳酸消耗速率为至少0.030 0.035、0.040、0.045或0.050g L-1h-1。
AO.如实施方案AH-AN中任一项所述的工艺，其中繁殖器步骤或预发酵器步骤中的D-乳酸消耗速率为至少0.005、0.006、0.007、0.008、0.009、0.010、0.015、0.020、0.025或0.030gL^h-1。
AP.如实施方案AH-AO中任一项所述的工艺，其中所述至少一种输出物流的乙醇滴度为至少90、100、110、120、130、140、150或160g/L。
AQ.一种用于制造乙醇的工艺，所述工艺包括：使用包含异源单羧酸/质子同向转运蛋白基因的经遗传修饰的酵母使培养基发酵，其中在发酵开始时所述培养基包含浓度为至少5g/L的葡萄糖或葡萄糖低聚物，在发酵开始时所述培养基的乳酸含量为至少1g/L，在发酵结束时至少40％的乳酸被消耗，并且最终乙醇滴度为至少90g/L。
AR.如实施方案AH-AQ中任一项所述的工艺，其具有至少0.5、1、2、3或4mmol 02/(L-h)的体积氧摄取速率(OUR)。
AS.一种用于制造乙醇的工艺，所述工艺包括：使用酿酒酵母使培养基发酵，其中所述培养基包含葡萄糖或葡萄糖低聚物和乳酸，OUR为至少0.5mmol 02/(L-h)，在发酵开始时所述培养基的乳酸含量为至少1g/L，至少35％的在发酵开始时存在于所述培养基中和/或添加到所述培养基中的乳酸在发酵结束时被消耗，并且最终乙醇滴度为至少90g/L。
AT.如实施方案AS所述的工艺，其中所述OUR为至少1、2、3、4、5、6、7、8、9或10mmol02/(L-h)。
AU.如实施方案AS-AT中任一项所述的工艺，其中在发酵开始时所述培养基的乳酸含量为至少2、3、4或5g/L。
AV.如实施方案AS-AU中任一项所述的工艺，其中至少40％、45％或50％的在发酵开始时存在于所述发酵培养基中和/或添加到所述发酵培养基中的乳酸在发酵结束时被消耗
AW.如实施方案AH-AV中任一项所述的工艺，其中所述酵母是如实施方案A-Y中任一项所述的酵母。
AX.如实施方案AH-AW中任一项所述的工艺，其中所述乳酸是L-乳酸、D-乳酸或它们的混合物。
AY.如实施方案AH-AX中任一项所述的工艺，其中所述发酵培养基的pH在约2至7的范围内。
AZ.如实施方案AH-AY中任一项所述的工艺，其中发酵温度在25℃至45℃、25℃至40℃、25℃至35℃、30℃至40℃或28℃至38℃的范围内。
BA.如实施方案AH-AZ中任一项所述的工艺，其中所述发酵培养基的干固体为至少30、40、50、60、70或80g/L
BB.如实施方案AH-BA中任一项所述的工艺，其中发酵进料的干固体为120-150g/L。
BC.如实施方案AH-BB中任一项所述的工艺，其中发酵结束时的乙醇滴度为至少80、90、100、110、120、130、135、140、145、150、155或160g/L。
BD.一种用于产生具有改善的乳酸消耗的经遗传修饰的酵母的方法，所述方法包括过表达一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(分类为EC 1.1.2.3或1.1.2.4)或异源单羧酸/质子同向转运蛋白，并使酵母经历进化以获得特征，其中当所述酵母存在于包含乳酸和葡萄糖的发酵培养基中时，所述酵母能够消耗乳酸并产生乙醇。
BE.如实施方案BD所述的方法，其中所述特征是乳酸的生长速率增加。
BF.如实施方案AH-BE中任一项所述的工艺或方法，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:24、SEQ ID NO:26、SEQ IDNO:28、SEQ ID NO:76或SEQ ID NO:78。
BG.如实施方案BF所述的工艺或方法，其中所述酵母包含在SEQ ID NO:15、SEQ IDNO:17、SEQ ID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62中的指定位置处的以下残基中的一者或多者：Lys349、Tyrl43、Tyr254和His373。
BH.如实施方案A-BG中任一项所述的工艺、方法或酵母，其中所述酵母包含异源核酸调控序列。
BI.如实施方案BH所述的工艺、方法或酵母，其中所述异源核酸调控序列与编码一元羧酸/单羧酸转运蛋白的异源基因相关。
BJ.如实施方案BH或BI所述的工艺、方法或酵母，其中所述异源核酸调控序列包含ADH1启动子。
BK.如实施方案BH或BI所述的工艺、方法或酵母，其中所述异源核酸调控序列包含ADH2启动子、PDCl启动子或GPD1启动子。
还应理解，上述工艺、方法或组合物的上述任何实施方案的要素或方面可应用于任何其他实施方案，即使本文未明确说明。
本文提到的每个专利、专利申请或公布的公开内容以引用方式整体并入本文。虽然已参照具体的实施方案公开了本发明，但本发明的其他实施方案和变型形式可由本领域的技术人员在不脱离本发明的实质和范围的情况下设计。所附权利要求旨在被视为包括所有此类实施方案和变型形式。
序列表
<110> 嘉吉公司
格雷戈里·波因特
布莱恩·拉什
阿琳·福斯默
克里斯托弗·米勒
乔纳森·维尔德豪斯
<120> 经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺
<130> N00478USP1
<150> 62/395,792
<151> 2016-09-16
<160> 89
<170> PatentIn 3.5版
<210> 1
<211> 3182
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 1
cctactgcgc caattgatga caatacagac gatgataaca aaccgaagtt atctgatgta 60
gaaaaggatt aaagatgcta agagatagtg atgatatttc ataaataatg taattctata 120
tatgttaatt accttttttg cgaggcatat ttatggtgaa ggataagttt tgaccatcaa 180
agaaggttaa tgtggctgtg gtttcagggt ccataaagct tttcaattca tctttttttt 240
ttttgttctt ttttttgatt ccggtttctt tgaaattttt ttgattcggt aatctccgag 300
cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtggt 360
gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc 420
aggaaacgaa gataaagcgg ccgcataact tcgtataatg tatgctatac gaagttatct 480
gccagtatac agctagcctt gaaagtgatg gaaaacattg tcatcggcac ataaataaaa 540
aaattatgaa tcacgtgatc aacagcaaat tatgtactcg tatatatgca agcgcattcc 600
ttatattgac actctttcat tgggcatgag gctgtgtaaa cataagctgt aacggtctca 660
cggaacactg tgtagttgca ttactgtcag gcagttatgt tgcttaatat aaaggcaaag 720
gcatggcaga atcactttaa aacgtggccc cacccgctgc accctgtgca ttttgtacgt 780
tactgcgaaa tgactcaacg atgaaatgaa aaaattttgc ttgaaatttt gaaaaaaaga 840
tgtgcgggac gcattgttag ctcattgaat acatcgtgat cgaatccaat caatgtttaa 900
tttcatatta atacagaaac tttttctcat actttcttct tcttttcatt ggtatattat 960
ctatatatcg tgttaattcc tctttcgtca tttttagcat cgttataaga gtaattaaga 1020
ataactagaa gagtctctct ttatattcgt ttattttata tatttaaccg ctaaatttag 1080
taaacaaaag aatctatcag aaatgagtga atctccaatg ttcgctgcca acggcatgcc 1140
aaaggtaaat caaggtgctg aagaagatgt cagaatttta ggttacgacc cattagcttc 1200
tccagctctc cttcaagtgc aaatcccagc cacaccaact tctttggaaa ctgccaagag 1260
aggtagaaga gaagctatag atattattac cggtaaagac gacagagttc ttgtcattgt 1320
cggtccttgt tccatccatg atctagaagc cgctcaagaa tacgctttga gattaaagaa 1380
attgtcagat gaattaaaag gtgatttatc catcattatg agagcatact tggagaagcc 1440
aagaacaacc gtcggctgga aaggtctaat taatgaccct gatgttaaca acactttcaa 1500
catcaacaag ggtttgcaat ccgctagaca attgtttgtc aacttgacaa atatcggttt 1560
gccaattggt tctgaaatgc ttgataccat ttctcctaaa tacttggctg atttggtctc 1620
cttcggtgcc attggtgcca gaaccaccga atctcaactg cacagagaat tggcctccgg 1680
tttgtctttc ccagttggtt tcaagaacgg taccgatggt accttaaatg ttgctgtgga 1740
tgcttgtcaa gccgctgctc attctcacca tttcatgggt gttactaagc atggtgttgc 1800
tgctatcacc actactaagg gtaacgaaca ctgcttcgtt attctaagag gtggtaaaaa 1860
gggtaccaac tacgacgcta agtccgttgc agaagctaag gctcaattgc ctgccggttc 1920
caacggtcta atgattgact actctcacgg taactccaat aaggatttca gaaaccaacc 1980
aaaggtcaat gacgttgttt gtgagcaaat cgctaacggt gaaaacgcca ttaccggtgt 2040
catgattgaa tcaaacatca acgaaggtaa ccaaggcatc ccagccgaag gtaaagccgg 2100
cttgaaatat ggtgtttcca tcactgatgc ttgtataggt tgggaaacta ctgaagacgt 2160
cttgaggaaa ttggctgctg ctgtcagaca aagaagagaa gttaacaaga aatagatgtt 2220
tttttaatga tatatgtaac gtacattctt tcctctacca ctgccaattc ggtattattt 2280
aattgtgttt agcgctattt actaattaac tagaaactca atttttaaag gcaaagctcg 2340
ctgacctttc actgatttcg tggatgttat actatcagtt actcttctgc aaaaaaaaat 2400
tgagtcatat cgtagctttg ggattatttt tctctctctc cacggctaat taggtgatca 2460
tgaaaaaatg aaaaattcat gagaaaagag tcagacatcg aaacatacat aagttgatat 2520
tcctttgata tcgacgacta ctcaatcagg ttttaaaaga aaagaggcag ctattgaagt 2580
agcagtatcc agtttaggtt ttttaattat ttacaagtaa agaaaaagag aatgccggtc 2640
gttcacgata acttcgtata atgtatgcta tacgaagtta tgcggccgcg agaagatgcg 2700
gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 2760
gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttctata 2820
atgacgaaaa aaaaaaaatt ggaaagaaaa agcttcatgg cctttataaa aaggaactat 2880
ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag 2940
acaggactgt aaagatggac gcattgaact ccaaagaaca acaagagttc caaaaagtag 3000
tggaacaaaa gcaaatgaag gatttcatgc gtttgtactc taatctggta gaaagatgtt 3060
tcacagactg tgtcaatgac ttcacaacat caaagctaac caataaggaa caaacatgca 3120
tcatgaagtg ctcagaaaag ttcttgaagc atagcgaacg tgtagggcag cgtttccaag 3180
ag 3182
<210> 2
<211> 3275
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和构巢曲霉。
<400> 2
cctactgcgc caattgatga caatacagac gatgataaca aaccgaagtt atctgatgta 60
gaaaaggatt aaagatgcta agagatagtg atgatatttc ataaataatg taattctata 120
tatgttaatt accttttttg cgaggcatat ttatggtgaa gaataagttt tgaccatcaa 180
agaaggttaa tgtggctgtg gtttcagggt ccataaagct tttcaattca tcattttttt 240
tttattcttt tttttgattc cggtttcctt gaaatttttt tgattcggta atctccgaac 300
agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg 360
ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaatctgca 420
ggaaacgaag ataaagcggc cgcataactt cgtatagcat acattatacg aagttatcgc 480
ctgttaagat ataactgaaa aaagagggga atttttagat actgaaatga tattttagaa 540
taaccagact atatataagg ataaattaca aaaaattaac taatagataa gatttaaata 600
taaaagatat gcaactagaa aagtcttatc aatctcctta tggagtgacg acgttaccca 660
acaatttacc gacttcttcg gcgatagcca aagttctctc ttcggacaat cttctaccaa 720
taacttgaac agcaacagga gcaccgtgat aagcctctgg gtcgtattct tcttgaacca 780
aagcatccaa ttcggaaaca gctttaaaag attcgttctt cttatcaata ttcttatcag 840
cgaaagtgac tgggacgaca acagaggtga aatccaataa gttaataacg gaggcgtaac 900
cgtagtatct gaattgatcg tgtctgacag cggcggtagg agtaattgga gcgataatag 960
cgtccaattc cttaccagct ttttcttcag cttcacgcca cttttccaag tattccattt 1020
gatagttcca cttttgtaaa tgagtgtccc acaattcgtt catgttaaca gccttaatat 1080
ttgggttcaa caagtcctta atgttaggga tggctggctc accagaggca gaaatgtctc 1140
tcatgacgtc ggcagaacca tcagcagcat agatgtggga aatcaagtca tgaccgaaat 1200
catgcttgta tggagtccat ggagtaacgg tgtgaccagc cttggccaaa gcggcaacgg 1260
tagtttcgac accacgtaaa attggtgggt gtggcaagac gttaccgtcg aaattgtaat 1320
aaccaatgtt caaaccacca ttcttaatct tagaggcaat gatgtcagat tcagattgtc 1380
tccatggcat tgggatgacc ttagagtcgt acttccaagg ttcttgaccc aagacagatt 1440
tggtgaacaa tctcaagtct tcgacggagt gagtgatagg accaacgacg gagtgaacgg 1500
tttcttgacc ttccatagag ttagccattt tagcatatgg caatctaccg tgagatggtc 1560
tcaaaccgta taaaaagttg aaagcagctg ggactctaat ggaaccacca atgtcagtac 1620
cgacaccaat aacaccacct ctaataccaa caatagcacc ttcaccacca gaagaaccac 1680
cacaggacca atttttgttt cttggattga cagttctacc aatgatgttg ttgacggttt 1740
cacagaccat caaggtttgt gggacagagg tcttaacgta gaaaacagca ccagcttttc 1800
tcaacatggt ggttaagacg gaatcacctt catcgtattt gtttaaccag gaaatgtaac 1860
ccatggaggt ttcgtaaccc ttaacacgca attggtcctt taaagagatt ggtaaaccgt 1920
gtaatggacc aactggtctc ttatgcttag cgtagtattc atctaattct ctagcttgag 1980
ctaaagcagc atctgggaag aattcgtgag cacagttggt taattgttga gcaatagcag 2040
ctctcttaca aaaagccaaa gtgacttcaa cagaagtcaa ctcaccagcg gccaacttgg 2100
agaccaaatc agcagcagag gcttcggtaa tcttcaattc agcctcagac aaaataccgg 2160
acttctttgg gaaatcaata acggaatctt cggcaggcaa agtttgaacc ttccattcgt 2220
caggaatggt tttagccaaa cgggcacgtt tgtcggcggc caattcttcc caggattgtg 2280
gcattttgta attaaaactt agattagatt gctatgcttt ctttctaatg agcaagaagt 2340
aaaaaaagtt gtaatagaac aagaaaaacg aaactgaaac ttgagaaatt gaagaccatt 2400
tattaactta aatatcaatg ggaggtcatc gaaagagaaa aaaatcaaaa aaaaaatttt 2460
tcaagaaaaa gaaacgtgat aaaaattttt attgcctttt tcgacgaaga aaaagaaacg 2520
aggcggtctc ttttttcttt tccaaacctt tagtacgggt aattaacgcc accctagagg 2580
aagaaagagg ggaaatttag tatgctgtgc ttgggtgttt tgaagtggta cggcgatgcg 2640
cggagtccga gaaaatctgg aagagtaaaa aaggagtaga aacattttga agctatggtg 2700
tgtgggggat cacttgtggg ggattgggtg tgatgtaagg ataacttcgt atagcataca 2760
ttatacgaag ttatgcggcc gcgagaagat gcggccagca aaactaaaaa actgtattat 2820
aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat 2880
tacccgggaa tctcggtcgt aatgattttt ataatgacga aaaaaaaaaa attggaaaga 2940
aaaagcttca tggcctttat aaaaaggaac catccaatac ctcgccagaa ccaagtaaca 3000
gtattttacg gggcacaaat caagaacaat aagacaggac tgtaaagatg gacgcattga 3060
actccaaaga acaacaagag ttccaaaaag tagtggaaca aaagcaaatg aaggatttca 3120
tgcgtttgta ctctaatctg gtagaaagat gttttacaga ctgtgtcaat gacttcacaa 3180
catcaaagct aaccaataag gaacaaacat gcatcatgaa gtgctcagaa aagttcttga 3240
agcatagcga acgtgtaggg cagcgtttcc aagag 3275
<210> 3
<211> 1132
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和p1噬菌体
<400> 3
ctctttttta cagatcatca aggaagtaat tatctacttt ttacaagaat tcatgtctaa 60
tttacttact gttcaccaaa acttgcctgc attaccagtt gacgcaacct ccgatgaagt 120
cagaaagaac cttatggata tgtttagaga tagacaagct ttctccgaac atacttggaa 180
aatgttatta tccgtttgta gatcctgggc cgcttggtgt aaacttaaca atagaaaatg 240
gtttcctgct gaaccagaag acgtcagaga ttacttactt tacttacaag ctagaggttt 300
ggctgttaaa actatccaac aacacttagg tcaattgaat atgttacaca gaagatccgg 360
tttaccaaga ccatccgatt ccaacgcagt ttcccttgtt atgagaagaa ttagaaaaga 420
aaatgttgac gctggtgaaa gagctaaaca agcattagca tttgaaagaa ccgatttcga 480
tcaagttaga tccttaatgg aaaattccga tagatgtcaa gatattagaa acttagcttt 540
cttaggtatt gcttacaaca cattattaag aatcgctgaa attgctagaa ttagagttaa 600
agatatttca agaaccgatg gcggtagaat gttaatccac attggcagaa caaaaacctt 660
agtctccaca gcaggcgtcg aaaaagcatt atcattaggt gttactaaat tagttgaacg 720
ttggatttcc gtttccggtg ttgcagatga cccaaacaac tacttattct gtcgtgttag 780
aaaaaatggt gttgccgctc cttccgctac ctcacaatta tccacaagag cattagaagg 840
catttttgaa gctacccaca gacttattta tggtgcaaaa gacgattccg gtcaaagata 900
tttagcttgg tctggtcatt ccgctagagt tggtgccgca agagacatgg caagagctgg 960
tgtttctatt cctgaaatta tgcaagccgg tggttggact aatgttaaca ttgttatgaa 1020
ctatatcaga aacttagatt ccgaaacagg tgctatggtt agattacttg aagacggtga 1080
ttaagctagc taagatccgc tctaaccgaa aaggaaggag ttagacaacc tg 1132
<210> 4
<211> 6376
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由多个单独的酿酒酵母序列组合在一起的序列
<400> 4
ctagctaaga tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc 60
tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct 120
tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa 180
ggttttggga cgctcgaaga tccagctgca ttaatgaatc ggccaacgcg cggggagagg 240
cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 300
tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 360
aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 420
aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 480
tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 540
ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 600
cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 660
ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 720
ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 780
gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 840
agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 900
cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 960
aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 1020
aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 1080
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 1140
aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 1200
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 1260
agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 1320
cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 1380
ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 1440
gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 1500
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 1560
cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 1620
ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 1680
catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 1740
tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 1800
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 1860
catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 1920
cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 1980
cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 2040
acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 2100
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 2160
tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag 2220
aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta 2280
cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt 2340
tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat 2400
ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc 2460
ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat 2520
tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta 2580
ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc 2640
ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga 2700
taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa 2760
agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg 2820
tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat 2880
gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa 2940
tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata 3000
gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg 3060
tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc 3120
gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat 3180
aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa 3240
cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt 3300
atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata 3360
tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc 3420
atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact 3480
cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact 3540
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 3600
gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 3660
tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 3720
gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 3780
tgcaccatac cacagctttt caattcaatt catcattttt tttttattct tttttttgat 3840
ttcggtttct ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga 3900
aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt 3960
gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca 4020
tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc 4080
tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca 4140
ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac 4200
atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat 4260
ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag 4320
tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg 4380
cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa 4440
caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta 4500
ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg 4560
gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga 4620
cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg 4680
atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg 4740
gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga 4800
gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact 4860
cacaaattag agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg 4920
cacagatgcg taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa 4980
aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 5040
aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 5100
acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 5160
agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 5220
gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 5280
cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 5340
caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 5400
agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 5460
ctcttcgcta ttacgccagc tgaattggag cgacctcatg ctatacctga gaaagcaacc 5520
tgacctacag gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac 5580
tttaaaattt gtatacactt atttttttta taacttattt aataataaaa atcataaatc 5640
ataagaaatt cgcttattta gaagtgtcaa caacgtatct accaacgatt tgaccctttt 5700
ccatcttttc gtaaatttct ggcaaggtag acaagccgac aaccttgatt ggagacttga 5760
ccaaacctct ggcgaagaat tgttaattaa gccagaaaaa ggaagtgttt ccctccttct 5820
tgaattgatg ttaccctcat aaagcacgtg gcctcttatc gagaaagaaa ttaccgtcgc 5880
tcgtgatttg tttgcaaaaa gaacaaaact gaaaaaaccc agacacgctc gacttcctgt 5940
cttcctattg attgcagctt ccaatttcgt cacacaacaa ggtcctagcg acggctcaca 6000
ggttttgtaa caagcaatcg aaggttctgg aatggcggga aagggtttag taccacatgc 6060
tatgatgccc actgtgatct ccagagcaaa gttcgttcga tcgtactgtt actctctctc 6120
tttcaaacag aattgtccga atcgtgtgac aacaacagcc tgttctcaca cactcttttc 6180
ttctaaccaa gggggtggtt tagtttagta gaacctcgtg aaacttacat ttacatatat 6240
ataaacttgc ataaattggt caatgcaaga aatacatatt tggtcttttc taattcgtag 6300
tttttcaagt tcttagatgc tttctttttc tcttttttac agatcatcaa ggaagtaatt 6360
atctactttt tacaag 6376
<210> 5
<211> 4632
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和扣囊复膜酵母
<400> 5
cagagcctct tatattcact ctgttcctcc atcgcctatt gagaaacgtt ggaataaaac 60
tctaaaaata tcatctagtt ggttagtttt tattttacca gtacattgtc acttgcggag 120
ggaggatgac ataaagattg agacgcagtc atttaatgaa gtttaaacgc aggtatttga 180
taaagtaata cgatattgaa tcatgacgta taaagtgaaa tgaacaaatg attacgtaaa 240
aaatgtcgat tttctcttga gagactccca tagcctctaa gaggccttct actacgttcc 300
atatatctaa gaatggggcc atatccagtg gaatcccagc aattatttaa ggatcaccta 360
tttctcagcc gatattttag caaaatcact accaatatca gggggcaata gttgatcgcc 420
tactttaaca aaaaatgttg ctcacgtatt aacacaggca acaaaaagga tattacgcaa 480
gaacgtagta tccacatgcc atcctccttg ttgcatcttt ttttttccga aatgattccc 540
tttcctgcac aacacgagat ctttcacgca tacatcggaa ggatcacccc ccactcaagt 600
cgttgcattg ctaacatgtg gcattctgcc catttttttc acgaaaattc tctctctata 660
atgaagaccc ttgtgccctg gactctgtaa tacttgaaac tacttcctca ataatcgctt 720
ggagacctac ccccacgctt ttcaaacaag gcgctagcaa aaagcctgcc gatatctcct 780
tgccccctcc ttctgttcga gagaactacg acccgaccaa taataatgtc atacaagaac 840
cgccaagaac caactgctga accttagatc tccaatactt cagttggagt atgtgaatat 900
ataagtacct ggtcgactaa tcttcttgca tcttttcgta ttcttacatc ctatgtcgct 960
aatacagttc ccgcatagag aagaaagcaa acaaaagtag tcactcgaga tctcccgagt 1020
ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc 1080
ctaactttat ttagtcaaaa aattggcctt ttaattctgc tgtaacccgt acatgcccaa 1140
aatagggggc gggttacaca gaatatataa catcataggt gtctgggtga acagtttatt 1200
cctggcatcc actaaatata atggagcccg ctttttttaa gctggcatcc agaaaaaaaa 1260
agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc 1320
ttagcgcaac tacacagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg 1380
agtgatgcaa cctgcttgga gtaaatgatg acacaaggca attgacctac gcatgtatct 1440
atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa 1500
aaaaaggttg aaaccagttc cctgaaatta ttcccctatt tgactaataa gtatataaag 1560
acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt 1620
tatagttagt ctttttttta gtttaaaaca ccaagaactt agtttcgaat aaacacacat 1680
aaacaaacaa atctagaatg attagattaa ccgtattcct cactgcagtt tttgcagcag 1740
tcgcttcctg tgttccagtt gaattggata agagaaatac aggccatttc caagcatatt 1800
ctggttacac cgtagctaga tcaaacttta ctcaatggat tcacgagcaa ccagccgtat 1860
catggtacta tttgcttcag aatatagact atccagaagg acaattcaag tctgccaagc 1920
caggggtcgt tgtggcttcc ccttctacat ccgaacctga ttacttctac caatggacta 1980
gagatactgc tatcaccttc ttgtcactta tcgcggaagt tgaggatcat tctttttcaa 2040
atactacact agccaaggtg gttgaatact acatctctaa tacttacaca ttacaaagag 2100
tttccaaccc atctggtaac ttcgacagtc caaatcacga cggtttggga gaaccaaagt 2160
ttaatgttga tgatacagct tatactgcat cttggggtag accacaaaat gatggcccag 2220
cgttgagagc atacgcaatt tcaagatacc ttaacgcagt agcaaaacac aacaacggta 2280
agttactgct cgctggacaa aacggtattc cttactcttc agcttctgat atctactgga 2340
agattatcaa gccagatctt caacatgtgt caacccattg gtctacatct ggttttgatt 2400
tgtgggaaga gaatcaggga acacatttct ttactgcgtt ggtccagcta aaagcactta 2460
gttacggcat tcctttaagt aagacctaca acgatcctgg tttcactagt tggctagaaa 2520
agcaaaagga tgctttaaac tcttatatca acagctctgg tttcgtaaac tctggcaaaa 2580
agcatatagt ggagagccct caactatctt caagaggagg gttggatagc gccacataca 2640
ttgcagcctt aatcacacat gatattggcg acgacgacac ttacacacct ttcaacgttg 2700
acaactccta tgtcttgaac tcactgtatt accttctagt cgataacaaa aaccgttaca 2760
aaatcaatgg taactacaag gccggtgctg ctgttggtag atacccagag gatgtttaca 2820
acggtgttgg gacatcagaa ggcaatccat ggcaattagc tacagcctac gccggccaaa 2880
cattttacac actggcttac aactcattga aaaacaaaaa aaacttagtg attgaaaagt 2940
tgaactacga cctctacaat tctttcatag cagatttatc caagatcgat agttcttacg 3000
catcaaaaga ctccttgact ttgacctacg gttctgacaa ctacaaaaac gtcataaagt 3060
cactattaca gtttggagat tcattcctga aggtcttgct cgatcacatt gatgataatg 3120
gacaattaac agaagagatc aatagataca cagggttcca ggctggtgct gttagtttga 3180
catggtcctc tggttcatta ctttcagcaa accgtgcgag aaataagttg attgaactat 3240
tgtagttaat taaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg 3300
cttacattca cgccctcctc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc 3360
tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat 3420
atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaacg ggcagacggc 3480
cggccataac ttcgtataat gtatgctata cgaagttatg gcaacggttc atcatctcat 3540
ggatctgcac atgaacaaac accagagtca aacgacgttg aaattgaggc tactgcgcca 3600
attgatgaca atacagacga tgataacaaa ccgaagttat ctgatgtaga aaaggattag 3660
agatgctaag agatagtgat gatatttcat aaataatgta attctatata tgttaattac 3720
cttttttgcg aggcatattt atggtgaagg ataagttttg accatcaaag aaggttaatg 3780
tggctgtggt ttcagggtcc ataaagcttt tcaattcatc tttttttttt ttgttctttt 3840
ttttgattcc ggtttctttg aaattttttt gattcggtaa tctccgagca gaaggaagaa 3900
cgaaggaagg agcacagact tagattggta tatatacgca tatgtggtgt tgaagaaaca 3960
tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga 4020
taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct 4080
gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt 4140
cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta 4200
aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag 4260
gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt 4320
aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt 4380
acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcggaa 4440
gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc 4500
ctagctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt 4560
gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg 4620
attatgacac gc 4632
<210> 6
<211> 4363
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和扣囊复膜酵母
<400> 6
ggccgctcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt 60
ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct 120
gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc 180
ccaggtattg ttagcggttt gaagcaggcg gcggaagaag taacaaagga acctagaggc 240
cttttgatgt tagcagaatt gtcatgcaag ggctccctag ctactggaga atatactaag 300
ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga 360
gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta 420
gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca 480
ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta 540
gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa 600
aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca 660
atttaattat atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa 720
aaaaaaaaaa ttggaaagaa aaagcttcat ggcctttata aaaaggaacc atccaatacc 780
tcgccagaac caagtaacag tattttacgg ggcacaaatc aagaacaata agacaggact 840
gtaaagatgg acgcattgaa ctccaaagaa caacaagagt tccaaaaagt agtggaacaa 900
aagcaaatga aggatttcat gcgtttgata acttcgtata atgtatgcta tacgaagtta 960
tctcgagggc cagaaaaagg aagtgtttcc ctccttcttg aattgatgtt accctcataa 1020
agcacgtggc ctcttatcga gaaagaaatt accgtcgctc gtgatttgtt tgcaaaaaga 1080
acaaaactga aaaaacccag acacgctcga cttcctgtct tcctgttgat tgcagcttcc 1140
aatttcgtca cacaacaagg tcctagcgac ggctcacagg ttttgtaaca agcaatcgaa 1200
ggttctggaa tggcgggaaa gggtttagta ccacatgcta tgatgcccac tgtgatctcc 1260
agagcaaagt tcgttcgatc gtactgttac tctctctctt tcaaacagaa ttgtccgaat 1320
cgtgtgacaa caacagcctg ttctcacaca ctcttttctt ctaaccaagg gggtggttta 1380
gtttagtaga acctcgtgaa acttacattt acatatatat aaacttgcat aaattggtca 1440
atgcaagaaa tacatatttg gtcttttcta attcgtagtt tttcaagttc ttagatgctt 1500
tctttttctc ttttttacag atcatcaagg aagtaattat ctacttttta caagtctaga 1560
atgatcagac ttacagtttt cctaacagcc gttttcgccg ccgttgcatc atgtgtccca 1620
gtagaattgg ataagagaaa caccggccat ttccaagcat attcaggata caccgttgca 1680
cgttctaatt tcacacaatg gattcatgag cagcctgctg tgtcctggta ctacttatta 1740
caaaacattg attatcctga gggacaattc aagtcagcga aaccaggcgt tgtggttgct 1800
tctccatcca cttcagaacc agactacttc taccagtgga cccgtgacac agcaataact 1860
ttcttatctt tgatagcaga agtagaagat cactcatttt caaatacaac tctagctaag 1920
gttgtcgaat actacatctc taacacatac accctacaaa gagtttctaa cccatctggt 1980
aatttcgata gcccaaatca cgatggtctg ggtgaaccaa agttcaacgt tgacgacact 2040
gcttacactg catcatgggg cagacctcaa aacgacggtc cagccttaag agcttacgcg 2100
atctcaagat atttgaacgc agttgccaag cataacaacg gtaagctatt gctcgcgggt 2160
caaaatggta ttccttactc atctgcatca gatatctact ggaagattat caagccagat 2220
ttacaacatg taagtactca ctggagtaca tctggttttg acttatggga agagaatcaa 2280
ggtacacatt tctttactgc acttgtccag ttaaaagctc tttcatacgg tatacctttg 2340
tctaagacat ataacgatcc aggatttact tcttggttgg aaaagcagaa ggatgccttg 2400
aactcttaca tcaattccag cggcttcgtc aactccggga aaaagcacat tgtcgaatct 2460
cctcaattat ctagtagagg gggtcttgat agcgctactt acatcgctgc tctaattaca 2520
catgatattg gtgatgatga tacatacact ccttttaacg tagataattc ttatgtgctg 2580
aactctttat actatctgct tgtagacaac aaaaacagat acaagatcaa cgggaactac 2640
aaagcaggag ctgcagttgg tagataccca gaagatgtgt acaatggagt gggaacctca 2700
gagggaaacc catggcaatt ggcgacagca tacgccggcc aaacctttta cacactggct 2760
tacaattctc tcaaaaacaa aaaaaatttg gttattgaga agttgaatta cgatctatac 2820
aactccttta tagctgactt aagtaagatt gactcctctt acgcttctaa ggattcattg 2880
acattgacct acggctcaga taactacaaa aatgtcatta agtcactttt acaattcggg 2940
gattctttct tgaaagtctt gttggaccat attgatgata atggtcagct aacagaggaa 3000
atcaacagat atacaggttt tcaagctggc gcagtttccc tcacttggag tagtggttca 3060
ctcttatctg caaacagagc cagaaacaag ttgatcgaat tgctttagtt aattaagaag 3120
ttttgttaga aaataaatca ttttttaatt gagcattctt attcctattt tatttaaata 3180
gttttatgta ttgttagcta catacaacag tttaaatcaa attttctttt tcccaagtcc 3240
aaaatggagg tttattttga tgacccgcat gcgattatgt tttgaaagta taagactaca 3300
tacatgtaca tatatttaaa catgtaaacc cgtccattat attgccgggc agacggccgg 3360
ccttatagcc tagctttaag gctactttaa aaacttttta tttattcata cacatatatt 3420
atcgaacatt cgtataactt aatatcattc aaaaaaaaaa aaaaaaaaaa aagaaaacat 3480
atacacatat atatttatgt ttatagagag agagagagaa aatttgaatt tttgaatcat 3540
ttgcaaagtt atatgtttta tacattattt attcattttt tttggtgtcg aggacattgt 3600
gctgttcaga gaaccactta aaatacgcat cgttctgtaa atatccactt tcattaaaaa 3660
ccttattcac ttctaacttt gccttcaact ccttcttgga gttttctccc ttttttttct 3720
gaacaagctc aaccagatat aatggttcgt tcttttcgaa ctttgtcttt acatatattt 3780
cctcctttgt acctcttctc tttcccacat aaacagtccc cttttcaata aaacgagaga 3840
aataccagaa aagtagcgag agaacaaaat atgcgcctac caaaagcttt tgatacgtaa 3900
caatctgatc tctctcaaat tttttatcca agaagaaact caaaccagct acaacagcta 3960
tggaataacc tatgtacaat ttagcatcga gtaaagcgta tgatctctcg taatttaatc 4020
tcgcgaaaac agaaggtagg gcttcatcta aagcttggtt caactccggg attgaatata 4080
cattaatagg tttagcagaa ctcatcttga acaggcgtct cttttcctta caataacttg 4140
tgcttttcct tctataattc cgtttcaacg tgtacaattg tcattttttg tctggtatga 4200
ttttgcagaa ctgaaaaaat ctcttaaatg ttccgcctca tcaagaaggc atattccttt 4260
acaaaagtac attgatctta caagaagcta gctaatggta ctatttaaaa aacaactaca 4320
ctccatcaat acataaaatt gttatgatag acttgaggga cgg 4363
<210> 7
<211> 5015
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、扣囊复膜酵母和构巢曲霉
<400> 7
cagagcctct tatattcact ctgttcctcc atcgcctatt gagaaacgtt ggaataaaac 60
tctaaaaata tcatctagtt ggttagtttt tattttacca gtacattgtc acttgcggag 120
ggaggatgac ataaagattg agacgcagtc atttaatgaa gtttaaacgc aggtatttga 180
taaagtaata cgatattgaa tcatgacgta taaagtgaaa tgaacaaatg attacgtaaa 240
aaatgtcgat tttctcttga gagactccca tagcctctaa gaggccttct actacgttcc 300
atatatctaa gaatggggcc atatccagtg gaatcccagc aattatttaa ggatcaccta 360
tttctcagcc gatattttag caaaatcact accaatatca gggggcaata gttgatcgcc 420
tactttaaca aaaaatgttg ctcacgtatt aacacaggca acaaaaagga tattacgcaa 480
gaacgtagta tccacatgcc atcctccttg ttgcatcttt ttttttccga aatgattccc 540
tttcctgcac aacacgagat ctttcacgca tacatcggaa ggatcacccc ccactcaagt 600
cgttgcattg ctaacatgtg gcattctgcc catttttttc acgaaaattc tctctctata 660
atgaagaccc ttgtgccctg gactctgtaa tacttgaaac tacttcctca ataatcgctt 720
ggagacctac ccccacgctt ttcaaacaag gcgctagcaa aaagcctgcc gatatctcct 780
tgccccctcc ttctgttcga gagaactacg acccgaccaa taataatgtc atacaagaac 840
cgccaagaac caactgctga accttagatc tccaatactt cagttggagt atgtgaatat 900
ataagtacct ggtcgactaa tcttcttgca tcttttcgta ttcttacatc ctatgtcgct 960
aatacagttc ccgcatagag aagaaagcaa acaaaagtag tcactcgaga tctcccgagt 1020
ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc 1080
ctaactttat ttagtcaaaa aattggcctt ttaattctgc tgtaacccgt acatgcccaa 1140
aatagggggc gggttacaca gaatatataa catcataggt gtctgggtga acagtttatt 1200
cctggcatcc actaaatata atggagcccg ctttttttaa gctggcatcc agaaaaaaaa 1260
agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc 1320
ttagcgcaac tacacagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg 1380
agtgatgcaa cctgcttgga gtaaatgatg acacaaggca attgacctac gcatgtatct 1440
atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa 1500
aaaaaggttg aaaccagttc cctgaaatta ttcccctatt tgactaataa gtatataaag 1560
acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt 1620
tatagttagt ctttttttta gtttaaaaca ccaagaactt agtttcgaat aaacacacat 1680
aaacaaacaa atctagaatg atcagactta ctgttttcct cacagccgtt tttgcagcag 1740
tagcttcttg tgttccagtt gaattggata agagaaatac aggtcatttc caagcttact 1800
ctggttacac tgtggctaga tctaacttca cacaatggat tcatgaacag cctgccgtga 1860
gttggtacta tttgctacaa aacattgatt accctgaggg tcaattcaaa tcagctaagc 1920
caggtgttgt tgtcgcgagc ccatcaactt ctgaaccaga ttacttctac caatggacta 1980
gagataccgc aataaccttc ttatctctaa tcgcagaggt agaagatcac tctttttcaa 2040
atactaccct ggcaaaagtg gtcgagtact acatctcaaa cacatacacc ttgcagagag 2100
tctcaaaccc atcaggaaac ttcgattctc ctaatcatga cggcttagga gaaccaaagt 2160
ttaatgttga cgataccgct tatactgcat cttggggtag accacagaat gatggccctg 2220
ccttacgtgc atacgccatt tccagatatc tcaacgctgt agcgaagcac aacaacggta 2280
agctgctttt agctggtcaa aatgggatac catactcttc cgcttcagac atttactgga 2340
agattatcaa accagacttg cagcatgtca gtacacattg gtcaacttct ggttttgatt 2400
tgtgggaaga gaaccaaggc actcacttct ttacagcctt ggttcaacta aaggcattgt 2460
cttacggaat ccctttgtcc aagacataca atgatcctgg attcactagt tggctagaaa 2520
agcaaaagga tgcactgaac tcatacatta acagttcagg ctttgtgaac tccggtaaaa 2580
agcatattgt tgaaagccca caactatcta gcagaggtgg tttagattct gcaacctaca 2640
tagcagcctt gatcacacac gacattgggg atgacgatac atacacacca ttcaacgtcg 2700
acaattcata cgttttgaat agcttatact acctactggt agataacaaa aacagatata 2760
agatcaatgg caactacaag gccggtgctg ccgtaggaag ataccctgaa gatgtctaca 2820
acggagttgg tacatcagaa ggtaacccat ggcaattagc aacagcatat gcgggccaga 2880
cattttacac tttggcttac aattcattga aaaacaaaaa aaatttagtg atagaaaagc 2940
ttaactatga cctttacaac tctttcattg ccgatttatc caagattgat tcctcctacg 3000
catcaaagga ctccttgaca cttacatacg gttctgacaa ctacaaaaat gttatcaagt 3060
ctctcttgca atttggtgat tctttcttga aggttttact cgatcatatc gatgataatg 3120
gtcaactaac tgaggaaatc aacagataca ctgggttcca agctggagct gtctctttaa 3180
catggagttc agggagtttg ttatctgcta acagagcgcg taacaaactt attgagcttc 3240
tgtagttaat taaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg 3300
cttacattca cgccctcctc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc 3360
tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat 3420
atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaacg ggcagacggc 3480
cggccataac ttcgtataat gtatgctata cgaagttatc cttacatcac acccaatccc 3540
ccacaagtga tcccccacac accatagctt caaaatgttt ctactccttt tttactcttc 3600
cagattttct cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac 3660
taaatttccc ctctttcttc ctctagggtg gcgttaatta cccgtactaa aggtttggaa 3720
aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta 3780
tcacgtttct ttttcttgaa aaattttttt tttgattttt ttctctttcg atgacctccc 3840
attgatattt aagttaataa atggtcttca atttctcaag tttcagtttc gtttttcttg 3900
ttctattaca acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta 3960
agttttaatt acaaaatgcc acaatcctgg gaagaattgg ccgccgacaa acgtgcccgt 4020
ttggctaaaa ccattcctga cgaatggaag gttcaaactt tgcctgccga agattccgtt 4080
attgatttcc caaagaagtc cggtattttg tctgaggctg aattgaagat taccgaagcc 4140
tctgctgctg atttggtctc caagttggcc gctggtgagt tgacttctgt tgaagtcact 4200
ttggcttttt gtaagagagc tgctattgct caacaattaa ccaactgtgc tcacgaattc 4260
ttcccagatg ctgctttagc tcaagctaga gaattagatg aatactacgc taagcataag 4320
agaccagttg gtccattaca cggtttacca atctctttaa aggaccaatt gcgtgttaag 4380
ggttacgaaa cctccatggg ttacatttcc tggttaaaca aatacgatga aggtgattcc 4440
gtcttaacca ccatgttgag aaaagctggt gctgttttct acgttaagac ctctgtccca 4500
caaaccttga tggtctgtga aaccgtcaac aacatcattg gtagaactgt caatccaaga 4560
aacaaaaatt ggtcctgtgg tggttcttct ggtggtgaag gtgctattgt tggtattaga 4620
ggtggtgtta ttggtgtcgg tactgacatt ggtggttcca ttagagtccc agctgctttc 4680
aactttttat acggtttgag accatctcac ggtagattgc catatgctaa aatggctaac 4740
tctatggaag gtcaagaaac cgttcactcc gtcgttggtc ctatcactca ctccgtcgaa 4800
gacttgagat tgttcaccaa atctgtcttg ggtcaagaac cttggaagta cgactctaag 4860
gtcatcccca tgccatggag acaatctgaa tctgacatca ttgcctctaa gattaagaat 4920
ggtggtttga acattggtta ttacaatttc gacggtaacg tcttgccaca cccaccaatt 4980
ttacgtggtg tcgaaactac cgttgccgct ttggc 5015
<210> 8
<211> 4771
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、扣囊复膜酵母和构巢曲霉
<400> 8
ggccgcgaag gtgctattgt tggtattaga ggtggtgtta ttggtgtcgg tactgacatt 60
ggtggttcca ttagagtccc agctgctttc aactttttat acggtttgag accatctcac 120
ggtagattgc catatgctaa aatggctaac tctatggaag gtcaagaaac cgttcactcc 180
gtcgttggtc ctatcactca ctccgtcgaa gacttgagat tgttcaccaa atctgtcttg 240
ggtcaagaac cttggaagta cgactctaag gtcatcccaa tgccatggag acaatctgaa 300
tctgacatca ttgcctctaa gattaagaat ggtggtttga acattggtta ttacaatttc 360
gacggtaacg tcttgccaca cccaccaatt ttacgtggtg tcgaaactac cgttgccgct 420
ttggccaagg ctggtcacac cgttactcca tggactccat acaagcatga tttcggtcat 480
gacttgattt cccacatcta tgctgctgat ggttctgccg acgtcatgag agacatttct 540
gcctctggtg agccagccat ccctaacatt aaggacttgt tgaacccaaa tattaaggct 600
gttaacatga acgaattgtg ggacactcat ttacaaaagt ggaactatca aatggaatac 660
ttggaaaagt ggcgtgaagc tgaagaaaaa gctggtaagg aattggacgc tattatcgct 720
ccaattactc ctaccgccgc tgtcagacac gatcaattca gatactacgg ttacgcctcc 780
gttattaact tattggattt cacctctgtt gtcgtcccag tcactttcgc tgataagaat 840
attgataaga agaacgaatc ttttaaagct gtttccgaat tggatgcttt ggttcaagaa 900
gaatacgacc cagaggctta tcacggtgct cctgttgctg ttcaagttat tggtagaaga 960
ttgtccgaag agagaacttt ggctatcgcc gaagaagtcg gtaaattgtt gggtaacgtc 1020
gtcactccat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa 1080
aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc 1140
ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt 1200
attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcacc 1260
caattgtaga tatgctaact ccagcaatga gttgatgaat ctcggtgtgt attttatgtc 1320
ctcagaggac aacacataac ttcgtataat gtatgctata cgaagttatc tcgagggcca 1380
gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac cctcataaag cacgtggcct 1440
cttatcgaga aagaaattac cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa 1500
aaacccagac acgctcgact tcctgtcttc ctgttgattg cagcttccaa tttcgtcaca 1560
caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg 1620
gcgggaaagg gtttagtacc acatgctatg atgcccactg tgatctccag agcaaagttc 1680
gttcgatcgt actgttactc tctctctttc aaacagaatt gtccgaatcg tgtgacaaca 1740
acagcctgtt ctcacacact cttttcttct aaccaagggg gtggtttagt ttagtagaac 1800
ctcgtgaaac ttacatttac atatatataa acttgcataa attggtcaat gcaagaaata 1860
catatttggt cttttctaat tcgtagtttt tcaagttctt agatgctttc tttttctctt 1920
ttttacagat catcaaggaa gtaattatct actttttaca agtctagaat gattagatta 1980
acagtatttc ttacagccgt tttcgcagcc gtcgcatcct gtgttccagt agaattagat 2040
aagcgtaata caggacattt tcaagcttac tctggctata cagttgcgag atctaacttt 2100
acacaatgga ttcacgaaca gccagcagtt tcttggtact atttgctcca aaacatcgac 2160
taccctgaag gccaattcaa gtctgcaaag ccaggagtgg tcgtcgcttc tcctagtact 2220
tcagaaccag attacttcta ccagtggaca agagacactg ctattacctt cctgagctta 2280
atcgctgaag ttgaagatca ctctttttct aatacaacac tggccaaagt agttgagtac 2340
tacatctcta acacttacac tctacaaaga gtgtcaaacc cttctgggaa cttcgacagc 2400
ccaaaccatg atggtttggg ggagccaaaa ttcaacgttg atgatacagc ctacaccgca 2460
tcttggggta gaccacaaaa cgacggacca gctttaagag catacgcaat atctcgttac 2520
cttaatgctg ttgcaaagca caataatgga aagttgttgt tggctggtca aaacggtatt 2580
ccttactctt cagcatctga tatctactgg aagattatca agccagatct tcaacacgta 2640
tccacacatt ggtcaacctc cggcttcgat ttatgggagg aaaatcaggg tacacatttc 2700
ttcaccgctc tagtgcaatt gaaggctttg agttacggca ttccattgtc taagacttac 2760
aacgatcctg gtttcacctc atggcttgaa aagcagaagg atgccctgaa tagctacatc 2820
aactcatctg gttttgttaa ctcagggaaa aagcatatag ttgaatcccc acaactatca 2880
tcaagaggag gtttagactc cgccacatac attgctgcct tgattacaca tgatattggg 2940
gatgatgaca catatactcc atttaacgtc gataacagtt atgtccttaa ttccttatac 3000
tatttgttgg tcgataacaa aaatagatac aaaatcaacg gcaactacaa ggctggcgca 3060
gcggtgggta gataccctga ggatgtttac aatggtgtag gtacatctga aggcaatcca 3120
tggcaattag cgactgctta cgctggacaa actttctaca cacttgcgta caactcattg 3180
aaaaacaaaa aaaacctagt cattgaaaag ttgaattacg atctgtacaa ctctttcatc 3240
gcagacctat caaagattga ctcatcttat gcaagtaaag attcactaac tttaacctac 3300
ggtagtgata actacaaaaa cgttatcaag tctttactcc agtttggtga ttcattcttg 3360
aaggtgttgt tagatcatat agacgacaat ggtcaactca cagaggagat aaacagatac 3420
actggttttc aagcaggagc tgtttcactt acttggtcaa gtggttcttt gctttccgcc 3480
aacagagcca gaaacaagct catcgaatta ctatagttaa ttaagaagtt ttgttagaaa 3540
ataaatcatt ttttaattga gcattcttat tcctatttta tttaaatagt tttatgtatt 3600
gttagctaca tacaacagtt taaatcaaat tttctttttc ccaagtccaa aatggaggtt 3660
tattttgatg acccgcatgc gattatgttt tgaaagtata agactacata catgtacata 3720
tatttaaaca tgtaaacccg tccattatat tgccgggcag acggccggcc ttatagccta 3780
gctttaaggc tactttaaaa actttttatt tattcataca catatattat cgaacattcg 3840
tataacttaa tatcattcaa aaaaaaaaaa aaaaaaaaaa gaaaacatat acacatatat 3900
atttatgttt atagagagag agagagaaaa tttgaatttt tgaatcattt gcaaagttat 3960
atgttttata cattatttat tcattttttt tggtgtcgag gacattgtgc tgttcagaga 4020
accacttaaa atacgcatcg ttctgtaaat atccactttc attaaaaacc ttattcactt 4080
ctaactttgc cttcaactcc ttcttggagt tttctccctt ttttttctga acaagctcaa 4140
ccagatataa tggttcgttc ttttcgaact ttgtctttac atatatttcc tcctttgtac 4200
ctcttctctt tcccacataa acagtcccct tttcaataaa acgagagaaa taccagaaaa 4260
gtagcgagag aacaaaatat gcgcctacca aaagcttttg atacgtaaca atctgatctc 4320
tctcaaattt tttatccaag aagaaactca aaccagctac aacagctatg gaataaccta 4380
tgtacaattt agcatcgagt aaagcgtatg atctctcgta atttaatctc gcgaaaacag 4440
aaggtagggc ttcatctaaa gcttggttca actccgggat tgaatataca ttaataggtt 4500
tagcagaact catcttgaac aggcgtctct tttccttaca ataacttgtg cttttccttc 4560
tataattccg tttcaacgtg tacaattgtc attttttgtc tggtatgatt ttgcagaact 4620
gaaaaaatct cttaaatgtt ccgcctcatc aagaaggcat attcctttac aaaagtacat 4680
tgatcttaca agaagctagc taatggtact atttaaaaaa caactacact ccatcaatac 4740
ataaaattgt tatgatagac ttgagggacg g 4771
<210> 9
<211> 8719
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和p1噬菌体
<400> 9
atcacatagg aagcaacagg cgcgttggac ttttaatttt cgaggaccgc gaatccttac 60
atcacaccca atcccccaca agtgatcccc cacacaccat agcttcaaaa tgtttctact 120
ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac 180
ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt 240
actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg 300
caataaaaat ttttatcacg tttctttttc ttgaaaattt ttttttttga tttttttctc 360
tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc tcaagtttca 420
gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag aaagaaagca 480
tagcaatcta atctaagttt taattacaaa tctagaatga gtgaatctcc aatgttcgct 540
gccaacggca tgccaaaggt aaatcaaggt gctgaagaag atgtcagaat tttaggttac 600
gacccattag cttctccagc tctccttcaa gtgcaaatcc cagccacacc aacttctttg 660
gaaactgcca agagaggtag aagagaagct atagatatta ttaccggtaa agacgacaga 720
gttcttgtca ttgtcggtcc ttgttccatc catgatcttg aagccgctca agaatacgct 780
ttgagattaa agaaattgtc agatgaatta aaaggtgatt tatccatcat tatgagagca 840
tacttggaga agccaagaac aaccgtcggc tggaaaggtc taattaatga ccctgatgtt 900
aacaacactt tcaacatcaa caagggtttg caatccgcta gacaattgtt tgtcaacttg 960
acaaatatcg gtttgccaat tggttctgaa atgcttgata ccatttctcc taaatacttg 1020
gctgatttgg tctccttcgg tgccattggt gccagaacca ccgaatctca actgcacaga 1080
gaattggcct ccggtttgtc tttcccagtt ggtttcaaga acggtaccga tggtacctta 1140
aatgttgctg tggatgcttg tcaagccgct gctcattctc accatttcat gggtgttact 1200
aagcatggtg ttgctgctat caccactact aagggtaacg aacactgctt cgttattcta 1260
agaggtggta aaaagggtac caactacgac gctaagtccg ttgcagaagc taaggctcaa 1320
ttgcctgccg gttccaacgg tctaatgatt gactactctc acggtaactc caataaggat 1380
ttcagaaacc aaccaaaggt caatgacgtt gtttgtgagc aaatcgctaa cggtgaaaac 1440
gccattaccg gtgtcatgat tgaatcaaac atcaacgaag gtaaccaagg catcccagcc 1500
gaaggtaaag ccggcttgaa atatggtgtt tccatcactg atgcttgtat aggttgggaa 1560
actactgaag acgtcttgag gaaattggct gctgctgtca gacaaagaag agaagttaac 1620
aagaaataga tgttttttta atgatatatg taacgtacat tctttcctct accactgcca 1680
attcggtatt atttaattgt gtttagcgct atttactaat taactagaaa ctcaattttt 1740
aaaggcaaag ctcgctgacc tttcactgat ttcgtggatg ttatactatc agttactctt 1800
ctgcaaaaaa aaattgagtc atatcgtagc tttgggatta tttttctctc tctccacggc 1860
taattaggtg atcatgaaaa aatgaaaaat tcatgagaaa agagtcagac atcgaaacat 1920
acataagttg atattccttt gatatcgacg actactcaat caggttttaa aagaaaagag 1980
gcagctattg aagtagcagt atccagttta ggttttttaa ttatttacaa gtaaagaaaa 2040
agagaatgcc ggtcgttcac ggcggccgcg ccagaaaaag gaagtgtttc cctccttctt 2100
gaattgatgt taccctcata aagcacgtgg cctcttatcg agaaagaaat taccgtcgct 2160
cgtgatttgt ttgcaaaaag aacaaaactg aaaaaaccca gacacgctcg acttcctgtc 2220
ttcctattga ttgcagcttc caatttcgtc acacaacaag gtcctagcga cggctcacag 2280
gttttgtaac aagcaatcga aggttctgga atggcgggaa agggtttagt accacatgct 2340
atgatgccca ctgtgatctc cagagcaaag ttcgttcgat cgtactgtta ctctctctct 2400
ttcaaacaga attgtccgaa tcgtgtgaca acaacagcct gttctcacac actcttttct 2460
tctaaccaag ggggtggttt agtttagtag aacctcgtga aacttacatt tacatatata 2520
taaacttgca taaattggtc aatgcaagaa atacatattt ggtcttttct aattcgtagt 2580
ttttcaagtt cttagatgct ttctttttct cttttttaca gatcatcaac tcttttttac 2640
agatcatcaa ggaagtaatt atctactttt tacaagaatt catgtctaat ttacttactg 2700
ttcaccaaaa cttgcctgca ttaccagttg acgcaacctc cgatgaagtc agaaagaacc 2760
ttatggatat gtttagagat agacaagctt tctccgaaca tacttggaaa atgttattat 2820
ccgtttgtag atcctgggcc gcttggtgta aacttaacaa tagaaaatgg tttcctgctg 2880
aaccagaaga cgtcagagat tacttacttt acttacaagc tagaggtttg gctgttaaaa 2940
ctatccaaca acacttaggt caattgaata tgttacacag aagatccggt ttaccaagac 3000
catccgattc caacgcagtt tcccttgtta tgagaagaat tagaaaagaa aatgttgacg 3060
ctggtgaaag agctaaacaa gcattagcat ttgaaagaac cgatttcgat caagttagat 3120
ccttaatgga aaattccgat agatgtcaag atattagaaa cttagctttc ttaggtattg 3180
cttacaacac attattaaga atcgctgaaa ttgctagaat tagagttaaa gatatttcaa 3240
gaaccgatgg cggtagaatg ttaatccaca ttggcagaac aaaaacctta gtctccacag 3300
caggcgtcga aaaagcatta tcattaggtg ttactaaatt agttgaacgt tggatttccg 3360
tttccggtgt tgcagatgac ccaaacaact acttattctg tcgtgttaga aaaaatggtg 3420
ttgccgctcc ttccgctacc tcacaattat ccacaagagc attagaaggc atttttgaag 3480
ctacccacag acttatttat ggtgcaaaag acgattccgg tcaaagatat ttagcttggt 3540
ctggtcattc cgctagagtt ggtgccgcaa gagacatggc aagagctggt gtttctattc 3600
ctgaaattat gcaagccggt ggttggacta atgttaacat tgttatgaac tatatcagaa 3660
acttagattc cgaaacaggt gctatggtta gattacttga agacggtgat taagctagct 3720
aagatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta 3780
tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt 3840
tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt 3900
gggacgctcg aaggagctcc aattcgccct atagtgagtc gtattacaat tcactggccg 3960
tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 4020
cacatccccc cttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 4080
aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg 4140
cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 4200
ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 4260
taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 4320
aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 4380
ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 4440
tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 4500
ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt 4560
ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcag 4620
ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt 4680
acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct 4740
gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt 4800
ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga 4860
cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa 4920
ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg 4980
ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat 5040
gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc 5100
ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat 5160
tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca 5220
aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta 5280
actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt 5340
ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa 5400
gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta 5460
ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg 5520
caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca 5580
ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt 5640
tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa 5700
aaaaatgatg aattgaattg aaaagcgtgg tgcactctca gtacaatctg ctctgatgcc 5760
gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 5820
ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 5880
aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 5940
ttataggtta atgtcatgat aataatggtt tcttaggacg gatcgcttgc ctgtaactta 6000
cacgcgcctc gtatctttta atgatggaat aatttgggaa tttactctgt gtttatttat 6060
ttttatgttt tgtatttgga ttttagaaag taaataaaga aggtagaaga gttacggaat 6120
gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata 6180
tatttattag acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa 6240
tgtaaaatca caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc 6300
attaatacct gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta 6360
gagtctttta catcttcgga aaacaaaaac tattttttct ttaatttctt tttttacttt 6420
ctatttttaa tttatatatt tatattaaaa aatttaaatt ataattattt ttatagcacg 6480
tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 6540
atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 6600
tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 6660
cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 6720
agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 6780
taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 6840
tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 6900
catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 6960
ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 7020
ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt tttttcacaa 7080
catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 7140
aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 7200
aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 7260
taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 7320
atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 7380
gccctcccgt atcgtagtta tctacacgac gggcagtcag gcaactatgg atgaacgaaa 7440
tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 7500
ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 7560
gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 7620
agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 7680
aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 7740
agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 7800
tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 7860
atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 7920
taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 7980
gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 8040
gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 8100
aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggga acgcctggta 8160
tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 8220
gtcagggggg ccgagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 8280
cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 8340
ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 8400
cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 8460
ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 8520
gcgcaacgca attaatgtga gttacctcac tcattaggca ccccaggctt tacactttat 8580
gcttccggct cctatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 8640
ctatgaccat gattacgcca agctcggaat taaccctcac taaagggaac aaaagctggg 8700
taccgggccc cccctcgag 8719
<210> 10
<211> 8086
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和麦卡特酵母
<400> 10
ccgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa 60
cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaga 120
cagaggagag cataaaaatg gggttcactt tttggtaaag ctatagcatg cctatcacat 180
ataaatagag tgccagtagc gacttttttc acactcgaaa tactcttact actgctctct 240
tgttgttttt atcacttctt gtttcttctt ggtaaataga atatcaagct acaaaaagca 300
tacctcgagg ccagaaaaag gaagtgtttc cctccttctt gaattgatgt taccctcata 360
aagcacgtgg cctcttatcg agaaagaaat taccgtcgct cgtgatttgt ttgcaaaaag 420
aacaaaactg aaaaaaccca gacacgctcg acttcctgtc ttcctgttga ttgcagcttc 480
caatttcgtc acacaacaag gtcctagcga cggctcacag gttttgtaac aagcaatcga 540
aggttctgga atggcgggaa agggtttagt accacatgct atgatgccca ctgtgatctc 600
cagagcaaag ttcgttcgat cgtactgtta ctctctctct ttcaaacaga attgtccgaa 660
tcgtgtgaca acaacagcct gttctcacac actcttttct tctaaccaag ggggtggttt 720
agtttagtag aacctcgtga aacttacatt tacatatata taaacttgca taaattggtc 780
aatgcaagaa atacatattt ggtcttttct aattcgtagt ttttcaagtt cttagatgct 840
ttctttttct cttttttaca gatcatcaag gaagtaatta tctacttttt acaagtctag 900
aatgactatc tcttctgctc acccagaaac tgaaccaaag tggtggaaag aggcaacttt 960
ttaccaaatc tacccagctt cattcaagga ctccaatgat gatggttggg gtgatatgaa 1020
aggtattgct tccaaattag aatacattaa ggaattaggt gccgatgcta tttggatttc 1080
tccattctat gattctccac aagacgatat gggttatgac atcgctaact atgaaaaggt 1140
ttggccaacc tatggcacta atgaggactg ttttgcatta attgagaaaa cccacaagtt 1200
gggcatgaag ttcattactg atcttgtcat taatcattgt tcatccgaac atgaatggtt 1260
caaggaatcc agatcctcca aaactaatcc aaaaagagat tggtttttct ggagaccacc 1320
taagggttat gatgctgaag gtaagccaat tccaccaaac aattggaagt cttactttgg 1380
tggttccgca tggaccttcg acgaaaagac ccaagagttt tacttgagat tattctgctc 1440
cacccaacca gatttgaact gggaaaatga agattgtaga aaagcaatct acgaatctgc 1500
agttggctat tggttagatc acggtgttga tggtttcaga attgatgttg gttcacttta 1560
ctcaaaggtt gttggtttgc cagatgcacc agttgttgat aaaaactcta catggcaatc 1620
ttctgaccca tacactctta atggtcctag aatccatgaa tttcatcaag agatgaacca 1680
gttcattaga aatagagtta aggatggtag agaaattatg accgttggtg aaatgcaaca 1740
tgcatctgat gaaactaaga gattatacac atcagcctcc cgtcacgaat tgtctgaatt 1800
attcaacttt tcacacacag acgttggcac atccccatta ttccgttata acttggttcc 1860
attcgaattg aaggactgga aaatcgcatt ggcagaattg tttagatata tcaatggtac 1920
tgattgttgg tctaccatct acttggaaaa ccacgaccaa ccaagatcca tcactagatt 1980
cggtgatgac tctcctaaaa accgtgtcat ttctggtaag ttactttctg tcttattatc 2040
cgccttaacc ggtactttgt acgtctatca aggccaggaa ttgggtcaaa ttaactttaa 2100
gaattggcca gtcgaaaagt atgaagatgt cgaaatcaga aacaactaca atgcaattaa 2160
ggaggaacat ggtgaaaatt cagaggaaat gaaaaagttt ttggaagcta ttgctcttat 2220
ttccagagat cacgctagaa ccccaatgca atggtcaaga gaggaaccta acgctggttt 2280
ctctggtcct tccgccaagc cttggtttta cttaaacgac tccttcagag aaggtattaa 2340
cgttgaagat gaaattaagg acccaaattc cgtccttaac ttctggaagg aagcattgaa 2400
gtttagaaag gcccataagg atattaccgt ttatggttat gactttgagt ttatcgattt 2460
ggataacaaa aagttattct cattcactaa aaagtataac aacaagacct tattcgctgc 2520
tttaaacttc tcttctgatg ctactgattt caaaattcct aatgacgatt cctctttcaa 2580
gttggagttt ggtaactacc caaagaagga agttgacgca tcttctcgta cattgaagcc 2640
ttgggaaggt agaatctaca tctccgagta acctgcaggt ttgccagctt actatccttc 2700
ttgaaaatat gcactctata tcttttagtt cttaattgca acacatagat ttgctgtata 2760
acgaatttta tgctattttt taaatttgga gttcagtgat aaaagtgtca cagcgaattt 2820
cctcacatgt agggaccgaa ttgtttacaa gttctctgta ccaccatgga gacatcaaaa 2880
attgaaaatc tatggaaaga tatggacggt agcaacaaga atatagcacg agccggcgac 2940
tagtaacggc cgccagtgtg ctggaattcg gccggccata acttcgtata atgtatgcta 3000
tacgaagtta tggcaacggt tcatcatctc atggatctgc acatgaacaa acaccagagt 3060
caaacgacgt tgaaattgag gctactgcgc caattgatga caatacagac gatgataaca 3120
aaccgaagtt atctgatgta gaaaaggatt agagatgcta agagatagtg atgatatttc 3180
ataaataatg taattctata tatgttaatt accttttttg cgaggcatat ttatggtgaa 3240
ggataagttt tgaccatcaa agaaggttaa tgtggctgtg gtttcagggt ccataaagct 3300
tttcaattca tctttttttt tttgttcttt tttttgattc cggtttcttt gaaatttttt 3360
tgattcggta atctccgagc agaaggaaga acgaaggaag gagcacagac ttagattggt 3420
atatatacgc atatgtggtg ttgaagaaac atgaaattgc ccagtattct taacccaact 3480
gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga 3540
acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa 3600
gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt 3660
tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt 3720
ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact 3780
cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg 3840
tgtatacaga atagcagaat gggcagacat tacgaatgcg cacggtgtgg tgggcccagg 3900
tattgttagc ggtttgaagc aggcggcgga agaagtaaca aaggaaccta gaggcctttt 3960
gatgttagca gaattgtcat gcaagggctc cctagctact ggagaatata ctaagggtac 4020
tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat 4080
gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga 4140
caagggagac gcattgggtc aacagtatag agccgtggat gatgtggtct ctacaggatc 4200
tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg 4260
tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta 4320
aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta 4380
attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa 4440
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 4500
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 4560
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 4620
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatgcggc 4680
cgcctcgaga tctcccctaa accgtggaat atttcggata tccttttgtt gtttccgggt 4740
gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata 4800
ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac 4860
cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg 4920
tacataacga actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca 4980
ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct 5040
ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat 5100
gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt 5160
ccagagctga tggggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca 5220
cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata 5280
aaaaaagttt gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt 5340
tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag 5400
ctataccaag catacaatca actatctcat atacaatgaa gaacttcata tcactggtga 5460
acaagaaaaa gggtaccctg gatgatagga atagtagcgt tccggaatct tccagtggta 5520
taatacacca acgtggagct ttaaacactg aggattttga agaaggaaag aaagatggtg 5580
cattcgaatt gggtcacctc gaattcacca ccaattcagc ccaattgggt gattcagacg 5640
atgataatga taatgcaatt aagatagcga atgctgccac tgatgaagcc aatgaggcta 5700
atagtgaaga aaaaagcatg accttaaggc aagctttgag aaaatatcca aaggcagccc 5760
tatggtccat cttggtgtct actaccttag tcatggaagg ttatgatact gcgcttttga 5820
gtgcacttta tgcattaccg gttttccaga ggaaattcgg tactatgaat gcggaaggct 5880
cctacgaaat tacctcgcag tggcaaattg gtttgaacat gtgtgtcctt tgtggtgaaa 5940
tgattggttt acagatgacc acttacatgg tcgagttcat gggtaatcgt tacacaatga 6000
ttacggcgct cggcttgttg actgcttata tttttatcct ttactactgc aaaagtttgg 6060
ccatgatcgc tgtagggcaa attctgtctg ctatgccatg gggttgcttc cagagtctgg 6120
ctgttaccta tgcttcggag gtttgccccc tagcgctgag atattacatg accagttact 6180
ccaatatttg ttggttgttt ggtcaaattt tcgcttctgg tatcatgaaa aactcccagg 6240
agaatttggg agactccgat ttaggctaca agttgccatt tgccttacaa tggatctggc 6300
ctgcaccttt gattattggt atcttctttg ctcctgagtc gccttggtgg ctggtgagaa 6360
agaataagat tgcggaggcc aaaaagtcct tgaatagaat cctgagcggc actgctgccg 6420
agagggagat tcaagtggat atcactttaa agcaaattga gatgaccatt gagaaggaga 6480
gacttctggc atctaaatca gggtcgttct tcaactgttt caaaggcgtt gatggaagaa 6540
gaacaaggct tgcgtgtttg acttgggttg ctcaaaacag tagtggtgcc gttttactag 6600
gttactcgac gtatttcttt gaaagggcag ggatggccac tgacaaggcg tttactttct 6660
cgcttatcca gtactgtcta ggtttagcag gcactctttg ttcctgggtg atatctggcc 6720
gtgttggtag atggagtatc ctggcttatg gtcttgcatt tcaaatggtg tgtctattca 6780
tcattggtgg aatggggttt gcatccggaa gcaatgccag taatggtgct ggtggtctac 6840
tgctggcttt atcgttcttt tacaacgctg gtatcggagc tgtcgtttac tgtattgtgg 6900
ctgaaattcc gtctgcagaa ttaaggacca aaactattgt aatggctcgt atttgctata 6960
atttgatggc cgtcatcaat gccattttaa cgccatatat gctgaacgtg agtgactgga 7020
actggggtgc taaaaccggc ctatactggg gtggtttcac tgcagtcact ttggcttggg 7080
ttatcattga tttgcctgag acaactggta gaacatttag cgaaattaat gagcttttca 7140
atcaaggtgt ccctgctaga aaatttgcat ctactgtagt tgatcctttc gggaagggac 7200
agcgtcaaaa tgattcgcaa gtggataacg tcattgacca gtcctcaagc gcaatgcagc 7260
aagagctaaa tgaagctaac gaattctaat taattaaaca ggcccctttt cctttgtcga 7320
tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac 7380
cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat 7440
gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt 7500
gtacgcatgt aacgggcaga cgaattcgat atcaagctta tcgataccgt cgacgcggat 7560
ctcttatgtc tttacgattt atagttttca ttatcaagta tgcctatatt agtatatagc 7620
atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta ctttttgctc 7680
ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt acccattcct 7740
ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc tccaacaaac 7800
agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc tcattttcct 7860
tcgcatgcca gcattgaaat gatcgaagtt caatgatgaa acggtaattc ttctgtcatt 7920
tactcatctc atctcatcaa gttatataat tctatacgga tgtaattttt cacttttcgt 7980
cttgacgtcc accctataat ttcaattatt gaaccctcac aaatgatgca ctgcaatgta 8040
cacaccctca tatagtttct cagggcttga tcagggttcc gtagag 8086
<210> 11
<211> 8685
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、麦卡特酵母和构巢曲霉
<400> 11
ccgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa 60
cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaga 120
cagaggagag cataaaaatg gggttcactt tttggtaaag ctatagcatg cctatcacat 180
ataaatagag tgccagtagc gacttttttc acactcgaaa tactcttact actgctctct 240
tgttgttttt atcacttctt gtttcttctt ggtaaataga atatcaagct acaaaaagca 300
tacctcgagg ccagaaaaag gaagtgtttc cctccttctt gaattgatgt taccctcata 360
aagcacgtgg cctcttatcg agaaagaaat taccgtcgct cgtgatttgt ttgcaaaaag 420
aacaaaactg aaaaaaccca gacacgctcg acttcctgtc ttcctgttga ttgcagcttc 480
caatttcgtc acacaacaag gtcctagcga cggctcacag gttttgtaac aagcaatcga 540
aggttctgga atggcgggaa agggtttagt accacatgct atgatgccca ctgtgatctc 600
cagagcaaag ttcgttcgat cgtactgtta ctctctctct ttcaaacaga attgtccgaa 660
tcgtgtgaca acaacagcct gttctcacac actcttttct tctaaccaag ggggtggttt 720
agtttagtag aacctcgtga aacttacatt tacatatata taaacttgca taaattggtc 780
aatgcaagaa atacatattt ggtcttttct aattcgtagt ttttcaagtt cttagatgct 840
ttctttttct cttttttaca gatcatcaag gaagtaatta tctacttttt acaagtctag 900
aatgactatc tcttctgctc acccagaaac tgaaccaaag tggtggaaag aggcaacttt 960
ttaccaaatc tacccagctt cattcaagga ctccaatgat gatggttggg gtgatatgaa 1020
aggtattgct tccaaattag aatacattaa ggaattaggt gccgatgcta tttggatttc 1080
tccattctat gattctccac aagacgatat gggttatgac atcgctaact atgaaaaggt 1140
ttggccaacc tatggcacta atgaggactg ttttgcatta attgagaaaa cccacaagtt 1200
gggcatgaag ttcattactg atcttgtcat taatcattgt tcatccgaac atgaatggtt 1260
caaggaatcc agatcctcca aaactaatcc aaaaagagat tggtttttct ggagaccacc 1320
taagggttat gatgctgaag gtaagccaat tccaccaaac aattggaagt cttactttgg 1380
tggttccgca tggaccttcg acgaaaagac ccaagagttt tacttgagat tattctgctc 1440
cacccaacca gatttgaact gggaaaatga agattgtaga aaagcaatct acgaatctgc 1500
agttggctat tggttagatc acggtgttga tggtttcaga attgatgttg gttcacttta 1560
ctcaaaggtt gttggtttgc cagatgcacc agttgttgat aaaaactcta catggcaatc 1620
ttctgaccca tacactctta atggtcctag aatccatgaa tttcatcaag agatgaacca 1680
gttcattaga aatagagtta aggatggtag agaaattatg accgttggtg aaatgcaaca 1740
tgcatctgat gaaactaaga gattatacac atcagcctcc cgtcacgaat tgtctgaatt 1800
attcaacttt tcacacacag acgttggcac atccccatta ttccgttata acttggttcc 1860
attcgaattg aaggactgga aaatcgcatt ggcagaattg tttagatata tcaatggtac 1920
tgattgttgg tctaccatct acttggaaaa ccacgaccaa ccaagatcca tcactagatt 1980
cggtgatgac tctcctaaaa accgtgtcat ttctggtaag ttactttctg tcttattatc 2040
cgccttaacc ggtactttgt acgtctatca aggccaggaa ttgggtcaaa ttaactttaa 2100
gaattggcca gtcgaaaagt atgaagatgt cgaaatcaga aacaactaca atgcaattaa 2160
ggaggaacat ggtgaaaatt cagaggaaat gaaaaagttt ttggaagcta ttgctcttat 2220
ttccagagat cacgctagaa ccccaatgca atggtcaaga gaggaaccta acgctggttt 2280
ctctggtcct tccgccaagc cttggtttta cttaaacgac tccttcagag aaggtattaa 2340
cgttgaagat gaaattaagg acccaaattc cgtccttaac ttctggaagg aagcattgaa 2400
gtttagaaag gcccataagg atattaccgt ttatggttat gactttgagt ttatcgattt 2460
ggataacaaa aagttattct cattcactaa aaagtataac aacaagacct tattcgctgc 2520
tttaaacttc tcttctgatg ctactgattt caaaattcct aatgacgatt cctctttcaa 2580
gttggagttt ggtaactacc caaagaagga agttgacgca tcttctcgta cattgaagcc 2640
ttgggaaggt agaatctaca tctccgagta acctgcaggt ttgccagctt actatccttc 2700
ttgaaaatat gcactctata tcttttagtt cttaattgca acacatagat ttgctgtata 2760
acgaatttta tgctattttt taaatttgga gttcagtgat aaaagtgtca cagcgaattt 2820
cctcacatgt agggaccgaa ttgtttacaa gttctctgta ccaccatgga gacatcaaaa 2880
attgaaaatc tatggaaaga tatggacggt agcaacaaga atatagcacg agccggcgac 2940
tagtaacggc cgccagtgtg ctggaattcg gccggccagg ccgcataact tcgtatagca 3000
tacattatac gaagttatcg cctgttaaga tataactgaa aaaagagggg aatttttaga 3060
tactgaaatg atattttaga ataaccagac tatatataag gataaattac aaaaaattaa 3120
ctaatagata agatttaaat ataaaagata tgcaactaga aaagtcttat caatctcctt 3180
atggagtgac gacgttaccc aacaatttac cgacttcttc ggcgatagcc aaagttctct 3240
cttcggacaa tcttctacca ataacttgaa cagcaacagg agcaccgtga taagcctctg 3300
ggtcgtattc ttcttgaacc aaagcatcca attcggaaac agctttaaaa gattcgttct 3360
tcttatcaat attcttatca gcgaaagtga ctgggacgac aacagaggtg aaatccaata 3420
agttaataac ggaggcgtaa ccgtagtatc tgaattgatc gtgtctgaca gcggcggtag 3480
gagtaattgg agcgataata gcgtccaatt ccttaccagc tttttcttca gcttcacgcc 3540
acttttccaa gtattccatt tgatagttcc acttttgtaa atgagtgtcc cacaattcgt 3600
tcatgttaac agccttaata tttgggttca acaagtcctt aatgttaggg atggctggct 3660
caccagaggc agaaatgtct ctcatgacgt cggcagaacc atcagcagca tagatgtggg 3720
aaatcaagtc atgaccgaaa tcatgcttgt atggagtcca tggagtaacg gtgtgaccag 3780
ccttggccaa agcggcaacg gtagtttcga caccacgtaa aattggtggg tgtggcaaga 3840
cgttaccgtc gaaattgtaa taaccaatgt tcaaaccacc attcttaatc ttagaggcaa 3900
tgatgtcaga ttcagattgt ctccatggca ttgggatgac cttagagtcg tacttccaag 3960
gttcttgacc caagacagat ttggtgaaca atctcaagtc ttcgacggag tgagtgatag 4020
gaccaacgac ggagtgaacg gtttcttgac cttccataga gttagccatt ttagcatatg 4080
gcaatctacc gtgagatggt ctcaaaccgt ataaaaagtt gaaagcagct gggactctaa 4140
tggaaccacc aatgtcagta ccgacaccaa taacaccacc tctaatacca acaatagcac 4200
cttcaccacc agaagaacca ccacaggacc aatttttgtt tcttggattg acagttctac 4260
caatgatgtt gttgacggtt tcacagacca tcaaggtttg tgggacagag gtcttaacgt 4320
agaaaacagc accagctttt ctcaacatgg tggttaagac ggaatcacct tcatcgtatt 4380
tgtttaacca ggaaatgtaa cccatggagg tttcgtaacc cttaacacgc aattggtcct 4440
ttaaagagat tggtaaaccg tgtaatggac caactggtct cttatgctta gcgtagtatt 4500
catctaattc tctagcttga gctaaagcag catctgggaa gaattcgtga gcacagttgg 4560
ttaattgttg agcaatagca gctctcttac aaaaagccaa agtgacttca acagaagtca 4620
actcaccagc ggccaacttg gagaccaaat cagcagcaga ggcttcggta atcttcaatt 4680
cagcctcaga caaaataccg gacttctttg ggaaatcaat aacggaatct tcggcaggca 4740
aagtttgaac cttccattcg tcaggaatgg ttttagccaa acgggcacgt ttgtcggcgg 4800
ccaattcttc ccaggattgt ggcattttgt aattaaaact tagattagat tgctatgctt 4860
tctttctaat gagcaagaag taaaaaaagt tgtaatagaa caagaaaaac gaaactgaaa 4920
cttgagaaat tgaagaccat ttattaactt aaatatcaat gggaggtcat cgaaagagaa 4980
aaaaatcaaa aaaaaaattt ttcaagaaaa agaaacgtga taaaaatttt tattgccttt 5040
ttcgacgaag aaaaagaaac gaggcggtct cttttttctt ttccaaacct ttagtacggg 5100
taattaacgc caccctagag gaagaaagag gggaaattta gtatgctgtg cttgggtgtt 5160
ttgaagtggt acggcgatgc gcggagtccg agaaaatctg gaagagtaaa aaaggagtag 5220
aaacattttg aagctatggt gtgtggggga tcacttgtgg gggattgggt gtgatgtaag 5280
gataacttcg tatagcatac attatacgaa gttatgcggc cgcgtctgcc cgttacatgc 5340
gtacacgcgt ttgtacagaa aaaaaagaaa aatttgaaat ataaataacg ttcttaatac 5400
taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa ctccttcctt 5460
ttcggttaga gcggatgtgg gaggagggcg tgaatgtaag cgtgacataa ctaattacat 5520
gatatcgaca aaggaaaagg ggcctgttta attaattaga attcgttagc ttcatttagc 5580
tcttgctgca ttgcgcttga ggactggtca atgacgttat ccacttgcga atcattttga 5640
cgctgtccct tcccgaaagg atcaactaca gtagatgcaa attttctagc agggacacct 5700
tgattgaaaa gctcattaat ttcgctaaat gttctaccag ttgtctcagg caaatcaatg 5760
ataacccaag ccaaagtgac tgcagtgaaa ccaccccagt ataggccggt tttagcaccc 5820
cagttccagt cactcacgtt cagcatatat ggcgttaaaa tggcattgat gacggccatc 5880
aaattatagc aaatacgagc cattacaata gttttggtcc ttaattctgc agacggaatt 5940
tcagccacaa tacagtaaac gacagctccg ataccagcgt tgtaaaagaa cgataaagcc 6000
agcagtagac caccagcacc attactggca ttgcttccgg atgcaaaccc cattccacca 6060
atgatgaata gacacaccat ttgaaatgca agaccataag ccaggatact ccatctacca 6120
acacggccag atatcaccca ggaacaaaga gtgcctgcta aacctagaca gtactggata 6180
agcgagaaag taaacgcctt gtcagtggcc atccctgccc tttcaaagaa atacgtcgag 6240
taacctagta aaacggcacc actactgttt tgagcaaccc aagtcaaaca cgcaagcctt 6300
gttcttcttc catcaacgcc tttgaaacag ttgaagaacg accctgattt agatgccaga 6360
agtctctcct tctcaatggt catctcaatt tgctttaaag tgatatccac ttgaatctcc 6420
ctctcggcag cagtgccgct caggattcta ttcaaggact ttttggcctc cgcaatctta 6480
ttctttctca ccagccacca aggcgactca ggagcaaaga agataccaat aatcaaaggt 6540
gcaggccaga tccattgtaa ggcaaatggc aacttgtagc ctaaatcgga gtctcccaaa 6600
ttctcctggg agtttttcat gataccagaa gcgaaaattt gaccaaacaa ccaacaaata 6660
ttggagtaac tggtcatgta atatctcagc gctagggggc aaacctccga agcataggta 6720
acagccagac tctggaagca accccatggc atagcagaca gaatttgccc tacagcgatc 6780
atggccaaac ttttgcagta gtaaaggata aaaatataag cagtcaacaa gccgagcgcc 6840
gtaatcattg tgtaacgatt acccatgaac tcgaccatgt aagtggtcat ctgtaaacca 6900
atcatttcac cacaaaggac acacatgttc aaaccaattt gccactgcga ggtaatttcg 6960
taggagcctt ccgcattcat agtaccgaat ttcctctgga aaaccggtaa tgcataaagt 7020
gcactcaaaa gcgcagtatc ataaccttcc atgactaagg tagtagacac caagatggac 7080
catagggctg cctttggata ttttctcaaa gcttgcctta aggtcatgct tttttcttca 7140
ctattagcct cattggcttc atcagtggca gcattcgcta tcttaattgc attatcatta 7200
tcatcgtctg aatcacccaa ttgggctgaa ttggtggtga attcgaggtg acccaattcg 7260
aatgcaccat ctttctttcc ttcttcaaaa tcctcagtgt ttaaagctcc acgttggtgt 7320
attataccac tggaagattc cggaacgcta ctattcctat catccagggt accctttttc 7380
ttgttcacca gtgatatgaa gttcttcatt gtatatgaga tagttgattg tatgcttggt 7440
atagcttgaa atattgtgca gaaaaagaaa caaggaagaa agggaacgag aacaatgacg 7500
aggaaacaaa agattaataa ttgcaggtct atttatactt gatagcaaga cagcaaactt 7560
tttttatttc aaattcaagt aactggaagg aaggccgtat accgttgctc attagagagt 7620
agtgtgcgtg aatgaaggaa ggaaaaagtt tcgtgtgctt cgagataccc cccatcagct 7680
ctggaacaac gacatctgtt ggtgctgtct ttgtcgttaa ttttttcctt tagtgtcttc 7740
catcattttt ttgtcattgc ggatatggtg agacaacaac gggggagaga gaaaagaaaa 7800
aaaaagaaaa gaagttgcat gcgcctatta ttacttcaat agatggcaaa tggaaaaagg 7860
gtagtgaaac ttcgatatga tgatggctat caagtctagg gctacagtat tagttcgtta 7920
tgtaccacca tcaatgaggc agtgtaattg gtgtagtctt gtttagccca ttatgtcttg 7980
tctggtatct gttctattgt atatctcccc tccgccacct acatgttagg gagaccaacg 8040
aaggtattat aggaatcccg atgtatgggt ttggttgcca gaaaagagga agtccatatt 8100
gtacacccgg aaacaacaaa aggatatccg aaatattcca cggtttaggt cgacgcggat 8160
ctcttatgtc tttacgattt atagttttca ttatcaagta tgcctatatt agtgtatagc 8220
atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta ctttttgctc 8280
ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt acccattcct 8340
ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc tccaacaaac 8400
agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc tcattttcct 8460
tcgcatgcca gcattgaaat gatcgaagtt caatgatgaa acggtaattc ttctgtcatt 8520
tactcatctc atctcatcaa gttatataat tctatacgga tgtaattttt cacttttcgt 8580
cttgacgtca ccctataatt tcaattgttg aaccctcaca aatgatgcac tgcaatgtac 8640
acaccctcat atagtttctc agggcttgat cagggttccg tagag 8685
<210> 12
<211> 1632
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 12
ggcaacggtt catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt 60
gaaattgagg ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta 120
tctgatgtag aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt 180
aattctatat atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt 240
gaccatcaaa gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat 300
cttttttttt tttgttcttt tttttgattc cggtttcttt gaaatttttt tgattcggta 360
atctccgagc agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc 420
atatgtggtg ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca 480
aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct 540
actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac 600
ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta 660
ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag 720
ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac 780
agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga 840
atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc 900
ggtttgaagc aggcggcgga agaagtaaca aaggaaccta gaggcctttt gatgttagca 960
gaattgtcat gcaagggctc cctagctact ggagaatata ctaagggtac tgttgacatt 1020
gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga 1080
gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac 1140
gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt 1200
attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac 1260
agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta 1320
ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag 1380
ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga 1440
aagaaaaagc ttcatggcct ttataaaaag gaaccatcca atacctcgcc agaaccaagt 1500
aacagtattt tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca 1560
ttgaactcca aagaacaaca agagttccaa aaagtagtgg aacaaaagca aatgaaggat 1620
ttcatgcgtt tg 1632
<210> 13
<211> 4701
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 13
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgctaaaat acaaaccttt 1560
actaaaaatc tcgaagaact gtgaggctgc tatcctcaga gcgtctaaga ctagattgaa 1620
cacaatccgc gcgtacggtt ctaccgttcc aaaatccaag tcgttcgaac aagactcaag 1680
aaaacgcaca cagtcatgga ctgccttgag agtcggtgca attctagccg ctactagttc 1740
cgtggcgtat ctaaactggc ataatggcca aatagacaac gagccgaaac tggatatgaa 1800
taaacaaaag atttcgcccg ctgaagttgc caagcataac aagcccgatg attgttgggt 1860
tgtgatcaat ggttacgtat acgacttaac gcgattccta ccaaatcatc caggtgggca 1920
ggatgttatc aagtttaacg ccgggaaaga tgtcactgct atttttgaac cactacatgc 1980
tcctaatgtc atcgataagt atatagctcc cgagaaaaaa ttgggtcccc ttcaaggatc 2040
catgcctcct gaacttgtct gtcctcctta tgctcctggt gaaactaagg aagatatcgc 2100
tagaaaagaa caactaaaat cgctgctacc tcctctagat aatattatta acctttacga 2160
ctttgaatac ttggcctctc aaactttgac taaacaagcg tgggcctact attcctccgg 2220
tgctaacgac gaagttactc acagagaaaa ccataatgct tatcatagga tttttttcaa 2280
accaaagatc cttgtagatg tacgcaaagt agacatttca actgacatgt tgggttctca 2340
tgtggatgtt cccttctacg tgtctgctac agctttgtgt aaactgggaa accccttaga 2400
aggtgaaaaa gatgtcgcca gaggttgtgg ccaaggtgtg acaaaagtcc cacaaatgat 2460
atctactttg gcttcatgtt cccctgagga aattattgaa gcagcaccct ctgataaaca 2520
aattcaatgg taccaactat atgttaactc tgatagaaag atcactgatg atttggttaa 2580
aaatgtagaa aagctgggtg taaaggcatt atttgtcact gtggatgctc caagtttagg 2640
tcaaagagaa aaagatatga agctgaaatt ttccaataca aaggctggtc caaaagcgat 2700
gaagaaaact aatgtagaag aatctcaagg tgcttcgaga gcgttatcaa agtttattga 2760
cccctctttg acttggaaag atatagaaga gttgaagaaa aagacaaaac tacctattgt 2820
tatcaaaggt gttcaacgta ccgaagatgt tatcaaagca gcagaaatcg gtgtaagtgg 2880
ggtggttcta tccaatcatg gtggtagaca attagatttt tcaagggctc ccattgaagt 2940
cctggctgaa accatgccaa tcctggaaca acgtaacttg aaggataagt tggaagtttt 3000
cgtggacggt ggtgttcgtc gtggtacaga tgtcttgaaa gcgttatgtc taggtgctaa 3060
aggtgttggt ttgggtagac cattcttgta tgcgaactca tgctatggtc gtaatggtgt 3120
tgaaaaagcc attgaaattt taagagatga aattgaaatg tctatgagac tattaggtgt 3180
tactagcatt gcggaattga agcctgatct tttagatcta tcaacactaa aggcaagaac 3240
agttggagta ccaaacgacg tgctgtataa tgaagtttat gagggaccta ctttaacaga 3300
atttgaggat gcatgattaa ttaaacaggc cccttttcct ttgtcgatat catgtaatta 3360
gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga 3420
gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 3480
acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac 3540
gggcagacgg ccggccataa cttcgtataa tgtatgctat acgaagttat ggcaacggtt 3600
catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt gaaattgagg 3660
ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta tctgatgtag 3720
aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt aattctatat 3780
atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa 3840
gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat cttttttttt 3900
tttgttcttt tttttgattc cggtttcttt gaaatttttt tgattcggta atctccgagc 3960
agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtggtg 4020
ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca 4080
ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta 4140
gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt 4200
cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa 4260
tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta 4320
agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg 4380
ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat 4440
gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc 4500
aggcggcgga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat 4560
gcaagggctc cctagctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg 4620
acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt 4680
acgattggtt gattatgaca c 4701
<210> 14
<211> 3126
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 14
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agtaatttac cagcttacta tccttcttga 1740
aaatatgcac tctatatctt ttagttctta attgcaacac atagatttgc tgtataacga 1800
attttatgct atttttttaa tttggagttc ggtgatgaaa gtgtcacagc gaatttcctc 1860
acatgtaggg accgaattgt ttacaagttc tctgtaccac catggagaca tcaaagattg 1920
aaaatctatg gaaagatatg gacggtagca acaagaatat agcacgagcc gcggagttca 1980
tttcgttact tttgatatcg ctcacaacta ttgcgaagcg cttcagtgaa aaaatcataa 2040
ggaaaagttg taaatattat tggtagtatt cgtttggtaa agtagagggg gtaatttttc 2100
ccctttattt tgttcataca ttcttaaatt gctttgcctc tccttttgga aagctatact 2160
tcggagcact gttgagcgaa ggctcaggcc ggcatatgac gttttattac ctttgatcac 2220
atttccacgc catttcgcat tctcaccctc ataagtcata caccgaaaag aaagtttaag 2280
ggatcaatga gcttactata atctcagtat atttattttt atcgatgatt caccacaaca 2340
atcttgctcc cgaaaagaaa gcagacggag tagaagcatt tgaaactcct tcagaccttc 2400
aagtatatat atatatatat atatatgtat atgtgtacat tttcacgcta atactaatgt 2460
ataattagaa gataattttt actcattttt cgttatcttc acgtcacccg aacctagaac 2520
caaatgtcat tttcacgata tgtaaatagt gaaataggca aaaacgccaa aaagtagtaa 2580
gcgcaacata cactaaacca ttaaagaata tctcgaccag aatctaacag atatacatgt 2640
tccgataatg tctgagttag gtgagtattc taaattagaa aacaaagagc ttagaacgga 2700
gtttgaattg acaaattttc cttttccagg cacaactgat aacgactccg atgacggaag 2760
ccaagggcag aactctttga atatcattac tcctgacatg gatgatactc tggttaatga 2820
tgtacttcga gaaaacgata aaaagtctag tatgagaatg gcttttatga atctagcaaa 2880
ctctattctt ggtgccggaa taattactca gccgttcgcg atcaaaaatg ctggtatatt 2940
aggcgggcta ttatcatacg tagccctcgg atttatagtt gattggacgt taagacttat 3000
tgtcattaac ttgactcttg ctggcaagag aacataccag ggtacggtcg aacatgtaat 3060
gggtaaaaaa gggaaattgc tgattctatt tacaaacggg ttatttgcat ttggtggatg 3120
tattgg 3126
<210> 15
<211> 591
<212> PRT
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 15
Met Leu Lys Tyr Lys Pro Leu Leu Lys Ile Ser Lys Asn Cys Glu Ala
1 5 10 15
Ala Ile Leu Arg Ala Ser Lys Thr Arg Leu Asn Thr Ile Arg Ala Tyr
20 25 30
Gly Ser Thr Val Pro Lys Ser Lys Ser Phe Glu Gln Asp Ser Arg Lys
35 40 45
Arg Thr Gln Ser Trp Thr Ala Leu Arg Val Gly Ala Ile Leu Ala Ala
50 55 60
Thr Ser Ser Val Ala Tyr Leu Asn Trp His Asn Gly Gln Ile Asp Asn
65 70 75 80
Glu Pro Lys Leu Asp Met Asn Lys Gln Lys Ile Ser Pro Ala Glu Val
85 90 95
Ala Lys His Asn Lys Pro Asp Asp Cys Trp Val Val Ile Asn Gly Tyr
100 105 110
Val Tyr Asp Leu Thr Arg Phe Leu Pro Asn His Pro Gly Gly Gln Asp
115 120 125
Val Ile Lys Phe Asn Ala Gly Lys Asp Val Thr Ala Ile Phe Glu Pro
130 135 140
Leu His Ala Pro Asn Val Ile Asp Lys Tyr Ile Ala Pro Glu Lys Lys
145 150 155 160
Leu Gly Pro Leu Gln Gly Ser Met Pro Pro Glu Leu Val Cys Pro Pro
165 170 175
Tyr Ala Pro Gly Glu Thr Lys Glu Asp Ile Ala Arg Lys Glu Gln Leu
180 185 190
Lys Ser Leu Leu Pro Pro Leu Asp Asn Ile Ile Asn Leu Tyr Asp Phe
195 200 205
Glu Tyr Leu Ala Ser Gln Thr Leu Thr Lys Gln Ala Trp Ala Tyr Tyr
210 215 220
Ser Ser Gly Ala Asn Asp Glu Val Thr His Arg Glu Asn His Asn Ala
225 230 235 240
Tyr His Arg Ile Phe Phe Lys Pro Lys Ile Leu Val Asp Val Arg Lys
245 250 255
Val Asp Ile Ser Thr Asp Met Leu Gly Ser His Val Asp Val Pro Phe
260 265 270
Tyr Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Leu Glu Gly
275 280 285
Glu Lys Asp Val Ala Arg Gly Cys Gly Gln Gly Val Thr Lys Val Pro
290 295 300
Gln Met Ile Ser Thr Leu Ala Ser Cys Ser Pro Glu Glu Ile Ile Glu
305 310 315 320
Ala Ala Pro Ser Asp Lys Gln Ile Gln Trp Tyr Gln Leu Tyr Val Asn
325 330 335
Ser Asp Arg Lys Ile Thr Asp Asp Leu Val Lys Asn Val Glu Lys Leu
340 345 350
Gly Val Lys Ala Leu Phe Val Thr Val Asp Ala Pro Ser Leu Gly Gln
355 360 365
Arg Glu Lys Asp Met Lys Leu Lys Phe Ser Asn Thr Lys Ala Gly Pro
370 375 380
Lys Ala Met Lys Lys Thr Asn Val Glu Glu Ser Gln Gly Ala Ser Arg
385 390 395 400
Ala Leu Ser Lys Phe Ile Asp Pro Ser Leu Thr Trp Lys Asp Ile Glu
405 410 415
Glu Leu Lys Lys Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val Gln
420 425 430
Arg Thr Glu Asp Val Ile Lys Ala Ala Glu Ile Gly Val Ser Gly Val
435 440 445
Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala Pro
450 455 460
Ile Glu Val Leu Ala Glu Thr Met Pro Ile Leu Glu Gln Arg Asn Leu
465 470 475 480
Lys Asp Lys Leu Glu Val Phe Val Asp Gly Gly Val Arg Arg Gly Thr
485 490 495
Asp Val Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly
500 505 510
Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly Arg Asn Gly Val Glu
515 520 525
Lys Ala Ile Glu Ile Leu Arg Asp Glu Ile Glu Met Ser Met Arg Leu
530 535 540
Leu Gly Val Thr Ser Ile Ala Glu Leu Lys Pro Asp Leu Leu Asp Leu
545 550 555 560
Ser Thr Leu Lys Ala Arg Thr Val Gly Val Pro Asn Asp Val Leu Tyr
565 570 575
Asn Glu Val Tyr Glu Gly Pro Thr Leu Thr Glu Phe Glu Asp Ala
580 585 590
<210> 16
<211> 4662
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和东方伊萨酵母。
<400> 16
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgttactca gatcactaaa 1560
ctcttctgct cgttgtgtca aacaaacaac cagaacaaag gttaggtatc tcagccacgt 1620
cagtggtgca agcatggcga aacctacatt gaagaacaac tcgagagaat ccaacaaatc 1680
cagaaactat ctaattgctg ctgtgacagc attggctgta tcaacctcaa ttggagttgc 1740
cgtacatgtg aaggacccct tgtataacga tgctaccggc agtgattctc cgagaagtat 1800
atctgttgac gagtttgtca agcataattc acaaaacgac tgttggattg caatcaatgg 1860
caaggtttat gatttcactg attttattcc aaaccatcca ggtggggtac ctccattagt 1920
taatcatgct ggttatgatg gtactaaact ttatgagaaa ttgcatccaa aaggtacaat 1980
tgagaaattc ttgccaaagg ataagtttct gggtgtgtta gatggtgaag cgccaaaatt 2040
ggaagcagac tatttggtgg acgatgatga acaagagaga ctggattatt tgaacaactt 2100
acctcctttg tcatctattc agaatgttta tgatttcgaa tacttggcca agaagatttt 2160
acctaaagat gcctgggcat attattcttg tggtgccgat gatgaaatca caatgagaga 2220
aaaccattat gcttatcaaa gagtttattt cagaccaaga atttgtgttg atgtcaagga 2280
agttgatact tcttatgaaa tgttaggcac taaaacctct gttccttttt atgtatctgc 2340
caccgctttg gctaaattag gccatcctga tggtgaatgc tcaattgcta gaggcgctgg 2400
taaggaaggt gtcgttcaaa tgatttcgac cctttcctca atgtcattag atgaaattgc 2460
cgctgctaga atcccaggtg caacccaatg gttccaatta tacattaatg aggatagaaa 2520
tgtcgctaaa ggtctggtca aacatgcaga agacttgggt atgaaggcta tctttataac 2580
tgttgatgct ccttctctag gtaacagaga aaaggataaa agattaaagt ttgttaatga 2640
caccgatgtc gatttgggtg attccgcaga tcgaaacagt ggtgcttcaa aggcactatc 2700
ttcgttcatt gatgcttctg tctcttggaa tgacgtcaaa gcggtcaagt cgtggactaa 2760
attgcctgtc ttagttaaag gtgttcaaac agttgaagac gttattgaag cttacgatgc 2820
tggttgtcaa ggtgttgttt tgtcaaacca cggtggtagg caactagata ctgctcctcc 2880
tccaatcgaa ttattagctg aaactgttcc aactttgaag agattgggta aattaagacc 2940
agattttgaa attttaattg acggtggtgt caaaagaggt accgatattt tgaaagcagt 3000
cgcaatcggt ggccaagatg tcagagtttc agttggtatg ggtagacctt tcttatatgc 3060
caactcttgc tatggtgaag caggtgttag aaaattaatt caaaatctaa aggatgaatt 3120
agaaatggat atgagattgt tgggtgtcac taaaatggac cagctatctt cgaaacatgt 3180
cgatactaaa cgtttgattg gtagagatgc gatcaactat ttgtatgata atgtatacag 3240
cccaatcgaa accgttaaat tcaacaatga agattgatta attaaacagg ccccttttcc 3300
tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc 3360
gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt 3420
atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac 3480
aaacgcgtgt acgcatgtaa cgggcagacg gccggccata acttcgtata atgtatgcta 3540
tacgaagtta tggcaacggt tcatcatctc atggatctgc acatgaacaa acaccagagt 3600
caaacgacgt tgaaattgag gctactgcgc caattgatga caatacagac gatgataaca 3660
aaccgaagtt atctgatgta gaaaaggatt agagatgcta agagatagtg atgatatttc 3720
ataaataatg taattctata tatgttaatt accttttttg cgaggcatat ttatggtgaa 3780
ggataagttt tgaccatcaa agaaggttaa tgtggctgtg gtttcagggt ccataaagct 3840
tttcaattca tctttttttt ttttgttctt ttttttgatt ccggtttctt tgaaattttt 3900
ttgattcggt aatctccgag cagaaggaag aacgaaggaa ggagcacaga cttagattgg 3960
tatatatacg catatgtggt gttgaagaaa catgaaattg cccagtattc ttaacccaac 4020
tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg 4080
aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa 4140
agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag 4200
ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt 4260
tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac 4320
tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg 4380
gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag 4440
gtattgttag cggtttgaag caggcggcgg aagaagtaac aaaggaacct agaggccttt 4500
tgatgttagc agaattgtca tgcaagggct ccctagctac tggagaatat actaagggta 4560
ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca 4620
tgggtggaag agatgaaggt tacgattggt tgattatgac ac 4662
<210> 17
<211> 578
<212> PRT
<213> 东方伊萨酵母(Issatchenkia orientalis)
<400> 17
Met Leu Leu Arg Ser Leu Asn Ser Ser Ala Arg Cys Val Lys Gln Thr
1 5 10 15
Thr Arg Thr Lys Val Arg Tyr Leu Ser His Val Ser Gly Ala Ser Met
20 25 30
Ala Lys Pro Thr Leu Lys Asn Asn Ser Arg Glu Ser Asn Lys Ser Arg
35 40 45
Asn Tyr Leu Ile Ala Ala Val Thr Ala Leu Ala Val Ser Thr Ser Ile
50 55 60
Gly Val Ala Val His Val Lys Asp Pro Leu Tyr Asn Asp Ala Thr Gly
65 70 75 80
Ser Asp Ser Pro Arg Ser Ile Ser Val Asp Glu Phe Val Lys His Asn
85 90 95
Ser Gln Asn Asp Cys Trp Ile Ala Ile Asn Gly Lys Val Tyr Asp Phe
100 105 110
Thr Asp Phe Ile Pro Asn His Pro Gly Gly Val Pro Pro Leu Val Asn
115 120 125
His Ala Gly Tyr Asp Gly Thr Lys Leu Tyr Glu Lys Leu His Pro Lys
130 135 140
Gly Thr Ile Glu Lys Phe Leu Pro Lys Asp Lys Phe Leu Gly Val Leu
145 150 155 160
Asp Gly Glu Ala Pro Lys Leu Glu Ala Asp Tyr Leu Val Asp Asp Asp
165 170 175
Glu Gln Glu Arg Leu Asp Tyr Leu Asn Asn Leu Pro Pro Leu Ser Ser
180 185 190
Ile Gln Asn Val Tyr Asp Phe Glu Tyr Leu Ala Lys Lys Ile Leu Pro
195 200 205
Lys Asp Ala Trp Ala Tyr Tyr Ser Cys Gly Ala Asp Asp Glu Ile Thr
210 215 220
Met Arg Glu Asn His Tyr Ala Tyr Gln Arg Val Tyr Phe Arg Pro Arg
225 230 235 240
Ile Cys Val Asp Val Lys Glu Val Asp Thr Ser Tyr Glu Met Leu Gly
245 250 255
Thr Lys Thr Ser Val Pro Phe Tyr Val Ser Ala Thr Ala Leu Ala Lys
260 265 270
Leu Gly His Pro Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys
275 280 285
Glu Gly Val Val Gln Met Ile Ser Thr Leu Ser Ser Met Ser Leu Asp
290 295 300
Glu Ile Ala Ala Ala Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu
305 310 315 320
Tyr Ile Asn Glu Asp Arg Asn Val Ala Lys Gly Leu Val Lys His Ala
325 330 335
Glu Asp Leu Gly Met Lys Ala Ile Phe Ile Thr Val Asp Ala Pro Ser
340 345 350
Leu Gly Asn Arg Glu Lys Asp Lys Arg Leu Lys Phe Val Asn Asp Thr
355 360 365
Asp Val Asp Leu Gly Asp Ser Ala Asp Arg Asn Ser Gly Ala Ser Lys
370 375 380
Ala Leu Ser Ser Phe Ile Asp Ala Ser Val Ser Trp Asn Asp Val Lys
385 390 395 400
Ala Val Lys Ser Trp Thr Lys Leu Pro Val Leu Val Lys Gly Val Gln
405 410 415
Thr Val Glu Asp Val Ile Glu Ala Tyr Asp Ala Gly Cys Gln Gly Val
420 425 430
Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ala Pro Pro Pro
435 440 445
Ile Glu Leu Leu Ala Glu Thr Val Pro Thr Leu Lys Arg Leu Gly Lys
450 455 460
Leu Arg Pro Asp Phe Glu Ile Leu Ile Asp Gly Gly Val Lys Arg Gly
465 470 475 480
Thr Asp Ile Leu Lys Ala Val Ala Ile Gly Gly Gln Asp Val Arg Val
485 490 495
Ser Val Gly Met Gly Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly
500 505 510
Glu Ala Gly Val Arg Lys Leu Ile Gln Asn Leu Lys Asp Glu Leu Glu
515 520 525
Met Asp Met Arg Leu Leu Gly Val Thr Lys Met Asp Gln Leu Ser Ser
530 535 540
Lys His Val Asp Thr Lys Arg Leu Ile Gly Arg Asp Ala Ile Asn Tyr
545 550 555 560
Leu Tyr Asp Asn Val Tyr Ser Pro Ile Glu Thr Val Lys Phe Asn Asn
565 570 575
Glu Asp
<210> 18
<211> 4419
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和黑曲霉。
<400> 18
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgtctaaag gaaaactaac 1560
tggcgcagct gtcgcggaac acaactctaa ggattcatgt tgggttattg ttcatggtaa 1620
ggcatacgat gtgacagaat tcttgccaga acacccagga ggccaaaaga ttatactgaa 1680
atacgctggg aaagatgcaa cagaagagtt cgatcctatt cacccaccag atacattgga 1740
taagtacttg gactcatcaa agcatttagg tgaggtggat atgactactg tcgaacaaga 1800
ggaaaagacc gcagatccag aagaggaggc aagacaggaa cgtattaagt gcatgccaag 1860
tcttgcagcg tgttacaatt tgatggattt tgaaacagta gcacgttcag ttatgaaaaa 1920
gaccgcgtgg gcttattaca gttctggtgc tgacgatgaa atcacaatga gagagaacca 1980
ctctgctttt cataagatct ggttcagacc tagagttttg gtcgacgtag aacatgtgga 2040
tttctctacc actatgttag gtacaaaggt ttcagtccca ttctatgtaa ctgcaacagc 2100
cttaggaaag ttgggtaacc cagaaggtga ggttgttctg accagagcag cacacacaca 2160
tgatgttatt caaatgatac caacattagc ctcatgcagc ttcgatgaaa tcgttgatgc 2220
cagacagggg gatcaagtcc agtggcttca actctacgtt aacaaggata gaaatatcac 2280
taagagaatc gttcaacatg ctgaggcaag aggatgcaaa ggtttattca tcacagttga 2340
tgcccctcaa ttaggtagaa gagaaaaaga catgagatca aagttttccg acgtaggttc 2400
taacgtacaa gcttctggtg gcagctccgt tgatagatct caaggtgcag ccagagccat 2460
ctctagtttc atcgatccag ccctgtcatg gaaagatatt ccttggtttc aatccatcac 2520
taagatgcct atactattga aaggtgtgca atgtgttgag gacgtgctta gagcggtaga 2580
aatgggtgtc caaggtgttg tgctatccaa ccatgggggt agacagttag aattcgcacg 2640
ttctgctatt gaagtgcttg cagaagtcat gcctatattg agagaaagag gctgggagaa 2700
taagatagag atttacattg acggcgggat tcgtagagcc acagatatgc tcaaagcact 2760
ctgtctagga gctaaaggtg ttggaatcgg cagaccattt ttgtacgcta tgtcagctta 2820
cggccaacca ggtgtcgaaa gagctatgca gttattgaag gacgaaatgg aaatgaatat 2880
gagacttatt ggggctacca agattgagga attgaaccca tctctaatcg acgttagagg 2940
attagtcggt ggtcattctg ctccagttcc aagtgataca cttactactg gagcttacga 3000
ccctttgcaa gcccctagat tctccgaaaa gtagttaatt aaacaggccc cttttccttt 3060
gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc cacatccgct 3120
ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 3180
gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 3240
cgcgtgtacg catgtaacgg gcagacggcc ggccataact tcgtataatg tatgctatac 3300
gaagttatgg caacggttca tcatctcatg gatctgcaca tgaacaaaca ccagagtcaa 3360
acgacgttga aattgaggct actgcgccaa ttgatgacaa tacagacgat gataacaaac 3420
cgaagttatc tgatgtagaa aaggattaga gatgctaaga gatagtgatg atatttcata 3480
aataatgtaa ttctatatat gttaattacc ttttttgcga ggcatattta tggtgaagga 3540
taagttttga ccatcaaaga aggttaatgt ggctgtggtt tcagggtcca taaagctttt 3600
caattcatct tttttttttt tgttcttttt tttgattccg gtttctttga aatttttttg 3660
attcggtaat ctccgagcag aaggaagaac gaaggaagga gcacagactt agattggtat 3720
atatacgcat atgtggtgtt gaagaaacat gaaattgccc agtattctta acccaactgc 3780
acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac 3840
gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc 3900
aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg 3960
aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt 4020
ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct 4080
tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg 4140
tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta 4200
ttgttagcgg tttgaagcag gcggcggaag aagtaacaaa ggaacctaga ggccttttga 4260
tgttagcaga attgtcatgc aagggctccc tagctactgg agaatatact aagggtactg 4320
ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg 4380
gtggaagaga tgaaggttac gattggttga ttatgacac 4419
<210> 19
<211> 497
<212> PRT
<213> 黑曲霉(Aspergillus niger)
<400> 19
Met Ser Lys Gly Lys Leu Thr Gly Ala Ala Val Ala Glu His Asn Ser
1 5 10 15
Lys Asp Ser Cys Trp Val Ile Val His Gly Lys Ala Tyr Asp Val Thr
20 25 30
Glu Phe Leu Pro Glu His Pro Gly Gly Gln Lys Ile Ile Leu Lys Tyr
35 40 45
Ala Gly Lys Asp Ala Thr Glu Glu Phe Asp Pro Ile His Pro Pro Asp
50 55 60
Thr Leu Asp Lys Tyr Leu Asp Ser Ser Lys His Leu Gly Glu Val Asp
65 70 75 80
Met Thr Thr Val Glu Gln Glu Glu Lys Thr Ala Asp Pro Glu Glu Glu
85 90 95
Ala Arg Gln Glu Arg Ile Lys Cys Met Pro Ser Leu Ala Ala Cys Tyr
100 105 110
Asn Leu Met Asp Phe Glu Thr Val Ala Arg Ser Val Met Lys Lys Thr
115 120 125
Ala Trp Ala Tyr Tyr Ser Ser Gly Ala Asp Asp Glu Ile Thr Met Arg
130 135 140
Glu Asn His Ser Ala Phe His Lys Ile Trp Phe Arg Pro Arg Val Leu
145 150 155 160
Val Asp Val Glu His Val Asp Phe Ser Thr Thr Met Leu Gly Thr Lys
165 170 175
Val Ser Val Pro Phe Tyr Val Thr Ala Thr Ala Leu Gly Lys Leu Gly
180 185 190
Asn Pro Glu Gly Glu Val Val Leu Thr Arg Ala Ala His Thr His Asp
195 200 205
Val Ile Gln Met Ile Pro Thr Leu Ala Ser Cys Ser Phe Asp Glu Ile
210 215 220
Val Asp Ala Arg Gln Gly Asp Gln Val Gln Trp Leu Gln Leu Tyr Val
225 230 235 240
Asn Lys Asp Arg Asn Ile Thr Lys Arg Ile Val Gln His Ala Glu Ala
245 250 255
Arg Gly Cys Lys Gly Leu Phe Ile Thr Val Asp Ala Pro Gln Leu Gly
260 265 270
Arg Arg Glu Lys Asp Met Arg Ser Lys Phe Ser Asp Val Gly Ser Asn
275 280 285
Val Gln Ala Ser Gly Gly Ser Ser Val Asp Arg Ser Gln Gly Ala Ala
290 295 300
Arg Ala Ile Ser Ser Phe Ile Asp Pro Ala Leu Ser Trp Lys Asp Ile
305 310 315 320
Pro Trp Phe Gln Ser Ile Thr Lys Met Pro Ile Leu Leu Lys Gly Val
325 330 335
Gln Cys Val Glu Asp Val Leu Arg Ala Val Glu Met Gly Val Gln Gly
340 345 350
Val Val Leu Ser Asn His Gly Gly Arg Gln Leu Glu Phe Ala Arg Ser
355 360 365
Ala Ile Glu Val Leu Ala Glu Val Met Pro Ile Leu Arg Glu Arg Gly
370 375 380
Trp Glu Asn Lys Ile Glu Ile Tyr Ile Asp Gly Gly Ile Arg Arg Ala
385 390 395 400
Thr Asp Met Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Ile
405 410 415
Gly Arg Pro Phe Leu Tyr Ala Met Ser Ala Tyr Gly Gln Pro Gly Val
420 425 430
Glu Arg Ala Met Gln Leu Leu Lys Asp Glu Met Glu Met Asn Met Arg
435 440 445
Leu Ile Gly Ala Thr Lys Ile Glu Glu Leu Asn Pro Ser Leu Ile Asp
450 455 460
Val Arg Gly Leu Val Gly Gly His Ser Ala Pro Val Pro Ser Asp Thr
465 470 475 480
Leu Thr Thr Gly Ala Tyr Asp Pro Leu Gln Ala Pro Arg Phe Ser Glu
485 490 495
Lys
<210> 20
<211> 4398
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和解脂耶氏酵母。
<400> 20
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atggtttcag ttgaagaggt 1560
tgctaaacat aacagtaagg attcatgttg ggttatactt catggtaagg catacgactt 1620
gacagaattc ttaccagaac atcctggtgg gcaagcgatt attctcaagt acgctggtaa 1680
ggatgcaaca aaggctttcg atccaataca tcctagagat gttgtcgata agttcttgga 1740
caaagacaaa caccttggtg aggtaactgg tgaaattgca gctgaggaag aggaagaggt 1800
cacaccagag gagaaggcca gactggaaag atatgaaaac agaccacctt tgtcccaaat 1860
ctttaactct tttgattttg aatacgtggc aagacacacc atgtccccaa acgcctgggc 1920
ctattactct tctgggagcg atgacgagat cactgtcaga gaaaatcata gagctttcca 1980
caaaatctgg tttcgtccaa gagttctagt ggatgtcaaa aatgttgata tctcaacaac 2040
aatgcttggg actaaatcat cagttccatt ctacattaca gcgacagcat tgggtaaatt 2100
gggacaccca gaaggtgaag tggttcttac tagaggagct gataagatgg acgtgatcca 2160
aatgatacct accttagcat catgttcttt cgatgaaatt gtcgatgctg ccaccgataa 2220
gcagactcaa tggatgcaac tgtatgtaaa catggataga gaagttacta aaaagattgt 2280
gcaacatgca gaaaagagag gcgttaaggg cctgtttatc acagttgacg caccacaatt 2340
aggcagaaga gaaaaggaca tgagaacaaa gttcggtgat ccaggcgctc aagtacagca 2400
atccgatgat tctgtggaca gatctcaggg agctgctaga gctatctcaa gcttcattga 2460
tccttcctta tcatggaagg acattccttg gttccagagt atcacaaaga tgcctatcat 2520
cttaaaaggt gtgcaatgtg ccgaagatgc gctaaaggct gtagagtaca aagtagacgg 2580
tattttgtta tctaaccatg gtggacgtca gctagagttt gccagaccat ctattgaggt 2640
cctcgttgaa gtaatggcag ctttaagagc caagggttgg caagattaca tagaagttta 2700
catagacgga ggcatcagac gtgcaactga tgtcattaag gctctatgcc taggtgcaaa 2760
aggagtcggg attggcagac catttctcta cgcgatgtct acctacggag aagatggtgt 2820
ttgccacttg attcaattgt tgaaagatga aatggaaatg aatatgagac ttatcggtgc 2880
cacaaagata gaggatttga atccaagtat ggttgatttg aagtctattt tcactcattc 2940
tgcagacaca gcaagagata tcttaggcga aaacgtttac caacatatgg aaatgccatt 3000
attcaagggt tagttaatta aacaggcccc ttttcctttg tcgatatcat gtaattagtt 3060
atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa ggaaggagtt 3120
agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg 3180
ttatttatat ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacggg 3240
cagacggccg gccataactt cgtataatgt atgctatacg aagttatggc aacggttcat 3300
catctcatgg atctgcacat gaacaaacac cagagtcaaa cgacgttgaa attgaggcta 3360
ctgcgccaat tgatgacaat acagacgatg ataacaaacc gaagttatct gatgtagaaa 3420
aggattagag atgctaagag atagtgatga tatttcataa ataatgtaat tctatatatg 3480
ttaattacct tttttgcgag gcatatttat ggtgaaggat aagttttgac catcaaagaa 3540
ggttaatgtg gctgtggttt cagggtccat aaagcttttc aattcatctt tttttttttt 3600
gttctttttt ttgattccgg tttctttgaa atttttttga ttcggtaatc tccgagcaga 3660
aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtggtgttg 3720
aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga 3780
aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc 3840
ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat 3900
tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt 3960
gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc 4020
cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg 4080
acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg 4140
cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg 4200
cggcggaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca 4260
agggctccct agctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca 4320
aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg 4380
attggttgat tatgacac 4398
<210> 21
<211> 490
<212> PRT
<213> 解脂耶氏酵母(Yarrowia lipolytica)
<400> 21
Met Val Ser Val Glu Glu Val Ala Lys His Asn Ser Lys Asp Ser Cys
1 5 10 15
Trp Val Ile Leu His Gly Lys Ala Tyr Asp Leu Thr Glu Phe Leu Pro
20 25 30
Glu His Pro Gly Gly Gln Ala Ile Ile Leu Lys Tyr Ala Gly Lys Asp
35 40 45
Ala Thr Lys Ala Phe Asp Pro Ile His Pro Arg Asp Val Val Asp Lys
50 55 60
Phe Leu Asp Lys Asp Lys His Leu Gly Glu Val Thr Gly Glu Ile Ala
65 70 75 80
Ala Glu Glu Glu Glu Glu Val Thr Pro Glu Glu Lys Ala Arg Leu Glu
85 90 95
Arg Tyr Glu Asn Arg Pro Pro Leu Ser Gln Ile Phe Asn Ser Phe Asp
100 105 110
Phe Glu Tyr Val Ala Arg His Thr Met Ser Pro Asn Ala Trp Ala Tyr
115 120 125
Tyr Ser Ser Gly Ser Asp Asp Glu Ile Thr Val Arg Glu Asn His Arg
130 135 140
Ala Phe His Lys Ile Trp Phe Arg Pro Arg Val Leu Val Asp Val Lys
145 150 155 160
Asn Val Asp Ile Ser Thr Thr Met Leu Gly Thr Lys Ser Ser Val Pro
165 170 175
Phe Tyr Ile Thr Ala Thr Ala Leu Gly Lys Leu Gly His Pro Glu Gly
180 185 190
Glu Val Val Leu Thr Arg Gly Ala Asp Lys Met Asp Val Ile Gln Met
195 200 205
Ile Pro Thr Leu Ala Ser Cys Ser Phe Asp Glu Ile Val Asp Ala Ala
210 215 220
Thr Asp Lys Gln Thr Gln Trp Met Gln Leu Tyr Val Asn Met Asp Arg
225 230 235 240
Glu Val Thr Lys Lys Ile Val Gln His Ala Glu Lys Arg Gly Val Lys
245 250 255
Gly Leu Phe Ile Thr Val Asp Ala Pro Gln Leu Gly Arg Arg Glu Lys
260 265 270
Asp Met Arg Thr Lys Phe Gly Asp Pro Gly Ala Gln Val Gln Gln Ser
275 280 285
Asp Asp Ser Val Asp Arg Ser Gln Gly Ala Ala Arg Ala Ile Ser Ser
290 295 300
Phe Ile Asp Pro Ser Leu Ser Trp Lys Asp Ile Pro Trp Phe Gln Ser
305 310 315 320
Ile Thr Lys Met Pro Ile Ile Leu Lys Gly Val Gln Cys Ala Glu Asp
325 330 335
Ala Leu Lys Ala Val Glu Tyr Lys Val Asp Gly Ile Leu Leu Ser Asn
340 345 350
His Gly Gly Arg Gln Leu Glu Phe Ala Arg Pro Ser Ile Glu Val Leu
355 360 365
Val Glu Val Met Ala Ala Leu Arg Ala Lys Gly Trp Gln Asp Tyr Ile
370 375 380
Glu Val Tyr Ile Asp Gly Gly Ile Arg Arg Ala Thr Asp Val Ile Lys
385 390 395 400
Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Ile Gly Arg Pro Phe Leu
405 410 415
Tyr Ala Met Ser Thr Tyr Gly Glu Asp Gly Val Cys His Leu Ile Gln
420 425 430
Leu Leu Lys Asp Glu Met Glu Met Asn Met Arg Leu Ile Gly Ala Thr
435 440 445
Lys Ile Glu Asp Leu Asn Pro Ser Met Val Asp Leu Lys Ser Ile Phe
450 455 460
Thr His Ser Ala Asp Thr Ala Arg Asp Ile Leu Gly Glu Asn Val Tyr
465 470 475 480
Gln His Met Glu Met Pro Leu Phe Lys Gly
485 490
<210> 22
<211> 2919
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 22
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctagt aaacaggccc cttttccttt 1560
gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc cacatccgct 1620
ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 1680
gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 1740
cgcgtgtacg catgtaacgg gcagacggcc ggccataact tcgtataatg tatgctatac 1800
gaagttatgg caacggttca tcatctcatg gatctgcaca tgaacaaaca ccagagtcaa 1860
acgacgttga aattgaggct actgcgccaa ttgatgacaa tacagacgat gataacaaac 1920
cgaagttatc tgatgtagaa aaggattaga gatgctaaga gatagtgatg atatttcata 1980
aataatgtaa ttctatatat gttaattacc ttttttgcga ggcatattta tggtgaagga 2040
taagttttga ccatcaaaga aggttaatgt ggctgtggtt tcagggtcca taaagctttt 2100
caattcatct tttttttttt tgttcttttt tttgattccg gtttctttga aatttttttg 2160
attcggtaat ctccgagcag aaggaagaac gaaggaagga gcacagactt agattggtat 2220
atatacgcat atgtggtgtt gaagaaacat gaaattgccc agtattctta acccaactgc 2280
acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac 2340
gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc 2400
aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg 2460
aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt 2520
ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct 2580
tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg 2640
tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta 2700
ttgttagcgg tttgaagcag gcggcggaag aagtaacaaa ggaacctaga ggccttttga 2760
tgttagcaga attgtcatgc aagggctccc tagctactgg agaatatact aagggtactg 2820
ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg 2880
gtggaagaga tgaaggttac gattggttga ttatgacac 2919
<210> 23
<211> 4700
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和东方伊萨酵母。
<400> 23
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgtctg atttagagtc acaacataca 1740
cgagaagagc tcgataagtc cgaggatgag gaaatccaac tcatcaatga aagccggcca 1800
ccaaagttgg ataaagagac gatcaagcgg tatctgaaaa ccagattcac tgagctattg 1860
ccaacaaagc aacagatgaa acaaaataaa catttgttga atccattgcc aggcttgaga 1920
atgatgggtc gtagacaatg gctgatgttt ctgagtgcct tctgcgcatg gacctgggac 1980
gcgtatgatt tctttacagt gtctctaaac accgtacaat tggccaaaga ttttgataag 2040
aacgtcaagg acattacatg ggggattaca cttgtgttga tgctaagatc agtggggggt 2100
atcacttttg gttatttggg tgataggtat ggtagaaaat ggcctttggt tgcaaacttg 2160
ttaattgttt gtattttaga aatcggaagc gggtttatca aaacctatag gcaatttcta 2220
ggcgtgagag cactatttgg tatttgttta gggggggttt atggtaatgc cactgccact 2280
gcattggatg attgtcctac agaagccaga gggttcattg gtgggttgtt gcaacagggt 2340
tatgcttttg gttatttgct tgccgttgta tttaaaagag ctattgctga caattcgtca 2400
cataagtgga gagctacctt ttggtttggt gctggtattt cctttctatt tgcacttttc 2460
agagcctctt tacctgaaac aaaggcattt ctaagaaaaa aggagattga gaggtacaac 2520
aaggaacacg ggatttatca gccaacattc caaatgaaaa ttgtggaatc gttgaagaac 2580
tactggttga tgatgatata ccttgttttg tttatgtcgg gattctcatt tatgtcacat 2640
tcatcacaag atttgtatcc taccctattg actgtgagat actccttttc agagaacaag 2700
gccacagtga ccaattgcgt tgccaatatc ggtgcattta ttggcggtgt atttaccggc 2760
catatctcta actttttggg cagacggttg acgatcatgt tgatatgcat ctttggaggt 2820
gcgttgattt acccttgggc ttttattgat ggcacaggta tcaatgcagg agttttcttt 2880
ctacaatggt gtgtgcaagg tggactaggg gtcgtgcctt cgcatttatc ggagttagca 2940
ccacctgatt tacgtgcatt tgtcgttgga atcagttatc aattaggtaa ccttgccgct 3000
tcagcctctt cgacaatcga aacgacaatt ggtgaacagt tcccaatgac ttcaccaagt 3060
ggcgaaccca tctacgacta cgcaaaagtc atggccatat ttgtaggttg cgtctttgct 3120
tttgtcctta tagtcacctt atttggccca gagagaagga atgcatcctt tgagaatgct 3180
gtaactacta atgaggagtt cggcaattcc gccggagccg ttgacaagat tgagcacgtg 3240
gaagaatgca ttgttccaaa gcacgctcgt aaccagcaat gttaattaat ttaccagctt 3300
actatccttc ttgaaaatat gcactctata tcttttagtt cttaattgca acacatagat 3360
ttgctgtata acgaatttta tgctattttt ttaatttgga gttcggtgat gaaagtgtca 3420
cagcgaattt cctcacatgt agggaccgaa ttgtttacaa gttctctgta ccaccatgga 3480
gacatcaaag attgaaaatc tatggaaaga tatggacggt agcaacaaga atatagcacg 3540
agccgcggag ttcatttcgt tacttttgat atcgctcaca actattgcga agcgcttcag 3600
tgaaaaaatc ataaggaaaa gttgtaaata ttattggtag tattcgtttg gtaaagtaga 3660
gggggtaatt tttccccttt attttgttca tacattctta aattgctttg cctctccttt 3720
tggaaagcta tacttcggag cactgttgag cgaaggctca ggccggcata tgacgtttta 3780
ttacctttga tcacatttcc acgccatttc gcattctcac cctcataagt catacaccga 3840
aaagaaagtt taagggatca atgagcttac tataatctca gtatatttat ttttatcgat 3900
gattcaccac aacaatcttg ctcccgaaaa gaaagcagac ggagtagaag catttgaaac 3960
tccttcagac cttcaagtat atatatatat atatatatat gtatatgtgt acattttcac 4020
gctaatacta atgtataatt agaagataat ttttactcat ttttcgttat cttcacgtca 4080
cccgaaccta gaaccaaatg tcattttcac gatatgtaaa tagtgaaata ggcaaaaacg 4140
ccaaaaagta gtaagcgcaa catacactaa accattaaag aatatctcga ccagaatcta 4200
acagatatac atgttccgat aatgtctgag ttaggtgagt attctaaatt agaaaacaaa 4260
gagcttagaa cggagtttga attgacaaat tttccttttc caggcacaac tgataacgac 4320
tccgatgacg gaagccaagg gcagaactct ttgaatatca ttactcctga catggatgat 4380
actctggtta atgatgtact tcgagaaaac gataaaaagt ctagtatgag aatggctttt 4440
atgaatctag caaactctat tcttggtgcc ggaataatta ctcagccgtt cgcgatcaaa 4500
aatgctggta tattaggcgg gctattatca tacgtagccc tcggatttat agttgattgg 4560
acgttaagac ttattgtcat taacttgact cttgctggca agagaacata ccagggtacg 4620
gtcgaacatg taatgggtaa aaaagggaaa ttgctgattc tatttacaaa cgggttattt 4680
gcatttggtg gatgtattgg 4700
<210> 24
<211> 523
<212> PRT
<213> 东方伊萨酵母(Issatchenkia orientalis)
<400> 24
Met Ser Asp Leu Glu Ser Gln His Thr Arg Glu Glu Leu Asp Lys Ser
1 5 10 15
Glu Asp Glu Glu Ile Gln Leu Ile Asn Glu Ser Arg Pro Pro Lys Leu
20 25 30
Asp Lys Glu Thr Ile Lys Arg Tyr Leu Lys Thr Arg Phe Thr Glu Leu
35 40 45
Leu Pro Thr Lys Gln Gln Met Lys Gln Asn Lys His Leu Leu Asn Pro
50 55 60
Leu Pro Gly Leu Arg Met Met Gly Arg Arg Gln Trp Leu Met Phe Leu
65 70 75 80
Ser Ala Phe Cys Ala Trp Thr Trp Asp Ala Tyr Asp Phe Phe Thr Val
85 90 95
Ser Leu Asn Thr Val Gln Leu Ala Lys Asp Phe Asp Lys Asn Val Lys
100 105 110
Asp Ile Thr Trp Gly Ile Thr Leu Val Leu Met Leu Arg Ser Val Gly
115 120 125
Gly Ile Thr Phe Gly Tyr Leu Gly Asp Arg Tyr Gly Arg Lys Trp Pro
130 135 140
Leu Val Ala Asn Leu Leu Ile Val Cys Ile Leu Glu Ile Gly Ser Gly
145 150 155 160
Phe Ile Lys Thr Tyr Arg Gln Phe Leu Gly Val Arg Ala Leu Phe Gly
165 170 175
Ile Cys Leu Gly Gly Val Tyr Gly Asn Ala Thr Ala Thr Ala Leu Asp
180 185 190
Asp Cys Pro Thr Glu Ala Arg Gly Phe Ile Gly Gly Leu Leu Gln Gln
195 200 205
Gly Tyr Ala Phe Gly Tyr Leu Leu Ala Val Val Phe Lys Arg Ala Ile
210 215 220
Ala Asp Asn Ser Ser His Lys Trp Arg Ala Thr Phe Trp Phe Gly Ala
225 230 235 240
Gly Ile Ser Phe Leu Phe Ala Leu Phe Arg Ala Ser Leu Pro Glu Thr
245 250 255
Lys Ala Phe Leu Arg Lys Lys Glu Ile Glu Arg Tyr Asn Lys Glu His
260 265 270
Gly Ile Tyr Gln Pro Thr Phe Gln Met Lys Ile Val Glu Ser Leu Lys
275 280 285
Asn Tyr Trp Leu Met Met Ile Tyr Leu Val Leu Phe Met Ser Gly Phe
290 295 300
Ser Phe Met Ser His Ser Ser Gln Asp Leu Tyr Pro Thr Leu Leu Thr
305 310 315 320
Val Arg Tyr Ser Phe Ser Glu Asn Lys Ala Thr Val Thr Asn Cys Val
325 330 335
Ala Asn Ile Gly Ala Phe Ile Gly Gly Val Phe Thr Gly His Ile Ser
340 345 350
Asn Phe Leu Gly Arg Arg Leu Thr Ile Met Leu Ile Cys Ile Phe Gly
355 360 365
Gly Ala Leu Ile Tyr Pro Trp Ala Phe Ile Asp Gly Thr Gly Ile Asn
370 375 380
Ala Gly Val Phe Phe Leu Gln Trp Cys Val Gln Gly Gly Leu Gly Val
385 390 395 400
Val Pro Ser His Leu Ser Glu Leu Ala Pro Pro Asp Leu Arg Ala Phe
405 410 415
Val Val Gly Ile Ser Tyr Gln Leu Gly Asn Leu Ala Ala Ser Ala Ser
420 425 430
Ser Thr Ile Glu Thr Thr Ile Gly Glu Gln Phe Pro Met Thr Ser Pro
435 440 445
Ser Gly Glu Pro Ile Tyr Asp Tyr Ala Lys Val Met Ala Ile Phe Val
450 455 460
Gly Cys Val Phe Ala Phe Val Leu Ile Val Thr Leu Phe Gly Pro Glu
465 470 475 480
Arg Arg Asn Ala Ser Phe Glu Asn Ala Val Thr Thr Asn Glu Glu Phe
485 490 495
Gly Asn Ser Ala Gly Ala Val Asp Lys Ile Glu His Val Glu Glu Cys
500 505 510
Ile Val Pro Lys His Ala Arg Asn Gln Gln Cys
515 520
<210> 25
<211> 4979
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 25
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgtcgt cgtcaattac agatgagaaa 1740
atatctggtg aacagcaaca acctgctggc agaaaactat actataacac aagtacattt 1800
gcagagcctc ctctagtgga cggagaaggt aaccctataa attatgagcc ggaagtttac 1860
aacccggatc acgaaaagct ataccataac ccatcactgc ctgcacaatc aattcaggat 1920
acaagagatg atgaattgct ggaaagagtt tatagccagg atcaaggtgt agagtatgag 1980
gaagatgaag aggataagcc aaacctaagc gctgcgtcca ttaaaagtta tgctttaacg 2040
agatttacgt ccttactgca catccacgag ttttcttggg agaatgtcaa tcccataccc 2100
gaactgcgca aaatgacatg gcagaattgg aactattttt ttatgggtta ttttgcgtgg 2160
ttgtctgcgg cttgggcctt cttttgcgtt tcagtatcag tcgctccatt ggctgaacta 2220
tatgacagac caaccaagga catcacctgg gggttgggat tggtgttatt tgttcgttca 2280
gcaggtgctg tcatatttgg tttatggaca gataagtctt ccagaaagtg gccgtacatt 2340
acatgtttgt tcttatttgt cattgcacaa ctctgtactc catggtgtga cacatacgag 2400
aaatttctgg gcgtaaggtg gataaccggt attgctatgg gaggaattta cggatgtgct 2460
tctgcaacag cgattgaaga tgcacctgtg aaagcacgtt cgttcctatc aggtctattt 2520
ttttctgctt acgctatggg gttcatattt gctatcattt tttacagagc ctttggctac 2580
tttagggatg atggctggaa aatattgttt tggtttagta tttttctacc aattctacta 2640
attttctgga gattgttatg gcctgaaacg aaatacttca ccaaggtttt gaaagcccgt 2700
aaattaatat tgagtgacgc agtgaaagct aatggtggcg agcctctacc aaaagccaac 2760
tttaaacaaa agatggtatc catgaagaga acagttcaaa agtactggtt gttgttcgca 2820
tatttggttg ttttattggt gggtccaaat tacttgactc atgcttctca agacttgttg 2880
ccaaccatgc tgcgtgccca attaggccta tccaaggatg ctgtcactgt cattgtagtg 2940
gttaccaaca tcggtgctat ttgtgggggt atgatatttg gacagttcat ggaagttact 3000
ggaagaagat taggcctatt gattgcatgc acaatgggtg gttgcttcac ctaccctgca 3060
tttatgttga gaagcgaaaa ggctatatta ggtgccggtt tcatgttata tttttgtgtc 3120
tttggtgtct ggggtatcct gcccattcac cttgcagagt tggcccctgc tgatgcaagg 3180
gctttggttg ccggtttatc ttaccagcta ggtaatctag cttctgcagc ggcttccacg 3240
attgagacac agttagctga tagataccca ttagaaagag atgcctctgg tgctgtgatt 3300
aaagaagatt atgccaaagt tatggctatc ttgactggtt ctgttttcat cttcacattt 3360
gcttgtgttt ttgttggcca tgagaaattc catcgtgatt tgtcctctcc tgttatgaag 3420
aaatatataa accaagtgga agaatacgaa gccgatggtc tttcgattag tgacattgtt 3480
gaacaaaaga cggaatgtgc ttcagtgaag atgattgatt cgaacgtctc aaagacatat 3540
gaggagcata ttgagaccgt ttaattaatt taccagctta ctatccttct tgaaaatatg 3600
cactctatat cttttagttc ttaattgcaa cacatagatt tgctgtataa cgaattttat 3660
gctatttttt taatttggag ttcggtgatg aaagtgtcac agcgaatttc ctcacatgta 3720
gggaccgaat tgtttacaag ttctctgtac caccatggag acatcaaaga ttgaaaatct 3780
atggaaagat atggacggta gcaacaagaa tatagcacga gccgcggagt tcatttcgtt 3840
acttttgata tcgctcacaa ctattgcgaa gcgcttcagt gaaaaaatca taaggaaaag 3900
ttgtaaatat tattggtagt attcgtttgg taaagtagag ggggtaattt ttccccttta 3960
ttttgttcat acattcttaa attgctttgc ctctcctttt ggaaagctat acttcggagc 4020
actgttgagc gaaggctcag gccggcatat gacgttttat tacctttgat cacatttcca 4080
cgccatttcg cattctcacc ctcataagtc atacaccgaa aagaaagttt aagggatcaa 4140
tgagcttact ataatctcag tatatttatt tttatcgatg attcaccaca acaatcttgc 4200
tcccgaaaag aaagcagacg gagtagaagc atttgaaact ccttcagacc ttcaagtata 4260
tatatatata tatatatatg tatatgtgta cattttcacg ctaatactaa tgtataatta 4320
gaagataatt tttactcatt tttcgttatc ttcacgtcac ccgaacctag aaccaaatgt 4380
cattttcacg atatgtaaat agtgaaatag gcaaaaacgc caaaaagtag taagcgcaac 4440
atacactaaa ccattaaaga atatctcgac cagaatctaa cagatataca tgttccgata 4500
atgtctgagt taggtgagta ttctaaatta gaaaacaaag agcttagaac ggagtttgaa 4560
ttgacaaatt ttccttttcc aggcacaact gataacgact ccgatgacgg aagccaaggg 4620
cagaactctt tgaatatcat tactcctgac atggatgata ctctggttaa tgatgtactt 4680
cgagaaaacg ataaaaagtc tagtatgaga atggctttta tgaatctagc aaactctatt 4740
cttggtgccg gaataattac tcagccgttc gcgatcaaaa atgctggtat attaggcggg 4800
ctattatcat acgtagccct cggatttata gttgattgga cgttaagact tattgtcatt 4860
aacttgactc ttgctggcaa gagaacatac cagggtacgg tcgaacatgt aatgggtaaa 4920
aaagggaaat tgctgattct atttacaaac gggttatttg catttggtgg atgtattgg 4979
<210> 26
<211> 616
<212> PRT
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 26
Met Ser Ser Ser Ile Thr Asp Glu Lys Ile Ser Gly Glu Gln Gln Gln
1 5 10 15
Pro Ala Gly Arg Lys Leu Tyr Tyr Asn Thr Ser Thr Phe Ala Glu Pro
20 25 30
Pro Leu Val Asp Gly Glu Gly Asn Pro Ile Asn Tyr Glu Pro Glu Val
35 40 45
Tyr Asn Pro Asp His Glu Lys Leu Tyr His Asn Pro Ser Leu Pro Ala
50 55 60
Gln Ser Ile Gln Asp Thr Arg Asp Asp Glu Leu Leu Glu Arg Val Tyr
65 70 75 80
Ser Gln Asp Gln Gly Val Glu Tyr Glu Glu Asp Glu Glu Asp Lys Pro
85 90 95
Asn Leu Ser Ala Ala Ser Ile Lys Ser Tyr Ala Leu Thr Arg Phe Thr
100 105 110
Ser Leu Leu His Ile His Glu Phe Ser Trp Glu Asn Val Asn Pro Ile
115 120 125
Pro Glu Leu Arg Lys Met Thr Trp Gln Asn Trp Asn Tyr Phe Phe Met
130 135 140
Gly Tyr Phe Ala Trp Leu Ser Ala Ala Trp Ala Phe Phe Cys Val Ser
145 150 155 160
Val Ser Val Ala Pro Leu Ala Glu Leu Tyr Asp Arg Pro Thr Lys Asp
165 170 175
Ile Thr Trp Gly Leu Gly Leu Val Leu Phe Val Arg Ser Ala Gly Ala
180 185 190
Val Ile Phe Gly Leu Trp Thr Asp Lys Ser Ser Arg Lys Trp Pro Tyr
195 200 205
Ile Thr Cys Leu Phe Leu Phe Val Ile Ala Gln Leu Cys Thr Pro Trp
210 215 220
Cys Asp Thr Tyr Glu Lys Phe Leu Gly Val Arg Trp Ile Thr Gly Ile
225 230 235 240
Ala Met Gly Gly Ile Tyr Gly Cys Ala Ser Ala Thr Ala Ile Glu Asp
245 250 255
Ala Pro Val Lys Ala Arg Ser Phe Leu Ser Gly Leu Phe Phe Ser Ala
260 265 270
Tyr Ala Met Gly Phe Ile Phe Ala Ile Ile Phe Tyr Arg Ala Phe Gly
275 280 285
Tyr Phe Arg Asp Asp Gly Trp Lys Ile Leu Phe Trp Phe Ser Ile Phe
290 295 300
Leu Pro Ile Leu Leu Ile Phe Trp Arg Leu Leu Trp Pro Glu Thr Lys
305 310 315 320
Tyr Phe Thr Lys Val Leu Lys Ala Arg Lys Leu Ile Leu Ser Asp Ala
325 330 335
Val Lys Ala Asn Gly Gly Glu Pro Leu Pro Lys Ala Asn Phe Lys Gln
340 345 350
Lys Met Val Ser Met Lys Arg Thr Val Gln Lys Tyr Trp Leu Leu Phe
355 360 365
Ala Tyr Leu Val Val Leu Leu Val Gly Pro Asn Tyr Leu Thr His Ala
370 375 380
Ser Gln Asp Leu Leu Pro Thr Met Leu Arg Ala Gln Leu Gly Leu Ser
385 390 395 400
Lys Asp Ala Val Thr Val Ile Val Val Val Thr Asn Ile Gly Ala Ile
405 410 415
Cys Gly Gly Met Ile Phe Gly Gln Phe Met Glu Val Thr Gly Arg Arg
420 425 430
Leu Gly Leu Leu Ile Ala Cys Thr Met Gly Gly Cys Phe Thr Tyr Pro
435 440 445
Ala Phe Met Leu Arg Ser Glu Lys Ala Ile Leu Gly Ala Gly Phe Met
450 455 460
Leu Tyr Phe Cys Val Phe Gly Val Trp Gly Ile Leu Pro Ile His Leu
465 470 475 480
Ala Glu Leu Ala Pro Ala Asp Ala Arg Ala Leu Val Ala Gly Leu Ser
485 490 495
Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ala Ser Thr Ile Glu Thr
500 505 510
Gln Leu Ala Asp Arg Tyr Pro Leu Glu Arg Asp Ala Ser Gly Ala Val
515 520 525
Ile Lys Glu Asp Tyr Ala Lys Val Met Ala Ile Leu Thr Gly Ser Val
530 535 540
Phe Ile Phe Thr Phe Ala Cys Val Phe Val Gly His Glu Lys Phe His
545 550 555 560
Arg Asp Leu Ser Ser Pro Val Met Lys Lys Tyr Ile Asn Gln Val Glu
565 570 575
Glu Tyr Glu Ala Asp Gly Leu Ser Ile Ser Asp Ile Val Glu Gln Lys
580 585 590
Thr Glu Cys Ala Ser Val Lys Met Ile Asp Ser Asn Val Ser Lys Thr
595 600 605
Tyr Glu Glu His Ile Glu Thr Val
610 615
<210> 27
<211> 4935
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和乳酸克鲁维酵母()。
<400> 27
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgaata acaacaacat tacaccagag 1740
tcagactcaa tgaagagtaa cgataacgat caaaccaatg attacatgcc agatgtggct 1800
gatttcgatc atacacaaac aaacacaaac gaaattgcta gagcaatttc tcacccaggt 1860
tctgttctgt caagagttgc aagctacgtc agcagaaagg acagatacgt agacgagaat 1920
ggcaatgaag tttggcaaga cgatgaagtg agtatcctaa tggaggaaga tgagactcct 1980
gatttcacat ggaaaaacat tagacattac gctatcacta gattcactac attgacagaa 2040
cttcacagag tgtctatgga aaacatcaac ccaattccag aattgagaaa aatgacatta 2100
cataattgga actacttctt tatggggtat gccgcgtggt tgtgtgctgc ttgggcattt 2160
ttcgccgttt ccgttagtac agcccctctt gcaacccttt atggcaagga aacaaaagac 2220
atttcatggg gtctatcctt agtcttattc gtcagatctg ctggagcgat tatctttggc 2280
atctggaccg acaattactc cagaaagtgg ccatacatta catgcttagg tcttttcttg 2340
atctgtcagc tttgcacacc ttgggcaaag acctacactc aattcttggg agttcgttgg 2400
atttccggta tcgcaatggg tggaatttac gcctgtgctt ctgcaacagc gattgaggat 2460
gcaccagtaa aggctcgttc tttcttatcc ggcttattct tcactgccta cgccatgggt 2520
tttatcttcg caataatctt ttacagagca ttcttaaacg tcaacggtga aaactactgg 2580
aaggttcaat tctggttttc aatatggtta ccagctgtac tgatattgtg gagactcgta 2640
tggccagaaa ctaagtactt caccaaagta ctaaaagcta gacaactaat gcgtgatgat 2700
gcaatagcca aaaacggtgg tcaaccactc ccaaagttgt ctttcaagca aaagttcgcc 2760
aatgtgaaaa agaccgttag taaatactgg cttttgtttg gttacttaat tctcttgttg 2820
gttggtccta actacctaac acacgcctct caagacttgt ttcctacaat gttaagagca 2880
caattgagat tttctgaaga tgctgtcacc gtggccattg tagttgtttg tctgggtagc 2940
attgctgggg ggatgttttt cggacagttg atggagatca ctggtagaag agttggccta 3000
ctattggccc taatcatggc tggctgcttt acttaccctg ccttcatgct taaaacttca 3060
tctgcagtgc tgggggcagg ctttatgctc tggttctcaa tcttaggtgt gtggggtgtt 3120
ttaccaatcc atttatcaga attgtcccct ccagaagcta gagcattagt ctctggatta 3180
gcatatcagc tgggtaatct agcttcagca gcttctgttg ttatagagaa tgatttggcg 3240
gatttgtatc caatagaatg gaattctgct ggagaagtta caaacaagga ctactctaag 3300
gtaatggcta ttcttacagg ttcttcagtc attttcactt tcgtgttggt tttcgttggt 3360
cacgaaaagt ttcatagaga tttgtcatcc ccacatctta agtcatacat cgagagagtc 3420
gatcaaacag aagaggtcgc agctatgact ggatctactg cgaactctat ctctagtaag 3480
ccttcagatg atcagctcga aaaagtttca gtctagttaa ttaatttacc agcttactat 3540
ccttcttgaa aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct 3600
gtataacgaa ttttatgcta tttttttaat ttggagttcg gtgatgaaag tgtcacagcg 3660
aatttcctca catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat 3720
caaagattga aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg 3780
cggagttcat ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa 3840
aaatcataag gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg 3900
taatttttcc cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa 3960
agctatactt cggagcactg ttgagcgaag gctcaggccg gcatatgacg ttttattacc 4020
tttgatcaca tttccacgcc atttcgcatt ctcaccctca taagtcatac accgaaaaga 4080
aagtttaagg gatcaatgag cttactataa tctcagtata tttattttta tcgatgattc 4140
accacaacaa tcttgctccc gaaaagaaag cagacggagt agaagcattt gaaactcctt 4200
cagaccttca agtatatata tatatatata tatatgtata tgtgtacatt ttcacgctaa 4260
tactaatgta taattagaag ataattttta ctcatttttc gttatcttca cgtcacccga 4320
acctagaacc aaatgtcatt ttcacgatat gtaaatagtg aaataggcaa aaacgccaaa 4380
aagtagtaag cgcaacatac actaaaccat taaagaatat ctcgaccaga atctaacaga 4440
tatacatgtt ccgataatgt ctgagttagg tgagtattct aaattagaaa acaaagagct 4500
tagaacggag tttgaattga caaattttcc ttttccaggc acaactgata acgactccga 4560
tgacggaagc caagggcaga actctttgaa tatcattact cctgacatgg atgatactct 4620
ggttaatgat gtacttcgag aaaacgataa aaagtctagt atgagaatgg cttttatgaa 4680
tctagcaaac tctattcttg gtgccggaat aattactcag ccgttcgcga tcaaaaatgc 4740
tggtatatta ggcgggctat tatcatacgt agccctcgga tttatagttg attggacgtt 4800
aagacttatt gtcattaact tgactcttgc tggcaagaga acataccagg gtacggtcga 4860
acatgtaatg ggtaaaaaag ggaaattgct gattctattt acaaacgggt tatttgcatt 4920
tggtggatgt attgg 4935
<210> 28
<211> 600
<212> PRT
<213> 乳酸克鲁维酵母(Kluyveromyces lactis)
<400> 28
Met Asn Asn Asn Asn Ile Thr Pro Glu Ser Asp Ser Met Lys Ser Asn
1 5 10 15
Asp Asn Asp Gln Thr Asn Asp Tyr Met Pro Asp Val Ala Asp Phe Asp
20 25 30
His Thr Gln Thr Asn Thr Asn Glu Ile Ala Arg Ala Ile Ser His Pro
35 40 45
Gly Ser Val Leu Ser Arg Val Ala Ser Tyr Val Ser Arg Lys Asp Arg
50 55 60
Tyr Val Asp Glu Asn Gly Asn Glu Val Trp Gln Asp Asp Glu Val Ser
65 70 75 80
Ile Leu Met Glu Glu Asp Glu Thr Pro Asp Phe Thr Trp Lys Asn Ile
85 90 95
Arg His Tyr Ala Ile Thr Arg Phe Thr Thr Leu Thr Glu Leu His Arg
100 105 110
Val Ser Met Glu Asn Ile Asn Pro Ile Pro Glu Leu Arg Lys Met Thr
115 120 125
Leu His Asn Trp Asn Tyr Phe Phe Met Gly Tyr Ala Ala Trp Leu Cys
130 135 140
Ala Ala Trp Ala Phe Phe Ala Val Ser Val Ser Thr Ala Pro Leu Ala
145 150 155 160
Thr Leu Tyr Gly Lys Glu Thr Lys Asp Ile Ser Trp Gly Leu Ser Leu
165 170 175
Val Leu Phe Val Arg Ser Ala Gly Ala Ile Ile Phe Gly Ile Trp Thr
180 185 190
Asp Asn Tyr Ser Arg Lys Trp Pro Tyr Ile Thr Cys Leu Gly Leu Phe
195 200 205
Leu Ile Cys Gln Leu Cys Thr Pro Trp Ala Lys Thr Tyr Thr Gln Phe
210 215 220
Leu Gly Val Arg Trp Ile Ser Gly Ile Ala Met Gly Gly Ile Tyr Ala
225 230 235 240
Cys Ala Ser Ala Thr Ala Ile Glu Asp Ala Pro Val Lys Ala Arg Ser
245 250 255
Phe Leu Ser Gly Leu Phe Phe Thr Ala Tyr Ala Met Gly Phe Ile Phe
260 265 270
Ala Ile Ile Phe Tyr Arg Ala Phe Leu Asn Val Asn Gly Glu Asn Tyr
275 280 285
Trp Lys Val Gln Phe Trp Phe Ser Ile Trp Leu Pro Ala Val Leu Ile
290 295 300
Leu Trp Arg Leu Val Trp Pro Glu Thr Lys Tyr Phe Thr Lys Val Leu
305 310 315 320
Lys Ala Arg Gln Leu Met Arg Asp Asp Ala Ile Ala Lys Asn Gly Gly
325 330 335
Gln Pro Leu Pro Lys Leu Ser Phe Lys Gln Lys Phe Ala Asn Val Lys
340 345 350
Lys Thr Val Ser Lys Tyr Trp Leu Leu Phe Gly Tyr Leu Ile Leu Leu
355 360 365
Leu Val Gly Pro Asn Tyr Leu Thr His Ala Ser Gln Asp Leu Phe Pro
370 375 380
Thr Met Leu Arg Ala Gln Leu Arg Phe Ser Glu Asp Ala Val Thr Val
385 390 395 400
Ala Ile Val Val Val Cys Leu Gly Ser Ile Ala Gly Gly Met Phe Phe
405 410 415
Gly Gln Leu Met Glu Ile Thr Gly Arg Arg Val Gly Leu Leu Leu Ala
420 425 430
Leu Ile Met Ala Gly Cys Phe Thr Tyr Pro Ala Phe Met Leu Lys Thr
435 440 445
Ser Ser Ala Val Leu Gly Ala Gly Phe Met Leu Trp Phe Ser Ile Leu
450 455 460
Gly Val Trp Gly Val Leu Pro Ile His Leu Ser Glu Leu Ser Pro Pro
465 470 475 480
Glu Ala Arg Ala Leu Val Ser Gly Leu Ala Tyr Gln Leu Gly Asn Leu
485 490 495
Ala Ser Ala Ala Ser Val Val Ile Glu Asn Asp Leu Ala Asp Leu Tyr
500 505 510
Pro Ile Glu Trp Asn Ser Ala Gly Glu Val Thr Asn Lys Asp Tyr Ser
515 520 525
Lys Val Met Ala Ile Leu Thr Gly Ser Ser Val Ile Phe Thr Phe Val
530 535 540
Leu Val Phe Val Gly His Glu Lys Phe His Arg Asp Leu Ser Ser Pro
545 550 555 560
His Leu Lys Ser Tyr Ile Glu Arg Val Asp Gln Thr Glu Glu Val Ala
565 570 575
Ala Met Thr Gly Ser Thr Ala Asn Ser Ile Ser Ser Lys Pro Ser Asp
580 585 590
Asp Gln Leu Glu Lys Val Ser Val
595 600
<210> 29
<211> 3981
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 29
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgtcag acaaagagca aacatctggt 1740
aataccgatt tagaaaacgc tccagcaggg tactacagct cccacgataa tgacgtcaac 1800
ggtgttgcag aagatgaaag accttcacat gattctttgg gtaagattta cactggaggt 1860
gataacaacg agtacatcta tatcgggaga caaaagtttt tgaaatctga cttgtatcaa 1920
gcattcggag ggactttgaa tcctggtctt gctccagcac ctgttcataa gttcgccaac 1980
ccagctccac ttggcttatc agcttttgct ctgacaacat tcgttttaag tatgttcaac 2040
gcaagagcac agggcattac agtgccaaac gttgttgtag gctgcgcaat gttctacggt 2100
ggtttagttc aactaattgc tggtatatgg gagatcgcac tagaaaacac ctttggtggc 2160
actgcgttgt gttcatacgg aggtttctgg ctttcttttg ccgctattta cattccttgg 2220
ttcggaatcc tggaagcata cgaggataac gaatcagatt tgaacaatgc cctaggtttc 2280
tacctcttag ggtgggcaat tttcacattt ggtttaactg tatgtacaat gaagtctacc 2340
gtcatgtttt tcctgctatt cttcttagtg gccttgacat tcttgctctt atctatcgga 2400
cactttgcca atagacttgg cgtgactaga gctggcggtg tgttgggagt tgtcgttgcc 2460
ttcataggtt ggtataatgc gtacgctggt gtagcgacta aacagaactc ctacgtcttg 2520
gctagaccat ttccacttcc aagtacagaa cgtgttatct tcttaattaa tttaccagct 2580
tactatcctt cttgaaaata tgcactctat atcttttagt tcttaattgc aacacataga 2640
tttgctgtat aacgaatttt atgctatttt tttaatttgg agttcggtga tgaaagtgtc 2700
acagcgaatt tcctcacatg tagggaccga attgtttaca agttctctgt accaccatgg 2760
agacatcaaa gattgaaaat ctatggaaag atatggacgg tagcaacaag aatatagcac 2820
gagccgcgga gttcatttcg ttacttttga tatcgctcac aactattgcg aagcgcttca 2880
gtgaaaaaat cataaggaaa agttgtaaat attattggta gtattcgttt ggtaaagtag 2940
agggggtaat ttttcccctt tattttgttc atacattctt aaattgcttt gcctctcctt 3000
ttggaaagct atacttcgga gcactgttga gcgaaggctc aggccggcat atgacgtttt 3060
attacctttg atcacatttc cacgccattt cgcattctca ccctcataag tcatacaccg 3120
aaaagaaagt ttaagggatc aatgagctta ctataatctc agtatattta tttttatcga 3180
tgattcacca caacaatctt gctcccgaaa agaaagcaga cggagtagaa gcatttgaaa 3240
ctccttcaga ccttcaagta tatatatata tatatatata tgtatatgtg tacattttca 3300
cgctaatact aatgtataat tagaagataa tttttactca tttttcgtta tcttcacgtc 3360
acccgaacct agaaccaaat gtcattttca cgatatgtaa atagtgaaat aggcaaaaac 3420
gccaaaaagt agtaagcgca acatacacta aaccattaaa gaatatctcg accagaatct 3480
aacagatata catgttccga taatgtctga gttaggtgag tattctaaat tagaaaacaa 3540
agagcttaga acggagtttg aattgacaaa ttttcctttt ccaggcacaa ctgataacga 3600
ctccgatgac ggaagccaag ggcagaactc tttgaatatc attactcctg acatggatga 3660
tactctggtt aatgatgtac ttcgagaaaa cgataaaaag tctagtatga gaatggcttt 3720
tatgaatcta gcaaactcta ttcttggtgc cggaataatt actcagccgt tcgcgatcaa 3780
aaatgctggt atattaggcg ggctattatc atacgtagcc ctcggattta tagttgattg 3840
gacgttaaga cttattgtca ttaacttgac tcttgctggc aagagaacat accagggtac 3900
ggtcgaacat gtaatgggta aaaaagggaa attgctgatt ctatttacaa acgggttatt 3960
tgcatttggt ggatgtattg g 3981
<210> 30
<211> 293
<212> PRT
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 30
Met Ser Asp Lys Glu Gln Thr Ser Gly Asn Thr Asp Leu Glu Asn Ala
1 5 10 15
Pro Ala Gly Tyr Tyr Ser Ser His Asp Asn Asp Val Asn Gly Val Ala
20 25 30
Glu Asp Glu Arg Pro Ser His Asp Ser Leu Gly Lys Ile Tyr Thr Gly
35 40 45
Gly Asp Asn Asn Glu Tyr Ile Tyr Ile Gly Arg Gln Lys Phe Leu Lys
50 55 60
Ser Asp Leu Tyr Gln Ala Phe Gly Gly Thr Leu Asn Pro Gly Leu Ala
65 70 75 80
Pro Ala Pro Val His Lys Phe Ala Asn Pro Ala Pro Leu Gly Leu Ser
85 90 95
Ala Phe Ala Leu Thr Thr Phe Val Leu Ser Met Phe Asn Ala Arg Ala
100 105 110
Gln Gly Ile Thr Val Pro Asn Val Val Val Gly Cys Ala Met Phe Tyr
115 120 125
Gly Gly Leu Val Gln Leu Ile Ala Gly Ile Trp Glu Ile Ala Leu Glu
130 135 140
Asn Thr Phe Gly Gly Thr Ala Leu Cys Ser Tyr Gly Gly Phe Trp Leu
145 150 155 160
Ser Phe Ala Ala Ile Tyr Ile Pro Trp Phe Gly Ile Leu Glu Ala Tyr
165 170 175
Glu Asp Asn Glu Ser Asp Leu Asn Asn Ala Leu Gly Phe Tyr Leu Leu
180 185 190
Gly Trp Ala Ile Phe Thr Phe Gly Leu Thr Val Cys Thr Met Lys Ser
195 200 205
Thr Val Met Phe Phe Leu Leu Phe Phe Leu Val Ala Leu Thr Phe Leu
210 215 220
Leu Leu Ser Ile Gly His Phe Ala Asn Arg Leu Gly Val Thr Arg Ala
225 230 235 240
Gly Gly Val Leu Gly Val Val Val Ala Phe Ile Gly Trp Tyr Asn Ala
245 250 255
Tyr Ala Gly Val Ala Thr Lys Gln Asn Ser Tyr Val Leu Ala Arg Pro
260 265 270
Phe Pro Leu Pro Ser Thr Glu Arg Val Ile Phe Leu Ile Asn Leu Pro
275 280 285
Ala Tyr Tyr Pro Ser
290
<210> 31
<211> 3921
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和放线共生放线杆菌。
<400> 31
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgaaagtgg cagtgtactc 1560
aacaaaatct tacgacagaa agtacctcga actgatagat gcgaagtacg gattggattt 1620
ggaattctac gacttcatgc tttctgaacg tactgctaag atggccgaac attgcgaagc 1680
cgtttgtatc tttgttaacg atgatggctc cagaaaggta cttgaaaagt tagctgcttt 1740
gggtgttaag ataattgcac ttagatgcgc tggatttaac aatgtggacc ttaaagcagc 1800
acaagagcta gggctaacag ttgtgagagt tccagcatac tcaccagagg cagtcgcaga 1860
acatacagtt ggattaatga tgaccctaaa tagacgtatt cacagagctt accagagaac 1920
aagagaagcc aatttctctt tagagggtct aatcgggttc aatatgtacg gtagaactgt 1980
tggtgtcatt ggtactggga agatcggtgt tgctgtgatg agaattttga agggtttcgg 2040
catgcatata ctggcttttg atccattcaa aaacccaact gctgaggaac tcggcgctga 2100
atatgtctct ttagatgaga tttacagaag atctcacgtt attacattgc attgtcctgc 2160
cacccctgaa aactatcatt tgttaaacag agaagccttt gctaaaatga aggatggagt 2220
catgatcatc aatacatcaa gaggtacact catagacacc aaggcggcca ttgacgccct 2280
aaagcaacgt aaaatcggtg cattaggtat ggatgtttac gagaacgaaa gagatttgtt 2340
cttcgaggat aagtcaaacg aagtaatcct ggacgatgta ttcagaagat taagtagctg 2400
ccataacgtc ttattgacag gacaccaagc gtttttgaca gaggaagcat tgacttccat 2460
tgctgatgta actcttagta acatttacgc aatcggtaaa ggcaagccat gtgataacgt 2520
cgttttgtca tcttagttaa ttaaacaggc cccttttcct ttgtcgatat catgtaatta 2580
gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga 2640
gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 2700
acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac 2760
gggcagacgg ccggccataa cttcgtataa tgtatgctat acgaagttat ggcaacggtt 2820
catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt gaaattgagg 2880
ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta tctgatgtag 2940
aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt aattctatat 3000
atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa 3060
gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat cttttttttt 3120
tttgttcttt tttttgattc cggtttcttt gaaatttttt tgattcggta atctccgagc 3180
agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtggtg 3240
ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca 3300
ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta 3360
gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt 3420
cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa 3480
tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta 3540
agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg 3600
ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat 3660
gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc 3720
aggcggcgga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat 3780
gcaagggctc cctagctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg 3840
acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt 3900
acgattggtt gattatgaca c 3921
<210> 32
<211> 331
<212> PRT
<213> 放线共生放线杆菌(Aggregatibacter actinomycetemcomitans)
<400> 32
Met Lys Val Ala Val Tyr Ser Thr Lys Ser Tyr Asp Arg Lys Tyr Leu
1 5 10 15
Glu Leu Ile Asp Ala Lys Tyr Gly Leu Asp Leu Glu Phe Tyr Asp Phe
20 25 30
Met Leu Ser Glu Arg Thr Ala Lys Met Ala Glu His Cys Glu Ala Val
35 40 45
Cys Ile Phe Val Asn Asp Asp Gly Ser Arg Lys Val Leu Glu Lys Leu
50 55 60
Ala Ala Leu Gly Val Lys Ile Ile Ala Leu Arg Cys Ala Gly Phe Asn
65 70 75 80
Asn Val Asp Leu Lys Ala Ala Gln Glu Leu Gly Leu Thr Val Val Arg
85 90 95
Val Pro Ala Tyr Ser Pro Glu Ala Val Ala Glu His Thr Val Gly Leu
100 105 110
Met Met Thr Leu Asn Arg Arg Ile His Arg Ala Tyr Gln Arg Thr Arg
115 120 125
Glu Ala Asn Phe Ser Leu Glu Gly Leu Ile Gly Phe Asn Met Tyr Gly
130 135 140
Arg Thr Val Gly Val Ile Gly Thr Gly Lys Ile Gly Val Ala Val Met
145 150 155 160
Arg Ile Leu Lys Gly Phe Gly Met His Ile Leu Ala Phe Asp Pro Phe
165 170 175
Lys Asn Pro Thr Ala Glu Glu Leu Gly Ala Glu Tyr Val Ser Leu Asp
180 185 190
Glu Ile Tyr Arg Arg Ser His Val Ile Thr Leu His Cys Pro Ala Thr
195 200 205
Pro Glu Asn Tyr His Leu Leu Asn Arg Glu Ala Phe Ala Lys Met Lys
210 215 220
Asp Gly Val Met Ile Ile Asn Thr Ser Arg Gly Thr Leu Ile Asp Thr
225 230 235 240
Lys Ala Ala Ile Asp Ala Leu Lys Gln Arg Lys Ile Gly Ala Leu Gly
245 250 255
Met Asp Val Tyr Glu Asn Glu Arg Asp Leu Phe Phe Glu Asp Lys Ser
260 265 270
Asn Glu Val Ile Leu Asp Asp Val Phe Arg Arg Leu Ser Ser Cys His
275 280 285
Asn Val Leu Leu Thr Gly His Gln Ala Phe Leu Thr Glu Glu Ala Leu
290 295 300
Thr Ser Ile Ala Asp Val Thr Leu Ser Asn Ile Tyr Ala Ile Gly Lys
305 310 315 320
Gly Lys Pro Cys Asp Asn Val Val Leu Ser Ser
325 330
<210> 33
<211> 3924
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和鸭嘴兽
<400> 33
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atggctggtg ttaaagagca 1560
attgatccaa aacttactaa aggaagagta cgcacctcaa aacaagataa cagttgtggg 1620
ggttggagct gttggtatgg catgtgccat ttctatccta atgaaagatc tggctgatga 1680
gcttgccttg gtcgacgtaa tcgaagataa gctgaaggga gaaatgatgg atttacaaca 1740
cggttctctt ttcttgagaa caccaaagat agtctccggc aaagattaca gcgtgaccgc 1800
aaacagcaag ttagtgatta tcacagcggg agcaagacag caggaaggtg aatcaagatt 1860
gaatcttgtg caacgtaacg ttaacatatt caagtttatc atcccaaatg ttgttaagta 1920
cagtccaaat tgcaagttgt tggttgtttc caatcctgtt gatattctta cttatgttgc 1980
atggaaaatc tcaggttttc ctaaaaacag agtcataggt tcaggttgta acctagattc 2040
tgccagattc agatacttaa tgggtgaaag attaggcatt cattctactt cctgccatgg 2100
gtgggtgatt ggtgaacatg gagactcctc agttccagtg tggtctggtg tcaacgtagc 2160
gggcgttagt ttgaaaaacc ttcaccctga tttggggact gacgcagaca aggaacagtg 2220
gaaagatgtt cataagcaag tagtagattc tgcttacgaa gtcattaagt taaagggcta 2280
cacatcttgg gctattggtt tgagtgtcgc tgatctggca gagtcaatcg tcaaaaactt 2340
aagacgtgta cacccaatct ctacaatgat taaaggctta tacggtatta aggacgaagt 2400
ttttctatca gtaccatgtg ttctcggaca aaatggaatt tcagacgttg tcaagatcac 2460
cctcaagtct gaggaagagg cccatttgaa aaagtcagct gatactttgt ggggtattca 2520
aaaggaacta caattctagt taattaaaca ggcccctttt cctttgtcga tatcatgtaa 2580
ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa 2640
ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta 2700
agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt 2760
aacgggcaga cggccggcca taacttcgta taatgtatgc tatacgaagt tatggcaacg 2820
gttcatcatc tcatggatct gcacatgaac aaacaccaga gtcaaacgac gttgaaattg 2880
aggctactgc gccaattgat gacaatacag acgatgataa caaaccgaag ttatctgatg 2940
tagaaaagga ttagagatgc taagagatag tgatgatatt tcataaataa tgtaattcta 3000
tatatgttaa ttaccttttt tgcgaggcat atttatggtg aaggataagt tttgaccatc 3060
aaagaaggtt aatgtggctg tggtttcagg gtccataaag cttttcaatt catctttttt 3120
ttttttgttc ttttttttga ttccggtttc tttgaaattt ttttgattcg gtaatctccg 3180
agcagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgtg 3240
gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct 3300
gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc 3360
ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg 3420
cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca 3480
aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag 3540
ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat 3600
ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag 3660
aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga 3720
agcaggcggc ggaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt 3780
catgcaaggg ctccctagct actggagaat atactaaggg tactgttgac attgcgaaga 3840
gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag 3900
gttacgattg gttgattatg acac 3924
<210> 34
<211> 332
<212> PRT
<213> 鸭嘴兽(Ornithorhynchus anatinus)
<400> 34
Met Ala Gly Val Lys Glu Gln Leu Ile Gln Asn Leu Leu Lys Glu Glu
1 5 10 15
Tyr Ala Pro Gln Asn Lys Ile Thr Val Val Gly Val Gly Ala Val Gly
20 25 30
Met Ala Cys Ala Ile Ser Ile Leu Met Lys Asp Leu Ala Asp Glu Leu
35 40 45
Ala Leu Val Asp Val Ile Glu Asp Lys Leu Lys Gly Glu Met Met Asp
50 55 60
Leu Gln His Gly Ser Leu Phe Leu Arg Thr Pro Lys Ile Val Ser Gly
65 70 75 80
Lys Asp Tyr Ser Val Thr Ala Asn Ser Lys Leu Val Ile Ile Thr Ala
85 90 95
Gly Ala Arg Gln Gln Glu Gly Glu Ser Arg Leu Asn Leu Val Gln Arg
100 105 110
Asn Val Asn Ile Phe Lys Phe Ile Ile Pro Asn Val Val Lys Tyr Ser
115 120 125
Pro Asn Cys Lys Leu Leu Val Val Ser Asn Pro Val Asp Ile Leu Thr
130 135 140
Tyr Val Ala Trp Lys Ile Ser Gly Phe Pro Lys Asn Arg Val Ile Gly
145 150 155 160
Ser Gly Cys Asn Leu Asp Ser Ala Arg Phe Arg Tyr Leu Met Gly Glu
165 170 175
Arg Leu Gly Ile His Ser Thr Ser Cys His Gly Trp Val Ile Gly Glu
180 185 190
His Gly Asp Ser Ser Val Pro Val Trp Ser Gly Val Asn Val Ala Gly
195 200 205
Val Ser Leu Lys Asn Leu His Pro Asp Leu Gly Thr Asp Ala Asp Lys
210 215 220
Glu Gln Trp Lys Asp Val His Lys Gln Val Val Asp Ser Ala Tyr Glu
225 230 235 240
Val Ile Lys Leu Lys Gly Tyr Thr Ser Trp Ala Ile Gly Leu Ser Val
245 250 255
Ala Asp Leu Ala Glu Ser Ile Val Lys Asn Leu Arg Arg Val His Pro
260 265 270
Ile Ser Thr Met Ile Lys Gly Leu Tyr Gly Ile Lys Asp Glu Val Phe
275 280 285
Leu Ser Val Pro Cys Val Leu Gly Gln Asn Gly Ile Ser Asp Val Val
290 295 300
Lys Ile Thr Leu Lys Ser Glu Glu Glu Ala His Leu Lys Lys Ser Ala
305 310 315 320
Asp Thr Leu Trp Gly Ile Gln Lys Glu Leu Gln Phe
325 330
<210> 35
<211> 4641
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和醋酸杆菌。
<400> 35
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgacaaccg ctgcagaaaa 1560
caacaagcaa ttacttgaac aactgaaaaa catagctggt caacatcacg ttctaacagg 1620
tgaagattct acttacagat tcacacacgg ttttagattt ggtgctggaa aagttctagc 1680
agtagtccaa ccagggtctt taatcgagct gtggagagta gcagacgctt gtgtcaatgc 1740
ggacaagatc atcattatgc aggccgccaa tactggtttg actggtggat caacacctga 1800
tggtaataac tacgacagag atattgtttt gatttcaacc ttgagattag acaaatctca 1860
tctcattggc aatggtaagc aagtggtatg ccttcctggt gctacactat atgaactaga 1920
gtccaagttg gccgctatcg gcagagaacc tcattcagta ataggttcat cttgcatcgg 1980
ggcaagtgtc ttgggaggcg tgtctaacaa ctctgggggt gccttggtcc aaagagggcc 2040
agcatacaca gagatggctg tttttggtca ggttggcaag gatggaaagc ttcaattggt 2100
taatcacttg ggtattaagt tgggaaacga tccagaagcc attttgcaga aactagaagc 2160
aggcacattt actgaggctg acatcgattg ggacgcgggt aagggtcatg atggcgatta 2220
cattaaccat gttagagaaa ttgatgcgga tactccagcg agatacaacg ctgatccagc 2280
acgttggtac gaagctgctg gatgtgctgg aaagttggtc gttttcgccg ttagattgga 2340
tactttccca gccaataagg acacatccgt cttttacatt ggaacaaaca atacagatga 2400
actagaaaat ctccgtagac atgtgttaac tgcatttgat gaattaccaa tttgtggtga 2460
atacattcat agagatgcct ttaacattgc agagaaatac ggtaaggata cctttgctgt 2520
tataagatac ttcggtacca agttcttacc aaaaatgttc gcaatgaaat ccagattcga 2580
tatcttcgcc aaaaagttag gcttcttgcc agaccacttg tctgatagaa taatgcaagc 2640
actaagtact cttttaccaa gacagcttcc tcaacgtatg ttagactaca gagacaagta 2700
cgaacatcac ctgatgctaa aggtcagcca taatctcgca ggcccaatga gacaatactt 2760
acaaacatac ttcggttcag ctacaggtga ctatttcgag tgtactccag aggaaggcaa 2820
gcttgcattc cttcatagat tcgctgcagc aggcgctgct gtcagataca gagcagtaca 2880
ccataaggaa gcagaggata tcgttgcttt agatgtggca ttacgtagaa acgatagaga 2940
gtggttcgaa aagctccctc aggacatcga aaacaaactg atagtgaagc tttactacgg 3000
acatttcttg tgccatgtta tgcaccaaga ttacgttgtg aaaaagggtt ataacgcaat 3060
cgccgttgaa cacgagatgt tgccaggttt ggatgccaga ggtgctagat acccagccga 3120
acacaacgtt ggtcatttgt acaaagctcc acaaaacatg ttagatcatt atcagcaact 3180
ggatccttgc aactgtatga atcctggaat cgggcaaggg accaagagaa aaaactgggt 3240
agcggaatca gtttagttaa ttaaacaggc cccttttcct ttgtcgatat catgtaatta 3300
gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga 3360
gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 3420
acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac 3480
gggcagacgg ccggccataa cttcgtataa tgtatgctat acgaagttat ggcaacggtt 3540
catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt gaaattgagg 3600
ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta tctgatgtag 3660
aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt aattctatat 3720
atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa 3780
gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat cttttttttt 3840
tttgttcttt tttttgattc cggtttcttt gaaatttttt tgattcggta atctccgagc 3900
agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtggtg 3960
ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca 4020
ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta 4080
gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt 4140
cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa 4200
tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta 4260
agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg 4320
ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat 4380
gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc 4440
aggcggcgga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat 4500
gcaagggctc cctagctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg 4560
acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt 4620
acgattggtt gattatgaca c 4641
<210> 36
<211> 571
<212> PRT
<213> 醋酸杆菌(Acetobacter aceti)
<400> 36
Met Thr Thr Ala Ala Glu Asn Asn Lys Gln Leu Leu Glu Gln Leu Lys
1 5 10 15
Asn Ile Ala Gly Gln His His Val Leu Thr Gly Glu Asp Ser Thr Tyr
20 25 30
Arg Phe Thr His Gly Phe Arg Phe Gly Ala Gly Lys Val Leu Ala Val
35 40 45
Val Gln Pro Gly Ser Leu Ile Glu Leu Trp Arg Val Ala Asp Ala Cys
50 55 60
Val Asn Ala Asp Lys Ile Ile Ile Met Gln Ala Ala Asn Thr Gly Leu
65 70 75 80
Thr Gly Gly Ser Thr Pro Asp Gly Asn Asn Tyr Asp Arg Asp Ile Val
85 90 95
Leu Ile Ser Thr Leu Arg Leu Asp Lys Ser His Leu Ile Gly Asn Gly
100 105 110
Lys Gln Val Val Cys Leu Pro Gly Ala Thr Leu Tyr Glu Leu Glu Ser
115 120 125
Lys Leu Ala Ala Ile Gly Arg Glu Pro His Ser Val Ile Gly Ser Ser
130 135 140
Cys Ile Gly Ala Ser Val Leu Gly Gly Val Ser Asn Asn Ser Gly Gly
145 150 155 160
Ala Leu Val Gln Arg Gly Pro Ala Tyr Thr Glu Met Ala Val Phe Gly
165 170 175
Gln Val Gly Lys Asp Gly Lys Leu Gln Leu Val Asn His Leu Gly Ile
180 185 190
Lys Leu Gly Asn Asp Pro Glu Ala Ile Leu Gln Lys Leu Glu Ala Gly
195 200 205
Thr Phe Thr Glu Ala Asp Ile Asp Trp Asp Ala Gly Lys Gly His Asp
210 215 220
Gly Asp Tyr Ile Asn His Val Arg Glu Ile Asp Ala Asp Thr Pro Ala
225 230 235 240
Arg Tyr Asn Ala Asp Pro Ala Arg Trp Tyr Glu Ala Ala Gly Cys Ala
245 250 255
Gly Lys Leu Val Val Phe Ala Val Arg Leu Asp Thr Phe Pro Ala Asn
260 265 270
Lys Asp Thr Ser Val Phe Tyr Ile Gly Thr Asn Asn Thr Asp Glu Leu
275 280 285
Glu Asn Leu Arg Arg His Val Leu Thr Ala Phe Asp Glu Leu Pro Ile
290 295 300
Cys Gly Glu Tyr Ile His Arg Asp Ala Phe Asn Ile Ala Glu Lys Tyr
305 310 315 320
Gly Lys Asp Thr Phe Ala Val Ile Arg Tyr Phe Gly Thr Lys Phe Leu
325 330 335
Pro Lys Met Phe Ala Met Lys Ser Arg Phe Asp Ile Phe Ala Lys Lys
340 345 350
Leu Gly Phe Leu Pro Asp His Leu Ser Asp Arg Ile Met Gln Ala Leu
355 360 365
Ser Thr Leu Leu Pro Arg Gln Leu Pro Gln Arg Met Leu Asp Tyr Arg
370 375 380
Asp Lys Tyr Glu His His Leu Met Leu Lys Val Ser His Asn Leu Ala
385 390 395 400
Gly Pro Met Arg Gln Tyr Leu Gln Thr Tyr Phe Gly Ser Ala Thr Gly
405 410 415
Asp Tyr Phe Glu Cys Thr Pro Glu Glu Gly Lys Leu Ala Phe Leu His
420 425 430
Arg Phe Ala Ala Ala Gly Ala Ala Val Arg Tyr Arg Ala Val His His
435 440 445
Lys Glu Ala Glu Asp Ile Val Ala Leu Asp Val Ala Leu Arg Arg Asn
450 455 460
Asp Arg Glu Trp Phe Glu Lys Leu Pro Gln Asp Ile Glu Asn Lys Leu
465 470 475 480
Ile Val Lys Leu Tyr Tyr Gly His Phe Leu Cys His Val Met His Gln
485 490 495
Asp Tyr Val Val Lys Lys Gly Tyr Asn Ala Ile Ala Val Glu His Glu
500 505 510
Met Leu Pro Gly Leu Asp Ala Arg Gly Ala Arg Tyr Pro Ala Glu His
515 520 525
Asn Val Gly His Leu Tyr Lys Ala Pro Gln Asn Met Leu Asp His Tyr
530 535 540
Gln Gln Leu Asp Pro Cys Asn Cys Met Asn Pro Gly Ile Gly Gln Gly
545 550 555 560
Thr Lys Arg Lys Asn Trp Val Ala Glu Ser Val
565 570
<210> 37
<211> 4360
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和氧化葡糖杆菌。
<400> 37
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgccagaac cagttatgac 1560
agcctcctct gcatctgctc cagatagact acaagccgta ttgaaagcct tacagccagt 1620
tatgggagaa cgtatatcta ctgctccttc cgttagagag gagcatagtc atggtgaggc 1680
tatgaacgct tccaaccttc ctgaagcagt agtttttgct gaatctactc aagatgttgc 1740
aaccgttctc agacactgcc acgaatggcg tgtcccagtc gttgcattcg gtgcaggaac 1800
ttcagtagag ggtcacgtgg tgccacctga acaagcaata agtcttgatc tgagtagaat 1860
gactgggatt gtggacttga acgcagaaga tttggattgt agagtccaag ctggtataac 1920
tagacaaacc cttaacgtcg agattagaga cactggccta ttctttccag tcgatcctgg 1980
tggtgaggca acaatcggtg gaatgtgtgc cacaagagct tcaggtacag cagcagttag 2040
atacggtaca atgaaggaaa atgttttagg actcacagtg gttttagcca ctggtgaaat 2100
cattagaaca ggaggaagag tcagaaagtc tagtacagga tatgatttaa cctctttgtt 2160
cgtcggctct gaaggcacac tgggaattat caccgaggtt caattaagac tacatggccg 2220
tcctgattca gtttcagctg caatttgtca atttgagtca ttgcatgacg ccattcaaac 2280
agctatggaa atcatccaat gcggtattcc aatcacaaga gtagaattga tggacagcgt 2340
tcaaatggca gcctcaatcc agtactctgg cctcaacgaa taccaacctc taacaacttt 2400
attcttcgaa ttcacaggct ctcctgctgc cgtcagagaa caggtggaaa ctacagaggc 2460
tatagcttcc ggtaataacg gtctaggttt cgcgtgggct gaatccccag aagatagaac 2520
tagattgtgg aaggccagac atgatgctta ctgggcggct aaagcgattg ttcctgatgc 2580
aagagttatt tctacagatt gtatcgttcc aatttcaaga ttaggtgaac ttattgaagg 2640
tgtccataga gatattgaag catcaggtct gagagcccca cttttgggcc atgtgggtga 2700
tgggaacttt cacacattga tcataaccga cgacactcca gagggtcatc agcaagcttt 2760
ggatttggat agaaagatcg tggctcgtgc acttagccta aatgggtcat gctctgggga 2820
acacggcgta ggtatgggga agttagagtt cttagagaca gaacacgggc ctggttcatt 2880
gtctgttatg agagcattga aaaacaccat ggacccacat catatcttaa atccaggaaa 2940
gctgttgcca ccaggcgcgg tatacactgg ttagttaatt aaacaggccc cttttccttt 3000
gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc cacatccgct 3060
ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 3120
gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 3180
cgcgtgtacg catgtaacgg gcagacggcc ggccataact tcgtataatg tatgctatac 3240
gaagttatgg caacggttca tcatctcatg gatctgcaca tgaacaaaca ccagagtcaa 3300
acgacgttga aattgaggct actgcgccaa ttgatgacaa tacagacgat gataacaaac 3360
cgaagttatc tgatgtagaa aaggattaga gatgctaaga gatagtgatg atatttcata 3420
aataatgtaa ttctatatat gttaattacc ttttttgcga ggcatattta tggtgaagga 3480
taagttttga ccatcaaaga aggttaatgt ggctgtggtt tcagggtcca taaagctttt 3540
caattcatct tttttttttt tgttcttttt tttgattccg gtttctttga aatttttttg 3600
attcggtaat ctccgagcag aaggaagaac gaaggaagga gcacagactt agattggtat 3660
atatacgcat atgtggtgtt gaagaaacat gaaattgccc agtattctta acccaactgc 3720
acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac 3780
gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc 3840
aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg 3900
aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt 3960
ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct 4020
tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg 4080
tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta 4140
ttgttagcgg tttgaagcag gcggcggaag aagtaacaaa ggaacctaga ggccttttga 4200
tgttagcaga attgtcatgc aagggctccc tagctactgg agaatatact aagggtactg 4260
ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg 4320
gtggaagaga tgaaggttac gattggttga ttatgacacg 4360
<210> 38
<211> 477
<212> PRT
<213> 氧化葡糖杆菌(Gluconobacter oxydans)
<400> 38
Met Pro Glu Pro Val Met Thr Ala Ser Ser Ala Ser Ala Pro Asp Arg
1 5 10 15
Leu Gln Ala Val Leu Lys Ala Leu Gln Pro Val Met Gly Glu Arg Ile
20 25 30
Ser Thr Ala Pro Ser Val Arg Glu Glu His Ser His Gly Glu Ala Met
35 40 45
Asn Ala Ser Asn Leu Pro Glu Ala Val Val Phe Ala Glu Ser Thr Gln
50 55 60
Asp Val Ala Thr Val Leu Arg His Cys His Glu Trp Arg Val Pro Val
65 70 75 80
Val Ala Phe Gly Ala Gly Thr Ser Val Glu Gly His Val Val Pro Pro
85 90 95
Glu Gln Ala Ile Ser Leu Asp Leu Ser Arg Met Thr Gly Ile Val Asp
100 105 110
Leu Asn Ala Glu Asp Leu Asp Cys Arg Val Gln Ala Gly Ile Thr Arg
115 120 125
Gln Thr Leu Asn Val Glu Ile Arg Asp Thr Gly Leu Phe Phe Pro Val
130 135 140
Asp Pro Gly Gly Glu Ala Thr Ile Gly Gly Met Cys Ala Thr Arg Ala
145 150 155 160
Ser Gly Thr Ala Ala Val Arg Tyr Gly Thr Met Lys Glu Asn Val Leu
165 170 175
Gly Leu Thr Val Val Leu Ala Thr Gly Glu Ile Ile Arg Thr Gly Gly
180 185 190
Arg Val Arg Lys Ser Ser Thr Gly Tyr Asp Leu Thr Ser Leu Phe Val
195 200 205
Gly Ser Glu Gly Thr Leu Gly Ile Ile Thr Glu Val Gln Leu Arg Leu
210 215 220
His Gly Arg Pro Asp Ser Val Ser Ala Ala Ile Cys Gln Phe Glu Ser
225 230 235 240
Leu His Asp Ala Ile Gln Thr Ala Met Glu Ile Ile Gln Cys Gly Ile
245 250 255
Pro Ile Thr Arg Val Glu Leu Met Asp Ser Val Gln Met Ala Ala Ser
260 265 270
Ile Gln Tyr Ser Gly Leu Asn Glu Tyr Gln Pro Leu Thr Thr Leu Phe
275 280 285
Phe Glu Phe Thr Gly Ser Pro Ala Ala Val Arg Glu Gln Val Glu Thr
290 295 300
Thr Glu Ala Ile Ala Ser Gly Asn Asn Gly Leu Gly Phe Ala Trp Ala
305 310 315 320
Glu Ser Pro Glu Asp Arg Thr Arg Leu Trp Lys Ala Arg His Asp Ala
325 330 335
Tyr Trp Ala Ala Lys Ala Ile Val Pro Asp Ala Arg Val Ile Ser Thr
340 345 350
Asp Cys Ile Val Pro Ile Ser Arg Leu Gly Glu Leu Ile Glu Gly Val
355 360 365
His Arg Asp Ile Glu Ala Ser Gly Leu Arg Ala Pro Leu Leu Gly His
370 375 380
Val Gly Asp Gly Asn Phe His Thr Leu Ile Ile Thr Asp Asp Thr Pro
385 390 395 400
Glu Gly His Gln Gln Ala Leu Asp Leu Asp Arg Lys Ile Val Ala Arg
405 410 415
Ala Leu Ser Leu Asn Gly Ser Cys Ser Gly Glu His Gly Val Gly Met
420 425 430
Gly Lys Leu Glu Phe Leu Glu Thr Glu His Gly Pro Gly Ser Leu Ser
435 440 445
Val Met Arg Ala Leu Lys Asn Thr Met Asp Pro His His Ile Leu Asn
450 455 460
Pro Gly Lys Leu Leu Pro Pro Gly Ala Val Tyr Thr Gly
465 470 475
<210> 39
<211> 4689
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 39
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgttgtgga agcgtacttg 1560
cacaaggcta ataaagccta ttgcacaacc tagaggaagg ctggtgagaa gatcatgcta 1620
cagatacgcc tcaacaggca caggcagcac cgacagcagc agtcagtggt taaaatactc 1680
tgtcatcgcc tcttcagcta ctctattcgg ttatttgttc gctaagaacc tctattctag 1740
ggagactaag gaggacctga tagagaaact ggaaatggtc aaaaagatcg acccagtaaa 1800
ttctacgtta aagctgtcct cattggactc accagactat ttgcacgacc cggccaagat 1860
cgataaggtt gttgaggacc tgaagcaggt gctgggaaac aagcctgaaa actactctga 1920
tgcgaaatcc gatttggacg cccattcaga tacctacttc aacacgcatc acccctctct 1980
cgagcaaaga cctaggatta tattattccc tcatactacc gaagaagttt ccaaaatttt 2040
gaaaatatgt cacgataaca acatgccagt tgtacccttc tcgggcggaa cgtccttgga 2100
ggggcacttc ctgcctacaa gaattggaga taccataacc gtagacctgt ccaagtttat 2160
gaataacgtc gtaaaatttg acaagctgga cctggacatc accgtgcagg ccggtctacc 2220
ctgggaggat ttgaatgact atttgagcga ccacggtttg atgtttggct gtgaccctgg 2280
tccaggtgca cagattggtg gttgcattgc taattcttgt tcaggaacca acgcctaccg 2340
ttacggtacc atgaaggaga atattataaa catgactata gtgttgccgg acgggaccat 2400
cgtcaagacg aagaaaagac ccagaaagtc gagcgctggc tataacttaa atgggttatt 2460
tgtgggaagt gaaggtacct taggtattgt tactgaagct actgtcaagt gtcatgtcaa 2520
gcccaaagct gaaactgttg cggtggtatc ctttgatact atcaaggatg cggccgcatg 2580
tgcttctaat ctgactcaga gtggtattca tttgaacgcc atggagttac tggatgaaaa 2640
tatgatgaag ttgatcaacg catctgaatc cacggacaga tgtgattggg tagagaaacc 2700
aactatgttt ttcaagattg gtgggagatc tcccaacatt gtcaatgctc ttgtggatga 2760
agttaaggct gtcgcccagt taaatcactg caacagtttt cagtttgcta aagatgatga 2820
cgaaaaattg gaattatggg aagctagaaa ggtcgcgcta tggtctgtgc tagacgctga 2880
taagagcaaa gacaaatctg ctaaaatttg gacaactgat gtagctgttc ctgtgtcgca 2940
gttcgacaag gttattcacg aaactaaaaa ggacatgcaa gctagtaagc tgatcaacgc 3000
cattgttggt catgcaggtg atggtaactt ccatgcattc atcgtctaca gaacccctga 3060
agaacacgaa acctgtagcc aacttgttga cagaatggtc aagagagcac tggacgcaga 3120
aggcacttgc acgggtgaac acggtgttgg tattggtaaa agagagtact tgctcgaaga 3180
attaggtgaa gcacccgtcg atttgatgag aaagattaag ctagctattg acccaaagag 3240
aatcatgaac ccggacaaaa tctttaaaac tgatccaaac gagcccgcta atgattacag 3300
gtgattaatt aaacaggccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc 3360
ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct 3420
gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata 3480
tttcaaattt ttcttttttt tctgtacaaa cgcgtgtacg catgtaacgg gcagacggcc 3540
ggccataact tcgtataatg tatgctatac gaagttatgg caacggttca tcatctcatg 3600
gatctgcaca tgaacaaaca ccagagtcaa acgacgttga aattgaggct actgcgccaa 3660
ttgatgacaa tacagacgat gataacaaac cgaagttatc tgatgtagaa aaggattaga 3720
gatgctaaga gatagtgatg atatttcata aataatgtaa ttctatatat gttaattacc 3780
ttttttgcga ggcatattta tggtgaagga taagttttga ccatcaaaga aggttaatgt 3840
ggctgtggtt tcagggtcca taaagctttt caattcatct tttttttttt tgttcttttt 3900
tttgattccg gtttctttga aatttttttg attcggtaat ctccgagcag aaggaagaac 3960
gaaggaagga gcacagactt agattggtat atatacgcat atgtggtgtt gaagaaacat 4020
gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat 4080
aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg 4140
ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc 4200
gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa 4260
aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg 4320
cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta 4380
atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta 4440
cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcggaag 4500
aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc 4560
tagctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg 4620
ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga 4680
ttatgacac 4689
<210> 40
<211> 587
<212> PRT
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 40
Met Leu Trp Lys Arg Thr Cys Thr Arg Leu Ile Lys Pro Ile Ala Gln
1 5 10 15
Pro Arg Gly Arg Leu Val Arg Arg Ser Cys Tyr Arg Tyr Ala Ser Thr
20 25 30
Gly Thr Gly Ser Thr Asp Ser Ser Ser Gln Trp Leu Lys Tyr Ser Val
35 40 45
Ile Ala Ser Ser Ala Thr Leu Phe Gly Tyr Leu Phe Ala Lys Asn Leu
50 55 60
Tyr Ser Arg Glu Thr Lys Glu Asp Leu Ile Glu Lys Leu Glu Met Val
65 70 75 80
Lys Lys Ile Asp Pro Val Asn Ser Thr Leu Lys Leu Ser Ser Leu Asp
85 90 95
Ser Pro Asp Tyr Leu His Asp Pro Ala Lys Ile Asp Lys Val Val Glu
100 105 110
Asp Leu Lys Gln Val Leu Gly Asn Lys Pro Glu Asn Tyr Ser Asp Ala
115 120 125
Lys Ser Asp Leu Asp Ala His Ser Asp Thr Tyr Phe Asn Thr His His
130 135 140
Pro Ser Leu Glu Gln Arg Pro Arg Ile Ile Leu Phe Pro His Thr Thr
145 150 155 160
Glu Glu Val Ser Lys Ile Leu Lys Ile Cys His Asp Asn Asn Met Pro
165 170 175
Val Val Pro Phe Ser Gly Gly Thr Ser Leu Glu Gly His Phe Leu Pro
180 185 190
Thr Arg Ile Gly Asp Thr Ile Thr Val Asp Leu Ser Lys Phe Met Asn
195 200 205
Asn Val Val Lys Phe Asp Lys Leu Asp Leu Asp Ile Thr Val Gln Ala
210 215 220
Gly Leu Pro Trp Glu Asp Leu Asn Asp Tyr Leu Ser Asp His Gly Leu
225 230 235 240
Met Phe Gly Cys Asp Pro Gly Pro Gly Ala Gln Ile Gly Gly Cys Ile
245 250 255
Ala Asn Ser Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Lys
260 265 270
Glu Asn Ile Ile Asn Met Thr Ile Val Leu Pro Asp Gly Thr Ile Val
275 280 285
Lys Thr Lys Lys Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asn Leu Asn
290 295 300
Gly Leu Phe Val Gly Ser Glu Gly Thr Leu Gly Ile Val Thr Glu Ala
305 310 315 320
Thr Val Lys Cys His Val Lys Pro Lys Ala Glu Thr Val Ala Val Val
325 330 335
Ser Phe Asp Thr Ile Lys Asp Ala Ala Ala Cys Ala Ser Asn Leu Thr
340 345 350
Gln Ser Gly Ile His Leu Asn Ala Met Glu Leu Leu Asp Glu Asn Met
355 360 365
Met Lys Leu Ile Asn Ala Ser Glu Ser Thr Asp Arg Cys Asp Trp Val
370 375 380
Glu Lys Pro Thr Met Phe Phe Lys Ile Gly Gly Arg Ser Pro Asn Ile
385 390 395 400
Val Asn Ala Leu Val Asp Glu Val Lys Ala Val Ala Gln Leu Asn His
405 410 415
Cys Asn Ser Phe Gln Phe Ala Lys Asp Asp Asp Glu Lys Leu Glu Leu
420 425 430
Trp Glu Ala Arg Lys Val Ala Leu Trp Ser Val Leu Asp Ala Asp Lys
435 440 445
Ser Lys Asp Lys Ser Ala Lys Ile Trp Thr Thr Asp Val Ala Val Pro
450 455 460
Val Ser Gln Phe Asp Lys Val Ile His Glu Thr Lys Lys Asp Met Gln
465 470 475 480
Ala Ser Lys Leu Ile Asn Ala Ile Val Gly His Ala Gly Asp Gly Asn
485 490 495
Phe His Ala Phe Ile Val Tyr Arg Thr Pro Glu Glu His Glu Thr Cys
500 505 510
Ser Gln Leu Val Asp Arg Met Val Lys Arg Ala Leu Asp Ala Glu Gly
515 520 525
Thr Cys Thr Gly Glu His Gly Val Gly Ile Gly Lys Arg Glu Tyr Leu
530 535 540
Leu Glu Glu Leu Gly Glu Ala Pro Val Asp Leu Met Arg Lys Ile Lys
545 550 555 560
Leu Ala Ile Asp Pro Lys Arg Ile Met Asn Pro Asp Lys Ile Phe Lys
565 570 575
Thr Asp Pro Asn Glu Pro Ala Asn Asp Tyr Arg
580 585
<210> 41
<211> 4527
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和东方伊萨酵母。
<400> 41
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgtcaagat tgtttaaggt 1560
gctcaaagca actcaattca aatatgtgtc ttcatttctt cttggatcga caatctccgg 1620
cggagtggtg tattacaatt ataagaaatc attgaatttc agctttcctc caacatccac 1680
taccccaatt acagctgcac cacctttaca atatggggat gtatccaaag ctatatctga 1740
gatcacatca aaattgggac cttcaaagat aactttctca gagagcgaaa ttttacacca 1800
ctctgacagt tcattctcaa cggatcatgc aaaaccacat gaacgccccc atgctgttgt 1860
ttatcctgaa tccactgaag atgtttctga agtcttgaaa atttgtcata ctttgaaagt 1920
ccctgtcgtg ccattcaccg gtgggacctc gcttgaaggt cactttatat ccacaagaaa 1980
tggtatctct gttgatttgt cgaggatgaa caaagtcctt gcaattcatc cagaagattt 2040
ggatgcagtt gttcagcctg ctgttggctg ggaagagtta agagatacgt tatccgacta 2100
taacttgttg tttggccctg atcctggccc aggtgcttgt attggtggta tggttgcgac 2160
ttcctgttcc ggtacaaatg ctgcaaggta tggtacaatg agagaaaatg ttgtttctgt 2220
taaagttgtg cttccagatg ggactattgt gaaaactaaa agaagaccaa ggaaatcaag 2280
tgctggttat aatctaacta atttattcat tggtagtgaa ggtaccttgg gtattgtgac 2340
cgaagttact ttgaaactga acgtcaagcc aaaatatgag agtgttgcat tggtctcgtt 2400
tgataagtta aaagatgctg ctggatcagt tactagcttt gtccaagaag gtctacaact 2460
aaacgcgatt gaattacttg atgataagat ggtacatttt gttaatgagt ccggtgaaac 2520
cgatttgaaa tacgatgagc ttccaacttt acttttaaag attggcggct catctcctga 2580
gtcggtgaat ttgctaatca aaactgtcga gaggattgtc aatgctaaca atgctaagac 2640
ttttaagttt gcctccaacg aagaggagaa gtatcaattg tggaatgccc gtaaaactgc 2700
actttggtca actatcgaat atggccgcag aaatatagac aaggatatcc aggtttggac 2760
gactgacgtt gcagttccaa tgtcaaaatt tgtggaagca gtcgaagaaa cccaaaagga 2820
aatcgaagaa tccgatttgc ttgcatctat tgttggccac gcaggtgatg gaaactatca 2880
taccgttatt ttattcaagg aagaacagcg tgccttggct gcaaaattgg tctcaaatat 2940
ggttgatcgt gctctagcct ttgatggtac cgttagtggt gagcatggga ttggtgttgg 3000
caagaaggag tttttgattg atgaagttgg tcaagaatct gtggacttga tgaggaaaat 3060
taaattctct attgaccctc ataaaatttt gaaccctgat aaggtcttct cgatcgatcc 3120
tgtaaacgat agatcgatat aattaattaa acaggcccct tttcctttgt cgatatcatg 3180
taattagtta tgtcacgctt acattcacgc cctcctccca catccgctct aaccgaaaag 3240
gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta 3300
ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacaaacg cgtgtacgca 3360
tgtaacgggc agacggccgg ccataacttc gtataatgta tgctatacga agttatggca 3420
acggttcatc atctcatgga tctgcacatg aacaaacacc agagtcaaac gacgttgaaa 3480
ttgaggctac tgcgccaatt gatgacaata cagacgatga taacaaaccg aagttatctg 3540
atgtagaaaa ggattagaga tgctaagaga tagtgatgat atttcataaa taatgtaatt 3600
ctatatatgt taattacctt ttttgcgagg catatttatg gtgaaggata agttttgacc 3660
atcaaagaag gttaatgtgg ctgtggtttc agggtccata aagcttttca attcatcttt 3720
tttttttttg ttcttttttt tgattccggt ttctttgaaa tttttttgat tcggtaatct 3780
ccgagcagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat 3840
gtggtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa 3900
cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc 3960
atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt 4020
gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc 4080
ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca 4140
cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa 4200
aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag 4260
cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt 4320
tgaagcaggc ggcggaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat 4380
tgtcatgcaa gggctcccta gctactggag aatatactaa gggtactgtt gacattgcga 4440
agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg 4500
aaggttacga ttggttgatt atgacac 4527
<210> 42
<211> 533
<212> PRT
<213> 东方伊萨酵母(Issatchenkia orientalis)
<400> 42
Met Ser Arg Leu Phe Lys Val Leu Lys Ala Thr Gln Phe Lys Tyr Val
1 5 10 15
Ser Ser Phe Leu Leu Gly Ser Thr Ile Ser Gly Gly Val Val Tyr Tyr
20 25 30
Asn Tyr Lys Lys Ser Leu Asn Phe Ser Phe Pro Pro Thr Ser Thr Thr
35 40 45
Pro Ile Thr Ala Ala Pro Pro Leu Gln Tyr Gly Asp Val Ser Lys Ala
50 55 60
Ile Ser Glu Ile Thr Ser Lys Leu Gly Pro Ser Lys Ile Thr Phe Ser
65 70 75 80
Glu Ser Glu Ile Leu His His Ser Asp Ser Ser Phe Ser Thr Asp His
85 90 95
Ala Lys Pro His Glu Arg Pro His Ala Val Val Tyr Pro Glu Ser Thr
100 105 110
Glu Asp Val Ser Glu Val Leu Lys Ile Cys His Thr Leu Lys Val Pro
115 120 125
Val Val Pro Phe Thr Gly Gly Thr Ser Leu Glu Gly His Phe Ile Ser
130 135 140
Thr Arg Asn Gly Ile Ser Val Asp Leu Ser Arg Met Asn Lys Val Leu
145 150 155 160
Ala Ile His Pro Glu Asp Leu Asp Ala Val Val Gln Pro Ala Val Gly
165 170 175
Trp Glu Glu Leu Arg Asp Thr Leu Ser Asp Tyr Asn Leu Leu Phe Gly
180 185 190
Pro Asp Pro Gly Pro Gly Ala Cys Ile Gly Gly Met Val Ala Thr Ser
195 200 205
Cys Ser Gly Thr Asn Ala Ala Arg Tyr Gly Thr Met Arg Glu Asn Val
210 215 220
Val Ser Val Lys Val Val Leu Pro Asp Gly Thr Ile Val Lys Thr Lys
225 230 235 240
Arg Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asn Leu Thr Asn Leu Phe
245 250 255
Ile Gly Ser Glu Gly Thr Leu Gly Ile Val Thr Glu Val Thr Leu Lys
260 265 270
Leu Asn Val Lys Pro Lys Tyr Glu Ser Val Ala Leu Val Ser Phe Asp
275 280 285
Lys Leu Lys Asp Ala Ala Gly Ser Val Thr Ser Phe Val Gln Glu Gly
290 295 300
Leu Gln Leu Asn Ala Ile Glu Leu Leu Asp Asp Lys Met Val His Phe
305 310 315 320
Val Asn Glu Ser Gly Glu Thr Asp Leu Lys Tyr Asp Glu Leu Pro Thr
325 330 335
Leu Leu Leu Lys Ile Gly Gly Ser Ser Pro Glu Ser Val Asn Leu Leu
340 345 350
Ile Lys Thr Val Glu Arg Ile Val Asn Ala Asn Asn Ala Lys Thr Phe
355 360 365
Lys Phe Ala Ser Asn Glu Glu Glu Lys Tyr Gln Leu Trp Asn Ala Arg
370 375 380
Lys Thr Ala Leu Trp Ser Thr Ile Glu Tyr Gly Arg Arg Asn Ile Asp
385 390 395 400
Lys Asp Ile Gln Val Trp Thr Thr Asp Val Ala Val Pro Met Ser Lys
405 410 415
Phe Val Glu Ala Val Glu Glu Thr Gln Lys Glu Ile Glu Glu Ser Asp
420 425 430
Leu Leu Ala Ser Ile Val Gly His Ala Gly Asp Gly Asn Tyr His Thr
435 440 445
Val Ile Leu Phe Lys Glu Glu Gln Arg Ala Leu Ala Ala Lys Leu Val
450 455 460
Ser Asn Met Val Asp Arg Ala Leu Ala Phe Asp Gly Thr Val Ser Gly
465 470 475 480
Glu His Gly Ile Gly Val Gly Lys Lys Glu Phe Leu Ile Asp Glu Val
485 490 495
Gly Gln Glu Ser Val Asp Leu Met Arg Lys Ile Lys Phe Ser Ile Asp
500 505 510
Pro His Lys Ile Leu Asn Pro Asp Lys Val Phe Ser Ile Asp Pro Val
515 520 525
Asn Asp Arg Ser Ile
530
<210> 43
<211> 4506
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和东方伊萨酵母。
<400> 43
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgtttgcat tagcttctat 1560
ttcacgtctg ggacgtgcaa gcgcaagcgc acgtgcacgt gcatccgcag ctctgtctat 1620
ccgtggattt gcgttgactg ctgaaaaata tccttcagtt aagcgtggag actacgccaa 1680
actaaccgat gaagatctcg gtaaattcca ttcgattttg aacccaaacc agattattac 1740
ggatgctgca gatttggctg catttaatca ggattggatg aagaaatacg ctggtcaatc 1800
gcaacttgta ttgaaaccaa agacaactca acaggtttct caaattttga agtattgtaa 1860
cgagaggaaa cttgccgttg ttccacaagg tggtaatacc ggattggttg gaggttctgt 1920
tccagttttc gacgaaatcg ttatttctat ggccggcatg aaccaaatta gatcctttga 1980
tgaaaattct ggaacattga aagtggacgc tggtgtcata cttgaaaatg cggacaacta 2040
tctggcagag aggggttata tattccccct tgatttggga gctaaaggtt catgttttgt 2100
tggtggtaac gttgcaacca atgctggtgg gttgagatta ttaagattcg gctcattaca 2160
tggctcagtg ttggggttgg aagttgtctt agctgatggt accattgtgg actccatgca 2220
ttctttaaga aaagacaata ccgggtttga tttgaagcag ctattcattg gttccgaagg 2280
taccttggga attattactg gtgtatcgat cttatgtccc gcaagaccaa aggccgtcaa 2340
tatcgcattt cttggcttgg agagctacga gcatgtgatc cagtgcttca aggaagcacg 2400
ttctgatttg ggcgaaatct taagtgcctt tgagtttatg gatcgtgatt cccagttggt 2460
tgcctccaaa ttcttgaaaa cgacccatcc attgtgtcaa gaggacgaag ttgctgaagc 2520
ttctgttccg gaatatccat tctatgtatt gctggagaca tcaggatcaa acaaagacca 2580
tgatgatgag aaactagaaa agtttttaga aaaagttatg gaaaacgaag ttgtagttga 2640
cggtacagtg tcgcaggatg aagctcaact caagactcta tggacttgga gagaaggtat 2700
cgctgaagca tcacagcaat ttggtggtgt ttataagtac gatgtctcat taccattgga 2760
ttccttgtac aaactagtcg aagagaccca ggagagacta aagagtttca gtttggcatc 2820
gaccgaggat gaatcaaaac ctgtatatga ggctattggt tatggtcata tcggtgacgg 2880
caatttacat ttgaacgttg ccgttagaga gtataacaaa gaggttgaaa aggcacttga 2940
accttttgta tatgagttta tcgagaaaca caatggttcc atcagtgccg aacatggtct 3000
tggattccaa aagaagaatt acattggtta ttctaagagt gaggttgaaa tcaaaatgat 3060
gaaggagttg aagaaccact atgatccgaa cggtattttg aacccttata agtatatctg 3120
attaattaaa caggcccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta 3180
cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag acaacctgaa 3240
gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt 3300
caaatttttc ttttttttct gtacaaacgc gtgtacgcat gtaacgggca gacggccggc 3360
cataacttcg tataatgtat gctatacgaa gttatggcaa cggttcatca tctcatggat 3420
ctgcacatga acaaacacca gagtcaaacg acgttgaaat tgaggctact gcgccaattg 3480
atgacaatac agacgatgat aacaaaccga agttatctga tgtagaaaag gattagagat 3540
gctaagagat agtgatgata tttcataaat aatgtaattc tatatatgtt aattaccttt 3600
tttgcgaggc atatttatgg tgaaggataa gttttgacca tcaaagaagg ttaatgtggc 3660
tgtggtttca gggtccataa agcttttcaa ttcatctttt ttttttttgt tctttttttt 3720
gattccggtt tctttgaaat ttttttgatt cggtaatctc cgagcagaag gaagaacgaa 3780
ggaaggagca cagacttaga ttggtatata tacgcatatg tggtgttgaa gaaacatgaa 3840
attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa 3900
tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca 3960
agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta 4020
ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa 4080
cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat 4140
tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata 4200
cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga 4260
atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcggaagaag 4320
taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctag 4380
ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta 4440
tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta 4500
tgacac 4506
<210> 44
<211> 526
<212> PRT
<213> 东方伊萨酵母(Issatchenkia orientalis)
<400> 44
Met Phe Ala Leu Ala Ser Ile Ser Arg Leu Gly Arg Ala Ser Ala Ser
1 5 10 15
Ala Arg Ala Arg Ala Ser Ala Ala Leu Ser Ile Arg Gly Phe Ala Leu
20 25 30
Thr Ala Glu Lys Tyr Pro Ser Val Lys Arg Gly Asp Tyr Ala Lys Leu
35 40 45
Thr Asp Glu Asp Leu Gly Lys Phe His Ser Ile Leu Asn Pro Asn Gln
50 55 60
Ile Ile Thr Asp Ala Ala Asp Leu Ala Ala Phe Asn Gln Asp Trp Met
65 70 75 80
Lys Lys Tyr Ala Gly Gln Ser Gln Leu Val Leu Lys Pro Lys Thr Thr
85 90 95
Gln Gln Val Ser Gln Ile Leu Lys Tyr Cys Asn Glu Arg Lys Leu Ala
100 105 110
Val Val Pro Gln Gly Gly Asn Thr Gly Leu Val Gly Gly Ser Val Pro
115 120 125
Val Phe Asp Glu Ile Val Ile Ser Met Ala Gly Met Asn Gln Ile Arg
130 135 140
Ser Phe Asp Glu Asn Ser Gly Thr Leu Lys Val Asp Ala Gly Val Ile
145 150 155 160
Leu Glu Asn Ala Asp Asn Tyr Leu Ala Glu Arg Gly Tyr Ile Phe Pro
165 170 175
Leu Asp Leu Gly Ala Lys Gly Ser Cys Phe Val Gly Gly Asn Val Ala
180 185 190
Thr Asn Ala Gly Gly Leu Arg Leu Leu Arg Phe Gly Ser Leu His Gly
195 200 205
Ser Val Leu Gly Leu Glu Val Val Leu Ala Asp Gly Thr Ile Val Asp
210 215 220
Ser Met His Ser Leu Arg Lys Asp Asn Thr Gly Phe Asp Leu Lys Gln
225 230 235 240
Leu Phe Ile Gly Ser Glu Gly Thr Leu Gly Ile Ile Thr Gly Val Ser
245 250 255
Ile Leu Cys Pro Ala Arg Pro Lys Ala Val Asn Ile Ala Phe Leu Gly
260 265 270
Leu Glu Ser Tyr Glu His Val Ile Gln Cys Phe Lys Glu Ala Arg Ser
275 280 285
Asp Leu Gly Glu Ile Leu Ser Ala Phe Glu Phe Met Asp Arg Asp Ser
290 295 300
Gln Leu Val Ala Ser Lys Phe Leu Lys Thr Thr His Pro Leu Cys Gln
305 310 315 320
Glu Asp Glu Val Ala Glu Ala Ser Val Pro Glu Tyr Pro Phe Tyr Val
325 330 335
Leu Leu Glu Thr Ser Gly Ser Asn Lys Asp His Asp Asp Glu Lys Leu
340 345 350
Glu Lys Phe Leu Glu Lys Val Met Glu Asn Glu Val Val Val Asp Gly
355 360 365
Thr Val Ser Gln Asp Glu Ala Gln Leu Lys Thr Leu Trp Thr Trp Arg
370 375 380
Glu Gly Ile Ala Glu Ala Ser Gln Gln Phe Gly Gly Val Tyr Lys Tyr
385 390 395 400
Asp Val Ser Leu Pro Leu Asp Ser Leu Tyr Lys Leu Val Glu Glu Thr
405 410 415
Gln Glu Arg Leu Lys Ser Phe Ser Leu Ala Ser Thr Glu Asp Glu Ser
420 425 430
Lys Pro Val Tyr Glu Ala Ile Gly Tyr Gly His Ile Gly Asp Gly Asn
435 440 445
Leu His Leu Asn Val Ala Val Arg Glu Tyr Asn Lys Glu Val Glu Lys
450 455 460
Ala Leu Glu Pro Phe Val Tyr Glu Phe Ile Glu Lys His Asn Gly Ser
465 470 475 480
Ile Ser Ala Glu His Gly Leu Gly Phe Gln Lys Lys Asn Tyr Ile Gly
485 490 495
Tyr Ser Lys Ser Glu Val Glu Ile Lys Met Met Lys Glu Leu Lys Asn
500 505 510
His Tyr Asp Pro Asn Gly Ile Leu Asn Pro Tyr Lys Tyr Ile
515 520 525
<210> 45
<211> 4629
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和东方伊萨酵母。
<400> 45
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgataatgt tgccgttgtc 1560
acgtgccgcc gtgcacctat cgagaactag acattcctct ctagtctcac taagacatgc 1620
acttgcaatt cctcaaaggg tgcaatactc tcaccaccgt cactactccg ccaataccga 1680
aaagactgca aaaactggtt ctgaaaagga tcccaagtct caagctagaa aggatttagg 1740
aaagtccttc ttgtctacaa cagccatact attcattggt gccttggtgg ggacatcatg 1800
ggcctcattc cagctatgca agaatccgcc tgagttttta ttcccacata catcgacatt 1860
cccgctaaag gatgccatgc cgccactata tggggattcc cagaaggtca ttgacgagtt 1920
gaaaccgctt ctgcgagaag accaaatcac aatttcaaag agtgaactcg atacacattc 1980
cgatgcttat ttctccacag accacccgag cgatgaggaa agaccagatg ctattgtcta 2040
tcctgaatcg accgaacagg tttcccagat cctcaaaata gcccataacc atcacgttcc 2100
aattgtcccc tacactggtg gcacctcatt ggagggacat tatattccaa ctaggaaggg 2160
tatctgtgtt gatttgtcta ggttaaacaa agttttggct ttacataagg aagatttgga 2220
tattgttgtc caaccagcag ttggttggga agatctggat gagtttttaa gacctcaagg 2280
tcttttattt ggacctgatc caggacctgg cgcttgtatt ggtggtatgt gtggtacatc 2340
ttgttctgga actaatgctg cgagatatgg tacaatgaga gaaaatgtgg tctcattaac 2400
tgtggtcttg gccgatggta ctttgggtat tgtcacagaa gcaactttga aactacacgt 2460
tgctccaaaa tttgaaaacg tggcagttgt taatttccct acattgaggg atgccgccaa 2520
tgcagttgcc aaaatcgtcc agtccggttt acaagtcaat gctttggaaa tgttggatac 2580
cgaaatgatg caattcgtca accaatctgg taatgtcacc aagaaatatg cagagtcgcc 2640
aactttaatg atgaaattgg gaggtaattc caagactgtg gttgatgcgc ttactaagga 2700
tgtgaagagt atctgtaccg agtgtaacat tggtcgcaag gatttcaaat ttgccgaaga 2760
tgaggaggaa aaggaggaat tatggactgc cagaaaagtt gcactttggt caactctaga 2820
tgcaggtaag gaaaaattgg gtcaagatgt tcaactgtgg acaaccgacg tggcagttcc 2880
aatttctaag ctggttgaca gcttggagaa cactagaagg ttactcaatg aagccggatt 2940
catgtcttcc atagtttcgc atgcaggtga tggtaattat cacgccttta ttgtgtacga 3000
caaaaaggat tacgtaaaag ctgcaaatgt cgtcaaagaa atggtccatc atgccctcga 3060
gttggatggg acagcaactg gtgaacacgg tattggaata ggtaagagag aattcttgag 3120
agaagaacta ggggatgccc caattgattt gatgaggaag atcaaatttg cccttgatcc 3180
attgctatta ttaaacccag acaaagtatt caaaattgac cctgaagaca caagattatc 3240
ttgattaatt aaacaggccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc 3300
ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct 3360
gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata 3420
tttcaaattt ttcttttttt tctgtacaaa cgcgtgtacg catgtaacgg gcagacggcc 3480
ggccataact tcgtataatg tatgctatac gaagttatgg caacggttca tcatctcatg 3540
gatctgcaca tgaacaaaca ccagagtcaa acgacgttga aattgaggct actgcgccaa 3600
ttgatgacaa tacagacgat gataacaaac cgaagttatc tgatgtagaa aaggattaga 3660
gatgctaaga gatagtgatg atatttcata aataatgtaa ttctatatat gttaattacc 3720
ttttttgcga ggcatattta tggtgaagga taagttttga ccatcaaaga aggttaatgt 3780
ggctgtggtt tcagggtcca taaagctttt caattcatct tttttttttt tgttcttttt 3840
tttgattccg gtttctttga aatttttttg attcggtaat ctccgagcag aaggaagaac 3900
gaaggaagga gcacagactt agattggtat atatacgcat atgtggtgtt gaagaaacat 3960
gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat 4020
aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg 4080
ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc 4140
gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa 4200
aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg 4260
cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta 4320
atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta 4380
cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcggaag 4440
aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc 4500
tagctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg 4560
ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga 4620
ttatgacac 4629
<210> 46
<211> 567
<212> PRT
<213> 东方伊萨酵母(Issatchenkia orientalis)
<400> 46
Met Ile Met Leu Pro Leu Ser Arg Ala Ala Val His Leu Ser Arg Thr
1 5 10 15
Arg His Ser Ser Leu Val Ser Leu Arg His Ala Leu Ala Ile Pro Gln
20 25 30
Arg Val Gln Tyr Ser His His Arg His Tyr Ser Ala Asn Thr Glu Lys
35 40 45
Thr Ala Lys Thr Gly Ser Glu Lys Asp Pro Lys Ser Gln Ala Arg Lys
50 55 60
Asp Leu Gly Lys Ser Phe Leu Ser Thr Thr Ala Ile Leu Phe Ile Gly
65 70 75 80
Ala Leu Val Gly Thr Ser Trp Ala Ser Phe Gln Leu Cys Lys Asn Pro
85 90 95
Pro Glu Phe Leu Phe Pro His Thr Ser Thr Phe Pro Leu Lys Asp Ala
100 105 110
Met Pro Pro Leu Tyr Gly Asp Ser Gln Lys Val Ile Asp Glu Leu Lys
115 120 125
Pro Leu Leu Arg Glu Asp Gln Ile Thr Ile Ser Lys Ser Glu Leu Asp
130 135 140
Thr His Ser Asp Ala Tyr Phe Ser Thr Asp His Pro Ser Asp Glu Glu
145 150 155 160
Arg Pro Asp Ala Ile Val Tyr Pro Glu Ser Thr Glu Gln Val Ser Gln
165 170 175
Ile Leu Lys Ile Ala His Asn His His Val Pro Ile Val Pro Tyr Thr
180 185 190
Gly Gly Thr Ser Leu Glu Gly His Tyr Ile Pro Thr Arg Lys Gly Ile
195 200 205
Cys Val Asp Leu Ser Arg Leu Asn Lys Val Leu Ala Leu His Lys Glu
210 215 220
Asp Leu Asp Ile Val Val Gln Pro Ala Val Gly Trp Glu Asp Leu Asp
225 230 235 240
Glu Phe Leu Arg Pro Gln Gly Leu Leu Phe Gly Pro Asp Pro Gly Pro
245 250 255
Gly Ala Cys Ile Gly Gly Met Cys Gly Thr Ser Cys Ser Gly Thr Asn
260 265 270
Ala Ala Arg Tyr Gly Thr Met Arg Glu Asn Val Val Ser Leu Thr Val
275 280 285
Val Leu Ala Asp Gly Thr Leu Gly Ile Val Thr Glu Ala Thr Leu Lys
290 295 300
Leu His Val Ala Pro Lys Phe Glu Asn Val Ala Val Val Asn Phe Pro
305 310 315 320
Thr Leu Arg Asp Ala Ala Asn Ala Val Ala Lys Ile Val Gln Ser Gly
325 330 335
Leu Gln Val Asn Ala Leu Glu Met Leu Asp Thr Glu Met Met Gln Phe
340 345 350
Val Asn Gln Ser Gly Asn Val Thr Lys Lys Tyr Ala Glu Ser Pro Thr
355 360 365
Leu Met Met Lys Leu Gly Gly Asn Ser Lys Thr Val Val Asp Ala Leu
370 375 380
Thr Lys Asp Val Lys Ser Ile Cys Thr Glu Cys Asn Ile Gly Arg Lys
385 390 395 400
Asp Phe Lys Phe Ala Glu Asp Glu Glu Glu Lys Glu Glu Leu Trp Thr
405 410 415
Ala Arg Lys Val Ala Leu Trp Ser Thr Leu Asp Ala Gly Lys Glu Lys
420 425 430
Leu Gly Gln Asp Val Gln Leu Trp Thr Thr Asp Val Ala Val Pro Ile
435 440 445
Ser Lys Leu Val Asp Ser Leu Glu Asn Thr Arg Arg Leu Leu Asn Glu
450 455 460
Ala Gly Phe Met Ser Ser Ile Val Ser His Ala Gly Asp Gly Asn Tyr
465 470 475 480
His Ala Phe Ile Val Tyr Asp Lys Lys Asp Tyr Val Lys Ala Ala Asn
485 490 495
Val Val Lys Glu Met Val His His Ala Leu Glu Leu Asp Gly Thr Ala
500 505 510
Thr Gly Glu His Gly Ile Gly Ile Gly Lys Arg Glu Phe Leu Arg Glu
515 520 525
Glu Leu Gly Asp Ala Pro Ile Asp Leu Met Arg Lys Ile Lys Phe Ala
530 535 540
Leu Asp Pro Leu Leu Leu Leu Asn Pro Asp Lys Val Phe Lys Ile Asp
545 550 555 560
Pro Glu Asp Thr Arg Leu Ser
565
<210> 47
<211> 4199
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和植物乳杆菌。
<400> 47
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atggtcgcca ttgatttgcc 1560
atacgacaag agaactatta ctgctcaaat tgacgatgag aactacgcgg gcaagttggt 1620
gagccaggct gccacctacc acaacaaact gtcagaacag gaaacagttg agaaaagttt 1680
ggataaccca ataggatctg ataagctaga ggaacttgca cgtggtaaac ataacattgt 1740
gattatctca tctgatcata ccagacctgt tccatcacac atcatcacac ctattttgct 1800
tagaagatta agatctgtcg ctcctgacgc ccgtattaga atcttagttg caactggatt 1860
tcatagacca tctacccatg aggaactcgt taacaagtac ggtgaagata tcgtgaacaa 1920
tgaggaaatt gtcatgcatg tatctacaga tgactcatct atggtaaaga ttggtcaatt 1980
gccatccggc ggtgactgta tcatcaataa ggttgcagca gaagctgact tactaatatc 2040
cgaagggttc attgaatcac acttctttgc tgggttttct ggaggtagaa agtctgttct 2100
gccaggcatc gcaagttata agacaataat ggctaaccat agtggggagt tcattaacag 2160
ccctaaagcc agaactggca acttgatgca caattcaatt cataaagata tggtatacgc 2220
tgctagaaca gcaaaacttg cgttcattat caacgttgta ttagatgagg acaaaaagat 2280
aataggttca ttcgcgggtg atatggaagc agctcacaag gttgggtgcg atttcgtaaa 2340
ggaactgtcc tccgttccag ccatcgattg tgatatagca atttcaacaa acggaggtta 2400
ccctttggat caaaacattt accaggcggt gaagggtatg acagccgcag aagcaactaa 2460
taaggaaggt ggcactatca tcatggtcgc cggtgctaga gatggtcatg gaggcgaagg 2520
tttctatcat aatctagcag atgttgacga cccaaaggaa ttcttagatc aagcaatcaa 2580
tacacctaga ctaaagacaa ttccagatca atggacagct caaatctttg ccagaatact 2640
tgttcaccat catgtgatct ttgtttctga tctcgtcgat ccagatttga tcactaatat 2700
gcacatggaa ttggctaaaa ccttagatga ggcaatggag aaggcttacg ctagagaagg 2760
tcaagcagcc aaagtcacag tcattccaga cggattaggt gttattgtta agtagttaat 2820
taaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca 2880
cgccctcctc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 2940
gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 3000
tttctttttt ttctgtacaa acgcgtgtac gcatgtaacg ggcagacggc cggccataac 3060
ttcgtataat gtatgctata cgaagttatg gcaacggttc atcatctcat ggatctgcac 3120
atgaacaaac accagagtca aacgacgttg aaattgaggc tactgcgcca attgatgaca 3180
atacagacga tgataacaaa ccgaagttat ctgatgtaga aaaggattag agatgctaag 3240
agatagtgat gatatttcat aaataatgta attctatata tgttaattac cttttttgcg 3300
aggcatattt atggtgaagg ataagttttg accatcaaag aaggttaatg tggctgtggt 3360
ttcagggtcc ataaagcttt tcaattcatc tttttttttt ttgttctttt ttttgattcc 3420
ggtttctttg aaattttttt gattcggtaa tctccgagca gaaggaagaa cgaaggaagg 3480
agcacagact tagattggta tatatacgca tatgtggtgt tgaagaaaca tgaaattgcc 3540
cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 3600
cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 3660
ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 3720
aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 3780
tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 3840
ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 3900
aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 3960
acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcggaa gaagtaacaa 4020
aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctagctactg 4080
gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 4140
ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgaca 4199
<210> 48
<211> 424
<212> PRT
<213> 植物乳杆菌(Lactobacillus plantarum)
<400> 48
Met Val Ala Ile Asp Leu Pro Tyr Asp Lys Arg Thr Ile Thr Ala Gln
1 5 10 15
Ile Asp Asp Glu Asn Tyr Ala Gly Lys Leu Val Ser Gln Ala Ala Thr
20 25 30
Tyr His Asn Lys Leu Ser Glu Gln Glu Thr Val Glu Lys Ser Leu Asp
35 40 45
Asn Pro Ile Gly Ser Asp Lys Leu Glu Glu Leu Ala Arg Gly Lys His
50 55 60
Asn Ile Val Ile Ile Ser Ser Asp His Thr Arg Pro Val Pro Ser His
65 70 75 80
Ile Ile Thr Pro Ile Leu Leu Arg Arg Leu Arg Ser Val Ala Pro Asp
85 90 95
Ala Arg Ile Arg Ile Leu Val Ala Thr Gly Phe His Arg Pro Ser Thr
100 105 110
His Glu Glu Leu Val Asn Lys Tyr Gly Glu Asp Ile Val Asn Asn Glu
115 120 125
Glu Ile Val Met His Val Ser Thr Asp Asp Ser Ser Met Val Lys Ile
130 135 140
Gly Gln Leu Pro Ser Gly Gly Asp Cys Ile Ile Asn Lys Val Ala Ala
145 150 155 160
Glu Ala Asp Leu Leu Ile Ser Glu Gly Phe Ile Glu Ser His Phe Phe
165 170 175
Ala Gly Phe Ser Gly Gly Arg Lys Ser Val Leu Pro Gly Ile Ala Ser
180 185 190
Tyr Lys Thr Ile Met Ala Asn His Ser Gly Glu Phe Ile Asn Ser Pro
195 200 205
Lys Ala Arg Thr Gly Asn Leu Met His Asn Ser Ile His Lys Asp Met
210 215 220
Val Tyr Ala Ala Arg Thr Ala Lys Leu Ala Phe Ile Ile Asn Val Val
225 230 235 240
Leu Asp Glu Asp Lys Lys Ile Ile Gly Ser Phe Ala Gly Asp Met Glu
245 250 255
Ala Ala His Lys Val Gly Cys Asp Phe Val Lys Glu Leu Ser Ser Val
260 265 270
Pro Ala Ile Asp Cys Asp Ile Ala Ile Ser Thr Asn Gly Gly Tyr Pro
275 280 285
Leu Asp Gln Asn Ile Tyr Gln Ala Val Lys Gly Met Thr Ala Ala Glu
290 295 300
Ala Thr Asn Lys Glu Gly Gly Thr Ile Ile Met Val Ala Gly Ala Arg
305 310 315 320
Asp Gly His Gly Gly Glu Gly Phe Tyr His Asn Leu Ala Asp Val Asp
325 330 335
Asp Pro Lys Glu Phe Leu Asp Gln Ala Ile Asn Thr Pro Arg Leu Lys
340 345 350
Thr Ile Pro Asp Gln Trp Thr Ala Gln Ile Phe Ala Arg Ile Leu Val
355 360 365
His His His Val Ile Phe Val Ser Asp Leu Val Asp Pro Asp Leu Ile
370 375 380
Thr Asn Met His Met Glu Leu Ala Lys Thr Leu Asp Glu Ala Met Glu
385 390 395 400
Lys Ala Tyr Ala Arg Glu Gly Gln Ala Ala Lys Val Thr Val Ile Pro
405 410 415
Asp Gly Leu Gly Val Ile Val Lys
420
<210> 49
<211> 5084
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和构巢曲霉。
<400> 49
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgctaaaat acaaaccttt 1560
actaaaaatc tcgaagaact gtgaggctgc tatcctcaga gcgtctaaga ctagattgaa 1620
cacaatccgc gcgtacggtt ctaccgttcc aaaatccaag tcgttcgaac aagactcaag 1680
aaaacgcaca cagtcatgga ctgccttgag agtcggtgca attctagccg ctactagttc 1740
cgtggcgtat ctaaactggc ataatggcca aatagacaac gagccgaaac tggatatgaa 1800
taaacaaaag atttcgcccg ctgaagttgc caagcataac aagcccgatg attgttgggt 1860
tgtgatcaat ggttacgtat acgacttaac gcgattccta ccaaatcatc caggtgggca 1920
ggatgttatc aagtttaacg ccgggaaaga tgtcactgct atttttgaac cactacatgc 1980
tcctaatgtc atcgataagt atatagctcc cgagaaaaaa ttgggtcccc ttcaaggatc 2040
catgcctcct gaacttgtct gtcctcctta tgctcctggt gaaactaagg aagatatcgc 2100
tagaaaagaa caactaaaat cgctgctacc tcctctagat aatattatta acctttacga 2160
ctttgaatac ttggcctctc aaactttgac taaacaagcg tgggcctact attcctccgg 2220
tgctaacgac gaagttactc acagagaaaa ccataatgct tatcatagga tttttttcaa 2280
accaaagatc cttgtagatg tacgcaaagt agacatttca actgacatgt tgggttctca 2340
tgtggatgtt cccttctacg tgtctgctac agctttgtgt aaactgggaa accccttaga 2400
aggtgaaaaa gatgtcgcca gaggttgtgg ccaaggtgtg acaaaagtcc cacaaatgat 2460
atctactttg gcttcatgtt cccctgagga aattattgaa gcagcaccct ctgataaaca 2520
aattcaatgg taccaactat atgttaactc tgatagaaag atcactgatg atttggttaa 2580
aaatgtagaa aagctgggtg taaaggcatt atttgtcact gtggatgctc caagtttagg 2640
tcaaagagaa aaagatatga agctgaaatt ttccaataca aaggctggtc caaaagcgat 2700
gaagaaaact aatgtagaag aatctcaagg tgcttcgaga gcgttatcaa agtttattga 2760
cccctctttg acttggaaag atatagaaga gttgaagaaa aagacaaaac tacctattgt 2820
tatcaaaggt gttcaacgta ccgaagatgt tatcaaagca gcagaaatcg gtgtaagtgg 2880
ggtggttcta tccaatcatg gtggtagaca attagatttt tcaagggctc ccattgaagt 2940
cctggctgaa accatgccaa tcctggaaca acgtaacttg aaggataagt tggaagtttt 3000
cgtggacggt ggtgttcgtc gtggtacaga tgtcttgaaa gcgttatgtc taggtgctaa 3060
aggtgttggt ttgggtagac cattcttgta tgcgaactca tgctatggtc gtaatggtgt 3120
tgaaaaagcc attgaaattt taagagatga aattgaaatg tctatgagac tattaggtgt 3180
tactagcatt gcggaattga agcctgatct tttagatcta tcaacactaa aggcaagaac 3240
agttggagta ccaaacgacg tgctgtataa tgaagtttat gagggaccta ctttaacaga 3300
atttgaggat gcatgattaa ttaaacaggc cccttttcct ttgtcgatat catgtaatta 3360
gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga 3420
gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 3480
acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac 3540
gggcagacgg ccggccataa cttcgtataa tgtatgctat acgaagttat ccttacatca 3600
cacccaatcc cccacaagtg atcccccaca caccatagct tcaaaatgtt tctactcctt 3660
ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa 3720
gcacagcata ctaaatttcc cctctttctt cctctagggt ggcgttaatt acccgtacta 3780
aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat 3840
aaaaattttt atcacgtttc tttttcttga aaaatttttt ttttgatttt tttctctttc 3900
gatgacctcc cattgatatt taagttaata aatggtcttc aatttctcaa gtttcagttt 3960
cgtttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc 4020
aatctaatct aagttttaat tacaaaatgc cacaatcctg ggaagaattg gccgccgaca 4080
aacgtgcccg tttggctaaa accattcctg acgaatggaa ggttcaaact ttgcctgccg 4140
aagattccgt tattgatttc ccaaagaagt ccggtatttt gtctgaggct gaattgaaga 4200
ttaccgaagc ctctgctgct gatttggtct ccaagttggc cgctggtgag ttgacttctg 4260
ttgaagtcac tttggctttt tgtaagagag ctgctattgc tcaacaatta accaactgtg 4320
ctcacgaatt cttcccagat gctgctttag ctcaagctag agaattagat gaatactacg 4380
ctaagcataa gagaccagtt ggtccattac acggtttacc aatctcttta aaggaccaat 4440
tgcgtgttaa gggttacgaa acctccatgg gttacatttc ctggttaaac aaatacgatg 4500
aaggtgattc cgtcttaacc accatgttga gaaaagctgg tgctgttttc tacgttaaga 4560
cctctgtccc acaaaccttg atggtctgtg aaaccgtcaa caacatcatt ggtagaactg 4620
tcaatccaag aaacaaaaat tggtcctgtg gtggttcttc tggtggtgaa ggtgctattg 4680
ttggtattag aggtggtgtt attggtgtcg gtactgacat tggtggttcc attagagtcc 4740
cagctgcttt caacttttta tacggtttga gaccatctca cggtagattg ccatatgcta 4800
aaatggctaa ctctatggaa ggtcaagaaa ccgttcactc cgtcgttggt cctatcactc 4860
actccgtcga agacttgaga ttgttcacca aatctgtctt gggtcaagaa ccttggaagt 4920
acgactctaa ggtcatcccc atgccatgga gacaatctga atctgacatc attgcctcta 4980
agattaagaa tggtggtttg aacattggtt attacaattt cgacggtaac gtcttgccac 5040
acccaccaat tttacgtggt gtcgaaacta ccgttgccgc tttg 5084
<210> 50
<211> 5343
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、乳酸克鲁维酵母和构巢曲霉。
<400> 50
gaaggtgcta ttgttggtat tagaggtggt gttattggtg tcggtactga cattggtggt 60
tccattagag tcccagctgc tttcaacttt ttatacggtt tgagaccatc tcacggtaga 120
ttgccatatg ctaaaatggc taactctatg gaaggtcaag aaaccgttca ctccgtcgtt 180
ggtcctatca ctcactccgt cgaagacttg agattgttca ccaaatctgt cttgggtcaa 240
gaaccttgga agtacgactc taaggtcatc ccaatgccat ggagacaatc tgaatctgac 300
atcattgcct ctaagattaa gaatggtggt ttgaacattg gttattacaa tttcgacggt 360
aacgtcttgc cacacccacc aattttacgt ggtgtcgaaa ctaccgttgc cgctttggcc 420
aaggctggtc acaccgttac tccatggact ccatacaagc atgatttcgg tcatgacttg 480
atttcccaca tctatgctgc tgatggttct gccgacgtca tgagagacat ttctgcctct 540
ggtgagccag ccatccctaa cattaaggac ttgttgaacc caaatattaa ggctgttaac 600
atgaacgaat tgtgggacac tcatttacaa aagtggaact atcaaatgga atacttggaa 660
aagtggcgtg aagctgaaga aaaagctggt aaggaattgg acgctattat cgctccaatt 720
actcctaccg ccgctgtcag acacgatcaa ttcagatact acggttacgc ctccgttatt 780
aacttattgg atttcacctc tgttgtcgtc ccagtcactt tcgctgataa gaatattgat 840
aagaagaacg aatcttttaa agctgtttcc gaattggatg ctttggttca agaagaatac 900
gacccagagg cttatcacgg tgctcctgtt gctgttcaag ttattggtag aagattgtcc 960
gaagagagaa ctttggctat cgccgaagaa gtcggtaaat tgttgggtaa cgtcgtcact 1020
ccataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata 1080
agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt 1140
aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac 1200
cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg 1260
tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga 1320
ggacaacaca taacttcgta taatgtatgc tatacgaagt tatctcgaga tctcccctaa 1380
accgtggaat atttcggata tccttttgtt gtttccgggt gtacaatatg gacttcctct 1440
tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac 1500
atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac ataatgggct 1560
aaacaagact acaccattta cactgcctca ttgatggtgg tacataacga actaatactg 1620
tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc 1680
atctattgaa gtaataatag gcgcatgcaa cttctttttt tttttttttt ttctctctcc 1740
cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa 1800
aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc 1860
tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac 1920
ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc 1980
tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt 2040
ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca 2100
actatctcat atacatctag aatgaataac aacaacatta caccagagtc agactcaatg 2160
aagagtaacg ataacgatca aaccaatgat tacatgccag atgtggctga tttcgatcat 2220
acacaaacaa acacaaacga aattgctaga gcaatttctc acccaggttc tgttctgtca 2280
agagttgcaa gctacgtcag cagaaaggac agatacgtag acgagaatgg caatgaagtt 2340
tggcaagacg atgaagtgag tatcctaatg gaggaagatg agactcctga tttcacatgg 2400
aaaaacatta gacattacgc tatcactaga ttcactacat tgacagaact tcacagagtg 2460
tctatggaaa acatcaaccc aattccagaa ttgagaaaaa tgacattaca taattggaac 2520
tacttcttta tggggtatgc cgcgtggttg tgtgctgctt gggcattttt cgccgtttcc 2580
gttagtacag cccctcttgc aaccctttat ggcaaggaaa caaaagacat ttcatggggt 2640
ctatccttag tcttattcgt cagatctgct ggagcgatta tctttggcat ctggaccgac 2700
aattactcca gaaagtggcc atacattaca tgcttaggtc ttttcttgat ctgtcagctt 2760
tgcacacctt gggcaaagac ctacactcaa ttcttgggag ttcgttggat ttccggtatc 2820
gcaatgggtg gaatttacgc ctgtgcttct gcaacagcga ttgaggatgc accagtaaag 2880
gctcgttctt tcttatccgg cttattcttc actgcctacg ccatgggttt tatcttcgca 2940
ataatctttt acagagcatt cttaaacgtc aacggtgaaa actactggaa ggttcaattc 3000
tggttttcaa tatggttacc agctgtactg atattgtgga gactcgtatg gccagaaact 3060
aagtacttca ccaaagtact aaaagctaga caactaatgc gtgatgatgc aatagccaaa 3120
aacggtggtc aaccactccc aaagttgtct ttcaagcaaa agttcgccaa tgtgaaaaag 3180
accgttagta aatactggct tttgtttggt tacttaattc tcttgttggt tggtcctaac 3240
tacctaacac acgcctctca agacttgttt cctacaatgt taagagcaca attgagattt 3300
tctgaagatg ctgtcaccgt ggccattgta gttgtttgtc tgggtagcat tgctgggggg 3360
atgtttttcg gacagttgat ggagatcact ggtagaagag ttggcctact attggcccta 3420
atcatggctg gctgctttac ttaccctgcc ttcatgctta aaacttcatc tgcagtgctg 3480
ggggcaggct ttatgctctg gttctcaatc ttaggtgtgt ggggtgtttt accaatccat 3540
ttatcagaat tgtcccctcc agaagctaga gcattagtct ctggattagc atatcagctg 3600
ggtaatctag cttcagcagc ttctgttgtt atagagaatg atttggcgga tttgtatcca 3660
atagaatgga attctgctgg agaagttaca aacaaggact actctaaggt aatggctatt 3720
cttacaggtt cttcagtcat tttcactttc gtgttggttt tcgttggtca cgaaaagttt 3780
catagagatt tgtcatcccc acatcttaag tcatacatcg agagagtcga tcaaacagaa 3840
gaggtcgcag ctatgactgg atctactgcg aactctatct ctagtaagcc ttcagatgat 3900
cagctcgaaa aagtttcagt ctagttaatt aatttaccag cttactatcc ttcttgaaaa 3960
tatgcactct atatctttta gttcttaatt gcaacacata gatttgctgt ataacgaatt 4020
ttatgctatt tttttaattt ggagttcggt gatgaaagtg tcacagcgaa tttcctcaca 4080
tgtagggacc gaattgttta caagttctct gtaccaccat ggagacatca aagattgaaa 4140
atctatggaa agatatggac ggtagcaaca agaatatagc acgagccgcg gagttcattt 4200
cgttactttt gatatcgctc acaactattg cgaagcgctt cagtgaaaaa atcataagga 4260
aaagttgtaa atattattgg tagtattcgt ttggtaaagt agagggggta atttttcccc 4320
tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag ctatacttcg 4380
gagcactgtt gagcgaaggc tcaggccggc atatgacgtt ttattacctt tgatcacatt 4440
tccacgccat ttcgcattct caccctcata agtcatacac cgaaaagaaa gtttaaggga 4500
tcaatgagct tactataatc tcagtatatt tatttttatc gatgattcac cacaacaatc 4560
ttgctcccga aaagaaagca gacggagtag aagcatttga aactccttca gaccttcaag 4620
tatatatata tatatatata tatgtatatg tgtacatttt cacgctaata ctaatgtata 4680
attagaagat aatttttact catttttcgt tatcttcacg tcacccgaac ctagaaccaa 4740
atgtcatttt cacgatatgt aaatagtgaa ataggcaaaa acgccaaaaa gtagtaagcg 4800
caacatacac taaaccatta aagaatatct cgaccagaat ctaacagata tacatgttcc 4860
gataatgtct gagttaggtg agtattctaa attagaaaac aaagagctta gaacggagtt 4920
tgaattgaca aattttcctt ttccaggcac aactgataac gactccgatg acggaagcca 4980
agggcagaac tctttgaata tcattactcc tgacatggat gatactctgg ttaatgatgt 5040
acttcgagaa aacgataaaa agtctagtat gagaatggct tttatgaatc tagcaaactc 5100
tattcttggt gccggaataa ttactcagcc gttcgcgatc aaaaatgctg gtatattagg 5160
cgggctatta tcatacgtag ccctcggatt tatagttgat tggacgttaa gacttattgt 5220
cattaacttg actcttgctg gcaagagaac ataccagggt acggtcgaac atgtaatggg 5280
taaaaaaggg aaattgctga ttctatttac aaacgggtta tttgcatttg gtggatgtat 5340
tgg 5343
<210> 51
<211> 562
<212> PRT
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 51
Met Asn Val Phe Gly Lys Lys Glu Glu Lys Gln Glu Lys Val Tyr Ser
1 5 10 15
Leu Gln Asn Gly Phe Pro Tyr Ser His His Pro Tyr Ala Ser Gln Tyr
20 25 30
Ser Arg Pro Asp Gly Pro Ile Leu Leu Gln Asp Phe His Leu Leu Glu
35 40 45
Asn Ile Ala Ser Phe Asp Arg Glu Arg Val Pro Glu Arg Val Val His
50 55 60
Ala Lys Gly Gly Gly Cys Arg Leu Glu Phe Glu Leu Thr Asp Ser Leu
65 70 75 80
Ser Asp Ile Thr Tyr Ala Ala Pro Tyr Gln Asn Val Gly Tyr Lys Cys
85 90 95
Pro Gly Leu Val Arg Phe Ser Thr Val Gly Gly Glu Ser Gly Thr Pro
100 105 110
Asp Thr Ala Arg Asp Pro Arg Gly Val Ser Phe Lys Phe Tyr Thr Glu
115 120 125
Trp Gly Asn His Asp Trp Val Phe Asn Asn Thr Pro Val Phe Phe Leu
130 135 140
Arg Asp Ala Ile Lys Phe Pro Val Phe Ile His Ser Gln Lys Arg Asp
145 150 155 160
Pro Gln Ser His Leu Asn Gln Phe Gln Asp Thr Thr Ile Tyr Trp Asp
165 170 175
Tyr Leu Thr Leu Asn Pro Glu Ser Ile His Gln Ile Thr Tyr Met Phe
180 185 190
Gly Asp Arg Gly Thr Pro Ala Ser Trp Ala Ser Met Asn Ala Tyr Ser
195 200 205
Gly His Ser Phe Ile Met Val Asn Lys Glu Gly Lys Asp Thr Tyr Val
210 215 220
Gln Phe His Val Leu Ser Asp Thr Gly Phe Glu Thr Leu Thr Gly Asp
225 230 235 240
Lys Ala Ala Glu Leu Ser Gly Ser His Pro Asp Tyr Asn Gln Ala Lys
245 250 255
Leu Phe Thr Gln Leu Gln Asn Gly Glu Lys Pro Lys Phe Asn Cys Tyr
260 265 270
Val Gln Thr Met Thr Pro Glu Gln Ala Thr Lys Phe Arg Tyr Ser Val
275 280 285
Asn Asp Leu Thr Lys Ile Trp Pro His Lys Glu Phe Pro Leu Arg Lys
290 295 300
Phe Gly Thr Ile Thr Leu Thr Glu Asn Val Asp Asn Tyr Phe Gln Glu
305 310 315 320
Ile Glu Gln Val Ala Phe Ser Pro Thr Asn Thr Cys Ile Pro Gly Ile
325 330 335
Lys Pro Ser Asn Asp Ser Val Leu Gln Ala Arg Leu Phe Ser Tyr Pro
340 345 350
Asp Thr Gln Arg His Arg Leu Gly Ala Asn Tyr Gln Gln Leu Pro Val
355 360 365
Asn Arg Pro Arg Asn Leu Gly Cys Pro Tyr Ser Lys Gly Asp Ser Gln
370 375 380
Tyr Thr Ala Glu Gln Cys Pro Phe Lys Ala Val Asn Phe Gln Arg Asp
385 390 395 400
Gly Pro Met Ser Tyr Tyr Asn Phe Gly Pro Glu Pro Asn Tyr Ile Ser
405 410 415
Ser Leu Pro Asn Gln Thr Leu Lys Phe Lys Asn Glu Asp Asn Asp Glu
420 425 430
Val Ser Asp Lys Phe Lys Gly Ile Val Leu Asp Glu Val Thr Glu Val
435 440 445
Ser Val Arg Lys Gln Glu Gln Asp Gln Ile Arg Asn Glu His Ile Val
450 455 460
Asp Ala Lys Ile Asn Gln Tyr Tyr Tyr Val Tyr Gly Ile Ser Pro Leu
465 470 475 480
Asp Phe Glu Gln Pro Arg Ala Leu Tyr Glu Lys Val Tyr Asn Asp Glu
485 490 495
Gln Lys Lys Leu Phe Val His Asn Val Val Cys His Ala Cys Lys Ile
500 505 510
Lys Asp Pro Lys Val Lys Lys Arg Val Thr Gln Tyr Phe Gly Leu Leu
515 520 525
Asn Glu Asp Leu Gly Lys Val Ile Ala Glu Cys Leu Gly Val Pro Trp
530 535 540
Glu Pro Val Asp Leu Glu Gly Tyr Ala Lys Thr Trp Ser Ile Ala Ser
545 550 555 560
Ala Asn
<210> 52
<211> 524
<212> PRT
<213> 解脂耶氏酵母(Yarrowia lipolytica)
<400> 52
Met Glu Ala Pro Asn Leu Ser Pro Ala Ser Ile Arg Arg Tyr Phe Ala
1 5 10 15
Thr Arg Leu Pro Thr Leu Ile Pro Gln Lys Leu Thr Pro Glu Glu Lys
20 25 30
Lys Leu Leu Asn Pro Phe Pro Ala Leu Ala Leu Ile Asn Lys Lys Thr
35 40 45
Trp Leu Phe Ile Ala Cys Ala Phe Cys Gly Trp Thr Trp Asp Ala Phe
50 55 60
Asp Phe Phe Ser Val Gly Leu Val Ala Pro Glu Ile Ala Lys Ser Leu
65 70 75 80
Asn Arg Ser Val Thr Asp Ile Thr Trp Gly Ile Thr Leu Val Leu Met
85 90 95
Leu Arg Ser Ile Gly Ala Val Val Phe Gly Ile Ala Ser Asp Arg Tyr
100 105 110
Gly Arg Lys Trp Pro Phe Ile Val Asn Leu Leu Cys Phe Ile Ala Leu
115 120 125
Glu Leu Gly Ser Gly Phe Val Gln Thr Tyr Lys Gln Phe Leu Gly Val
130 135 140
Arg Ala Ile Tyr Gly Ile Ala Met Gly Gly Leu Tyr Gly Asn Ala Ala
145 150 155 160
Ala Thr Ala Leu Glu Asp Cys Pro Pro Gln Ala Arg Gly Ile Ile Ser
165 170 175
Gly Phe Leu Gln Ser Gly Tyr Ala Leu Gly Tyr Leu Leu Cys Val Val
180 185 190
Phe Thr Arg Ala Ile Ala Asp Thr Ser Pro Tyr Gly Trp Arg Ala Leu
195 200 205
Phe Trp Phe Gly Ser Gly Pro Pro Val Leu Phe Ile Ile Phe Arg Tyr
210 215 220
Phe Leu Pro Glu Thr Glu Thr Tyr Leu Ala Ser Lys Ala Ser Gln Glu
225 230 235 240
Glu Ala Gly Ile Glu Lys Lys Phe Trp Asn Gly Ile Lys Val Thr Phe
245 250 255
Lys Asn Tyr Trp Leu Met Phe Ile Tyr Leu Val Ile Leu Met Ala Gly
260 265 270
Phe Asn Phe Met Ser His Gly Ser Gln Asp Leu Tyr Pro Thr Met Leu
275 280 285
Lys Asn Gln Arg His Phe Ser Ala Asp Arg Ser Thr Val Thr Asn Cys
290 295 300
Val Ala Asn Phe Gly Ala Ile Ala Gly Gly Met Leu Ile Gly His Phe
305 310 315 320
Ser Thr Ala Leu Gly Arg Arg Leu Ser Ile Met Ile Ser Cys Val Ile
325 330 335
Gly Gly Ala Leu Ile Tyr Pro Trp Ala Phe Val Gly Asn Ser Ala Gly
340 345 350
Thr Asn Ala Gly Val Phe Phe Leu Gln Phe Phe Val Gln Gly Ala Trp
355 360 365
Gly Val Val Pro Ile His Leu Ser Glu Leu Ser Pro Pro Glu Leu Arg
370 375 380
Ser Ser Met Val Gly Ile Ala Tyr Gln Met Gly Asn Leu Ala Ser Ser
385 390 395 400
Ala Ser Ser Thr Ile Glu Ser Lys Ile Gly Glu Arg Phe Pro Leu Lys
405 410 415
Asn Ala Lys Gly Glu Phe Glu Lys Gly Phe Tyr Asp Tyr Gly Lys Val
420 425 430
Met Ala Ile Phe Met Gly Cys Val Phe Gly Phe Val Leu Ile Val Thr
435 440 445
Phe Val Gly Pro Glu Asn Arg Gly Ala Thr Met Leu Thr Glu Asp Ala
450 455 460
Gln Met Met Val Asp Ala Glu His Arg Leu Asp Ala Glu Glu Lys Gly
465 470 475 480
Asp Phe Glu Asn Leu Glu Arg Val Asp Ser Glu Gly Lys Gln Met Asp
485 490 495
Asn Phe Val Glu Glu Val Ala Glu Pro Glu Gly Val Tyr Thr Gly Ser
500 505 510
His Pro Pro Gln Tyr Asp Ser Pro Tyr Glu Ser Lys
515 520
<210> 53
<211> 4656
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和克鲁弗酵母。
<400> 53
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga ttgcatcctc gcttacttcg 1560
tggattgacc tcgaagaaat ctgtgatcaa cggatctaaa caactccgtc tcgcgtcaac 1620
tttgcgcaaa acttcccatg gttcctattc caaaaacagg ttttctaatg gtttgactgc 1680
tggtgctatt gctactgctg ctattggtgt atctctgtat tcttactcca ttgaaaacgg 1740
tactgttacg gactcttcca aaaaaccaat cgaccctcaa gatgtcgcta aacacaatga 1800
tccaaaggac tgctgggtag tcatcgacgg atacgtttat gatttgaccg agtttattca 1860
ttctcatcca ggtggtccaa ccattatcga aaataacgcc ggtaaagacg ttaccgcaat 1920
ttttggtcca attcatgctc cagacgtgat tgaaaaatat attgctcccg agaagagaat 1980
tggcccgttg gatggcgtca tgccacagga tttggtctgt tctcctctga ctcctggtga 2040
aacaccggac gacattgtcc gcaaagaaga actgcgtagc cgtctcccca gtttggacgc 2100
attggtcaac ctgtatgact ttgaatactt ggcgtcgcaa atattgacca agcaggcatg 2160
gtcttactac tcgtctgctg cagacgatga agtcacccat agggaaaacc atgcagctta 2220
ccaccgtata tttttcaaac caaaggtctt ggtcgatgtc agagaggttg atacttcaac 2280
gaaaatttta ggccaagatg ttcctgtgcc attttacgtt tctgcaaccg cgctatgtaa 2340
acttgggaat ccaaaggaag gtgaaaagga tgtcgcgcgc ggttgtggcc aagggccgat 2400
caagtgtcct caaatgattt ccacgttggc gtcatgttcc ttacaggaaa tcgtcgaagc 2460
agccccatcg aaagaacaaa tccagtggtt ccaactgtac gtcaatacag acagaaagat 2520
taccgatgac ttgatcaagc aggttgaaaa attggggttg aaggccatct ttgtgactgt 2580
ggatgctcct tctctaggta gacgggaaaa ggacatgaag gttaagttta caaactctaa 2640
cggtgctaag gcaatggaaa agactaaagt caaggaatct aagggtgctt ccgcagcatt 2700
gtctaccttc atcgaccctg ctttgagttg ggacgatatc gttgaaatca agaaaaagac 2760
aaatttgcca attgtcatta agggtgttca acgtgtcgaa gatgtgttga aggctgcaga 2820
agtaggtgct gctggtgtcg ttctttccaa ccacggtggt aggcagctgg acttttcgcg 2880
tgcgccaatt gaagtgttgg ccgaatctgt gccagttttg aaagagcgca atctaagcga 2940
caagctggag atctttatcg acggtggtgt gcgtcgtggt aacgatgtgc tgaaggccct 3000
atgtttgggt gccaagggtg ttggtctcgg tagaccattt ttgtatgcca acagttgtta 3060
cggtaaagac ggtgttcaaa aggctataga aatgttgact gaagagattc aaatgtccat 3120
gagattgttg ggtgtcacaa agattgaaga tttgaagccc gaactactgg atatgagcag 3180
ctttagcggc aggactgtca acgttccaca agacagccta tacaggtctg tctatgaaaa 3240
gagtgaaacc gtacaatttt tggatgaatg attaattaaa caggcccctt ttcctttgtc 3300
gatatcatgt aattagttat gtcacgctta cattcacgcc ctcctcccac atccgctcta 3360
accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt 3420
atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacaaacgc 3480
gtgtacgcat gtaacgggca gacggccggc cataacttcg tataatgtat gctatacgaa 3540
gttatggcaa cggttcatca tctcatggat ctgcacatga acaaacacca gagtcaaacg 3600
acgttgaaat tgaggctact gcgccaattg atgacaatac agacgatgat aacaaaccga 3660
agttatctga tgtagaaaag gattagagat gctaagagat agtgatgata tttcataaat 3720
aatgtaattc tatatatgtt aattaccttt tttgcgaggc atatttatgg tgaaggataa 3780
gttttgacca tcaaagaagg ttaatgtggc tgtggtttca gggtccataa agcttttcaa 3840
ttcatctttt ttttttttgt tctttttttt gattccggtt tctttgaaat ttttttgatt 3900
cggtaatctc cgagcagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata 3960
tacgcatatg tggtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca 4020
gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg 4080
ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa 4140
caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag 4200
cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca 4260
tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg 4320
aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat 4380
acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg 4440
ttagcggttt gaagcaggcg gcggaagaag taacaaagga acctagaggc cttttgatgt 4500
tagcagaatt gtcatgcaag ggctccctag ctactggaga atatactaag ggtactgttg 4560
acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg 4620
gaagagatga aggttacgat tggttgatta tgacac 4656
<210> 54
<211> 423
<212> PRT
<213> 克鲁弗酵母(Saccharomyces kluyveri)
<400> 54
Met Pro Gln Asp Leu Val Cys Ser Pro Leu Thr Pro Gly Glu Thr Pro
1 5 10 15
Asp Asp Ile Val Arg Lys Glu Glu Leu Arg Ser Arg Leu Pro Ser Leu
20 25 30
Asp Ala Leu Val Asn Leu Tyr Asp Phe Glu Tyr Leu Ala Ser Gln Ile
35 40 45
Leu Thr Lys Gln Ala Trp Ser Tyr Tyr Ser Ser Ala Ala Asp Asp Glu
50 55 60
Val Thr His Arg Glu Asn His Ala Ala Tyr His Arg Ile Phe Phe Lys
65 70 75 80
Pro Lys Val Leu Val Asp Val Arg Glu Val Asp Thr Ser Thr Lys Ile
85 90 95
Leu Gly Gln Asp Val Pro Val Pro Phe Tyr Val Ser Ala Thr Ala Leu
100 105 110
Cys Lys Leu Gly Asn Pro Lys Glu Gly Glu Lys Asp Val Ala Arg Gly
115 120 125
Cys Gly Gln Gly Pro Ile Lys Cys Pro Gln Met Ile Ser Thr Leu Ala
130 135 140
Ser Cys Ser Leu Gln Glu Ile Val Glu Ala Ala Pro Ser Lys Glu Gln
145 150 155 160
Ile Gln Trp Phe Gln Leu Tyr Val Asn Thr Asp Arg Lys Ile Thr Asp
165 170 175
Asp Leu Ile Lys Gln Val Glu Lys Leu Gly Leu Lys Ala Ile Phe Val
180 185 190
Thr Val Asp Ala Pro Ser Leu Gly Arg Arg Glu Lys Asp Met Lys Val
195 200 205
Lys Phe Thr Asn Ser Asn Gly Ala Lys Ala Met Glu Lys Thr Lys Val
210 215 220
Lys Glu Ser Lys Gly Ala Ser Ala Ala Leu Ser Thr Phe Ile Asp Pro
225 230 235 240
Ala Leu Ser Trp Asp Asp Ile Val Glu Ile Lys Lys Lys Thr Asn Leu
245 250 255
Pro Ile Val Ile Lys Gly Val Gln Arg Val Glu Asp Val Leu Lys Ala
260 265 270
Ala Glu Val Gly Ala Ala Gly Val Val Leu Ser Asn His Gly Gly Arg
275 280 285
Gln Leu Asp Phe Ser Arg Ala Pro Ile Glu Val Leu Ala Glu Ser Val
290 295 300
Pro Val Leu Lys Glu Arg Asn Leu Ser Asp Lys Leu Glu Ile Phe Ile
305 310 315 320
Asp Gly Gly Val Arg Arg Gly Asn Asp Val Leu Lys Ala Leu Cys Leu
325 330 335
Gly Ala Lys Gly Val Gly Leu Gly Arg Pro Phe Leu Tyr Ala Asn Ser
340 345 350
Cys Tyr Gly Lys Asp Gly Val Gln Lys Ala Ile Glu Met Leu Thr Glu
355 360 365
Glu Ile Gln Met Ser Met Arg Leu Leu Gly Val Thr Lys Ile Glu Asp
370 375 380
Leu Lys Pro Glu Leu Leu Asp Met Ser Ser Phe Ser Gly Arg Thr Val
385 390 395 400
Asn Val Pro Gln Asp Ser Leu Tyr Arg Ser Val Tyr Glu Lys Ser Glu
405 410 415
Thr Val Gln Phe Leu Asp Glu
420
<210> 55
<211> 4704
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和贝酵母。
<400> 55
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgttaaaat acaaatctct 1560
gctgagaatc tcgagagtct ccgaggcagc cgctctcaga acgtccagga ctagattaaa 1620
cacagtccgc tcttacagtt ctgctgtggg caagtctaag tcgttcgaga aacattccgg 1680
aaaacgcaca cagtcatgga ctgctttgag cgtcggtgcc atcttagccg ctgctggttc 1740
aatggcttat gtaaactggg gtagtggcca gataggtaac gacccaaaac tagacatgaa 1800
taagcagaaa atatcgcctg cagaagtcgc taaacacaac tcggctgacg attgctgggt 1860
cgtgatcaac ggttatgtgt atgatctgac gcggttcgta cctaaccatc caggtggccc 1920
ggatgttatc aaatacaatg ctgggagaga cgtcactgcc atttttgagc cattgcatgc 1980
ccccaacgtc atcgataaat acattgctcc tgagaagaag ctgggaccgc tacaggggtc 2040
catgccacca gaactggttt gcccgccata cgctccaggc gaaaccaaag aagacattgc 2100
taggaaagag caactaaaat cattactccc cccactggat aacattatta atctttatga 2160
tttcgagtac ttggcttctc aaactttgac taggcaagcc tgggcttact actcctctgg 2220
ttccaatgac gaagtctcgc acagggaaaa ccataatgca taccacagga ttttcttcaa 2280
gccaaagatt cttgttgacg tgagcaaagt ggacatctcg acggacatgc tgggttctca 2340
tgtcgacgtc ccattctacg tgtctgcaac agccctgtgt aaactgggga acccactaga 2400
aggtgaaaag gacattgcta gaggctgtgg ccaaggcgca acgaaagtcc cacaaatgat 2460
ctccactttg gcgtcatgct caccagaaga aatcatcagt gcggcgccat ccgatgagca 2520
gattcaatgg tatcaactat acgttaactc tgatagaaag atcactgacg aattggttaa 2580
gagtgttgaa aaattgggcg tgaaggcact gtttgttact gtagatgcac caagtttagg 2640
ccagagagag aaggatatga agttaaaatt ctccaattca aaagctggcc caaaggcaat 2700
gaagaaaaca gatgtagagg aatctaaagg cgcctcaaga gcgttgtcca agtttattga 2760
cccctctttg acttggaagg atattgaaga gttgaaatca aagacaaacc tacccattgt 2820
catcaaaggt gttcaacgca ccgaagatgt cgttaaagca gctgaggttg gtgtaagtgg 2880
cgtggtgcta tccaaccacg gtggtagaca attagacttt tcgagagccc ccatcgaagt 2940
cttggctgaa gccatgccag ttctggaaga acgtaaattg aaggataaac tggaagtata 3000
catagacggt ggcgttcgtc gtggtacaga tatcctaaag gcgctgtgtc tgggcgctaa 3060
aggtgttggt ttgggtagac cattcctgta cgccaactca tgctatggtc gcgacggtgt 3120
tgagaaagcc attgaaattt tgcgagatga agtcgaaatg tccatgagac tcctgggtgt 3180
tgccagtgtt gcggaattga agcctgagct gttagatctg tcaacattga aggcaagaac 3240
agtggcagtg cccaacgatg tcttgtataa tgaggtttac gaaggcccca ctttaacaga 3300
ttttgaggac gcatgattat taattaaaca ggcccctttt cctttgtcga tatcatgtaa 3360
ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa 3420
ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta 3480
agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt 3540
aacgggcaga cggccggcca taacttcgta taatgtatgc tatacgaagt tatggcaacg 3600
gttcatcatc tcatggatct gcacatgaac aaacaccaga gtcaaacgac gttgaaattg 3660
aggctactgc gccaattgat gacaatacag acgatgataa caaaccgaag ttatctgatg 3720
tagaaaagga ttagagatgc taagagatag tgatgatatt tcataaataa tgtaattcta 3780
tatatgttaa ttaccttttt tgcgaggcat atttatggtg aaggataagt tttgaccatc 3840
aaagaaggtt aatgtggctg tggtttcagg gtccataaag cttttcaatt catctttttt 3900
ttttttgttc ttttttttga ttccggtttc tttgaaattt ttttgattcg gtaatctccg 3960
agcagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgtg 4020
gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct 4080
gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc 4140
ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg 4200
cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca 4260
aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag 4320
ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat 4380
ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag 4440
aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga 4500
agcaggcggc ggaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt 4560
catgcaaggg ctccctagct actggagaat atactaaggg tactgttgac attgcgaaga 4620
gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag 4680
gttacgattg gttgattatg acac 4704
<210> 56
<211> 591
<212> PRT
<213> 贝酵母(Saccharomyces bayanus)
<400> 56
Met Leu Lys Tyr Lys Ser Leu Leu Arg Ile Ser Arg Val Ser Glu Ala
1 5 10 15
Ala Ala Leu Arg Thr Ser Arg Thr Arg Leu Asn Thr Val Arg Ser Tyr
20 25 30
Ser Ser Ala Val Gly Lys Ser Lys Ser Phe Glu Lys His Ser Gly Lys
35 40 45
Arg Thr Gln Ser Trp Thr Ala Leu Ser Val Gly Ala Ile Leu Ala Ala
50 55 60
Ala Gly Ser Met Ala Tyr Val Asn Trp Gly Ser Gly Gln Ile Gly Asn
65 70 75 80
Asp Pro Lys Leu Asp Met Asn Lys Gln Lys Ile Ser Pro Ala Glu Val
85 90 95
Ala Lys His Asn Ser Ala Asp Asp Cys Trp Val Val Ile Asn Gly Tyr
100 105 110
Val Tyr Asp Leu Thr Arg Phe Val Pro Asn His Pro Gly Gly Pro Asp
115 120 125
Val Ile Lys Tyr Asn Ala Gly Arg Asp Val Thr Ala Ile Phe Glu Pro
130 135 140
Leu His Ala Pro Asn Val Ile Asp Lys Tyr Ile Ala Pro Glu Lys Lys
145 150 155 160
Leu Gly Pro Leu Gln Gly Ser Met Pro Pro Glu Leu Val Cys Pro Pro
165 170 175
Tyr Ala Pro Gly Glu Thr Lys Glu Asp Ile Ala Arg Lys Glu Gln Leu
180 185 190
Lys Ser Leu Leu Pro Pro Leu Asp Asn Ile Ile Asn Leu Tyr Asp Phe
195 200 205
Glu Tyr Leu Ala Ser Gln Thr Leu Thr Arg Gln Ala Trp Ala Tyr Tyr
210 215 220
Ser Ser Gly Ser Asn Asp Glu Val Ser His Arg Glu Asn His Asn Ala
225 230 235 240
Tyr His Arg Ile Phe Phe Lys Pro Lys Ile Leu Val Asp Val Ser Lys
245 250 255
Val Asp Ile Ser Thr Asp Met Leu Gly Ser His Val Asp Val Pro Phe
260 265 270
Tyr Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Leu Glu Gly
275 280 285
Glu Lys Asp Ile Ala Arg Gly Cys Gly Gln Gly Ala Thr Lys Val Pro
290 295 300
Gln Met Ile Ser Thr Leu Ala Ser Cys Ser Pro Glu Glu Ile Ile Ser
305 310 315 320
Ala Ala Pro Ser Asp Glu Gln Ile Gln Trp Tyr Gln Leu Tyr Val Asn
325 330 335
Ser Asp Arg Lys Ile Thr Asp Glu Leu Val Lys Ser Val Glu Lys Leu
340 345 350
Gly Val Lys Ala Leu Phe Val Thr Val Asp Ala Pro Ser Leu Gly Gln
355 360 365
Arg Glu Lys Asp Met Lys Leu Lys Phe Ser Asn Ser Lys Ala Gly Pro
370 375 380
Lys Ala Met Lys Lys Thr Asp Val Glu Glu Ser Lys Gly Ala Ser Arg
385 390 395 400
Ala Leu Ser Lys Phe Ile Asp Pro Ser Leu Thr Trp Lys Asp Ile Glu
405 410 415
Glu Leu Lys Ser Lys Thr Asn Leu Pro Ile Val Ile Lys Gly Val Gln
420 425 430
Arg Thr Glu Asp Val Val Lys Ala Ala Glu Val Gly Val Ser Gly Val
435 440 445
Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala Pro
450 455 460
Ile Glu Val Leu Ala Glu Ala Met Pro Val Leu Glu Glu Arg Lys Leu
465 470 475 480
Lys Asp Lys Leu Glu Val Tyr Ile Asp Gly Gly Val Arg Arg Gly Thr
485 490 495
Asp Ile Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly
500 505 510
Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly Arg Asp Gly Val Glu
515 520 525
Lys Ala Ile Glu Ile Leu Arg Asp Glu Val Glu Met Ser Met Arg Leu
530 535 540
Leu Gly Val Ala Ser Val Ala Glu Leu Lys Pro Glu Leu Leu Asp Leu
545 550 555 560
Ser Thr Leu Lys Ala Arg Thr Val Ala Val Pro Asn Asp Val Leu Tyr
565 570 575
Asn Glu Val Tyr Glu Gly Pro Thr Leu Thr Asp Phe Glu Asp Ala
580 585 590
<210> 57
<211> 4722
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和鲁氏接合酵母。
<400> 57
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgtggttta gatcgaagcc 1560
tatttggaag ttatcagagg ttactgctag atctttacgt ggtatcaata ctaagagatc 1620
ttttacctgg aattctaagg gcagcaatgc attcgtacgc tcctctattt tgaagaagcg 1680
tagtatggct actgcgttat tggttaccgc tgtagccgct gcatcgagtg ttaaattgtc 1740
ctctcgacct ttagataacg gaccgaaagt agatatgtca aaacctgcca ttagtccaga 1800
tgaagtcgct aaacacaatt cgcccgatga ttgttgggtt gttattgatg gatatgtcta 1860
cgatttgacg gaatttgctc ctgtacatcc tggtggacct acagtaatta aatcaaacgc 1920
aggtaaagat gtcagtgcaa tttttgatcc attgcatgct ccagatgtta ttgagaaatt 1980
tattgatcca agtaaaaggt taggtccctt ggagggcgat atgccaaagg aattggtttg 2040
tgcgcctttt acacccggtg aaacccctga tgatgttcaa agaaaattga aattaagagc 2100
taggttacct ccgctggatg caattaccaa tctgtacgat tttgaattct tagcatctca 2160
agttttaacc aagcaggcat gggcttacta ttcaagtggt gctgatgatg aaattaccat 2220
gagagaaaac cactttgcat accatagaat tttctttaag cctaaagttt tggtgaacgt 2280
tgctgaggtg gatacaaaga ctgaaatgct gggtgcacct gttgatgtgc cattctatgt 2340
tactgctaca gcattgtgta aactgggtaa tccagctgag ggtgaaaaag atattgcaag 2400
aggttgtgga tctggtgaga aaaaggtgcc tcaaatggtt tccacattag catcctgttc 2460
cttagaagaa gtggttaatg caggcaaaga agatcaaata agatggtttc agttgtacat 2520
gaatgaggat cgtagtgtag tggatcaaat gatctccagt gcagaaaaat tgggttacaa 2580
aggtatattc gtaactgtcg atgcacctgg gcttggtaat agagaaaagg atacaaaggt 2640
taaattttct agtcaggccg gtccgttgag tgtgaaaaag aaggaaaagg aggataaagg 2700
taaagataat ggagaatcta gtggagcctc taaatattta tctaaattta ttgatccttc 2760
attcgactgg gatgacttgg ttgaagtgaa gaaaaagaca aaattaccaa ttgtgattaa 2820
aggtgttcaa agagttgaag atgttgttaa ggctgctgaa gttggcgcta gtggtgttgt 2880
gctttcaaat catggtggta ggcaattaga tttctctagg tcaccaattg aagttttggc 2940
tgaagctcaa ccaattctca aagaacgcaa ttttgaaaac tttgatgttt tcgttgatgg 3000
tggtattcgc agaggtactg atgtggttaa ggctctttgt cttggtgcta aaggtgttgg 3060
tttaggtagg cctttcctat atgccaactc ggtttatggg aaggaaggtg ttcaaaaagc 3120
aattgatatt ctcaattttg aagtggagat gacgatgaga ttgcttggtg ttacttctat 3180
caagcaattg gggccggagt tgatagatac ttcatgtatt aaatccagat ctgttcaagt 3240
accaagggac tacctctacg acaagactta catgagttct gatttggtta gtttcatgcc 3300
accacaagaa gacgaggctg atggcgagaa cgagtgatta attaaacagg ccccttttcc 3360
tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc 3420
gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt 3480
atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac 3540
aaacgcgtgt acgcatgtaa cgggcagacg gccggccata acttcgtata atgtatgcta 3600
tacgaagtta tggcaacggt tcatcatctc atggatctgc acatgaacaa acaccagagt 3660
caaacgacgt tgaaattgag gctactgcgc caattgatga caatacagac gatgataaca 3720
aaccgaagtt atctgatgta gaaaaggatt agagatgcta agagatagtg atgatatttc 3780
ataaataatg taattctata tatgttaatt accttttttg cgaggcatat ttatggtgaa 3840
ggataagttt tgaccatcaa agaaggttaa tgtggctgtg gtttcagggt ccataaagct 3900
tttcaattca tctttttttt ttttgttctt ttttttgatt ccggtttctt tgaaattttt 3960
ttgattcggt aatctccgag cagaaggaag aacgaaggaa ggagcacaga cttagattgg 4020
tatatatacg catatgtggt gttgaagaaa catgaaattg cccagtattc ttaacccaac 4080
tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg 4140
aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa 4200
agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag 4260
ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt 4320
tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac 4380
tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg 4440
gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag 4500
gtattgttag cggtttgaag caggcggcgg aagaagtaac aaaggaacct agaggccttt 4560
tgatgttagc agaattgtca tgcaagggct ccctagctac tggagaatat actaagggta 4620
ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca 4680
tgggtggaag agatgaaggt tacgattggt tgattatgac ac 4722
<210> 58
<211> 598
<212> PRT
<213> 鲁氏接合酵母(Zygosaccharomyces rouxii)
<400> 58
Met Trp Phe Arg Ser Lys Pro Ile Trp Lys Leu Ser Glu Val Thr Ala
1 5 10 15
Arg Ser Leu Arg Gly Ile Asn Thr Lys Arg Ser Phe Thr Trp Asn Ser
20 25 30
Lys Gly Ser Asn Ala Phe Val Arg Ser Ser Ile Leu Lys Lys Arg Ser
35 40 45
Met Ala Thr Ala Leu Leu Val Thr Ala Val Ala Ala Ala Ser Ser Val
50 55 60
Lys Leu Ser Ser Arg Pro Leu Asp Asn Gly Pro Lys Val Asp Met Ser
65 70 75 80
Lys Pro Ala Ile Ser Pro Asp Glu Val Ala Lys His Asn Ser Pro Asp
85 90 95
Asp Cys Trp Val Val Ile Asp Gly Tyr Val Tyr Asp Leu Thr Glu Phe
100 105 110
Ala Pro Val His Pro Gly Gly Pro Thr Val Ile Lys Ser Asn Ala Gly
115 120 125
Lys Asp Val Ser Ala Ile Phe Asp Pro Leu His Ala Pro Asp Val Ile
130 135 140
Glu Lys Phe Ile Asp Pro Ser Lys Arg Leu Gly Pro Leu Glu Gly Asp
145 150 155 160
Met Pro Lys Glu Leu Val Cys Ala Pro Phe Thr Pro Gly Glu Thr Pro
165 170 175
Asp Asp Val Gln Arg Lys Leu Lys Leu Arg Ala Arg Leu Pro Pro Leu
180 185 190
Asp Ala Ile Thr Asn Leu Tyr Asp Phe Glu Phe Leu Ala Ser Gln Val
195 200 205
Leu Thr Lys Gln Ala Trp Ala Tyr Tyr Ser Ser Gly Ala Asp Asp Glu
210 215 220
Ile Thr Met Arg Glu Asn His Phe Ala Tyr His Arg Ile Phe Phe Lys
225 230 235 240
Pro Lys Val Leu Val Asn Val Ala Glu Val Asp Thr Lys Thr Glu Met
245 250 255
Leu Gly Ala Pro Val Asp Val Pro Phe Tyr Val Thr Ala Thr Ala Leu
260 265 270
Cys Lys Leu Gly Asn Pro Ala Glu Gly Glu Lys Asp Ile Ala Arg Gly
275 280 285
Cys Gly Ser Gly Glu Lys Lys Val Pro Gln Met Val Ser Thr Leu Ala
290 295 300
Ser Cys Ser Leu Glu Glu Val Val Asn Ala Gly Lys Glu Asp Gln Ile
305 310 315 320
Arg Trp Phe Gln Leu Tyr Met Asn Glu Asp Arg Ser Val Val Asp Gln
325 330 335
Met Ile Ser Ser Ala Glu Lys Leu Gly Tyr Lys Gly Ile Phe Val Thr
340 345 350
Val Asp Ala Pro Gly Leu Gly Asn Arg Glu Lys Asp Thr Lys Val Lys
355 360 365
Phe Ser Ser Gln Ala Gly Pro Leu Ser Val Lys Lys Lys Glu Lys Glu
370 375 380
Asp Lys Gly Lys Asp Asn Gly Glu Ser Ser Gly Ala Ser Lys Tyr Leu
385 390 395 400
Ser Lys Phe Ile Asp Pro Ser Phe Asp Trp Asp Asp Leu Val Glu Val
405 410 415
Lys Lys Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val Gln Arg Val
420 425 430
Glu Asp Val Val Lys Ala Ala Glu Val Gly Ala Ser Gly Val Val Leu
435 440 445
Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ser Pro Ile Glu
450 455 460
Val Leu Ala Glu Ala Gln Pro Ile Leu Lys Glu Arg Asn Phe Glu Asn
465 470 475 480
Phe Asp Val Phe Val Asp Gly Gly Ile Arg Arg Gly Thr Asp Val Val
485 490 495
Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly Arg Pro Phe
500 505 510
Leu Tyr Ala Asn Ser Val Tyr Gly Lys Glu Gly Val Gln Lys Ala Ile
515 520 525
Asp Ile Leu Asn Phe Glu Val Glu Met Thr Met Arg Leu Leu Gly Val
530 535 540
Thr Ser Ile Lys Gln Leu Gly Pro Glu Leu Ile Asp Thr Ser Cys Ile
545 550 555 560
Lys Ser Arg Ser Val Gln Val Pro Arg Asp Tyr Leu Tyr Asp Lys Thr
565 570 575
Tyr Met Ser Ser Asp Leu Val Ser Phe Met Pro Pro Gln Glu Asp Glu
580 585 590
Ala Asp Gly Glu Asn Glu
595
<210> 59
<211> 4695
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和乳酸克鲁维酵母。
<400> 59
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgagatcag ccgtaagagt 1560
ttttggtaag aaccgtgtta gtgggttgag atttgctagc accagtagta aatctccaat 1620
tcataagtct agtgctactc tgcgttttgg gagatcagct aagtctggct atagtgcgtt 1680
ttcgttcggt aagaaagtca tcactggtgg tgcctttctt gttgccggtt cggttggtat 1740
tgcattgatt tctcaattca atgatagcgt tgaaaatggt gccaaggtcg atatgacgaa 1800
accaaaggtt tctccaacag aagtcgcaaa gcactcttct cccaaagact gttgggtggt 1860
cattgagggt tacgtataca atttgactga tttcatcagc gcccatcctg gtggtccagc 1920
tatcattgag aacaacgcag gaaaagatgt gaccaagatc tttgctccaa ttcatgctcc 1980
agatgtcatc gaaaaataca ttgctccaga gaacagaatt ggtccactag atggtacaat 2040
gcctgatgac ttggtctgtg ctgcattgac cccaggtgaa actcccgaag atgtggcaag 2100
aaaggaacaa ttgcgtcaat ctttacccga tattgattcc ttggttaaca tctatgattt 2160
cgaattcttg gcatcccaaa tcttgactaa gcaagcttgg tcttactact cttctgccgc 2220
tgatgatgaa gttacacata gagaaaacca cgctgcatac catcgtatat tcttcaagcc 2280
aagaatcttg gttaacgtta aggaagttga cacttccact acaatgttag gtgaaaaagt 2340
cggtgttcca ttttatgtgt ctgccaccgc attgtgtaaa ctaggtaacc ccaaggaagg 2400
tgaaaaggac atcgcaagag gttgcggtga aagtgacgtg aaacctgttc aaatgatttc 2460
cactttggct tcttgttctt tacaagaaat cgtggaagca gccccatcta aggaacaaat 2520
ccaatggttc caattgtatg tgaacagtga ccgtaagatc actgaagatc taatcaagaa 2580
cgttgaaaaa ttaggattaa aggccatttt cgttacagta gatgctcctt ccttaggtaa 2640
cagagaaaaa gatgctaaag tcaaatttac aaactcaaac ggtgccaagg ctatggaaaa 2700
aagtaaggtt aaggaatcta agggtgcttc tagagctctc tcgtcattca ttgatcctgc 2760
attaaactgg gatgatatca tcgaatttaa gaaaaagacc aaattgccaa tcgtcatcaa 2820
gggtgttcaa tgtgttgaag atgtcttgaa agctgctgaa atcggtgttg caggtgtcgt 2880
tctatctaac cacggtggta gacaattgga tttctctagg gctcctatcg aagtgttggc 2940
tgagactatg ccagtgctaa gagaaaagaa attggatgat aaaatcgaaa ttttcattga 3000
tggtggtgtt agaagaggta ccgatatcct aaaggcattg tgtcttggtg ccaagggtgt 3060
cggtctaggt agaccattct tatactctaa cagttgctac ggtaaagaag gtgtcaagaa 3120
agccattgag ctattaaagg acgaattgga aatgtcgatg agattactcg gtgtcacaag 3180
tatagaccaa ttatccgaaa aatacctaga tctatctaca atccatggta gaactgtcaa 3240
tgttcctcgc gataacctat accgtgaggt atatgtccca catgaaccaa ctcaattctt 3300
agaggaatga ttaattaaac aggccccttt tcctttgtcg atatcatgta attagttatg 3360
tcacgcttac attcacgccc tcctcccaca tccgctctaa ccgaaaagga aggagttaga 3420
caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 3480
tttatatttc aaatttttct tttttttctg tacaaacgcg tgtacgcatg taacgggcag 3540
acggccggcc ataacttcgt ataatgtatg ctatacgaag ttatggcaac ggttcatcat 3600
ctcatggatc tgcacatgaa caaacaccag agtcaaacga cgttgaaatt gaggctactg 3660
cgccaattga tgacaataca gacgatgata acaaaccgaa gttatctgat gtagaaaagg 3720
attagagatg ctaagagata gtgatgatat ttcataaata atgtaattct atatatgtta 3780
attacctttt ttgcgaggca tatttatggt gaaggataag ttttgaccat caaagaaggt 3840
taatgtggct gtggtttcag ggtccataaa gcttttcaat tcatcttttt tttttttgtt 3900
cttttttttg attccggttt ctttgaaatt tttttgattc ggtaatctcc gagcagaagg 3960
aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt ggtgttgaag 4020
aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac 4080
gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg 4140
ttgctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg 4200
atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt 4260
tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc 4320
taaaggcatt atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca 4380
ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag 4440
acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg 4500
cggaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg 4560
gctccctagc tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag 4620
attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt 4680
ggttgattat gacac 4695
<210> 60
<211> 589
<212> PRT
<213> 乳酸克鲁维酵母(Kluyveromyces lactis)
<400> 60
Met Arg Ser Ala Val Arg Val Phe Gly Lys Asn Arg Val Ser Gly Leu
1 5 10 15
Arg Phe Ala Ser Thr Ser Ser Lys Ser Pro Ile His Lys Ser Ser Ala
20 25 30
Thr Leu Arg Phe Gly Arg Ser Ala Lys Ser Gly Tyr Ser Ala Phe Ser
35 40 45
Phe Gly Lys Lys Val Ile Thr Gly Gly Ala Phe Leu Val Ala Gly Ser
50 55 60
Val Gly Ile Ala Leu Ile Ser Gln Phe Asn Asp Ser Val Glu Asn Gly
65 70 75 80
Ala Lys Val Asp Met Thr Lys Pro Lys Val Ser Pro Thr Glu Val Ala
85 90 95
Lys His Ser Ser Pro Lys Asp Cys Trp Val Val Ile Glu Gly Tyr Val
100 105 110
Tyr Asn Leu Thr Asp Phe Ile Ser Ala His Pro Gly Gly Pro Ala Ile
115 120 125
Ile Glu Asn Asn Ala Gly Lys Asp Val Thr Lys Ile Phe Ala Pro Ile
130 135 140
His Ala Pro Asp Val Ile Glu Lys Tyr Ile Ala Pro Glu Asn Arg Ile
145 150 155 160
Gly Pro Leu Asp Gly Thr Met Pro Asp Asp Leu Val Cys Ala Ala Leu
165 170 175
Thr Pro Gly Glu Thr Pro Glu Asp Val Ala Arg Lys Glu Gln Leu Arg
180 185 190
Gln Ser Leu Pro Asp Ile Asp Ser Leu Val Asn Ile Tyr Asp Phe Glu
195 200 205
Phe Leu Ala Ser Gln Ile Leu Thr Lys Gln Ala Trp Ser Tyr Tyr Ser
210 215 220
Ser Ala Ala Asp Asp Glu Val Thr His Arg Glu Asn His Ala Ala Tyr
225 230 235 240
His Arg Ile Phe Phe Lys Pro Arg Ile Leu Val Asn Val Lys Glu Val
245 250 255
Asp Thr Ser Thr Thr Met Leu Gly Glu Lys Val Gly Val Pro Phe Tyr
260 265 270
Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Lys Glu Gly Glu
275 280 285
Lys Asp Ile Ala Arg Gly Cys Gly Glu Ser Asp Val Lys Pro Val Gln
290 295 300
Met Ile Ser Thr Leu Ala Ser Cys Ser Leu Gln Glu Ile Val Glu Ala
305 310 315 320
Ala Pro Ser Lys Glu Gln Ile Gln Trp Phe Gln Leu Tyr Val Asn Ser
325 330 335
Asp Arg Lys Ile Thr Glu Asp Leu Ile Lys Asn Val Glu Lys Leu Gly
340 345 350
Leu Lys Ala Ile Phe Val Thr Val Asp Ala Pro Ser Leu Gly Asn Arg
355 360 365
Glu Lys Asp Ala Lys Val Lys Phe Thr Asn Ser Asn Gly Ala Lys Ala
370 375 380
Met Glu Lys Ser Lys Val Lys Glu Ser Lys Gly Ala Ser Arg Ala Leu
385 390 395 400
Ser Ser Phe Ile Asp Pro Ala Leu Asn Trp Asp Asp Ile Ile Glu Phe
405 410 415
Lys Lys Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val Gln Cys Val
420 425 430
Glu Asp Val Leu Lys Ala Ala Glu Ile Gly Val Ala Gly Val Val Leu
435 440 445
Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala Pro Ile Glu
450 455 460
Val Leu Ala Glu Thr Met Pro Val Leu Arg Glu Lys Lys Leu Asp Asp
465 470 475 480
Lys Ile Glu Ile Phe Ile Asp Gly Gly Val Arg Arg Gly Thr Asp Ile
485 490 495
Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly Arg Pro
500 505 510
Phe Leu Tyr Ser Asn Ser Cys Tyr Gly Lys Glu Gly Val Lys Lys Ala
515 520 525
Ile Glu Leu Leu Lys Asp Glu Leu Glu Met Ser Met Arg Leu Leu Gly
530 535 540
Val Thr Ser Ile Asp Gln Leu Ser Glu Lys Tyr Leu Asp Leu Ser Thr
545 550 555 560
Ile His Gly Arg Thr Val Asn Val Pro Arg Asp Asn Leu Tyr Arg Glu
565 570 575
Val Tyr Val Pro His Glu Pro Thr Gln Phe Leu Glu Glu
580 585
<210> 61
<211> 4704
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和多布克鲁维酵母。
<400> 61
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgcgttctg cagttagagt 1560
ttttcgtaaa aacagatttc caacaggttt gagattgtca tcaacagcat cttccgctag 1620
acaaggtatc gctctttccg tagggaaata caacaagtac aacaaaaatg gtaccaacgg 1680
gtcttggaaa tcttccagaa aatcaaccac ctacggaggt gccttccttg tggcgagtgc 1740
agtcgcaatt gctttcagct ctcaaattac ccaatcctta gaaaatggtg ttaaggtgga 1800
tatggggaag ccatctgttt ctccagcgga agtagttaag cactcatcac cagatgattg 1860
ttgggttgtc atagatggtt acgtatacaa tctaactgaa ttcatcggcg cccatcctgg 1920
tggtccagcg ataatcaaaa acaacgcagg caaggacgtt actaagatat ttgcaccaat 1980
ccatgctcca gacgttattg aaaagtacat cgctcctaaa aacagactgg gtccattgga 2040
aggtacaatg cctgaggacc gtatttgcgc tgcactcaca ccaggtgaaa ctgccgagga 2100
tgtcgcgaga aaggaacaac tgagacagtt gttgcctgac gtcaacaact taactaacct 2160
ttatgatttc gaattcttag catcacagat tttgactaag caagcttggt cttactactc 2220
tagcgccgca gatgacgaag tgacacaaag agagaatcac gcagcatatc atagaatttt 2280
ctttaagcct agaatcttag ttaatgtcaa ggaggtagac acttccacaa caatgctggg 2340
agagaaggtt ggagtcccat tctacgtatc agctaccgcc ttgtgcaagt tgggtaaccc 2400
acaagagggt gaaaaagaca tagcaagagg atgtggtgaa tccgatgcta aacctgtgca 2460
aatgatttct acattggctt catgttcact acaggaaatc gttgaagctg ccccttcaaa 2520
ggaacaaata caatggtttc aactatatgt taacagcgat cgtaagatta ctgatgatct 2580
tgttaaaaac gctgaaaagc tcggtttaaa ggccattttt gtgactgttg atgccccttc 2640
tttaggaaat agagagaagg acgctaaggt taagttcaca aattctaacg gtgcaaaagc 2700
tatggaaaag tcaaatgtag atgaaagtaa gggcgcttcc agagcattat cttcattcat 2760
tgacccagcc ctaaattggg atgatatcgt ggaactcaag acaaaaacaa agttaccaat 2820
cgtcatcaaa ggagtgcaat gtgttgaaga tgtccttaaa gcagctgagg ttggagcggc 2880
cggtgtagtt ttgtctaacc atggtggcag acaattggat ttcagtagag caccaatcga 2940
agtgttagct gaaactatgc ctgcattgag agagaaaaag ctagatgata agttagaagt 3000
tttcattgat ggcggggtca gaagaggcac agatatccta aaggctttat gtctaggagc 3060
aaaaggcgtt ggactgggta gaccatttct ttactctaac agttgctacg gtaaagaggg 3120
ggtcaaaaag gccattgaat tgttgaagga tgagttggaa atgtctatga gattgcttgg 3180
cgtgacaagt attgaccagt tatcagaaaa gtacctcgat ctttcaacta tccacggtag 3240
aacagtttct gtcccaagag ataacattta cagagatgta tacgttgcac aagagccatc 3300
tcagttcaga gaggactagt taattaaaca ggcccctttt cctttgtcga tatcatgtaa 3360
ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa 3420
ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta 3480
agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt 3540
aacgggcaga cggccggcca taacttcgta taatgtatgc tatacgaagt tatggcaacg 3600
gttcatcatc tcatggatct gcacatgaac aaacaccaga gtcaaacgac gttgaaattg 3660
aggctactgc gccaattgat gacaatacag acgatgataa caaaccgaag ttatctgatg 3720
tagaaaagga ttagagatgc taagagatag tgatgatatt tcataaataa tgtaattcta 3780
tatatgttaa ttaccttttt tgcgaggcat atttatggtg aaggataagt tttgaccatc 3840
aaagaaggtt aatgtggctg tggtttcagg gtccataaag cttttcaatt catctttttt 3900
ttttttgttc ttttttttga ttccggtttc tttgaaattt ttttgattcg gtaatctccg 3960
agcagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgtg 4020
gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct 4080
gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc 4140
ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg 4200
cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca 4260
aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag 4320
ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat 4380
ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag 4440
aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga 4500
agcaggcggc ggaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt 4560
catgcaaggg ctccctagct actggagaat atactaaggg tactgttgac attgcgaaga 4620
gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag 4680
gttacgattg gttgattatg acac 4704
<210> 62
<211> 592
<212> PRT
<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)
<400> 62
Met Arg Ser Ala Val Arg Val Phe Arg Lys Asn Arg Phe Pro Thr Gly
1 5 10 15
Leu Arg Leu Ser Ser Thr Ala Ser Ser Ala Arg Gln Gly Ile Ala Leu
20 25 30
Ser Val Gly Lys Tyr Asn Lys Tyr Asn Lys Asn Gly Thr Asn Gly Ser
35 40 45
Trp Lys Ser Ser Arg Lys Ser Thr Thr Tyr Gly Gly Ala Phe Leu Val
50 55 60
Ala Ser Ala Val Ala Ile Ala Phe Ser Ser Gln Ile Thr Gln Ser Leu
65 70 75 80
Glu Asn Gly Val Lys Val Asp Met Gly Lys Pro Ser Val Ser Pro Ala
85 90 95
Glu Val Val Lys His Ser Ser Pro Asp Asp Cys Trp Val Val Ile Asp
100 105 110
Gly Tyr Val Tyr Asn Leu Thr Glu Phe Ile Gly Ala His Pro Gly Gly
115 120 125
Pro Ala Ile Ile Lys Asn Asn Ala Gly Lys Asp Val Thr Lys Ile Phe
130 135 140
Ala Pro Ile His Ala Pro Asp Val Ile Glu Lys Tyr Ile Ala Pro Lys
145 150 155 160
Asn Arg Leu Gly Pro Leu Glu Gly Thr Met Pro Glu Asp Arg Ile Cys
165 170 175
Ala Ala Leu Thr Pro Gly Glu Thr Ala Glu Asp Val Ala Arg Lys Glu
180 185 190
Gln Leu Arg Gln Leu Leu Pro Asp Val Asn Asn Leu Thr Asn Leu Tyr
195 200 205
Asp Phe Glu Phe Leu Ala Ser Gln Ile Leu Thr Lys Gln Ala Trp Ser
210 215 220
Tyr Tyr Ser Ser Ala Ala Asp Asp Glu Val Thr Gln Arg Glu Asn His
225 230 235 240
Ala Ala Tyr His Arg Ile Phe Phe Lys Pro Arg Ile Leu Val Asn Val
245 250 255
Lys Glu Val Asp Thr Ser Thr Thr Met Leu Gly Glu Lys Val Gly Val
260 265 270
Pro Phe Tyr Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Gln
275 280 285
Glu Gly Glu Lys Asp Ile Ala Arg Gly Cys Gly Glu Ser Asp Ala Lys
290 295 300
Pro Val Gln Met Ile Ser Thr Leu Ala Ser Cys Ser Leu Gln Glu Ile
305 310 315 320
Val Glu Ala Ala Pro Ser Lys Glu Gln Ile Gln Trp Phe Gln Leu Tyr
325 330 335
Val Asn Ser Asp Arg Lys Ile Thr Asp Asp Leu Val Lys Asn Ala Glu
340 345 350
Lys Leu Gly Leu Lys Ala Ile Phe Val Thr Val Asp Ala Pro Ser Leu
355 360 365
Gly Asn Arg Glu Lys Asp Ala Lys Val Lys Phe Thr Asn Ser Asn Gly
370 375 380
Ala Lys Ala Met Glu Lys Ser Asn Val Asp Glu Ser Lys Gly Ala Ser
385 390 395 400
Arg Ala Leu Ser Ser Phe Ile Asp Pro Ala Leu Asn Trp Asp Asp Ile
405 410 415
Val Glu Leu Lys Thr Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val
420 425 430
Gln Cys Val Glu Asp Val Leu Lys Ala Ala Glu Val Gly Ala Ala Gly
435 440 445
Val Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala
450 455 460
Pro Ile Glu Val Leu Ala Glu Thr Met Pro Ala Leu Arg Glu Lys Lys
465 470 475 480
Leu Asp Asp Lys Leu Glu Val Phe Ile Asp Gly Gly Val Arg Arg Gly
485 490 495
Thr Asp Ile Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu
500 505 510
Gly Arg Pro Phe Leu Tyr Ser Asn Ser Cys Tyr Gly Lys Glu Gly Val
515 520 525
Lys Lys Ala Ile Glu Leu Leu Lys Asp Glu Leu Glu Met Ser Met Arg
530 535 540
Leu Leu Gly Val Thr Ser Ile Asp Gln Leu Ser Glu Lys Tyr Leu Asp
545 550 555 560
Leu Ser Thr Ile His Gly Arg Thr Val Ser Val Pro Arg Asp Asn Ile
565 570 575
Tyr Arg Asp Val Tyr Val Ala Gln Glu Pro Ser Gln Phe Arg Glu Asp
580 585 590
<210> 63
<211> 4620
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和克鲁弗酵母。
<400> 63
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgtttaaaa gagccttttt 1560
gagacctcaa ttgtggtcct cgacgactcg tcgctgcaca atagctagca ttcggtgcta 1620
ctcgaaacaa ccgttaaaac cggcctcagc tagtaagttg actcttggtt tgatcggtgg 1680
tatcggcttg ggatctgctg ctacagcata cggactattg atgggggacg acgatacttc 1740
tgcctcgaca acgagactag atgaattagc gcctccaagc tattgccagg atcctaagaa 1800
gtttgaagac gcaattataa aactaaaaca cattcttgat gacaatccag acaacttttc 1860
tgtttctaaa agtgagcttg atgctcattc cgacacttac ttcaactctc accatgccag 1920
gcccgaccaa agaccacaga ttatcctatt tccacgtact acatcagatg tttccgagat 1980
tttgaaagtt tgccacgaat acgaggtgcc agttatccca ttttccggtg gtacttctct 2040
ggagggccat ttcttaccaa caagaattgg ctgcaccgta gtgttagaca tgtccaaata 2100
tatgaacaaa attttgaagc tcaacaagca ggacttggat gttgaggtcc aagccggtgt 2160
accatgggaa gatctaaacg attatttgaa ttctgaaggt gtcttatttg gttgcgatcc 2220
tggtccaggt gctcaaatcg gtggctgtat tgctaactcc tgttccggta ctaatgctta 2280
ccgttacggt acgatgaagg aaaacatagt gaacatcact gtggtcctag ctgatggtac 2340
tgttgtcaag actaagagac gtcctagaaa atcaagtgct ggttacaact tgaacgggtt 2400
gtttgttggt agcgagggta ctctcggtgt tgtcactcaa gctaccgtaa aatgtcatgt 2460
catgcccaag atcgaaactg tcgctgtcgt ttcctttcca actgtgggtg atgcagcatc 2520
ttgctcctct catatcatcc aagaaggtat acagctaaat gctatggaac tattggacga 2580
taatatgatg aaggtgatca accaatccgg tgccacatcc aagacggact gggtcgaatc 2640
tccaacgttg tttttcaaga ttggaggaag atctcaaaat aacgtcaaag aattggttga 2700
tgaagtttcc aagattgctc aaaaatacca ttgcaagaag tttgaattcg cctctagtga 2760
cgatgaaaag ttggaattat gggaagccag aaaggtcgcg ctatggtcta ccatcaatgc 2820
ggggaaagcc aaggacagta actccaacgt ttggaccaca gacgttgcag tcccactgtc 2880
tcagtttgca cctgtcatcg aagccaccaa gcaagagatg aacgccagcg gcttaattac 2940
cactctggtt ggccatgcgg gtgatggtaa tttccacgct ttcattgttt acaacgacga 3000
ccaacgtgac atcgccgaaa aaatcgtcga taacatggtc catagggcca tagaagcaga 3060
aggcacttgt accggtgagc acggcgttgg tatcggaaaa agaaaattct tgcttcatga 3120
gttaggtgct gaaacagttg atctaatgag acagttaaaa ttagccttgg atccaaagag 3180
aatattgaat ccagacaaga tcttcaagat tgatcctaac gagcatgaac actgattaat 3240
taaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca 3300
cgccctcctc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 3360
gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 3420
tttctttttt ttctgtacaa acgcgtgtac gcatgtaacg ggcagacggc cggccataac 3480
ttcgtataat gtatgctata cgaagttatg gcaacggttc atcatctcat ggatctgcac 3540
atgaacaaac accagagtca aacgacgttg aaattgaggc tactgcgcca attgatgaca 3600
atacagacga tgataacaaa ccgaagttat ctgatgtaga aaaggattag agatgctaag 3660
agatagtgat gatatttcat aaataatgta attctatata tgttaattac cttttttgcg 3720
aggcatattt atggtgaagg ataagttttg accatcaaag aaggttaatg tggctgtggt 3780
ttcagggtcc ataaagcttt tcaattcatc tttttttttt ttgttctttt ttttgattcc 3840
ggtttctttg aaattttttt gattcggtaa tctccgagca gaaggaagaa cgaaggaagg 3900
agcacagact tagattggta tatatacgca tatgtggtgt tgaagaaaca tgaaattgcc 3960
cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 4020
cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 4080
ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 4140
aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 4200
tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 4260
ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 4320
aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 4380
acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcggaa gaagtaacaa 4440
aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctagctactg 4500
gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 4560
ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 4620
<210> 64
<211> 564
<212> PRT
<213> 克鲁弗酵母(Saccharomyces kluyveri)
<400> 64
Met Phe Lys Arg Ala Phe Leu Arg Pro Gln Leu Trp Ser Ser Thr Thr
1 5 10 15
Arg Arg Cys Thr Ile Ala Ser Ile Arg Cys Tyr Ser Lys Gln Pro Leu
20 25 30
Lys Pro Ala Ser Ala Ser Lys Leu Thr Leu Gly Leu Ile Gly Gly Ile
35 40 45
Gly Leu Gly Ser Ala Ala Thr Ala Tyr Gly Leu Leu Met Gly Asp Asp
50 55 60
Asp Thr Ser Ala Ser Thr Thr Arg Leu Asp Glu Leu Ala Pro Pro Ser
65 70 75 80
Tyr Cys Gln Asp Pro Lys Lys Phe Glu Asp Ala Ile Ile Lys Leu Lys
85 90 95
His Ile Leu Asp Asp Asn Pro Asp Asn Phe Ser Val Ser Lys Ser Glu
100 105 110
Leu Asp Ala His Ser Asp Thr Tyr Phe Asn Ser His His Ala Arg Pro
115 120 125
Asp Gln Arg Pro Gln Ile Ile Leu Phe Pro Arg Thr Thr Ser Asp Val
130 135 140
Ser Glu Ile Leu Lys Val Cys His Glu Tyr Glu Val Pro Val Ile Pro
145 150 155 160
Phe Ser Gly Gly Thr Ser Leu Glu Gly His Phe Leu Pro Thr Arg Ile
165 170 175
Gly Cys Thr Val Val Leu Asp Met Ser Lys Tyr Met Asn Lys Ile Leu
180 185 190
Lys Leu Asn Lys Gln Asp Leu Asp Val Glu Val Gln Ala Gly Val Pro
195 200 205
Trp Glu Asp Leu Asn Asp Tyr Leu Asn Ser Glu Gly Val Leu Phe Gly
210 215 220
Cys Asp Pro Gly Pro Gly Ala Gln Ile Gly Gly Cys Ile Ala Asn Ser
225 230 235 240
Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu Asn Ile
245 250 255
Val Asn Ile Thr Val Val Leu Ala Asp Gly Thr Val Val Lys Thr Lys
260 265 270
Arg Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asn Leu Asn Gly Leu Phe
275 280 285
Val Gly Ser Glu Gly Thr Leu Gly Val Val Thr Gln Ala Thr Val Lys
290 295 300
Cys His Val Met Pro Lys Ile Glu Thr Val Ala Val Val Ser Phe Pro
305 310 315 320
Thr Val Gly Asp Ala Ala Ser Cys Ser Ser His Ile Ile Gln Glu Gly
325 330 335
Ile Gln Leu Asn Ala Met Glu Leu Leu Asp Asp Asn Met Met Lys Val
340 345 350
Ile Asn Gln Ser Gly Ala Thr Ser Lys Thr Asp Trp Val Glu Ser Pro
355 360 365
Thr Leu Phe Phe Lys Ile Gly Gly Arg Ser Gln Asn Asn Val Lys Glu
370 375 380
Leu Val Asp Glu Val Ser Lys Ile Ala Gln Lys Tyr His Cys Lys Lys
385 390 395 400
Phe Glu Phe Ala Ser Ser Asp Asp Glu Lys Leu Glu Leu Trp Glu Ala
405 410 415
Arg Lys Val Ala Leu Trp Ser Thr Ile Asn Ala Gly Lys Ala Lys Asp
420 425 430
Ser Asn Ser Asn Val Trp Thr Thr Asp Val Ala Val Pro Leu Ser Gln
435 440 445
Phe Ala Pro Val Ile Glu Ala Thr Lys Gln Glu Met Asn Ala Ser Gly
450 455 460
Leu Ile Thr Thr Leu Val Gly His Ala Gly Asp Gly Asn Phe His Ala
465 470 475 480
Phe Ile Val Tyr Asn Asp Asp Gln Arg Asp Ile Ala Glu Lys Ile Val
485 490 495
Asp Asn Met Val His Arg Ala Ile Glu Ala Glu Gly Thr Cys Thr Gly
500 505 510
Glu His Gly Val Gly Ile Gly Lys Arg Lys Phe Leu Leu His Glu Leu
515 520 525
Gly Ala Glu Thr Val Asp Leu Met Arg Gln Leu Lys Leu Ala Leu Asp
530 535 540
Pro Lys Arg Ile Leu Asn Pro Asp Lys Ile Phe Lys Ile Asp Pro Asn
545 550 555 560
Glu His Glu His
<210> 65
<211> 4521
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和贝酵母。
<400> 65
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgctgagaa acattttggt 1560
gagaggcgcc ggcaaacatc tgaaatatgg ttgcagatat gtgaaatcgt ctgctctttt 1620
ggggtgtagg acaaaaatca actactattc cactaagata caaacaaaac taaccagcga 1680
aaactaccca gacgtgcaaa gagatgcgag attcaagaaa ctgacttctg atgatttgaa 1740
ctatttcagg tccattttgt cagaacagga ggtattacag gccagcgaat tagaggacct 1800
gtcattttat aatgaagact ggatgagaaa atataaaggc cagtccaagt tagttttgag 1860
gcctaaaacc gtggaaaaga tttcactgat tttgaattat tgtaatgatg aaaaaattgc 1920
tgttgtacca caaggtggta acactggatt ggtaggtggt tcggtaccta ttttcgatga 1980
gttgattcta tcattagcca atttgaacaa aataagagat ttcgaccctg tgtcaggtat 2040
tttaaagtgt gatgccggtg ttatcttgga aaatgctaat aattacgtaa tggagcaaaa 2100
ttatatgttc ccgttggatt taggtgctaa aggttcttgc catgttggtg gggtggttgc 2160
caccaacgca ggtggattga gattattgcg ttatggctca ctacatggca gcgtgttagg 2220
tttggaagta gtcatgccta atggtcaaat cgttaatagt atgcattcga tgagaaagga 2280
caacaccggt tatgacttaa aacaactatt catcggctct gaaggtacta ttggtatcat 2340
tactggtgtt tcggtcttga cggttcctaa gccaaaggct ttcaatgttt cctatttagc 2400
tgttgagagt tttgaagatg ttcaaaaagt attcgtcaga gccagacagg agttgtctga 2460
aattctatca gcttttgagt tcatggattc caaatctcaa atcttggcca aaagtcagtt 2520
gaaagatgcc actttcccct tagaagacga acatccattt tatattctta tcgagacatc 2580
gggatcaaac aaggatcacg atgattctaa acttgaaaca ttcttagaaa gcgtcatgga 2640
agaaggcgta gtaacagatg gtgtggtagc acaagacgaa actgagttgc aaaacttatg 2700
gaagtggaga gaaatgattc cagaggcaag ccaagctaat ggtggtgttt acaaatacga 2760
tgtttctctt ccattaaagg atctatactc cctggttgat gccaccaatg ccaggctatc 2820
tgaagcagga ctggtcggag attcacccaa acccgtcgtg ggtgccattg ggtatggcca 2880
tgtgggggat ggtaatctac atttaaacgt tgctgttaga gaatacaaca aggaaattga 2940
aaaggtttta gaaccatttg tttatgaatt cgtttcctca aagcatggtt ccgtgagtgc 3000
tgagcatggt ttgggtttcc agaagaaaaa ttacattggt tattccaaga gtcctgaaga 3060
agttacaatg ataaaggatc tgaaagcaca ttacgacccc aatgcaatct tgaaccctta 3120
caaatacgtt tgaaaattaa ttaaacaggc cccttttcct ttgtcgatat catgtaatta 3180
gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga 3240
gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 3300
acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac 3360
gggcagacgg ccggccataa cttcgtataa tgtatgctat acgaagttat ggcaacggtt 3420
catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt gaaattgagg 3480
ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta tctgatgtag 3540
aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt aattctatat 3600
atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa 3660
gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat cttttttttt 3720
tttgttcttt tttttgattc cggtttcttt gaaatttttt tgattcggta atctccgagc 3780
agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtggtg 3840
ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca 3900
ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta 3960
gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt 4020
cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa 4080
tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta 4140
agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg 4200
ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat 4260
gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc 4320
aggcggcgga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat 4380
gcaagggctc cctagctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg 4440
acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt 4500
acgattggtt gattatgaca c 4521
<210> 66
<211> 530
<212> PRT
<213> 贝酵母(Saccharomyces bayanus)
<400> 66
Met Leu Arg Asn Ile Leu Val Arg Gly Ala Gly Lys His Leu Lys Tyr
1 5 10 15
Gly Cys Arg Tyr Val Lys Ser Ser Ala Leu Leu Gly Cys Arg Thr Lys
20 25 30
Ile Asn Tyr Tyr Ser Thr Lys Ile Gln Thr Lys Leu Thr Ser Glu Asn
35 40 45
Tyr Pro Asp Val Gln Arg Asp Ala Arg Phe Lys Lys Leu Thr Ser Asp
50 55 60
Asp Leu Asn Tyr Phe Arg Ser Ile Leu Ser Glu Gln Glu Val Leu Gln
65 70 75 80
Ala Ser Glu Leu Glu Asp Leu Ser Phe Tyr Asn Glu Asp Trp Met Arg
85 90 95
Lys Tyr Lys Gly Gln Ser Lys Leu Val Leu Arg Pro Lys Thr Val Glu
100 105 110
Lys Ile Ser Leu Ile Leu Asn Tyr Cys Asn Asp Glu Lys Ile Ala Val
115 120 125
Val Pro Gln Gly Gly Asn Thr Gly Leu Val Gly Gly Ser Val Pro Ile
130 135 140
Phe Asp Glu Leu Ile Leu Ser Leu Ala Asn Leu Asn Lys Ile Arg Asp
145 150 155 160
Phe Asp Pro Val Ser Gly Ile Leu Lys Cys Asp Ala Gly Val Ile Leu
165 170 175
Glu Asn Ala Asn Asn Tyr Val Met Glu Gln Asn Tyr Met Phe Pro Leu
180 185 190
Asp Leu Gly Ala Lys Gly Ser Cys His Val Gly Gly Val Val Ala Thr
195 200 205
Asn Ala Gly Gly Leu Arg Leu Leu Arg Tyr Gly Ser Leu His Gly Ser
210 215 220
Val Leu Gly Leu Glu Val Val Met Pro Asn Gly Gln Ile Val Asn Ser
225 230 235 240
Met His Ser Met Arg Lys Asp Asn Thr Gly Tyr Asp Leu Lys Gln Leu
245 250 255
Phe Ile Gly Ser Glu Gly Thr Ile Gly Ile Ile Thr Gly Val Ser Val
260 265 270
Leu Thr Val Pro Lys Pro Lys Ala Phe Asn Val Ser Tyr Leu Ala Val
275 280 285
Glu Ser Phe Glu Asp Val Gln Lys Val Phe Val Arg Ala Arg Gln Glu
290 295 300
Leu Ser Glu Ile Leu Ser Ala Phe Glu Phe Met Asp Ser Lys Ser Gln
305 310 315 320
Ile Leu Ala Lys Ser Gln Leu Lys Asp Ala Thr Phe Pro Leu Glu Asp
325 330 335
Glu His Pro Phe Tyr Ile Leu Ile Glu Thr Ser Gly Ser Asn Lys Asp
340 345 350
His Asp Asp Ser Lys Leu Glu Thr Phe Leu Glu Ser Val Met Glu Glu
355 360 365
Gly Val Val Thr Asp Gly Val Val Ala Gln Asp Glu Thr Glu Leu Gln
370 375 380
Asn Leu Trp Lys Trp Arg Glu Met Ile Pro Glu Ala Ser Gln Ala Asn
385 390 395 400
Gly Gly Val Tyr Lys Tyr Asp Val Ser Leu Pro Leu Lys Asp Leu Tyr
405 410 415
Ser Leu Val Asp Ala Thr Asn Ala Arg Leu Ser Glu Ala Gly Leu Val
420 425 430
Gly Asp Ser Pro Lys Pro Val Val Gly Ala Ile Gly Tyr Gly His Val
435 440 445
Gly Asp Gly Asn Leu His Leu Asn Val Ala Val Arg Glu Tyr Asn Lys
450 455 460
Glu Ile Glu Lys Val Leu Glu Pro Phe Val Tyr Glu Phe Val Ser Ser
465 470 475 480
Lys His Gly Ser Val Ser Ala Glu His Gly Leu Gly Phe Gln Lys Lys
485 490 495
Asn Tyr Ile Gly Tyr Ser Lys Ser Pro Glu Glu Val Thr Met Ile Lys
500 505 510
Asp Leu Lys Ala His Tyr Asp Pro Asn Ala Ile Leu Asn Pro Tyr Lys
515 520 525
Tyr Val
530
<210> 67
<211> 4721
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和烟曲霉。
<400> 67
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atggtcggat caatagtctt 1560
attacaacca tggaagagag cagtttccca cgcagttaga cctagaactt accaatacag 1620
atacttcaga acaactgcta gaatatcagc taacgacaaa ccacaatcat tcaaaaacca 1680
actatacgaa tcaacacaac aacgtttgag aagagaaaga gcagaacagg aaagattctc 1740
tcagtaccaa actcagtctc ctggtgggag atacgcggct ttaaccttcg cacttgtatt 1800
cttttctaca ggtgcttact acctgggaag cctcaagcca gccagtttac caacatcatc 1860
tacaaccacc ctttttgaaa ttgaacctcc actgcacaat atcagtccag caaatttgca 1920
agccgcttgg gcagatttcg ttgaaattct aggtaaagag aatgtctcaa cagaacatgg 1980
tgatcttgat gttcattctg gtagcgattg gagttcatat actctgaaaa aggacgaaag 2040
acctttctta gttctctacc catctactac agaggaagtt agcagaatta tgaaggtgtg 2100
tcaccagaga gtgatccctg tcacaccata ctcaggaggc acatcactcg aagggcactt 2160
tgctccaaca agaggtggtg tctgtattga ttttagacgt atgaacagaa tcttggagtt 2220
gcataaaaag gacttggatg ttgttgttca accagcagtt ggatgggagg accttaatga 2280
ggaactttct aaggacggct tgtttttccc acctgatcca ggacctggtg ctatgatcgg 2340
tggtatggtt ggtacaggat gctctggaac caacgcatat agatacggaa ccatgagaga 2400
atgggttctt tcactgacag ttgttttggc cgatggcact gttatcaaga caagacaaag 2460
accacgtaaa tcatctgctg gttatgactt aactagattg tttattgggt ctgaaggtac 2520
tttaggtttg gtgactgaag ccaccttgaa gttaacagta aagccaaagt cccagagtgt 2580
tgctgtggct tcttttccat ccattcataa cgccgccgaa tgtgttacta gagtagtcga 2640
ggaaggtata ccagtcgctg gtgtcgagat attggatgac gtgcaaatga agtgcatcaa 2700
cgatagtaga acaacacgta gacaatggaa ggaatcccct acacttttct tcaagttcac 2760
tggcacacca gttggcgtaa aagagcagat tgaattggtt agaaagattg tatcatcttc 2820
agcagggcaa tctttcgagt ttgccagagg tgaagatgag atgaaggaat tgtggtccgc 2880
cagaaaggaa gctttatggt ctgtaatgtc tatgagaaga gggcctgaag atagagtttg 2940
gacaaccgac gtggcggtcc ctatgtctaa gttaccagac atcattgaag caaccaaaca 3000
agatatgaca gagtccggct tactagcagg gatatgtggt catgttggtg atggtaactt 3060
ccacgcaatc attttgttca acgaagatga gaaaaagatt gctgaaggtg ttgtacatag 3120
aatggtgaag agagcggtcg agatggaggg caccgtaact ggagaacatg gcgtgggtct 3180
aatcaaaaga gattacctac aacatgaagt cggtgaaact actgtagata caatgcgtag 3240
aatcaaaatg gcattagatc cactaagatt gctaaattgc gataaggtgg ttagagtcga 3300
gcaaccaact acagttgaat tgaaaaagtg gtgattaatt aaacaggccc cttttccttt 3360
gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc cacatccgct 3420
ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 3480
gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 3540
cgcgtgtacg catgtaacgg gcagacggcc ggccataact tcgtataatg tatgctatac 3600
gaagttatgg caacggttca tcatctcatg gatctgcaca tgaacaaaca ccagagtcaa 3660
acgacgttga aattgaggct actgcgccaa ttgatgacaa tacagacgat gataacaaac 3720
cgaagttatc tgatgtagaa aaggattaga gatgctaaga gatagtgatg atatttcata 3780
aataatgtaa ttctatatat gttaattacc ttttttgcga ggcatattta tggtgaagga 3840
taagttttga ccatcaaaga aggttaatgt ggctgtggtt tcagggtcca taaagctttt 3900
caattcatct tttttttttt tgttcttttt tttgattccg gtttctttga aatttttttg 3960
attcggtaat ctccgagcag aaggaagaac gaaggaagga gcacagactt agattggtat 4020
atatacgcat atgtggtgtt gaagaaacat gaaattgccc agtattctta acccaactgc 4080
acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac 4140
gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc 4200
aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg 4260
aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt 4320
ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct 4380
tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg 4440
tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta 4500
ttgttagcgg tttgaagcag gcggcggaag aagtaacaaa ggaacctaga ggccttttga 4560
tgttagcaga attgtcatgc aagggctccc tagctactgg agaatatact aagggtactg 4620
ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg 4680
gtggaagaga tgaaggttac gattggttga ttatgacacg c 4721
<210> 68
<211> 597
<212> PRT
<213> 烟曲霉(Aspergillus fumigatus)
<400> 68
Met Val Gly Ser Ile Val Leu Leu Gln Pro Trp Lys Arg Ala Val Ser
1 5 10 15
His Ala Val Arg Pro Arg Thr Tyr Gln Tyr Arg Tyr Phe Arg Thr Thr
20 25 30
Ala Arg Ile Ser Ala Asn Asp Lys Pro Gln Ser Phe Lys Asn Gln Leu
35 40 45
Tyr Glu Ser Thr Gln Gln Arg Leu Arg Arg Glu Arg Ala Glu Gln Glu
50 55 60
Arg Phe Ser Gln Tyr Gln Thr Gln Ser Pro Gly Gly Arg Tyr Ala Ala
65 70 75 80
Leu Thr Phe Ala Leu Val Phe Phe Ser Thr Gly Ala Tyr Tyr Leu Gly
85 90 95
Ser Leu Lys Pro Ala Ser Leu Pro Thr Ser Ser Thr Thr Thr Leu Phe
100 105 110
Glu Ile Glu Pro Pro Leu His Asn Ile Ser Pro Ala Asn Leu Gln Ala
115 120 125
Ala Trp Ala Asp Phe Val Glu Ile Leu Gly Lys Glu Asn Val Ser Thr
130 135 140
Glu His Gly Asp Leu Asp Val His Ser Gly Ser Asp Trp Ser Ser Tyr
145 150 155 160
Thr Leu Lys Lys Asp Glu Arg Pro Phe Leu Val Leu Tyr Pro Ser Thr
165 170 175
Thr Glu Glu Val Ser Arg Ile Met Lys Val Cys His Gln Arg Val Ile
180 185 190
Pro Val Thr Pro Tyr Ser Gly Gly Thr Ser Leu Glu Gly His Phe Ala
195 200 205
Pro Thr Arg Gly Gly Val Cys Ile Asp Phe Arg Arg Met Asn Arg Ile
210 215 220
Leu Glu Leu His Lys Lys Asp Leu Asp Val Val Val Gln Pro Ala Val
225 230 235 240
Gly Trp Glu Asp Leu Asn Glu Glu Leu Ser Lys Asp Gly Leu Phe Phe
245 250 255
Pro Pro Asp Pro Gly Pro Gly Ala Met Ile Gly Gly Met Val Gly Thr
260 265 270
Gly Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Arg Glu Trp
275 280 285
Val Leu Ser Leu Thr Val Val Leu Ala Asp Gly Thr Val Ile Lys Thr
290 295 300
Arg Gln Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asp Leu Thr Arg Leu
305 310 315 320
Phe Ile Gly Ser Glu Gly Thr Leu Gly Leu Val Thr Glu Ala Thr Leu
325 330 335
Lys Leu Thr Val Lys Pro Lys Ser Gln Ser Val Ala Val Ala Ser Phe
340 345 350
Pro Ser Ile His Asn Ala Ala Glu Cys Val Thr Arg Val Val Glu Glu
355 360 365
Gly Ile Pro Val Ala Gly Val Glu Ile Leu Asp Asp Val Gln Met Lys
370 375 380
Cys Ile Asn Asp Ser Arg Thr Thr Arg Arg Gln Trp Lys Glu Ser Pro
385 390 395 400
Thr Leu Phe Phe Lys Phe Thr Gly Thr Pro Val Gly Val Lys Glu Gln
405 410 415
Ile Glu Leu Val Arg Lys Ile Val Ser Ser Ser Ala Gly Gln Ser Phe
420 425 430
Glu Phe Ala Arg Gly Glu Asp Glu Met Lys Glu Leu Trp Ser Ala Arg
435 440 445
Lys Glu Ala Leu Trp Ser Val Met Ser Met Arg Arg Gly Pro Glu Asp
450 455 460
Arg Val Trp Thr Thr Asp Val Ala Val Pro Met Ser Lys Leu Pro Asp
465 470 475 480
Ile Ile Glu Ala Thr Lys Gln Asp Met Thr Glu Ser Gly Leu Leu Ala
485 490 495
Gly Ile Cys Gly His Val Gly Asp Gly Asn Phe His Ala Ile Ile Leu
500 505 510
Phe Asn Glu Asp Glu Lys Lys Ile Ala Glu Gly Val Val His Arg Met
515 520 525
Val Lys Arg Ala Val Glu Met Glu Gly Thr Val Thr Gly Glu His Gly
530 535 540
Val Gly Leu Ile Lys Arg Asp Tyr Leu Gln His Glu Val Gly Glu Thr
545 550 555 560
Thr Val Asp Thr Met Arg Arg Ile Lys Met Ala Leu Asp Pro Leu Arg
565 570 575
Leu Leu Asn Cys Asp Lys Val Val Arg Val Glu Gln Pro Thr Thr Val
580 585 590
Glu Leu Lys Lys Trp
595
<210> 69
<211> 4652
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和乳酸克鲁维酵母。
<400> 69
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgttcaggt ttgttggaag 1560
atccggtttt gctttgcgtg gttcgctgca actacgtaag gacgttctgc gttctaggac 1620
cactgccgtt gcaaaaagac attattcgtc ttctaacggt aataacggcg gtggattttc 1680
gtccgccatt ttaagcgttc ttggtggttc tttaattggt ggtgggtttg ttgcatatgc 1740
tctaggctct cagttcgaga aggaaaagtc cgtgagtgat ttgtccattg ctagattgga 1800
agaccttgat tctcccgagt actgtgataa ggaaactttt gctaaagctc ttgttgaatt 1860
gaaagatgtc ttagaaaacg atcctgaaaa cttcactgtg gctaaggacg atttggatgc 1920
tcattcggat acctatttca actctcatca tgctgaagct aatcaaagac cagaaattgt 1980
tttgtatcca cgcaacacag aagatgtttc caaattattg aaaatctgtc acaaatactc 2040
tatcccagtg atcccatttt caggtggtac ctctttggaa gggcatttcc taccaactag 2100
accagggtct tgtgtcgtct tggatatttc aaaatatttg aataagatta ttcaattgaa 2160
taaagaggat ttggatgttg tggtacaagg tggtgttcca tgggaagaat tgaacgaata 2220
tttgaacgat catggtttgt tgttcggttg tgaccctggt ccaggtgctc aaatcgccgg 2280
ttgcattgct aattcttgtt ctggtaccaa cgcttatcgt tatggtacca tgaaggaaaa 2340
cgttgttaat attactatgt gtatggctga tggtactatt gttaagacca agagaagacc 2400
tagaaaatcg tctgctggtt acaatttgaa cgggttaatt attggtagtg aaggtacttt 2460
gggtatcgtt actgaagcta ctattaaatg tcatgttaga tctacttttg aaactgtcgc 2520
tgtcgttccg ttcccaactg tcagtgatgc tgcatcttgt tcctctcatt tgattcaagc 2580
cggtattcaa ttgaatgcta tggaattgtt ggatgataac atgatgaaaa tcatcaacca 2640
aagtggtgct acttcaaagg ataactgggt cgaatctcca actttgttct tcaagattgg 2700
tggtagatct gaacagatta ttcaagaagt cattaaagaa gtggaaaaaa ttgcttctca 2760
acacaacaat accaaatttg aatttgctac tgatgaagat tccaagctag aactatggga 2820
agctagaaaa gttgctcttt ggtcaaccat tgataccggc agaaagacca acccagatgc 2880
caacatttgg acaaccgatg ttgctgttcc aatctcgaaa ttcgcagatg ttattaatgc 2940
taccaaggaa gaaatgaacg catctggttt attaacctct ttggttggtc atgctggtga 3000
tggtaacttc catgcattca tcatctacaa cactgaacaa agaaaaacag ctgaaactat 3060
tgttgaaaat atggtcaaga gagccattga tgctgaaggt acttgtaccg gtgaacacgg 3120
tgtcggtatc ggtaagagag actacttgtt ggaagaagtt ggtgaagaca ctgttgcagt 3180
tatgagaaag ttgaagctcg ctttggatcc taagagaatc ttgaatccag ataagatctt 3240
caagattgat ccaaacgatc atcaacatta attaaacagg ccccttttcc tttgtcgata 3300
tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg 3360
aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt 3420
tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac aaacgcgtgt 3480
acgcatgtaa cgggcagacg gccggccata acttcgtata atgtatgcta tacgaagtta 3540
tggcaacggt tcatcatctc atggatctgc acatgaacaa acaccagagt caaacgacgt 3600
tgaaattgag gctactgcgc caattgatga caatacagac gatgataaca aaccgaagtt 3660
atctgatgta gaaaaggatt agagatgcta agagatagtg atgatatttc ataaataatg 3720
taattctata tatgttaatt accttttttg cgaggcatat ttatggtgaa ggataagttt 3780
tgaccatcaa agaaggttaa tgtggctgtg gtttcagggt ccataaagct tttcaattca 3840
tctttttttt ttttgttctt ttttttgatt ccggtttctt tgaaattttt ttgattcggt 3900
aatctccgag cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg 3960
catatgtggt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac 4020
aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc 4080
tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa 4140
cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt 4200
aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga 4260
gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga 4320
cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag 4380
aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag 4440
cggtttgaag caggcggcgg aagaagtaac aaaggaacct agaggccttt tgatgttagc 4500
agaattgtca tgcaagggct ccctagctac tggagaatat actaagggta ctgttgacat 4560
tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag 4620
agatgaaggt tacgattggt tgattatgac ac 4652
<210> 70
<211> 576
<212> PRT
<213> 乳酸克鲁维酵母(Kluyveromyces lactis)
<400> 70
Met Phe Arg Phe Val Gly Arg Ser Gly Phe Ala Leu Arg Gly Ser Leu
1 5 10 15
Gln Leu Arg Lys Asp Val Leu Arg Ser Arg Thr Thr Ala Val Ala Lys
20 25 30
Arg His Tyr Ser Ser Ser Asn Gly Asn Asn Gly Gly Gly Phe Ser Ser
35 40 45
Ala Ile Leu Ser Val Leu Gly Gly Ser Leu Ile Gly Gly Gly Phe Val
50 55 60
Ala Tyr Ala Leu Gly Ser Gln Phe Glu Lys Glu Lys Ser Val Ser Asp
65 70 75 80
Leu Ser Ile Ala Arg Leu Glu Asp Leu Asp Ser Pro Glu Tyr Cys Asp
85 90 95
Lys Glu Thr Phe Ala Lys Ala Leu Val Glu Leu Lys Asp Val Leu Glu
100 105 110
Asn Asp Pro Glu Asn Phe Thr Val Ala Lys Asp Asp Leu Asp Ala His
115 120 125
Ser Asp Thr Tyr Phe Asn Ser His His Ala Glu Ala Asn Gln Arg Pro
130 135 140
Glu Ile Val Leu Tyr Pro Arg Asn Thr Glu Asp Val Ser Lys Leu Leu
145 150 155 160
Lys Ile Cys His Lys Tyr Ser Ile Pro Val Ile Pro Phe Ser Gly Gly
165 170 175
Thr Ser Leu Glu Gly His Phe Leu Pro Thr Arg Pro Gly Ser Cys Val
180 185 190
Val Leu Asp Ile Ser Lys Tyr Leu Asn Lys Ile Ile Gln Leu Asn Lys
195 200 205
Glu Asp Leu Asp Val Val Val Gln Gly Gly Val Pro Trp Glu Glu Leu
210 215 220
Asn Glu Tyr Leu Asn Asp His Gly Leu Leu Phe Gly Cys Asp Pro Gly
225 230 235 240
Pro Gly Ala Gln Ile Ala Gly Cys Ile Ala Asn Ser Cys Ser Gly Thr
245 250 255
Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu Asn Val Val Asn Ile Thr
260 265 270
Met Cys Met Ala Asp Gly Thr Ile Val Lys Thr Lys Arg Arg Pro Arg
275 280 285
Lys Ser Ser Ala Gly Tyr Asn Leu Asn Gly Leu Ile Ile Gly Ser Glu
290 295 300
Gly Thr Leu Gly Ile Val Thr Glu Ala Thr Ile Lys Cys His Val Arg
305 310 315 320
Ser Thr Phe Glu Thr Val Ala Val Val Pro Phe Pro Thr Val Ser Asp
325 330 335
Ala Ala Ser Cys Ser Ser His Leu Ile Gln Ala Gly Ile Gln Leu Asn
340 345 350
Ala Met Glu Leu Leu Asp Asp Asn Met Met Lys Ile Ile Asn Gln Ser
355 360 365
Gly Ala Thr Ser Lys Asp Asn Trp Val Glu Ser Pro Thr Leu Phe Phe
370 375 380
Lys Ile Gly Gly Arg Ser Glu Gln Ile Ile Gln Glu Val Ile Lys Glu
385 390 395 400
Val Glu Lys Ile Ala Ser Gln His Asn Asn Thr Lys Phe Glu Phe Ala
405 410 415
Thr Asp Glu Asp Ser Lys Leu Glu Leu Trp Glu Ala Arg Lys Val Ala
420 425 430
Leu Trp Ser Thr Ile Asp Thr Gly Arg Lys Thr Asn Pro Asp Ala Asn
435 440 445
Ile Trp Thr Thr Asp Val Ala Val Pro Ile Ser Lys Phe Ala Asp Val
450 455 460
Ile Asn Ala Thr Lys Glu Glu Met Asn Ala Ser Gly Leu Leu Thr Ser
465 470 475 480
Leu Val Gly His Ala Gly Asp Gly Asn Phe His Ala Phe Ile Ile Tyr
485 490 495
Asn Thr Glu Gln Arg Lys Thr Ala Glu Thr Ile Val Glu Asn Met Val
500 505 510
Lys Arg Ala Ile Asp Ala Glu Gly Thr Cys Thr Gly Glu His Gly Val
515 520 525
Gly Ile Gly Lys Arg Asp Tyr Leu Leu Glu Glu Val Gly Glu Asp Thr
530 535 540
Val Ala Val Met Arg Lys Leu Lys Leu Ala Leu Asp Pro Lys Arg Ile
545 550 555 560
Leu Asn Pro Asp Lys Ile Phe Lys Ile Asp Pro Asn Asp His Gln His
565 570 575
<210> 71
<211> 4676
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和多布克鲁维酵母。
<400> 71
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgttcaaat ttgttggtag 1560
aagtggcaca gccttgagat cttcattgca agcacgtaga aatggagcgg tttggaagag 1620
aggatacgca tctggttctg gtagcggtag tggttcaggt agtggctcat catcactcgt 1680
tctaggagtg ttagccggat ctatgatagg aggtggtatt gtggcataca cattaggtaa 1740
ccaattcgaa aaggacaaaa aggtttccgg ctcctctgct gctgccgcag cttctgattt 1800
atccatcttg aagttggagg acttagagag cccagtttat tgtgactccg aaactttttc 1860
aacagcagtt gaagagttaa agaccgtgct gaacaatgac cctgaaaact ttaccacagc 1920
taaagcagat cttgacagcc attctgatac ttacttcaac tctcatcacg ctgaaccaga 1980
tcaaagacca gaaatcgttt tgttccctag aaacactgaa gatgtctctc agttgttgaa 2040
gatctgtcat aagtactcta tccctgtcat tccattttct ggcggtacat cattggaagg 2100
tcatttccta ccaactagac ctggttcctg cgtcgtacta gatattagta aatacttgaa 2160
taagatcata catcttaaca aggaggacct cgacgtcgtt gttcaagggg gggtaccatg 2220
ggaagatttg aacgattacc ttattgacca aggtctgttg ttcggctgcg atccaggccc 2280
aggcgcacag atcgctggtt gcatagctaa ttcatgtagt ggaacaaacg catacagata 2340
cgggactatg aaagagaatg tagttaacat cacaatgtgt atggccgatg gtaccattat 2400
caagaccaaa aagcgtccac gtaaatctgc agccggttac aatcttaatg gtctaattgt 2460
cggctctgaa ggtacattgg gtattgtgac cgaggccaca atcaagtgcc acgtcagatc 2520
tttattcgag acagtagcag ttgttccttt cccaacagtc tctgatgccg ctagttgttc 2580
atcacatctt atacaagcgg gtattcaact aaacgccatg gaactgttag atgataacat 2640
gatgaagatt atcaatcaat ctggggcaac ttccaaaatt gattgggttg agtcaccaac 2700
actattcttt aagatagggg gtagaaccga aaaatctatt caagatgtgg tagatgaagt 2760
tgaaaagatt gcgtcacaac acaacaatta caagttcgaa ttcgcaacag atgccgattc 2820
taagctagaa ttatgggaag ctagaaaagt tgctttgtgg agcactattg atgcagggaa 2880
aaagttagat cctaaagtca acgtgtggac tactgatgtt gcagtaccaa tctcaagatt 2940
tgccgaagtg atcaacgcta caaaacaaga gatgaactct tcaggcttat tgacttcact 3000
tgtcggccat gcgggtgacg gtaactttca cgctttcatc atctataact ccgaacagag 3060
aaagcaggct gagacaatag ttgaaaacat ggttaagaga gcaattgatg ctgaaggaac 3120
atgtactgga gaacatggag tgggtattgg taagagagac tacttagtag aggaattagg 3180
agaggacgca atcgctgtta tgagaaagct taagttggct ctcgatccta agagaatact 3240
gaatccagat aagattttca aaattgatcc aaacgataat cagcactagt taattaaaca 3300
ggcccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct 3360
cctcccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct 3420
atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt 3480
ttttttctgt acaaacgcgt gtacgcatgt aacgggcaga cggccggcca taacttcgta 3540
taatgtatgc tatacgaagt tatggcaacg gttcatcatc tcatggatct gcacatgaac 3600
aaacaccaga gtcaaacgac gttgaaattg aggctactgc gccaattgat gacaatacag 3660
acgatgataa caaaccgaag ttatctgatg tagaaaagga ttagagatgc taagagatag 3720
tgatgatatt tcataaataa tgtaattcta tatatgttaa ttaccttttt tgcgaggcat 3780
atttatggtg aaggataagt tttgaccatc aaagaaggtt aatgtggctg tggtttcagg 3840
gtccataaag cttttcaatt catctttttt ttttttgttc ttttttttga ttccggtttc 3900
tttgaaattt ttttgattcg gtaatctccg agcagaagga agaacgaagg aaggagcaca 3960
gacttagatt ggtatatata cgcatatgtg gtgttgaaga aacatgaaat tgcccagtat 4020
tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 4080
ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 4140
tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 4200
tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 4260
tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 4320
acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 4380
agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 4440
tggtgggccc aggtattgtt agcggtttga agcaggcggc ggaagaagta acaaaggaac 4500
ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctagct actggagaat 4560
atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 4620
ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacgc 4676
<210> 72
<211> 582
<212> PRT
<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)
<400> 72
Met Phe Lys Phe Val Gly Arg Ser Gly Thr Ala Leu Arg Ser Ser Leu
1 5 10 15
Gln Ala Arg Arg Asn Gly Ala Val Trp Lys Arg Gly Tyr Ala Ser Gly
20 25 30
Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Ser Ser Leu Val Leu
35 40 45
Gly Val Leu Ala Gly Ser Met Ile Gly Gly Gly Ile Val Ala Tyr Thr
50 55 60
Leu Gly Asn Gln Phe Glu Lys Asp Lys Lys Val Ser Gly Ser Ser Ala
65 70 75 80
Ala Ala Ala Ala Ser Asp Leu Ser Ile Leu Lys Leu Glu Asp Leu Glu
85 90 95
Ser Pro Val Tyr Cys Asp Ser Glu Thr Phe Ser Thr Ala Val Glu Glu
100 105 110
Leu Lys Thr Val Leu Asn Asn Asp Pro Glu Asn Phe Thr Thr Ala Lys
115 120 125
Ala Asp Leu Asp Ser His Ser Asp Thr Tyr Phe Asn Ser His His Ala
130 135 140
Glu Pro Asp Gln Arg Pro Glu Ile Val Leu Phe Pro Arg Asn Thr Glu
145 150 155 160
Asp Val Ser Gln Leu Leu Lys Ile Cys His Lys Tyr Ser Ile Pro Val
165 170 175
Ile Pro Phe Ser Gly Gly Thr Ser Leu Glu Gly His Phe Leu Pro Thr
180 185 190
Arg Pro Gly Ser Cys Val Val Leu Asp Ile Ser Lys Tyr Leu Asn Lys
195 200 205
Ile Ile His Leu Asn Lys Glu Asp Leu Asp Val Val Val Gln Gly Gly
210 215 220
Val Pro Trp Glu Asp Leu Asn Asp Tyr Leu Ile Asp Gln Gly Leu Leu
225 230 235 240
Phe Gly Cys Asp Pro Gly Pro Gly Ala Gln Ile Ala Gly Cys Ile Ala
245 250 255
Asn Ser Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu
260 265 270
Asn Val Val Asn Ile Thr Met Cys Met Ala Asp Gly Thr Ile Ile Lys
275 280 285
Thr Lys Lys Arg Pro Arg Lys Ser Ala Ala Gly Tyr Asn Leu Asn Gly
290 295 300
Leu Ile Val Gly Ser Glu Gly Thr Leu Gly Ile Val Thr Glu Ala Thr
305 310 315 320
Ile Lys Cys His Val Arg Ser Leu Phe Glu Thr Val Ala Val Val Pro
325 330 335
Phe Pro Thr Val Ser Asp Ala Ala Ser Cys Ser Ser His Leu Ile Gln
340 345 350
Ala Gly Ile Gln Leu Asn Ala Met Glu Leu Leu Asp Asp Asn Met Met
355 360 365
Lys Ile Ile Asn Gln Ser Gly Ala Thr Ser Lys Ile Asp Trp Val Glu
370 375 380
Ser Pro Thr Leu Phe Phe Lys Ile Gly Gly Arg Thr Glu Lys Ser Ile
385 390 395 400
Gln Asp Val Val Asp Glu Val Glu Lys Ile Ala Ser Gln His Asn Asn
405 410 415
Tyr Lys Phe Glu Phe Ala Thr Asp Ala Asp Ser Lys Leu Glu Leu Trp
420 425 430
Glu Ala Arg Lys Val Ala Leu Trp Ser Thr Ile Asp Ala Gly Lys Lys
435 440 445
Leu Asp Pro Lys Val Asn Val Trp Thr Thr Asp Val Ala Val Pro Ile
450 455 460
Ser Arg Phe Ala Glu Val Ile Asn Ala Thr Lys Gln Glu Met Asn Ser
465 470 475 480
Ser Gly Leu Leu Thr Ser Leu Val Gly His Ala Gly Asp Gly Asn Phe
485 490 495
His Ala Phe Ile Ile Tyr Asn Ser Glu Gln Arg Lys Gln Ala Glu Thr
500 505 510
Ile Val Glu Asn Met Val Lys Arg Ala Ile Asp Ala Glu Gly Thr Cys
515 520 525
Thr Gly Glu His Gly Val Gly Ile Gly Lys Arg Asp Tyr Leu Val Glu
530 535 540
Glu Leu Gly Glu Asp Ala Ile Ala Val Met Arg Lys Leu Lys Leu Ala
545 550 555 560
Leu Asp Pro Lys Arg Ile Leu Asn Pro Asp Lys Ile Phe Lys Ile Asp
565 570 575
Pro Asn Asp Asn Gln His
580
<210> 73
<211> 4704
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和马克思克鲁维酵母。
<400> 73
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agatctcccg agtttatcat tatcaatact gccatttcaa agaatacgta 900
aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattggc cttttaattc 960
tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcata 1020
ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgctttttt 1080
taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac 1140
catcagttca taggtccatt ctcttagcgc aactacacag aacaggggca caaacaggca 1200
aaaaacgggc acaacctcaa tggagtgatg caacctgctt ggagtaaatg atgacacaag 1260
gcaattgacc tacgcatgta tctatctcat tttcttacac cttctattac cttctgctct 1320
ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct 1380
atttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc 1440
ttaaacttct taaattctac ttttatagtt agtctttttt ttagtttaaa acaccaagaa 1500
cttagtttcg aataaacaca cataaacaaa caaatctaga atgttaccaa gatttgttgt 1560
gagatcaggc gctgcaggga gaaatttggg tttcagcttc acacgtaaat gtgaccacac 1620
ttttctgagc aagaccgtca gaaacgattt atctcataga ccatacagta caggtactaa 1680
tggaaacgga tctgcaaatg ctggtaaaag tcaatctggt ctattgttcg gtgtattcgg 1740
aggaactttg attgggggag gcttggttgc atacttcttg ggttctaagt tcgatcaaca 1800
acaatcttcc tcacaacaag tttctgacct ctcaatagcg agacttgaag atttagactc 1860
ccctaagtac tgcgacaaaa agacctttgc aactgctgtc gaggaactga agcaagtctt 1920
agataacaac cctgagaact tctctgacgc caagtcagat ttggattccc actcagatac 1980
atactttaac tctcatcacg caactccaga acaaagacca gaaatcgtac tattccctag 2040
aaacacagag gacgtttcta agctactcaa gatttgccac aaatactcaa tcccagtaat 2100
cccattctcc ggtggtacct cacttgaagg tcatttcatg ccaaccagac caggttcatg 2160
tgtagtgttg gacatttcta aatacatgaa tcagattatt caactaaaca aggaagattt 2220
ggatgttgtg gtacaaggtg gcgttccttg ggaggatttg aatgattacc ttaatgatca 2280
tgggttgtta tttggatgtg acccaggtcc aggggctcaa atagccggtt gcatcgctaa 2340
ttcttgttct gggaccaacg catatcgtta cggcactatg aaggaaaacg ttgttaacat 2400
aacaatgtgc ctcgcagatg gtactattat caagactaag cgtagaccta gaaagtcaag 2460
tgccggttac aacttaaacg gtttgataat cgggtccgaa ggtactttag gaattgtgac 2520
agaagcgaca atcaagtgcc atgtcagatc aaatttcgaa actgtcgcag tcgttccatt 2580
cccttcagtt gctgatgctg cttcttgttc ttctcatttg attcaggctg gcattcagtt 2640
gaacgccatg gaattgctcg atgataacat gatgaagatc atcaataagt ccggagcaac 2700
tagtagaaca aattgggtag aatcaccaac ccttttcttc aagataggtg gtagaagcga 2760
aaaagctatt aaggaagttg ttaaagaggt tgagaaaatt gcttcacagc acaataactc 2820
taactttgaa tttgcatcag atgaagagac aaaactagaa ctttgggagg ccagaaaggt 2880
tgccctgtgg tccacaattg atgcaggcaa aaagctagat cctaacgtga acgtttggac 2940
aacagatgtt gcagtcccaa taagtaaatt cgcacaagtc atcaatgata caaaggagga 3000
aatgaacgcg tctggcctgt taacctcatt ggtcggtcat gctggtgatg gtaactttca 3060
tgcgtttatc atctataacg ctgaacagag aaagactgct gaaacaatcg tagagaacat 3120
ggttaaaaga gcaattgatg ccgaaggcac atgtacagga gaacatggag tgggtattgg 3180
caaaagagaa ttccttgttg aggaattagg tgaggataca attgccgtta tgagaaagct 3240
taagttagcc ttagacccaa agagaatctt gaaccctgat aaagtgttta agattgaccc 3300
aaatgaccat caacactagt taattaaaca ggcccctttt cctttgtcga tatcatgtaa 3360
ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac cgaaaaggaa 3420
ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta 3480
agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt 3540
aacgggcaga cggccggcca taacttcgta taatgtatgc tatacgaagt tatggcaacg 3600
gttcatcatc tcatggatct gcacatgaac aaacaccaga gtcaaacgac gttgaaattg 3660
aggctactgc gccaattgat gacaatacag acgatgataa caaaccgaag ttatctgatg 3720
tagaaaagga ttagagatgc taagagatag tgatgatatt tcataaataa tgtaattcta 3780
tatatgttaa ttaccttttt tgcgaggcat atttatggtg aaggataagt tttgaccatc 3840
aaagaaggtt aatgtggctg tggtttcagg gtccataaag cttttcaatt catctttttt 3900
ttttttgttc ttttttttga ttccggtttc tttgaaattt ttttgattcg gtaatctccg 3960
agcagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgtg 4020
gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct 4080
gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc 4140
ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg 4200
cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca 4260
aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag 4320
ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat 4380
ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag 4440
aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga 4500
agcaggcggc ggaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt 4560
catgcaaggg ctccctagct actggagaat atactaaggg tactgttgac attgcgaaga 4620
gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag 4680
gttacgattg gttgattatg acac 4704
<210> 74
<211> 592
<212> PRT
<213> 马克思克鲁维酵母(Kluyveromyces marxianus)
<400> 74
Met Leu Pro Arg Phe Val Val Arg Ser Gly Ala Ala Gly Arg Asn Leu
1 5 10 15
Gly Phe Ser Phe Thr Arg Lys Cys Asp His Thr Phe Leu Ser Lys Thr
20 25 30
Val Arg Asn Asp Leu Ser His Arg Pro Tyr Ser Thr Gly Thr Asn Gly
35 40 45
Asn Gly Ser Ala Asn Ala Gly Lys Ser Gln Ser Gly Leu Leu Phe Gly
50 55 60
Val Phe Gly Gly Thr Leu Ile Gly Gly Gly Leu Val Ala Tyr Phe Leu
65 70 75 80
Gly Ser Lys Phe Asp Gln Gln Gln Ser Ser Ser Gln Gln Val Ser Asp
85 90 95
Leu Ser Ile Ala Arg Leu Glu Asp Leu Asp Ser Pro Lys Tyr Cys Asp
100 105 110
Lys Lys Thr Phe Ala Thr Ala Val Glu Glu Leu Lys Gln Val Leu Asp
115 120 125
Asn Asn Pro Glu Asn Phe Ser Asp Ala Lys Ser Asp Leu Asp Ser His
130 135 140
Ser Asp Thr Tyr Phe Asn Ser His His Ala Thr Pro Glu Gln Arg Pro
145 150 155 160
Glu Ile Val Leu Phe Pro Arg Asn Thr Glu Asp Val Ser Lys Leu Leu
165 170 175
Lys Ile Cys His Lys Tyr Ser Ile Pro Val Ile Pro Phe Ser Gly Gly
180 185 190
Thr Ser Leu Glu Gly His Phe Met Pro Thr Arg Pro Gly Ser Cys Val
195 200 205
Val Leu Asp Ile Ser Lys Tyr Met Asn Gln Ile Ile Gln Leu Asn Lys
210 215 220
Glu Asp Leu Asp Val Val Val Gln Gly Gly Val Pro Trp Glu Asp Leu
225 230 235 240
Asn Asp Tyr Leu Asn Asp His Gly Leu Leu Phe Gly Cys Asp Pro Gly
245 250 255
Pro Gly Ala Gln Ile Ala Gly Cys Ile Ala Asn Ser Cys Ser Gly Thr
260 265 270
Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu Asn Val Val Asn Ile Thr
275 280 285
Met Cys Leu Ala Asp Gly Thr Ile Ile Lys Thr Lys Arg Arg Pro Arg
290 295 300
Lys Ser Ser Ala Gly Tyr Asn Leu Asn Gly Leu Ile Ile Gly Ser Glu
305 310 315 320
Gly Thr Leu Gly Ile Val Thr Glu Ala Thr Ile Lys Cys His Val Arg
325 330 335
Ser Asn Phe Glu Thr Val Ala Val Val Pro Phe Pro Ser Val Ala Asp
340 345 350
Ala Ala Ser Cys Ser Ser His Leu Ile Gln Ala Gly Ile Gln Leu Asn
355 360 365
Ala Met Glu Leu Leu Asp Asp Asn Met Met Lys Ile Ile Asn Lys Ser
370 375 380
Gly Ala Thr Ser Arg Thr Asn Trp Val Glu Ser Pro Thr Leu Phe Phe
385 390 395 400
Lys Ile Gly Gly Arg Ser Glu Lys Ala Ile Lys Glu Val Val Lys Glu
405 410 415
Val Glu Lys Ile Ala Ser Gln His Asn Asn Ser Asn Phe Glu Phe Ala
420 425 430
Ser Asp Glu Glu Thr Lys Leu Glu Leu Trp Glu Ala Arg Lys Val Ala
435 440 445
Leu Trp Ser Thr Ile Asp Ala Gly Lys Lys Leu Asp Pro Asn Val Asn
450 455 460
Val Trp Thr Thr Asp Val Ala Val Pro Ile Ser Lys Phe Ala Gln Val
465 470 475 480
Ile Asn Asp Thr Lys Glu Glu Met Asn Ala Ser Gly Leu Leu Thr Ser
485 490 495
Leu Val Gly His Ala Gly Asp Gly Asn Phe His Ala Phe Ile Ile Tyr
500 505 510
Asn Ala Glu Gln Arg Lys Thr Ala Glu Thr Ile Val Glu Asn Met Val
515 520 525
Lys Arg Ala Ile Asp Ala Glu Gly Thr Cys Thr Gly Glu His Gly Val
530 535 540
Gly Ile Gly Lys Arg Glu Phe Leu Val Glu Glu Leu Gly Glu Asp Thr
545 550 555 560
Ile Ala Val Met Arg Lys Leu Lys Leu Ala Leu Asp Pro Lys Arg Ile
565 570 575
Leu Asn Pro Asp Lys Val Phe Lys Ile Asp Pro Asn Asp His Gln His
580 585 590
<210> 75
<211> 4953
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和多布克鲁维酵母。
<400> 75
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgcata caatttctcc agaatctgac 1740
tccctgaaat catctaagag taacactaac tcacatgaat acatgagtgc aacaaccgat 1800
tacaataacg ggggtgcgtc tgcagcccca ctttctgcta agagaaaaga gacaggaaac 1860
agctacgaac tccaagacca tgctgcgtcc gccagcgcca gtgcatctgc ttcagcctca 1920
gcatcagctt ctgcaagcgc actagctttt gattacgatc cagaagatga cgatgtttca 1980
atcatgatgc aagatgaaga ggttcctgat ttgacctgga agcatgtatc taactatgca 2040
atcactagat ttacttcatt agcccatatt catccagtct caatgcaaaa catcaatcca 2100
ttcccagagt tgagaaagat gacattacac aactggaatt acttctttat gggctacggt 2160
gcgtggcttt gcgctagttg ggctttcttt gctgtatcag tctcaacagc cccattagca 2220
gaactatacg acaaacagac taaagatatt tcttggggtt tgtccttagt cttgttcgtt 2280
agatctgccg gtgctatcat tttcggtatc tggactgaca actattccag aaagtggcca 2340
tacattacat gtcttggatt gtttttgatt tgtcagctgt gcacaccatt tgcgacaaat 2400
tacactcaat tcttaggggt gagatggatc tctggtgtgg ctatgggagg aatttacgca 2460
tgtgcaagtg caacagctat cgaggatgct ccagttaagg caagatcttt cttatccggt 2520
ctattcttta ccgcatacgc aatggggttc attttcgcca ctgttttcta cagagcattc 2580
ttgaacatta atgggaagga ttattggaag atacagtttt ggttctctat atggttgcct 2640
gccgctctta tcttgtggag actagtatgg ccagaaacaa gatactttac aaaggttcta 2700
aaggctagac aactgatgct tgatgatgct attgcggcaa atggcgggca acctctacct 2760
aaaaagacat tccaggaaaa ggttaccaac gtcaaaaaga tcatatcaac ctactggctg 2820
ttattcacct acttgatact cttgcttgtc ggtcctaatt acctgactca cgcctctatg 2880
gacttgttcc caacaatgct tcgtgctcaa ttgaagtttt ccgaagatgc tgtaactgtt 2940
gcaatcgtgg tagtttgcat tggtgcaatt tgtggcggta tgtttttcgg tcaactaatg 3000
gaaataacag gtcgtagact aggattgttg atagccttgg tcatggcagg ctgttttact 3060
tatccagcat tcatgttgaa cactggtcct gccgttctcg gtggtggttt cttcctgtgg 3120
ttctctgtaa ttggcgtctg gggcgttatc cctatccact tgtccgaatt atcacctcca 3180
gaagcaagag ctttagtatc tggattagct taccaacttg gaaatctcgc gtcagctgcc 3240
tctgtcgtta tcgaatctga cttagctgat ctttacccaa tcgcgtggaa cgcagctggt 3300
gaggtgacaa acaaaaacta ctctaaggtt atggctattt tgacaggctc agtggtgatt 3360
ttcacctttg ttttagtctt agttggacat gaaaagttcc acagagattt aagttcccct 3420
cacttgaaag agtacacaga tagagttgat agagccgaag aggctgcagg catgggtagc 3480
tctgccgcat ctgtttcatc aaaaccagaa caaaacttcg ttgagaaggt gtgattaatt 3540
aatttaccag cttactatcc ttcttgaaaa tatgcactct atatctttta gttcttaatt 3600
gcaacacata gatttgctgt ataacgaatt ttatgctatt tttttaattt ggagttcggt 3660
gatgaaagtg tcacagcgaa tttcctcaca tgtagggacc gaattgttta caagttctct 3720
gtaccaccat ggagacatca aagattgaaa atctatggaa agatatggac ggtagcaaca 3780
agaatatagc acgagccgcg gagttcattt cgttactttt gatatcgctc acaactattg 3840
cgaagcgctt cagtgaaaaa atcataagga aaagttgtaa atattattgg tagtattcgt 3900
ttggtaaagt agagggggta atttttcccc tttattttgt tcatacattc ttaaattgct 3960
ttgcctctcc ttttggaaag ctatacttcg gagcactgtt gagcgaaggc tcaggccggc 4020
atatgacgtt ttattacctt tgatcacatt tccacgccat ttcgcattct caccctcata 4080
agtcatacac cgaaaagaaa gtttaaggga tcaatgagct tactataatc tcagtatatt 4140
tatttttatc gatgattcac cacaacaatc ttgctcccga aaagaaagca gacggagtag 4200
aagcatttga aactccttca gaccttcaag tatatatata tatatatata tatgtatatg 4260
tgtacatttt cacgctaata ctaatgtata attagaagat aatttttact catttttcgt 4320
tatcttcacg tcacccgaac ctagaaccaa atgtcatttt cacgatatgt aaatagtgaa 4380
ataggcaaaa acgccaaaaa gtagtaagcg caacatacac taaaccatta aagaatatct 4440
cgaccagaat ctaacagata tacatgttcc gataatgtct gagttaggtg agtattctaa 4500
attagaaaac aaagagctta gaacggagtt tgaattgaca aattttcctt ttccaggcac 4560
aactgataac gactccgatg acggaagcca agggcagaac tctttgaata tcattactcc 4620
tgacatggat gatactctgg ttaatgatgt acttcgagaa aacgataaaa agtctagtat 4680
gagaatggct tttatgaatc tagcaaactc tattcttggt gccggaataa ttactcagcc 4740
gttcgcgatc aaaaatgctg gtatattagg cgggctatta tcatacgtag ccctcggatt 4800
tatagttgat tggacgttaa gacttattgt cattaacttg actcttgctg gcaagagaac 4860
ataccagggt acggtcgaac atgtaatggg taaaaaaggg aaattgctga ttctatttac 4920
aaacgggtta tttgcatttg gtggatgtat tgg 4953
<210> 76
<211> 606
<212> PRT
<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)
<400> 76
Met His Thr Ile Ser Pro Glu Ser Asp Ser Leu Lys Ser Ser Lys Ser
1 5 10 15
Asn Thr Asn Ser His Glu Tyr Met Ser Ala Thr Thr Asp Tyr Asn Asn
20 25 30
Gly Gly Ala Ser Ala Ala Pro Leu Ser Ala Lys Arg Lys Glu Thr Gly
35 40 45
Asn Ser Tyr Glu Leu Gln Asp His Ala Ala Ser Ala Ser Ala Ser Ala
50 55 60
Ser Ala Ser Ala Ser Ala Ser Ala Ser Ala Ser Ala Leu Ala Phe Asp
65 70 75 80
Tyr Asp Pro Glu Asp Asp Asp Val Ser Ile Met Met Gln Asp Glu Glu
85 90 95
Val Pro Asp Leu Thr Trp Lys His Val Ser Asn Tyr Ala Ile Thr Arg
100 105 110
Phe Thr Ser Leu Ala His Ile His Pro Val Ser Met Gln Asn Ile Asn
115 120 125
Pro Phe Pro Glu Leu Arg Lys Met Thr Leu His Asn Trp Asn Tyr Phe
130 135 140
Phe Met Gly Tyr Gly Ala Trp Leu Cys Ala Ser Trp Ala Phe Phe Ala
145 150 155 160
Val Ser Val Ser Thr Ala Pro Leu Ala Glu Leu Tyr Asp Lys Gln Thr
165 170 175
Lys Asp Ile Ser Trp Gly Leu Ser Leu Val Leu Phe Val Arg Ser Ala
180 185 190
Gly Ala Ile Ile Phe Gly Ile Trp Thr Asp Asn Tyr Ser Arg Lys Trp
195 200 205
Pro Tyr Ile Thr Cys Leu Gly Leu Phe Leu Ile Cys Gln Leu Cys Thr
210 215 220
Pro Phe Ala Thr Asn Tyr Thr Gln Phe Leu Gly Val Arg Trp Ile Ser
225 230 235 240
Gly Val Ala Met Gly Gly Ile Tyr Ala Cys Ala Ser Ala Thr Ala Ile
245 250 255
Glu Asp Ala Pro Val Lys Ala Arg Ser Phe Leu Ser Gly Leu Phe Phe
260 265 270
Thr Ala Tyr Ala Met Gly Phe Ile Phe Ala Thr Val Phe Tyr Arg Ala
275 280 285
Phe Leu Asn Ile Asn Gly Lys Asp Tyr Trp Lys Ile Gln Phe Trp Phe
290 295 300
Ser Ile Trp Leu Pro Ala Ala Leu Ile Leu Trp Arg Leu Val Trp Pro
305 310 315 320
Glu Thr Arg Tyr Phe Thr Lys Val Leu Lys Ala Arg Gln Leu Met Leu
325 330 335
Asp Asp Ala Ile Ala Ala Asn Gly Gly Gln Pro Leu Pro Lys Lys Thr
340 345 350
Phe Gln Glu Lys Val Thr Asn Val Lys Lys Ile Ile Ser Thr Tyr Trp
355 360 365
Leu Leu Phe Thr Tyr Leu Ile Leu Leu Leu Val Gly Pro Asn Tyr Leu
370 375 380
Thr His Ala Ser Met Asp Leu Phe Pro Thr Met Leu Arg Ala Gln Leu
385 390 395 400
Lys Phe Ser Glu Asp Ala Val Thr Val Ala Ile Val Val Val Cys Ile
405 410 415
Gly Ala Ile Cys Gly Gly Met Phe Phe Gly Gln Leu Met Glu Ile Thr
420 425 430
Gly Arg Arg Leu Gly Leu Leu Ile Ala Leu Val Met Ala Gly Cys Phe
435 440 445
Thr Tyr Pro Ala Phe Met Leu Asn Thr Gly Pro Ala Val Leu Gly Gly
450 455 460
Gly Phe Phe Leu Trp Phe Ser Val Ile Gly Val Trp Gly Val Ile Pro
465 470 475 480
Ile His Leu Ser Glu Leu Ser Pro Pro Glu Ala Arg Ala Leu Val Ser
485 490 495
Gly Leu Ala Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ser Val Val
500 505 510
Ile Glu Ser Asp Leu Ala Asp Leu Tyr Pro Ile Ala Trp Asn Ala Ala
515 520 525
Gly Glu Val Thr Asn Lys Asn Tyr Ser Lys Val Met Ala Ile Leu Thr
530 535 540
Gly Ser Val Val Ile Phe Thr Phe Val Leu Val Leu Val Gly His Glu
545 550 555 560
Lys Phe His Arg Asp Leu Ser Ser Pro His Leu Lys Glu Tyr Thr Asp
565 570 575
Arg Val Asp Arg Ala Glu Glu Ala Ala Gly Met Gly Ser Ser Ala Ala
580 585 590
Ser Val Ser Ser Lys Pro Glu Gln Asn Phe Val Glu Lys Val
595 600 605
<210> 77
<211> 4947
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和马克思克鲁维酵母。
<400> 77
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgtccg aaggtaatga agagaaaggc 1740
caaggtcagg ggcagggtca agatcacgga cacttcccag aaggcagaac tttccttcac 1800
caagttgaga gtaagagatc tgttcataca catcatagtg tgcctatgaa ctcatcagat 1860
tcacaatcaa atgcgtcaga tcctgaggaa ttggcgccat ttccttctgt tgttgatgaa 1920
tcagaattag acgaatccga actagaggag atacaaaacg aagatgtctc tatcatttac 1980
aaagacgacg aagagaaacc agatttgagc tgggcatcaa tctgcagata cgctggtact 2040
cgtattactt ctttattcca aatccacaag gtgtctatgg ataacattaa tccattccct 2100
gaacttagaa agatgacatt gtccaattgg aactacttct tcatgggcta tttggcttgg 2160
ttgtgtgccg cctgggcctt cttcgctgtt tctgtatcta cagcaccatt agcaaagctc 2220
tatgacaaac caacaaagga catctcttgg ggtttatctc tagttctgtt cgtcagatct 2280
gctggggcta ttatctttgg actctggact gataactact ccagaaagtg gccatacatt 2340
acttgtctgg gcctattctt gatttgtcag ttgtgcacac catgggctaa aacctacaca 2400
caattcttag gcgtaagatg gatatcaggt atcgcaatgg gtggtatata cggttgtgct 2460
tctgccactg ccatagaaga tgcaccagtg aaggccagaa gtatcctctc aggtttgttc 2520
ttttcagcat acgcaatggg attcattttt gccatcatct tctatagagc attccttcat 2580
gttaatggag aaaactactg gaaggtccag ttttggtttt caatattctt gccagccctc 2640
ttgatgctat ggagattggt gtggccagag acaaagtact tcacaaaagt cttaaaggca 2700
agagagctaa tgaagcagga tgctattgca tctaacggtg ggaacccaat tcctaaaaca 2760
accttcaagg aaaagatggc aaacacaaag agaattatct gcaaatactg gcttttgttc 2820
ggatacttgg ttttgctgtt agtaggacca aactacttaa cccatggaag tcaagactta 2880
ttccctacta tgattcgttc tcaattgaat ttcagtgaag atgctgtcac agttgctatt 2940
gtagttgtta acttaggtgg catttgtggt gggcttttct ttggtcaact aatggaagta 3000
acaggtcgta gacttggact gttaattgcg ttgactatgg ctggttgctt tacctaccct 3060
gctttcatgt tgaaaacatc ttccgccgtc ttaggtgcag gctttatgct ctttttctca 3120
gtgtttggtg tgtggggcgt tatccctatc catctaagtg agctgtcacc accagaagcg 3180
agagcattgg tagctggttt agcttaccaa ctaggtaatc ttgcgtccgc cgcatctgtc 3240
gttatagaaa acgatttagc tgacatttac ccacttgcaa gagatgctgc tggtaaagtg 3300
atcaaaaagg actacgccaa ggtcatggca atcttgactg gttctgttgt catctttacc 3360
tttgttatgg ttctgattgg gcatgaaaag tttcacagag atttgtcaag cgcagtgcta 3420
aaggcatata tggaaaaggt tgatagaaac gaggaagatg ctagattggg cgtatcttct 3480
ctttctatta gctcaaagtc caaggaggaa caagttgaaa acgcatagtt aattaattta 3540
ccagcttact atccttcttg aaaatatgca ctctatatct tttagttctt aattgcaaca 3600
catagatttg ctgtataacg aattttatgc tattttttta atttggagtt cggtgatgaa 3660
agtgtcacag cgaatttcct cacatgtagg gaccgaattg tttacaagtt ctctgtacca 3720
ccatggagac atcaaagatt gaaaatctat ggaaagatat ggacggtagc aacaagaata 3780
tagcacgagc cgcggagttc atttcgttac ttttgatatc gctcacaact attgcgaagc 3840
gcttcagtga aaaaatcata aggaaaagtt gtaaatatta ttggtagtat tcgtttggta 3900
aagtagaggg ggtaattttt cccctttatt ttgttcatac attcttaaat tgctttgcct 3960
ctccttttgg aaagctatac ttcggagcac tgttgagcga aggctcaggc cggcatatga 4020
cgttttatta cctttgatca catttccacg ccatttcgca ttctcaccct cataagtcat 4080
acaccgaaaa gaaagtttaa gggatcaatg agcttactat aatctcagta tatttatttt 4140
tatcgatgat tcaccacaac aatcttgctc ccgaaaagaa agcagacgga gtagaagcat 4200
ttgaaactcc ttcagacctt caagtatata tatatatata tatatatgta tatgtgtaca 4260
ttttcacgct aatactaatg tataattaga agataatttt tactcatttt tcgttatctt 4320
cacgtcaccc gaacctagaa ccaaatgtca ttttcacgat atgtaaatag tgaaataggc 4380
aaaaacgcca aaaagtagta agcgcaacat acactaaacc attaaagaat atctcgacca 4440
gaatctaaca gatatacatg ttccgataat gtctgagtta ggtgagtatt ctaaattaga 4500
aaacaaagag cttagaacgg agtttgaatt gacaaatttt ccttttccag gcacaactga 4560
taacgactcc gatgacggaa gccaagggca gaactctttg aatatcatta ctcctgacat 4620
ggatgatact ctggttaatg atgtacttcg agaaaacgat aaaaagtcta gtatgagaat 4680
ggcttttatg aatctagcaa actctattct tggtgccgga ataattactc agccgttcgc 4740
gatcaaaaat gctggtatat taggcgggct attatcatac gtagccctcg gatttatagt 4800
tgattggacg ttaagactta ttgtcattaa cttgactctt gctggcaaga gaacatacca 4860
gggtacggtc gaacatgtaa tgggtaaaaa agggaaattg ctgattctat ttacaaacgg 4920
gttatttgca tttggtggat gtattgg 4947
<210> 78
<211> 604
<212> PRT
<213> 马克思克鲁维酵母(Kluyveromyces marxianus)
<400> 78
Met Ser Glu Gly Asn Glu Glu Lys Gly Gln Gly Gln Gly Gln Gly Gln
1 5 10 15
Asp His Gly His Phe Pro Glu Gly Arg Thr Phe Leu His Gln Val Glu
20 25 30
Ser Lys Arg Ser Val His Thr His His Ser Val Pro Met Asn Ser Ser
35 40 45
Asp Ser Gln Ser Asn Ala Ser Asp Pro Glu Glu Leu Ala Pro Phe Pro
50 55 60
Ser Val Val Asp Glu Ser Glu Leu Asp Glu Ser Glu Leu Glu Glu Ile
65 70 75 80
Gln Asn Glu Asp Val Ser Ile Ile Tyr Lys Asp Asp Glu Glu Lys Pro
85 90 95
Asp Leu Ser Trp Ala Ser Ile Cys Arg Tyr Ala Gly Thr Arg Ile Thr
100 105 110
Ser Leu Phe Gln Ile His Lys Val Ser Met Asp Asn Ile Asn Pro Phe
115 120 125
Pro Glu Leu Arg Lys Met Thr Leu Ser Asn Trp Asn Tyr Phe Phe Met
130 135 140
Gly Tyr Leu Ala Trp Leu Cys Ala Ala Trp Ala Phe Phe Ala Val Ser
145 150 155 160
Val Ser Thr Ala Pro Leu Ala Lys Leu Tyr Asp Lys Pro Thr Lys Asp
165 170 175
Ile Ser Trp Gly Leu Ser Leu Val Leu Phe Val Arg Ser Ala Gly Ala
180 185 190
Ile Ile Phe Gly Leu Trp Thr Asp Asn Tyr Ser Arg Lys Trp Pro Tyr
195 200 205
Ile Thr Cys Leu Gly Leu Phe Leu Ile Cys Gln Leu Cys Thr Pro Trp
210 215 220
Ala Lys Thr Tyr Thr Gln Phe Leu Gly Val Arg Trp Ile Ser Gly Ile
225 230 235 240
Ala Met Gly Gly Ile Tyr Gly Cys Ala Ser Ala Thr Ala Ile Glu Asp
245 250 255
Ala Pro Val Lys Ala Arg Ser Ile Leu Ser Gly Leu Phe Phe Ser Ala
260 265 270
Tyr Ala Met Gly Phe Ile Phe Ala Ile Ile Phe Tyr Arg Ala Phe Leu
275 280 285
His Val Asn Gly Glu Asn Tyr Trp Lys Val Gln Phe Trp Phe Ser Ile
290 295 300
Phe Leu Pro Ala Leu Leu Met Leu Trp Arg Leu Val Trp Pro Glu Thr
305 310 315 320
Lys Tyr Phe Thr Lys Val Leu Lys Ala Arg Glu Leu Met Lys Gln Asp
325 330 335
Ala Ile Ala Ser Asn Gly Gly Asn Pro Ile Pro Lys Thr Thr Phe Lys
340 345 350
Glu Lys Met Ala Asn Thr Lys Arg Ile Ile Cys Lys Tyr Trp Leu Leu
355 360 365
Phe Gly Tyr Leu Val Leu Leu Leu Val Gly Pro Asn Tyr Leu Thr His
370 375 380
Gly Ser Gln Asp Leu Phe Pro Thr Met Ile Arg Ser Gln Leu Asn Phe
385 390 395 400
Ser Glu Asp Ala Val Thr Val Ala Ile Val Val Val Asn Leu Gly Gly
405 410 415
Ile Cys Gly Gly Leu Phe Phe Gly Gln Leu Met Glu Val Thr Gly Arg
420 425 430
Arg Leu Gly Leu Leu Ile Ala Leu Thr Met Ala Gly Cys Phe Thr Tyr
435 440 445
Pro Ala Phe Met Leu Lys Thr Ser Ser Ala Val Leu Gly Ala Gly Phe
450 455 460
Met Leu Phe Phe Ser Val Phe Gly Val Trp Gly Val Ile Pro Ile His
465 470 475 480
Leu Ser Glu Leu Ser Pro Pro Glu Ala Arg Ala Leu Val Ala Gly Leu
485 490 495
Ala Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ser Val Val Ile Glu
500 505 510
Asn Asp Leu Ala Asp Ile Tyr Pro Leu Ala Arg Asp Ala Ala Gly Lys
515 520 525
Val Ile Lys Lys Asp Tyr Ala Lys Val Met Ala Ile Leu Thr Gly Ser
530 535 540
Val Val Ile Phe Thr Phe Val Met Val Leu Ile Gly His Glu Lys Phe
545 550 555 560
His Arg Asp Leu Ser Ser Ala Val Leu Lys Ala Tyr Met Glu Lys Val
565 570 575
Asp Arg Asn Glu Glu Asp Ala Arg Leu Gly Val Ser Ser Leu Ser Ile
580 585 590
Ser Ser Lys Ser Lys Glu Glu Gln Val Glu Asn Ala
595 600
<210> 79
<211> 4707
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和解脂耶氏酵母。
<400> 79
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatggaag cgccaaatct ttcaccagct 1740
tcaattagaa gatacttcgc aacaagattg cctaccctta ttccacaaaa actaacacca 1800
gaagagaaaa agcttctcaa cccatttcca gcactagctt tgattaacaa aaagacctgg 1860
ctattcattg cctgtgcatt ctgtggttgg acttgggatg cctttgactt tttcagcgtc 1920
ggtctagttg ctccagaaat agcaaagtct ctaaatcgtt cagttaccga tattacttgg 1980
ggtattaccc tggtcttgat gttgcgttct attggagccg tggtatttgg tatcgcatcc 2040
gatagatatg ggagaaagtg gcctttcatc gttaacttac tatgctttat agctctggag 2100
ttgggttctg gcttcgtgca aacatacaag caattcttgg gcgttagagc aatttacggt 2160
atcgctatgg ggggactcta cggtaatgca gcagccactg cgttagagga ctgccctcca 2220
caagcaagag ggataatcag cggtttcctt caatccggat acgcattagg ttacctgtta 2280
tgtgttgtgt tcacaagagc tattgcggat acatccccat atggatggag agctttgttt 2340
tggttcggtt caggtcctcc agttttgttt atcattttca gatacttttt accagaaact 2400
gaaacttacc ttgcttcaaa ggccagtcag gaagaggccg gaatcgagaa aaagttctgg 2460
aacggcatta aggtaacttt caaaaactat tggttgatgt tcatctactt agttattctg 2520
atggctggtt tcaatttcat gagtcatggt tcccaagatt tgtacccaac aatgctcaaa 2580
aaccagagac atttttcagc cgacagatca acagtcacta attgtgtggc aaatttcgga 2640
gcaattgcag gcggcatgtt aattggtcat ttttctaccg ctctcggtag acgtttgtct 2700
ataatgatct cttgtgttat aggtggtgct ttgatctacc cttgggcatt tgtcggtaac 2760
tccgctggga caaacgccgg agtattcttc ttacagttct ttgtccaagg tgcttggggg 2820
gttgttccaa tccacctatc agaattaagt ccacctgaat taagatcttc tatggtcgga 2880
atcgcttacc aaatgggtaa cttggcttct tctgcatcaa gtacaatcga atctaagata 2940
ggcgaaagat ttccattgaa aaatgctaag ggcgaattcg aaaaaggctt ctacgactac 3000
ggtaaggtta tggcaatctt catgggatgc gtattcgggt tcgttttgat tgttacattc 3060
gtaggaccag agaacagagg cgccactatg ttaacagagg atgcccaaat gatggttgat 3120
gctgaacata gacttgatgc ggaggagaag ggtgattttg aaaaccttga aagagttgac 3180
tctgaaggta agcagatgga taactttgtg gaagaggtgg cagaacctga aggtgtctac 3240
acaggctcac accctccaca atatgattca ccttacgaat ctaagtagtt aattaattta 3300
ccagcttact atccttcttg aaaatatgca ctctatatct tttagttctt aattgcaaca 3360
catagatttg ctgtataacg aattttatgc tattttttta atttggagtt cggtgatgaa 3420
agtgtcacag cgaatttcct cacatgtagg gaccgaattg tttacaagtt ctctgtacca 3480
ccatggagac atcaaagatt gaaaatctat ggaaagatat ggacggtagc aacaagaata 3540
tagcacgagc cgcggagttc atttcgttac ttttgatatc gctcacaact attgcgaagc 3600
gcttcagtga aaaaatcata aggaaaagtt gtaaatatta ttggtagtat tcgtttggta 3660
aagtagaggg ggtaattttt cccctttatt ttgttcatac attcttaaat tgctttgcct 3720
ctccttttgg aaagctatac ttcggagcac tgttgagcga aggctcaggc cggcatatga 3780
cgttttatta cctttgatca catttccacg ccatttcgca ttctcaccct cataagtcat 3840
acaccgaaaa gaaagtttaa gggatcaatg agcttactat aatctcagta tatttatttt 3900
tatcgatgat tcaccacaac aatcttgctc ccgaaaagaa agcagacgga gtagaagcat 3960
ttgaaactcc ttcagacctt caagtatata tatatatata tatatatgta tatgtgtaca 4020
ttttcacgct aatactaatg tataattaga agataatttt tactcatttt tcgttatctt 4080
cacgtcaccc gaacctagaa ccaaatgtca ttttcacgat atgtaaatag tgaaataggc 4140
aaaaacgcca aaaagtagta agcgcaacat acactaaacc attaaagaat atctcgacca 4200
gaatctaaca gatatacatg ttccgataat gtctgagtta ggtgagtatt ctaaattaga 4260
aaacaaagag cttagaacgg agtttgaatt gacaaatttt ccttttccag gcacaactga 4320
taacgactcc gatgacggaa gccaagggca gaactctttg aatatcatta ctcctgacat 4380
ggatgatact ctggttaatg atgtacttcg agaaaacgat aaaaagtcta gtatgagaat 4440
ggcttttatg aatctagcaa actctattct tggtgccgga ataattactc agccgttcgc 4500
gatcaaaaat gctggtatat taggcgggct attatcatac gtagccctcg gatttatagt 4560
tgattggacg ttaagactta ttgtcattaa cttgactctt gctggcaaga gaacatacca 4620
gggtacggtc gaacatgtaa tgggtaaaaa agggaaattg ctgattctat ttacaaacgg 4680
gttatttgca tttggtggat gtattgg 4707
<210> 80
<211> 7332
<212> DNA
<213> 酿酒酵母(Saccharomyces cerevisiae)
<400> 80
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agtttaaacg gccagaaaaa ggaagtgttt ccctccttct tgaattgatg 900
ttaccctcat aaagcacgtg gcctcttatc gagaaagaaa ttaccgtcgc tcgtgatttg 960
tttgcaaaaa gaacaaaact gaaaaaaccc agacacgctc gacttcctgt cttcctgttg 1020
attgcagctt ccaatttcgt cacacaacaa ggtcctagcg acggctcaca ggttttgtaa 1080
caagcaatcg aaggttctgg aatggcggga aagggtttag taccacatgc tatgatgccc 1140
actgtgatct ccagagcaaa gttcgttcga tcgtactgtt actctctctc tttcaaacag 1200
aattgtccga atcgtgtgac aacaacagcc tgttctcaca cactcttttc ttctaaccaa 1260
gggggtggtt tagtttagta gaacctcgtg aaacttacat ttacatatat ataaacttgc 1320
ataaattggt caatgcaaga aatacatatt tggtcttttc taattcgtag tttttcaagt 1380
tcttagatgc tttctttttc tcttttttac agatcatcaa ggaagtaatt atttttacaa 1440
gtctaggcta gcatgttgtg gaagcgtact tgcacaaggc taataaagcc tattgcacaa 1500
cctagaggaa ggctggtgag aagatcatgc tacagatacg cctcaacagg cacaggcagc 1560
accgacagca gcagtcagtg gttaaaatac tctgtcatcg cctcttcagc tactctattc 1620
ggttatttgt tcgctaagaa cctctattct agggagacta aggaggacct gatagagaaa 1680
ctggaaatgg tcaaaaagat cgacccagta aattctacgt taaagctgtc ctcattggac 1740
tcaccagact atttgcacga cccggccaag atcgataagg ttgttgagga cctgaagcag 1800
gtgctgggaa acaagcctga aaactactct gatgcgaaat ccgatttgga cgcccattca 1860
gatacctact tcaacacgca tcacccctct ctcgagcaaa gacctaggat tatattattc 1920
cctcatacta ccgaagaagt ttccaaaatt ttgaaaatat gtcacgataa caacatgcca 1980
gttgtaccct tctcgggcgg aacgtccttg gaggggcact tcctgcctac aagaattgga 2040
gataccataa ccgtagacct gtccaagttt atgaataacg tcgtaaaatt tgacaagctg 2100
gacctggaca tcaccgtgca ggccggtcta ccctgggagg atttgaatga ctatttgagc 2160
gaccacggtt tgatgtttgg ctgtgaccct ggtccaggtg cacagattgg tggttgcatt 2220
gctaattctt gttcaggaac caacgcctac cgttacggta ccatgaagga gaatattata 2280
aacatgacta tagtgttgcc ggacgggacc atcgtcaaga cgaagaaaag acccagaaag 2340
tcgagcgctg gctataactt aaatgggtta tttgtgggaa gtgaaggtac cttaggtatt 2400
gttactgaag ctactgtcaa gtgtcatgtc aagcccaaag ctgaaactgt tgcggtggta 2460
tcctttgata ctatcaagga tgcggccgca tgtgcttcta atctgactca gagtggtatt 2520
catttgaacg ccatggagtt actggatgaa aatatgatga agttgatcaa cgcatctgaa 2580
tccacggaca gatgtgattg ggtagagaaa ccaactatgt ttttcaagat tggtgggaga 2640
tctcccaaca ttgtcaatgc tcttgtggat gaagttaagg ctgtcgccca gttaaatcac 2700
tgcaacagtt ttcagtttgc taaagatgat gacgaaaaat tggaattatg ggaagctaga 2760
aaggtcgcgc tatggtctgt gctagacgct gataagagca aagacaaatc tgctaaaatt 2820
tggacaactg atgtagctgt tcctgtgtcg cagttcgaca aggttattca cgaaactaaa 2880
aaggacatgc aagctagtaa gctgatcaac gccattgttg gtcatgcagg tgatggtaac 2940
ttccatgcat tcatcgtcta cagaacccct gaagaacacg aaacctgtag ccaacttgtt 3000
gacagaatgg tcaagagagc actggacgca gaaggcactt gcacgggtga acacggtgtt 3060
ggtattggta aaagagagta cttgctcgaa gaattaggtg aagcacccgt cgatttgatg 3120
agaaagatta agctagctat tgacccaaag agaatcatga acccggacaa aatctttaaa 3180
actgatccaa acgagcccgc taatgattac aggtgagtcg actaagaagt tttgttaaaa 3240
ataaatcatt ttttaattga gcattcttat tcctatttta tttaaatagt tttatgtatt 3300
gttagctaca tacaacagtt taaatcaaat tttctttttc ccaagtccaa aatggaggtt 3360
tattttgatg acccgcatgc gattatgttt tgaaagtata agactacata catgtacata 3420
tatttaaaca tgtaaacccg tccattatat tgccgggcag accccgggtc gagatctccc 3480
gagatctccc gagtttatca ttatcaatac tgccatttca aagaatacgt aaataattaa 3540
tagtagtgat tttcctaact ttatttagtc aaaaaattgg ccttttaatt ctgctgtaac 3600
ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcat aggtgtctgg 3660
gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt ttaagctggc 3720
atccagaaaa aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa ccatcagttc 3780
ataggtccat tctcttagcg caactacaca gaacaggggc acaaacaggc aaaaaacggg 3840
cacaacctca atggagtgat gcaacctgct tggagtaaat gatgacacaa ggcaattgac 3900
ctacgcatgt atctatctca ttttcttaca ccttctatta ccttctgctc tctctgattt 3960
ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa attattcccc tatttgacta 4020
ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt cttaaacttc 4080
ttaaattcta cttttatagt tagtcttttt tttagtttaa aacaccaaga acttagtttc 4140
gaataaacac acataaacaa acaaatctag aatgctaaaa tacaaacctt tactaaaaat 4200
ctcgaagaac tgtgaggctg ctatcctcag agcgtctaag actagattga acacaatccg 4260
cgcgtacggt tctaccgttc caaaatccaa gtcgttcgaa caagactcaa gaaaacgcac 4320
acagtcatgg actgccttga gagtcggtgc aattctagcc gctactagtt ccgtggcgta 4380
tctaaactgg cataatggcc aaatagacaa cgagccgaaa ctggatatga ataaacaaaa 4440
gatttcgccc gctgaagttg ccaagcataa caagcccgat gattgttggg ttgtgatcaa 4500
tggttacgta tacgacttaa cgcgattcct accaaatcat ccaggtgggc aggatgttat 4560
caagtttaac gccgggaaag atgtcactgc tatttttgaa ccactacatg ctcctaatgt 4620
catcgataag tatatagctc ccgagaaaaa attgggtccc cttcaaggat ccatgcctcc 4680
tgaacttgtc tgtcctcctt atgctcctgg tgaaactaag gaagatatcg ctagaaaaga 4740
acaactaaaa tcgctgctac ctcctctaga taatattatt aacctttacg actttgaata 4800
cttggcctct caaactttga ctaaacaagc gtgggcctac tattcctccg gtgctaacga 4860
cgaagttact cacagagaaa accataatgc ttatcatagg atttttttca aaccaaagat 4920
ccttgtagat gtacgcaaag tagacatttc aactgacatg ttgggttctc atgtggatgt 4980
tcccttctac gtgtctgcta cagctttgtg taaactggga aaccccttag aaggtgaaaa 5040
agatgtcgcc agaggttgtg gccaaggtgt gacaaaagtc ccacaaatga tatctacttt 5100
ggcttcatgt tcccctgagg aaattattga agcagcaccc tctgataaac aaattcaatg 5160
gtaccaacta tatgttaact ctgatagaaa gatcactgat gatttggtta aaaatgtaga 5220
aaagctgggt gtaaaggcat tatttgtcac tgtggatgct ccaagtttag gtcaaagaga 5280
aaaagatatg aagctgaaat tttccaatac aaaggctggt ccaaaagcga tgaagaaaac 5340
taatgtagaa gaatctcaag gtgcttcgag agcgttatca aagtttattg acccctcttt 5400
gacttggaaa gatatagaag agttgaagaa aaagacaaaa ctacctattg ttatcaaagg 5460
tgttcaacgt accgaagatg ttatcaaagc agcagaaatc ggtgtaagtg gggtggttct 5520
atccaatcat ggtggtagac aattagattt ttcaagggct cccattgaag tcctggctga 5580
aaccatgcca atcctggaac aacgtaactt gaaggataag ttggaagttt tcgtggacgg 5640
tggtgttcgt cgtggtacag atgtcttgaa agcgttatgt ctaggtgcta aaggtgttgg 5700
tttgggtaga ccattcttgt atgcgaactc atgctatggt cgtaatggtg ttgaaaaagc 5760
cattgaaatt ttaagagatg aaattgaaat gtctatgaga ctattaggtg ttactagcat 5820
tgcggaattg aagcctgatc ttttagatct atcaacacta aaggcaagaa cagttggagt 5880
accaaacgac gtgctgtata atgaagttta tgagggacct actttaacag aatttgagga 5940
tgcatgatta attaaacagg ccccttttcc tttgtcgata tcatgtaatt agttatgtca 6000
cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg agttagacaa 6060
cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt 6120
atatttcaaa tttttctttt ttttctgtac aaacgcgtgt acgcatgtaa cgggcagacg 6180
gccggccata acttcgtata atgtatgcta tacgaagtta tggcaacggt tcatcatctc 6240
atggatctgc acatgaacaa acaccagagt caaacgacgt tgaaattgag gctactgcgc 6300
caattgatga caatacagac gatgataaca aaccgaagtt atctgatgta gaaaaggatt 6360
agagatgcta agagatagtg atgatatttc ataaataatg taattctata tatgttaatt 6420
accttttttg cgaggcatat ttatggtgaa ggataagttt tgaccatcaa agaaggttaa 6480
tgtggctgtg gtttcagggt ccataaagct tttcaattca tctttttttt ttttgttctt 6540
ttttttgatt ccggtttctt tgaaattttt ttgattcggt aatctccgag cagaaggaag 6600
aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtggt gttgaagaaa 6660
catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa 6720
gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg 6780
ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg 6840
ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac 6900
taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa 6960
aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg 7020
gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca 7080
ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcgg 7140
aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct 7200
ccctagctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt 7260
ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt 7320
tgattatgac ac 7332
<210> 81
<211> 7717
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和构巢曲霉。
<400> 81
tcctattcgg agatacaggc aacaagtgat agagggccca ttatgaatac gcacctctat 60
gtatttccga gatacgatta ctccagttcc tcttacaaga aatgcataaa aatagttaca 120
attaattaga acaagaactt atttagaaca cgttcacact gagtaagaac tcttgtccct 180
tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa aaaaagaaat aaaataacgg 240
caaacagcaa aggccacaga accgtattca tgttacttct gcaatatcaa tcacttactg 300
gcaagtgcgt ataaattaaa cctatttctt tatcatcata tttacttata tctttaacag 360
attccaaacc ctaaagtgtc cgaattttca atagggcgaa cttgaagaat aaccaaggtc 420
aataatatat cttttagtat aaccctgaaa tttgccctat agaaatctag ggtttctgtg 480
tggtttccgg gtgagtcata cggctttttt gaatttcttt ttttgcagtt gtctctatca 540
atgaaaattt cgaggaagac gataaggtta agataagtag ataagagaat gatacgagat 600
aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga gtaaaccgaa tcagggaatc 660
cctttttgca aaaacatcaa ttatcctttt ctttttttta cgtatatatc tggaacagaa 720
atatataagt tactattata cttatagttg gatccagttt ttaatctgtc gtcaatcgaa 780
agtttatttc agagttcttc agacttctta actcctgtaa aaacaaaaaa aaaaaaaggc 840
atagcactcg agtttaaacg gccagaaaaa ggaagtgttt ccctccttct tgaattgatg 900
ttaccctcat aaagcacgtg gcctcttatc gagaaagaaa ttaccgtcgc tcgtgatttg 960
tttgcaaaaa gaacaaaact gaaaaaaccc agacacgctc gacttcctgt cttcctgttg 1020
attgcagctt ccaatttcgt cacacaacaa ggtcctagcg acggctcaca ggttttgtaa 1080
caagcaatcg aaggttctgg aatggcggga aagggtttag taccacatgc tatgatgccc 1140
actgtgatct ccagagcaaa gttcgttcga tcgtactgtt actctctctc tttcaaacag 1200
aattgtccga atcgtgtgac aacaacagcc tgttctcaca cactcttttc ttctaaccaa 1260
gggggtggtt tagtttagta gaacctcgtg aaacttacat ttacatatat ataaacttgc 1320
ataaattggt caatgcaaga aatacatatt tggtcttttc taattcgtag tttttcaagt 1380
tcttagatgc tttctttttc tcttttttac agatcatcaa ggaagtaatt atttttacaa 1440
gtctaggcta gcatgttgtg gaagcgtact tgcacaaggc taataaagcc tattgcacaa 1500
cctagaggaa ggctggtgag aagatcatgc tacagatacg cctcaacagg cacaggcagc 1560
accgacagca gcagtcagtg gttaaaatac tctgtcatcg cctcttcagc tactctattc 1620
ggttatttgt tcgctaagaa cctctattct agggagacta aggaggacct gatagagaaa 1680
ctggaaatgg tcaaaaagat cgacccagta aattctacgt taaagctgtc ctcattggac 1740
tcaccagact atttgcacga cccggccaag atcgataagg ttgttgagga cctgaagcag 1800
gtgctgggaa acaagcctga aaactactct gatgcgaaat ccgatttgga cgcccattca 1860
gatacctact tcaacacgca tcacccctct ctcgagcaaa gacctaggat tatattattc 1920
cctcatacta ccgaagaagt ttccaaaatt ttgaaaatat gtcacgataa caacatgcca 1980
gttgtaccct tctcgggcgg aacgtccttg gaggggcact tcctgcctac aagaattgga 2040
gataccataa ccgtagacct gtccaagttt atgaataacg tcgtaaaatt tgacaagctg 2100
gacctggaca tcaccgtgca ggccggtcta ccctgggagg atttgaatga ctatttgagc 2160
gaccacggtt tgatgtttgg ctgtgaccct ggtccaggtg cacagattgg tggttgcatt 2220
gctaattctt gttcaggaac caacgcctac cgttacggta ccatgaagga gaatattata 2280
aacatgacta tagtgttgcc ggacgggacc atcgtcaaga cgaagaaaag acccagaaag 2340
tcgagcgctg gctataactt aaatgggtta tttgtgggaa gtgaaggtac cttaggtatt 2400
gttactgaag ctactgtcaa gtgtcatgtc aagcccaaag ctgaaactgt tgcggtggta 2460
tcctttgata ctatcaagga tgcggccgca tgtgcttcta atctgactca gagtggtatt 2520
catttgaacg ccatggagtt actggatgaa aatatgatga agttgatcaa cgcatctgaa 2580
tccacggaca gatgtgattg ggtagagaaa ccaactatgt ttttcaagat tggtgggaga 2640
tctcccaaca ttgtcaatgc tcttgtggat gaagttaagg ctgtcgccca gttaaatcac 2700
tgcaacagtt ttcagtttgc taaagatgat gacgaaaaat tggaattatg ggaagctaga 2760
aaggtcgcgc tatggtctgt gctagacgct gataagagca aagacaaatc tgctaaaatt 2820
tggacaactg atgtagctgt tcctgtgtcg cagttcgaca aggttattca cgaaactaaa 2880
aaggacatgc aagctagtaa gctgatcaac gccattgttg gtcatgcagg tgatggtaac 2940
ttccatgcat tcatcgtcta cagaacccct gaagaacacg aaacctgtag ccaacttgtt 3000
gacagaatgg tcaagagagc actggacgca gaaggcactt gcacgggtga acacggtgtt 3060
ggtattggta aaagagagta cttgctcgaa gaattaggtg aagcacccgt cgatttgatg 3120
agaaagatta agctagctat tgacccaaag agaatcatga acccggacaa aatctttaaa 3180
actgatccaa acgagcccgc taatgattac aggtgagtcg actaagaagt tttgttaaaa 3240
ataaatcatt ttttaattga gcattcttat tcctatttta tttaaatagt tttatgtatt 3300
gttagctaca tacaacagtt taaatcaaat tttctttttc ccaagtccaa aatggaggtt 3360
tattttgatg acccgcatgc gattatgttt tgaaagtata agactacata catgtacata 3420
tatttaaaca tgtaaacccg tccattatat tgccgggcag accccgggtc gagatctccc 3480
gagatctccc gagtttatca ttatcaatac tgccatttca aagaatacgt aaataattaa 3540
tagtagtgat tttcctaact ttatttagtc aaaaaattgg ccttttaatt ctgctgtaac 3600
ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcat aggtgtctgg 3660
gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt ttaagctggc 3720
atccagaaaa aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa ccatcagttc 3780
ataggtccat tctcttagcg caactacaca gaacaggggc acaaacaggc aaaaaacggg 3840
cacaacctca atggagtgat gcaacctgct tggagtaaat gatgacacaa ggcaattgac 3900
ctacgcatgt atctatctca ttttcttaca ccttctatta ccttctgctc tctctgattt 3960
ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa attattcccc tatttgacta 4020
ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt cttaaacttc 4080
ttaaattcta cttttatagt tagtcttttt tttagtttaa aacaccaaga acttagtttc 4140
gaataaacac acataaacaa acaaatctag aatgctaaaa tacaaacctt tactaaaaat 4200
ctcgaagaac tgtgaggctg ctatcctcag agcgtctaag actagattga acacaatccg 4260
cgcgtacggt tctaccgttc caaaatccaa gtcgttcgaa caagactcaa gaaaacgcac 4320
acagtcatgg actgccttga gagtcggtgc aattctagcc gctactagtt ccgtggcgta 4380
tctaaactgg cataatggcc aaatagacaa cgagccgaaa ctggatatga ataaacaaaa 4440
gatttcgccc gctgaagttg ccaagcataa caagcccgat gattgttggg ttgtgatcaa 4500
tggttacgta tacgacttaa cgcgattcct accaaatcat ccaggtgggc aggatgttat 4560
caagtttaac gccgggaaag atgtcactgc tatttttgaa ccactacatg ctcctaatgt 4620
catcgataag tatatagctc ccgagaaaaa attgggtccc cttcaaggat ccatgcctcc 4680
tgaacttgtc tgtcctcctt atgctcctgg tgaaactaag gaagatatcg ctagaaaaga 4740
acaactaaaa tcgctgctac ctcctctaga taatattatt aacctttacg actttgaata 4800
cttggcctct caaactttga ctaaacaagc gtgggcctac tattcctccg gtgctaacga 4860
cgaagttact cacagagaaa accataatgc ttatcatagg atttttttca aaccaaagat 4920
ccttgtagat gtacgcaaag tagacatttc aactgacatg ttgggttctc atgtggatgt 4980
tcccttctac gtgtctgcta cagctttgtg taaactggga aaccccttag aaggtgaaaa 5040
agatgtcgcc agaggttgtg gccaaggtgt gacaaaagtc ccacaaatga tatctacttt 5100
ggcttcatgt tcccctgagg aaattattga agcagcaccc tctgataaac aaattcaatg 5160
gtaccaacta tatgttaact ctgatagaaa gatcactgat gatttggtta aaaatgtaga 5220
aaagctgggt gtaaaggcat tatttgtcac tgtggatgct ccaagtttag gtcaaagaga 5280
aaaagatatg aagctgaaat tttccaatac aaaggctggt ccaaaagcga tgaagaaaac 5340
taatgtagaa gaatctcaag gtgcttcgag agcgttatca aagtttattg acccctcttt 5400
gacttggaaa gatatagaag agttgaagaa aaagacaaaa ctacctattg ttatcaaagg 5460
tgttcaacgt accgaagatg ttatcaaagc agcagaaatc ggtgtaagtg gggtggttct 5520
atccaatcat ggtggtagac aattagattt ttcaagggct cccattgaag tcctggctga 5580
aaccatgcca atcctggaac aacgtaactt gaaggataag ttggaagttt tcgtggacgg 5640
tggtgttcgt cgtggtacag atgtcttgaa agcgttatgt ctaggtgcta aaggtgttgg 5700
tttgggtaga ccattcttgt atgcgaactc atgctatggt cgtaatggtg ttgaaaaagc 5760
cattgaaatt ttaagagatg aaattgaaat gtctatgaga ctattaggtg ttactagcat 5820
tgcggaattg aagcctgatc ttttagatct atcaacacta aaggcaagaa cagttggagt 5880
accaaacgac gtgctgtata atgaagttta tgagggacct actttaacag aatttgagga 5940
tgcatgatta attaaacagg ccccttttcc tttgtcgata tcatgtaatt agttatgtca 6000
cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg agttagacaa 6060
cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt 6120
atatttcaaa tttttctttt ttttctgtac aaacgcgtgt acgcatgtaa cgggcagacg 6180
gccggccata acttcgtata atgtatgcta tacgaagtta tccttacatc acacccaatc 6240
ccccacaagt gatcccccac acaccatagc ttcaaaatgt ttctactcct tttttactct 6300
tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat 6360
actaaatttc ccctctttct tcctctaggg tggcgttaat tacccgtact aaaggtttgg 6420
aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt 6480
tatcacgttt ctttttcttg aaaaattttt tttttgattt ttttctcttt cgatgacctc 6540
ccattgatat ttaagttaat aaatggtctt caatttctca agtttcagtt tcgtttttct 6600
tgttctatta caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc 6660
taagttttaa ttacaaaatg ccacaatcct gggaagaatt ggccgccgac aaacgtgccc 6720
gtttggctaa aaccattcct gacgaatgga aggttcaaac tttgcctgcc gaagattccg 6780
ttattgattt cccaaagaag tccggtattt tgtctgaggc tgaattgaag attaccgaag 6840
cctctgctgc tgatttggtc tccaagttgg ccgctggtga gttgacttct gttgaagtca 6900
ctttggcttt ttgtaagaga gctgctattg ctcaacaatt aaccaactgt gctcacgaat 6960
tcttcccaga tgctgcttta gctcaagcta gagaattaga tgaatactac gctaagcata 7020
agagaccagt tggtccatta cacggtttac caatctcttt aaaggaccaa ttgcgtgtta 7080
agggttacga aacctccatg ggttacattt cctggttaaa caaatacgat gaaggtgatt 7140
ccgtcttaac caccatgttg agaaaagctg gtgctgtttt ctacgttaag acctctgtcc 7200
cacaaacctt gatggtctgt gaaaccgtca acaacatcat tggtagaact gtcaatccaa 7260
gaaacaaaaa ttggtcctgt ggtggttctt ctggtggtga aggtgctatt gttggtatta 7320
gaggtggtgt tattggtgtc ggtactgaca ttggtggttc cattagagtc ccagctgctt 7380
tcaacttttt atacggtttg agaccatctc acggtagatt gccatatgct aaaatggcta 7440
actctatgga aggtcaagaa accgttcact ccgtcgttgg tcctatcact cactccgtcg 7500
aagacttgag attgttcacc aaatctgtct tgggtcaaga accttggaag tacgactcta 7560
aggtcatccc catgccatgg agacaatctg aatctgacat cattgcctct aagattaaga 7620
atggtggttt gaacattggt tattacaatt tcgacggtaa cgtcttgcca cacccaccaa 7680
ttttacgtgg tgtcgaaact accgttgccg ctttggc 7717
<210> 82
<211> 4983
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和贝酵母
<400> 82
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
gatctcccct aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata 1020
tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt 1080
ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag 1140
acataatggg ctaaacaaga ctacaccatt tacactgcct cattgatggt ggtacataac 1200
gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt 1260
tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt tttttttttt 1320
ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga 1380
cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 1440
atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 1500
ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt 1560
ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc 1620
attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca 1680
agcatacaat caactatctc atatacatct agaatgtcgt caacaacaga ggacaaaaat 1740
tccaatgaac aacagcaacc cgctgccaga aagctatact acaacacaag cacgtttgcc 1800
gagccacccc tggtcgatga agccggtaac cccataaact acgaaccgga agtctacaac 1860
ccggaccacg aaaagctgta ccacaatcca tcgctgcccg tgacctcaat tcaggacact 1920
agagatgacg agttactgga aagagtttac agtgtcgacc aagaagtaga atacgaagag 1980
gacgaagcag acaaaccgaa tctgagtctc gcatccataa aaaattatgc tttgacaaga 2040
ttcacgtctt tgttgcgcgt tcacgaggtc tccttgacaa acatcaaccc gatcccggaa 2100
ttgaggaaga tgacgttgca aaattggaac tattttttca tggggtattt agcctggttg 2160
acagccgcct ggtgtttctt ttgtgtttca gtgtcagtag ctccgctatc tgaactgtat 2220
gatagaccca ccaaggatat aacctggggg ttggggctgg tgttgttcgt tcgctcagcc 2280
ggtgcgatta tattcggttt atggacagat aaggcttcca gaaaatggcc ctacatcacc 2340
tgtttgtttt tatttgtcat tgcgcagctt tgtactccat ggtgtgacac ttaccaaaaa 2400
tttctgggcg ttagatggat tacaggtata gccatgggtg ggatttatgg gtgtgcttct 2460
gcaacagcca tagaagatgc tccagtgaaa gcacgttcat tcctatcagg tctctttttt 2520
tctgcatatg ccatggggtt tatcttcgca atcatttttt acagagcctt tggctacttc 2580
agggaagacg gctggaaaat attgttctgg tttagcattt tcttaccaat cttactaatt 2640
ttctggagat tactatggcc tgaaacaaaa tatttcacca aagtcttgaa ggctcgtaga 2700
ctaatactaa gtgacgctgt gaaagccaac ggtggcgaac ctctgcccaa ggccaatttc 2760
aaacaaaaga tggtgtctgt gaaaaaaact gtacaaaaat actggctgtt gtttgcctat 2820
ctagtcatta tgttggttgg tccaaattac ttgacccacg cctctcagga tttgttgcca 2880
accatgctgc gtgcccaact agggttgtcc aaggatgccg tcactgttat tgtggttgtc 2940
accaacatcg gtgctatctg tggtggtatg atatttggtc aactaatgga agttactggt 3000
agaagattag gcctgttgat tgcgtgcgta atgggaggtt gttttaccta tcctgcattt 3060
atgttgagaa gcgagaaagc tattctggga gctggattca tgttatactt ttgtgtgttt 3120
ggtgtctggg gtattttgcc aatccacctg gctgaactgg ctcctgccga cgcaagggct 3180
ttggtcgctg gtttatccta ccaattgggt aatttggcct cagcagcagc ctctacaatt 3240
gagacggaat tagcaggaaa gtatccatta gaggtggatt cctctggtac cgttataaag 3300
gaagattacg cgaaagttat ggccatccta actggggccg ttttcatctt tactttttgt 3360
tgtgtctttg tagggcatga aaaattccat cgcgatctat cttctccagt tatgaagaaa 3420
tacataactc aagtggaagt ttacgaagct gatggtgttt caatcagtga aattgtggaa 3480
caaaagacag aatacggttc tgtgcaaatg gttgattcca atatctcaaa gacatttgaa 3540
gaacatgttg aaactgtttg attattaatt aatttaccag cttactatcc ttcttgaaaa 3600
tatgcactct atatctttta gttcttaatt gcaacacata gatttgctgt ataacgaatt 3660
ttatgctatt tttttaattt ggagttcggt gatgaaagtg tcacagcgaa tttcctcaca 3720
tgtagggacc gaattgttta caagttctct gtaccaccat ggagacatca aagattgaaa 3780
atctatggaa agatatggac ggtagcaaca agaatatagc acgagccgcg gagttcattt 3840
cgttactttt gatatcgctc acaactattg cgaagcgctt cagtgaaaaa atcataagga 3900
aaagttgtaa atattattgg tagtattcgt ttggtaaagt agagggggta atttttcccc 3960
tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag ctatacttcg 4020
gagcactgtt gagcgaaggc tcaggccggc atatgacgtt ttattacctt tgatcacatt 4080
tccacgccat ttcgcattct caccctcata agtcatacac cgaaaagaaa gtttaaggga 4140
tcaatgagct tactataatc tcagtatatt tatttttatc gatgattcac cacaacaatc 4200
ttgctcccga aaagaaagca gacggagtag aagcatttga aactccttca gaccttcaag 4260
tatatatata tatatatata tatgtatatg tgtacatttt cacgctaata ctaatgtata 4320
attagaagat aatttttact catttttcgt tatcttcacg tcacccgaac ctagaaccaa 4380
atgtcatttt cacgatatgt aaatagtgaa ataggcaaaa acgccaaaaa gtagtaagcg 4440
caacatacac taaaccatta aagaatatct cgaccagaat ctaacagata tacatgttcc 4500
gataatgtct gagttaggtg agtattctaa attagaaaac aaagagctta gaacggagtt 4560
tgaattgaca aattttcctt ttccaggcac aactgataac gactccgatg acggaagcca 4620
agggcagaac tctttgaata tcattactcc tgacatggat gatactctgg ttaatgatgt 4680
acttcgagaa aacgataaaa agtctagtat gagaatggct tttatgaatc tagcaaactc 4740
tattcttggt gccggaataa ttactcagcc gttcgcgatc aaaaatgctg gtatattagg 4800
cgggctatta tcatacgtag ccctcggatt tatagttgat tggacgttaa gacttattgt 4860
cattaacttg actcttgctg gcaagagaac ataccagggt acggtcgaac atgtaatggg 4920
taaaaaaggg aaattgctga ttctatttac aaacgggtta tttgcatttg gtggatgtat 4980
tgg 4983
<210> 83
<211> 615
<212> PRT
<213> 贝酵母(Saccharomyces bayanus)
<400> 83
Met Ser Ser Thr Thr Glu Asp Lys Asn Ser Asn Glu Gln Gln Gln Pro
1 5 10 15
Ala Ala Arg Lys Leu Tyr Tyr Asn Thr Ser Thr Phe Ala Glu Pro Pro
20 25 30
Leu Val Asp Glu Ala Gly Asn Pro Ile Asn Tyr Glu Pro Glu Val Tyr
35 40 45
Asn Pro Asp His Glu Lys Leu Tyr His Asn Pro Ser Leu Pro Val Thr
50 55 60
Ser Ile Gln Asp Thr Arg Asp Asp Glu Leu Leu Glu Arg Val Tyr Ser
65 70 75 80
Val Asp Gln Glu Val Glu Tyr Glu Glu Asp Glu Ala Asp Lys Pro Asn
85 90 95
Leu Ser Leu Ala Ser Ile Lys Asn Tyr Ala Leu Thr Arg Phe Thr Ser
100 105 110
Leu Leu Arg Val His Glu Val Ser Leu Thr Asn Ile Asn Pro Ile Pro
115 120 125
Glu Leu Arg Lys Met Thr Leu Gln Asn Trp Asn Tyr Phe Phe Met Gly
130 135 140
Tyr Leu Ala Trp Leu Thr Ala Ala Trp Cys Phe Phe Cys Val Ser Val
145 150 155 160
Ser Val Ala Pro Leu Ser Glu Leu Tyr Asp Arg Pro Thr Lys Asp Ile
165 170 175
Thr Trp Gly Leu Gly Leu Val Leu Phe Val Arg Ser Ala Gly Ala Ile
180 185 190
Ile Phe Gly Leu Trp Thr Asp Lys Ala Ser Arg Lys Trp Pro Tyr Ile
195 200 205
Thr Cys Leu Phe Leu Phe Val Ile Ala Gln Leu Cys Thr Pro Trp Cys
210 215 220
Asp Thr Tyr Gln Lys Phe Leu Gly Val Arg Trp Ile Thr Gly Ile Ala
225 230 235 240
Met Gly Gly Ile Tyr Gly Cys Ala Ser Ala Thr Ala Ile Glu Asp Ala
245 250 255
Pro Val Lys Ala Arg Ser Phe Leu Ser Gly Leu Phe Phe Ser Ala Tyr
260 265 270
Ala Met Gly Phe Ile Phe Ala Ile Ile Phe Tyr Arg Ala Phe Gly Tyr
275 280 285
Phe Arg Glu Asp Gly Trp Lys Ile Leu Phe Trp Phe Ser Ile Phe Leu
290 295 300
Pro Ile Leu Leu Ile Phe Trp Arg Leu Leu Trp Pro Glu Thr Lys Tyr
305 310 315 320
Phe Thr Lys Val Leu Lys Ala Arg Arg Leu Ile Leu Ser Asp Ala Val
325 330 335
Lys Ala Asn Gly Gly Glu Pro Leu Pro Lys Ala Asn Phe Lys Gln Lys
340 345 350
Met Val Ser Val Lys Lys Thr Val Gln Lys Tyr Trp Leu Leu Phe Ala
355 360 365
Tyr Leu Val Ile Met Leu Val Gly Pro Asn Tyr Leu Thr His Ala Ser
370 375 380
Gln Asp Leu Leu Pro Thr Met Leu Arg Ala Gln Leu Gly Leu Ser Lys
385 390 395 400
Asp Ala Val Thr Val Ile Val Val Val Thr Asn Ile Gly Ala Ile Cys
405 410 415
Gly Gly Met Ile Phe Gly Gln Leu Met Glu Val Thr Gly Arg Arg Leu
420 425 430
Gly Leu Leu Ile Ala Cys Val Met Gly Gly Cys Phe Thr Tyr Pro Ala
435 440 445
Phe Met Leu Arg Ser Glu Lys Ala Ile Leu Gly Ala Gly Phe Met Leu
450 455 460
Tyr Phe Cys Val Phe Gly Val Trp Gly Ile Leu Pro Ile His Leu Ala
465 470 475 480
Glu Leu Ala Pro Ala Asp Ala Arg Ala Leu Val Ala Gly Leu Ser Tyr
485 490 495
Gln Leu Gly Asn Leu Ala Ser Ala Ala Ala Ser Thr Ile Glu Thr Glu
500 505 510
Leu Ala Gly Lys Tyr Pro Leu Glu Val Asp Ser Ser Gly Thr Val Ile
515 520 525
Lys Glu Asp Tyr Ala Lys Val Met Ala Ile Leu Thr Gly Ala Val Phe
530 535 540
Ile Phe Thr Phe Cys Cys Val Phe Val Gly His Glu Lys Phe His Arg
545 550 555 560
Asp Leu Ser Ser Pro Val Met Lys Lys Tyr Ile Thr Gln Val Glu Val
565 570 575
Tyr Glu Ala Asp Gly Val Ser Ile Ser Glu Ile Val Glu Gln Lys Thr
580 585 590
Glu Tyr Gly Ser Val Gln Met Val Asp Ser Asn Ile Ser Lys Thr Phe
595 600 605
Glu Glu His Val Glu Thr Val
610 615
<210> 84
<211> 5192
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和乳酸克鲁维酵母
<400> 84
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
ggaagcccga aagagttatc gttactccga ttattttgta cagctgatgg gaccttgccg 1020
tcttcatttt tttttttttc acctatagag ccgggcagag ctgcccggct caactaaggg 1080
ccggaaaaaa aacggaaaaa agaaagccaa gcgtgtagac gtagtataac agtatatctg 1140
acacgcacgt gatgaccacg taatcgcatc gcccctcaca tctcacctct caccgctgac 1200
tcagcttcac taaaaaggaa aatatatact ctttcccagg caaggtgaca gcggtccccg 1260
tctcctccac aaaggcctct cctggggttt gagcaagtct aagtttacgt agcataaaaa 1320
ttctcggatt gcgtcaaata ataaaaaaag taactccact tctacttcta catcggaaaa 1380
acattccatt cacatatcgt ctttggccta tcttgttttg tccttggtag atcaggtcag 1440
tacaaacgca acacgaaaga acaaaaaaag aagaaaaaca gaaggccaag acagggtcaa 1500
tgagactgtt gtcctcctac tgtccctatg tctctggccg atcacgcgcc attgtccctc 1560
agaaacaaat caaacaccca caccccgggc acccaaagtc cccacccaca ccaccaatac 1620
gtaaacgggg cgccccctgc aggccctcct gcgcgcggcc tcccgccttg cttctctccc 1680
ctcccttttc tttttccagt tttccctatt ttgtcccttt ttccgcacaa caagtatcag 1740
aatgggttca tcaaatctat ccaacctaat tcgcacgtag actggcttgg tattggcagt 1800
ttcgcagtta tatatatact accatgagtg aaactgttac gttaccttaa attctttctc 1860
cctttaattt tcttttatct tactctccta cataagacat caagaaacaa ttgtatattg 1920
tacacccccc cccctccaca aacacaaata ttgataatat aaagtctaga atgaataaca 1980
acaacattac accagagtca gactcaatga agagtaacga taacgatcaa accaatgatt 2040
acatgccaga tgtggctgat ttcgatcata cacaaacaaa cacaaacgaa attgctagag 2100
caatttctca cccaggttct gttctgtcaa gagttgcaag ctacgtcagc agaaaggaca 2160
gatacgtaga cgagaatggc aatgaagttt ggcaagacga tgaagtgagt atcctaatgg 2220
aggaagatga gactcctgat ttcacatgga aaaacattag acattacgct atcactagat 2280
tcactacatt gacagaactt cacagagtgt ctatggaaaa catcaaccca attccagaat 2340
tgagaaaaat gacattacat aattggaact acttctttat ggggtatgcc gcgtggttgt 2400
gtgctgcttg ggcatttttc gccgtttccg ttagtacagc ccctcttgca accctttatg 2460
gcaaggaaac aaaagacatt tcatggggtc tatccttagt cttattcgtc agatctgctg 2520
gagcgattat ctttggcatc tggaccgaca attactccag aaagtggcca tacattacat 2580
gcttaggtct tttcttgatc tgtcagcttt gcacaccttg ggcaaagacc tacactcaat 2640
tcttgggagt tcgttggatt tccggtatcg caatgggtgg aatttacgcc tgtgcttctg 2700
caacagcgat tgaggatgca ccagtaaagg ctcgttcttt cttatccggc ttattcttca 2760
ctgcctacgc catgggtttt atcttcgcaa taatctttta cagagcattc ttaaacgtca 2820
acggtgaaaa ctactggaag gttcaattct ggttttcaat atggttacca gctgtactga 2880
tattgtggag actcgtatgg ccagaaacta agtacttcac caaagtacta aaagctagac 2940
aactaatgcg tgatgatgca atagccaaaa acggtggtca accactccca aagttgtctt 3000
tcaagcaaaa gttcgccaat gtgaaaaaga ccgttagtaa atactggctt ttgtttggtt 3060
acttaattct cttgttggtt ggtcctaact acctaacaca cgcctctcaa gacttgtttc 3120
ctacaatgtt aagagcacaa ttgagatttt ctgaagatgc tgtcaccgtg gccattgtag 3180
ttgtttgtct gggtagcatt gctgggggga tgtttttcgg acagttgatg gagatcactg 3240
gtagaagagt tggcctacta ttggccctaa tcatggctgg ctgctttact taccctgcct 3300
tcatgcttaa aacttcatct gcagtgctgg gggcaggctt tatgctctgg ttctcaatct 3360
taggtgtgtg gggtgtttta ccaatccatt tatcagaatt gtcccctcca gaagctagag 3420
cattagtctc tggattagca tatcagctgg gtaatctagc ttcagcagct tctgttgtta 3480
tagagaatga tttggcggat ttgtatccaa tagaatggaa ttctgctgga gaagttacaa 3540
acaaggacta ctctaaggta atggctattc ttacaggttc ttcagtcatt ttcactttcg 3600
tgttggtttt cgttggtcac gaaaagtttc atagagattt gtcatcccca catcttaagt 3660
catacatcga gagagtcgat caaacagaag aggtcgcagc tatgactgga tctactgcga 3720
actctatctc tagtaagcct tcagatgatc agctcgaaaa agtttcagtc tagttaatta 3780
atttaccagc ttactatcct tcttgaaaat atgcactcta tatcttttag ttcttaattg 3840
caacacatag atttgctgta taacgaattt tatgctattt ttttaatttg gagttcggtg 3900
atgaaagtgt cacagcgaat ttcctcacat gtagggaccg aattgtttac aagttctctg 3960
taccaccatg gagacatcaa agattgaaaa tctatggaaa gatatggacg gtagcaacaa 4020
gaatatagca cgagccgcgg agttcatttc gttacttttg atatcgctca caactattgc 4080
gaagcgcttc agtgaaaaaa tcataaggaa aagttgtaaa tattattggt agtattcgtt 4140
tggtaaagta gagggggtaa tttttcccct ttattttgtt catacattct taaattgctt 4200
tgcctctcct tttggaaagc tatacttcgg agcactgttg agcgaaggct caggccggca 4260
tatgacgttt tattaccttt gatcacattt ccacgccatt tcgcattctc accctcataa 4320
gtcatacacc gaaaagaaag tttaagggat caatgagctt actataatct cagtatattt 4380
atttttatcg atgattcacc acaacaatct tgctcccgaa aagaaagcag acggagtaga 4440
agcatttgaa actccttcag accttcaagt atatatatat atatatatat atgtatatgt 4500
gtacattttc acgctaatac taatgtataa ttagaagata atttttactc atttttcgtt 4560
atcttcacgt cacccgaacc tagaaccaaa tgtcattttc acgatatgta aatagtgaaa 4620
taggcaaaaa cgccaaaaag tagtaagcgc aacatacact aaaccattaa agaatatctc 4680
gaccagaatc taacagatat acatgttccg ataatgtctg agttaggtga gtattctaaa 4740
ttagaaaaca aagagcttag aacggagttt gaattgacaa attttccttt tccaggcaca 4800
actgataacg actccgatga cggaagccaa gggcagaact ctttgaatat cattactcct 4860
gacatggatg atactctggt taatgatgta cttcgagaaa acgataaaaa gtctagtatg 4920
agaatggctt ttatgaatct agcaaactct attcttggtg ccggaataat tactcagccg 4980
ttcgcgatca aaaatgctgg tatattaggc gggctattat catacgtagc cctcggattt 5040
atagttgatt ggacgttaag acttattgtc attaacttga ctcttgctgg caagagaaca 5100
taccagggta cggtcgaaca tgtaatgggt aaaaaaggga aattgctgat tctatttaca 5160
aacgggttat ttgcatttgg tggatgtatt gg 5192
<210> 85
<211> 4825
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和乳酸克鲁维酵母
<400> 85
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
ggaagagact aatcaaagaa tcgttttctc aaaaaattta atatcttaac tgatagtttg 1020
atcaaagggg caaaacgtag gggcaaacaa acggaaaaat cgtttctcaa attttctgat 1080
gccaagaact ctaaccagtc ttatctaaaa attgccttat gatccgtctc tccggttaca 1140
gcctgtgtaa ctgattaatc ctgcctttct aatcaccatt ctaatgtttt aattaaggga 1200
ttttgtcttc attaacggct ttcgctcata aaaatgttat gacgttttgc ccgcaggcgg 1260
gaaaccatcc acttcacgag actgatctcc tctgccggaa caccgggcat ctccaactta 1320
taagttggag aaataagaga atttcagatt gagagaatga aaaaaaaaaa aaaaaaggca 1380
gaggagagca taaaaatggg gttcactttt tggtaaagct atagcatgcc tatcacatat 1440
aaatagagtg ccagtagcga cttttttcac actcgaaata ctcttactac tgctctcttg 1500
ttgtttttat cacttcttgt ttcttcttgg taaatagaat atcaagctac aaaaagcata 1560
caatcaacta tcaactatta actatatcgt aatacactct agaatgaata acaacaacat 1620
tacaccagag tcagactcaa tgaagagtaa cgataacgat caaaccaatg attacatgcc 1680
agatgtggct gatttcgatc atacacaaac aaacacaaac gaaattgcta gagcaatttc 1740
tcacccaggt tctgttctgt caagagttgc aagctacgtc agcagaaagg acagatacgt 1800
agacgagaat ggcaatgaag tttggcaaga cgatgaagtg agtatcctaa tggaggaaga 1860
tgagactcct gatttcacat ggaaaaacat tagacattac gctatcacta gattcactac 1920
attgacagaa cttcacagag tgtctatgga aaacatcaac ccaattccag aattgagaaa 1980
aatgacatta cataattgga actacttctt tatggggtat gccgcgtggt tgtgtgctgc 2040
ttgggcattt ttcgccgttt ccgttagtac agcccctctt gcaacccttt atggcaagga 2100
aacaaaagac atttcatggg gtctatcctt agtcttattc gtcagatctg ctggagcgat 2160
tatctttggc atctggaccg acaattactc cagaaagtgg ccatacatta catgcttagg 2220
tcttttcttg atctgtcagc tttgcacacc ttgggcaaag acctacactc aattcttggg 2280
agttcgttgg atttccggta tcgcaatggg tggaatttac gcctgtgctt ctgcaacagc 2340
gattgaggat gcaccagtaa aggctcgttc tttcttatcc ggcttattct tcactgccta 2400
cgccatgggt tttatcttcg caataatctt ttacagagca ttcttaaacg tcaacggtga 2460
aaactactgg aaggttcaat tctggttttc aatatggtta ccagctgtac tgatattgtg 2520
gagactcgta tggccagaaa ctaagtactt caccaaagta ctaaaagcta gacaactaat 2580
gcgtgatgat gcaatagcca aaaacggtgg tcaaccactc ccaaagttgt ctttcaagca 2640
aaagttcgcc aatgtgaaaa agaccgttag taaatactgg cttttgtttg gttacttaat 2700
tctcttgttg gttggtccta actacctaac acacgcctct caagacttgt ttcctacaat 2760
gttaagagca caattgagat tttctgaaga tgctgtcacc gtggccattg tagttgtttg 2820
tctgggtagc attgctgggg ggatgttttt cggacagttg atggagatca ctggtagaag 2880
agttggccta ctattggccc taatcatggc tggctgcttt acttaccctg ccttcatgct 2940
taaaacttca tctgcagtgc tgggggcagg ctttatgctc tggttctcaa tcttaggtgt 3000
gtggggtgtt ttaccaatcc atttatcaga attgtcccct ccagaagcta gagcattagt 3060
ctctggatta gcatatcagc tgggtaatct agcttcagca gcttctgttg ttatagagaa 3120
tgatttggcg gatttgtatc caatagaatg gaattctgct ggagaagtta caaacaagga 3180
ctactctaag gtaatggcta ttcttacagg ttcttcagtc attttcactt tcgtgttggt 3240
tttcgttggt cacgaaaagt ttcatagaga tttgtcatcc ccacatctta agtcatacat 3300
cgagagagtc gatcaaacag aagaggtcgc agctatgact ggatctactg cgaactctat 3360
ctctagtaag ccttcagatg atcagctcga aaaagtttca gtctagttaa ttaatttacc 3420
agcttactat ccttcttgaa aatatgcact ctatatcttt tagttcttaa ttgcaacaca 3480
tagatttgct gtataacgaa ttttatgcta tttttttaat ttggagttcg gtgatgaaag 3540
tgtcacagcg aatttcctca catgtaggga ccgaattgtt tacaagttct ctgtaccacc 3600
atggagacat caaagattga aaatctatgg aaagatatgg acggtagcaa caagaatata 3660
gcacgagccg cggagttcat ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc 3720
ttcagtgaaa aaatcataag gaaaagttgt aaatattatt ggtagtattc gtttggtaaa 3780
gtagaggggg taatttttcc cctttatttt gttcatacat tcttaaattg ctttgcctct 3840
ccttttggaa agctatactt cggagcactg ttgagcgaag gctcaggccg gcatatgacg 3900
ttttattacc tttgatcaca tttccacgcc atttcgcatt ctcaccctca taagtcatac 3960
accgaaaaga aagtttaagg gatcaatgag cttactataa tctcagtata tttattttta 4020
tcgatgattc accacaacaa tcttgctccc gaaaagaaag cagacggagt agaagcattt 4080
gaaactcctt cagaccttca agtatatata tatatatata tatatgtata tgtgtacatt 4140
ttcacgctaa tactaatgta taattagaag ataattttta ctcatttttc gttatcttca 4200
cgtcacccga acctagaacc aaatgtcatt ttcacgatat gtaaatagtg aaataggcaa 4260
aaacgccaaa aagtagtaag cgcaacatac actaaaccat taaagaatat ctcgaccaga 4320
atctaacaga tatacatgtt ccgataatgt ctgagttagg tgagtattct aaattagaaa 4380
acaaagagct tagaacggag tttgaattga caaattttcc ttttccaggc acaactgata 4440
acgactccga tgacggaagc caagggcaga actctttgaa tatcattact cctgacatgg 4500
atgatactct ggttaatgat gtacttcgag aaaacgataa aaagtctagt atgagaatgg 4560
cttttatgaa tctagcaaac tctattcttg gtgccggaat aattactcag ccgttcgcga 4620
tcaaaaatgc tggtatatta ggcgggctat tatcatacgt agccctcgga tttatagttg 4680
attggacgtt aagacttatt gtcattaact tgactcttgc tggcaagaga acataccagg 4740
gtacggtcga acatgtaatg ggtaaaaaag ggaaattgct gattctattt acaaacgggt 4800
tatttgcatt tggtggatgt attgg 4825
<210> 86
<211> 5157
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母和乳酸克鲁维酵母
<400> 86
tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc 60
ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt 120
gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt 180
attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg 240
atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact 300
gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg 360
ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac 420
aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct 480
gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt 540
gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa 600
aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa 660
ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa 720
aaaattggaa agaaaaagct tcatggcctt tataaaaagg aaccatccaa tacctcgcca 780
gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag 840
atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa 900
atgaaggatt tcatgcgttt gataacttcg tataatgtat gctatacgaa gttatctcga 960
ggacaaccag gacgtaaagg gtagcctccc cataacataa actcaataaa atatatagtc 1020
ttcaacttga aaaaggaaca agctcatgca aagaggtggt acccgcacgc cgaaatgcat 1080
gcaagtaacc tattcaaagt aatatctcat acatgtttca tgagggtaac aacatgcgac 1140
tgggtgagca tatgttccgc tgatgtgatg tgcaagataa acaagcaaga cagaaactaa 1200
cttcttcttc atgtaataaa cacaccccgc gtttatttac ctatctttaa acttcaacac 1260
cttatatcat aactaatatt tcttgagata agcacactgc acccatacct tccttaaaaa 1320
cgtagcttcc agtttttggt ggttctggct tccttcccga ttccgcccgc taaacgcata 1380
attttgttgc ctggtggcat ttgcaaaatg cataacctat gcatttaaaa gattatgtat 1440
gctcttctga cttttcgtgt gatgaggctc gtggaaaaaa tgaataattt atgaatttga 1500
gaacaatttt gtgttgttac ggtattttac tatggaataa tcaatcaatt gaggatttta 1560
tgcaaatatc gtttgaatat ttttccgacc ctttgagtac ttttcttcat aattgcataa 1620
tattgtccgc tgcccgtttt tctgttagac ggtgtcttga tctacttgct atcgttcaac 1680
accaccttat tttctaacta tttttttttt agctcatttg aatcagctta tggtgatggc 1740
acatttttgc ataaacctag ctgtcctcgt tgaacatagg aaaaaaaaat atataaacaa 1800
ggctctttca ctctccttgg aatcagattt gggtttgttc cctttatttt catatttctt 1860
gtcatattct tttctcaatt attatcttct actcataacc tcacgcaaaa taacacagtc 1920
aaatcaatct ctagaatgaa taacaacaac attacaccag agtcagactc aatgaagagt 1980
aacgataacg atcaaaccaa tgattacatg ccagatgtgg ctgatttcga tcatacacaa 2040
acaaacacaa acgaaattgc tagagcaatt tctcacccag gttctgttct gtcaagagtt 2100
gcaagctacg tcagcagaaa ggacagatac gtagacgaga atggcaatga agtttggcaa 2160
gacgatgaag tgagtatcct aatggaggaa gatgagactc ctgatttcac atggaaaaac 2220
attagacatt acgctatcac tagattcact acattgacag aacttcacag agtgtctatg 2280
gaaaacatca acccaattcc agaattgaga aaaatgacat tacataattg gaactacttc 2340
tttatggggt atgccgcgtg gttgtgtgct gcttgggcat ttttcgccgt ttccgttagt 2400
acagcccctc ttgcaaccct ttatggcaag gaaacaaaag acatttcatg gggtctatcc 2460
ttagtcttat tcgtcagatc tgctggagcg attatctttg gcatctggac cgacaattac 2520
tccagaaagt ggccatacat tacatgctta ggtcttttct tgatctgtca gctttgcaca 2580
ccttgggcaa agacctacac tcaattcttg ggagttcgtt ggatttccgg tatcgcaatg 2640
ggtggaattt acgcctgtgc ttctgcaaca gcgattgagg atgcaccagt aaaggctcgt 2700
tctttcttat ccggcttatt cttcactgcc tacgccatgg gttttatctt cgcaataatc 2760
ttttacagag cattcttaaa cgtcaacggt gaaaactact ggaaggttca attctggttt 2820
tcaatatggt taccagctgt actgatattg tggagactcg tatggccaga aactaagtac 2880
ttcaccaaag tactaaaagc tagacaacta atgcgtgatg atgcaatagc caaaaacggt 2940
ggtcaaccac tcccaaagtt gtctttcaag caaaagttcg ccaatgtgaa aaagaccgtt 3000
agtaaatact ggcttttgtt tggttactta attctcttgt tggttggtcc taactaccta 3060
acacacgcct ctcaagactt gtttcctaca atgttaagag cacaattgag attttctgaa 3120
gatgctgtca ccgtggccat tgtagttgtt tgtctgggta gcattgctgg ggggatgttt 3180
ttcggacagt tgatggagat cactggtaga agagttggcc tactattggc cctaatcatg 3240
gctggctgct ttacttaccc tgccttcatg cttaaaactt catctgcagt gctgggggca 3300
ggctttatgc tctggttctc aatcttaggt gtgtggggtg ttttaccaat ccatttatca 3360
gaattgtccc ctccagaagc tagagcatta gtctctggat tagcatatca gctgggtaat 3420
ctagcttcag cagcttctgt tgttatagag aatgatttgg cggatttgta tccaatagaa 3480
tggaattctg ctggagaagt tacaaacaag gactactcta aggtaatggc tattcttaca 3540
ggttcttcag tcattttcac tttcgtgttg gttttcgttg gtcacgaaaa gtttcataga 3600
gatttgtcat ccccacatct taagtcatac atcgagagag tcgatcaaac agaagaggtc 3660
gcagctatga ctggatctac tgcgaactct atctctagta agccttcaga tgatcagctc 3720
gaaaaagttt cagtctagtt aattaattta ccagcttact atccttcttg aaaatatgca 3780
ctctatatct tttagttctt aattgcaaca catagatttg ctgtataacg aattttatgc 3840
tattttttta atttggagtt cggtgatgaa agtgtcacag cgaatttcct cacatgtagg 3900
gaccgaattg tttacaagtt ctctgtacca ccatggagac atcaaagatt gaaaatctat 3960
ggaaagatat ggacggtagc aacaagaata tagcacgagc cgcggagttc atttcgttac 4020
ttttgatatc gctcacaact attgcgaagc gcttcagtga aaaaatcata aggaaaagtt 4080
gtaaatatta ttggtagtat tcgtttggta aagtagaggg ggtaattttt cccctttatt 4140
ttgttcatac attcttaaat tgctttgcct ctccttttgg aaagctatac ttcggagcac 4200
tgttgagcga aggctcaggc cggcatatga cgttttatta cctttgatca catttccacg 4260
ccatttcgca ttctcaccct cataagtcat acaccgaaaa gaaagtttaa gggatcaatg 4320
agcttactat aatctcagta tatttatttt tatcgatgat tcaccacaac aatcttgctc 4380
ccgaaaagaa agcagacgga gtagaagcat ttgaaactcc ttcagacctt caagtatata 4440
tatatatata tatatatgta tatgtgtaca ttttcacgct aatactaatg tataattaga 4500
agataatttt tactcatttt tcgttatctt cacgtcaccc gaacctagaa ccaaatgtca 4560
ttttcacgat atgtaaatag tgaaataggc aaaaacgcca aaaagtagta agcgcaacat 4620
acactaaacc attaaagaat atctcgacca gaatctaaca gatatacatg ttccgataat 4680
gtctgagtta ggtgagtatt ctaaattaga aaacaaagag cttagaacgg agtttgaatt 4740
gacaaatttt ccttttccag gcacaactga taacgactcc gatgacggaa gccaagggca 4800
gaactctttg aatatcatta ctcctgacat ggatgatact ctggttaatg atgtacttcg 4860
agaaaacgat aaaaagtcta gtatgagaat ggcttttatg aatctagcaa actctattct 4920
tggtgccgga ataattactc agccgttcgc gatcaaaaat gctggtatat taggcgggct 4980
attatcatac gtagccctcg gatttatagt tgattggacg ttaagactta ttgtcattaa 5040
cttgactctt gctggcaaga gaacatacca gggtacggtc gaacatgtaa tgggtaaaaa 5100
agggaaattg ctgattctat ttacaaacgg gttatttgca tttggtggat gtattgg 5157
<210> 87
<211> 5600
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、乳酸克鲁维酵母和构巢曲霉
<400> 87
gaaggtgcta ttgttggtat tagaggtggt gttattggtg tcggtactga cattggtggt 60
tccattagag tcccagctgc tttcaacttt ttatacggtt tgagaccatc tcacggtaga 120
ttgccatatg ctaaaatggc taactctatg gaaggtcaag aaaccgttca ctccgtcgtt 180
ggtcctatca ctcactccgt cgaagacttg agattgttca ccaaatctgt cttgggtcaa 240
gaaccttgga agtacgactc taaggtcatc ccaatgccat ggagacaatc tgaatctgac 300
atcattgcct ctaagattaa gaatggtggt ttgaacattg gttattacaa tttcgacggt 360
aacgtcttgc cacacccacc aattttacgt ggtgtcgaaa ctaccgttgc cgctttggcc 420
aaggctggtc acaccgttac tccatggact ccatacaagc atgatttcgg tcatgacttg 480
atttcccaca tctatgctgc tgatggttct gccgacgtca tgagagacat ttctgcctct 540
ggtgagccag ccatccctaa cattaaggac ttgttgaacc caaatattaa ggctgttaac 600
atgaacgaat tgtgggacac tcatttacaa aagtggaact atcaaatgga atacttggaa 660
aagtggcgtg aagctgaaga aaaagctggt aaggaattgg acgctattat cgctccaatt 720
actcctaccg ccgctgtcag acacgatcaa ttcagatact acggttacgc ctccgttatt 780
aacttattgg atttcacctc tgttgtcgtc ccagtcactt tcgctgataa gaatattgat 840
aagaagaacg aatcttttaa agctgtttcc gaattggatg ctttggttca agaagaatac 900
gacccagagg cttatcacgg tgctcctgtt gctgttcaag ttattggtag aagattgtcc 960
gaagagagaa ctttggctat cgccgaagaa gtcggtaaat tgttgggtaa cgtcgtcact 1020
ccataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata 1080
agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt 1140
aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac 1200
cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg 1260
tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga 1320
ggacaacaca taacttcgta taatgtatgc tatacgaagt tatctcgagg aagcccgaaa 1380
gagttatcgt tactccgatt attttgtaca gctgatggga ccttgccgtc ttcatttttt 1440
tttttttcac ctatagagcc gggcagagct gcccggctca actaagggcc ggaaaaaaaa 1500
cggaaaaaag aaagccaagc gtgtagacgt agtataacag tatatctgac acgcacgtga 1560
tgaccacgta atcgcatcgc ccctcacatc tcacctctca ccgctgactc agcttcacta 1620
aaaaggaaaa tatatactct ttcccaggca aggtgacagc ggtccccgtc tcctccacaa 1680
aggcctctcc tggggtttga gcaagtctaa gtttacgtag cataaaaatt ctcggattgc 1740
gtcaaataat aaaaaaagta actccacttc tacttctaca tcggaaaaac attccattca 1800
catatcgtct ttggcctatc ttgttttgtc cttggtagat caggtcagta caaacgcaac 1860
acgaaagaac aaaaaaagaa gaaaaacaga aggccaagac agggtcaatg agactgttgt 1920
cctcctactg tccctatgtc tctggccgat cacgcgccat tgtccctcag aaacaaatca 1980
aacacccaca ccccgggcac ccaaagtccc cacccacacc accaatacgt aaacggggcg 2040
ccccctgcag gccctcctgc gcgcggcctc ccgccttgct tctctcccct cccttttctt 2100
tttccagttt tccctatttt gtcccttttt ccgcacaaca agtatcagaa tgggttcatc 2160
aaatctatcc aacctaattc gcacgtagac tggcttggta ttggcagttt cgcagttata 2220
tatatactac catgagtgaa actgttacgt taccttaaat tctttctccc tttaattttc 2280
ttttatctta ctctcctaca taagacatca agaaacaatt gtatattgta cacccccccc 2340
cctccacaaa cacaaatatt gataatataa agtctagaat gaataacaac aacattacac 2400
cagagtcaga ctcaatgaag agtaacgata acgatcaaac caatgattac atgccagatg 2460
tggctgattt cgatcataca caaacaaaca caaacgaaat tgctagagca atttctcacc 2520
caggttctgt tctgtcaaga gttgcaagct acgtcagcag aaaggacaga tacgtagacg 2580
agaatggcaa tgaagtttgg caagacgatg aagtgagtat cctaatggag gaagatgaga 2640
ctcctgattt cacatggaaa aacattagac attacgctat cactagattc actacattga 2700
cagaacttca cagagtgtct atggaaaaca tcaacccaat tccagaattg agaaaaatga 2760
cattacataa ttggaactac ttctttatgg ggtatgccgc gtggttgtgt gctgcttggg 2820
catttttcgc cgtttccgtt agtacagccc ctcttgcaac cctttatggc aaggaaacaa 2880
aagacatttc atggggtcta tccttagtct tattcgtcag atctgctgga gcgattatct 2940
ttggcatctg gaccgacaat tactccagaa agtggccata cattacatgc ttaggtcttt 3000
tcttgatctg tcagctttgc acaccttggg caaagaccta cactcaattc ttgggagttc 3060
gttggatttc cggtatcgca atgggtggaa tttacgcctg tgcttctgca acagcgattg 3120
aggatgcacc agtaaaggct cgttctttct tatccggctt attcttcact gcctacgcca 3180
tgggttttat cttcgcaata atcttttaca gagcattctt aaacgtcaac ggtgaaaact 3240
actggaaggt tcaattctgg ttttcaatat ggttaccagc tgtactgata ttgtggagac 3300
tcgtatggcc agaaactaag tacttcacca aagtactaaa agctagacaa ctaatgcgtg 3360
atgatgcaat agccaaaaac ggtggtcaac cactcccaaa gttgtctttc aagcaaaagt 3420
tcgccaatgt gaaaaagacc gttagtaaat actggctttt gtttggttac ttaattctct 3480
tgttggttgg tcctaactac ctaacacacg cctctcaaga cttgtttcct acaatgttaa 3540
gagcacaatt gagattttct gaagatgctg tcaccgtggc cattgtagtt gtttgtctgg 3600
gtagcattgc tggggggatg tttttcggac agttgatgga gatcactggt agaagagttg 3660
gcctactatt ggccctaatc atggctggct gctttactta ccctgccttc atgcttaaaa 3720
cttcatctgc agtgctgggg gcaggcttta tgctctggtt ctcaatctta ggtgtgtggg 3780
gtgttttacc aatccattta tcagaattgt cccctccaga agctagagca ttagtctctg 3840
gattagcata tcagctgggt aatctagctt cagcagcttc tgttgttata gagaatgatt 3900
tggcggattt gtatccaata gaatggaatt ctgctggaga agttacaaac aaggactact 3960
ctaaggtaat ggctattctt acaggttctt cagtcatttt cactttcgtg ttggttttcg 4020
ttggtcacga aaagtttcat agagatttgt catccccaca tcttaagtca tacatcgaga 4080
gagtcgatca aacagaagag gtcgcagcta tgactggatc tactgcgaac tctatctcta 4140
gtaagccttc agatgatcag ctcgaaaaag tttcagtcta gttaattaat ttaccagctt 4200
actatccttc ttgaaaatat gcactctata tcttttagtt cttaattgca acacatagat 4260
ttgctgtata acgaatttta tgctattttt ttaatttgga gttcggtgat gaaagtgtca 4320
cagcgaattt cctcacatgt agggaccgaa ttgtttacaa gttctctgta ccaccatgga 4380
gacatcaaag attgaaaatc tatggaaaga tatggacggt agcaacaaga atatagcacg 4440
agccgcggag ttcatttcgt tacttttgat atcgctcaca actattgcga agcgcttcag 4500
tgaaaaaatc ataaggaaaa gttgtaaata ttattggtag tattcgtttg gtaaagtaga 4560
gggggtaatt tttccccttt attttgttca tacattctta aattgctttg cctctccttt 4620
tggaaagcta tacttcggag cactgttgag cgaaggctca ggccggcata tgacgtttta 4680
ttacctttga tcacatttcc acgccatttc gcattctcac cctcataagt catacaccga 4740
aaagaaagtt taagggatca atgagcttac tataatctca gtatatttat ttttatcgat 4800
gattcaccac aacaatcttg ctcccgaaaa gaaagcagac ggagtagaag catttgaaac 4860
tccttcagac cttcaagtat atatatatat atatatatat gtatatgtgt acattttcac 4920
gctaatacta atgtataatt agaagataat ttttactcat ttttcgttat cttcacgtca 4980
cccgaaccta gaaccaaatg tcattttcac gatatgtaaa tagtgaaata ggcaaaaacg 5040
ccaaaaagta gtaagcgcaa catacactaa accattaaag aatatctcga ccagaatcta 5100
acagatatac atgttccgat aatgtctgag ttaggtgagt attctaaatt agaaaacaaa 5160
gagcttagaa cggagtttga attgacaaat tttccttttc caggcacaac tgataacgac 5220
tccgatgacg gaagccaagg gcagaactct ttgaatatca ttactcctga catggatgat 5280
actctggtta atgatgtact tcgagaaaac gataaaaagt ctagtatgag aatggctttt 5340
atgaatctag caaactctat tcttggtgcc ggaataatta ctcagccgtt cgcgatcaaa 5400
aatgctggta tattaggcgg gctattatca tacgtagccc tcggatttat agttgattgg 5460
acgttaagac ttattgtcat taacttgact cttgctggca agagaacata ccagggtacg 5520
gtcgaacatg taatgggtaa aaaagggaaa ttgctgattc tatttacaaa cgggttattt 5580
gcatttggtg gatgtattgg 5600
<210> 88
<211> 5233
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、乳酸克鲁维酵母和构巢曲霉
<400> 88
gaaggtgcta ttgttggtat tagaggtggt gttattggtg tcggtactga cattggtggt 60
tccattagag tcccagctgc tttcaacttt ttatacggtt tgagaccatc tcacggtaga 120
ttgccatatg ctaaaatggc taactctatg gaaggtcaag aaaccgttca ctccgtcgtt 180
ggtcctatca ctcactccgt cgaagacttg agattgttca ccaaatctgt cttgggtcaa 240
gaaccttgga agtacgactc taaggtcatc ccaatgccat ggagacaatc tgaatctgac 300
atcattgcct ctaagattaa gaatggtggt ttgaacattg gttattacaa tttcgacggt 360
aacgtcttgc cacacccacc aattttacgt ggtgtcgaaa ctaccgttgc cgctttggcc 420
aaggctggtc acaccgttac tccatggact ccatacaagc atgatttcgg tcatgacttg 480
atttcccaca tctatgctgc tgatggttct gccgacgtca tgagagacat ttctgcctct 540
ggtgagccag ccatccctaa cattaaggac ttgttgaacc caaatattaa ggctgttaac 600
atgaacgaat tgtgggacac tcatttacaa aagtggaact atcaaatgga atacttggaa 660
aagtggcgtg aagctgaaga aaaagctggt aaggaattgg acgctattat cgctccaatt 720
actcctaccg ccgctgtcag acacgatcaa ttcagatact acggttacgc ctccgttatt 780
aacttattgg atttcacctc tgttgtcgtc ccagtcactt tcgctgataa gaatattgat 840
aagaagaacg aatcttttaa agctgtttcc gaattggatg ctttggttca agaagaatac 900
gacccagagg cttatcacgg tgctcctgtt gctgttcaag ttattggtag aagattgtcc 960
gaagagagaa ctttggctat cgccgaagaa gtcggtaaat tgttgggtaa cgtcgtcact 1020
ccataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata 1080
agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt 1140
aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac 1200
cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg 1260
tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga 1320
ggacaacaca taacttcgta taatgtatgc tatacgaagt tatctcgagg aagagactaa 1380
tcaaagaatc gttttctcaa aaaatttaat atcttaactg atagtttgat caaaggggca 1440
aaacgtaggg gcaaacaaac ggaaaaatcg tttctcaaat tttctgatgc caagaactct 1500
aaccagtctt atctaaaaat tgccttatga tccgtctctc cggttacagc ctgtgtaact 1560
gattaatcct gcctttctaa tcaccattct aatgttttaa ttaagggatt ttgtcttcat 1620
taacggcttt cgctcataaa aatgttatga cgttttgccc gcaggcggga aaccatccac 1680
ttcacgagac tgatctcctc tgccggaaca ccgggcatct ccaacttata agttggagaa 1740
ataagagaat ttcagattga gagaatgaaa aaaaaaaaaa aaaaggcaga ggagagcata 1800
aaaatggggt tcactttttg gtaaagctat agcatgccta tcacatataa atagagtgcc 1860
agtagcgact tttttcacac tcgaaatact cttactactg ctctcttgtt gtttttatca 1920
cttcttgttt cttcttggta aatagaatat caagctacaa aaagcataca atcaactatc 1980
aactattaac tatatcgtaa tacactctag aatgaataac aacaacatta caccagagtc 2040
agactcaatg aagagtaacg ataacgatca aaccaatgat tacatgccag atgtggctga 2100
tttcgatcat acacaaacaa acacaaacga aattgctaga gcaatttctc acccaggttc 2160
tgttctgtca agagttgcaa gctacgtcag cagaaaggac agatacgtag acgagaatgg 2220
caatgaagtt tggcaagacg atgaagtgag tatcctaatg gaggaagatg agactcctga 2280
tttcacatgg aaaaacatta gacattacgc tatcactaga ttcactacat tgacagaact 2340
tcacagagtg tctatggaaa acatcaaccc aattccagaa ttgagaaaaa tgacattaca 2400
taattggaac tacttcttta tggggtatgc cgcgtggttg tgtgctgctt gggcattttt 2460
cgccgtttcc gttagtacag cccctcttgc aaccctttat ggcaaggaaa caaaagacat 2520
ttcatggggt ctatccttag tcttattcgt cagatctgct ggagcgatta tctttggcat 2580
ctggaccgac aattactcca gaaagtggcc atacattaca tgcttaggtc ttttcttgat 2640
ctgtcagctt tgcacacctt gggcaaagac ctacactcaa ttcttgggag ttcgttggat 2700
ttccggtatc gcaatgggtg gaatttacgc ctgtgcttct gcaacagcga ttgaggatgc 2760
accagtaaag gctcgttctt tcttatccgg cttattcttc actgcctacg ccatgggttt 2820
tatcttcgca ataatctttt acagagcatt cttaaacgtc aacggtgaaa actactggaa 2880
ggttcaattc tggttttcaa tatggttacc agctgtactg atattgtgga gactcgtatg 2940
gccagaaact aagtacttca ccaaagtact aaaagctaga caactaatgc gtgatgatgc 3000
aatagccaaa aacggtggtc aaccactccc aaagttgtct ttcaagcaaa agttcgccaa 3060
tgtgaaaaag accgttagta aatactggct tttgtttggt tacttaattc tcttgttggt 3120
tggtcctaac tacctaacac acgcctctca agacttgttt cctacaatgt taagagcaca 3180
attgagattt tctgaagatg ctgtcaccgt ggccattgta gttgtttgtc tgggtagcat 3240
tgctgggggg atgtttttcg gacagttgat ggagatcact ggtagaagag ttggcctact 3300
attggcccta atcatggctg gctgctttac ttaccctgcc ttcatgctta aaacttcatc 3360
tgcagtgctg ggggcaggct ttatgctctg gttctcaatc ttaggtgtgt ggggtgtttt 3420
accaatccat ttatcagaat tgtcccctcc agaagctaga gcattagtct ctggattagc 3480
atatcagctg ggtaatctag cttcagcagc ttctgttgtt atagagaatg atttggcgga 3540
tttgtatcca atagaatgga attctgctgg agaagttaca aacaaggact actctaaggt 3600
aatggctatt cttacaggtt cttcagtcat tttcactttc gtgttggttt tcgttggtca 3660
cgaaaagttt catagagatt tgtcatcccc acatcttaag tcatacatcg agagagtcga 3720
tcaaacagaa gaggtcgcag ctatgactgg atctactgcg aactctatct ctagtaagcc 3780
ttcagatgat cagctcgaaa aagtttcagt ctagttaatt aatttaccag cttactatcc 3840
ttcttgaaaa tatgcactct atatctttta gttcttaatt gcaacacata gatttgctgt 3900
ataacgaatt ttatgctatt tttttaattt ggagttcggt gatgaaagtg tcacagcgaa 3960
tttcctcaca tgtagggacc gaattgttta caagttctct gtaccaccat ggagacatca 4020
aagattgaaa atctatggaa agatatggac ggtagcaaca agaatatagc acgagccgcg 4080
gagttcattt cgttactttt gatatcgctc acaactattg cgaagcgctt cagtgaaaaa 4140
atcataagga aaagttgtaa atattattgg tagtattcgt ttggtaaagt agagggggta 4200
atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag 4260
ctatacttcg gagcactgtt gagcgaaggc tcaggccggc atatgacgtt ttattacctt 4320
tgatcacatt tccacgccat ttcgcattct caccctcata agtcatacac cgaaaagaaa 4380
gtttaaggga tcaatgagct tactataatc tcagtatatt tatttttatc gatgattcac 4440
cacaacaatc ttgctcccga aaagaaagca gacggagtag aagcatttga aactccttca 4500
gaccttcaag tatatatata tatatatata tatgtatatg tgtacatttt cacgctaata 4560
ctaatgtata attagaagat aatttttact catttttcgt tatcttcacg tcacccgaac 4620
ctagaaccaa atgtcatttt cacgatatgt aaatagtgaa ataggcaaaa acgccaaaaa 4680
gtagtaagcg caacatacac taaaccatta aagaatatct cgaccagaat ctaacagata 4740
tacatgttcc gataatgtct gagttaggtg agtattctaa attagaaaac aaagagctta 4800
gaacggagtt tgaattgaca aattttcctt ttccaggcac aactgataac gactccgatg 4860
acggaagcca agggcagaac tctttgaata tcattactcc tgacatggat gatactctgg 4920
ttaatgatgt acttcgagaa aacgataaaa agtctagtat gagaatggct tttatgaatc 4980
tagcaaactc tattcttggt gccggaataa ttactcagcc gttcgcgatc aaaaatgctg 5040
gtatattagg cgggctatta tcatacgtag ccctcggatt tatagttgat tggacgttaa 5100
gacttattgt cattaacttg actcttgctg gcaagagaac ataccagggt acggtcgaac 5160
atgtaatggg taaaaaaggg aaattgctga ttctatttac aaacgggtta tttgcatttg 5220
gtggatgtat tgg 5233
<210> 89
<211> 5565
<212> DNA
<213> 人工序列(Artificial Sequence)
<220>
<223> 由以下多种生物体组合在一起的序列：酿酒酵母、乳酸克鲁维酵母和构巢曲霉
<400> 89
gaaggtgcta ttgttggtat tagaggtggt gttattggtg tcggtactga cattggtggt 60
tccattagag tcccagctgc tttcaacttt ttatacggtt tgagaccatc tcacggtaga 120
ttgccatatg ctaaaatggc taactctatg gaaggtcaag aaaccgttca ctccgtcgtt 180
ggtcctatca ctcactccgt cgaagacttg agattgttca ccaaatctgt cttgggtcaa 240
gaaccttgga agtacgactc taaggtcatc ccaatgccat ggagacaatc tgaatctgac 300
atcattgcct ctaagattaa gaatggtggt ttgaacattg gttattacaa tttcgacggt 360
aacgtcttgc cacacccacc aattttacgt ggtgtcgaaa ctaccgttgc cgctttggcc 420
aaggctggtc acaccgttac tccatggact ccatacaagc atgatttcgg tcatgacttg 480
atttcccaca tctatgctgc tgatggttct gccgacgtca tgagagacat ttctgcctct 540
ggtgagccag ccatccctaa cattaaggac ttgttgaacc caaatattaa ggctgttaac 600
atgaacgaat tgtgggacac tcatttacaa aagtggaact atcaaatgga atacttggaa 660
aagtggcgtg aagctgaaga aaaagctggt aaggaattgg acgctattat cgctccaatt 720
actcctaccg ccgctgtcag acacgatcaa ttcagatact acggttacgc ctccgttatt 780
aacttattgg atttcacctc tgttgtcgtc ccagtcactt tcgctgataa gaatattgat 840
aagaagaacg aatcttttaa agctgtttcc gaattggatg ctttggttca agaagaatac 900
gacccagagg cttatcacgg tgctcctgtt gctgttcaag ttattggtag aagattgtcc 960
gaagagagaa ctttggctat cgccgaagaa gtcggtaaat tgttgggtaa cgtcgtcact 1020
ccataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata 1080
agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt 1140
aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac 1200
cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg 1260
tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga 1320
ggacaacaca taacttcgta taatgtatgc tatacgaagt tatctcgagg acaaccagga 1380
cgtaaagggt agcctcccca taacataaac tcaataaaat atatagtctt caacttgaaa 1440
aaggaacaag ctcatgcaaa gaggtggtac ccgcacgccg aaatgcatgc aagtaaccta 1500
ttcaaagtaa tatctcatac atgtttcatg agggtaacaa catgcgactg ggtgagcata 1560
tgttccgctg atgtgatgtg caagataaac aagcaagaca gaaactaact tcttcttcat 1620
gtaataaaca caccccgcgt ttatttacct atctttaaac ttcaacacct tatatcataa 1680
ctaatatttc ttgagataag cacactgcac ccataccttc cttaaaaacg tagcttccag 1740
tttttggtgg ttctggcttc cttcccgatt ccgcccgcta aacgcataat tttgttgcct 1800
ggtggcattt gcaaaatgca taacctatgc atttaaaaga ttatgtatgc tcttctgact 1860
tttcgtgtga tgaggctcgt ggaaaaaatg aataatttat gaatttgaga acaattttgt 1920
gttgttacgg tattttacta tggaataatc aatcaattga ggattttatg caaatatcgt 1980
ttgaatattt ttccgaccct ttgagtactt ttcttcataa ttgcataata ttgtccgctg 2040
cccgtttttc tgttagacgg tgtcttgatc tacttgctat cgttcaacac caccttattt 2100
tctaactatt ttttttttag ctcatttgaa tcagcttatg gtgatggcac atttttgcat 2160
aaacctagct gtcctcgttg aacataggaa aaaaaaatat ataaacaagg ctctttcact 2220
ctccttggaa tcagatttgg gtttgttccc tttattttca tatttcttgt catattcttt 2280
tctcaattat tatcttctac tcataacctc acgcaaaata acacagtcaa atcaatctct 2340
agaatgaata acaacaacat tacaccagag tcagactcaa tgaagagtaa cgataacgat 2400
caaaccaatg attacatgcc agatgtggct gatttcgatc atacacaaac aaacacaaac 2460
gaaattgcta gagcaatttc tcacccaggt tctgttctgt caagagttgc aagctacgtc 2520
agcagaaagg acagatacgt agacgagaat ggcaatgaag tttggcaaga cgatgaagtg 2580
agtatcctaa tggaggaaga tgagactcct gatttcacat ggaaaaacat tagacattac 2640
gctatcacta gattcactac attgacagaa cttcacagag tgtctatgga aaacatcaac 2700
ccaattccag aattgagaaa aatgacatta cataattgga actacttctt tatggggtat 2760
gccgcgtggt tgtgtgctgc ttgggcattt ttcgccgttt ccgttagtac agcccctctt 2820
gcaacccttt atggcaagga aacaaaagac atttcatggg gtctatcctt agtcttattc 2880
gtcagatctg ctggagcgat tatctttggc atctggaccg acaattactc cagaaagtgg 2940
ccatacatta catgcttagg tcttttcttg atctgtcagc tttgcacacc ttgggcaaag 3000
acctacactc aattcttggg agttcgttgg atttccggta tcgcaatggg tggaatttac 3060
gcctgtgctt ctgcaacagc gattgaggat gcaccagtaa aggctcgttc tttcttatcc 3120
ggcttattct tcactgccta cgccatgggt tttatcttcg caataatctt ttacagagca 3180
ttcttaaacg tcaacggtga aaactactgg aaggttcaat tctggttttc aatatggtta 3240
ccagctgtac tgatattgtg gagactcgta tggccagaaa ctaagtactt caccaaagta 3300
ctaaaagcta gacaactaat gcgtgatgat gcaatagcca aaaacggtgg tcaaccactc 3360
ccaaagttgt ctttcaagca aaagttcgcc aatgtgaaaa agaccgttag taaatactgg 3420
cttttgtttg gttacttaat tctcttgttg gttggtccta actacctaac acacgcctct 3480
caagacttgt ttcctacaat gttaagagca caattgagat tttctgaaga tgctgtcacc 3540
gtggccattg tagttgtttg tctgggtagc attgctgggg ggatgttttt cggacagttg 3600
atggagatca ctggtagaag agttggccta ctattggccc taatcatggc tggctgcttt 3660
acttaccctg ccttcatgct taaaacttca tctgcagtgc tgggggcagg ctttatgctc 3720
tggttctcaa tcttaggtgt gtggggtgtt ttaccaatcc atttatcaga attgtcccct 3780
ccagaagcta gagcattagt ctctggatta gcatatcagc tgggtaatct agcttcagca 3840
gcttctgttg ttatagagaa tgatttggcg gatttgtatc caatagaatg gaattctgct 3900
ggagaagtta caaacaagga ctactctaag gtaatggcta ttcttacagg ttcttcagtc 3960
attttcactt tcgtgttggt tttcgttggt cacgaaaagt ttcatagaga tttgtcatcc 4020
ccacatctta agtcatacat cgagagagtc gatcaaacag aagaggtcgc agctatgact 4080
ggatctactg cgaactctat ctctagtaag ccttcagatg atcagctcga aaaagtttca 4140
gtctagttaa ttaatttacc agcttactat ccttcttgaa aatatgcact ctatatcttt 4200
tagttcttaa ttgcaacaca tagatttgct gtataacgaa ttttatgcta tttttttaat 4260
ttggagttcg gtgatgaaag tgtcacagcg aatttcctca catgtaggga ccgaattgtt 4320
tacaagttct ctgtaccacc atggagacat caaagattga aaatctatgg aaagatatgg 4380
acggtagcaa caagaatata gcacgagccg cggagttcat ttcgttactt ttgatatcgc 4440
tcacaactat tgcgaagcgc ttcagtgaaa aaatcataag gaaaagttgt aaatattatt 4500
ggtagtattc gtttggtaaa gtagaggggg taatttttcc cctttatttt gttcatacat 4560
tcttaaattg ctttgcctct ccttttggaa agctatactt cggagcactg ttgagcgaag 4620
gctcaggccg gcatatgacg ttttattacc tttgatcaca tttccacgcc atttcgcatt 4680
ctcaccctca taagtcatac accgaaaaga aagtttaagg gatcaatgag cttactataa 4740
tctcagtata tttattttta tcgatgattc accacaacaa tcttgctccc gaaaagaaag 4800
cagacggagt agaagcattt gaaactcctt cagaccttca agtatatata tatatatata 4860
tatatgtata tgtgtacatt ttcacgctaa tactaatgta taattagaag ataattttta 4920
ctcatttttc gttatcttca cgtcacccga acctagaacc aaatgtcatt ttcacgatat 4980
gtaaatagtg aaataggcaa aaacgccaaa aagtagtaag cgcaacatac actaaaccat 5040
taaagaatat ctcgaccaga atctaacaga tatacatgtt ccgataatgt ctgagttagg 5100
tgagtattct aaattagaaa acaaagagct tagaacggag tttgaattga caaattttcc 5160
ttttccaggc acaactgata acgactccga tgacggaagc caagggcaga actctttgaa 5220
tatcattact cctgacatgg atgatactct ggttaatgat gtacttcgag aaaacgataa 5280
aaagtctagt atgagaatgg cttttatgaa tctagcaaac tctattcttg gtgccggaat 5340
aattactcag ccgttcgcga tcaaaaatgc tggtatatta ggcgggctat tatcatacgt 5400
agccctcgga tttatagttg attggacgtt aagacttatt gtcattaact tgactcttgc 5460
tggcaagaga acataccagg gtacggtcga acatgtaatg ggtaaaaaag ggaaattgct 5520
gattctattt acaaacgggt tatttgcatt tggtggatgt attgg 5565

Claims (20)

1.一种经遗传修饰的酵母，其包含编码一元羧酸/单羧酸转运蛋白的异源基因和一种或多种编码乳酸脱氢酶(细胞色素)的异源基因(分类为EC 1.1.2.3或1.1.2.4)，
其中当所述酵母存在于包含乳酸和己糖的发酵培养基中时，所述酵母能够消耗乳酸并产生乙醇。
2.如权利要求1所述的酵母，其中当在48h取样时间所述发酵培养基中己糖的浓度为5g/L或更高时，如根据乳酸消耗评估方案所测量的，所述酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的L-乳酸消耗速率。
3.如权利要求1所述的酵母，其中当在48h取样时间所述发酵培养基中己糖的浓度为5g/L或更高时，如根据乳酸消耗评估方案所测量的，所述酵母具有至少0.015、0.020、0.025、0.030、0.035或0.040gL^h-1的D-乳酸消耗速率。
4.如权利要求1-3中任一项所述的酵母，其中所述酵母的D-乳酸、L-乳酸和/或总乳酸消耗速率大于不含编码一元羧酸/单羧酸转运蛋白的异源基因的酵母。
5.如权利要求1-4中任一项所述的酵母，其中当所述酵母存在于包含乳酸和己糖的发酵培养基中时，所述酵母能够消耗D-乳酸和L-乳酸两者。
6.如权利要求1-5中任一项所述的酵母，其中所述一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的D-乳酸脱氢酶(DLD)基因。
7.如权利要求1-6中任一项所述的酵母，其中所述一种或多种异源乳酸脱氢酶(细胞色素)基因包含过表达的细胞色素b2(CYB2)基因。
8.如权利要求1-7中任一项所述的酵母，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:40、SEQ ID NO:42、SEQ ID NO:64、SEQ IDNO:66、SEQ ID NO:68、SEQ ID NO:70、SEQ ID NO:72或SEQ ID NO:74。
9.如权利要求1-8中任一项所述的酵母，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:15、SEQ ID NO:17、SEQ ID NO:54、SEQ IDNO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62。
10.一种经遗传修饰的酵母，其包含编码单羧酸/质子同向转运蛋白的异源基因和编码葡糖淀粉酶的异源基因，
其中当所述酵母存在于包含乳酸和己糖、淀粉或己糖与淀粉的组合的发酵培养基中时，所述酵母能够消耗乳酸并产生乙醇。
11.如权利要求1-10中任一项所述的酵母，其中所述酵母编码葡糖淀粉酶多肽，所述葡糖淀粉酶多肽与来自扣囊复膜酵母或米根霉的野生型多肽具有至少80％的序列同一性。
12.如权利要求1-11中任一项所述的酵母，其中所述酵母从酿酒酵母物种的宿主酵母进行遗传修饰。
13.一种经遗传修饰的酵母，其包含酿酒酵母物种的酵母，其中所述酵母包含编码单羧酸/质子同向转运蛋白的异源基因。
14.如权利要求1-13中任一项所述的酵母，其能够以大于80g/L、90g/L、100g/L、110g/L、120g/L、125g/L 130g/L、135g/L、140g/L、145g/L、150g/L、155g/L、160g/L或165g/L的滴度产生乙醇。
15.如权利要求1-15中任一项所述的酵母，其中所述编码一元羧酸/单羧酸转运蛋白的异源基因来自乳酸克鲁维酵母。
16.如权利要求1-15中任一项所述的酵母，其中所述酵母编码多肽，所述多肽具有与以下氨基酸序列中的任一者具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:24、SEQ ID NO:26、SEQ ID NO:28、SEQID NO:76或SEQ ID NO:78。
17.如权利要求1-16中任一项所述的酵母，其包含在SEQ ID NO:15、SEQ ID NO:17、SEQID NO:54、SEQ ID NO:56、SEQ ID NO:58、SEQ ID NO:60或SEQ ID NO:62中的指定位置处的以下残基中的一者或多者：Lys349、Tyrl43、Tyr254和His373。
18.一种用于生产乙醇的方法，所述方法包括用如权利要求1-17中任一项所述的酵母使底物发酵。
19.如权利要求18所述的方法，其具有至少0.5、1、2、3或4mmol02/(L-h)的体积氧摄取速率(OUR)。
20.如权利要求18-19中任一项所述的方法，其中发酵结束时的乙醇滴度为至少80、90、100、110、120、130、135、140、145、150、155或160g/L。
CN201780066177.3A 2016-09-16 2017-09-15 经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺 Pending CN109906270A (zh)

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