CN110582567B

CN110582567B - 经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法

Info

Publication number: CN110582567B
Application number: CN201880029455.2A
Authority: CN
Inventors: 彼得·艾伦·豪尔特; 格雷戈里·迈克尔·庞特; 布来恩·J·拉什
Original assignee: Cargill Inc
Current assignee: Cargill Inc
Priority date: 2017-05-04
Filing date: 2018-05-04
Publication date: 2024-03-08
Anticipated expiration: 2038-05-04
Also published as: US20210301275A1; PL3619300T3; ES2972654T3; US11802266B2; WO2018204798A1; US11041218B2; EP3619300A4; EP3619300B1; CN110582567A; US20200063221A1; EP3619300A1; CA3062324A1; BR112019023085A2

Abstract

本发明涉及具有异源海藻糖酶基因的遗传工程改造的酵母，以及使用此类酵母的发酵方法。所述酵母能够以足以将大量海藻糖转化为葡萄糖的量表达海藻糖酶，从而提高发酵中产物的收率，和/或减少或消除对向所述发酵中添加外源海藻糖酶的需要。所述酵母还可包含其它异源基因，用于表达可用于提高收率和/或减少或消除对向所述发酵中添加外源酶的需要的酶。

Description

经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法

相关申请的交叉引用

本申请要求于2017年5月4日提交的美国临时专利申请号62/501,288、2018年2月28日提交的美国临时专利申请号62/636,716和2018年3月27日提交的美国临时专利申请号62/648,679的权益，所有所述美国临时专利申请由此通过引用整体并入。

序列表

2018年5月4日创建的大小为761千字节的标题为“N00525_ST25.txt”的ASCII文本文件的全部内容由此通过引用并入本文。

发明背景

海藻糖是在包括酿酒酵母在内的微生物中产生的二糖。海藻糖通常是由于生物体受到应激而产生的。海藻糖酶是催化海藻糖向葡萄糖转化的糖苷水解酶。一些微生物可以天然产生中性pH海藻糖酶和/或酸性海藻糖酶。然而，野生型酵母不会产生大量的海藻糖酶。

发明内容

本发明涉及可以表达异源海藻糖酶的遗传工程改造的酵母。由酵母表达的海藻糖酶通过将由酵母产生的海藻糖或以其它方式存在于发酵液中的海藻糖转化为葡萄糖而增加了来自发酵的乙醇产量。

一方面，本公开涉及包含编码海藻糖酶(EC 3.2.1.28)的异源基因的经遗传修饰的酵母，其中当酵母存在于包含海藻糖的发酵培养基中时，该酵母能够产生乙醇。在一些实施方案中，海藻糖酶是酸性海藻糖酶。在一些实施方案中，海藻糖酶是中性海藻糖酶。在一些实施方案中，酵母编码酸性海藻糖酶和中性海藻糖酶。在一些实施方案中，编码海藻糖酶的基因来自乳酸克鲁维酵母(Kluyveromyces lactis)。在一些实施方案中，编码海藻糖酶的基因来自近平滑假丝酵母(Candida parapsilosis)。在一些实施方案中，编码海藻糖酶的基因来自光滑假丝酵母(Candida glabrata)。在一些实施方案中，编码海藻糖酶的基因来自稻瘟病菌(Magnaporthe grisea)。在一些实施方案中，由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQ ID NO：1、SEQ ID NO：2、SEQ ID NO：3或SEQ ID NO：87。在一些实施方案中，由酵母编码的海藻糖酶多肽包括与SEQ ID NO：83具有至少70％、80％、90％或95％的序列同一性的序列。在一些实施方案中，由酵母编码的海藻糖酶多肽包括与SEQ ID NO：84和/或SEQ ID NO：85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列。在一些实施方案中，酵母是经遗传修饰的酿酒酵母。在一些实施方案中，由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQ ID NO：88、SEQ ID NO：89、SEQ ID NO：90或SEQ ID NO：91。

在一些实施方案中，酵母包含对于海藻糖酶所源自的物种是非天然的异源海藻糖酶的信号序列。在一些实施方案中，异源海藻糖酶包含非天然信号序列。在一些实施方案中，异源海藻糖酶包含其天然信号序列。在一些实施方案中，信号序列是MFα2信号序列。在一些实施方案中，MFα2信号序列是SEQ ID NO：4。在一些实施方案中，MFα2信号序列与SEQID NO：4具有至少84％、89％或94％的序列同一性。在一些实施方案中，由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQ ID NO：8、SEQ ID NO：11、SEQ ID NO：14或SEQ ID NO：92。

一方面，酵母包含至少一种编码除海藻糖酶外的多肽的异源基因。在一些实施方案中，酵母包含编码葡糖淀粉酶(EC 3.2.1.3)的异源基因。在一些实施方案中，编码葡糖淀粉酶的异源基因是来自选自由以下组成的组的物种的葡糖淀粉酶基因：树脂枝胞霉(Cladosporium resinae)、黑曲霉(Aspergillus niger)、泡盛曲霉(Aspergillusawamori)、米曲霉(Aspergillus oryzae)、白曲霉(Aspergillus kawachii)、白宇佐美曲霉(Aspergillus shirousami)、食腺嘌呤芽生葡萄孢酵母(Blastobotrys adeninivorans)、白假丝酵母(Candida albicans)、米根霉(Rhizopus oryzae)、粟酒裂殖酵母(Schizosaccharomyces pombe)、扣囊复膜酵母菌(Saccharomycopsis fibuligera)、布鲁塞尔酒香酵母(Brettanomyces bruxellensis)和产朊假丝酵母(Cyberlindnerajadinii)。在一些实施方案中，由酵母编码的葡糖淀粉酶与以下多肽序列中的至少一个具有至少70％、80％、90％或95％的序列同一性：SEQ ID NO：16、SEQ ID NO：17、SEQ ID NO：18或SEQ ID NO：19。在一些实施方案中，酵母包含编码异麦芽糖酶(EC 3.2.1.10)的异源基因。在一些实施方案中，酵母包含与SEQ ID NO：20的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白的异源基因。在一些实施方案中，酵母包含与SEQ ID NO：21的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白的异源基因。

一方面，酵母包括与乳酸消耗有关的特征。在一些实施方案中，酵母包含编码细胞色素b2(CYB2)(EC 1.1.2.3)多肽的异源基因。在一些实施方案中，CYB2多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：SEQ ID NO:27、SEQ ID NO:28、SEQ IDNO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32或SEQ ID NO:33。在一些实施方案中，酵母编码CYB2多肽，所述CYB2多肽在SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ IDNO:30、SEQ ID NO:31、SEQ ID NO:32或SEQ ID NO:33中的指示位置处包含一个或多个以下残基：Lys349、Tyr143、Tyr254和His373。在一些实施方案中，酵母包含编码D乳酸脱氢酶(DLD)(EC 1.1.2.4)多肽的异源基因。在一些实施方案中，DLD多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40或SEQ ID NO:41。在一些实施方案中，酵母包含编码单羧酸/单羧酸盐转运蛋白的异源基因。在一些实施方案中，由酵母编码的单羧酸/单羧酸盐转运蛋白具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25或SEQ ID NO:26。

在一些实施方案中，酵母以足以在乙醇滴度为至少为75g/L时，将发酵液中的海藻糖含量降低至小于0.5g/L、1g/L、2g/L、3g/L、4g/L、5g/L、6g/L、7g/L、8g/L、9g/L或10g/L的量分泌海藻糖酶。在一些实施方案中，酵母能够在细胞外分泌海藻糖酶。在一些实施方案中，由酵母编码的海藻糖酶多肽与SEQ ID NO：86具有至少76％、至少84％、至少92％或100％的序列同一性。在一些实施方案中，酵母能够以80g/L、90g/L、100g/L、110g/L、120g/L、125g/L、130g/L、135g/L或更高的滴度产生乙醇。

一方面，本公开涉及使用本文所述的任何酵母的方法。在一个实施方案中，该方法是用于生产乙醇的方法，其包括：使用经遗传修饰的酵母发酵培养基，其中所述酵母包含异源海藻糖酶基因，其中发酵结束时的乙醇滴度为至少90g/L。在一些实施方案中，该方法的发酵温度在25至45℃、25至40℃、25至35℃、30至40℃或28至38℃的范围内。在一些实施方案中，发酵结束时的乙醇滴度为至少80、90、100、110、120、130、135、140、145、150、155或160g/升。

一方面，本公开涉及经遗传修饰的酵母，其包含编码与SEQ ID NO：1(乳酸克鲁维酵母)具有至少80％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/L时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/L的量分泌海藻糖酶。一方面，本公开涉及经遗传修饰的酵母，其包含编码与SEQ ID NO：2(近平滑假丝酵母)具有至少80％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/L时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/L的量分泌海藻糖酶。一方面，本公开涉及经遗传修饰的酵母，其包含编码与SEQ ID NO：3(光滑假丝酵母)具有至少80％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/L时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/L的量分泌海藻糖酶。一方面，本公开涉及经遗传修饰的酵母，其包含编码与SEQID NO：87(稻瘟病菌)具有至少80％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/L时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/L的量分泌海藻糖酶。

在一些实施方案中，由酵母编码的海藻糖酶多肽包含与SEQ ID NO：83具有至少70％、80％、90％或95％的序列同一性的序列。在一些实施方案中，由酵母编码的海藻糖酶多肽包含与SEQ ID NO：84和/或SEQ ID NO：85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列。在一些实施方案中，酵母是经遗传修饰的酿酒酵母。在一些实施方案中，由酵母编码的海藻糖酶包含MFα2信号序列。在一些实施方案中，MFα2信号序列是SEQ ID NO：4。在一些实施方案中，MFα2信号序列与SEQ ID NO：4具有至少84％、89％或94％的序列同一性。在一些实施方案中，由酵母编码的海藻糖酶与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQID NO：8、SEQ ID NO：11、SEQ ID NO：14或SEQ ID NO：92。

在一个方面，酵母还包含编码葡糖淀粉酶(EC 3.2.1.3)多肽的异源基因。在一些实施方案中，由酵母编码的葡糖淀粉酶与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性：SEQ ID NO:16(Sf GA)、SEQ ID NO:17(Ro GA)、SEQID NO:108(Rmic GA)或SEQ ID NO:109(Rdel GA)。

在一个方面，酵母以足以在乙醇滴度为至少为110g/L时，将发酵液中的海藻糖含量降低至小于0.5g/L或1g/L的量分泌海藻糖酶。在一些实施方案中，酵母能够在细胞外分泌海藻糖酶。在一些实施方案中，由酵母编码的海藻糖酶多肽包含与SEQ ID NO：86具有至少76％、至少84％、至少92％或100％的序列同一性的序列。

在一个方面，酵母包含编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸；以及减少或消除的编码甘油-3-磷酸磷酸酶(EC 3.1.3.21)的基因的表达。在一个方面，酵母包含编码甘油醛-3-磷酸脱氢酶(GAPN，E.C.1.2.1.9)的重组核酸。在一些实施方案中，编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸编码与SEQ ID NO：111(蜡样芽孢杆菌(Bacillus cereus)GAPN)具有至少80％、85％、90％或95％的序列同一性的多肽。

在一些实施方案中，酵母能够以80g/L、90g/L、100g/L、110g/L、120g/L、125g/L、130g/L、135g/L或更高的滴度产生乙醇。在一些实施方案中，所述酵母与不表达异源海藻糖酶的酵母相比，产生更高的乙醇滴度和/或收率。在一些实施方案中，所述酵母与不表达异源海藻糖酶但在其它方面与所述酵母相同的酵母相比，产生更高的乙醇滴度和/或收率。在一些实施方案中，所述酵母与表达不同的异源海藻糖酶的酵母相比，产生更高的乙醇滴度和/或产量。

在一个方面，本公开涉及用于生产乙醇的方法，其包括：使用经遗传修饰的酵母发酵培养基，其中所述酵母包含编码与以下多肽序列中的至少一个或多个具有至少80％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源海藻糖酶基因：SEQ ID NO：1(乳酸克鲁维酵母)、SEQ ID NO：2(近平滑假丝酵母)、SEQ ID NO：3(光滑假丝酵母)或SEQ ID NO：87(稻瘟病菌)，其中如在接种后36小时所测得的，发酵结束时的乙醇滴度为至少为105g/L，并且发酵结束时发酵液的海藻糖含量小于2g/L。在一些实施方案中，所述方法的酵母包含编码与以下多肽序列中的仅一个具有至少80％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源海藻糖酶基因：SEQ ID NO：1(乳酸克鲁维酵母)、SEQ ID NO：2(近平滑假丝酵母)、SEQID NO：3(光滑假丝酵母)或SEQ ID NO：87(稻瘟病菌)。应当理解，用于生产乙醇的方法中使用的酵母可以是本文所述的酵母的任何实施方案，包括与任何其它所述的一种或多种性状或一种或多种修饰组合描述的任何性状或修饰。

在一些实施方案中，发酵温度的范围为25至45℃、25至40℃、25至35℃、30至40℃或28至38℃。在一些实施方案中，发酵结束时的乙醇滴度为至少120g/升、130g/升、135g/升、140g/升、145g/升、150g/升、155g/升或160g/升。在一些实施方案中，酵母是本文描述的任何实施方案或方面的酵母。

发酵液中乙醇和海藻糖含量的值可以根据以下实施例1中所述的摇瓶法进行评估和测量。

还应理解，如本领域技术人员将理解的，上述过程、方法或组合物的任何实施方案的要素或方面可应用于任何其他实施方案。

附图说明

当结合附图一起阅读时，将更好地理解本发明的以下详细说明。然而，应当理解，本发明并不限于在附图中所示的实施方案的精确安排和手段。

图1是显示使用不同的酵母菌株的发酵中的海藻糖浓度随时间变化的图。

图2和图3是显示不添加海藻糖的发酵中的乙醇量的图。

图4是显示添加海藻糖的发酵中海藻糖浓度随时间变化的图。

图5和图6是显示添加海藻糖的发酵中的乙醇量的图。

图7是显示添加海藻糖的发酵中海藻糖浓度随时间变化的图。

图8和图9是显示添加海藻糖的发酵中的乙醇量的图。

图10是显示使用不同酵母菌株的玉米醪发酵中的乙醇量的图。

图11是显示使用不同酵母菌株的玉米醪发酵中的海藻糖量的图。

具体实施方式

应当理解，本文提供的本发明的附图和描述已经简化，以举例说明与清楚理解本发明相关的要素，同时消除了在一个或多个相关领域中发现的其它要素。本领域普通技术人员将认识到，在实施本发明时可能期望或需要其他要素或步骤。然而，因为此类要素和步骤是本领域熟知的，或者不利于更好地理解本发明，本文不提供对此类要素和步骤的讨论。

定义

除非另外定义，否则本文所用的所有技术和科学术语都具有与本发明所属领域中的技术人员通常所理解相同的含义。如本文中所用，以下术语中的每一个均具有与其在本章节中相关的含义。

发酵方法定义

如本文中所用，“接种”被定义为将能够产生发酵产物的微生物引入发酵培养基中时的时间点。这是本领域的技术人员熟知的术语。

如本文中所用，“发酵结束”被定义为发酵方法满足预定标准时的时间点。预定标准可包括以下中的任一项：预定时间间隔、所供应的期望分数的碳源的耗尽、碳源消耗的停止或发酵产物形成的停止。在一个实施方案中，“发酵结束”被定义为开始收获生物产物时的时间点。如本领域技术人员将理解的，“发酵结束”可以指根据发酵方法的规模和目的而不同的时间点。对于大规模生产发酵方法，“发酵结束”优选是开始收获生物产物时的时间点，即在产物形成已有效停止之后。

如本文中所用，“细胞干重”是指如在发酵样品中所测量的测量时存在于发酵培养基中的干细胞质量的浓度。细胞干重通常以克/升(g/L)为单位表示。

如本文中所用，“接种时的细胞干重”是指如在发酵样品中所测量的接种后即刻的存在于发酵培养基中的干细胞质量的浓度。对于分批补料发酵，基于发酵培养基的最终体积计算初始细胞干重。干细胞重量的测量是本领域技术人员已知的方法。接种时的细胞干重通常以g/L为单位表示。

如本文中所用，“发酵结束时的细胞干重”是指如在发酵样品中所测量的发酵结束时存在于发酵培养基中的干细胞质量的浓度。发酵结束时的细胞干重通常以g/L为单位表示。

如本文中所用，“最终滴度”是指发酵结束时发酵液中物质的浓度。最终滴定度通常以g/L为单位表示。

如本文中所用，“初始滴度”是指接种时存在的物质的浓度。初始滴定度通常以g/L为单位表示。

如本文中所用，“批次时间”是指在接种和发酵结束之间经过的时间量。批次时间通常以小时(h)为单位表示。

如本文中所用，用于分批过程的“发酵生产速率”是指发酵产物的最终滴度减去初始滴度(最终滴度减去初始滴度)再除以批次时间。生产速率通常以克/升-小时(g L^-1h^-1)为单位表示。当应用于连续或半连续过程时，使用本领域已知的方法测定“发酵生产速率”。

如本文中所用，“比生产速率”是指发酵生产速率除以发酵结束时的细胞干重。比生产速率通常以(g产物)(g细胞)^-1h^-1为单位表示。当应用于连续或半连续过程时，使用本领域已知的方法测定“比生产速率”。

如本文中所用，发酵产物的“产物收率”是指两个量的比率：a)发酵方法中产生的产物(例如，琥珀酸酯)的质量(分子)，b)添加至发酵中的碳源的质量(分母)。产物收率通常以克/克(g/g)乘以100为单位表示。应特别注意的是，产物收率是以质量比计算的。产生的发酵产物的质量应考虑到批次结束时发酵培养基中存在的发酵产物的质量，以及批次过程中收获的任何发酵产物的质量，减去批次开始时存在的发酵产物的质量，以及进一步减去在批次过程中添加的任何发酵产物的质量。除了在批次过程中添加的任何一种或多种碳源的质量外，添加到批次中的碳源的质量还应包括批次开始时发酵罐中存在的所有碳源的质量。

如本文中所用，“耗氧速率”(“OUR”)是指发酵期间消耗氧气的体积速率。入口和出口的氧气浓度可通过废气分析，例如通过质谱仪来测量。可由相关领域的普通技术人员使用在Bioreaction Engineering Principles第2版，2003,Kluwer Academic/PlenumPublishers，第449页，方程式1中描述的直接方法来计算OUR。其通常以(mmol O₂)L^-1h^-1为单位来测量。

如本文中所用，“比耗氧速率”是指发酵期间消耗氧气的比速率。其计算为OUR与测得的细胞干重之比。其通常以mmol O₂(g细胞干重)^-1h^-1为单位来测量。

酵母特征定义

术语“经遗传修饰的”和“经遗传工程改造的”在本文可互换使用，并且是指生物体的遗传物质的任何改变，或指如此改变的生物体。对于本公开的目的，这些术语不意味着受改变方法的限制。

在某些实施方案中，本文提供的经遗传修饰的酵母细胞还包含一种或多种天然基因的缺失或破坏。如本文中所用，关于天然基因的短语“缺失或破坏”是指基因的整个编码区被消除(缺失)或基因的编码区、其启动子和/或其终止子区被修饰(诸如通过缺失、插入或突变)，从而使得基因不再产生活性酶，产生大大减少的量(至少75％的减少，优选至少90％的减少)的活性酶，或产生活性大大降低(降低至少75％，优选降低至少90％)的酶。

在某些实施方案中，一种或多种天然基因的缺失或破坏导致一种或多种天然代谢途径的缺失或破坏。关于代谢途径的短语“缺失或破坏”是指该途径是无效的或另外地表现出相对于天然途径降低了至少75％、至少85％或至少95％的活性。

在一些实施方案中，天然基因的缺失或破坏可以通过强制进化、诱变或遗传工程改造方法，随后进行适当的选择或筛选以鉴定所需的突变体来完成。在一些实施方案中，天然宿主细胞基因的缺失或破坏可与一种或多种外源基因至宿主细胞中的整合偶联，即，可使用也是缺失构建体的基因表达整合构建体整合外源基因。在一些实施方案中，可使用不含外源基因的缺失构建体或通过本领域已知的其它方法来完成缺失或破坏。

如本文中所用，关于遗传组分的术语“异源的”是指遗传组分存在于微生物的修饰形式中，但不存在于特定微生物细胞的天然形式的基因组中。在一些实施方案中，异源遗传组分可以是细胞天然组分的修饰形式，其可源自另一生物，其可以是源自另一生物的组分的修饰形式，或者其可以是合成来源的组分。在优选的实施方案中，将异源遗传组分整合到经修饰的微生物的基因组中。例如，乳酸克鲁维酵母海藻糖酶基因在引入酿酒酵母中时是异源的。

如本文中所用，术语“外源的”是指源自目标微生物之外的任何材料。例如，术语“外源的”可应用于在遗传修饰之前不以特定生物的天然形式存在的遗传物质(即，这种外源遗传物质也可以称为异源的)，或者其也可被应用于并非源自特定生物体的酶或其它蛋白质。

检查两种核酸或两种多肽的核酸或氨基酸序列将揭示所比较的序列之间的序列同一性和相似性。序列比对和序列同一性的产生包括通常使用计算方法进行的全局比对和局部比对。可使用BLAST(国家生物信息中心(NCBI)基本局部比对搜索工具)2.2.31版软件，利用默认参数进行比对。氨基酸序列之间的氨基酸序列同一性％可使用利用以下默认参数的标准蛋白质BLAST来测定：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：6；查询范围内的最大匹配数：0；矩阵：BLOSUM62；空位成本(Gap Cost)：(存在：11，延伸：1)；组成调整：条件式组成得分矩阵调整；过滤器：没有选择；掩码：没有选择。核酸序列之间的核酸序列同一性％可使用利用以下默认参数的标准核苷酸BLAST来测定：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：28；查询范围内的最大匹配数：0；匹配/不匹配分数：1，-2；空位成本：线性；过滤器：低复杂度区域；掩码：仅用于查找表的掩码。使用利用默认参数的NCBI BLAST 2.2.31版算法相对于参考序列具有XX％(例如，80％)的同一性分数的序列被认为与参考序列至少XX％相同或等同、与参考序列具有XX％的序列同一性。

贯穿本公开，本发明的各个方面可以以范围的形式呈现。应当了解，以围形式呈现的描述仅仅是为了方便和简洁，并且不应理解为对本发明范围的硬性限制。因此，范围的描述应被认为具有确切公开的所有可能的子范围以及该范围内的单独数值。例如，对诸如1至7的范围的描述应被认为具体公开了诸如1至3、1至4、1至6、2至5、3至5等的子范围，以及该范围内的各个数字，例如1、2、3、3.6、4、5、5.8、6、7和其间的任何整数及分数增量。不管范围有多宽，这都适用。

描述

本文描述了可用于生产发酵产物的经遗传修饰的酵母菌株和使用这些酵母的发酵方法。酵母菌株经修饰以包括一种或多种异源海藻糖酶基因。在一些实施方案中，酵母菌株还可包括其它异源基因，例如异源葡糖淀粉酶基因，而对发酵方法中所需的异源酶表达水平和/或酵母性能没有任何显著不利影响。

遗传工程改造的酵母

海藻糖酶是催化海藻糖向葡萄糖转化的糖苷水解酶。在一个方面，本文所述的遗传工程改造的(GE)酵母已被修饰为包括异源海藻糖酶基因。在一些实施方案中，GE酵母由酿酒酵母宿主酵母细胞产生。在一些实施方案中，宿主酵母细胞是适合于乙醇生产的酵母菌株，例如Ethanol Red^TM或酿酒酵母的类似菌株。因此，在一些实施方案中，GE酵母耐受乙醇发酵方法中使用的条件，诸如相对高的温度和/或乙醇浓度。在一些实施方案中，与宿主细胞相比，包含异源海藻糖酶基因可改善热和/或乙醇耐受性。

在一些实施方案中，GE酵母可包含用于表达来自以下物种中的一种或多种的海藻糖酶多肽的一种或多种基因：乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母。在一些实施方案中，GE酵母表达与以下氨基酸序列中的至少一个具有至少70％、75％、80％、85％、90％、95％或97％的序列同一性的海藻糖酶多肽：SEQ ID NO：1(乳酸克鲁维酵母)、SEQ IDNO：2(近平滑假丝酵母)或SEQ ID NO：3(光滑假丝酵母)。在一些实施方案中，GE酵母表达来自稻瘟病菌的海藻糖酶多肽(SEQ ID NO：87)。在一些实施方案中，GE酵母表达与SEQ IDNO:87具有至少70％、75％、80％、85％、90％、95％或97％的序列同一性的海藻糖酶多肽。在一个方面，GE酵母可包括过表达的天然海藻糖酶基因而非异源海藻糖酶基因，或者除了异源海藻糖酶基因以外还包括过表达的天然海藻糖酶基因，例如，源自酿酒酵母宿主酵母的GE酵母中的过表达的酿酒酵母海藻糖酶基因。对于本公开和权利要求的目的，任何过表达的天然基因和由这样的基因编码的任何多肽都将被认为是异源的。

在一些实施方案中，GE酵母可包含用于表达来自以下物种中的一个或多个物种的海藻糖酶多肽的一种或多种基因，其中每个物种的相关多肽序列都包含在物种名称后的括号中：酿酒酵母(SEQ ID NO：42)、德尔布有孢圆酵母(Torulaspora delbrueckii)(SEQ IDNO：43)、西氏哈萨克酵母(Kazachstania naganishii)(SEQ ID NO：44)、蟑螂四盾酵母(Tetrapisispora blattae)(SEQ ID NO：45)、鲁氏酵母(Zygosaccharomyces rouxii)(SEQID NO：46)、副贝氏丝酵母(Zygosaccharomyces parabailii)(SEQ ID NO：47)、毕赤四盾酵母(Tetrapisispora phaffii)(SEQ ID NO：48)、棉假囊酵母(Eremothecium gossypii)(SEQ ID NO：49)、鼻窦假囊酵母(Eremothecium sinecaudum)(SEQ ID NO：50)、米拉氏拉茜斯酵母(Lachancea mirantina)(SEQ ID NO：51)、近平滑假丝酵母(Candidaorthopsilosis)(SEQ ID NO：52)、麦芽糖假丝酵母(Candida maltose)(SEQ ID NO：53)、热带假丝酵母(Candida tropicalis)(SEQ ID NO：54)、白假丝酵母(SEQ ID NO：55)、长孢洛德酵母(Lodderomyces elongisporus)(SEQ ID NO：56)、杜氏假丝酵母(Candidadubliniensis)(SEQ ID NO：57)、木糖发酵酵母(Spathaspora passalidarum)(SEQ ID NO：58)、树干毕赤酵母(Scheffersomyces stipitis)(SEQ ID NO：59)、法布里德巴利酵母(Debaryomyces fabryi)(SEQ ID NO：60)、坦扎瓦丝假丝酵母(Candida tanzawaensis)(SEQ ID NO：61)、多布克鲁维酵母(Kluyveromyces dobzhanskii)(SEQ ID NO：62)、马克斯克鲁维酵母(Kluyveromyces marxianus)(SEQ ID NO：63)、鲁氏接合酵母(Zygosaccharomyces rouxii)(SEQ ID NO：64)、达氏诺莫维酵母(Naumovozymadairenensis)(SEQ ID NO：65)、耐热拉茜斯酵母(Lachancea thermotolerans)(SEQ IDNO：66)、魁北克拉茜斯酵母(Lachancea quebecensis)(SEQ ID NO：67)、毕赤四盾酵母(SEQID NO：68)、发酵拉茜斯酵母(Lachancea fermentati)(SEQ ID NO：69)、假山毛榉拉茜斯酵母(Lachancea nothofagi)(SEQ ID NO：70)、蟑螂四盾酵母(SEQ ID NO：71)、小麦顶囊壳(Gaeumannomyces tritici)(SEQ ID NO：72)、夏季斑枯病菌(Magnaporthiopsis poae)(SEQ ID NO：73)、嗜热毁丝霉(Thermothelomyces thermophila)(SEQ ID NO：74)、若虫炭疽菌(Colletotrichum nymphaeae)(SEQ ID NO：75)、兰花炭疽菌(Colletotrichumorchidophilum)(SEQ ID NO：76)、木生锥毛壳(Coniochaeta ligniaria)(SEQ ID NO：77)、土生梭抱壳霉(Thielavia terrestris)(SEQ ID NO：78)、足马杜拉分枝菌(Madurellamycetomatis)(SEQ ID NO：79)、粗糙脉孢菌(Neurospora crassa)(SEQ ID NO：80)、大丽轮枝菌(Verticillium dahlia)(SEQ ID NO：81)和/或玉蜀黍赤霉(Gibberella zeae)(SEQID NO：82)。GE酵母可表达与这些氨基酸序列中的任何一个或多个氨基酸序列具有至少70％、75％、80％、85％、90％、95％或97％的序列同一性的海藻糖酶多肽。

在一些实施方案中，GE酵母编码来自一种物种的异源海藻糖酶多肽，所述物种包括特征模式或基序，即，与来自另一物种的海藻糖酶的氨基酸亚组相同或几乎相同的海藻糖酶多肽的全序列中的氨基酸亚组。例如，来自SEQ ID NO：1(乳酸克鲁维酵母)、SEQ IDNO：2(近平滑假丝酵母)以及SEQ ID NO：3(光滑假丝酵母)的海藻糖酶的每一种包含以下氨基酸基序：QPYVANGYIGSRIPN(SEQ ID NO:83)。在一些实施方案中，GE酵母表达与SEQ IDNO：83具有至少70％、至少80％、至少85％、至少90％或100％的序列同一性的海藻糖酶多肽。另外，来自SEQ ID NO：1(乳酸克鲁维酵母)、SEQ ID NO：2(近平滑假丝酵母)以及SEQ IDNO：3的海藻糖酶的每一种以大于90％的序列同一性包含两个以下基序：GVAGLSSDSYGGMVFWD(SEQ ID NO:84)和NITLEYSGMNSSVEIKQADV(SEQ ID NO:85)。在一些实施方案中，GE酵母表达具有与SEQ ID NO:84和/或SEQ ID NO:85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列的一部分的海藻糖酶多肽。以下氨基酸序列包含在大多数或所有酸性海藻糖酶中：NITLEYSGMNSSV(SEQ IDNO:86)。在一些实施方案中，GE酵母表达具有与SEQ ID NO：86具有至少76％、至少84％、至少92％或100％的序列同一性的序列的一部分的海藻糖酶多肽。已知酿酒酵母具有表达两种类型的海藻糖酶(酸性海藻糖酶(AT)和中性海藻糖酶(NT))的天然海藻糖酶基因，所述海藻糖酶根据最佳的表达pH来表征。然而，两种天然海藻糖酶均受到严格调节并且显示出低活性。在低应激或无应激条件下，几乎不产生这些海藻糖酶。另外，这些海藻糖酶可被限制在液泡中。(关于酿酒酵母海藻糖酶的论述，参见，例如，Parrou,J.L.,Jules,M.,Beltran,G.,J.Acid trehalase in yeasts and filamentous fungi:Localization,regulation and physiological function(2005)FEMS Yeast Research,5(6-7)，第503-511页；Eleutherio,E.,Panek,A.,De Mesquita,J.F.,Trevisol,E.,/>R.Revisiting yeast trehalose metabolism(2015)Current Genetics,61(3)，第263-274页)。

Zilli等描述了光滑假丝酵母海藻糖酶在酵母属中的异源表达(Zilli,D.M.W.,Lopes,R.G.,Alves,S.L.,Barros,L.M.,Miletti,L.C.,Stambuk,B.U.Secretion of theacid trehalase encoded by the CgATH1gene allows trehalose fermentation byCandida glabrata(2015)Microbiological Research,179，第12-19页)。然而，包括光滑假丝酵母海藻糖酶的Zilli中的酵母菌株产生极低量的乙醇，即，不适用于商业过程的乙醇的量。本发明的GE酵母可以产生显著更高的商业上可用的乙醇量。

在一个方面，本发明的GE酵母表达海藻糖酶，其在细胞外大量分泌，而不是如在天然酿酒酵母的海藻糖酶中看到的那样在液泡中表达并束缚在其中。因此，表达的海藻糖酶可作用于发酵液中的细胞外海藻糖，即在发酵方法中海藻糖作为酵母的代谢物产生。在一些实施方案中，GE酵母比野生型酵母分泌更多的海藻糖酶，但是不产生对酵母造成显著的代谢负担的量的海藻糖酶，即，酵母产生足够的海藻糖酶来消耗细胞产生的大部分或全部海藻糖而不会引起负面影响发酵性能的其它问题。在一些实施方案中，酵母可包括启动子，所述启动子与防止酵母产生对细胞造成代谢负担或以其它方式负面影响发酵性能的量的海藻糖酶相关联。

对于本公开的目的，在一个方面，海藻糖酶是来自EC 3.2.1.28的酶。因此，海藻糖酶是其主要活性为水解海藻糖的任何酶。在一个方面，海藻糖酶是在合理的时间范围(timeframe)内对海藻糖表现出显著活性的任何酶。

在一个方面，可通过包含肽信号序列(即前导序列)来优化或改善海藻糖酶在GE酵母中的表达，所述前导序列不同于与某些海藻糖酶相关联的野生型前导序列。在一些实施方案中，信号肽是MFα2信号序列。在一个方面，本公开的表达的海藻糖酶蛋白可包含与SEQID NO：4具有约79％或更高、84％或更高、89％或更高或94％或更高序列同一性的信号序列，所述信号序列源自酿酒酵母交配因子α2基因(Sc MFα2)的N端。在一些实施方案中，ScMFα2SS序列如下：MKFISTFLTFILAAVSVTA(SEQ ID NO:4)。Sc MFα2序列来自基因YGL089C(YGL089C)，而MFα1由基因YPL187W MFα1编码，并且MFα2是MATa细胞分泌的信息素。ScMFα2-分泌信号修饰的海藻糖酶多肽及表达其的工程改造的酵母菌株描述于2016年2月5日提交的国际专利申请序列号PCT/US2016/016822(Miller，等)中。酿酒酵母交配因子α2(ScMFα2)分泌信号描述于美国专利第4,546,082号(Kurjan等)中。在一些实施方案中，酵母包含用于表达由酵母编码的海藻糖酶的基因，所述海藻糖酶与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQ ID NO：8、SEQ ID NO：11、SEQ IDNO：14或SEQ ID NO：92(即，具有Sc MFα2而非其天然分泌信号的海藻糖酶)。

在一些实施方案中，MFα2分泌信号是与SEQ ID NO：8的多肽序列具有至少80％、85％、90％或95％的序列同一性的乳酸克鲁维酵母酸性海藻糖酶分泌信号。在一些实施方案中，MFα2分泌信号是与SEQ ID NO：11的多肽序列具有至少80％、85％、90％或95％的序列同一性的近平滑假丝酵母酸性海藻糖酶分泌信号。在一些实施方案中，MFα2分泌信号是与SEQ ID NO：14的多肽序列具有至少80％、85％、90％或95％的序列同一性的光滑假丝酵母酸性海藻糖酶分泌信号。

本公开并不意味着限于任何特定的海藻糖酶多肽，并且GE酵母可包括表达可用于发酵方法的任何海藻糖酶多肽的基因。在一些实施方案中，将酸性海藻糖酶基因整合到GE酵母中。在一些实施方案中，将中性海藻糖酶基因整合到GE酵母中。在一些实施方案中，GE酵母可包含酸性海藻糖酶基因和中性海藻糖酶基因。如本领域技术人员将理解的，可以根据发酵液的预期pH、发酵方法的其它特征和/或GE酵母本身的其它特征选择启动子或前导序列来优化酸性海藻糖酶和/或中性海藻糖酶的表达。

另外，本公开并不意味着限于整合到GE酵母中的一种或多种海藻糖酶基因的任何特定的前导序列。本文所述的任何分泌信号序列可与本文所述的任何海藻糖酶多肽一起使用，即，本公开意在包括前导序列和海藻糖酶多肽的每种组合。因此，GE酵母可包括与GE酵母本身异源的海藻糖酶的前导序列。这样的前导序列可以是与异源海藻糖酶相关联的野生型，或者对于GE酵母和从中获取海藻糖酶的物种都可以是异源的。在一些实施方案中，GE酵母中不同基因的天然前导序列可以与异源海藻糖酶一起使用。

对于本公开的目的，当鉴定序列与任何海藻糖酶多肽的序列同一性百分比时，应理解天然前导序列氨基酸不包括在序列同一性的计算中。然而，如果前导序列氨基酸不能使用此类方法来容易且完全确定，则使用全长海藻糖酶多肽序列(即包括天然前导序列)来计算序列同一性百分比。例如，SEQ ID NO：87是具有其天然前导序列的稻瘟病菌海藻糖酶的多肽，而SEQ ID NO：88是没有其天然前导序列的稻瘟病菌海藻糖酶的多肽。没有分泌前导序列的海藻糖酶多肽的其它实例包括SEQ ID NO：89(光滑假丝酵母)、SEQ ID NO：90(近平滑假丝酵母)和SEQ ID NO：91(乳酸克鲁维酵母)。因此，在一些实施方案中，GE酵母编码与SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90或SEQ ID NO:91具有至少75％、至少80％、至少90％、至少95％、至少97％或100％的序列同一性的异源海藻糖酶多肽。在一些实施方案中，GE酵母编码异源海藻糖酶多肽，所述异源海藻糖酶多肽与以下代表具有MFα2分泌信号的海藻糖酶多肽的序列中的任一个具有至少75％、至少80％、至少90％、至少95％、至少97％或100％的序列同一性：SEQ ID NO：4与SEQ ID NO：88的组合、SEQ ID NO：4与SEQ IDNO：89的组合、SEQ ID NO：4与SEQ ID NO：90的组合或SEQ ID NO：4与SEQ ID NO：91的组合。

尽管不希望受到理论束缚，但序列分析软件仍预测乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母海藻糖酶全部三种的天然前导序列(信号肽)是跨膜结构域，而不是分泌信号。这表明海藻糖酶被从细胞膜推出到周质空间(即细胞膜和细胞壁之间的空间)中。然而，这些海藻糖酶可能仍锚定在细胞膜上。用MFα2分泌信号替代天然信号肽可能会将所述蛋白质从膜上解脱出来，这使得能够实现细胞外分泌。

He等(He,S.,Bystricky,K.,Leon,S.,J.M.,Parrou,J.L.TheSaccharomycescerevisiae vacuolar acid trehalase is targeted at the cell surface for itsphysiological function(2009)FEBS Journal,276(19)，第5432-5446页)描述了用来自2个其它基因的分泌前导序列替代天然酵母属酸性海藻糖酶信号肽。然而，无论该研究中的信号肽如何，大量的蛋白质似乎都在液泡中。

在一个方面，可通过包含启动子来优化或改善海藻糖酶在GE酵母中的表达。在一些实施方案中，启动子是TDH3启动子。在一个实施方案中，启动子是与SEQ ID NO：5具有至少80％、85％、90％或95％的序列同一性的酿酒酵母TDH3启动子。在一些实施方案中，启动子是SAM2启动子。在一些实施方案中，启动子是与SEQ ID NO：112具有至少80％、85％、90％或95％的序列同一性的酿酒酵母SAM2启动子。

在一个方面，GE酵母表达海藻糖酶解决了通常与用于通过发酵生产生物产物的GE酵母相关的重大问题。酵母在受到应激时通常会产生更多的海藻糖。一些发酵方法条件可在用于生物产物生产的GE酵母中造成应激。例如，一些发酵方法与高温相关，这导致对酵母的应激。高乙醇或其它生物产物浓度和/或高盐浓度也会造成增加海藻糖产生的应激。GE酵母中异源酶的表达还可导致海藻糖产生的增加，因为这种酶的表达可造成对酵母的应激。特别地，已知表达葡糖淀粉酶的工程改造的酵母表现出更高的海藻糖产生。

然而，本发明的表达海藻糖酶的酵母可以通过减少或消除由用于生物产物发酵的酵母产生的海藻糖来解决该问题。海藻糖被转化为葡萄糖，并且可以被酵母用作用于代谢需要和/或生物产物形成的碳源。因此，被酵母用于产生海藻糖的碳可以被酵母有效地再循环以制备蛋白质或生物产物，从而改善了使用酵母的发酵方法的总体性能。

在一些研究中，与本公开的教导相反，已在酵母中缺失或破坏天然海藻糖酶基因以试图减少应激问题并增加乙醇耐受性(参见，例如，Trevisol,E.T.V.,Panek,A.D.,Mannarino,S.C.,Eleutherio,E.C.A.,The effect of trehalose on the fermentationperformance of aged cells of Saccharomyces cerevisiae(2011)AppliedMicrobiology and Biotechnology,90(2)，第697-704页，其论述了酸性海藻糖酶(ATH1)或中性海藻糖酶(NTH1)的缺失以及由此增强的乙醇耐受性)。然而，令人惊奇地显示，与野生型或其它工程改造的酵母相比，分泌异源海藻糖酶的本发明的GE酵母可改善乙醇生产性能。

在一些实施方案中，GE酵母还可包含用于表达除海藻糖酶外的多肽的异源基因。在一些实施方案中，GE酵母可包括用于表达以下的任一种或全部的一个或多个异源基因：淀粉酶，例如葡糖淀粉酶(EC 3.2.1.3)、与乳酸消耗相关的蛋白质(例如，编码单羧酸/单羧酸盐转运蛋白的异源基因和/或一个或多个编码乳酸脱氢酶(细胞色素)(分类为EC1.1.2.3或1.1.2.4)的异源基因))、异麦芽糖酶(EC 3.2.1.10)和糖转运蛋白。在一些实施方案中，GE酵母还可包含用于优化此类多肽的表达的一种或多种启动子和/或前导序列。在一些实施方案中，GE酵母可包含2个或更多个拷贝的本文所述的任何异源基因。

葡糖淀粉酶(E.C.3.2.1.3)是淀粉分解酶，其从寡糖链和多糖链的非还原性末端相继水解1,4-连接的a-D-葡糖基残基并释放D-葡萄糖。在一些实施方案中，GE酵母编码异源海藻糖酶和异源葡糖淀粉酶两者。

在以下参考文献中进一步描述了上述遗传修饰，所述参考文献的全部内容由此通过引用整体并入：2016年2月5日提交的WO 2016/127083(MODIFIED GLUCOAMYLASE ENZYMESAND YEAST STRAINS HAVING ENHANCED ETHANOL PRODUCTION)；2016年3月25日提交的WO2016/160584(GLUCOAMYLASE-MODIFIED YEAST STRAINS AND METHODS FORBIOPRODUCTPRODUCTION)；2016年12月16日提交的PCT/US16/067314(公布为WO 2017/106739)(SUGAR TRANSPORTER-MODIFIED YEAST STRAINS AND METHODS FOR BIOPRODUCTPRODUCTION)；2016年8月5日提交的美国专利申请62/371,681(公布为WO 2018/027131)(LEADER-MODIFIED GLUCOAMYLASE POLYPEPTIDES AND ENGINERED YEAST STRAIN HAVINGENHANCED BIOPRODUCT PRODUCTION)；2016年9月16日提交的美国专利申请62/395,792(公布为WO 2018/053230)(GENETICALLY MODIFIED LACTATE-CONSUMING YEASTS ANDFERMENTATION PROCESSES USING SUCH GENETICALLY MODIFIED YEASTS)。

在一些实施方案中，GE酵母可包含具有以下SEQID和/或表达以下多肽SEQID中的任一个或多个的基因，其包括以上参考文献中描述的遗传修饰的实施方案：与SEQ ID NO:16(扣囊复膜孢酵母)葡糖淀粉酶(GA)多肽的氨基酸19-515)、SEQ ID NO:17(与米根霉GA多肽的氨基酸26-604)、SEQ ID NO:18(白宇佐美曲霉GA多肽的氨基酸19-639)和/或SEQ IDNO:19(土曲霉(Aspergillus terreus)GA多肽的氨基酸21-636)具有大于50％、60％、70％、75％、80％、85％、90％或95％的序列同一性；与SEQ ID NO:108(小孢根霉(Rhizopusmicrosporus)GA多肽)具有大于75％、80％、81％、85％、90％或95％或更高的序列同一性；与SEQ ID NO:109(戴尔根霉(Rhizopus delemar)GA多肽)具有大于80％、85％、90％、95％或97％或更高的序列同一性；与SEQ ID NO:20(米卡塔酵母(Saccharomyces mikatae)糖转运多肽)具有大于80％、85％、90％或95％或更高的序列同一性；与SEQ ID NO:21(酿酒酵母MAL11)具有大于50％、60％、70％、80％、85％、90％或95％或更高的序列同一性；与以下氨基酸序列中的至少一个具有至少65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25,或SEQ ID NO:26(异源单羧酸盐/质子同向转运蛋白氨基酸，例如JEN1同向转运蛋白，来自东方伊萨酵母(Issatchenkia orientalis)、酿酒酵母、乳酸克鲁维酵母、多布克鲁维酵母或马克斯克鲁维酵母)；具有与以下氨基酸序列中的至少一个具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列的多肽：SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32或SEQ ID NO:33(来自酿酒酵母、东方伊萨酵母、克鲁弗酵母(Saccharomyces kluyveri)、贝酵母(Saccharomyces bayanus)、鲁氏接合酵母(Zygosaccharomyces rouxii)、乳酸克鲁维酵母或多布克鲁维酵母的细胞色素b2(CYB2)多肽)；或与以下氨基酸序列中的至少一个具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ IDNO:38、SEQ ID NO:39、SEQ ID NO:40或SEQ ID NO:41(来自酿酒酵母、东方伊萨酵母、克鲁弗酵母、贝酵母、烟曲霉、乳酸克鲁维酵母、多布克鲁维酵母或马克斯克鲁维酵母的D-乳酸脱氢酶(DLD)多肽)。在一个方面，Lys349、Tyr143、Tyr254和His373的残基和相关位置在SEQID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32或SEQ ID NO:33中是保守的。由GE酵母编码的任何上述SEQ ID可以是如上文中定义的异源的。

如本领域技术人员将理解的，修饰宿主酵母以包含多个异源基因可导致对宿主酵母的不可预测的作用。例如，含有多个异源基因(即2个或更多个表达不同类别的酶或其它蛋白质的异源基因)的GE酵母可对酵母产生负面的代谢作用，不利地影响酵母的耐热性，或不利地影响酵母产生的乙醇量。然而，令人惊讶地发现，在本文所述的至少一些实施方案中，包含异源海藻糖酶基因对GE酵母没有任何明显的不利作用。相反，与不包含所有多个性状的酵母相比，包含多个性状(即，多个不同的异源基因)的GE酵母在乙醇发酵方面的性能更好。

在一个方面，已经发现，与未修饰的宿主细胞相比，在酵母中包含一种或多种表达异源酶的基因，例如表达葡糖淀粉酶的基因可导致酵母的海藻糖产量增加。另外，还发现与突变前的宿主细胞相比，通过诱变进行遗传修饰以改善耐热性、乙醇耐受性或其它特征的酵母显示出增加的海藻糖产量。然而，与未修饰的宿主细胞或除包含整合的异源海藻糖基因外具有所有相同修饰的宿主细胞相比，本发明的GE酵母可显示出较低的海藻糖产量。在一些实施方案中，即使当将本发明的GE酵母与不含异源海藻糖酶的酵母进行比较时海藻糖的产量是相似的，表达海藻糖酶的GE酵母也可通过能够将大多数或全部产生的海藻糖转化成葡萄糖(所述葡萄糖可被进一步转化成乙醇和/或被酵母用于代谢)来产生更多乙醇。

另外，在一个方面，GE酵母可减少或消除向发酵中添加某些外源酶的需要，这些外源酶即如由本文所述的酵母表达任何酶，诸如海藻糖酶和葡糖淀粉酶，从而引起显著的成本节省。

在一个方面，由GE酵母表达海藻糖酶可引起在发酵期间更高的乙醇产量。在一些实施方案中，GE酵母可比不表达异源海藻糖酶的酵母产生多至少0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1.0％、1.1％、1.2％、1.3％、1.4％或1.5％的乙醇。在一些实施方案中，表达异源海藻糖酶的GE酵母可以以至少70g/L、75g/L、80g/L、85g/L、90g/L、95g/L、100g/L、105g/L、110g/L、115g/L、120g/L、125g/L、130g/L或135g/L或更大的滴度产生乙醇。在一些实施方案中，酵母是表达海藻糖酶的GE酵母，并且可用于使纤维素或半纤维素培养基发酵。在此类实施方案中，乙醇滴度可以低于其它乙醇生产过程中的滴度，并且仍然是商业上可行的过程。例如，这种表达海藻糖酶的酵母可以产生至少40g/L、45g/L、50g/L、55g/L、60g/L或65g/L的乙醇滴度。

在一个方面，GE酵母表达足够量的海藻糖酶，以在发酵结束时将至少25％、33％、50％、60％、70％、80％或90％的产生的或以其它方式存在于发酵液中的海藻糖转化为葡萄糖。在一个方面，GE酵母表达足够量的海藻糖酶以在发酵结束时将发酵液中的海藻糖的量减少至小于0.5g/L、1g/L、1.5g/L、2g/L、2.5g/L、3g/L、3.5g/L、4g/L、4.5g/L、5g/L、6g/L、7g/L、8g/L、9g/L或10g/L。在一些实施方案中，GE酵母表达足够量的海藻糖酶以将发酵液中的海藻糖的量减少至小于0.5g/L、1g/L、1.5g/L、2g/L、2.5g/L、3g/L、3.5g/L、4g/L、4.5g/L、5g/L、6g/L、7g/L、8g/L、9g/L或10g/L而无需向发酵中添加外源海藻糖酶，也就是说，将所述海藻糖的量降至此类水平所必需的所有海藻糖酶由酵母菌分泌。在一些实施方案中，当乙醇滴定度为至少40g/L、45g/L、50g/L、55g/L、60g/L、65g/L、70g/L、75g/L、80g/L、85g/L、90g/L、95g/L、100g/L、105g/L、110g/L、115g/L、120g/L、125g/L、130g/L或135g/L时，GE酵母可将海藻糖酶的量减少至前述水平中的任何水平。在一个方面，GE酵母表达足够量的海藻糖酶，以在发酵方法结束前转化总共至少1g/L、2g/L、3g/L、4g/L、5g/L、6g/L、7g/L、8g/L、9g/L、10g/L或更多的在发酵方法中产生和/或存在的海藻糖。尽管设想了由GE酵母产生的大多数海藻糖酶被分泌到细胞外以作用于酵母细胞外的海藻糖，即存在于发酵液中的海藻糖，但也设想了由GE酵母产生的一部分海藻糖酶可保留在细胞中，其可在所述细胞中作用于细胞内的海藻糖。在一个方面，GE酵母可包含与减少量的副产物(包括甘油)相关的遗传修饰。这些遗传修饰(或遗传修饰的组合)在本文中可以称为“甘油还原性状”。在一个方面，GE酵母包含以下修饰：编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸，以及减少或消除的编码甘油-3-磷酸磷酸酶(E.C.3.1.3.21)的基因的表达。在美国申请号62/648,679(其由此通过引用整体并入)中进一步描述了这些修饰。在一个方面，GE酵母包含以下修饰：编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸。

本文所述的工程改造的酵母菌株可包含参与甘油生产的一种或多种酶的遗传修饰。例如，本文描述的工程改造的酵母菌株可具有减少或消除的一种或多种编码甘油-3-磷酸磷酸酶(Gpp；对应于E.C.3.1.3.21；也称为“甘油-1-磷酸酶”)的基因的表达。甘油-3-磷酸磷酸酶将甘油-3-磷酸水解为甘油，从而调节甘油-3-磷酸(葡萄糖、脂质和能量代谢的代谢中间产物)的细胞水平(Mugabo等，PNAS(2016)113:E430-439)。

酿酒酵母(酿酒酵母)具有两种甘油-3-磷酸磷酸酶旁系同源物，分别称为Gpp1p和Gpp2p，分别由GPP1(UniProt号P41277)和GPP2(UniProt号P40106)基因编码(Norbeck等(1996)J.Biol.Chem.10271(23):13875-81；Pahlman等(2001)J.Biol.Chem.276(5):3555-63)。在一些实施方案中，GE酵母具有减少或消除的GPP1的表达。在其它实施方案中，GE酵母具有减少或消除的GPP2的表达。在其它实施方案中，GE酵母具有减少或消除的GPP1和GPP2两者的表达。

应当理解，实现减少或消除的编码甘油-3-磷酸磷酸酶的基因的表达的任何手段都与本发明的方面相容。例如，可以通过破坏基因和/或一个或多个调控区(控制该基因表达)的序列，诸如通过将一个或多个突变或插入引入到基因的序列中或一个或多控制该基因表达的调控区中，来实现减少或消除的编码甘油-3-磷酸磷酸酶的基因的表达。

在一些实施方案中，编码甘油-3-磷酸磷酸酶的基因，例如GPP1基因的表达减少至少约10％、20％、30％、40％、50％、60％、70％、80％、90％或100％。在一些实施方案中，编码甘油-3-磷酸磷酸酶的基因，诸如GPP1基因的表达被消除。在一些实施方案中，编码甘油-3-磷酸磷酸酶的基因，诸如GPP1基因的表达可通过本领域普通技术人员已知的任何手段，例如通过将核酸片段插入到GPP1基因座或或围绕GPP1基因座的调控区中来消除。

在一些实施方案中，GE酵母是二倍体并且具有减少或消除的两个拷贝的GPP1基因的表达。在一些实施方案中，GE酵母是二倍体并且在两个拷贝的GPP1基因中都包含缺失和/或插入。

本文所述的工程改造的酵母可具有减少或消除的一种或多种编码甘油醛-3-磷酸脱氢酶(Gpd；对应于E.C.1.2.1.12)的基因的表达。酿酒酵母具有两种甘油醛-3-磷酸脱氢酶，称为Gpd1p和Gpd2p，分别由GPD1(UniProt号Q00055)和GPD2(UniProt号P41911)基因编码。在一些实施方案中，GE酵母具有减少或消除的GPD1的表达。在其它实施方案中，GE酵母具有减少或消除的GPD2的表达。在其它实施方案中，GE酵母具有减少或消除的GPD1和表达GPD2两者的表达。

应当理解，实现减少或消除的编码甘油醛-3-磷酸脱氢酶的基因的表达的任何手段都与本发明的方面相容。例如，减少或消除的编码甘油醛-3-磷酸脱氢酶的基因的表达可通过破坏基因的序列和/或控制该基因表达的一个或多个调控区，例如通过将一个或多个突变或插入引入到基因序列中或控制所述基因表达的一个或多个调控区中来实现。

在一些实施方案中，编码甘油醛-3-磷酸脱氢酶的基因，诸如GPD1基因的表达减少至少约10％、20％、30％、40％、50％、60％、70％、80％、90％或100％。在一些实施方案中，编码甘油醛-3-磷酸脱氢酶的基因，诸如GPD1基因的表达被消除。编码甘油醛-3-磷酸脱氢酶的基因，诸如GPD1基因的表达可通过本领域普通技术人员已知的任何手段，诸如通过将核酸片段插入到GPD1基因座中或围绕GPD1基因座的调控区中来消除。

在一些实施方案中，本文所述的GE酵母，诸如酿酒酵母是二倍体并且具有减少或消除的两个拷贝的GPD1基因的表达。在一些实施方案中，GE酵母是二倍体并且在两个拷贝的GPD1基因中都包含缺失和/或插入。在其它实施方案中，GE酵母具有减少或消除的一个拷贝的GPD1基因的表达。

在一些实施方案中，本文所述的工程改造的酵母，诸如酿酒酵母，具有减少或消除的GPP1和/或GPP2的表达，并且还具有减少或消除的GPD1和/或GPD2的表达。在某些实施方案中，本文所述的工程改造的酵母诸如酿酒酵母，具有减少或消除的两个拷贝的GPP1的表达并且还具有减少或消除的一个拷贝的GPD1的表达。

本文所述的工程改造的酵母重组地表达一种或多种编码甘油醛-3-磷酸脱氢酶(gapN；对应于E.C.1.2.1.9；也称为“NADP依赖性非磷酸化甘油醛-3-磷酸脱氢酶”)的核酸。GapN酶将D-甘油醛3-磷酸转化为3-磷酸-D-甘油酯(Rosenberg等，Biol Chem(1955)217:361-71)。

应当理解，编码gapN酶的重组核酸可以来自任何来源。重组表达编码gapN酶的核酸的工程改造的酵母可包含或可以不包含编码gapN酶的内源基因。

在一些实施方案中，重组表达编码gapN酶的核酸的工程改造的酵母不包含编码gapN酶的基因的内源拷贝。因此，在此类实施方案中，编码gapN酶的核酸源自不同于所述工程改造的酵母的物种或生物体。

在其它实施方案中，重组表达编码gapN酶的核酸的工程改造的酵母确实包含编码gapN酶的基因的内源拷贝。在一些此类实施方案中，编码gapN酶的基因的内源拷贝或该基因的调控区，诸如启动子，被工程改造以增加编码gapN酶的基因的表达。在其它此类实施方案中，将编码gapN酶的核酸引入到酵母中。在此类实施方案中，被引入到酵母中的编码gapN酶的核酸可源自与其在其中表达的工程改造的酵母相同的物种或生物体，或者可以源自与其在其中表达的工程改造的酵母不同的物种或生物体。

在一些实施方案中，编码gapN酶的重组核酸包含蜡样芽孢杆菌基因(例如，GAPN，对应于UniProt号Q2HQS1)。在一些实施方案中，编码GapN酶或其部分的重组核酸是密码子优化的。在一些实施方案中，编码gapN酶或其部分的重组核酸包含SEQ ID NO:110。

在一些实施方案中，编码gapN酶或其部分的重组核酸与SEQ ID NO:110的序列具有至少或约50％、至少或约60％、至少或约70％、至少或约75％、至少或约80％、至少或约81％、至少或约82％、至少或约83％、至少或约84％、至少或约85％、至少或约86％、至少或约87％、至少或约88％、至少或约89％、至少或约90％、至少或约91％、至少或约92％、至少或约93％、至少或约94％、至少或约95％、至少或约96％、至少或约97％、至少或约98％、至少或约99％、至少或约99.5％或至少或约99.9％的序列同一性。

在一些实施方案中，gapN蛋白包含SEQ ID NO：111。在一些实施方案中，gapN蛋白与SEQ ID NO:111的序列具有至少或约50％、至少或约60％、至少或约70％、至少或约75％、至少或约80％、至少或约81％、至少或约82％、至少或约83％、至少或约84％、至少或约85％、至少或约86％、至少或约87％、至少或约88％、至少或约89％、至少或约90％、至少或约91％、至少或约92％、至少或约93％、至少或约94％、至少或约95％、至少或约96％、至少或约97％、至少或约98％、至少或约99％、至少或约99.5％或至少或约99.9％的序列同一性。

本领域普通技术人员将理解GAPN基因可源自任何来源，并且可以使用常规方法进行工程改造，诸如以改善在宿主细胞中的表达。另外，本领域普通技术人员将理解可在宿主细胞中的任何合适的基因座处插入GAPN基因。

如本文中所述，在一个方面，GE酵母可包含多种遗传修饰，而在发酵期间中不表现出显著的发酵性能的变化和/或酵母细胞健康的变化。令人惊讶地发现，包含本文所述的海藻糖酶性状与表达葡糖淀粉酶的性状和/或甘油还原性状组合的GE酵母(即，与可比较的野生型酵母相比，产生更少和/或消耗更多甘油的GE酵母)可以达到比任何其他目前可用的产乙醇酵母菌株更高的乙醇滴度，而没有表现出与GE酵母性能相关的任何重大的负面影响。在一个方面，在GE酵母中包含本文所述的一种或多种遗传修饰不会负面影响发酵的性能特征。在一些实施方案中，未被显著影响的发酵性能特征包括但不限于：乙醇的平均生产速率；给定底物，例如给定的玉米醪上的最大乙醇滴度；用于GE酵母产生最大乙醇滴度的时间；以及对于GE酵母产生商业相关滴度(例如，至少110g/L、至少120g/L或至少130g/L)的时间。商业上相关的发酵时间，即可产生商业上相关的滴度同时使制造商获利的发酵周期时间可以根据特定的乙醇工厂而变化。然而，对于本公开的目的，商业上相关的发酵时间被认为是48小时或更短，40小时或更短或36小时或更短。与野生型菌株(诸如Ethanol Red^TM)相比，不能在48小时内达到商业上相关的乙醇滴度和/或在48小时内的任何时间都表现出降低的乙醇生产速率的酵母菌株被认为表现出“发酵罚分(fermentation penalty)”。在一些实施方案中，与商业上相关的野生型酵母菌株相比，本发明的GE酵母可表现出显著减少的发酵罚分和/或无统计学意义的发酵罚分。

在一些实施方案中，GE酵母包含编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸和减少的或消除的编码甘油-3-磷酸磷酸酶(E.C.3.1.3.21)的基因的表达，以及还有编码海藻糖酶的异源基因。在一些实施方案中，GE酵母包含编码甘油醛-3-磷酸脱氢酶(GAPN，E.C.1.2.1.9)的重组核酸以及还有编码海藻糖酶的异源基因。在一些实施方案中，GE酵母可包含编码海藻糖酶的异源基因和编码葡糖淀粉酶的异源基因。

在一些实施方案中，GE酵母可包含编码海藻糖酶的异源基因；编码葡糖淀粉酶的异源基因；以及编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸和减少或消除的编码甘油-3-磷酸磷酸酶(E.C.3.1.3.21)的基因的表达。在一些实施方案中，GE酵母可包含编码海藻糖酶的异源基因；编码葡糖淀粉酶的异源基因；以及编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸。令人惊讶地表明，包含表达海藻糖酶的性状、表达葡糖淀粉酶的性状和/或甘油还原性状(例如，表达GAPN的性状)的GE酵母可产生比其它酵母菌株显著更高量的乙醇，而不表现出通常与产乙醇酵母的遗传修饰相关的任何显著的负面性能特征。

在一些实施方案中，所述编码海藻糖酶的异源基因可以是编码与以下多肽序列中的任一个具有至少80％、85％、90％或95％的序列同一性的海藻糖酶(EC 3.2.1.28)多肽的异源基因：SEQ ID NO：1(乳酸克鲁维酵母)、SEQ ID NO：2(近平滑假丝酵母)、SEQ ID NO：3(光滑假丝酵母)或SEQ ID NO：87(稻瘟病菌)。在一些实施方案中，GE酵母中编码葡糖淀粉酶的异源基因编码与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性的葡糖淀粉酶多肽：SEQ ID NO:16(Sf GA)、SEQ ID NO:17(Ro GA)、SEQID NO:108(Rmic GA)或SEQ ID NO:109(德氏根霉(R.delemar)GA，即Rdel GA)。

GE酵母可包含本文所述的特定异源海藻糖酶基因和特定葡糖淀粉酶基因的任何组合。非限制性实例是包含来自稻瘟病菌的异源海藻糖酶基因和来自小孢根霉的异源GA基因的GE酵母；包含来自稻瘟病菌的异源海藻糖酶基因和来自扣囊复膜酵母菌的异源GA基因的GE酵母；包含来自稻瘟病菌的异源海藻糖酶基因和来自戴尔根霉的异源GA基因的GE酵母；包含来自稻瘟病菌的异源海藻糖酶基因和来自米根霉的异源GA基因的GE酵母；包含来自光滑假丝酵母的异源海藻糖酶基因和来自小孢根霉的异源GA基因的GE酵母；包含来自光滑假丝酵母的异源海藻糖基因和来自扣囊复膜酵母菌的异源GA基因的GE酵母；包含来自近平滑假丝酵母的异源海藻糖酶基因和来自小孢根霉的异源GA基因的GE酵母；包含来自近平滑假丝酵母的异源海藻糖基因和来自扣囊复膜酵母菌的异源GA基因的GE酵母以胶包含来自近平滑假丝酵母的异源海藻糖基因和来自戴尔根霉的异源GA基因的GE酵母。另外，GE酵母的任何上述实例(和本文提供的GE酵母的任何其它实例)也可包括如本文所述的甘油还原(GR)的形式。应当理解，在本文所述的任何实施方案中，当本公开提及包含来自某些物种的异源基因的GE酵母时，GE酵母中的此类基因将编码与该基因相关的多肽(在本申请的序列表中提供了此类多肽，包括具有和不具有天然前导序列或具有非天然前导序列的此类多肽的形式的非限制性实例)。

应当理解，GE酵母可包含本文描述的性状的任何组合。例如，GE酵母可表达任何海藻糖酶，也可表达任何葡糖淀粉酶，并且还可包含一种或多种甘油还原性状的任何形式。除了这三个性状(或这些性状中的两个的任何组合)以外，GE酵母还可表达任何异麦芽糖酶和/或可包含消耗乳酸的性状。令人惊奇地发现，GE酵母能够包含本文所述的每种性状的形式而不会表现出显著的发酵罚分。

发酵方法

在一个方面，本发明涉及发酵方法。在一个方面，发酵方法可以是使用本文描述的经遗传修饰的酵母的实施方案来产生发酵产物的任何方法。在一些实施方案中，发酵产物是乙醇。在一些实施方案中，发酵产物可以是除乙醇外的发酵产物，例如但不限于正丙醇、异丙醇、正丁醇、异丁醇、丁二烯或异戊二烯。

示例性的发酵方法可包括以下步骤：提供包含碳源的发酵培养基，将酵母添加到发酵培养基中，用酵母发酵该培养基以产生生物产物，以及收获该生物产物。在一个方面，培养基中的碳源可包括淀粉、糖、有机酸或其混合物。在一些实施方案中，糖可包括海藻糖。在一些实施方案中，培养基可包含乳酸盐。

在一个方面，如本领域技术人员将理解的，培养基的组成在发酵期间可以变化。例如，葡萄糖或另一种己糖可在发酵期间通过酶活性由寡聚物产生，然后被消耗掉。因此，在一些实施方案中，如果葡萄糖被酵母消耗比其从葡萄糖寡聚物产生更快，则葡萄糖含量在发酵的某些点可能非常低，甚至无法检测。在一些实施方案中，例如分批补料发酵，可以向所述培养基连续或半连续地补充进料流，诸如蔬菜加工进料流。

因此，在一个方面，用于本文描述的方法的发酵培养基的各种组分的浓度可以是平均浓度。组分的平均浓度可通过本领域中已知的方法来计算，例如通过获取发酵开始时的发酵培养基中组分的浓度和发酵结束时的发酵培养基中相同组分的浓度的平均值来计算。平均值的这种计算还可考虑发酵方法过程中任何输入和/或输出流中组分的浓度。另外，对于连续发酵方法，组分的平均浓度可以指任何单个容器中的平均浓度，或者其可以指整个过程中的平均浓度，即，考虑到过程的所有进料流和所有输出流。

在一些实施方案中，至少5％、10％、15％、20％、25％、30％、35％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或99％的存在于发酵培养基中和/或在发酵期间由酵母产生的海藻糖在发酵结束时被消耗掉。

在一些实施方案中，发酵结束时发酵培养基中海藻糖的量在0至10g/L、0至5g/L、0至4g/L、0至3g/L、0至1g/L、0至0.5g/L、0至0.1g/L、0.001至3g/L、0.001至1g/L或0.001至0.1g/L的范围内。在一些实施方案中，发酵结束时发酵培养基中海藻糖的量在0.5至5g/L、1至5g/L、0.5至4g/L、0.5至3g/L、0.5至2g/L或0.1至2g/L的范围内。在一些实施方案中，发酵结束时发酵培养基中总海藻糖的量小于10g/L、7.5g/L、5g/L、4g/L、3g/L、2g/L、1g/L、0.5g/L或0.1g/L。

在一些实施方案中，至少0.1g/L、0.5g/L、1g/L、2g/L、3g/L、4g/L、5g/L、6g/L、7g/L、8g/L、9g/L或10g/L海藻糖在此过程中被转化成葡萄糖。与海藻糖转化成葡萄糖有相关的上述参数涉及不包括外源海藻糖酶的过程，即海藻糖的转化是由GE酵母表达的海藻糖酶进行的。在一些实施方案中，在本文描述的任何方法中海藻糖向葡萄糖的转化还与大量乙醇产生相关，例如至少40g/L、45g/L、50g/L、55g/L、60g/L、65g/L、70g/L、75g/L、80g/L、85g/L、90g/L、95g/L、100g/L、105g/L、110g/L、115g/L、120g/L、125g/L、130g/L或135g/L。另外，在一个方面，本发明的方法可用于以商业上显著的速率和/或滴度生产乙醇。在一些实施方案中，乙醇的产生速率可以是1至6g L^-1h^-1、1至5.5g L^-1h^-1或1至5g L^-1h^-1。

在一些实施方案中，可以将外源酶添加到该过程中。例如，在一些实施方案中，可以将外源海藻糖酶、葡糖淀粉酶和/或异麦芽糖酶添加到发酵方法中。

在目前的发酵方法中，海藻糖的来源主要来自酵母本身。最初的发酵液中可能存在少量海藻糖，但是，大多数可能是由酵母产生的。由酵母产生的海藻糖的量与对酵母的应激水平相关。因此，产生大量乙醇的过程，诸如本发明的过程，可对酵母造成乙醇相关的应激，从而增加存在于发酵液中的海藻糖的量。诱导酵母合成海藻糖的应激也可由其它因素诸如热或升高的盐浓度引起。在一些实施方案中，使用纤维素制品作为底物进行的发酵方法与大量的海藻糖形成相关。这些纤维素乙醇过程可诱导更多对酵母的应激。因此，本文所述的酵母对于使用纤维素材料作为主要发酵底物的发酵方法可以是特别有利的，或者除了葡萄糖或葡萄糖低聚物以外还具有大量的纤维素材料。

分批发酵方法

在一个方面，本发明的方法可以是分批发酵方法。在一些实施方案中，本发明的分批方法是干磨或干磨乙醇生产方法。分批发酵方法，包括干磨乙醇方法是本领域公知的。

示例性分批发酵方法包括以下步骤：提供含有碳源诸如碳水化合物的发酵培养基，并使用本文所述类型的遗传工程改造的酵母使该培养基发酵。在一些实施方案中，酵母包含异源海藻糖酶基因。在一些实施方案中，在发酵开始时，培养基包含浓度为至少0.5g/L、1g/L、2g/L或3g/L的葡萄糖或葡萄糖寡聚物。

连续发酵方法

在一个方面，本发明的方法可以是连续的或半连续的发酵方法。在一些实施方案中，本发明的连续方法是湿法玉米研磨乙醇生产方法。包括湿磨乙醇方法在内的连续发酵方法是本领域中公知的。在一些实施方案中，具有连续操作模式的发酵方法包括多个串联操作的发酵罐，其中在第一发酵罐中供应淀粉水解产物，将淀粉水解产物供给第二发酵罐，依此类推，直到淀粉水解产物转化为乙醇。在一些实施方案中，可使用1至10个或2至7个发酵罐进行连续操作。在一些实施方案中，可在单个容器中进行连续发酵方法，其中可按照连续或半连续的时间表添加原料并且可以去除含有产物的发酵液。

用于生产乙醇的示例性连续发酵方法包括以下步骤：提供包含葡萄糖或葡萄糖寡聚物的初始发酵培养基，用经遗传修饰的酵母使发酵培养基发酵，以及从发酵培养基中除去至少一种包含乙醇的输出流。

在一些实施方案中，将初始发酵培养基添加至预发酵罐或生长发酵罐容器中，在其中添加经遗传修饰的酵母并使其生长直至获得所需的生物质。在一些实施方案中，将预发酵罐中的过程条件设置为有利于细胞生长而非发酵产物形成。在一些实施方案中，然后可以将预发酵罐容器中的内容物转移至第二发酵罐容器中。在第二个发酵罐容器中，将过程条件设置为有利于发酵产物的形成而非细胞生长。在一些实施方案中，以单部分或以连续或半连续的方式将另外的发酵培养基添加到第二发酵罐容器中。在一些实施方案中，添加至第二发酵罐容器的另外的发酵培养基含有乳酸和/或其它碳源。上面提及的第二发酵罐也可以称为“培育箱(propagator)”。在一些实施例中，第二发酵罐容器的内容物可被转移到第三发酵罐容器。第三发酵罐容器的过程条件可以与第二发酵罐容器相同或不同。在一些实施方案中，可将第三发酵罐容器的内容物转移至一个或多个另外的发酵罐容器，如连续发酵方法领域的技术人员所理解的。在一些实施方案中，从最终发酵罐容器的内容物中分离出生物产物，例如乙醇。

在一些实施方案中，预发酵罐容器中发酵培养基的平均葡萄糖浓度在10至20g/L的范围内。在一些实施方案中，第二发酵罐容器中发酵培养基的平均葡萄糖浓度在30至40g/L的范围内。在一些实施方案中，第三发酵罐容器或任何另外的发酵罐容器中发酵培养基的平均葡萄糖浓度在30至40g/L的范围内。在一些实施方案中，最终发酵罐容器中发酵培养基的平均葡萄糖浓度在0至5g/L的范围内。在一些实施方案中，预发酵罐容器、培育箱容器中或任何发酵容器中的发酵培养基的平均葡萄糖浓度在0-5g/L、2-5g/L、1-10g/L、5-10g/L、5-15g/L、5-20g/L、10-20g/L、15-25g/L、20-30g/L、25-35g/L、30-40g/L或35-45g/L的范围内。在一些实施方案中，将预发酵罐容器、培育箱容器中或任何发酵容器中的发酵物的平均葡萄糖浓度维持在大于或等于与酵母中的葡萄糖阻抑相关的葡萄糖浓度的范围内。在一些实施方案中，酵母中与葡萄糖阻抑相关的葡萄糖浓度在2至5g/L的范围内。因此，在此类实施方案中，可将预发酵罐容器中或任何发酵容器中的发酵培养基的平均葡萄糖浓度维持在大于或等于2g/L、3g/L、4g/L或5g/L的水平。

可以在连续发酵方法中调节和/或维持其它发酵条件，包括但不限于：温度、pH、体积或比耗氧速率(OUR)或任何碳源或任何发酵培养基营养物的浓度。在一些实施方案中，预发酵罐容器、培育箱容器中或任何其它发酵容器中的温度可在20-45℃、20-40℃、20-30℃、25-35℃或30-40℃的范围内。在一些实施方案中，预发酵罐、培育箱容器或中任何其它发酵容器中的pH可在2至7、3至6、4.5至5.5或3.5至4.5的范围内。

在一些实施方案中，预发酵罐容器中的细胞密度在3至10g/L或5至10g/L的范围内。在一些实施方案中，培育箱容器中的细胞密度在10至50g/L的范围内。

在一个方面，所述方法使用本文所述的酵母，其在发酵方法期间消耗通过使用海藻糖酶的催化从海藻糖产生的葡萄糖。在一些实施方案中，所有输出流的总和中的总海藻糖含量小于添加到发酵方法中或在发酵方法中产生的海藻糖酶的90％。在一些实施方案中，发酵方法的输出中的总海藻糖含量小于发酵方法期间产生的海藻糖的99％、95％、85％、80％、75％、70％、65％、60％、55％、50％、45％、40％、35％、30％、25％、20％、15％或10％。在一些实施方案中，发酵方法的所有输出流的总和中的海藻糖含量小于10g/L、9g/L、8g/L、7g/L、6g/L、5g/L、4g/L、3g/L、2g/L、1g/L、0.5g/L或0.1g/L。

本文所述的连续发酵方法可以以商业上显着的速率产生乙醇或另一种生物产物。在一些实施方案中，所述方法可以以至少0.5、1.0、1.5、2.0、2.5、3.0或3.2g L^-1h^-1的速率产生生物产物。

实验实施例

通过参考以下实验实施例进一步详细地描述本发明。除非另外指明，否则提供这些实施例仅仅是为了说明的目的，并非旨在为限制性的。因此，决不应将本发明解释为限于以下实施例，相反，应解释为涵盖由于本文提供的教导而变得显而易见的任何及所有变型。本公开总体上涉及产生乙醇的酿酒酵母的实施方案。然而，本公开不限于此类酵母和发酵产物。针对其它酵母种和/或生物产物的实施方案旨在包括在本公开的教导和发明内。

实施例1：表达海藻糖酶的经遗传修饰的酵母菌株

在本实施例中，酿酒酵母菌株被转化为表达来自乳酸克鲁维酵母、近光滑假丝酵母和光滑假丝酵母的酸性海藻糖酶。使用TDH3启动子表达酸性海藻糖酶。每种海藻糖酶的形式用该基因的天然信号肽合成，并且每种海藻糖酶的第二形式用来自酿酒酵母的MFα2信号肽合成。酿酒酵母菌株也被转化为表达使用SAM2启动子表达的来自稻瘟病菌的海藻糖酶。所选菌株也被转化为包含葡糖淀粉酶表达和/或与甘油还原相关的基因。转化的菌株用于产生乙醇的发酵中。转化的菌株消耗发酵中形成的海藻糖，并且与不表达异源海藻糖酶的菌株相比，转化的菌株经显示产生更高量的乙醇。

菌株构建

菌株1

2016年3月25日提交的国际专利申请公开号WO 2016/160584中描述的菌株1-3是酿酒酵母菌株(菌株14883，Ethanol Red^TM酿酒酵母的形式)，其中两个ScURA3基因拷贝均被缺失。对于本公开的目的，菌株1-3被称为菌株1。

菌株2

用SEQ ID NO：6转化菌株1。SEQ ID NO：6包含：i)包含ScTDH3启动子的空表达盒；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQ ID NO：6至选菌落中的正确整合。将PCR验证的分离株命名为菌株2。

菌株3

用SEQ ID NO：7转化菌株1。SEQ ID NO：7包含：i)具有MFα2分泌信号的来自乳酸克鲁维酵母的海藻糖酶的表达盒，其编码由TDH3启动子表达的氨基酸序列SEQ ID NO：8；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQ ID NO:7至所选菌落中的正确整合。将PCR验证的分离株命名为菌株3。

菌株4

用SEQ ID NO：9转化菌株1。SEQ ID NO：9包含：i)来自乳酸克鲁维酵母的海藻糖酶的表达盒，其编码由TDH3启动子表达的氨基酸序列SEQ ID NO：1；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQID NO:9至所选菌落中的正确整合。将PCR验证的分离株命名为菌株4。

菌株5

用SEQ ID NO：10转化菌株1。SEQ ID NO：10包含：i)具有MFα2分泌信号的来自近平滑假丝酵母的海藻糖酶的表达盒，其编码由TDH3启动子表达的氨基酸序列SEQ ID NO：11；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQ ID NO:10至所选菌落中的正确整合。将PCR验证的分离株命名为菌株5。

菌株6

用SEQ ID NO：12转化菌株1。SEQ ID NO：12包含：i)来自近平滑假丝酵母的海藻糖酶的表达盒，其编码由TDH3启动子表达的氨基酸序列SEQ ID NO：2；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQID NO:12至所选菌落中的正确整合。将PCR验证的分离株命名为菌株6。

菌株7

用SEQ ID NO：13转化菌株1。SEQ ID NO：13包含：i)具有MFα2分泌信号的来自光滑假丝酵母的海藻糖酶的表达盒，其编码由TDH3启动子表达的氨基酸序列SEQ ID NO：14；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQ ID NO:13至所选菌落中的正确整合。将PCR验证的分离株命名为菌株7。

菌株8

用SEQ ID NO：15转化菌株1。SEQ ID NO：15包含：i)来自光滑假丝酵母的海藻糖酶的表达盒，其编码由TDH3启动子表达的氨基酸序列SEQ ID NO：3；ii)ScURA3表达盒；iii)用于稳定复制的酿酒酵母CEN6着丝粒；和iv)β-内酰胺酶表达盒。在ScD-Uracil板上选择转化体。将所得转化体在ScD-Uracil板上划线进行单菌落分离。选择单菌落。通过PCR验证SEQID NO:15至所选菌落中的正确整合。将PCR验证的分离株命名为菌株8。

菌株9

用SEQ ID NO:93和SEQ ID NO:94共转化菌株1。SEQ ID NO:93含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)URA3启动子以及URA3基因的一部分。SEQ ID NO：94包含以下元件：i)URA3基因的一部分和终止子；以及ii)与天然FCY1基因的3'区域同源的DNA。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株9。

菌株10

用SEQ ID NO:96和SEQ ID NO:97共转化菌株9。SEQ ID NO:96含有以下元件：i)与天然FCY1基因的5’区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)TEF1启动子以及构巢曲霉(Aspergillus nidulans)amdS基因的一部分。SEQ ID NO：97包含以下元件：i)构巢曲霉乙酰胺酶(amdS)基因的一部分和TEF1终止子；以及ii)与天然FCY1基因的3'区域同源的DNA。在YNB+乙酰胺板上选择转化体。将所得转化体在YNB+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株10。

菌株11

用SEQ ID NO：98转化菌株10。在含有3.5g/L对氟苯丙氨酸和1g/L L-酪氨酸(ScD-PFP)的合成完全培养基上选择转化体。将所得转化体在ScD-PFP上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株11。

菌株12

用SEQ ID NO：99转化菌株11。在ScD-ura上选择转化体。将所得转化体在ScD-ura上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株12。

菌株13

用SEQ ID NO:100和SEQ ID NO:101以及SEQ ID NO:102和SEQ ID NO:103共转化菌株11。在YNB+乙酰胺板上选择转化体。将所得转化体在YNB+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过测序确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株13。

菌株14

用SEQ ID NO：98转化菌株13。在含有3.5g/L对氟苯丙氨酸和1g/L L-酪氨酸(ScD-PFP)的合成完全培养基上选择转化体。将所得转化体在ScD-PFP上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株14。

菌株15

用SEQ ID NO：99转化菌株14。在ScD-ura上选择转化体。将所得转化体在ScD-ura上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株15。

菌株16

用SEQ ID NO:93和SEQ ID NO:104共转化菌株11。SEQ ID NO:93含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQ ID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)URA3启动子以及URA3基因的一部分。SEQ ID NO：104包含以下元件：i)URA3基因的一部分和终止子；和ii)具有MFα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(SEQ ID NO：92)的表达盒，其处于SAM2启动子(SEQ ID NO：112)和GAL10终止子的控制下；以及iii)与天然FCY1基因的3'区同源的DNA。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株16。

菌株17

用SEQ ID NO:96和SEQ ID NO:105共转化菌株16。SEQ ID NO:96含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQ ID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)TEF1启动子以及构巢曲霉amdS基因的一部分。SEQ ID NO：105包含以下元件：i)构巢曲霉乙酰胺酶(amdS)基因的一部分和ADH1终止子；和ii)具有MFα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(SEQ ID NO：92)的表达盒，其处于SAM2启动子(SEQ ID NO：112)和GAL10终止子的控制下；以及iii)与天然FCY1基因的3'区同源的DNA。在YNB+乙酰胺板上选择转化体。将所得转化体在YNB+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株17。

菌株18

用SEQ ID NO：98转化菌株17。在含有3.5g/L对氟苯丙氨酸和1g/L L-酪氨酸(ScD-PFP)的合成完全培养基上选择转化体。将所得转化体在ScD-PFP上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株18。

菌株19

用SEQ ID NO：99转化菌株18。在ScD-ura上选择转化体。将所得转化体在ScD-ura上划线进行单菌落分离。选择单菌落。将PCR验证的分离株命名为菌株19。

菌株20

用SEQ ID NO:93和SEQ ID NO:106共转化菌株11。SEQ ID NO:93含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQ ID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)URA3启动子以及URA3基因的一部分。SEQ ID NO：106含有以下元件：i)URA3基因的一部分和终止子；和ii)具有MFα2分泌信号的光滑假丝酵母海藻糖酶的密码子优化的变体(SEQ ID NO：14)的表达盒，其处于SAM2启动子(SEQ ID NO：112)和GAL10终止子的控制下；以及iii)与天然FCY1基因的3'区同源的DNA。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株20。

菌株21

用SEQ ID NO:96和SEQ ID NO:107共转化菌株20。SEQ ID NO:96含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQ ID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)TEF1启动子以及构巢曲霉amdS基因的一部分。SEQ ID NO：107含有以下元件：i)构巢曲霉乙酰胺酶(amdS)基因的一部分和ADH1终止子；和ii)具有MFα2分泌信号的光滑假丝酵母海藻糖酶的密码子优化的变体(SEQ ID NO：14)的表达盒，其处于SAM2启动子(SEQ ID NO：112)和GAL10终止子的控制下；以及iii)与天然FCY1基因的3'区同源的DNA。在YNB+乙酰胺板上选择转化体。将所得转化体在YNB+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株21。

菌株22

用SEQ ID NO:93和SEQ ID NO:104共转化菌株14。SEQ ID NO:93含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQ ID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)URA3启动子以及URA3基因的一部分。SEQ ID NO：104含有以下元件：i)URA3基因的一部分和终止子；和ii)具有MFα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(SEQ ID NO：92)的表达盒，其处于SAM2启动子(SEQ ID NO：112)和GAL10终止子的控制下；以及iii)与天然FCY1基因的3'区同源的DNA。在ScD-Ura上选择转化体。将所得转化体在ScD-Ura上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株22。

菌株23

用SEQ ID NO:96和SEQ ID NO:105共转化菌株22。SEQ ID NO:96含有以下元件：i)与天然FCY1基因的5'区同源的DNA；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(SEQ ID NO:95)的表达盒，其处于TDH3启动子和CYC1终止子的控制下；以及iii)TEF1启动子以及构巢曲霉amdS基因的一部分。SEQ ID NO：105含有以下元件：i)构巢曲霉乙酰胺酶(amdS)基因的一部分和ADH1终止子；和ii)具有MFα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(SEQ ID NO：92)的表达盒，其处于SAM2启动子(SEQ ID NO：112)和GAL10终止子的控制下；以及iii)与天然FCY1基因的3'区同源的DNA。在YNB+乙酰胺板上选择转化体。将所得转化体在YNB+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过PCR确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株23。

表1.菌株的描述

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实施例2：在摇瓶测定中表征菌株

摇瓶法

将菌株2-8划线至ScD-Ura板并且在30℃下孵育直至单菌落可见(1-2天)。将来自ScD-Ura板的细胞刮入无菌摇瓶培养基中，并且测量光密度(OD600)。使用Genesys20型分光光度计(Thermo Scientific)在600nm波长和1cm路径长度下测量光密度。用细胞浆液接种摇瓶以达到0.1-0.3的初始OD600。在即将接种之前，将50mL摇瓶培养基添加到250mL无挡板摇瓶(Corning 4995-250)中，该摇瓶装配有含有透气密封件(corning 1395-45LTMC)的螺纹盖。摇瓶培养基由725g部分水解的玉米淀粉、150g过滤后的轻浸渍水、50g水、25g葡萄糖和1g尿素组成。将每种菌株的一式二份烧瓶在30℃和80％湿度下在定轨振荡器中以100rpm振荡孵育48小时。取样并通过高效液相色谱(HPLC)分析发酵期间发酵液中的乙醇和海藻糖的浓度。

结果

在每个图中，对照菌株为菌株2；含有来自乳酸克鲁维酵母的异源海藻糖酶的菌株是菌株3和4；含有来自近平滑假丝酵母的异源海藻糖酶的菌株是菌株5和6；以及含有来自光滑假丝酵母的异源海藻糖酶的菌株是菌株7和8。

图1显示在不添加海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株消耗显著更多的在发酵期间产生的海藻糖。

图2和图3显示在不添加海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株产生显著更多的乙醇。

图4显示，在开始前添加10g/L海藻糖的发酵中，含有来自乳酸克鲁维酵母(3&4)、近平滑假丝酵母(5&6)和光滑假丝酵母(7&8)的异源海藻糖酶的菌株比对照菌株(2)消耗显著更多的海藻糖。同样，与使用相应基因的天然信号肽的相同基因相比，利用MFα2信号肽的所有3种异源海藻糖酶均以比利用各自基因的天然信号肽的同一基因更快的速率水解海藻糖。含有近平滑假丝酵母和光滑假丝酵母异源海藻糖酶的菌株全部均达到约1g/L的最终海藻糖滴度，导致如图5和图6中显示的相似的48小时乙醇滴度。

图5和图6显示，在开始前添加10g/L海藻糖的发酵中，含有来自乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母的异源海藻糖酶的菌株比对照菌株产生显著更多的乙醇。含有近平滑假丝酵母和光滑假丝酵母异源海藻糖酶的菌株比含有乳酸克鲁维酵母海藻糖酶的菌株产生显著更多的乙醇。

图7显示，在即将在40小时取样之前向发酵中添加10g/L海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株消耗显著更多的海藻糖。数据显示，海藻糖酶在发酵后期仍具有显著活性。

图8和图9显示，在即将在40小时取样之前向发酵中添加10g/L海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株产生显著更多的乙醇。

实施例3：在33.3℃下在32％的DS玉米醪中菌株的表征

将菌株12、15、19、21、23和24划线至YPD板并且在30℃下孵育直至单菌落可见(1-2天)。将来自YPD板的细胞刮入pH 7.0的磷酸盐缓冲液中，并测量光密度(OD₆₀₀)。使用Genesys20型分光光度计(Thermo Scientific)在600nm波长和1cm路径长度下测量光密度。用细胞浆液接种摇瓶以达到为0.1的初始OD₆₀₀。在即将接种之前，将以下材料添加到每个烧瓶中：将50克液化玉米醪添加到250mL带挡板的摇瓶(该摇瓶用气锁(air-lock)密封)中，所述摇瓶含有4ml无菌芥花油、190ul 500g/L过滤灭菌的尿素和2.5ul 100mg/ml过滤灭菌的氨苄青霉素。将0.284AGU/g DS(70μl的1:2稀释液)的葡糖淀粉酶(来自黑曲霉的淀粉葡糖苷酶，Sigma)添加到含有对照菌株24的烧瓶中，并将0.114AGU/g DS(28μl的1:2稀释液)的葡糖淀粉酶(来自黑曲霉的淀粉葡糖苷酶，Sigma)添加到剩余的烧瓶中。来自黑曲霉的淀粉葡萄糖苷酶(Sigma(目录号A7095))据估计具有约260AGU/ml的酶水溶液。将每种菌株的一式二份烧瓶在33.3℃下在定轨振荡器中以100rpm振荡孵育约48小时。在48小时时，获取1ml样品，并通过高效液相色谱(HPLC)分析其发酵液中的乙醇和海藻糖的浓度。

图10和图11显示了玉米醪发酵中所选菌株的结果。含有异源海藻糖酶的菌株(菌株19、21和23)以及还有小孢根霉GA(Rmic GA)与野生型菌株(菌株24)或具有Rmic GA而无异源海藻糖酶的菌株(菌株12)相比，在发酵结束时显示出显著更高的乙醇产量和显著更低的存在的海藻糖。菌株15(其包含Rmic GA和甘油还原性状两者，但不包含异源海藻糖酶)显示出比一些包含异源海藻糖酶的菌株更高的乙醇滴度。然而，具有与菌株15相同的性状而且还包含异源海藻糖酶的相应菌株(菌株23)，显示出在所有测试菌株中最高的乙醇滴度。

实施方案

提供以下实施方案作为实施方案的非限制性实例。本申请不仅仅限于这些实施方案。

实施方案A.一种经遗传修饰的酵母，其包含编码海藻糖酶(EC3.2.1.28)多肽的异源基因，

其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇。

B.如实施方案A的酵母，其中所述海藻糖酶多肽是酸性海藻糖酶。

C.如实施方案A-B中任一项的酵母，其中编码海藻糖酶多肽的基因源自选自由以下组成的组的生物体：稻瘟病菌、乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母。

D.如实施方案A-C中任一项的酵母，其中由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQ ID NO：1、SEQ ID NO：2、SEQ ID NO：3或SEQ ID NO：87。

E.如实施方案A-D中任一项的酵母，其中由酵母编码的海藻糖酶多肽包含与SEQID NO：83具有至少70％、80％、90％或95％的序列同一性的序列。

F.如实施方案A-E中任一项的酵母，其中由酵母编码的海藻糖酶多肽包含与SEQID NO：84和/或SEQ ID NO：85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列。

G.如实施方案A-F中任一项的酵母，其中所述酵母是经遗传修饰的酿酒酵母。

H.如实施方案A-G中任一项的酵母，其中由所述酵母编码的海藻糖酶包含MFα2信号序列。

I.如实施方案H的酵母，其中所述MFα2信号序列是SEQ IDNO：4。

J.如实施方案H的酵母，其中所述MFα2信号序列与SEQ ID NO：4具有至少84％、89％或94％的序列同一性。

K.如实施方案A-J中任一项的酵母，其中由酵母编码的海藻糖酶与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：SEQ ID NO：8、SEQ ID NO：11、SEQ ID NO：14或SEQ ID NO：92。

L.如实施方案A-K中任一项的酵母，其还包含编码葡糖淀粉酶(EC 3.2.1.3)多肽的异源基因。

M.如实施方案L的酵母，其中编码葡糖淀粉酶多肽的异源基因是源自选自由以下组成的组的物种的葡糖淀粉酶基因：树脂枝胞霉、黑曲霉、泡盛曲霉、米曲霉、白曲霉、白宇佐美曲霉、食腺嘌呤芽生葡萄孢酵母、白假丝酵母、米根霉、粟酒裂殖酵母、扣囊复膜酵母菌布鲁塞尔酒香酵母和产朊假丝酵母。

N.如实施方案L的酵母，其中由酵母编码的葡糖淀粉酶多肽与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性：SEQ ID NO：16、SEQID NO：17、SEQ ID NO：18或SEQ ID NO：19。

O.如实施方案A-N中任一项的酵母，其还包含编码异麦芽糖酶(EC 3.2.1.10)多肽的异源基因。

P.如实施方案A-O中任一项的酵母，其中所述酵母编码与SEQ ID NO：20的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白多肽。

Q.如实施方案A-O中任一项的酵母，其中所述酵母编码与SEQ ID NO：21的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白多肽。

R.如实施方案A-Q中任一项的酵母，其还包含编码细胞色素b2(CYB2)(EC1.1.2.3)多肽的异源基因。

S.如实施方案R的酵母，其中所述CYB2多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32或SEQ ID NO:33。

T.如实施方案R的酵母，其中所述CYB2多肽在SEQ ID NO:27、SEQ ID NO:28、SEQID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32或SEQ ID NO:33中指定的位置处包含以下残基中的一个或多个：Lys349、Tyr143、Tyr254和His373。

U.如实施方案A-T中任一项的酵母，其还包含编码D-乳酸脱氢酶(DLD)(EC1.1.2.4)多肽的异源基因。

V.如实施方案U的酵母，其中所述编码的DLD多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40或SEQ ID NO:41。

W.如实施方案A-V中任一项的酵母，其还包含编单羧酸/单羧酸盐转运蛋白多肽的异源基因。

X.如实施方案W的酵母，其中由酵母编码的单羧酸/单羧酸盐转运蛋白多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：SEQ ID NO:22、SEQ IDNO:23、SEQID NO:24、SEQ ID NO:25或SEQ ID NO:26。

Y.如实施方案A-X中任一项的酵母，其中所述酵母以足以在乙醇滴度为至少为75g/L时，将发酵液中的海藻糖含量降低至小于0.5g/L、1g/L、2g/L、3g/L、4g/L、5g/L、6g/L、7g/L、8g/L、9g/L或10g/L的量分泌海藻糖酶。

Z.如实施方案A-Y中任一项的酵母，其中所述酵母能够在细胞外分泌海藻糖酶。

AA.如实施方案A-Z中任一项的酵母，其中由酵母编码的海藻糖酶多肽包含与SEQID NO：86具有至少76％、至少84％、至少92％或100％的序列同一性的序列。

BB.如实施方案A-AA中任一项的酵母，其中所述酵母能够以80g/L、90g/L、100g/L、110g/L、120g/L、125g/L、130g/L、135g/L或更高的滴度产生乙醇。

CC.一种用于生产乙醇的方法，所述方法包括：

使用经遗传修饰的酵母使培养基发酵，其中所述酵母包含异源海藻糖酶基因，其中发酵结束时的乙醇滴度至少为90g/L。

DD.如实施方案CC的方法，其中发酵温度在25至45℃、25至40℃、25至35℃、30至40℃或28至38℃的范围内。

EE.如实施方案CC-DD中任一项的方法，其中发酵结束时的乙醇滴度为至少80g/升、90g/升、100g/升、110g/升、120g/升、130g/升、135g/升、140g/升、145g/升、150g/升、155g/升或160g/升。

FF.如实施方案CC-EE中任一项的方法，其中所述酵母是如实施方案A-BB中任一项的酵母。

GG.如实施方案A-FF中任一项的酵母或方法，其中所述酵母还包含编码葡糖淀粉酶(EC 3.2.1.3)多肽的异源基因。

HH.如实施方案GG的酵母或方法，其中由酵母编码的葡糖淀粉酶与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性：SEQ ID NO:16(Sf GA)、SEQ ID NO:17(Ro GA)、SEQ ID NO:108(Rmic GA)或SEQ ID NO:109(Rdel GA)。

II.如实施方案A-HH中任一项的酵母或方法，其中所述酵母包含编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸；以及减少的或消除的编码甘油-3-磷酸磷酸酶(E.C.3.1.3.21)的基因的表达。

JJ.如实施方案A-HH中任一项的酵母或方法，其中所述酵母包含编码甘油醛-3-磷酸脱氢酶(GAPN，E.C.1.2.1.9)的重组核酸。

KK.如实施方案A-JJ中任一项的酵母或方法，其中所述编码甘油醛-3-磷酸脱氢酶(E.C.1.2.1.9)的重组核酸编码与SEQ ID NO：111(蜡样芽孢杆菌GAPN)具有至少80％、85％、90％或95％的序列同一性的多肽。

本文引用的每个专利、专利申请或出版物的公开内容由此通过引用整体并入。虽然已参照具体的实施方案公开了本发明，但本发明的其它实施方案和变型可由本领域的其他技术人员在不脱离本发明的精神和范围的情况下设计。所附权利要求旨在被解释为包括所有此类实施方案和变型。

序列表

<110> 嘉吉公司(Cargill, Incorporated)

彼得·豪尔特(Jauert, Peter)

格雷戈里·庞特(Poynter, Gregory)

布来恩·拉什(Rush, Brian)

<120> 经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法

<130> N00525-US-PSP

<150> 62/501,288

<151> 2017-05-04

<160> 112

<170> PatentIn 3.5版

<210> 1

<211> 1147

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 1

Met Leu Ile Val Pro Phe Ile Leu Phe Ser Val Ala Ile Leu Ala Pro

1 5 10 15

Ile Tyr Phe Tyr Leu Thr Lys Pro Leu Pro Leu Gln His Thr His Phe

20 25 30

Gly Ser Phe Val Cys Ala Glu Asp Thr Leu Phe Cys Pro Glu Ser Gln

35 40 45

Arg Lys Ala Ser Glu Lys Met Tyr Ser Leu Leu Lys Asp His Glu Asn

50 55 60

Thr Phe Tyr Asp Glu Glu Gln Gln Ile Leu Gly Asn Leu Leu Leu Ser

65 70 75 80

Glu Asn Thr Phe Ser Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly

85 90 95

Ser Arg Ile Pro Asn Leu Gly Phe Gly Tyr Ala Leu Asp Thr Ile Asn

100 105 110

Val Trp Val Asn Asp Ser Ser Ile Pro Gly Ala Leu Asp Asn Gly Trp

115 120 125

Pro Leu Arg Asn Gln Arg Phe Ala Gly Ala Phe Ile Ser Asp Phe Tyr

130 135 140

Cys Leu Gln Glu Lys Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp Asp

145 150 155 160

Asp Gly Tyr Ser Thr Val Ile Ser Thr Ile Pro Gln Trp Thr Asp Leu

165 170 175

Ser Ile Leu Lys His Thr Thr Thr Gly Gln Ile Glu Tyr Ile Asn Pro

180 185 190

Thr Asp Val Lys Leu Asp Lys Ile Thr Asn Tyr Met Gln Asn Leu Ser

195 200 205

Leu Gln Asp Gly Ile Val Thr Thr Thr Phe Val Tyr Asp Lys Gln Leu

210 215 220

Leu Val Thr Thr Lys Val Val Ala His Arg Lys Ile Tyr Pro Leu Gly

225 230 235 240

Val Val Thr Leu Glu Leu Ser Leu Phe Asp Asn Asn Ser Asp Thr Ser

245 250 255

Ala Asp Asn Glu Asn Glu Tyr Val Glu Leu Glu Ile Cys Asp Ser Leu

260 265 270

Asn Phe Ser Thr Ser His Arg Thr Val Leu Asn Asp Tyr Gly Tyr Asp

275 280 285

Gln Asn Asn Glu Gly Ile Phe Met Val Val Glu Pro Glu Asn Val Pro

290 295 300

Tyr Ser Asn Ala Ser Leu Phe Ser Tyr Phe Asp Ile Pro Ser Arg Asp

305 310 315 320

Thr Leu Thr Leu Ser Lys Tyr Ser Asp Ser Ile Ser Gln Cys Thr Thr

325 330 335

Gln Ile Leu Lys Ala Asn Ser Thr Phe Val Ala His Lys Tyr Ile Gly

340 345 350

Ile Ile Ser Ser Glu Tyr Asp Asn Lys Gln Pro Asp Asp Asn Ser Asn

355 360 365

Gly Thr Ser Asn Glu Ala Leu Lys Met Ser Asn Leu Glu Arg Ala Thr

370 375 380

Ser Ile Val Leu Asp Asn Lys Gly Asn Tyr Asp Ser Leu Ile Gln Ser

385 390 395 400

His Lys Asn Ala Trp Lys Arg Ile Tyr Lys Asp Ala Ser Ile Glu Ile

405 410 415

Pro Ser Asp Gly Leu Leu Glu Met Thr Ala Lys Ser Ser Ile Tyr His

420 425 430

Leu Leu Ala Asn Thr Arg Ser His Asn Val Ser Glu Asp Arg Gly Leu

435 440 445

Pro Ile Gly Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val

450 455 460

Phe Trp Asp Ser Asp Leu Trp Ile Leu Pro Ala Leu Leu Pro Phe Phe

465 470 475 480

Pro Asn Ala Ala Arg Gln Ile Asn Asn Tyr Arg Asn Ala Ser Leu His

485 490 495

Gln Ala Lys Leu Asn Ala Glu Lys Tyr Gly Tyr Asp Gly Ala Leu Tyr

500 505 510

Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys

515 520 525

Val Asp Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ser Ser Phe

530 535 540

Ala Ile Tyr Met Asn Gly Asp Glu Asp Asp Glu Arg Ser Glu Glu Tyr

545 550 555 560

Leu Arg Tyr Thr Thr Trp Pro Phe Val Glu Asn Ala Ala Lys Met Phe

565 570 575

Ala Gln Tyr Val Lys Trp Asn Asp Thr Met Gln Gln Tyr Thr Thr His

580 585 590

Asn Leu Thr Asp Pro Asp Glu Tyr Ala Asn Phe Val Asp Asn Ala Ala

595 600 605

Phe Thr Asn Ala Gly Ile Gln Ser Val Met Val Trp Ala His Asp Ile

610 615 620

Ala Arg His Leu Gly Ile Asp Pro Asp Pro Gln Trp Leu Glu Ile Ala

625 630 635 640

Asp Asn Ile His Ile Pro Ile Ser Glu Thr Asn Ile Thr Leu Glu Tyr

645 650 655

Ser Gly Met Asn Ser Ser Val Glu Ile Lys Gln Ala Asp Val Val Leu

660 665 670

Met Ile Tyr Pro Leu Ser Tyr Ile Thr Asp Gln Ser Ile Leu Asn Asn

675 680 685

Ala Ile Lys Asn Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly

690 695 700

Pro Ala Met Thr Tyr Pro Val Phe Val Ala Gly Ala Ala Ser Leu Leu

705 710 715 720

Asn Tyr Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Val Pro

725 730 735

Tyr Leu Arg Ser Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn

740 745 750

Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala

755 760 765

Asn Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg

770 775 780

Tyr Ser Tyr Glu Val Asp Glu Asp Thr Gly Lys Ile His Arg Leu Leu

785 790 795 800

Lys Phe Asn Pro Ile Gln Leu Pro Leu Leu Pro Gly Gly Ile Arg Ile

805 810 815

Asn Asn Phe Lys Tyr Met Gly Gln Val Leu Asp Val Leu Ile Thr Asp

820 825 830

Thr Glu Gly Ile Ile Ile His Lys Asn Gly Thr Lys Glu Ile Arg Ile

835 840 845

Lys Val Pro Asp Arg Thr Leu Ile Pro Asp Val Asp Val Lys Tyr Ser

850 855 860

Glu Glu Thr Asp Pro Ile Lys Gln Ile Leu His Gly Arg Arg Ser Val

865 870 875 880

Pro Thr Gly Lys Asn Tyr Phe Thr Ile Gln Pro Gly Asp Val Phe Lys

885 890 895

Thr Pro Leu Tyr Ile Pro Lys Lys Asn Leu Glu Gly Asn Leu Val Glu

900 905 910

Ala Lys Gln Ile Thr Asn Leu Thr Ala Gly Val Pro Gly Asp Val Ala

915 920 925

Val Ser Val Ile Asp Gly Asn Asn Phe Thr His Trp Gln Pro Ala Tyr

930 935 940

Lys Asn Leu Pro Ala Arg Leu Leu Ile Asp Met Gly Asn Asn Phe Thr

945 950 955 960

Gln Glu Ile Lys Ser Gly Lys Ile Ile Trp Gly Ser Arg Pro Ala Lys

965 970 975

Thr Phe Ser Leu Ser Ile Leu Pro Gln Thr Glu Glu Val Phe Lys Asn

980 985 990

Leu Thr His Ile Leu Ser Asn Val Asn Gln Tyr Cys Asn Lys Thr Gly

995 1000 1005

Asp Glu Cys Val Arg Asp Leu Glu Arg Thr Glu Lys Gly Phe Asp

1010 1015 1020

Ala Ala Ile Glu Asp Val Phe Asp Trp Tyr Gly Ile Asp Pro Asp

1025 1030 1035

Ser Ile Ile Ser Thr His Pro Glu Leu Lys Asp Met Lys Thr Lys

1040 1045 1050

Phe Val Lys Ile Leu Asp His Tyr Lys Val Thr Pro Ser Glu Pro

1055 1060 1065

Tyr Pro Trp Arg Val Tyr Asn Glu Ser Gln Ile Val Leu Leu Pro

1070 1075 1080

Gly Asn Glu Thr Asp Phe Asp Ile Asp Tyr Ser Lys Val Ala Glu

1085 1090 1095

Met Asn Pro Glu Asn Val Asp Ile Asp Phe Arg Gly Asn Gln Thr

1100 1105 1110

Asp Trp Arg Lys Gly Arg Phe Ile Val Leu Thr Val His Asp Thr

1115 1120 1125

Tyr Asp Asp Asp Asp Asp Glu Lys Gly Ala Thr Ile Lys Glu Phe

1130 1135 1140

Ala Leu Phe Pro

1145

<210> 2

<211> 1071

<212> PRT

<213> 近平滑假丝酵母(Candida parapsilosis)

<400> 2

Met Asp Ile Glu Asn Asn Gly Ile Tyr Met Glu Asn Ser Ser Arg Ile

1 5 10 15

Lys Gly Ile Arg Arg Leu Phe Lys Asp Asn Lys Thr Tyr Ile Ile Ala

20 25 30

Met Thr Ile Ile Ala Asn Phe Ile Leu Phe Tyr Ser Leu His Leu Leu

35 40 45

Asn Thr Arg Phe Ser Glu Ala Phe Pro Phe Val Leu Pro Arg Ala Glu

50 55 60

Lys Tyr Thr Leu Glu Gln Thr Ser Asp Ser Asn Leu Gly Asp Phe Val

65 70 75 80

Asn Ser Phe Glu Asn Lys Glu Ile Phe Ser Gln Val Gln Phe Ser Glu

85 90 95

His Ala Phe Tyr Asp Arg Lys Met Asn Val Val Gly Thr Ile Glu Tyr

100 105 110

Ser Lys Tyr Asn Gln Phe Gln Lys Gln Pro Tyr Val Ala Asn Gly Tyr

115 120 125

Ile Gly Ser Arg Ile Pro Asn Leu Gly His Gly Phe Thr Tyr Asp Gln

130 135 140

Leu Thr Glu Ser Pro Asp Ser Lys Lys Asp Asp Leu Tyr Asn Gly Trp

145 150 155 160

Pro Leu Phe Asp Lys Arg Phe Ser Gly Ala Phe Ile Ala Gly Phe Tyr

165 170 175

Asp Leu Gln Glu Asn Thr Thr Gly Asn Asn Phe Pro Glu Leu Leu Lys

180 185 190

Asn Gly Tyr Glu Ser Val Ile Ser Ala Ile Pro Gln Trp Thr Thr Leu

195 200 205

Lys Leu Ala Ile Gln His Gly Asp Lys Arg Tyr Thr Leu Asp Pro Ala

210 215 220

Ser Pro Asp Gly Val Gly Gln Ile Thr Asn Tyr Val Gln Asn Met Ser

225 230 235 240

Leu Ser Thr Gly Ile Val Thr Thr Gln Phe Thr Trp Leu Glu Ser Leu

245 250 255

Asp Val Arg Tyr Thr Val Leu Ala His Arg Ser Glu Ile Asn Leu Gly

260 265 270

Leu Val Lys Val Asp Ile Lys Asn Lys Gly Asn Asp Thr Val Glu Ile

275 280 285

Asp Val Ile Asp Glu Leu Asn Phe Asp Thr Ala Gln Arg Cys Ser Leu

290 295 300

Asn Leu Ile Gly His Asp Asn Ser Gly Ile Tyr Val Ser Phe Ser Pro

305 310 315 320

Asp Asn Leu Asp Tyr Ile Ser Gly Ala Ile Tyr Ser Thr Leu His Leu

325 330 335

Asp Ser Val Lys Arg His Ser Lys Asn Lys Lys Thr Asn Gln Thr Ser

340 345 350

Ser Ile Arg Leu His Ala Gln Asp Ser Thr Gln Phe Gly Lys Thr Val

355 360 365

Gly Ile Val Ser Thr Asp Leu Asp Pro Ser Lys Phe Lys Asn Ser Arg

370 375 380

Asp Thr Leu Glu Phe Ala Lys Lys Val Ser Gln Lys Phe Glu Tyr Leu

385 390 395 400

Asp Leu Val Ile Lys Ser His Val Arg Ala Trp Lys Glu Leu Met Gly

405 410 415

Lys Gln Ser Thr Ile Thr Phe Pro Asn Asp Pro Leu Leu Asp Leu Gly

420 425 430

Ser Lys Ala Ser Val Tyr His Leu Leu Ala Asn Thr Arg Pro Asp Ala

435 440 445

Gln Gly Leu Thr Gly Ala Leu Gly Val Ala Gly Leu Ser Ser Asp Ser

450 455 460

Tyr Gly Gly Met Val Phe Trp Asp Thr Asp Phe Trp Met Phe Arg Ala

465 470 475 480

Ile Leu Ala Leu Asp Pro Thr His Ala Lys Ser Ile Val Asn Tyr Arg

485 490 495

Met His Thr His Gln Gln Ala Leu Lys Asn Thr Pro Gln Gly Tyr Glu

500 505 510

Gly Ala Ala Tyr Pro Trp Thr Ser Gly Arg Phe Gly Asn Cys Thr Ala

515 520 525

Thr Gly Pro Cys Leu Asn Tyr Glu Tyr His Ile Asn Ser Ala Ile Ala

530 535 540

Met Ala Ala Trp Glu Leu Tyr Ile Ser Gly Ala Val Asp Asp Gln Tyr

545 550 555 560

Leu Glu Ser Thr Val Tyr Pro Leu Ile Ser Asp Ala Ala Lys Phe Tyr

565 570 575

Ala Asp Tyr Val Thr Ser Tyr Asn Glu Thr Ile Asp Lys Phe Ile Thr

580 585 590

Ser Asn Leu Thr Asp Pro Asp Glu Tyr Ala Asn His Val Asp Asn Gly

595 600 605

Ala Tyr Thr Asn Ala Gly Ile Ala Leu Leu Met Asn Trp Ile Asn Asp

610 615 620

Ile Gly Asp Ile Leu Gly Ile Lys Met Pro Glu Ile Tyr Thr Asp Ile

625 630 635 640

Ser Gln Lys Met Tyr Leu Pro Thr Ala Asp Asn Ser Gln Asn Ile Thr

645 650 655

Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Gly Ile Lys Gln Ala Asp

660 665 670

Val Val Leu Met Thr Tyr Pro Leu Gln Asn Ser Leu Val Asp Asp Glu

675 680 685

Gln Ala Tyr Ile Asn Met Glu Phe Tyr Ser Gly Lys Gln Val Ser Tyr

690 695 700

Gly Pro Ala Met Thr Phe Ser Ile Phe Ser Ile Val Ala Ala Asn Leu

705 710 715 720

Ala Thr Ser Gly Cys Ala Ser Gln Ser Tyr Leu His Lys Ala Met Gln

725 730 735

Pro Tyr Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Asn Asn Asp

740 745 750

Asn Phe Lys Thr Asn Gly Gly Thr His Pro Ala Tyr Pro Phe Leu Thr

755 760 765

Ala His Gly Gly Phe Ile Gln Ala Val Val Asn Gly Leu Thr Gly Leu

770 775 780

Arg His Ser Tyr Thr Ile Asp Asn Gly Lys Ile Arg Arg Ser Leu Phe

785 790 795 800

Leu Asp Pro Ile Ala Leu Pro Cys Leu Gly Asp Gly Val Gln Tyr Ser

805 810 815

Gly Ile His Tyr Asp Asn His Thr Ile Ser Met Asn Ile Thr Gly Ser

820 825 830

Thr Phe Val Ile Arg Asn Glu Gly Lys Thr Asn Lys Arg Ala Asn Asp

835 840 845

Tyr Ile Ser Ile Thr Ile Ala Asp Arg Asn Pro Gly Arg Gly Thr Tyr

850 855 860

Lys Leu Lys Asp Asn Glu Glu Lys Ser Phe Lys Leu Tyr Gln Pro Ser

865 870 875 880

Lys Ile Phe Glu Asp Ser Val Ser Glu Cys Gly Leu Ala Thr Phe Tyr

885 890 895

Asn Ile Thr Glu Ser Ala Pro Gly Asp Ser Ser Phe Ser Ile Asn Asp

900 905 910

Gly Asp Asn Thr Thr Arg Trp Gln Val Lys Tyr Asn Asp Thr Thr Gly

915 920 925

Lys Val Leu Ile Asp Leu His Ser Ile Lys Asn Ile Thr Gly Val Val

930 935 940

Phe Asn Trp Ala Asp Lys Pro Pro Lys Lys Val Lys Leu Ser Lys Tyr

945 950 955 960

Met Gly Thr Lys Phe Lys Asp Val Thr Asp Phe Leu Ala Lys Val Asp

965 970 975

Phe Gly Asn Tyr Val Tyr Lys Asn Tyr Arg Phe Ala Asn Pro Gly Glu

980 985 990

Lys Leu Leu Asn Gln Ser Glu Val Phe Glu Asn Val Tyr Ser Asp Asp

995 1000 1005

Val Thr Ile Asn Ala Pro Phe Ser Ser Lys Glu Leu Glu Glu Val

1010 1015 1020

Ile Val Pro Val Arg His Asn Thr Thr Ser Trp Asn Ile Asn Leu

1025 1030 1035

Gln Gly Arg Phe Leu Leu Leu Glu Val Asp Gly Ile His Asn Thr

1040 1045 1050

Ile Pro Ile Glu Asp Asp Tyr Gly Gly Ala Lys Leu Ala Glu Val

1055 1060 1065

Asn Phe Phe

1070

<210> 3

<211> 1212

<212> PRT

<213> 光滑假丝酵母(Candida glabrata)

<400> 3

Met Gly Phe Lys Asp Lys Ile Leu Phe Trp Lys Asp Glu Val Gln Tyr

1 5 10 15

Arg Thr Leu Ala Val Ala Asp Gln Val Ala Asn Arg Phe Phe His Ser

20 25 30

Phe Glu Asn Val Tyr Lys Gly Asp Glu Ser Val Glu Asp Ala Asp Ser

35 40 45

Arg Pro Val Gly Leu Thr Asn Glu Thr Leu Ser His Ser Ser Asp Phe

50 55 60

Phe Val Leu Pro Glu Glu Arg Ile Ser Thr Arg Val Lys Ile Arg Arg

65 70 75 80

Gln Asn Ile Leu Asn Thr Thr Leu Ile Leu Gly Met Leu Ile Ala Leu

85 90 95

Val Ile Trp Thr Ala Ile Leu Ser Thr Asn Ser Tyr Phe Ser Ser Ser

100 105 110

Leu Ala Ser Ala Ser Pro Leu Phe Asn Lys Glu Gly Arg Val Val Arg

115 120 125

Pro Met Arg Glu Ser Asn Leu Gly Leu His Ala Asp Pro Gln Thr Arg

130 135 140

Lys Ser Ser Lys Thr Leu Tyr Asp Leu Leu Ser Asp Phe Asp Asn Ala

145 150 155 160

Phe Tyr Asp Asp Glu Asn Met Ile Leu Gly Ser Leu Ala Phe Gly Glu

165 170 175

Asn Thr Tyr Ser Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser

180 185 190

Arg Ile Pro Asn Ile Gly Phe Gly Tyr Ala Leu Asp Thr Leu Asn Leu

195 200 205

Tyr Ala Asp Ala Pro Gly Ala Leu Asn Asn Gly Trp Pro Leu Arg Asn

210 215 220

Arg Arg Phe Ala Gly Ser Phe Val Ser Asp Phe Tyr Ser Leu Gln Ala

225 230 235 240

Lys Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp Glu Lys Gly Tyr Thr

245 250 255

Thr Val Ile Ser Ser Ile Pro Glu Trp Thr Asp Leu Gln Phe Thr Val

260 265 270

Asp Leu Asn Gly Thr Asn Trp Phe Asn Pro Gln Ser Val Leu Ile Asp

275 280 285

Asp Val Ile Asn Tyr Asn Gln Asn Leu Ser Met Lys Asp Gly Ile Val

290 295 300

Ser Thr Asn Met Asp Trp Leu Asn Gly Met Ile Asn Ile Lys Ser Glu

305 310 315 320

Val Trp Ala His Arg Lys Ile His Ser Leu Gly Ile Thr Arg Leu Glu

325 330 335

Ile Ser Leu Asn Leu Asp Ala Leu Pro Asp Glu Phe Thr Glu Leu Pro

340 345 350

Val Thr Ile Tyr Asp Ile Ile Asp Phe Asn Thr Ser His Arg Thr Thr

355 360 365

Leu Tyr Glu Lys Gly Gln Asp Glu Asp Asn Lys Ala Ile Tyr Met Ile

370 375 380

Val Asn Pro Glu Asn Val Pro Tyr Ser Asn Ala Val Val Tyr Ser Thr

385 390 395 400

Cys Thr Ile Lys Gly Thr Glu Asn Asn Phe Ser Pro Tyr Asn Phe Thr

405 410 415

Ser Asp Asp Arg Ile Ala Arg Asn Tyr Met Thr Asn Leu Thr Glu Glu

420 425 430

Asn Pro Lys Val Val Ile Tyr Lys Tyr Thr Ser Val Val Ser Ser Glu

435 440 445

Tyr Asn Asn Asp Glu Pro Asn Pro Asn Val Asn Leu Lys Phe Ala Ser

450 455 460

Asn Ile Ala Asn Thr Ala Lys Gly Asn Tyr Lys Ser Leu Leu Ser Asn

465 470 475 480

His Lys Arg Ala Trp Tyr Asp Leu Tyr Asn Asp Ala Phe Ile Glu Ile

485 490 495

Pro Ser Asp Ser Leu Leu Glu Met Thr Ala Arg Ser Ser Leu Phe His

500 505 510

Leu Leu Ala Asn Thr Arg Gln Tyr Asn Val Ser Thr Thr Arg Gly Leu

515 520 525

Pro Val Gly Val Gly Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val

530 535 540

Phe Trp Asp Ala Asp Val Trp Met Ala Pro Ala Leu Leu Pro Phe Phe

545 550 555 560

Pro Asn Ile Ala Met Asn Met Asn Asn Tyr Arg Asn Ala Thr His Gln

565 570 575

Gln Ala Ile Glu Asn Ala Lys Gln Tyr Asn Tyr Pro Gly Ala Val Tyr

580 585 590

Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys

595 600 605

Ile Asp Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ala Ser Phe

610 615 620

Ser Ile Tyr Met Asn Gly Ala Glu Gly Ala Asp Glu Asp Tyr Leu Arg

625 630 635 640

Phe Thr Thr Trp Pro Met Val Lys Asp Ala Ala Val Phe Phe Lys Ala

645 650 655

Tyr Val Lys Tyr Asn Glu Thr Leu Gly Glu Tyr Glu Thr Tyr Asn Leu

660 665 670

Thr Asp Pro Asp Glu Phe Ala Asn His Val Asn Asn Gly Ala Phe Thr

675 680 685

Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp Ala Thr Asp Ile Gly Thr

690 695 700

His Leu Gly Glu Glu Val Asp Pro Lys Trp Met Glu Ile Ala Asp Asn

705 710 715 720

Ile His Ile Pro Arg Ser Asp Ser Asn Ile Thr Leu Glu Tyr Ser Gly

725 730 735

Met Asn Ser Ser Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val

740 745 750

Tyr Pro Leu Gly Tyr Ile Asn Asp Glu Ser Ile Leu Asn Asn Ala Ile

755 760 765

Lys Asp Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala

770 775 780

Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Ser Leu Leu Asn His

785 790 795 800

Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Leu Pro Tyr Leu

805 810 815

Arg Ser Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu

820 825 830

Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly

835 840 845

Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser

850 855 860

Tyr Glu Val Thr Pro Arg Thr Lys Lys Ile Ser Arg Leu Leu Lys Phe

865 870 875 880

Asp Pro Val Lys Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn

885 890 895

Phe Lys Tyr Met Gly Gln Val Leu Asp Ile Ile Ile Asp Asp Asn Asn

900 905 910

Gly Thr Ile Ala His Lys Gly Gly Asp Lys Pro Ile Arg Ile Lys Val

915 920 925

Pro Asn Arg Asp Ile Leu His Asp Arg Asn Ile Thr Ser Ala Leu Tyr

930 935 940

Ser Lys Arg Asp Asp Asp Leu Ser Ala Thr Asp Asp Tyr Tyr Gly Thr

945 950 955 960

Tyr Phe Thr Leu Tyr Pro Asn Glu Glu Leu Val Ile Pro Leu Tyr Asp

965 970 975

Thr Lys Leu Asn Ile Asp Gly Asn Ile Ala Glu Ser Lys Gln Ile Thr

980 985 990

Asn Leu Thr Ala Gly Val Pro Gly Asp Val Gly Phe Ser Ala Leu Asp

995 1000 1005

Gly Asn Asn Tyr Thr His Trp Gln Pro Phe Asp Lys Ser Asp Asn

1010 1015 1020

Ala Lys Leu Leu Ile Asp Leu Gly Phe Asn Ser Thr His Val Ile

1025 1030 1035

Lys Lys Gly Ile Ile Leu Trp Gly Gln Arg Pro Ala Lys Asn Ile

1040 1045 1050

Ser Leu Ser Val Leu Pro His Ser Glu Arg Ile Glu Gln Leu Phe

1055 1060 1065

Ala Asn Ile Thr Asp Leu Leu Glu Thr Ser Ser Ile Thr Lys Gly

1070 1075 1080

Gly Ser Leu Leu Asn Gln Met Leu Gly Gln Thr Gln Ser Asn Val

1085 1090 1095

Thr Ala Glu Ile Asp Asp Asp Ile Leu Ala Leu Leu Asn Trp Lys

1100 1105 1110

Gly Asp Asp Leu Asp Gln Leu Ile Pro Tyr Leu Pro Asp Met His

1115 1120 1125

Leu Leu Gln Glu Lys Phe Ile Pro Ile Leu Lys Asp Tyr Pro Ile

1130 1135 1140

Lys Pro Asn Gln Arg Tyr Tyr Lys Glu Ile Ile Asp Asp Asp Ile

1145 1150 1155

Ile Lys Leu Leu Pro Ser Asn Thr Thr Glu Phe Thr Ile Asp Tyr

1160 1165 1170

Asn Ser Ile Pro Gly Gly Glu Lys Arg Ala Arg Tyr Val Val Leu

1175 1180 1185

Thr Val His Gly Thr Tyr Asp Asp Asp Asp Asp Leu Lys Gly Ala

1190 1195 1200

Thr Ile Lys Glu Ile Val Leu Gln Glu

1205 1210

<210> 4

<211> 19

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 4

Met Lys Phe Ile Ser Thr Phe Leu Thr Phe Ile Leu Ala Ala Val Ser

1 5 10 15

Val Thr Ala

<210> 5

<211> 683

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 5

atctcccgag tttatcatta tcaatactgc catttcaaag aatacgtaaa taattaatag 60

tagtgatttt cctaacttta tttagtcaaa aaattggcct tttaattctg ctgtaacccg 120

tacatgccca aaataggggg cgggttacac agaatatata acatcatagg tgtctgggtg 180

aacagtttat tcctggcatc cactaaatat aatggagccc gcttttttta agctggcatc 240

cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca tcagttcata 300

ggtccattct cttagcgcaa ctacacagaa caggggcaca aacaggcaaa aaacgggcac 360

aacctcaatg gagtgatgca acctgcttgg agtaaatgat gacacaaggc aattgaccta 420

cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct ctgatttgga 480

aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctat ttgactaata 540

agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt aaacttctta 600

aattctactt ttatagttag tctttttttt agttttaaaa cactaagaac ttagtttcga 660

ataaacacac ataaacaaac aaa 683

<210> 6

<211> 5745

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多个单独的酿酒酵母序列共同组成的序列

<400> 6

tcgagatctc ccgagtttat cattatcaat actgccattt caaagaatac gtaaataatt 60

aatagtagtg attttcctaa ctttatttag tcaaaaaatt ggccttttaa ttctgctgta 120

acccgtacat gcccaaaata gggggcgggt tacacagaat atataacatc ataggtgtct 180

gggtgaacag tttattcctg gcatccacta aatataatgg agcccgcttt ttttaagctg 240

gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc aaccatcagt 300

tcataggtcc attctcttag cgcaactaca cagaacaggg gcacaaacag gcaaaaaacg 360

ggcacaacct caatggagtg atgcaacctg cttggagtaa atgatgacac aaggcaattg 420

acctacgcat gtatctatct cattttctta caccttctat taccttctgc tctctctgat 480

ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg aaattattcc cctatttgac 540

taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat ttcttaaact 600

tcttaaattc tacttttata gttagtcttt tttttagttt taaaacacta agaacttagt 660

ttcgaataaa cacacataaa caaacaaatc tagattaatt aaacaggccc cttttccttt 720

gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc cacatccgct 780

ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 840

gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 900

cgcgtgtacg catgtaacgg gcagacgcgg ccgccaccgc ggtggagctc caattcgccc 960

tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 1020

cctggcgtta cccaacttaa tcgccttgca gcacatcccc ccttcgccag ctggcgtaat 1080

agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 1140

cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 1200

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 1260

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 1320

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 1380

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 1440

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 1500

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 1560

tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg tattttctcc 1620

ttacgcatct gtgcggtatt tcacaccgca gggtaataac tgatataatt aaattgaagc 1680

tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg 1740

gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt 1800

agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga 1860

gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc 1920

cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg 1980

agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt 2040

atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct 2100

aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac 2160

cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta 2220

ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt 2280

gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa 2340

gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag 2400

taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat 2460

gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt 2520

agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa 2580

gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag 2640

tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca 2700

aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagcgtg 2760

gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 2820

aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 2880

tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 2940

gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 3000

ttcttaggac ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa 3060

taatttggga atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa 3120

gtaaataaag aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa 3180

aaatttcaac aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa 3240

tagatataca ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt 3300

cttctacaca gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga 3360

taaaaggtag tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa 3420

ctattttttc tttaatttct ttttttactt tctattttta atttatatat ttatattaaa 3480

aaatttaaat tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc 3540

ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 3600

cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 3660

gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 3720

ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 3780

tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 3840

aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 3900

ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 3960

agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 4020

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 4080

gaccgaagga gctaaccgct ttttttcaca acatggggga tcatgtaact cgccttgatc 4140

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 4200

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 4260

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 4320

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 4380

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 4440

cgggcagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 4500

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 4560

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4620

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4680

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4740

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 4800

ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 4860

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 4920

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 4980

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 5040

gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc 5100

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 5160

cgagggagct tccagggggg aacgcctggt atctttatag tcctgtcggg tttcgccacc 5220

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gccgagccta tggaaaaacg 5280

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 5340

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 5400

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 5460

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 5520

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttacctca 5580

ctcattaggc accccaggct ttacacttta tgcttccggc tcctatgttg tgtggaattg 5640

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcggaa 5700

ttaaccctca ctaaagggaa caaaagctgg gtaccgggcc ccccc 5745

<210> 7

<211> 9181

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体乳酸克鲁维酵母和酿酒酵母共同组成的序列

<400> 7

gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 60

tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 120

ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 180

taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 240

gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 300

aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 360

gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 420

ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 480

ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gctttttttc 540

acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 600

taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 660

tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 720

cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 780

ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 840

gtaagccctc ccgtatcgta gttatctaca cgacgggcag tcaggcaact atggatgaac 900

gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 960

aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 1020

aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 1080

actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 1140

gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 1200

atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 1260

atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 1320

ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 1380

gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 1440

cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 1500

tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 1560

cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg gggaacgcct 1620

ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 1680

gctcgtcagg ggggccgagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 1740

tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 1800

ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 1860

gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 1920

cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 1980

gtgagcgcaa cgcaattaat gtgagttacc tcactcatta ggcaccccag gctttacact 2040

ttatgcttcc ggctcctatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 2100

acagctatga ccatgattac gccaagctcg gaattaaccc tcactaaagg gaacaaaagc 2160

tgggtaccgg gccccccctc gagatctccc gagtttatca ttatcaatac tgccatttca 2220

aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattgg 2280

ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat 2340

ataacatcat aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag 2400

cccgcttttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt 2460

tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaca gaacaggggc 2520

acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgct tggagtaaat 2580

gatgacacaa ggcaattgac ctacgcatgt atctatctca ttttcttaca ccttctatta 2640

ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa 2700

attattcccc tatttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt 2760

aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta 2820

aaacactaag aacttagttt cgaataaaca cacataaaaa caaatctaga atgaagttca 2880

tttcaacttt cttgactttt atcttggctg ccgttagtgt tactgctacc aaacctttac 2940

cattacaaca tactcatttt ggctcttttg tctgcgctga ggatacctta ttctgccctg 3000

aatctcagag aaaagcctct gaaaagatgt atagtttatt gaaggatcac gaaaacacat 3060

tctacgatga ggaacagcaa atcctgggaa acttattgtt atcagaaaat acattctcaa 3120

gacaaccata tgttgcaaac ggttacattg gatccagaat ccctaacctt gggttcggat 3180

acgcacttga taccattaac gtttgggtaa atgactcctc tataccaggc gcccttgata 3240

atggttggcc attgagaaac cagagatttg ctggggcatt catttcagac ttctactgtc 3300

tacaagagaa gctcaactca actaacttcc ctgaattgga cgatgatggt tactcaactg 3360

ttatatcaac aataccacaa tggacagatc tctcaatttt aaagcacact actacaggtc 3420

aaatcgagta cattaatcct acagatgtta agttagataa gattacaaac tatatgcaaa 3480

acttatcact tcaagatgga attgtaacaa ctacatttgt ttacgacaaa caactattgg 3540

ttactacaaa ggtagttgca catagaaaga tataccctct cggagtcgtt accttagaac 3600

tatctttgtt cgacaacaat tctgatacta gtgctgacaa cgaaaacgaa tacgtcgaat 3660

tagagatctg tgattcacta aacttctcta catcccacag aaccgtactt aatgattacg 3720

gttacgatca aaacaacgag ggtattttca tggtggtgga gccagaaaac gtcccatatt 3780

ctaatgcatc ccttttttca tattttgata tcccaagtag agatacatta acactttcta 3840

agtactccga ttcaatcagt caatgtacaa cacaaatctt aaaggcaaat tcaacttttg 3900

tagctcacaa gtacatcggt ataattagct ctgagtacga taacaaacaa cctgatgata 3960

actctaatgg aacttctaat gaagcgctta aaatgtctaa cctggaacgt gcaaccagta 4020

ttgtgcttga taacaaaggt aattacgatt ctctgattca atcacataaa aatgcttgga 4080

agagaattta caaagatgct agcattgaaa taccatccga tggtttgctg gagatgacag 4140

caaaaagttc tatttaccat ttactagcaa atacaagatc tcataacgtg tctgaggaca 4200

gaggtttgcc tataggcgtg tcaggattat cttctgattc ttacgggggt atggttttct 4260

gggattctga cttgtggatt ctaccagcac tcctgccatt cttcccaaac gctgcaagac 4320

agatcaataa ctacagaaat gcaagccttc accaggccaa gctaaacgca gaaaagtacg 4380

gctacgatgg cgcgctatac ccatggacat ctggcagata cgcgaactgt acttcaactg 4440

gtccatgtgt cgactacgaa tatcatatca acgtggacat cgctttgtca tctttcgcca 4500

tctacatgaa tggtgatgaa gatgacgaac gttccgaaga gtacttgcgt tacacaacat 4560

ggccattcgt agaaaacgca gcaaagatgt ttgctcaata cgttaagtgg aatgacacaa 4620

tgcaacaata caccactcat aacttgactg atccagatga atatgcaaac ttcgttgaca 4680

acgctgcatt tactaatgct ggtatacaat ctgtgatggt ctgggctcat gatatcgcca 4740

gacacttggg catcgatcca gatccacagt ggttggagat agccgacaac atccatattc 4800

caatttccga gacaaacatc acattggaat actcaggtat gaactcctct gtcgaaatca 4860

agcaagctga tgtggtattg atgatttacc cattatctta cataacagat caaagcatct 4920

tgaacaacgc aattaaaaac ttgtactact attctgaacg tcaaagtgca tcagggcctg 4980

ctatgacata cccagtattt gtcgccggag cggcatctct tcttaactac ggttcatctt 5040

cacaatccta tctatacaag tcagttgtcc catacttacg tagtcctttt gctcaattct 5100

cagaacaaag tgacgacaac ttcttgacca atggtctaac acagccagcg ttcccatttc 5160

taacagctaa cggtggtttc ttgcagtcta ttctattcgg tttgactggg ctcagataca 5220

gttacgaagt tgatgaagat acagggaaaa tccatagatt actgaagttc aatccaattc 5280

agttgcctct cctacctgga ggtattagaa ttaacaactt taagtacatg ggtcaggtat 5340

tagatgtttt gatcacagac accgagggta tcatcatcca caaaaacggt actaaggaaa 5400

tcagaatcaa agttccagat agaactctaa ttcctgatgt agatgtcaag tactcagaag 5460

agactgatcc tattaagcaa attttgcatg gtagaagatc tgttccaacc ggtaaaaact 5520

acttcactat acaacctggt gatgtgttca agacaccact ttacatccct aaaaaaaact 5580

tggaaggcaa tttggtcgag gctaagcaaa ttaccaattt gactgccggc gtcccaggtg 5640

acgttgctgt ctctgtaata gatggaaaca actttacaca ctggcaacca gcatacaaaa 5700

accttccagc cagattgtta atcgatatgg gcaataattt tacccaagag attaagtccg 5760

gcaagatcat atggggctca agaccagcaa aaacattctc cctgtcaatt ttgccacaga 5820

cagaagaggt tttcaaaaat cttacacata ttttatctaa cgttaatcaa tattgtaaca 5880

agacaggtga tgagtgcgtc agagatttag agagaacaga gaaagggttc gatgccgcaa 5940

tagaagatgt tttcgactgg tacggtattg acccagacag cattatctca acacatcctg 6000

aattaaaaga tatgaaaaca aagttcgtca agatcctaga tcactacaaa gtcactccat 6060

ccgaacctta tccatggaga gtttacaacg agtctcaaat cgtgctgctt ccaggcaatg 6120

aaacagattt cgatattgat tactctaaag ttgctgaaat gaaccctgaa aacgttgata 6180

ttgatttcag agggaaccag acagattgga gaaagggaag atttatcgtt ctcacagtgc 6240

atgatactta cgacgacgat gatgacgaaa agggtgccac tatcaaggaa tttgcattat 6300

tcccttgatt aattaaacag gccccttttc ctttgtcgat atcatgtaat tagttatgtc 6360

acgcttacat tcacgccctc ctcccacatc cgctctaacc gaaaaggaag gagttagaca 6420

acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt 6480

tatatttcaa atttttcttt tttttctgta caaacgcgtg tacgcatgta acgggcagac 6540

gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt acaattcact 6600

ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 6660

tgcagcacat ccccccttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 6720

ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt 6780

aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 6840

gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 6900

agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 6960

caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 7020

tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 7080

aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 7140

ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 7200

aacgtttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 7260

cgcagggtaa taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg 7320

catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc 7380

agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc 7440

ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg gttctatact 7500

gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat 7560

cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt 7620

tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct 7680

tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg 7740

cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg 7800

cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt 7860

cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg 7920

gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac 7980

aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca 8040

atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag 8100

gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt 8160

tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt 8220

caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct 8280

tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa ccgaaatcaa aaaaaagaat 8340

aaaaaaaaaa tgatgaattg aattgaaaag cgtggtgcac tctcagtaca atctgctctg 8400

atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 8460

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 8520

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 8580

tatttttata ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta 8640

acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta 8700

tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac 8760

ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac 8820

atatatattt attagacaag aaaagcagat taaatagata tacattcgat taacgataag 8880

taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat 8940

tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc 9000

ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt 9060

actttctatt tttaatttat atatttatat taaaaaattt aaattataat tatttttata 9120

gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 9180

t 9181

<210> 8

<211> 1145

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<400> 8

Met Lys Phe Ile Ser Thr Phe Leu Thr Phe Ile Leu Ala Ala Val Ser

1 5 10 15

Val Thr Ala Thr Lys Pro Leu Pro Leu Gln His Thr His Phe Gly Ser

20 25 30

Phe Val Cys Ala Glu Asp Thr Leu Phe Cys Pro Glu Ser Gln Arg Lys

35 40 45

Ala Ser Glu Lys Met Tyr Ser Leu Leu Lys Asp His Glu Asn Thr Phe

50 55 60

Tyr Asp Glu Glu Gln Gln Ile Leu Gly Asn Leu Leu Leu Ser Glu Asn

65 70 75 80

Thr Phe Ser Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg

85 90 95

Ile Pro Asn Leu Gly Phe Gly Tyr Ala Leu Asp Thr Ile Asn Val Trp

100 105 110

Val Asn Asp Ser Ser Ile Pro Gly Ala Leu Asp Asn Gly Trp Pro Leu

115 120 125

Arg Asn Gln Arg Phe Ala Gly Ala Phe Ile Ser Asp Phe Tyr Cys Leu

130 135 140

Gln Glu Lys Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp Asp Asp Gly

145 150 155 160

Tyr Ser Thr Val Ile Ser Thr Ile Pro Gln Trp Thr Asp Leu Ser Ile

165 170 175

Leu Lys His Thr Thr Thr Gly Gln Ile Glu Tyr Ile Asn Pro Thr Asp

180 185 190

Val Lys Leu Asp Lys Ile Thr Asn Tyr Met Gln Asn Leu Ser Leu Gln

195 200 205

Asp Gly Ile Val Thr Thr Thr Phe Val Tyr Asp Lys Gln Leu Leu Val

210 215 220

Thr Thr Lys Val Val Ala His Arg Lys Ile Tyr Pro Leu Gly Val Val

225 230 235 240

Thr Leu Glu Leu Ser Leu Phe Asp Asn Asn Ser Asp Thr Ser Ala Asp

245 250 255

Asn Glu Asn Glu Tyr Val Glu Leu Glu Ile Cys Asp Ser Leu Asn Phe

260 265 270

Ser Thr Ser His Arg Thr Val Leu Asn Asp Tyr Gly Tyr Asp Gln Asn

275 280 285

Asn Glu Gly Ile Phe Met Val Val Glu Pro Glu Asn Val Pro Tyr Ser

290 295 300

Asn Ala Ser Leu Phe Ser Tyr Phe Asp Ile Pro Ser Arg Asp Thr Leu

305 310 315 320

Thr Leu Ser Lys Tyr Ser Asp Ser Ile Ser Gln Cys Thr Thr Gln Ile

325 330 335

Leu Lys Ala Asn Ser Thr Phe Val Ala His Lys Tyr Ile Gly Ile Ile

340 345 350

Ser Ser Glu Tyr Asp Asn Lys Gln Pro Asp Asp Asn Ser Asn Gly Thr

355 360 365

Ser Asn Glu Ala Leu Lys Met Ser Asn Leu Glu Arg Ala Thr Ser Ile

370 375 380

Val Leu Asp Asn Lys Gly Asn Tyr Asp Ser Leu Ile Gln Ser His Lys

385 390 395 400

Asn Ala Trp Lys Arg Ile Tyr Lys Asp Ala Ser Ile Glu Ile Pro Ser

405 410 415

Asp Gly Leu Leu Glu Met Thr Ala Lys Ser Ser Ile Tyr His Leu Leu

420 425 430

Ala Asn Thr Arg Ser His Asn Val Ser Glu Asp Arg Gly Leu Pro Ile

435 440 445

Gly Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp

450 455 460

Asp Ser Asp Leu Trp Ile Leu Pro Ala Leu Leu Pro Phe Phe Pro Asn

465 470 475 480

Ala Ala Arg Gln Ile Asn Asn Tyr Arg Asn Ala Ser Leu His Gln Ala

485 490 495

Lys Leu Asn Ala Glu Lys Tyr Gly Tyr Asp Gly Ala Leu Tyr Pro Trp

500 505 510

Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Val Asp

515 520 525

Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ser Ser Phe Ala Ile

530 535 540

Tyr Met Asn Gly Asp Glu Asp Asp Glu Arg Ser Glu Glu Tyr Leu Arg

545 550 555 560

Tyr Thr Thr Trp Pro Phe Val Glu Asn Ala Ala Lys Met Phe Ala Gln

565 570 575

Tyr Val Lys Trp Asn Asp Thr Met Gln Gln Tyr Thr Thr His Asn Leu

580 585 590

Thr Asp Pro Asp Glu Tyr Ala Asn Phe Val Asp Asn Ala Ala Phe Thr

595 600 605

Asn Ala Gly Ile Gln Ser Val Met Val Trp Ala His Asp Ile Ala Arg

610 615 620

His Leu Gly Ile Asp Pro Asp Pro Gln Trp Leu Glu Ile Ala Asp Asn

625 630 635 640

Ile His Ile Pro Ile Ser Glu Thr Asn Ile Thr Leu Glu Tyr Ser Gly

645 650 655

Met Asn Ser Ser Val Glu Ile Lys Gln Ala Asp Val Val Leu Met Ile

660 665 670

Tyr Pro Leu Ser Tyr Ile Thr Asp Gln Ser Ile Leu Asn Asn Ala Ile

675 680 685

Lys Asn Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala

690 695 700

Met Thr Tyr Pro Val Phe Val Ala Gly Ala Ala Ser Leu Leu Asn Tyr

705 710 715 720

Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Val Pro Tyr Leu

725 730 735

Arg Ser Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu

740 745 750

Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly

755 760 765

Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser

770 775 780

Tyr Glu Val Asp Glu Asp Thr Gly Lys Ile His Arg Leu Leu Lys Phe

785 790 795 800

Asn Pro Ile Gln Leu Pro Leu Leu Pro Gly Gly Ile Arg Ile Asn Asn

805 810 815

Phe Lys Tyr Met Gly Gln Val Leu Asp Val Leu Ile Thr Asp Thr Glu

820 825 830

Gly Ile Ile Ile His Lys Asn Gly Thr Lys Glu Ile Arg Ile Lys Val

835 840 845

Pro Asp Arg Thr Leu Ile Pro Asp Val Asp Val Lys Tyr Ser Glu Glu

850 855 860

Thr Asp Pro Ile Lys Gln Ile Leu His Gly Arg Arg Ser Val Pro Thr

865 870 875 880

Gly Lys Asn Tyr Phe Thr Ile Gln Pro Gly Asp Val Phe Lys Thr Pro

885 890 895

Leu Tyr Ile Pro Lys Lys Asn Leu Glu Gly Asn Leu Val Glu Ala Lys

900 905 910

Gln Ile Thr Asn Leu Thr Ala Gly Val Pro Gly Asp Val Ala Val Ser

915 920 925

Val Ile Asp Gly Asn Asn Phe Thr His Trp Gln Pro Ala Tyr Lys Asn

930 935 940

Leu Pro Ala Arg Leu Leu Ile Asp Met Gly Asn Asn Phe Thr Gln Glu

945 950 955 960

Ile Lys Ser Gly Lys Ile Ile Trp Gly Ser Arg Pro Ala Lys Thr Phe

965 970 975

Ser Leu Ser Ile Leu Pro Gln Thr Glu Glu Val Phe Lys Asn Leu Thr

980 985 990

His Ile Leu Ser Asn Val Asn Gln Tyr Cys Asn Lys Thr Gly Asp Glu

995 1000 1005

Cys Val Arg Asp Leu Glu Arg Thr Glu Lys Gly Phe Asp Ala Ala

1010 1015 1020

Ile Glu Asp Val Phe Asp Trp Tyr Gly Ile Asp Pro Asp Ser Ile

1025 1030 1035

Ile Ser Thr His Pro Glu Leu Lys Asp Met Lys Thr Lys Phe Val

1040 1045 1050

Lys Ile Leu Asp His Tyr Lys Val Thr Pro Ser Glu Pro Tyr Pro

1055 1060 1065

Trp Arg Val Tyr Asn Glu Ser Gln Ile Val Leu Leu Pro Gly Asn

1070 1075 1080

Glu Thr Asp Phe Asp Ile Asp Tyr Ser Lys Val Ala Glu Met Asn

1085 1090 1095

Pro Glu Asn Val Asp Ile Asp Phe Arg Gly Asn Gln Thr Asp Trp

1100 1105 1110

Arg Lys Gly Arg Phe Ile Val Leu Thr Val His Asp Thr Tyr Asp

1115 1120 1125

Asp Asp Asp Asp Glu Lys Gly Ala Thr Ile Lys Glu Phe Ala Leu

1130 1135 1140

Phe Pro

1145

<210> 9

<211> 9187

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<400> 9

aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc 60

tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc 120

ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc 180

gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa 240

tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt 300

ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc 360

cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc 420

cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt 480

aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt 540

cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg ttaagaatac 600

tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg 660

ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa 720

ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag cgtggtgcac 780

tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 840

cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 900

cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 960

aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 1020

ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt 1080

gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat 1140

aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt 1200

caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata 1260

tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta 1320

cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag 1380

gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt 1440

tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt 1500

aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa 1560

atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 1620

tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 1680

aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 1740

acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 1800

acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 1860

ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 1920

ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 1980

caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 2040

ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 2100

aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt gatcgttggg 2160

aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 2220

tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 2280

aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 2340

cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 2400

ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggca 2460

gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 2520

agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 2580

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 2640

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2700

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2760

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2820

tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 2880

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 2940

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3000

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3060

cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag 3120

ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3180

agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3240

ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa aacgccagca 3300

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 3360

cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 3420

gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 3480

tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 3540

ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac ctcactcatt 3600

aggcacccca ggctttacac tttatgcttc cggctcctat gttgtgtgga attgtgagcg 3660

gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc ggaattaacc 3720

ctcactaaag ggaacaaaag ctgggtaccg ggccccccct cgagatctcc cgagtttatc 3780

attatcaata ctgccatttc aaagaatacg taaataatta atagtagtga ttttcctaac 3840

tttatttagt caaaaaattg gccttttaat tctgctgtaa cccgtacatg cccaaaatag 3900

ggggcgggtt acacagaata tataacatca taggtgtctg ggtgaacagt ttattcctgg 3960

catccactaa atataatgga gcccgctttt tttaagctgg catccagaaa aaaaaagaat 4020

cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc 4080

gcaactacac agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga 4140

tgcaacctgc ttggagtaaa tgatgacaca aggcaattga cctacgcatg tatctatctc 4200

attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa 4260

ggttgaaacc agttccctga aattattccc ctatttgact aataagtata taaagacggt 4320

aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag 4380

ttagtctttt ttttagtttt aaaacactaa gaacttagtt tcgaataaac acacataaaa 4440

acaaatctag aatgcttatc gtgccattca ttctcttcag cgtagcaatt ctagctccaa 4500

tatactttta cttgaccaaa cctttaccat tacaacatac tcattttggc tcttttgtct 4560

gcgctgagga taccttattc tgccctgaat ctcagagaaa agcctctgaa aagatgtata 4620

gtttattgaa ggatcacgaa aacacattct acgatgagga acagcaaatc ctgggaaact 4680

tattgttatc agaaaataca ttctcaagac aaccatatgt tgcaaacggt tacattggat 4740

ccagaatccc taaccttggg ttcggatacg cacttgatac cattaacgtt tgggtaaatg 4800

actcctctat accaggcgcc cttgataatg gttggccatt gagaaaccag agatttgctg 4860

gggcattcat ttcagacttc tactgtctac aagagaagct caactcaact aacttccctg 4920

aattggacga tgatggttac tcaactgtta tatcaacaat accacaatgg acagatctct 4980

caattttaaa gcacactact acaggtcaaa tcgagtacat taatcctaca gatgttaagt 5040

tagataagat tacaaactat atgcaaaact tatcacttca agatggaatt gtaacaacta 5100

catttgttta cgacaaacaa ctattggtta ctacaaaggt agttgcacat agaaagatat 5160

accctctcgg agtcgttacc ttagaactat ctttgttcga caacaattct gatactagtg 5220

ctgacaacga aaacgaatac gtcgaattag agatctgtga ttcactaaac ttctctacat 5280

cccacagaac cgtacttaat gattacggtt acgatcaaaa caacgagggt attttcatgg 5340

tggtggagcc agaaaacgtc ccatattcta atgcatccct tttttcatat tttgatatcc 5400

caagtagaga tacattaaca ctttctaagt actccgattc aatcagtcaa tgtacaacac 5460

aaatcttaaa ggcaaattca acttttgtag ctcacaagta catcggtata attagctctg 5520

agtacgataa caaacaacct gatgataact ctaatggaac ttctaatgaa gcgcttaaaa 5580

tgtctaacct ggaacgtgca accagtattg tgcttgataa caaaggtaat tacgattctc 5640

tgattcaatc acataaaaat gcttggaaga gaatttacaa agatgctagc attgaaatac 5700

catccgatgg tttgctggag atgacagcaa aaagttctat ttaccattta ctagcaaata 5760

caagatctca taacgtgtct gaggacagag gtttgcctat aggcgtgtca ggattatctt 5820

ctgattctta cgggggtatg gttttctggg attctgactt gtggattcta ccagcactcc 5880

tgccattctt cccaaacgct gcaagacaga tcaataacta cagaaatgca agccttcacc 5940

aggccaagct aaacgcagaa aagtacggct acgatggcgc gctataccca tggacatctg 6000

gcagatacgc gaactgtact tcaactggtc catgtgtcga ctacgaatat catatcaacg 6060

tggacatcgc tttgtcatct ttcgccatct acatgaatgg tgatgaagat gacgaacgtt 6120

ccgaagagta cttgcgttac acaacatggc cattcgtaga aaacgcagca aagatgtttg 6180

ctcaatacgt taagtggaat gacacaatgc aacaatacac cactcataac ttgactgatc 6240

cagatgaata tgcaaacttc gttgacaacg ctgcatttac taatgctggt atacaatctg 6300

tgatggtctg ggctcatgat atcgccagac acttgggcat cgatccagat ccacagtggt 6360

tggagatagc cgacaacatc catattccaa tttccgagac aaacatcaca ttggaatact 6420

caggtatgaa ctcctctgtc gaaatcaagc aagctgatgt ggtattgatg atttacccat 6480

tatcttacat aacagatcaa agcatcttga acaacgcaat taaaaacttg tactactatt 6540

ctgaacgtca aagtgcatca gggcctgcta tgacataccc agtatttgtc gccggagcgg 6600

catctcttct taactacggt tcatcttcac aatcctatct atacaagtca gttgtcccat 6660

acttacgtag tccttttgct caattctcag aacaaagtga cgacaacttc ttgaccaatg 6720

gtctaacaca gccagcgttc ccatttctaa cagctaacgg tggtttcttg cagtctattc 6780

tattcggttt gactgggctc agatacagtt acgaagttga tgaagataca gggaaaatcc 6840

atagattact gaagttcaat ccaattcagt tgcctctcct acctggaggt attagaatta 6900

acaactttaa gtacatgggt caggtattag atgttttgat cacagacacc gagggtatca 6960

tcatccacaa aaacggtact aaggaaatca gaatcaaagt tccagataga actctaattc 7020

ctgatgtaga tgtcaagtac tcagaagaga ctgatcctat taagcaaatt ttgcatggta 7080

gaagatctgt tccaaccggt aaaaactact tcactataca acctggtgat gtgttcaaga 7140

caccacttta catccctaaa aaaaacttgg aaggcaattt ggtcgaggct aagcaaatta 7200

ccaatttgac tgccggcgtc ccaggtgacg ttgctgtctc tgtaatagat ggaaacaact 7260

ttacacactg gcaaccagca tacaaaaacc ttccagccag attgttaatc gatatgggca 7320

ataattttac ccaagagatt aagtccggca agatcatatg gggctcaaga ccagcaaaaa 7380

cattctccct gtcaattttg ccacagacag aagaggtttt caaaaatctt acacatattt 7440

tatctaacgt taatcaatat tgtaacaaga caggtgatga gtgcgtcaga gatttagaga 7500

gaacagagaa agggttcgat gccgcaatag aagatgtttt cgactggtac ggtattgacc 7560

cagacagcat tatctcaaca catcctgaat taaaagatat gaaaacaaag ttcgtcaaga 7620

tcctagatca ctacaaagtc actccatccg aaccttatcc atggagagtt tacaacgagt 7680

ctcaaatcgt gctgcttcca ggcaatgaaa cagatttcga tattgattac tctaaagttg 7740

ctgaaatgaa ccctgaaaac gttgatattg atttcagagg gaaccagaca gattggagaa 7800

agggaagatt tatcgttctc acagtgcatg atacttacga cgacgatgat gacgaaaagg 7860

gtgccactat caaggaattt gcattattcc cttgattaat taaacaggcc ccttttcctt 7920

tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcctc ccacatccgc 7980

tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat 8040

agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacaa 8100

acgcgtgtac gcatgtaacg ggcagacgcg gccgccaccg cggtggagct ccaattcgcc 8160

ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa 8220

ccctggcgtt acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa 8280

tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 8340

gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 8400

gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 8460

cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 8520

atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 8580

tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 8640

tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 8700

tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 8760

atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg gtattttctc 8820

cttacgcatc tgtgcggtat ttcacaccgc agggtaataa ctgatataat taaattgaag 8880

ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct 8940

ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct 9000

tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag 9060

agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac 9120

ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt 9180

gagcaat 9187

<210> 10

<211> 8866

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体近平滑假丝酵母和酿酒酵母共同组成的序列

<400> 10

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 60

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 120

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 180

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 240

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 300

aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 360

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 420

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 480

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttt 540

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 600

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 660

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 720

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 780

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 840

ggtaagccct cccgtatcgt agttatctac acgacgggca gtcaggcaac tatggatgaa 900

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 960

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 1020

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 1080

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 1140

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 1200

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 1260

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 1320

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 1380

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 1440

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 1500

ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 1560

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaacgcc 1620

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 1680

tgctcgtcag gggggccgag cctatggaaa aacgccagca acgcggcctt tttacggttc 1740

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 1800

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 1860

cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 1920

gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 1980

agtgagcgca acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac 2040

tttatgcttc cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga 2100

aacagctatg accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag 2160

ctgggtaccg ggccccccct cgagatctcc cgagtttatc attatcaata ctgccatttc 2220

aaagaatacg taaataatta atagtagtga ttttcctaac tttatttagt caaaaaattg 2280

gccttttaat tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata 2340

tataacatca taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga 2400

gcccgctttt tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt 2460

ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacac agaacagggg 2520

cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ttggagtaaa 2580

tgatgacaca aggcaattga cctacgcatg tatctatctc attttcttac accttctatt 2640

accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga 2700

aattattccc ctatttgact aataagtata taaagacggt aggtattgat tgtaattctg 2760

taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt 2820

aaaacactaa gaacttagtt tcgaataaac acacataaaa acaaatctag aatgaaattc 2880

atcagtacct ttttaacatt tatcttagct gcagtctcag ttacagcgag attttctgag 2940

gcgttccctt tcgtcttgcc aagagcagaa aagtacacat tggaacaaac atcagattct 3000

aacttgggcg atttcgtcaa ctcattcgag aacaaggaga tcttttcaca agttcagttc 3060

tcagagcatg ctttctacga tagaaaaatg aacgtagtgg ggactattga atactctaag 3120

tacaaccaat ttcagaaaca accatatgta gctaacggtt acattggatc cagaatccca 3180

aacttgggtc atggtttcac atacgatcaa ttaactgagt ctccagattc caaaaaagat 3240

gacttgtata acggctggcc tctgttcgat aaaagattct caggtgcctt tatcgctgga 3300

ttttacgatt tacaggaaaa tactaccgga aataacttcc cagaactttt aaaaaacggt 3360

tacgaatccg ttattagtgc catccctcaa tggactactc tcaagctcgc aatccaacac 3420

ggcgataaga gatacacttt ggaccctgca agtccagatg gtgttggtca aataactaac 3480

tacgtccaaa atatgtcctt aagcactggc attgttacaa cacaatttac atggttagaa 3540

tcactagatg taagatatac agtgcttgct catcgttccg agattaactt gggcctagtt 3600

aaggtcgata ttaaaaacaa gggaaatgat acagttgaaa ttgatgtaat cgatgagtta 3660

aacttcgata ctgctcagag atgctctctg aatttgatcg ggcatgataa ttctggtatt 3720

tacgtgtctt tctccccaga taatctggat tacatttctg gagctatcta cagcacacta 3780

cacctcgatt ctgtgaagag acattctaaa aacaaaaaga caaaccagac ctcaagcata 3840

cgtctgcatg ctcaagacag tacacagttt ggaaagactg taggtatagt ttcaaccgac 3900

ctggacccat ctaagttcaa aaacagtcgt gacactttgg aatttgcaaa aaaagtgtcc 3960

caaaagttcg aatacttgga cttagttatt aagagtcatg tcagagcgtg gaaggaacta 4020

atgggcaagc aaagtacaat aacttttcca aatgatccat tgcttgattt gggatctaag 4080

gcttcagttt atcacttgct agctaacacc agaccagatg cacaaggcct aactggtgct 4140

ctcggcgtcg ctggcctttc ttctgattca tacggtggca tggtgttctg ggacaccgat 4200

ttctggatgt tcagagcgat actggcccta gatcctacac acgcaaagtc aatagtcaac 4260

tacagaatgc atacacacca acaagcactc aaaaacacac ctcagggtta cgaaggggcc 4320

gcttaccctt ggacttctgg tcgttttggc aactgcacag ccaccgggcc ttgtttaaat 4380

tacgaatatc acattaactc agccatagca atggctgctt gggaattgta catcagtgga 4440

gcagtagacg atcaatactt agagagtaca gtctacccat tgatttcaga cgctgcaaag 4500

ttctacgccg attacgtaac atcatataac gaaactatcg acaagttcat tacatcaaac 4560

ctaaccgacc cagatgaata cgctaatcac gtagacaatg gtgcctatac aaatgccggt 4620

attgcccttc ttatgaattg gatcaatgac atcggcgaca tcctggggat taagatgcca 4680

gaaatttaca ctgatatatc tcaaaagatg tatctcccaa ctgcggataa ttctcaaaac 4740

attaccttag aatactctgg tatgaactcc tctgtcggaa tcaaacaagc tgatgttgtc 4800

cttatgacat acccacttca gaactctcta gttgacgatg aacaggcata cattaacatg 4860

gagttttact caggaaaaca agtttcttac ggaccagcaa tgaccttctc tatattttct 4920

attgtcgcag caaacttggc tacatctggt tgtgcctcac aatcttactt gcacaaagca 4980

atgcaaccat atcttagagg ccctttcgca caattctccg aacaaaataa tgacaacttc 5040

aagacaaacg gtggaacaca tccagcttat ccatttctta ctgcacatgg tgggttcata 5100

caggctgttg tgaacggact tactgggctt agacattcct acacaatcga taatggtaag 5160

attcgtagat cactattctt agatcctatc gccttacctt gccttggcga tggggttcaa 5220

tactccggta ttcattacga taatcataca attagcatga acattacagg ttccaccttc 5280

gttatcagaa acgagggcaa aaccaacaag agagcaaacg actatatctc tatcactatt 5340

gccgatagaa atccaggtag aggtacatac aagttgaagg acaatgaaga gaaaagcttc 5400

aagttatacc aaccttctaa gatttttgaa gattctgtgt cagagtgtgg tttggcaact 5460

ttctacaata taacagaatc agctcctggt gacagctctt tctccataaa cgatggtgat 5520

aacactacca gatggcaagt taagtacaat gatacaacag gtaaggtctt gatcgatttg 5580

cattcaatca aaaacatcac tggtgttgtt ttcaactggg ctgataagcc acctaaaaag 5640

gtaaagttga gtaagtacat ggggactaag tttaaggatg tcactgactt tttggctaag 5700

gttgatttcg gtaattacgt ttacaaaaac tacagattcg ctaaccctgg tgaaaaactt 5760

ttgaaccaat cagaagtgtt cgaaaacgtt tactctgatg atgttacaat caacgcacca 5820

ttctcttcta aggaactaga ggaagttatc gttccagtga gacataatac tacatcttgg 5880

aacattaact tacaaggcag attcttattg ttagaagtgg acggaatcca caacaccatt 5940

ccaatcgaag atgattacgg tggcgcaaag ttagccgaag tgaacttctt ttagttaatt 6000

aaacaggccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac 6060

gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg 6120

tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt 6180

ttcttttttt tctgtacaaa cgcgtgtacg catgtaacgg gcagacgcgg ccgccaccgc 6240

ggtggagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac 6300

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 6360

ccttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 6420

gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg 6480

tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 6540

ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 6600

ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 6660

agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 6720

tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 6780

tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 6840

atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 6900

cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca gggtaataac 6960

tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat 7020

acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg 7080

taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata 7140

ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg 7200

tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct 7260

cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca 7320

atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca tgacaattct 7380

gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg 7440

ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt 7500

ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg 7560

tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc 7620

tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct 7680

aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag 7740

tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta 7800

gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt 7860

gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg 7920

cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt 7980

accgaatcaa aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat 8040

gaattgaatt gaaaagcgtg gtgcactctc agtacaatct gctctgatgc cgcatagtta 8100

agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 8160

gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 8220

ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt 8280

aatgtcatga taataatggt ttcttaggac ggatcgcttg cctgtaactt acacgcgcct 8340

cgtatctttt aatgatggaa taatttggga atttactctg tgtttattta tttttatgtt 8400

ttgtatttgg attttagaaa gtaaataaag aaggtagaag agttacggaa tgaagaaaaa 8460

aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta ctttacatat atatttatta 8520

gacaagaaaa gcagattaaa tagatataca ttcgattaac gataagtaaa atgtaaaatc 8580

acaggatttt cgtgtgtggt cttctacaca gacaagatga aacaattcgg cattaatacc 8640

tgagagcagg aagagcaaga taaaaggtag tatttgttgg cgatccccct agagtctttt 8700

acatcttcgg aaaacaaaaa ctattttttc tttaatttct ttttttactt tctattttta 8760

atttatatat ttatattaaa aaatttaaat tataattatt tttatagcac gtgatgaaaa 8820

ggacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatt 8866

<210> 11

<211> 1040

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<400> 11

Met Lys Phe Ile Ser Thr Phe Leu Thr Phe Ile Leu Ala Ala Val Ser

1 5 10 15

Val Thr Ala Arg Phe Ser Glu Ala Phe Pro Phe Val Leu Pro Arg Ala

20 25 30

Glu Lys Tyr Thr Leu Glu Gln Thr Ser Asp Ser Asn Leu Gly Asp Phe

35 40 45

Val Asn Ser Phe Glu Asn Lys Glu Ile Phe Ser Gln Val Gln Phe Ser

50 55 60

Glu His Ala Phe Tyr Asp Arg Lys Met Asn Val Val Gly Thr Ile Glu

65 70 75 80

Tyr Ser Lys Tyr Asn Gln Phe Gln Lys Gln Pro Tyr Val Ala Asn Gly

85 90 95

Tyr Ile Gly Ser Arg Ile Pro Asn Leu Gly His Gly Phe Thr Tyr Asp

100 105 110

Gln Leu Thr Glu Ser Pro Asp Ser Lys Lys Asp Asp Leu Tyr Asn Gly

115 120 125

Trp Pro Leu Phe Asp Lys Arg Phe Ser Gly Ala Phe Ile Ala Gly Phe

130 135 140

Tyr Asp Leu Gln Glu Asn Thr Thr Gly Asn Asn Phe Pro Glu Leu Leu

145 150 155 160

Lys Asn Gly Tyr Glu Ser Val Ile Ser Ala Ile Pro Gln Trp Thr Thr

165 170 175

Leu Lys Leu Ala Ile Gln His Gly Asp Lys Arg Tyr Thr Leu Asp Pro

180 185 190

Ala Ser Pro Asp Gly Val Gly Gln Ile Thr Asn Tyr Val Gln Asn Met

195 200 205

Ser Leu Ser Thr Gly Ile Val Thr Thr Gln Phe Thr Trp Leu Glu Ser

210 215 220

Leu Asp Val Arg Tyr Thr Val Leu Ala His Arg Ser Glu Ile Asn Leu

225 230 235 240

Gly Leu Val Lys Val Asp Ile Lys Asn Lys Gly Asn Asp Thr Val Glu

245 250 255

Ile Asp Val Ile Asp Glu Leu Asn Phe Asp Thr Ala Gln Arg Cys Ser

260 265 270

Leu Asn Leu Ile Gly His Asp Asn Ser Gly Ile Tyr Val Ser Phe Ser

275 280 285

Pro Asp Asn Leu Asp Tyr Ile Ser Gly Ala Ile Tyr Ser Thr Leu His

290 295 300

Leu Asp Ser Val Lys Arg His Ser Lys Asn Lys Lys Thr Asn Gln Thr

305 310 315 320

Ser Ser Ile Arg Leu His Ala Gln Asp Ser Thr Gln Phe Gly Lys Thr

325 330 335

Val Gly Ile Val Ser Thr Asp Leu Asp Pro Ser Lys Phe Lys Asn Ser

340 345 350

Arg Asp Thr Leu Glu Phe Ala Lys Lys Val Ser Gln Lys Phe Glu Tyr

355 360 365

Leu Asp Leu Val Ile Lys Ser His Val Arg Ala Trp Lys Glu Leu Met

370 375 380

Gly Lys Gln Ser Thr Ile Thr Phe Pro Asn Asp Pro Leu Leu Asp Leu

385 390 395 400

Gly Ser Lys Ala Ser Val Tyr His Leu Leu Ala Asn Thr Arg Pro Asp

405 410 415

Ala Gln Gly Leu Thr Gly Ala Leu Gly Val Ala Gly Leu Ser Ser Asp

420 425 430

Ser Tyr Gly Gly Met Val Phe Trp Asp Thr Asp Phe Trp Met Phe Arg

435 440 445

Ala Ile Leu Ala Leu Asp Pro Thr His Ala Lys Ser Ile Val Asn Tyr

450 455 460

Arg Met His Thr His Gln Gln Ala Leu Lys Asn Thr Pro Gln Gly Tyr

465 470 475 480

Glu Gly Ala Ala Tyr Pro Trp Thr Ser Gly Arg Phe Gly Asn Cys Thr

485 490 495

Ala Thr Gly Pro Cys Leu Asn Tyr Glu Tyr His Ile Asn Ser Ala Ile

500 505 510

Ala Met Ala Ala Trp Glu Leu Tyr Ile Ser Gly Ala Val Asp Asp Gln

515 520 525

Tyr Leu Glu Ser Thr Val Tyr Pro Leu Ile Ser Asp Ala Ala Lys Phe

530 535 540

Tyr Ala Asp Tyr Val Thr Ser Tyr Asn Glu Thr Ile Asp Lys Phe Ile

545 550 555 560

Thr Ser Asn Leu Thr Asp Pro Asp Glu Tyr Ala Asn His Val Asp Asn

565 570 575

Gly Ala Tyr Thr Asn Ala Gly Ile Ala Leu Leu Met Asn Trp Ile Asn

580 585 590

Asp Ile Gly Asp Ile Leu Gly Ile Lys Met Pro Glu Ile Tyr Thr Asp

595 600 605

Ile Ser Gln Lys Met Tyr Leu Pro Thr Ala Asp Asn Ser Gln Asn Ile

610 615 620

Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Gly Ile Lys Gln Ala

625 630 635 640

Asp Val Val Leu Met Thr Tyr Pro Leu Gln Asn Ser Leu Val Asp Asp

645 650 655

Glu Gln Ala Tyr Ile Asn Met Glu Phe Tyr Ser Gly Lys Gln Val Ser

660 665 670

Tyr Gly Pro Ala Met Thr Phe Ser Ile Phe Ser Ile Val Ala Ala Asn

675 680 685

Leu Ala Thr Ser Gly Cys Ala Ser Gln Ser Tyr Leu His Lys Ala Met

690 695 700

Gln Pro Tyr Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Asn Asn

705 710 715 720

Asp Asn Phe Lys Thr Asn Gly Gly Thr His Pro Ala Tyr Pro Phe Leu

725 730 735

Thr Ala His Gly Gly Phe Ile Gln Ala Val Val Asn Gly Leu Thr Gly

740 745 750

Leu Arg His Ser Tyr Thr Ile Asp Asn Gly Lys Ile Arg Arg Ser Leu

755 760 765

Phe Leu Asp Pro Ile Ala Leu Pro Cys Leu Gly Asp Gly Val Gln Tyr

770 775 780

Ser Gly Ile His Tyr Asp Asn His Thr Ile Ser Met Asn Ile Thr Gly

785 790 795 800

Ser Thr Phe Val Ile Arg Asn Glu Gly Lys Thr Asn Lys Arg Ala Asn

805 810 815

Asp Tyr Ile Ser Ile Thr Ile Ala Asp Arg Asn Pro Gly Arg Gly Thr

820 825 830

Tyr Lys Leu Lys Asp Asn Glu Glu Lys Ser Phe Lys Leu Tyr Gln Pro

835 840 845

Ser Lys Ile Phe Glu Asp Ser Val Ser Glu Cys Gly Leu Ala Thr Phe

850 855 860

Tyr Asn Ile Thr Glu Ser Ala Pro Gly Asp Ser Ser Phe Ser Ile Asn

865 870 875 880

Asp Gly Asp Asn Thr Thr Arg Trp Gln Val Lys Tyr Asn Asp Thr Thr

885 890 895

Gly Lys Val Leu Ile Asp Leu His Ser Ile Lys Asn Ile Thr Gly Val

900 905 910

Val Phe Asn Trp Ala Asp Lys Pro Pro Lys Lys Val Lys Leu Ser Lys

915 920 925

Tyr Met Gly Thr Lys Phe Lys Asp Val Thr Asp Phe Leu Ala Lys Val

930 935 940

Asp Phe Gly Asn Tyr Val Tyr Lys Asn Tyr Arg Phe Ala Asn Pro Gly

945 950 955 960

Glu Lys Leu Leu Asn Gln Ser Glu Val Phe Glu Asn Val Tyr Ser Asp

965 970 975

Asp Val Thr Ile Asn Ala Pro Phe Ser Ser Lys Glu Leu Glu Glu Val

980 985 990

Ile Val Pro Val Arg His Asn Thr Thr Ser Trp Asn Ile Asn Leu Gln

995 1000 1005

Gly Arg Phe Leu Leu Leu Glu Val Asp Gly Ile His Asn Thr Ile

1010 1015 1020

Pro Ile Glu Asp Asp Tyr Gly Gly Ala Lys Leu Ala Glu Val Asn

1025 1030 1035

Phe Phe

1040

<210> 12

<211> 8959

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<400> 12

tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc 60

cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag 120

taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa 180

atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac 240

taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt 300

ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc 360

cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag 420

ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac 480

caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa 540

aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg 600

aaaagcgtgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 660

acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 720

cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 780

gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 840

aataatggtt tcttaggacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta 900

atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga 960

ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa 1020

aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag 1080

cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc 1140

gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga 1200

agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga 1260

aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt 1320

tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg 1380

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 1440

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 1500

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1560

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1620

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1680

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1740

tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1800

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1860

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1920

cgatcggagg accgaaggag ctaaccgctt tttttcacaa catgggggat catgtaactc 1980

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 2040

cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 2100

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 2160

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 2220

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 2280

tctacacgac gggcagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 2340

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 2400

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 2460

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 2520

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2580

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2640

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 2700

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2760

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2820

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2880

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg 2940

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 3000

gagagcgcac gagggagctt ccagggggga acgcctggta tctttatagt cctgtcgggt 3060

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg ccgagcctat 3120

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 3180

acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 3240

gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 3300

cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 3360

gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 3420

gttacctcac tcattaggca ccccaggctt tacactttat gcttccggct cctatgttgt 3480

gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 3540

agctcggaat taaccctcac taaagggaac aaaagctggg taccgggccc cccctcgaga 3600

tctcccgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt 3660

agtgattttc ctaactttat ttagtcaaaa aattggcctt ttaattctgc tgtaacccgt 3720

acatgcccaa aatagggggc gggttacaca gaatatataa catcataggt gtctgggtga 3780

acagtttatt cctggcatcc actaaatata atggagcccg ctttttttaa gctggcatcc 3840

agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag 3900

gtccattctc ttagcgcaac tacacagaac aggggcacaa acaggcaaaa aacgggcaca 3960

acctcaatgg agtgatgcaa cctgcttgga gtaaatgatg acacaaggca attgacctac 4020

gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa 4080

aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctatt tgactaataa 4140

gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa 4200

attctacttt tatagttagt ctttttttta gttttaaaac actaagaact tagtttcgaa 4260

taaacacaca taaaaacaaa tctagaatgg acatcgaaaa caatggtatt tacatggaaa 4320

atagcagtag aatcaagggt atacgtagat tattcaaaga caacaagaca tatatcattg 4380

ccatgactat catagcaaat ttcattctgt tttactctct acacttactt aataccagat 4440

tttctgaggc gttccctttc gtcttgccaa gagcagaaaa gtacacattg gaacaaacat 4500

cagattctaa cttgggcgat ttcgtcaact cattcgagaa caaggagatc ttttcacaag 4560

ttcagttctc agagcatgct ttctacgata gaaaaatgaa cgtagtgggg actattgaat 4620

actctaagta caaccaattt cagaaacaac catatgtagc taacggttac attggatcca 4680

gaatcccaaa cttgggtcat ggtttcacat acgatcaatt aactgagtct ccagattcca 4740

aaaaagatga cttgtataac ggctggcctc tgttcgataa aagattctca ggtgccttta 4800

tcgctggatt ttacgattta caggaaaata ctaccggaaa taacttccca gaacttttaa 4860

aaaacggtta cgaatccgtt attagtgcca tccctcaatg gactactctc aagctcgcaa 4920

tccaacacgg cgataagaga tacactttgg accctgcaag tccagatggt gttggtcaaa 4980

taactaacta cgtccaaaat atgtccttaa gcactggcat tgttacaaca caatttacat 5040

ggttagaatc actagatgta agatatacag tgcttgctca tcgttccgag attaacttgg 5100

gcctagttaa ggtcgatatt aaaaacaagg gaaatgatac agttgaaatt gatgtaatcg 5160

atgagttaaa cttcgatact gctcagagat gctctctgaa tttgatcggg catgataatt 5220

ctggtattta cgtgtctttc tccccagata atctggatta catttctgga gctatctaca 5280

gcacactaca cctcgattct gtgaagagac attctaaaaa caaaaagaca aaccagacct 5340

caagcatacg tctgcatgct caagacagta cacagtttgg aaagactgta ggtatagttt 5400

caaccgacct ggacccatct aagttcaaaa acagtcgtga cactttggaa tttgcaaaaa 5460

aagtgtccca aaagttcgaa tacttggact tagttattaa gagtcatgtc agagcgtgga 5520

aggaactaat gggcaagcaa agtacaataa cttttccaaa tgatccattg cttgatttgg 5580

gatctaaggc ttcagtttat cacttgctag ctaacaccag accagatgca caaggcctaa 5640

ctggtgctct cggcgtcgct ggcctttctt ctgattcata cggtggcatg gtgttctggg 5700

acaccgattt ctggatgttc agagcgatac tggccctaga tcctacacac gcaaagtcaa 5760

tagtcaacta cagaatgcat acacaccaac aagcactcaa aaacacacct cagggttacg 5820

aaggggccgc ttacccttgg acttctggtc gttttggcaa ctgcacagcc accgggcctt 5880

gtttaaatta cgaatatcac attaactcag ccatagcaat ggctgcttgg gaattgtaca 5940

tcagtggagc agtagacgat caatacttag agagtacagt ctacccattg atttcagacg 6000

ctgcaaagtt ctacgccgat tacgtaacat catataacga aactatcgac aagttcatta 6060

catcaaacct aaccgaccca gatgaatacg ctaatcacgt agacaatggt gcctatacaa 6120

atgccggtat tgcccttctt atgaattgga tcaatgacat cggcgacatc ctggggatta 6180

agatgccaga aatttacact gatatatctc aaaagatgta tctcccaact gcggataatt 6240

ctcaaaacat taccttagaa tactctggta tgaactcctc tgtcggaatc aaacaagctg 6300

atgttgtcct tatgacatac ccacttcaga actctctagt tgacgatgaa caggcataca 6360

ttaacatgga gttttactca ggaaaacaag tttcttacgg accagcaatg accttctcta 6420

tattttctat tgtcgcagca aacttggcta catctggttg tgcctcacaa tcttacttgc 6480

acaaagcaat gcaaccatat cttagaggcc ctttcgcaca attctccgaa caaaataatg 6540

acaacttcaa gacaaacggt ggaacacatc cagcttatcc atttcttact gcacatggtg 6600

ggttcataca ggctgttgtg aacggactta ctgggcttag acattcctac acaatcgata 6660

atggtaagat tcgtagatca ctattcttag atcctatcgc cttaccttgc cttggcgatg 6720

gggttcaata ctccggtatt cattacgata atcatacaat tagcatgaac attacaggtt 6780

ccaccttcgt tatcagaaac gagggcaaaa ccaacaagag agcaaacgac tatatctcta 6840

tcactattgc cgatagaaat ccaggtagag gtacatacaa gttgaaggac aatgaagaga 6900

aaagcttcaa gttataccaa ccttctaaga tttttgaaga ttctgtgtca gagtgtggtt 6960

tggcaacttt ctacaatata acagaatcag ctcctggtga cagctctttc tccataaacg 7020

atggtgataa cactaccaga tggcaagtta agtacaatga tacaacaggt aaggtcttga 7080

tcgatttgca ttcaatcaaa aacatcactg gtgttgtttt caactgggct gataagccac 7140

ctaaaaaggt aaagttgagt aagtacatgg ggactaagtt taaggatgtc actgactttt 7200

tggctaaggt tgatttcggt aattacgttt acaaaaacta cagattcgct aaccctggtg 7260

aaaaactttt gaaccaatca gaagtgttcg aaaacgttta ctctgatgat gttacaatca 7320

acgcaccatt ctcttctaag gaactagagg aagttatcgt tccagtgaga cataatacta 7380

catcttggaa cattaactta caaggcagat tcttattgtt agaagtggac ggaatccaca 7440

acaccattcc aatcgaagat gattacggtg gcgcaaagtt agccgaagtg aacttctttt 7500

agttaattaa acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt 7560

acattcacgc cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga 7620

agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt 7680

tcaaattttt cttttttttc tgtacaaacg cgtgtacgca tgtaacgggc agacgcggcc 7740

gccaccgcgg tggagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt 7800

cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 7860

acatcccccc ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 7920

acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc 7980

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 8040

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 8100

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 8160

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 8220

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 8280

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 8340

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 8400

tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcagg 8460

gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta 8520

cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg 8580

cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc 8640

caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac 8700

ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac 8760

cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc 8820

tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg 8880

acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct 8940

tcaaaccgct aacaatacc 8959

<210> 13

<211> 9130

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体光滑假丝酵母和酿酒酵母共同组成的序列

<400> 13

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 60

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 120

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 180

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 240

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 300

aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 360

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 420

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 480

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttt 540

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 600

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 660

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 720

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 780

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 840

ggtaagccct cccgtatcgt agttatctac acgacgggca gtcaggcaac tatggatgaa 900

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 960

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 1020

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 1080

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 1140

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 1200

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 1260

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 1320

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 1380

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 1440

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 1500

ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 1560

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaacgcc 1620

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 1680

tgctcgtcag gggggccgag cctatggaaa aacgccagca acgcggcctt tttacggttc 1740

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 1800

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 1860

cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 1920

gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 1980

agtgagcgca acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac 2040

tttatgcttc cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga 2100

aacagctatg accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag 2160

ctgggtaccg ggccccccct cgagatctcc cgagtttatc attatcaata ctgccatttc 2220

aaagaatacg taaataatta atagtagtga ttttcctaac tttatttagt caaaaaattg 2280

gccttttaat tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata 2340

tataacatca taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga 2400

gcccgctttt tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt 2460

ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacac agaacagggg 2520

cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ttggagtaaa 2580

tgatgacaca aggcaattga cctacgcatg tatctatctc attttcttac accttctatt 2640

accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga 2700

aattattccc ctatttgact aataagtata taaagacggt aggtattgat tgtaattctg 2760

taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt 2820

aaaacactaa gaacttagtt tcgaataaac acacataaaa acaaatctag aatgaaattc 2880

atctctactt tccttacttt tattttagct gccgtatctg taaccgcctc taccaactct 2940

tacttctctt cctccttagc ttctgcttca ccattattca acaaagaggg cagagtggtt 3000

agaccaatgc gtgaaagtaa cttaggccta cacgcagatc ctcaaactag aaaatccagt 3060

aagacacttt acgatttgtt gtcagacttt gacaatgcat tttacgatga tgaaaacatg 3120

atcttaggat ccctagcctt cggtgagaat acctactcca gacagccata cgtcgcaaac 3180

gggtacatag gtagccgtat accaaacatc ggtttcggat acgcacttga tacacttaac 3240

ttgtacgctg atgcaccagg cgcacttaac aacggttggc ctttgagaaa cagaagattc 3300

gctggaagtt tcgtttctga tttctactca ctgcaagcca agttgaacag taccaatttt 3360

cctgagttag atgaaaaggg ttatacaact gtcatttcat caatcccaga atggacagat 3420

ttgcaattca ctgtagacct taacggcact aactggttca atccacaatc agttctaatc 3480

gatgatgtta ttaattacaa tcagaacttg agcatgaagg atggaattgt tagcacaaac 3540

atggattggt tgaacggaat gataaacatt aagtctgaag tctgggcaca cagaaagatc 3600

cattccttgg gtatcacacg tctggaaatc tcattgaacc ttgatgcttt acctgacgag 3660

ttcactgaac taccagttac aatttacgat atcatagatt tcaacacttc tcatagaaca 3720

acactatatg agaaaggtca agatgaagat aacaaagcga tttacatgat tgtaaaccca 3780

gaaaacgtac catactcaaa cgcagttgtg tactctactt gtacaatcaa gggcactgaa 3840

aacaactttt ctccttacaa cttcacatca gatgatagaa ttgcgagaaa ctacatgaca 3900

aacctaacag aagagaaccc aaaagtcgtt atttacaagt acacatcagt ggtttcctca 3960

gaatataata atgacgagcc aaatcctaac gttaacttga agttcgcctc aaacatcgct 4020

aacaccgcaa aaggaaacta taagtcttta ttgtccaatc acaaaagagc ttggtacgat 4080

ttgtacaatg atgcctttat cgaaattcca tctgatagtt tattggaaat gaccgcaaga 4140

tctagtctct tccatttgct tgcgaacaca cgtcaataca acgtttctac aactagagga 4200

ttaccagtcg gcgttggtgg tctttcttca gactcttacg gggggatggt cttttgggac 4260

gcagacgtat ggatggctcc agctctccta cctttctttc caaacatagc aatgaacatg 4320

aataactaca gaaacgctac tcatcaacaa gccattgaaa atgctaagca atacaattac 4380

ccaggcgccg tttacccttg gacatctggt agatatgcga attgtacctc taccggtcct 4440

tgcatcgact acgaatacca tattaatgta gatatcgctt tggcaagttt ctctatttac 4500

atgaacggtg ccgagggtgc cgacgaagat taccttagat tcacaacatg gccaatggtc 4560

aaggatgctg cagtgttctt caaggcctac gtcaaataca atgaaacact tggagagtac 4620

gaaacttata atttaactga cccagatgaa tttgctaatc atgtgaataa tggagctttc 4680

acaaatgcag gaatcaaaac tttgttgaaa tgggcaacag acattggtac tcaccttggg 4740

gaggaagttg atcctaagtg gatggaaatt gctgataaca tacatattcc tagaagtgac 4800

tctaacatta ctttggagta ctctggcatg aatagctccg ttgagattaa gcaggctgac 4860

gttacattga tggtataccc tttaggttac atcaacgatg aatctatact aaacaacgca 4920

atcaaggatt tgtattacta ctctgaacgt cagtctgcct ctgggccagc tatgacttac 4980

ccagtgttcg tcgcagctgc cgcttccctc ttgaaccacg gttcatcatc acagagttac 5040

ctttacaaat ctgttcttcc atatctgaga tcaccatttg ctcaatttag cgaacaatca 5100

gatgacaatt tcttaactaa cggtttgaca caaccagcat tcccattttt gaccgctaat 5160

ggtggtttct tgcaatctat attatttgga ttaaccggat tgagatacag ttacgaagtt 5220

actcctagaa ccaaaaagat ttcaagacta ctcaagtttg atccagtcaa gttgccactg 5280

ctacctggcg gaattgcaat tagaaatttc aaatacatgg gacaagtact ggatatcata 5340

atagacgata acaatggtac aatagctcat aagggtgggg ataagccaat tagaatcaag 5400

gttccaaata gagatattct ccacgacaga aatatcactt ccgcactata ctcaaagaga 5460

gatgacgatt tatcagcgac agatgactac tacggtacat acttcacact atacccaaat 5520

gaggaactgg tgattcctct ttacgatact aagttaaaca tcgacggtaa catagccgaa 5580

tctaaacaaa taactaattt gactgccgga gttccaggtg acgttggttt ctccgcatta 5640

gatgggaaca attacacaca ctggcagcca ttcgataaat cagataatgc taagttgcta 5700

attgacttag gttttaacag cactcatgtc attaaaaagg gtattatttt gtggggtcaa 5760

agaccagcga aaaacatttc tttgtcagtt ttgccacatt cagagagaat tgagcaacta 5820

tttgcgaaca tcacagatct tttagaaaca tcatctataa ctaagggcgg ctctttgtta 5880

aaccagatgt taggccaaac acaatcaaac gtgaccgcag aaattgatga tgatatcctc 5940

gctttactga actggaaagg tgacgatctg gaccaactca tcccttattt gcctgatatg 6000

catctcctac aagagaaatt cattcctatt ttgaaggatt accctattaa gccaaatcag 6060

agatactata aggaaatcat tgacgatgac ataatcaagt tattaccatc aaacacaact 6120

gaatttacaa tcgattacaa ttctatccct ggcggggaaa agcgtgcaag atacgttgtt 6180

ctgacagttc acggcaccta tgatgacgat gatgatctta aaggtgcaac aatcaaggaa 6240

attgttctgc aggaatgatt aattaaacag gccccttttc ctttgtcgat atcatgtaat 6300

tagttatgtc acgcttacat tcacgccctc ctcccacatc cgctctaacc gaaaaggaag 6360

gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa 6420

gaacgttatt tatatttcaa atttttcttt tttttctgta caaacgcgtg tacgcatgta 6480

acgggcagac gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt 6540

acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 6600

ttaatcgcct tgcagcacat ccccccttcg ccagctggcg taatagcgaa gaggcccgca 6660

ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta 6720

gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 6780

gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 6840

ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 6900

acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 6960

agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 7020

aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 7080

cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 7140

acaaaatatt aacgtttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg 7200

tatttcacac cgcagggtaa taactgatat aattaaattg aagctctaat ttgtgagttt 7260

agtatacatg catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa 7320

tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt 7380

gcaaatagtc ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg 7440

gttctatact gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata 7500

atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata 7560

acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat 7620

agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact 7680

tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc 7740

gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta 7800

ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat 7860

gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt 7920

tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta 7980

cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg 8040

gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt 8100

tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt 8160

catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt 8220

cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa ccgaaatcaa 8280

aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag cgtggtgcac tctcagtaca 8340

atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg 8400

ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 8460

agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc 8520

gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta ggacggatcg 8580

cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac 8640

tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta 8700

gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag 8760

cgtactttac atatatattt attagacaag aaaagcagat taaatagata tacattcgat 8820

taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag 8880

atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg 8940

ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat 9000

ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt aaattataat 9060

tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg 9120

aacccctatt 9130

<210> 14

<211> 1128

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体光滑假丝酵母和酿酒酵母共同组成的序列

<400> 14

Met Lys Phe Ile Ser Thr Phe Leu Thr Phe Ile Leu Ala Ala Val Ser

1 5 10 15

Val Thr Ala Ser Thr Asn Ser Tyr Phe Ser Ser Ser Leu Ala Ser Ala

20 25 30

Ser Pro Leu Phe Asn Lys Glu Gly Arg Val Val Arg Pro Met Arg Glu

35 40 45

Ser Asn Leu Gly Leu His Ala Asp Pro Gln Thr Arg Lys Ser Ser Lys

50 55 60

Thr Leu Tyr Asp Leu Leu Ser Asp Phe Asp Asn Ala Phe Tyr Asp Asp

65 70 75 80

Glu Asn Met Ile Leu Gly Ser Leu Ala Phe Gly Glu Asn Thr Tyr Ser

85 90 95

Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn

100 105 110

Ile Gly Phe Gly Tyr Ala Leu Asp Thr Leu Asn Leu Tyr Ala Asp Ala

115 120 125

Pro Gly Ala Leu Asn Asn Gly Trp Pro Leu Arg Asn Arg Arg Phe Ala

130 135 140

Gly Ser Phe Val Ser Asp Phe Tyr Ser Leu Gln Ala Lys Leu Asn Ser

145 150 155 160

Thr Asn Phe Pro Glu Leu Asp Glu Lys Gly Tyr Thr Thr Val Ile Ser

165 170 175

Ser Ile Pro Glu Trp Thr Asp Leu Gln Phe Thr Val Asp Leu Asn Gly

180 185 190

Thr Asn Trp Phe Asn Pro Gln Ser Val Leu Ile Asp Asp Val Ile Asn

195 200 205

Tyr Asn Gln Asn Leu Ser Met Lys Asp Gly Ile Val Ser Thr Asn Met

210 215 220

Asp Trp Leu Asn Gly Met Ile Asn Ile Lys Ser Glu Val Trp Ala His

225 230 235 240

Arg Lys Ile His Ser Leu Gly Ile Thr Arg Leu Glu Ile Ser Leu Asn

245 250 255

Leu Asp Ala Leu Pro Asp Glu Phe Thr Glu Leu Pro Val Thr Ile Tyr

260 265 270

Asp Ile Ile Asp Phe Asn Thr Ser His Arg Thr Thr Leu Tyr Glu Lys

275 280 285

Gly Gln Asp Glu Asp Asn Lys Ala Ile Tyr Met Ile Val Asn Pro Glu

290 295 300

Asn Val Pro Tyr Ser Asn Ala Val Val Tyr Ser Thr Cys Thr Ile Lys

305 310 315 320

Gly Thr Glu Asn Asn Phe Ser Pro Tyr Asn Phe Thr Ser Asp Asp Arg

325 330 335

Ile Ala Arg Asn Tyr Met Thr Asn Leu Thr Glu Glu Asn Pro Lys Val

340 345 350

Val Ile Tyr Lys Tyr Thr Ser Val Val Ser Ser Glu Tyr Asn Asn Asp

355 360 365

Glu Pro Asn Pro Asn Val Asn Leu Lys Phe Ala Ser Asn Ile Ala Asn

370 375 380

Thr Ala Lys Gly Asn Tyr Lys Ser Leu Leu Ser Asn His Lys Arg Ala

385 390 395 400

Trp Tyr Asp Leu Tyr Asn Asp Ala Phe Ile Glu Ile Pro Ser Asp Ser

405 410 415

Leu Leu Glu Met Thr Ala Arg Ser Ser Leu Phe His Leu Leu Ala Asn

420 425 430

Thr Arg Gln Tyr Asn Val Ser Thr Thr Arg Gly Leu Pro Val Gly Val

435 440 445

Gly Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Ala

450 455 460

Asp Val Trp Met Ala Pro Ala Leu Leu Pro Phe Phe Pro Asn Ile Ala

465 470 475 480

Met Asn Met Asn Asn Tyr Arg Asn Ala Thr His Gln Gln Ala Ile Glu

485 490 495

Asn Ala Lys Gln Tyr Asn Tyr Pro Gly Ala Val Tyr Pro Trp Thr Ser

500 505 510

Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Ile Asp Tyr Glu

515 520 525

Tyr His Ile Asn Val Asp Ile Ala Leu Ala Ser Phe Ser Ile Tyr Met

530 535 540

Asn Gly Ala Glu Gly Ala Asp Glu Asp Tyr Leu Arg Phe Thr Thr Trp

545 550 555 560

Pro Met Val Lys Asp Ala Ala Val Phe Phe Lys Ala Tyr Val Lys Tyr

565 570 575

Asn Glu Thr Leu Gly Glu Tyr Glu Thr Tyr Asn Leu Thr Asp Pro Asp

580 585 590

Glu Phe Ala Asn His Val Asn Asn Gly Ala Phe Thr Asn Ala Gly Ile

595 600 605

Lys Thr Leu Leu Lys Trp Ala Thr Asp Ile Gly Thr His Leu Gly Glu

610 615 620

Glu Val Asp Pro Lys Trp Met Glu Ile Ala Asp Asn Ile His Ile Pro

625 630 635 640

Arg Ser Asp Ser Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser

645 650 655

Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val Tyr Pro Leu Gly

660 665 670

Tyr Ile Asn Asp Glu Ser Ile Leu Asn Asn Ala Ile Lys Asp Leu Tyr

675 680 685

Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro

690 695 700

Val Phe Val Ala Ala Ala Ala Ser Leu Leu Asn His Gly Ser Ser Ser

705 710 715 720

Gln Ser Tyr Leu Tyr Lys Ser Val Leu Pro Tyr Leu Arg Ser Pro Phe

725 730 735

Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu

740 745 750

Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Phe Leu Gln

755 760 765

Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr Glu Val Thr

770 775 780

Pro Arg Thr Lys Lys Ile Ser Arg Leu Leu Lys Phe Asp Pro Val Lys

785 790 795 800

Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn Phe Lys Tyr Met

805 810 815

Gly Gln Val Leu Asp Ile Ile Ile Asp Asp Asn Asn Gly Thr Ile Ala

820 825 830

His Lys Gly Gly Asp Lys Pro Ile Arg Ile Lys Val Pro Asn Arg Asp

835 840 845

Ile Leu His Asp Arg Asn Ile Thr Ser Ala Leu Tyr Ser Lys Arg Asp

850 855 860

Asp Asp Leu Ser Ala Thr Asp Asp Tyr Tyr Gly Thr Tyr Phe Thr Leu

865 870 875 880

Tyr Pro Asn Glu Glu Leu Val Ile Pro Leu Tyr Asp Thr Lys Leu Asn

885 890 895

Ile Asp Gly Asn Ile Ala Glu Ser Lys Gln Ile Thr Asn Leu Thr Ala

900 905 910

Gly Val Pro Gly Asp Val Gly Phe Ser Ala Leu Asp Gly Asn Asn Tyr

915 920 925

Thr His Trp Gln Pro Phe Asp Lys Ser Asp Asn Ala Lys Leu Leu Ile

930 935 940

Asp Leu Gly Phe Asn Ser Thr His Val Ile Lys Lys Gly Ile Ile Leu

945 950 955 960

Trp Gly Gln Arg Pro Ala Lys Asn Ile Ser Leu Ser Val Leu Pro His

965 970 975

Ser Glu Arg Ile Glu Gln Leu Phe Ala Asn Ile Thr Asp Leu Leu Glu

980 985 990

Thr Ser Ser Ile Thr Lys Gly Gly Ser Leu Leu Asn Gln Met Leu Gly

995 1000 1005

Gln Thr Gln Ser Asn Val Thr Ala Glu Ile Asp Asp Asp Ile Leu

1010 1015 1020

Ala Leu Leu Asn Trp Lys Gly Asp Asp Leu Asp Gln Leu Ile Pro

1025 1030 1035

Tyr Leu Pro Asp Met His Leu Leu Gln Glu Lys Phe Ile Pro Ile

1040 1045 1050

Leu Lys Asp Tyr Pro Ile Lys Pro Asn Gln Arg Tyr Tyr Lys Glu

1055 1060 1065

Ile Ile Asp Asp Asp Ile Ile Lys Leu Leu Pro Ser Asn Thr Thr

1070 1075 1080

Glu Phe Thr Ile Asp Tyr Asn Ser Ile Pro Gly Gly Glu Lys Arg

1085 1090 1095

Ala Arg Tyr Val Val Leu Thr Val His Gly Thr Tyr Asp Asp Asp

1100 1105 1110

Asp Asp Leu Lys Gly Ala Thr Ile Lys Glu Ile Val Leu Gln Glu

1115 1120 1125

<210> 15

<211> 9382

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体光滑假丝酵母和酿酒酵母共同组成的序列

<400> 15

aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg 60

gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct 120

tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta 180

atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta 240

ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc 300

tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt 360

agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga 420

acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca 480

gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat 540

cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat 600

gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt 660

tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa 720

aaagaataaa aaaaaaatga tgaattgaat tgaaaagcgt ggtgcactct cagtacaatc 780

tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 840

tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 900

tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 960

atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagga cggatcgctt 1020

gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct 1080

gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa 1140

gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt 1200

actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa 1260

cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg 1320

aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg 1380

gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc 1440

tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat 1500

ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac 1560

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 1620

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 1680

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 1740

ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 1800

tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 1860

cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 1920

actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 1980

aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 2040

tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 2100

tttttttcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 2160

tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 2220

gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 2280

gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 2340

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 2400

gccagatggt aagccctccc gtatcgtagt tatctacacg acgggcagtc aggcaactat 2460

ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 2520

gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 2580

aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 2640

ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 2700

ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 2760

tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 2820

gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 2880

agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 2940

taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 3000

gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 3060

gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga 3120

caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 3180

gaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 3240

tttgtgatgc tcgtcagggg ggccgagcct atggaaaaac gccagcaacg cggccttttt 3300

acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 3360

ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 3420

gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 3480

tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 3540

agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg caccccaggc 3600

tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat aacaatttca 3660

cacaggaaac agctatgacc atgattacgc caagctcgga attaaccctc actaaaggga 3720

acaaaagctg ggtaccgggc cccccctcga gatctcccga gtttatcatt atcaatactg 3780

ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa 3840

aaaattggcc ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca 3900

cagaatatat aacatcatag gtgtctgggt gaacagttta ttcctggcat ccactaaata 3960

taatggagcc cgcttttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa 4020

tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca actacacaga 4080

acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcttg 4140

gagtaaatga tgacacaagg caattgacct acgcatgtat ctatctcatt ttcttacacc 4200

ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt 4260

tccctgaaat tattccccta tttgactaat aagtatataa agacggtagg tattgattgt 4320

aattctgtaa atctatttct taaacttctt aaattctact tttatagtta gtcttttttt 4380

tagttttaaa acactaagaa cttagtttcg aataaacaca cataaaaaca aatctagaat 4440

gggtttcaag gacaagattc tgttctggaa agacgaagtt caatatagaa cacttgcagt 4500

tgctgatcaa gtggccaaca gattctttca tagcttcgaa aatgtataca agggtgatga 4560

gtctgtcgaa gatgcggatt caagacctgt tggtttgacc aatgaaaccc tatcacattc 4620

atctgatttc tttgttttgc cagaagagag aatctctact agagtcaaga ttagacgtca 4680

gaatatccta aacacaacac tcattttggg gatgctgatc gcacttgtaa tatggactgc 4740

cattttgtct acaaactctt acttctcttc ctccttagct tctgcttcac cattattcaa 4800

caaagagggc agagtggtta gaccaatgcg tgaaagtaac ttaggcctac acgcagatcc 4860

tcaaactaga aaatccagta agacacttta cgatttgttg tcagactttg acaatgcatt 4920

ttacgatgat gaaaacatga tcttaggatc cctagccttc ggtgagaata cctactccag 4980

acagccatac gtcgcaaacg ggtacatagg tagccgtata ccaaacatcg gtttcggata 5040

cgcacttgat acacttaact tgtacgctga tgcaccaggc gcacttaaca acggttggcc 5100

tttgagaaac agaagattcg ctggaagttt cgtttctgat ttctactcac tgcaagccaa 5160

gttgaacagt accaattttc ctgagttaga tgaaaagggt tatacaactg tcatttcatc 5220

aatcccagaa tggacagatt tgcaattcac tgtagacctt aacggcacta actggttcaa 5280

tccacaatca gttctaatcg atgatgttat taattacaat cagaacttga gcatgaagga 5340

tggaattgtt agcacaaaca tggattggtt gaacggaatg ataaacatta agtctgaagt 5400

ctgggcacac agaaagatcc attccttggg tatcacacgt ctggaaatct cattgaacct 5460

tgatgcttta cctgacgagt tcactgaact accagttaca atttacgata tcatagattt 5520

caacacttct catagaacaa cactatatga gaaaggtcaa gatgaagata acaaagcgat 5580

ttacatgatt gtaaacccag aaaacgtacc atactcaaac gcagttgtgt actctacttg 5640

tacaatcaag ggcactgaaa acaacttttc tccttacaac ttcacatcag atgatagaat 5700

tgcgagaaac tacatgacaa acctaacaga agagaaccca aaagtcgtta tttacaagta 5760

cacatcagtg gtttcctcag aatataataa tgacgagcca aatcctaacg ttaacttgaa 5820

gttcgcctca aacatcgcta acaccgcaaa aggaaactat aagtctttat tgtccaatca 5880

caaaagagct tggtacgatt tgtacaatga tgcctttatc gaaattccat ctgatagttt 5940

attggaaatg accgcaagat ctagtctctt ccatttgctt gcgaacacac gtcaatacaa 6000

cgtttctaca actagaggat taccagtcgg cgttggtggt ctttcttcag actcttacgg 6060

ggggatggtc ttttgggacg cagacgtatg gatggctcca gctctcctac ctttctttcc 6120

aaacatagca atgaacatga ataactacag aaacgctact catcaacaag ccattgaaaa 6180

tgctaagcaa tacaattacc caggcgccgt ttacccttgg acatctggta gatatgcgaa 6240

ttgtacctct accggtcctt gcatcgacta cgaataccat attaatgtag atatcgcttt 6300

ggcaagtttc tctatttaca tgaacggtgc cgagggtgcc gacgaagatt accttagatt 6360

cacaacatgg ccaatggtca aggatgctgc agtgttcttc aaggcctacg tcaaatacaa 6420

tgaaacactt ggagagtacg aaacttataa tttaactgac ccagatgaat ttgctaatca 6480

tgtgaataat ggagctttca caaatgcagg aatcaaaact ttgttgaaat gggcaacaga 6540

cattggtact caccttgggg aggaagttga tcctaagtgg atggaaattg ctgataacat 6600

acatattcct agaagtgact ctaacattac tttggagtac tctggcatga atagctccgt 6660

tgagattaag caggctgacg ttacattgat ggtataccct ttaggttaca tcaacgatga 6720

atctatacta aacaacgcaa tcaaggattt gtattactac tctgaacgtc agtctgcctc 6780

tgggccagct atgacttacc cagtgttcgt cgcagctgcc gcttccctct tgaaccacgg 6840

ttcatcatca cagagttacc tttacaaatc tgttcttcca tatctgagat caccatttgc 6900

tcaatttagc gaacaatcag atgacaattt cttaactaac ggtttgacac aaccagcatt 6960

cccatttttg accgctaatg gtggtttctt gcaatctata ttatttggat taaccggatt 7020

gagatacagt tacgaagtta ctcctagaac caaaaagatt tcaagactac tcaagtttga 7080

tccagtcaag ttgccactgc tacctggcgg aattgcaatt agaaatttca aatacatggg 7140

acaagtactg gatatcataa tagacgataa caatggtaca atagctcata agggtgggga 7200

taagccaatt agaatcaagg ttccaaatag agatattctc cacgacagaa atatcacttc 7260

cgcactatac tcaaagagag atgacgattt atcagcgaca gatgactact acggtacata 7320

cttcacacta tacccaaatg aggaactggt gattcctctt tacgatacta agttaaacat 7380

cgacggtaac atagccgaat ctaaacaaat aactaatttg actgccggag ttccaggtga 7440

cgttggtttc tccgcattag atgggaacaa ttacacacac tggcagccat tcgataaatc 7500

agataatgct aagttgctaa ttgacttagg ttttaacagc actcatgtca ttaaaaaggg 7560

tattattttg tggggtcaaa gaccagcgaa aaacatttct ttgtcagttt tgccacattc 7620

agagagaatt gagcaactat ttgcgaacat cacagatctt ttagaaacat catctataac 7680

taagggcggc tctttgttaa accagatgtt aggccaaaca caatcaaacg tgaccgcaga 7740

aattgatgat gatatcctcg ctttactgaa ctggaaaggt gacgatctgg accaactcat 7800

cccttatttg cctgatatgc atctcctaca agagaaattc attcctattt tgaaggatta 7860

ccctattaag ccaaatcaga gatactataa ggaaatcatt gacgatgaca taatcaagtt 7920

attaccatca aacacaactg aatttacaat cgattacaat tctatccctg gcggggaaaa 7980

gcgtgcaaga tacgttgttc tgacagttca cggcacctat gatgacgatg atgatcttaa 8040

aggtgcaaca atcaaggaaa ttgttctgca ggaatgatta attaaacagg ccccttttcc 8100

tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc 8160

gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt 8220

atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac 8280

aaacgcgtgt acgcatgtaa cgggcagacg cggccgccac cgcggtggag ctccaattcg 8340

ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa 8400

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccccttcgc cagctggcgt 8460

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 8520

tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 8580

gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 8640

ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 8700

cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 8760

agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 8820

aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 8880

gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 8940

aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcctgatg cggtattttc 9000

tccttacgca tctgtgcggt atttcacacc gcagggtaat aactgatata attaaattga 9060

agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg 9120

ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac 9180

cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt 9240

agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa 9300

acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct 9360

ttgagcaata aagccgataa ca 9382

<210> 16

<211> 515

<212> PRT

<213> 扣囊复膜酵母菌(Saccharomycopsis fibuligera)

<400> 16

Met Ile Arg Leu Thr Val Phe Leu Thr Ala Val Phe Ala Ala Val Ala

1 5 10 15

Ser Cys Val Pro Val Glu Leu Asp Lys Arg Asn Thr Gly His Phe Gln

20 25 30

Ala Tyr Ser Gly Tyr Thr Val Ala Arg Ser Asn Phe Thr Gln Trp Ile

35 40 45

His Glu Gln Pro Ala Val Ser Trp Tyr Tyr Leu Leu Gln Asn Ile Asp

50 55 60

Tyr Pro Glu Gly Gln Phe Lys Ser Ala Lys Pro Gly Val Val Val Ala

65 70 75 80

Ser Pro Ser Thr Ser Glu Pro Asp Tyr Phe Tyr Gln Trp Thr Arg Asp

85 90 95

Thr Ala Ile Thr Phe Leu Ser Leu Ile Ala Glu Val Glu Asp His Ser

100 105 110

Phe Ser Asn Thr Thr Leu Ala Lys Val Val Glu Tyr Tyr Ile Ser Asn

115 120 125

Thr Tyr Thr Leu Gln Arg Val Ser Asn Pro Ser Gly Asn Phe Asp Ser

130 135 140

Pro Asn His Asp Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Asp Thr

145 150 155 160

Ala Tyr Thr Ala Ser Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Leu

165 170 175

Arg Ala Tyr Ala Ile Ser Arg Tyr Leu Asn Ala Val Ala Lys His Asn

180 185 190

Asn Gly Lys Leu Leu Leu Ala Gly Gln Asn Gly Ile Pro Tyr Ser Ser

195 200 205

Ala Ser Asp Ile Tyr Trp Lys Ile Ile Lys Pro Asp Leu Gln His Val

210 215 220

Ser Thr His Trp Ser Thr Ser Gly Phe Asp Leu Trp Glu Glu Asn Gln

225 230 235 240

Gly Thr His Phe Phe Thr Ala Leu Val Gln Leu Lys Ala Leu Ser Tyr

245 250 255

Gly Ile Pro Leu Ser Lys Thr Tyr Asn Asp Pro Gly Phe Thr Ser Trp

260 265 270

Leu Glu Lys Gln Lys Asp Ala Leu Asn Ser Tyr Ile Asn Ser Ser Gly

275 280 285

Phe Val Asn Ser Gly Lys Lys His Ile Val Glu Ser Pro Gln Leu Ser

290 295 300

Ser Arg Gly Gly Leu Asp Ser Ala Thr Tyr Ile Ala Ala Leu Ile Thr

305 310 315 320

His Asp Ile Gly Asp Asp Asp Thr Tyr Thr Pro Phe Asn Val Asp Asn

325 330 335

Ser Tyr Val Leu Asn Ser Leu Tyr Tyr Leu Leu Val Asp Asn Lys Asn

340 345 350

Arg Tyr Lys Ile Asn Gly Asn Tyr Lys Ala Gly Ala Ala Val Gly Arg

355 360 365

Tyr Pro Glu Asp Val Tyr Asn Gly Val Gly Thr Ser Glu Gly Asn Pro

370 375 380

Trp Gln Leu Ala Thr Ala Tyr Ala Gly Gln Thr Phe Tyr Thr Leu Ala

385 390 395 400

Tyr Asn Ser Leu Lys Asn Lys Lys Asn Leu Val Ile Glu Lys Leu Asn

405 410 415

Tyr Asp Leu Tyr Asn Ser Phe Ile Ala Asp Leu Ser Lys Ile Asp Ser

420 425 430

Ser Tyr Ala Ser Lys Asp Ser Leu Thr Leu Thr Tyr Gly Ser Asp Asn

435 440 445

Tyr Lys Asn Val Ile Lys Ser Leu Leu Gln Phe Gly Asp Ser Phe Leu

450 455 460

Lys Val Leu Leu Asp His Ile Asp Asp Asn Gly Gln Leu Thr Glu Glu

465 470 475 480

Ile Asn Arg Tyr Thr Gly Phe Gln Ala Gly Ala Val Ser Leu Thr Trp

485 490 495

Ser Ser Gly Ser Leu Leu Ser Ala Asn Arg Ala Arg Asn Lys Leu Ile

500 505 510

Glu Leu Leu

515

<210> 17

<211> 579

<212> PRT

<213> 米根霉(Rhizopus oryzae)

<400> 17

Ala Ser Ile Pro Ser Ser Ala Ser Val Gln Leu Asp Ser Tyr Asn Tyr

1 5 10 15

Asp Gly Ser Thr Phe Ser Gly Lys Ile Tyr Val Lys Asn Ile Ala Tyr

20 25 30

Ser Lys Lys Val Thr Val Val Tyr Ala Asp Gly Ser Asp Asn Trp Asn

35 40 45

Asn Asn Gly Asn Thr Ile Ala Ala Ser Phe Ser Gly Pro Ile Ser Gly

50 55 60

Ser Asn Tyr Glu Tyr Trp Thr Phe Ser Ala Ser Val Lys Gly Ile Lys

65 70 75 80

Glu Phe Tyr Ile Lys Tyr Glu Val Ser Gly Lys Thr Tyr Tyr Asp Asn

85 90 95

Asn Asn Ser Ala Asn Tyr Gln Val Ser Thr Ser Lys Pro Thr Thr Thr

100 105 110

Thr Ala Ala Thr Thr Thr Thr Thr Ala Pro Ser Thr Ser Thr Thr Thr

115 120 125

Arg Pro Ser Ser Ser Glu Pro Ala Thr Phe Pro Thr Gly Asn Ser Thr

130 135 140

Ile Ser Ser Trp Ile Lys Lys Gln Glu Asp Ile Ser Arg Phe Ala Met

145 150 155 160

Leu Arg Asn Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala

165 170 175

Ser Leu Ser Thr Ala Gly Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp

180 185 190

Ala Ala Leu Thr Ser Asn Val Ile Val Tyr Glu Tyr Asn Thr Thr Leu

195 200 205

Ser Gly Asn Lys Thr Ile Leu Asn Val Leu Lys Asp Tyr Val Thr Phe

210 215 220

Ser Val Lys Thr Gln Ser Thr Ser Thr Val Cys Asn Cys Leu Gly Glu

225 230 235 240

Pro Lys Phe Asn Pro Asp Gly Ser Gly Tyr Thr Gly Ala Trp Gly Arg

245 250 255

Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe Val Leu Phe

260 265 270

Ala Asp Ser Tyr Leu Thr Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly

275 280 285

Thr Leu Lys Pro Ala Ile Phe Lys Asp Leu Asp Tyr Val Val Asn Val

290 295 300

Trp Ser Asn Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His

305 310 315 320

Phe Tyr Thr Leu Met Val Met Arg Lys Gly Leu Leu Leu Gly Ala Asp

325 330 335

Phe Ala Lys Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Ser Ser

340 345 350

Thr Ala Ser Thr Ile Ala Asn Lys Ile Ser Ser Phe Trp Val Ser Ser

355 360 365

Asn Asn Trp Val Gln Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys

370 375 380

Lys Gly Leu Asp Val Ser Thr Leu Leu Ala Ala Asn Leu Gly Ser Val

385 390 395 400

Asp Asp Gly Phe Phe Thr Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala

405 410 415

Val Ala Val Glu Asp Ser Phe Ala Ser Leu Tyr Pro Ile Asn Lys Asn

420 425 430

Leu Pro Ser Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr

435 440 445

Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Val

450 455 460

Thr Gly Tyr Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Ile Ser

465 470 475 480

Asn Gly Gly Val Thr Val Ser Ser Ile Ser Leu Pro Phe Phe Lys Lys

485 490 495

Phe Asp Ser Ser Ala Thr Ser Gly Lys Lys Tyr Thr Val Gly Thr Ser

500 505 510

Asp Phe Asn Asn Leu Ala Gln Asn Ile Ala Leu Ala Ala Asp Arg Phe

515 520 525

Leu Ser Thr Val Gln Leu His Ala Pro Asn Asn Gly Ser Leu Ala Glu

530 535 540

Glu Phe Asp Arg Thr Thr Gly Phe Ser Thr Gly Ala Arg Asp Leu Thr

545 550 555 560

Trp Ser His Ala Ser Leu Ile Thr Ala Ser Tyr Ala Lys Ala Gly Ala

565 570 575

Pro Ala Ala

<210> 18

<211> 621

<212> PRT

<213> 白宇佐美曲霉(Aspergillus shirousami)

<400> 18

Ser Val Ile Ser Lys Arg Ala Thr Leu Asp Ser Trp Leu Ser Asn Glu

1 5 10 15

Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala Asp Gly

20 25 30

Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser Pro Ser

35 40 45

Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser Gly Ile

50 55 60

Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr Asp Leu

65 70 75 80

Leu Ser Thr Ile Glu His Tyr Ile Ser Ser Gln Ala Ile Ile Gln Gly

85 90 95

Val Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Gly Leu Gly Glu Pro

100 105 110

Lys Phe Asn Val Asp Glu Thr Ala Tyr Ala Gly Ser Trp Gly Arg Pro

115 120 125

Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Gly Phe Gly

130 135 140

Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Ala Ala Thr Glu Ile Val

145 150 155 160

Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln Tyr Trp Asn

165 170 175

Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser Ser Phe Phe

180 185 190

Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser Ala Phe Ala

195 200 205

Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln Ala Pro Gln

210 215 220

Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Tyr Ile Leu Ala

225 230 235 240

Asn Phe Asp Ser Ser Arg Ser Gly Lys Asp Thr Asn Thr Leu Leu Gly

245 250 255

Ser Ile His Thr Phe Asp Pro Glu Ala Gly Cys Asp Asp Ser Thr Phe

260 265 270

Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu Val Val Asp

275 280 285

Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser Asp Ser Glu

290 295 300

Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Ser Tyr Tyr Asn Gly Asn

305 310 315 320

Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu Tyr Asp Ala

325 330 335

Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Ile Thr Asp Val Ser

340 345 350

Leu Asp Phe Phe Lys Ala Leu Tyr Ser Gly Ala Ala Thr Gly Thr Tyr

355 360 365

Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Ser Ala Val Lys Thr

370 375 380

Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala Ala Ser Asn

385 390 395 400

Gly Ser Leu Ser Glu Gln Phe Asp Lys Ser Asp Gly Asp Glu Leu Ser

405 410 415

Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr Ala Asn Asn

420 425 430

Arg Arg Asn Ser Val Val Pro Pro Ser Trp Gly Glu Thr Ser Ala Ser

435 440 445

Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ser Gly Thr Tyr Ser

450 455 460

Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr Gly Gly Thr

465 470 475 480

Thr Thr Thr Ala Thr Thr Thr Gly Ser Gly Gly Val Thr Ser Thr Ser

485 490 495

Lys Thr Thr Thr Thr Ala Ser Lys Thr Ser Thr Thr Thr Ser Ser Thr

500 505 510

Ser Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu Thr Ala

515 520 525

Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln

530 535 540

Leu Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys

545 550 555 560

Tyr Thr Ser Ser Asn Pro Pro Trp Tyr Val Thr Val Thr Leu Pro Ala

565 570 575

Gly Glu Ser Phe Glu Tyr Lys Phe Ile Arg Val Glu Ser Asp Asp Ser

580 585 590

Val Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala

595 600 605

Cys Gly Glu Ser Thr Ala Thr Val Thr Asp Thr Trp Arg

610 615 620

<210> 19

<211> 616

<212> PRT

<213> 土曲霉(Aspergillus terreus)

<400> 19

Ala Pro Gln Leu Ala Pro Arg Ala Thr Thr Ser Leu Asp Ala Trp Leu

1 5 10 15

Ala Ser Glu Thr Thr Val Ala Leu Asp Gly Ile Leu Asp Asn Val Gly

20 25 30

Ser Ser Gly Ala Tyr Ala Lys Ser Ala Lys Ser Gly Ile Val Ile Ala

35 40 45

Ser Pro Ser Thr Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp

50 55 60

Ala Ala Leu Thr Val Lys Ala Leu Ile Asp Leu Phe Arg Asn Gly Glu

65 70 75 80

Thr Ser Leu Gln Thr Val Ile Met Glu Tyr Ile Ser Ser Gln Ala Tyr

85 90 95

Leu Gln Thr Val Ser Asn Pro Ser Gly Ser Leu Ser Thr Gly Gly Leu

100 105 110

Ala Glu Pro Lys Tyr Tyr Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp

115 120 125

Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile

130 135 140

Asp Phe Gly Asn Trp Leu Ile Asp Asn Gly Tyr Ser Thr Tyr Ala Ser

145 150 155 160

Ser Ile Val Trp Pro Ile Val Arg Asn Asp Leu Ser Tyr Val Ala Gln

165 170 175

Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser

180 185 190

Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser

195 200 205

Thr Phe Ala Ser Lys Val Gly Ala Ser Cys Ser Trp Cys Asp Ser Gln

210 215 220

Ala Pro Gln Val Leu Cys Phe Leu Gln Arg Phe Trp Thr Gly Ser Tyr

225 230 235 240

Ile Met Ala Asn Phe Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr

245 250 255

Val Leu Gly Ser Ile His Thr Phe Asp Pro Asn Ala Gly Cys Asp Asp

260 265 270

Thr Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Val

275 280 285

Tyr Thr Asp Ser Phe Arg Ser Ile Tyr Ser Ile Asn Ser Gly Ile Ser

290 295 300

Ser Gly Lys Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Ser Tyr Tyr

305 310 315 320

Asn Gly Asn Pro Trp Phe Leu Thr Thr Leu Ala Ala Ala Glu Gln Leu

325 330 335

Tyr Asp Ala Ile Tyr Gln Trp Gln Lys Ile Gly Ser Ile Thr Ile Thr

340 345 350

Asp Val Ser Leu Ala Phe Phe Lys Asp Leu Tyr Ser Ser Ala Ala Val

355 360 365

Gly Thr Tyr Ala Ser Ser Ser Ser Ala Phe Thr Ser Ile Val Ser Ala

370 375 380

Val Lys Thr Tyr Ala Asp Gly Tyr Met Ser Ile Val Gln Thr His Ala

385 390 395 400

Met Thr Asn Gly Ser Leu Ser Glu Gln Phe Gly Lys Ser Asp Gly Phe

405 410 415

Ser Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr

420 425 430

Ala Asn Leu Arg Arg Asn Ser Val Val Pro Pro Ser Trp Gly Glu Thr

435 440 445

Thr Ala Thr Ser Val Pro Ser Val Cys Ser Ala Thr Ser Ala Thr Gly

450 455 460

Thr Tyr Ser Thr Ala Thr Asn Thr Ala Trp Pro Ser Thr Leu Thr Ser

465 470 475 480

Gly Thr Gly Ala Thr Thr Thr Thr Ser Lys Ala Thr Ser Ser Ser Thr

485 490 495

Thr Thr Thr Ser Ser Ala Ser Ser Thr Thr Val Glu Cys Val Val Pro

500 505 510

Thr Ala Val Ala Val Thr Phe Asp Glu Val Ala Thr Thr Thr Tyr Gly

515 520 525

Glu Asn Val Tyr Val Val Gly Ser Ile Ser Gln Leu Gly Ser Trp Asp

530 535 540

Thr Ser Lys Ala Val Ala Leu Ser Ala Ser Lys Tyr Thr Ser Ser Asn

545 550 555 560

Asn Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly Thr Thr Phe Gln

565 570 575

Tyr Lys Phe Ile Arg Val Ser Ser Ser Gly Ser Val Thr Trp Glu Ser

580 585 590

Asp Pro Asn Arg Ser Tyr Thr Val Pro Ser Ala Cys Gly Thr Ser Thr

595 600 605

Ala Val Val Asn Thr Thr Trp Arg

610 615

<210> 20

<211> 617

<212> PRT

<213> 米卡塔酵母(Saccharomyces mikatae)

<400> 20

Met Lys Asn Phe Ile Ser Leu Val Asn Lys Lys Lys Gly Thr Leu Asp

1 5 10 15

Asp Arg Asn Ser Ser Val Pro Glu Ser Ser Ser Gly Ile Ile His Gln

20 25 30

Arg Gly Ala Leu Asn Thr Glu Asp Phe Glu Glu Gly Lys Lys Asp Gly

35 40 45

Ala Phe Glu Leu Gly His Leu Glu Phe Thr Thr Asn Ser Ala Gln Leu

50 55 60

Gly Asp Ser Asp Asp Asp Asn Asp Asn Ala Ile Lys Ile Ala Asn Ala

65 70 75 80

Ala Thr Asp Glu Ala Asn Glu Ala Asn Ser Glu Glu Lys Ser Met Thr

85 90 95

Leu Arg Gln Ala Leu Arg Lys Tyr Pro Lys Ala Ala Leu Trp Ser Ile

100 105 110

Leu Val Ser Thr Thr Leu Val Met Glu Gly Tyr Asp Thr Ala Leu Leu

115 120 125

Ser Ala Leu Tyr Ala Leu Pro Val Phe Gln Arg Lys Phe Gly Thr Met

130 135 140

Asn Ala Glu Gly Ser Tyr Glu Ile Thr Ser Gln Trp Gln Ile Gly Leu

145 150 155 160

Asn Met Cys Val Leu Cys Gly Glu Met Ile Gly Leu Gln Met Thr Thr

165 170 175

Tyr Met Val Glu Phe Met Gly Asn Arg Tyr Thr Met Ile Thr Ala Leu

180 185 190

Gly Leu Leu Thr Ala Tyr Ile Phe Ile Leu Tyr Tyr Cys Lys Ser Leu

195 200 205

Ala Met Ile Ala Val Gly Gln Ile Leu Ser Ala Met Pro Trp Gly Cys

210 215 220

Phe Gln Ser Leu Ala Val Thr Tyr Ala Ser Glu Val Cys Pro Leu Ala

225 230 235 240

Leu Arg Tyr Tyr Met Thr Ser Tyr Ser Asn Ile Cys Trp Leu Phe Gly

245 250 255

Gln Ile Phe Ala Ser Gly Ile Met Lys Asn Ser Gln Glu Asn Leu Gly

260 265 270

Asp Ser Asp Leu Gly Tyr Lys Leu Pro Phe Ala Leu Gln Trp Ile Trp

275 280 285

Pro Ala Pro Leu Ile Ile Gly Ile Phe Phe Ala Pro Glu Ser Pro Trp

290 295 300

Trp Leu Val Arg Lys Asn Lys Ile Ala Glu Ala Lys Lys Ser Leu Asn

305 310 315 320

Arg Ile Leu Ser Gly Thr Ala Ala Glu Arg Glu Ile Gln Val Asp Ile

325 330 335

Thr Leu Lys Gln Ile Glu Met Thr Ile Glu Lys Glu Arg Leu Leu Ala

340 345 350

Ser Lys Ser Gly Ser Phe Phe Asn Cys Phe Lys Gly Val Asp Gly Arg

355 360 365

Arg Thr Arg Leu Ala Cys Leu Thr Trp Val Ala Gln Asn Ser Ser Gly

370 375 380

Ala Val Leu Leu Gly Tyr Ser Thr Tyr Phe Phe Glu Arg Ala Gly Met

385 390 395 400

Ala Thr Asp Lys Ala Phe Thr Phe Ser Leu Ile Gln Tyr Cys Leu Gly

405 410 415

Leu Ala Gly Thr Leu Cys Ser Trp Val Ile Ser Gly Arg Val Gly Arg

420 425 430

Trp Ser Ile Leu Ala Tyr Gly Leu Ala Phe Gln Met Val Cys Leu Phe

435 440 445

Ile Ile Gly Gly Met Gly Phe Ala Ser Gly Ser Asn Ala Ser Asn Gly

450 455 460

Ala Gly Gly Leu Leu Leu Ala Leu Ser Phe Phe Tyr Asn Ala Gly Ile

465 470 475 480

Gly Ala Val Val Tyr Cys Ile Val Ala Glu Ile Pro Ser Ala Glu Leu

485 490 495

Arg Thr Lys Thr Ile Val Met Ala Arg Ile Cys Tyr Asn Leu Met Ala

500 505 510

Val Ile Asn Ala Ile Leu Thr Pro Tyr Met Leu Asn Val Ser Asp Trp

515 520 525

Asn Trp Gly Ala Lys Thr Gly Leu Tyr Trp Gly Gly Phe Thr Ala Val

530 535 540

Thr Leu Ala Trp Val Ile Ile Asp Leu Pro Glu Thr Thr Gly Arg Thr

545 550 555 560

Phe Ser Glu Ile Asn Glu Leu Phe Asn Gln Gly Val Pro Ala Arg Lys

565 570 575

Phe Ala Ser Thr Val Val Asp Pro Phe Gly Lys Gly Gln Arg Gln Asn

580 585 590

Asp Ser Gln Val Asp Asn Val Ile Asp Gln Ser Ser Ser Ala Met Gln

595 600 605

Gln Glu Leu Asn Glu Ala Asn Glu Phe

610 615

<210> 21

<211> 1928

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 21

caagctatac caagcataca atcaactatc tcatatacaa tgaaaaacat catttcatta 60

gtttctaaga agaaggctgc atccaaaaat gaagataaaa acatctctga atcttcaaga 120

gatatcgtta atcaacaaga ggttttcaat accgaagatt tcgaggaagg caaaaaggac 180

tctgcattcg aattagatca cttggagttt actaccaact ccgcacaatt aggcgactct 240

gatgaggaca acgaaaacgt cattaacgaa atgaacgcaa cagacgatgc aaacgaagca 300

aactctgaag agaaatccat gaccttgaag caagcattgt tgaagtatcc taaagccgca 360

ttatggtcta ttttagtctc cactactttg gtcatggaag gttacgacac tgctttgtta 420

tccgcattat acgctttacc agtttttcaa cgtaagtttg gtacattgaa tggtgaaggt 480

tcctatgaga ttacatccca atggcagatt ggtcttaaca tgtgcgtctt gtgtggtgaa 540

atgattggtt tacaaatcac cacctatatg gttgagttta tgggtaacag atacactatg 600

atcaccgctt taggtctttt gactgcttac attttcattt tgtactattg taagtcctta 660

gccatgattg ctgttggtca aattttgtcc gccatccctt ggggttgttt tcaatctttg 720

gctgttacct atgcttctga agtctgccca ttagcactta gatactatat gacttcctat 780

tctaacattt gctggttgtt cggccaaatc ttcgcatctg gtatcatgaa aaactcacaa 840

gagaacttag gcaattccga tcttggttac aaacttccat ttgctttaca gtggatttgg 900

cctgccccac ttatgattgg tattttcttt gctccagaat ctccttggtg gcttgttaga 960

aaagatagag ttgcagaagc tagaaagtca ttatccagaa ttttgtctgg taagggcgct 1020

gaaaaggata ttcaagttga tcttacctta aagcagattg aattaactat tgaaaaggaa 1080

agattattag cttctaagtc tggttcattc tttaactgtt tcaagggtgt taatggtcgt 1140

agaaccagat tagcatgttt aacctgggtt gcccaaaact cctccggtgc agttttgtta 1200

ggttattcta cttacttttt cgaaagagct ggtatggcaa cagacaaggc tttcacattc 1260

tcattaatcc agtactgttt gggtcttgca ggcaccttat gttcatgggt tatttcaggt 1320

agagtcggta gatggactat cttgacttat ggtcttgctt ttcaaatggt ctgtttgttc 1380

attatcggtg gtatgggttt tggttctggt tcctctgctt ccaatggtgc tggtggctta 1440

ttgttggcat tatccttttt ctacaatgca ggcatcggtg ctgttgtcta ttgtattgtc 1500

gccgaaattc cttcagcaga attgagaacc aagactattg ttttggctag aatctgttac 1560

aatttgatgg ccgttatcaa tgctattttg accccataca tgttgaacgt ttctgactgg 1620

aactggggtg ctaagacagg cttgtattgg ggtggtttca ctgcagttac tttggcttgg 1680

gttattatcg acttaccaga aaccactggt agaacattct ctgaaatcaa tgaattattc 1740

aaccaaggtg ttccagctcg taagttcgca tctactgtcg tcgatccatt cggtaagggt 1800

aagacccaac atgattcctt ggccgatgaa tccatctctc aatcttcatc cattaagcaa 1860

agagaattga atgcagcaga taagtgttaa ttaattaaac aggccccttt tcctttgtcg 1920

atatcatg 1928

<210> 22

<211> 523

<212> PRT

<213> 东方伊萨酵母(Issatchenkia orientalis)

<400> 22

Met Ser Asp Leu Glu Ser Gln His Thr Arg Glu Glu Leu Asp Lys Ser

1 5 10 15

Glu Asp Glu Glu Ile Gln Leu Ile Asn Glu Ser Arg Pro Pro Lys Leu

20 25 30

Asp Lys Glu Thr Ile Lys Arg Tyr Leu Lys Thr Arg Phe Thr Glu Leu

35 40 45

Leu Pro Thr Lys Gln Gln Met Lys Gln Asn Lys His Leu Leu Asn Pro

50 55 60

Leu Pro Gly Leu Arg Met Met Gly Arg Arg Gln Trp Leu Met Phe Leu

65 70 75 80

Ser Ala Phe Cys Ala Trp Thr Trp Asp Ala Tyr Asp Phe Phe Thr Val

85 90 95

Ser Leu Asn Thr Val Gln Leu Ala Lys Asp Phe Asp Lys Asn Val Lys

100 105 110

Asp Ile Thr Trp Gly Ile Thr Leu Val Leu Met Leu Arg Ser Val Gly

115 120 125

Gly Ile Thr Phe Gly Tyr Leu Gly Asp Arg Tyr Gly Arg Lys Trp Pro

130 135 140

Leu Val Ala Asn Leu Leu Ile Val Cys Ile Leu Glu Ile Gly Ser Gly

145 150 155 160

Phe Ile Lys Thr Tyr Arg Gln Phe Leu Gly Val Arg Ala Leu Phe Gly

165 170 175

Ile Cys Leu Gly Gly Val Tyr Gly Asn Ala Thr Ala Thr Ala Leu Asp

180 185 190

Asp Cys Pro Thr Glu Ala Arg Gly Phe Ile Gly Gly Leu Leu Gln Gln

195 200 205

Gly Tyr Ala Phe Gly Tyr Leu Leu Ala Val Val Phe Lys Arg Ala Ile

210 215 220

Ala Asp Asn Ser Ser His Lys Trp Arg Ala Thr Phe Trp Phe Gly Ala

225 230 235 240

Gly Ile Ser Phe Leu Phe Ala Leu Phe Arg Ala Ser Leu Pro Glu Thr

245 250 255

Lys Ala Phe Leu Arg Lys Lys Glu Ile Glu Arg Tyr Asn Lys Glu His

260 265 270

Gly Ile Tyr Gln Pro Thr Phe Gln Met Lys Ile Val Glu Ser Leu Lys

275 280 285

Asn Tyr Trp Leu Met Met Ile Tyr Leu Val Leu Phe Met Ser Gly Phe

290 295 300

Ser Phe Met Ser His Ser Ser Gln Asp Leu Tyr Pro Thr Leu Leu Thr

305 310 315 320

Val Arg Tyr Ser Phe Ser Glu Asn Lys Ala Thr Val Thr Asn Cys Val

325 330 335

Ala Asn Ile Gly Ala Phe Ile Gly Gly Val Phe Thr Gly His Ile Ser

340 345 350

Asn Phe Leu Gly Arg Arg Leu Thr Ile Met Leu Ile Cys Ile Phe Gly

355 360 365

Gly Ala Leu Ile Tyr Pro Trp Ala Phe Ile Asp Gly Thr Gly Ile Asn

370 375 380

Ala Gly Val Phe Phe Leu Gln Trp Cys Val Gln Gly Gly Leu Gly Val

385 390 395 400

Val Pro Ser His Leu Ser Glu Leu Ala Pro Pro Asp Leu Arg Ala Phe

405 410 415

Val Val Gly Ile Ser Tyr Gln Leu Gly Asn Leu Ala Ala Ser Ala Ser

420 425 430

Ser Thr Ile Glu Thr Thr Ile Gly Glu Gln Phe Pro Met Thr Ser Pro

435 440 445

Ser Gly Glu Pro Ile Tyr Asp Tyr Ala Lys Val Met Ala Ile Phe Val

450 455 460

Gly Cys Val Phe Ala Phe Val Leu Ile Val Thr Leu Phe Gly Pro Glu

465 470 475 480

Arg Arg Asn Ala Ser Phe Glu Asn Ala Val Thr Thr Asn Glu Glu Phe

485 490 495

Gly Asn Ser Ala Gly Ala Val Asp Lys Ile Glu His Val Glu Glu Cys

500 505 510

Ile Val Pro Lys His Ala Arg Asn Gln Gln Cys

515 520

<210> 23

<211> 616

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 23

Met Ser Ser Ser Ile Thr Asp Glu Lys Ile Ser Gly Glu Gln Gln Gln

1 5 10 15

Pro Ala Gly Arg Lys Leu Tyr Tyr Asn Thr Ser Thr Phe Ala Glu Pro

20 25 30

Pro Leu Val Asp Gly Glu Gly Asn Pro Ile Asn Tyr Glu Pro Glu Val

35 40 45

Tyr Asn Pro Asp His Glu Lys Leu Tyr His Asn Pro Ser Leu Pro Ala

50 55 60

Gln Ser Ile Gln Asp Thr Arg Asp Asp Glu Leu Leu Glu Arg Val Tyr

65 70 75 80

Ser Gln Asp Gln Gly Val Glu Tyr Glu Glu Asp Glu Glu Asp Lys Pro

85 90 95

Asn Leu Ser Ala Ala Ser Ile Lys Ser Tyr Ala Leu Thr Arg Phe Thr

100 105 110

Ser Leu Leu His Ile His Glu Phe Ser Trp Glu Asn Val Asn Pro Ile

115 120 125

Pro Glu Leu Arg Lys Met Thr Trp Gln Asn Trp Asn Tyr Phe Phe Met

130 135 140

Gly Tyr Phe Ala Trp Leu Ser Ala Ala Trp Ala Phe Phe Cys Val Ser

145 150 155 160

Val Ser Val Ala Pro Leu Ala Glu Leu Tyr Asp Arg Pro Thr Lys Asp

165 170 175

Ile Thr Trp Gly Leu Gly Leu Val Leu Phe Val Arg Ser Ala Gly Ala

180 185 190

Val Ile Phe Gly Leu Trp Thr Asp Lys Ser Ser Arg Lys Trp Pro Tyr

195 200 205

Ile Thr Cys Leu Phe Leu Phe Val Ile Ala Gln Leu Cys Thr Pro Trp

210 215 220

Cys Asp Thr Tyr Glu Lys Phe Leu Gly Val Arg Trp Ile Thr Gly Ile

225 230 235 240

Ala Met Gly Gly Ile Tyr Gly Cys Ala Ser Ala Thr Ala Ile Glu Asp

245 250 255

Ala Pro Val Lys Ala Arg Ser Phe Leu Ser Gly Leu Phe Phe Ser Ala

260 265 270

Tyr Ala Met Gly Phe Ile Phe Ala Ile Ile Phe Tyr Arg Ala Phe Gly

275 280 285

Tyr Phe Arg Asp Asp Gly Trp Lys Ile Leu Phe Trp Phe Ser Ile Phe

290 295 300

Leu Pro Ile Leu Leu Ile Phe Trp Arg Leu Leu Trp Pro Glu Thr Lys

305 310 315 320

Tyr Phe Thr Lys Val Leu Lys Ala Arg Lys Leu Ile Leu Ser Asp Ala

325 330 335

Val Lys Ala Asn Gly Gly Glu Pro Leu Pro Lys Ala Asn Phe Lys Gln

340 345 350

Lys Met Val Ser Met Lys Arg Thr Val Gln Lys Tyr Trp Leu Leu Phe

355 360 365

Ala Tyr Leu Val Val Leu Leu Val Gly Pro Asn Tyr Leu Thr His Ala

370 375 380

Ser Gln Asp Leu Leu Pro Thr Met Leu Arg Ala Gln Leu Gly Leu Ser

385 390 395 400

Lys Asp Ala Val Thr Val Ile Val Val Val Thr Asn Ile Gly Ala Ile

405 410 415

Cys Gly Gly Met Ile Phe Gly Gln Phe Met Glu Val Thr Gly Arg Arg

420 425 430

Leu Gly Leu Leu Ile Ala Cys Thr Met Gly Gly Cys Phe Thr Tyr Pro

435 440 445

Ala Phe Met Leu Arg Ser Glu Lys Ala Ile Leu Gly Ala Gly Phe Met

450 455 460

Leu Tyr Phe Cys Val Phe Gly Val Trp Gly Ile Leu Pro Ile His Leu

465 470 475 480

Ala Glu Leu Ala Pro Ala Asp Ala Arg Ala Leu Val Ala Gly Leu Ser

485 490 495

Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ala Ser Thr Ile Glu Thr

500 505 510

Gln Leu Ala Asp Arg Tyr Pro Leu Glu Arg Asp Ala Ser Gly Ala Val

515 520 525

Ile Lys Glu Asp Tyr Ala Lys Val Met Ala Ile Leu Thr Gly Ser Val

530 535 540

Phe Ile Phe Thr Phe Ala Cys Val Phe Val Gly His Glu Lys Phe His

545 550 555 560

Arg Asp Leu Ser Ser Pro Val Met Lys Lys Tyr Ile Asn Gln Val Glu

565 570 575

Glu Tyr Glu Ala Asp Gly Leu Ser Ile Ser Asp Ile Val Glu Gln Lys

580 585 590

Thr Glu Cys Ala Ser Val Lys Met Ile Asp Ser Asn Val Ser Lys Thr

595 600 605

Tyr Glu Glu His Ile Glu Thr Val

610 615

<210> 24

<211> 600

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 24

Met Asn Asn Asn Asn Ile Thr Pro Glu Ser Asp Ser Met Lys Ser Asn

1 5 10 15

Asp Asn Asp Gln Thr Asn Asp Tyr Met Pro Asp Val Ala Asp Phe Asp

20 25 30

His Thr Gln Thr Asn Thr Asn Glu Ile Ala Arg Ala Ile Ser His Pro

35 40 45

Gly Ser Val Leu Ser Arg Val Ala Ser Tyr Val Ser Arg Lys Asp Arg

50 55 60

Tyr Val Asp Glu Asn Gly Asn Glu Val Trp Gln Asp Asp Glu Val Ser

65 70 75 80

Ile Leu Met Glu Glu Asp Glu Thr Pro Asp Phe Thr Trp Lys Asn Ile

85 90 95

Arg His Tyr Ala Ile Thr Arg Phe Thr Thr Leu Thr Glu Leu His Arg

100 105 110

Val Ser Met Glu Asn Ile Asn Pro Ile Pro Glu Leu Arg Lys Met Thr

115 120 125

Leu His Asn Trp Asn Tyr Phe Phe Met Gly Tyr Ala Ala Trp Leu Cys

130 135 140

Ala Ala Trp Ala Phe Phe Ala Val Ser Val Ser Thr Ala Pro Leu Ala

145 150 155 160

Thr Leu Tyr Gly Lys Glu Thr Lys Asp Ile Ser Trp Gly Leu Ser Leu

165 170 175

Val Leu Phe Val Arg Ser Ala Gly Ala Ile Ile Phe Gly Ile Trp Thr

180 185 190

Asp Asn Tyr Ser Arg Lys Trp Pro Tyr Ile Thr Cys Leu Gly Leu Phe

195 200 205

Leu Ile Cys Gln Leu Cys Thr Pro Trp Ala Lys Thr Tyr Thr Gln Phe

210 215 220

Leu Gly Val Arg Trp Ile Ser Gly Ile Ala Met Gly Gly Ile Tyr Ala

225 230 235 240

Cys Ala Ser Ala Thr Ala Ile Glu Asp Ala Pro Val Lys Ala Arg Ser

245 250 255

Phe Leu Ser Gly Leu Phe Phe Thr Ala Tyr Ala Met Gly Phe Ile Phe

260 265 270

Ala Ile Ile Phe Tyr Arg Ala Phe Leu Asn Val Asn Gly Glu Asn Tyr

275 280 285

Trp Lys Val Gln Phe Trp Phe Ser Ile Trp Leu Pro Ala Val Leu Ile

290 295 300

Leu Trp Arg Leu Val Trp Pro Glu Thr Lys Tyr Phe Thr Lys Val Leu

305 310 315 320

Lys Ala Arg Gln Leu Met Arg Asp Asp Ala Ile Ala Lys Asn Gly Gly

325 330 335

Gln Pro Leu Pro Lys Leu Ser Phe Lys Gln Lys Phe Ala Asn Val Lys

340 345 350

Lys Thr Val Ser Lys Tyr Trp Leu Leu Phe Gly Tyr Leu Ile Leu Leu

355 360 365

Leu Val Gly Pro Asn Tyr Leu Thr His Ala Ser Gln Asp Leu Phe Pro

370 375 380

Thr Met Leu Arg Ala Gln Leu Arg Phe Ser Glu Asp Ala Val Thr Val

385 390 395 400

Ala Ile Val Val Val Cys Leu Gly Ser Ile Ala Gly Gly Met Phe Phe

405 410 415

Gly Gln Leu Met Glu Ile Thr Gly Arg Arg Val Gly Leu Leu Leu Ala

420 425 430

Leu Ile Met Ala Gly Cys Phe Thr Tyr Pro Ala Phe Met Leu Lys Thr

435 440 445

Ser Ser Ala Val Leu Gly Ala Gly Phe Met Leu Trp Phe Ser Ile Leu

450 455 460

Gly Val Trp Gly Val Leu Pro Ile His Leu Ser Glu Leu Ser Pro Pro

465 470 475 480

Glu Ala Arg Ala Leu Val Ser Gly Leu Ala Tyr Gln Leu Gly Asn Leu

485 490 495

Ala Ser Ala Ala Ser Val Val Ile Glu Asn Asp Leu Ala Asp Leu Tyr

500 505 510

Pro Ile Glu Trp Asn Ser Ala Gly Glu Val Thr Asn Lys Asp Tyr Ser

515 520 525

Lys Val Met Ala Ile Leu Thr Gly Ser Ser Val Ile Phe Thr Phe Val

530 535 540

Leu Val Phe Val Gly His Glu Lys Phe His Arg Asp Leu Ser Ser Pro

545 550 555 560

His Leu Lys Ser Tyr Ile Glu Arg Val Asp Gln Thr Glu Glu Val Ala

565 570 575

Ala Met Thr Gly Ser Thr Ala Asn Ser Ile Ser Ser Lys Pro Ser Asp

580 585 590

Asp Gln Leu Glu Lys Val Ser Val

595 600

<210> 25

<211> 606

<212> PRT

<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)

<400> 25

Met His Thr Ile Ser Pro Glu Ser Asp Ser Leu Lys Ser Ser Lys Ser

1 5 10 15

Asn Thr Asn Ser His Glu Tyr Met Ser Ala Thr Thr Asp Tyr Asn Asn

20 25 30

Gly Gly Ala Ser Ala Ala Pro Leu Ser Ala Lys Arg Lys Glu Thr Gly

35 40 45

Asn Ser Tyr Glu Leu Gln Asp His Ala Ala Ser Ala Ser Ala Ser Ala

50 55 60

Ser Ala Ser Ala Ser Ala Ser Ala Ser Ala Ser Ala Leu Ala Phe Asp

65 70 75 80

Tyr Asp Pro Glu Asp Asp Asp Val Ser Ile Met Met Gln Asp Glu Glu

85 90 95

Val Pro Asp Leu Thr Trp Lys His Val Ser Asn Tyr Ala Ile Thr Arg

100 105 110

Phe Thr Ser Leu Ala His Ile His Pro Val Ser Met Gln Asn Ile Asn

115 120 125

Pro Phe Pro Glu Leu Arg Lys Met Thr Leu His Asn Trp Asn Tyr Phe

130 135 140

Phe Met Gly Tyr Gly Ala Trp Leu Cys Ala Ser Trp Ala Phe Phe Ala

145 150 155 160

Val Ser Val Ser Thr Ala Pro Leu Ala Glu Leu Tyr Asp Lys Gln Thr

165 170 175

Lys Asp Ile Ser Trp Gly Leu Ser Leu Val Leu Phe Val Arg Ser Ala

180 185 190

Gly Ala Ile Ile Phe Gly Ile Trp Thr Asp Asn Tyr Ser Arg Lys Trp

195 200 205

Pro Tyr Ile Thr Cys Leu Gly Leu Phe Leu Ile Cys Gln Leu Cys Thr

210 215 220

Pro Phe Ala Thr Asn Tyr Thr Gln Phe Leu Gly Val Arg Trp Ile Ser

225 230 235 240

Gly Val Ala Met Gly Gly Ile Tyr Ala Cys Ala Ser Ala Thr Ala Ile

245 250 255

Glu Asp Ala Pro Val Lys Ala Arg Ser Phe Leu Ser Gly Leu Phe Phe

260 265 270

Thr Ala Tyr Ala Met Gly Phe Ile Phe Ala Thr Val Phe Tyr Arg Ala

275 280 285

Phe Leu Asn Ile Asn Gly Lys Asp Tyr Trp Lys Ile Gln Phe Trp Phe

290 295 300

Ser Ile Trp Leu Pro Ala Ala Leu Ile Leu Trp Arg Leu Val Trp Pro

305 310 315 320

Glu Thr Arg Tyr Phe Thr Lys Val Leu Lys Ala Arg Gln Leu Met Leu

325 330 335

Asp Asp Ala Ile Ala Ala Asn Gly Gly Gln Pro Leu Pro Lys Lys Thr

340 345 350

Phe Gln Glu Lys Val Thr Asn Val Lys Lys Ile Ile Ser Thr Tyr Trp

355 360 365

Leu Leu Phe Thr Tyr Leu Ile Leu Leu Leu Val Gly Pro Asn Tyr Leu

370 375 380

Thr His Ala Ser Met Asp Leu Phe Pro Thr Met Leu Arg Ala Gln Leu

385 390 395 400

Lys Phe Ser Glu Asp Ala Val Thr Val Ala Ile Val Val Val Cys Ile

405 410 415

Gly Ala Ile Cys Gly Gly Met Phe Phe Gly Gln Leu Met Glu Ile Thr

420 425 430

Gly Arg Arg Leu Gly Leu Leu Ile Ala Leu Val Met Ala Gly Cys Phe

435 440 445

Thr Tyr Pro Ala Phe Met Leu Asn Thr Gly Pro Ala Val Leu Gly Gly

450 455 460

Gly Phe Phe Leu Trp Phe Ser Val Ile Gly Val Trp Gly Val Ile Pro

465 470 475 480

Ile His Leu Ser Glu Leu Ser Pro Pro Glu Ala Arg Ala Leu Val Ser

485 490 495

Gly Leu Ala Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ser Val Val

500 505 510

Ile Glu Ser Asp Leu Ala Asp Leu Tyr Pro Ile Ala Trp Asn Ala Ala

515 520 525

Gly Glu Val Thr Asn Lys Asn Tyr Ser Lys Val Met Ala Ile Leu Thr

530 535 540

Gly Ser Val Val Ile Phe Thr Phe Val Leu Val Leu Val Gly His Glu

545 550 555 560

Lys Phe His Arg Asp Leu Ser Ser Pro His Leu Lys Glu Tyr Thr Asp

565 570 575

Arg Val Asp Arg Ala Glu Glu Ala Ala Gly Met Gly Ser Ser Ala Ala

580 585 590

Ser Val Ser Ser Lys Pro Glu Gln Asn Phe Val Glu Lys Val

595 600 605

<210> 26

<211> 604

<212> PRT

<213> 马克斯克鲁维酵母(Kluyveromyces marxianus)

<400> 26

Met Ser Glu Gly Asn Glu Glu Lys Gly Gln Gly Gln Gly Gln Gly Gln

1 5 10 15

Asp His Gly His Phe Pro Glu Gly Arg Thr Phe Leu His Gln Val Glu

20 25 30

Ser Lys Arg Ser Val His Thr His His Ser Val Pro Met Asn Ser Ser

35 40 45

Asp Ser Gln Ser Asn Ala Ser Asp Pro Glu Glu Leu Ala Pro Phe Pro

50 55 60

Ser Val Val Asp Glu Ser Glu Leu Asp Glu Ser Glu Leu Glu Glu Ile

65 70 75 80

Gln Asn Glu Asp Val Ser Ile Ile Tyr Lys Asp Asp Glu Glu Lys Pro

85 90 95

Asp Leu Ser Trp Ala Ser Ile Cys Arg Tyr Ala Gly Thr Arg Ile Thr

100 105 110

Ser Leu Phe Gln Ile His Lys Val Ser Met Asp Asn Ile Asn Pro Phe

115 120 125

Pro Glu Leu Arg Lys Met Thr Leu Ser Asn Trp Asn Tyr Phe Phe Met

130 135 140

Gly Tyr Leu Ala Trp Leu Cys Ala Ala Trp Ala Phe Phe Ala Val Ser

145 150 155 160

Val Ser Thr Ala Pro Leu Ala Lys Leu Tyr Asp Lys Pro Thr Lys Asp

165 170 175

Ile Ser Trp Gly Leu Ser Leu Val Leu Phe Val Arg Ser Ala Gly Ala

180 185 190

Ile Ile Phe Gly Leu Trp Thr Asp Asn Tyr Ser Arg Lys Trp Pro Tyr

195 200 205

Ile Thr Cys Leu Gly Leu Phe Leu Ile Cys Gln Leu Cys Thr Pro Trp

210 215 220

Ala Lys Thr Tyr Thr Gln Phe Leu Gly Val Arg Trp Ile Ser Gly Ile

225 230 235 240

Ala Met Gly Gly Ile Tyr Gly Cys Ala Ser Ala Thr Ala Ile Glu Asp

245 250 255

Ala Pro Val Lys Ala Arg Ser Ile Leu Ser Gly Leu Phe Phe Ser Ala

260 265 270

Tyr Ala Met Gly Phe Ile Phe Ala Ile Ile Phe Tyr Arg Ala Phe Leu

275 280 285

His Val Asn Gly Glu Asn Tyr Trp Lys Val Gln Phe Trp Phe Ser Ile

290 295 300

Phe Leu Pro Ala Leu Leu Met Leu Trp Arg Leu Val Trp Pro Glu Thr

305 310 315 320

Lys Tyr Phe Thr Lys Val Leu Lys Ala Arg Glu Leu Met Lys Gln Asp

325 330 335

Ala Ile Ala Ser Asn Gly Gly Asn Pro Ile Pro Lys Thr Thr Phe Lys

340 345 350

Glu Lys Met Ala Asn Thr Lys Arg Ile Ile Cys Lys Tyr Trp Leu Leu

355 360 365

Phe Gly Tyr Leu Val Leu Leu Leu Val Gly Pro Asn Tyr Leu Thr His

370 375 380

Gly Ser Gln Asp Leu Phe Pro Thr Met Ile Arg Ser Gln Leu Asn Phe

385 390 395 400

Ser Glu Asp Ala Val Thr Val Ala Ile Val Val Val Asn Leu Gly Gly

405 410 415

Ile Cys Gly Gly Leu Phe Phe Gly Gln Leu Met Glu Val Thr Gly Arg

420 425 430

Arg Leu Gly Leu Leu Ile Ala Leu Thr Met Ala Gly Cys Phe Thr Tyr

435 440 445

Pro Ala Phe Met Leu Lys Thr Ser Ser Ala Val Leu Gly Ala Gly Phe

450 455 460

Met Leu Phe Phe Ser Val Phe Gly Val Trp Gly Val Ile Pro Ile His

465 470 475 480

Leu Ser Glu Leu Ser Pro Pro Glu Ala Arg Ala Leu Val Ala Gly Leu

485 490 495

Ala Tyr Gln Leu Gly Asn Leu Ala Ser Ala Ala Ser Val Val Ile Glu

500 505 510

Asn Asp Leu Ala Asp Ile Tyr Pro Leu Ala Arg Asp Ala Ala Gly Lys

515 520 525

Val Ile Lys Lys Asp Tyr Ala Lys Val Met Ala Ile Leu Thr Gly Ser

530 535 540

Val Val Ile Phe Thr Phe Val Met Val Leu Ile Gly His Glu Lys Phe

545 550 555 560

His Arg Asp Leu Ser Ser Ala Val Leu Lys Ala Tyr Met Glu Lys Val

565 570 575

Asp Arg Asn Glu Glu Asp Ala Arg Leu Gly Val Ser Ser Leu Ser Ile

580 585 590

Ser Ser Lys Ser Lys Glu Glu Gln Val Glu Asn Ala

595 600

<210> 27

<211> 591

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 27

Met Leu Lys Tyr Lys Pro Leu Leu Lys Ile Ser Lys Asn Cys Glu Ala

1 5 10 15

Ala Ile Leu Arg Ala Ser Lys Thr Arg Leu Asn Thr Ile Arg Ala Tyr

20 25 30

Gly Ser Thr Val Pro Lys Ser Lys Ser Phe Glu Gln Asp Ser Arg Lys

35 40 45

Arg Thr Gln Ser Trp Thr Ala Leu Arg Val Gly Ala Ile Leu Ala Ala

50 55 60

Thr Ser Ser Val Ala Tyr Leu Asn Trp His Asn Gly Gln Ile Asp Asn

65 70 75 80

Glu Pro Lys Leu Asp Met Asn Lys Gln Lys Ile Ser Pro Ala Glu Val

85 90 95

Ala Lys His Asn Lys Pro Asp Asp Cys Trp Val Val Ile Asn Gly Tyr

100 105 110

Val Tyr Asp Leu Thr Arg Phe Leu Pro Asn His Pro Gly Gly Gln Asp

115 120 125

Val Ile Lys Phe Asn Ala Gly Lys Asp Val Thr Ala Ile Phe Glu Pro

130 135 140

Leu His Ala Pro Asn Val Ile Asp Lys Tyr Ile Ala Pro Glu Lys Lys

145 150 155 160

Leu Gly Pro Leu Gln Gly Ser Met Pro Pro Glu Leu Val Cys Pro Pro

165 170 175

Tyr Ala Pro Gly Glu Thr Lys Glu Asp Ile Ala Arg Lys Glu Gln Leu

180 185 190

Lys Ser Leu Leu Pro Pro Leu Asp Asn Ile Ile Asn Leu Tyr Asp Phe

195 200 205

Glu Tyr Leu Ala Ser Gln Thr Leu Thr Lys Gln Ala Trp Ala Tyr Tyr

210 215 220

Ser Ser Gly Ala Asn Asp Glu Val Thr His Arg Glu Asn His Asn Ala

225 230 235 240

Tyr His Arg Ile Phe Phe Lys Pro Lys Ile Leu Val Asp Val Arg Lys

245 250 255

Val Asp Ile Ser Thr Asp Met Leu Gly Ser His Val Asp Val Pro Phe

260 265 270

Tyr Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Leu Glu Gly

275 280 285

Glu Lys Asp Val Ala Arg Gly Cys Gly Gln Gly Val Thr Lys Val Pro

290 295 300

Gln Met Ile Ser Thr Leu Ala Ser Cys Ser Pro Glu Glu Ile Ile Glu

305 310 315 320

Ala Ala Pro Ser Asp Lys Gln Ile Gln Trp Tyr Gln Leu Tyr Val Asn

325 330 335

Ser Asp Arg Lys Ile Thr Asp Asp Leu Val Lys Asn Val Glu Lys Leu

340 345 350

Gly Val Lys Ala Leu Phe Val Thr Val Asp Ala Pro Ser Leu Gly Gln

355 360 365

Arg Glu Lys Asp Met Lys Leu Lys Phe Ser Asn Thr Lys Ala Gly Pro

370 375 380

Lys Ala Met Lys Lys Thr Asn Val Glu Glu Ser Gln Gly Ala Ser Arg

385 390 395 400

Ala Leu Ser Lys Phe Ile Asp Pro Ser Leu Thr Trp Lys Asp Ile Glu

405 410 415

Glu Leu Lys Lys Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val Gln

420 425 430

Arg Thr Glu Asp Val Ile Lys Ala Ala Glu Ile Gly Val Ser Gly Val

435 440 445

Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala Pro

450 455 460

Ile Glu Val Leu Ala Glu Thr Met Pro Ile Leu Glu Gln Arg Asn Leu

465 470 475 480

Lys Asp Lys Leu Glu Val Phe Val Asp Gly Gly Val Arg Arg Gly Thr

485 490 495

Asp Val Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly

500 505 510

Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly Arg Asn Gly Val Glu

515 520 525

Lys Ala Ile Glu Ile Leu Arg Asp Glu Ile Glu Met Ser Met Arg Leu

530 535 540

Leu Gly Val Thr Ser Ile Ala Glu Leu Lys Pro Asp Leu Leu Asp Leu

545 550 555 560

Ser Thr Leu Lys Ala Arg Thr Val Gly Val Pro Asn Asp Val Leu Tyr

565 570 575

Asn Glu Val Tyr Glu Gly Pro Thr Leu Thr Glu Phe Glu Asp Ala

580 585 590

<210> 28

<211> 578

<212> PRT

<213> 东方伊萨酵母(Issatchenkia orientalis)

<400> 28

Met Leu Leu Arg Ser Leu Asn Ser Ser Ala Arg Cys Val Lys Gln Thr

1 5 10 15

Thr Arg Thr Lys Val Arg Tyr Leu Ser His Val Ser Gly Ala Ser Met

20 25 30

Ala Lys Pro Thr Leu Lys Asn Asn Ser Arg Glu Ser Asn Lys Ser Arg

35 40 45

Asn Tyr Leu Ile Ala Ala Val Thr Ala Leu Ala Val Ser Thr Ser Ile

50 55 60

Gly Val Ala Val His Val Lys Asp Pro Leu Tyr Asn Asp Ala Thr Gly

65 70 75 80

Ser Asp Ser Pro Arg Ser Ile Ser Val Asp Glu Phe Val Lys His Asn

85 90 95

Ser Gln Asn Asp Cys Trp Ile Ala Ile Asn Gly Lys Val Tyr Asp Phe

100 105 110

Thr Asp Phe Ile Pro Asn His Pro Gly Gly Val Pro Pro Leu Val Asn

115 120 125

His Ala Gly Tyr Asp Gly Thr Lys Leu Tyr Glu Lys Leu His Pro Lys

130 135 140

Gly Thr Ile Glu Lys Phe Leu Pro Lys Asp Lys Phe Leu Gly Val Leu

145 150 155 160

Asp Gly Glu Ala Pro Lys Leu Glu Ala Asp Tyr Leu Val Asp Asp Asp

165 170 175

Glu Gln Glu Arg Leu Asp Tyr Leu Asn Asn Leu Pro Pro Leu Ser Ser

180 185 190

Ile Gln Asn Val Tyr Asp Phe Glu Tyr Leu Ala Lys Lys Ile Leu Pro

195 200 205

Lys Asp Ala Trp Ala Tyr Tyr Ser Cys Gly Ala Asp Asp Glu Ile Thr

210 215 220

Met Arg Glu Asn His Tyr Ala Tyr Gln Arg Val Tyr Phe Arg Pro Arg

225 230 235 240

Ile Cys Val Asp Val Lys Glu Val Asp Thr Ser Tyr Glu Met Leu Gly

245 250 255

Thr Lys Thr Ser Val Pro Phe Tyr Val Ser Ala Thr Ala Leu Ala Lys

260 265 270

Leu Gly His Pro Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys

275 280 285

Glu Gly Val Val Gln Met Ile Ser Thr Leu Ser Ser Met Ser Leu Asp

290 295 300

Glu Ile Ala Ala Ala Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu

305 310 315 320

Tyr Ile Asn Glu Asp Arg Asn Val Ala Lys Gly Leu Val Lys His Ala

325 330 335

Glu Asp Leu Gly Met Lys Ala Ile Phe Ile Thr Val Asp Ala Pro Ser

340 345 350

Leu Gly Asn Arg Glu Lys Asp Lys Arg Leu Lys Phe Val Asn Asp Thr

355 360 365

Asp Val Asp Leu Gly Asp Ser Ala Asp Arg Asn Ser Gly Ala Ser Lys

370 375 380

Ala Leu Ser Ser Phe Ile Asp Ala Ser Val Ser Trp Asn Asp Val Lys

385 390 395 400

Ala Val Lys Ser Trp Thr Lys Leu Pro Val Leu Val Lys Gly Val Gln

405 410 415

Thr Val Glu Asp Val Ile Glu Ala Tyr Asp Ala Gly Cys Gln Gly Val

420 425 430

Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ala Pro Pro Pro

435 440 445

Ile Glu Leu Leu Ala Glu Thr Val Pro Thr Leu Lys Arg Leu Gly Lys

450 455 460

Leu Arg Pro Asp Phe Glu Ile Leu Ile Asp Gly Gly Val Lys Arg Gly

465 470 475 480

Thr Asp Ile Leu Lys Ala Val Ala Ile Gly Gly Gln Asp Val Arg Val

485 490 495

Ser Val Gly Met Gly Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly

500 505 510

Glu Ala Gly Val Arg Lys Leu Ile Gln Asn Leu Lys Asp Glu Leu Glu

515 520 525

Met Asp Met Arg Leu Leu Gly Val Thr Lys Met Asp Gln Leu Ser Ser

530 535 540

Lys His Val Asp Thr Lys Arg Leu Ile Gly Arg Asp Ala Ile Asn Tyr

545 550 555 560

Leu Tyr Asp Asn Val Tyr Ser Pro Ile Glu Thr Val Lys Phe Asn Asn

565 570 575

Glu Asp

<210> 29

<211> 423

<212> PRT

<213> 克鲁弗酵母(Saccharomyces kluyveri)

<400> 29

Met Pro Gln Asp Leu Val Cys Ser Pro Leu Thr Pro Gly Glu Thr Pro

1 5 10 15

Asp Asp Ile Val Arg Lys Glu Glu Leu Arg Ser Arg Leu Pro Ser Leu

20 25 30

Asp Ala Leu Val Asn Leu Tyr Asp Phe Glu Tyr Leu Ala Ser Gln Ile

35 40 45

Leu Thr Lys Gln Ala Trp Ser Tyr Tyr Ser Ser Ala Ala Asp Asp Glu

50 55 60

Val Thr His Arg Glu Asn His Ala Ala Tyr His Arg Ile Phe Phe Lys

65 70 75 80

Pro Lys Val Leu Val Asp Val Arg Glu Val Asp Thr Ser Thr Lys Ile

85 90 95

Leu Gly Gln Asp Val Pro Val Pro Phe Tyr Val Ser Ala Thr Ala Leu

100 105 110

Cys Lys Leu Gly Asn Pro Lys Glu Gly Glu Lys Asp Val Ala Arg Gly

115 120 125

Cys Gly Gln Gly Pro Ile Lys Cys Pro Gln Met Ile Ser Thr Leu Ala

130 135 140

Ser Cys Ser Leu Gln Glu Ile Val Glu Ala Ala Pro Ser Lys Glu Gln

145 150 155 160

Ile Gln Trp Phe Gln Leu Tyr Val Asn Thr Asp Arg Lys Ile Thr Asp

165 170 175

Asp Leu Ile Lys Gln Val Glu Lys Leu Gly Leu Lys Ala Ile Phe Val

180 185 190

Thr Val Asp Ala Pro Ser Leu Gly Arg Arg Glu Lys Asp Met Lys Val

195 200 205

Lys Phe Thr Asn Ser Asn Gly Ala Lys Ala Met Glu Lys Thr Lys Val

210 215 220

Lys Glu Ser Lys Gly Ala Ser Ala Ala Leu Ser Thr Phe Ile Asp Pro

225 230 235 240

Ala Leu Ser Trp Asp Asp Ile Val Glu Ile Lys Lys Lys Thr Asn Leu

245 250 255

Pro Ile Val Ile Lys Gly Val Gln Arg Val Glu Asp Val Leu Lys Ala

260 265 270

Ala Glu Val Gly Ala Ala Gly Val Val Leu Ser Asn His Gly Gly Arg

275 280 285

Gln Leu Asp Phe Ser Arg Ala Pro Ile Glu Val Leu Ala Glu Ser Val

290 295 300

Pro Val Leu Lys Glu Arg Asn Leu Ser Asp Lys Leu Glu Ile Phe Ile

305 310 315 320

Asp Gly Gly Val Arg Arg Gly Asn Asp Val Leu Lys Ala Leu Cys Leu

325 330 335

Gly Ala Lys Gly Val Gly Leu Gly Arg Pro Phe Leu Tyr Ala Asn Ser

340 345 350

Cys Tyr Gly Lys Asp Gly Val Gln Lys Ala Ile Glu Met Leu Thr Glu

355 360 365

Glu Ile Gln Met Ser Met Arg Leu Leu Gly Val Thr Lys Ile Glu Asp

370 375 380

Leu Lys Pro Glu Leu Leu Asp Met Ser Ser Phe Ser Gly Arg Thr Val

385 390 395 400

Asn Val Pro Gln Asp Ser Leu Tyr Arg Ser Val Tyr Glu Lys Ser Glu

405 410 415

Thr Val Gln Phe Leu Asp Glu

420

<210> 30

<211> 591

<212> PRT

<213> 贝酵母(Saccharomyces bayanus)

<400> 30

Met Leu Lys Tyr Lys Ser Leu Leu Arg Ile Ser Arg Val Ser Glu Ala

1 5 10 15

Ala Ala Leu Arg Thr Ser Arg Thr Arg Leu Asn Thr Val Arg Ser Tyr

20 25 30

Ser Ser Ala Val Gly Lys Ser Lys Ser Phe Glu Lys His Ser Gly Lys

35 40 45

Arg Thr Gln Ser Trp Thr Ala Leu Ser Val Gly Ala Ile Leu Ala Ala

50 55 60

Ala Gly Ser Met Ala Tyr Val Asn Trp Gly Ser Gly Gln Ile Gly Asn

65 70 75 80

Asp Pro Lys Leu Asp Met Asn Lys Gln Lys Ile Ser Pro Ala Glu Val

85 90 95

Ala Lys His Asn Ser Ala Asp Asp Cys Trp Val Val Ile Asn Gly Tyr

100 105 110

Val Tyr Asp Leu Thr Arg Phe Val Pro Asn His Pro Gly Gly Pro Asp

115 120 125

Val Ile Lys Tyr Asn Ala Gly Arg Asp Val Thr Ala Ile Phe Glu Pro

130 135 140

Leu His Ala Pro Asn Val Ile Asp Lys Tyr Ile Ala Pro Glu Lys Lys

145 150 155 160

Leu Gly Pro Leu Gln Gly Ser Met Pro Pro Glu Leu Val Cys Pro Pro

165 170 175

Tyr Ala Pro Gly Glu Thr Lys Glu Asp Ile Ala Arg Lys Glu Gln Leu

180 185 190

Lys Ser Leu Leu Pro Pro Leu Asp Asn Ile Ile Asn Leu Tyr Asp Phe

195 200 205

Glu Tyr Leu Ala Ser Gln Thr Leu Thr Arg Gln Ala Trp Ala Tyr Tyr

210 215 220

Ser Ser Gly Ser Asn Asp Glu Val Ser His Arg Glu Asn His Asn Ala

225 230 235 240

Tyr His Arg Ile Phe Phe Lys Pro Lys Ile Leu Val Asp Val Ser Lys

245 250 255

Val Asp Ile Ser Thr Asp Met Leu Gly Ser His Val Asp Val Pro Phe

260 265 270

Tyr Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Leu Glu Gly

275 280 285

Glu Lys Asp Ile Ala Arg Gly Cys Gly Gln Gly Ala Thr Lys Val Pro

290 295 300

Gln Met Ile Ser Thr Leu Ala Ser Cys Ser Pro Glu Glu Ile Ile Ser

305 310 315 320

Ala Ala Pro Ser Asp Glu Gln Ile Gln Trp Tyr Gln Leu Tyr Val Asn

325 330 335

Ser Asp Arg Lys Ile Thr Asp Glu Leu Val Lys Ser Val Glu Lys Leu

340 345 350

Gly Val Lys Ala Leu Phe Val Thr Val Asp Ala Pro Ser Leu Gly Gln

355 360 365

Arg Glu Lys Asp Met Lys Leu Lys Phe Ser Asn Ser Lys Ala Gly Pro

370 375 380

Lys Ala Met Lys Lys Thr Asp Val Glu Glu Ser Lys Gly Ala Ser Arg

385 390 395 400

Ala Leu Ser Lys Phe Ile Asp Pro Ser Leu Thr Trp Lys Asp Ile Glu

405 410 415

Glu Leu Lys Ser Lys Thr Asn Leu Pro Ile Val Ile Lys Gly Val Gln

420 425 430

Arg Thr Glu Asp Val Val Lys Ala Ala Glu Val Gly Val Ser Gly Val

435 440 445

Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala Pro

450 455 460

Ile Glu Val Leu Ala Glu Ala Met Pro Val Leu Glu Glu Arg Lys Leu

465 470 475 480

Lys Asp Lys Leu Glu Val Tyr Ile Asp Gly Gly Val Arg Arg Gly Thr

485 490 495

Asp Ile Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly

500 505 510

Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly Arg Asp Gly Val Glu

515 520 525

Lys Ala Ile Glu Ile Leu Arg Asp Glu Val Glu Met Ser Met Arg Leu

530 535 540

Leu Gly Val Ala Ser Val Ala Glu Leu Lys Pro Glu Leu Leu Asp Leu

545 550 555 560

Ser Thr Leu Lys Ala Arg Thr Val Ala Val Pro Asn Asp Val Leu Tyr

565 570 575

Asn Glu Val Tyr Glu Gly Pro Thr Leu Thr Asp Phe Glu Asp Ala

580 585 590

<210> 31

<211> 598

<212> PRT

<213> 鲁氏接合酵母(Zygosaccharomyces rouxii)

<400> 31

Met Trp Phe Arg Ser Lys Pro Ile Trp Lys Leu Ser Glu Val Thr Ala

1 5 10 15

Arg Ser Leu Arg Gly Ile Asn Thr Lys Arg Ser Phe Thr Trp Asn Ser

20 25 30

Lys Gly Ser Asn Ala Phe Val Arg Ser Ser Ile Leu Lys Lys Arg Ser

35 40 45

Met Ala Thr Ala Leu Leu Val Thr Ala Val Ala Ala Ala Ser Ser Val

50 55 60

Lys Leu Ser Ser Arg Pro Leu Asp Asn Gly Pro Lys Val Asp Met Ser

65 70 75 80

Lys Pro Ala Ile Ser Pro Asp Glu Val Ala Lys His Asn Ser Pro Asp

85 90 95

Asp Cys Trp Val Val Ile Asp Gly Tyr Val Tyr Asp Leu Thr Glu Phe

100 105 110

Ala Pro Val His Pro Gly Gly Pro Thr Val Ile Lys Ser Asn Ala Gly

115 120 125

Lys Asp Val Ser Ala Ile Phe Asp Pro Leu His Ala Pro Asp Val Ile

130 135 140

Glu Lys Phe Ile Asp Pro Ser Lys Arg Leu Gly Pro Leu Glu Gly Asp

145 150 155 160

Met Pro Lys Glu Leu Val Cys Ala Pro Phe Thr Pro Gly Glu Thr Pro

165 170 175

Asp Asp Val Gln Arg Lys Leu Lys Leu Arg Ala Arg Leu Pro Pro Leu

180 185 190

Asp Ala Ile Thr Asn Leu Tyr Asp Phe Glu Phe Leu Ala Ser Gln Val

195 200 205

Leu Thr Lys Gln Ala Trp Ala Tyr Tyr Ser Ser Gly Ala Asp Asp Glu

210 215 220

Ile Thr Met Arg Glu Asn His Phe Ala Tyr His Arg Ile Phe Phe Lys

225 230 235 240

Pro Lys Val Leu Val Asn Val Ala Glu Val Asp Thr Lys Thr Glu Met

245 250 255

Leu Gly Ala Pro Val Asp Val Pro Phe Tyr Val Thr Ala Thr Ala Leu

260 265 270

Cys Lys Leu Gly Asn Pro Ala Glu Gly Glu Lys Asp Ile Ala Arg Gly

275 280 285

Cys Gly Ser Gly Glu Lys Lys Val Pro Gln Met Val Ser Thr Leu Ala

290 295 300

Ser Cys Ser Leu Glu Glu Val Val Asn Ala Gly Lys Glu Asp Gln Ile

305 310 315 320

Arg Trp Phe Gln Leu Tyr Met Asn Glu Asp Arg Ser Val Val Asp Gln

325 330 335

Met Ile Ser Ser Ala Glu Lys Leu Gly Tyr Lys Gly Ile Phe Val Thr

340 345 350

Val Asp Ala Pro Gly Leu Gly Asn Arg Glu Lys Asp Thr Lys Val Lys

355 360 365

Phe Ser Ser Gln Ala Gly Pro Leu Ser Val Lys Lys Lys Glu Lys Glu

370 375 380

Asp Lys Gly Lys Asp Asn Gly Glu Ser Ser Gly Ala Ser Lys Tyr Leu

385 390 395 400

Ser Lys Phe Ile Asp Pro Ser Phe Asp Trp Asp Asp Leu Val Glu Val

405 410 415

Lys Lys Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val Gln Arg Val

420 425 430

Glu Asp Val Val Lys Ala Ala Glu Val Gly Ala Ser Gly Val Val Leu

435 440 445

Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ser Pro Ile Glu

450 455 460

Val Leu Ala Glu Ala Gln Pro Ile Leu Lys Glu Arg Asn Phe Glu Asn

465 470 475 480

Phe Asp Val Phe Val Asp Gly Gly Ile Arg Arg Gly Thr Asp Val Val

485 490 495

Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly Arg Pro Phe

500 505 510

Leu Tyr Ala Asn Ser Val Tyr Gly Lys Glu Gly Val Gln Lys Ala Ile

515 520 525

Asp Ile Leu Asn Phe Glu Val Glu Met Thr Met Arg Leu Leu Gly Val

530 535 540

Thr Ser Ile Lys Gln Leu Gly Pro Glu Leu Ile Asp Thr Ser Cys Ile

545 550 555 560

Lys Ser Arg Ser Val Gln Val Pro Arg Asp Tyr Leu Tyr Asp Lys Thr

565 570 575

Tyr Met Ser Ser Asp Leu Val Ser Phe Met Pro Pro Gln Glu Asp Glu

580 585 590

Ala Asp Gly Glu Asn Glu

595

<210> 32

<211> 589

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 32

Met Arg Ser Ala Val Arg Val Phe Gly Lys Asn Arg Val Ser Gly Leu

1 5 10 15

Arg Phe Ala Ser Thr Ser Ser Lys Ser Pro Ile His Lys Ser Ser Ala

20 25 30

Thr Leu Arg Phe Gly Arg Ser Ala Lys Ser Gly Tyr Ser Ala Phe Ser

35 40 45

Phe Gly Lys Lys Val Ile Thr Gly Gly Ala Phe Leu Val Ala Gly Ser

50 55 60

Val Gly Ile Ala Leu Ile Ser Gln Phe Asn Asp Ser Val Glu Asn Gly

65 70 75 80

Ala Lys Val Asp Met Thr Lys Pro Lys Val Ser Pro Thr Glu Val Ala

85 90 95

Lys His Ser Ser Pro Lys Asp Cys Trp Val Val Ile Glu Gly Tyr Val

100 105 110

Tyr Asn Leu Thr Asp Phe Ile Ser Ala His Pro Gly Gly Pro Ala Ile

115 120 125

Ile Glu Asn Asn Ala Gly Lys Asp Val Thr Lys Ile Phe Ala Pro Ile

130 135 140

His Ala Pro Asp Val Ile Glu Lys Tyr Ile Ala Pro Glu Asn Arg Ile

145 150 155 160

Gly Pro Leu Asp Gly Thr Met Pro Asp Asp Leu Val Cys Ala Ala Leu

165 170 175

Thr Pro Gly Glu Thr Pro Glu Asp Val Ala Arg Lys Glu Gln Leu Arg

180 185 190

Gln Ser Leu Pro Asp Ile Asp Ser Leu Val Asn Ile Tyr Asp Phe Glu

195 200 205

Phe Leu Ala Ser Gln Ile Leu Thr Lys Gln Ala Trp Ser Tyr Tyr Ser

210 215 220

Ser Ala Ala Asp Asp Glu Val Thr His Arg Glu Asn His Ala Ala Tyr

225 230 235 240

His Arg Ile Phe Phe Lys Pro Arg Ile Leu Val Asn Val Lys Glu Val

245 250 255

Asp Thr Ser Thr Thr Met Leu Gly Glu Lys Val Gly Val Pro Phe Tyr

260 265 270

Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Lys Glu Gly Glu

275 280 285

Lys Asp Ile Ala Arg Gly Cys Gly Glu Ser Asp Val Lys Pro Val Gln

290 295 300

Met Ile Ser Thr Leu Ala Ser Cys Ser Leu Gln Glu Ile Val Glu Ala

305 310 315 320

Ala Pro Ser Lys Glu Gln Ile Gln Trp Phe Gln Leu Tyr Val Asn Ser

325 330 335

Asp Arg Lys Ile Thr Glu Asp Leu Ile Lys Asn Val Glu Lys Leu Gly

340 345 350

Leu Lys Ala Ile Phe Val Thr Val Asp Ala Pro Ser Leu Gly Asn Arg

355 360 365

Glu Lys Asp Ala Lys Val Lys Phe Thr Asn Ser Asn Gly Ala Lys Ala

370 375 380

Met Glu Lys Ser Lys Val Lys Glu Ser Lys Gly Ala Ser Arg Ala Leu

385 390 395 400

Ser Ser Phe Ile Asp Pro Ala Leu Asn Trp Asp Asp Ile Ile Glu Phe

405 410 415

Lys Lys Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val Gln Cys Val

420 425 430

Glu Asp Val Leu Lys Ala Ala Glu Ile Gly Val Ala Gly Val Val Leu

435 440 445

Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala Pro Ile Glu

450 455 460

Val Leu Ala Glu Thr Met Pro Val Leu Arg Glu Lys Lys Leu Asp Asp

465 470 475 480

Lys Ile Glu Ile Phe Ile Asp Gly Gly Val Arg Arg Gly Thr Asp Ile

485 490 495

Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu Gly Arg Pro

500 505 510

Phe Leu Tyr Ser Asn Ser Cys Tyr Gly Lys Glu Gly Val Lys Lys Ala

515 520 525

Ile Glu Leu Leu Lys Asp Glu Leu Glu Met Ser Met Arg Leu Leu Gly

530 535 540

Val Thr Ser Ile Asp Gln Leu Ser Glu Lys Tyr Leu Asp Leu Ser Thr

545 550 555 560

Ile His Gly Arg Thr Val Asn Val Pro Arg Asp Asn Leu Tyr Arg Glu

565 570 575

Val Tyr Val Pro His Glu Pro Thr Gln Phe Leu Glu Glu

580 585

<210> 33

<211> 592

<212> PRT

<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)

<400> 33

Met Arg Ser Ala Val Arg Val Phe Arg Lys Asn Arg Phe Pro Thr Gly

1 5 10 15

Leu Arg Leu Ser Ser Thr Ala Ser Ser Ala Arg Gln Gly Ile Ala Leu

20 25 30

Ser Val Gly Lys Tyr Asn Lys Tyr Asn Lys Asn Gly Thr Asn Gly Ser

35 40 45

Trp Lys Ser Ser Arg Lys Ser Thr Thr Tyr Gly Gly Ala Phe Leu Val

50 55 60

Ala Ser Ala Val Ala Ile Ala Phe Ser Ser Gln Ile Thr Gln Ser Leu

65 70 75 80

Glu Asn Gly Val Lys Val Asp Met Gly Lys Pro Ser Val Ser Pro Ala

85 90 95

Glu Val Val Lys His Ser Ser Pro Asp Asp Cys Trp Val Val Ile Asp

100 105 110

Gly Tyr Val Tyr Asn Leu Thr Glu Phe Ile Gly Ala His Pro Gly Gly

115 120 125

Pro Ala Ile Ile Lys Asn Asn Ala Gly Lys Asp Val Thr Lys Ile Phe

130 135 140

Ala Pro Ile His Ala Pro Asp Val Ile Glu Lys Tyr Ile Ala Pro Lys

145 150 155 160

Asn Arg Leu Gly Pro Leu Glu Gly Thr Met Pro Glu Asp Arg Ile Cys

165 170 175

Ala Ala Leu Thr Pro Gly Glu Thr Ala Glu Asp Val Ala Arg Lys Glu

180 185 190

Gln Leu Arg Gln Leu Leu Pro Asp Val Asn Asn Leu Thr Asn Leu Tyr

195 200 205

Asp Phe Glu Phe Leu Ala Ser Gln Ile Leu Thr Lys Gln Ala Trp Ser

210 215 220

Tyr Tyr Ser Ser Ala Ala Asp Asp Glu Val Thr Gln Arg Glu Asn His

225 230 235 240

Ala Ala Tyr His Arg Ile Phe Phe Lys Pro Arg Ile Leu Val Asn Val

245 250 255

Lys Glu Val Asp Thr Ser Thr Thr Met Leu Gly Glu Lys Val Gly Val

260 265 270

Pro Phe Tyr Val Ser Ala Thr Ala Leu Cys Lys Leu Gly Asn Pro Gln

275 280 285

Glu Gly Glu Lys Asp Ile Ala Arg Gly Cys Gly Glu Ser Asp Ala Lys

290 295 300

Pro Val Gln Met Ile Ser Thr Leu Ala Ser Cys Ser Leu Gln Glu Ile

305 310 315 320

Val Glu Ala Ala Pro Ser Lys Glu Gln Ile Gln Trp Phe Gln Leu Tyr

325 330 335

Val Asn Ser Asp Arg Lys Ile Thr Asp Asp Leu Val Lys Asn Ala Glu

340 345 350

Lys Leu Gly Leu Lys Ala Ile Phe Val Thr Val Asp Ala Pro Ser Leu

355 360 365

Gly Asn Arg Glu Lys Asp Ala Lys Val Lys Phe Thr Asn Ser Asn Gly

370 375 380

Ala Lys Ala Met Glu Lys Ser Asn Val Asp Glu Ser Lys Gly Ala Ser

385 390 395 400

Arg Ala Leu Ser Ser Phe Ile Asp Pro Ala Leu Asn Trp Asp Asp Ile

405 410 415

Val Glu Leu Lys Thr Lys Thr Lys Leu Pro Ile Val Ile Lys Gly Val

420 425 430

Gln Cys Val Glu Asp Val Leu Lys Ala Ala Glu Val Gly Ala Ala Gly

435 440 445

Val Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Phe Ser Arg Ala

450 455 460

Pro Ile Glu Val Leu Ala Glu Thr Met Pro Ala Leu Arg Glu Lys Lys

465 470 475 480

Leu Asp Asp Lys Leu Glu Val Phe Ile Asp Gly Gly Val Arg Arg Gly

485 490 495

Thr Asp Ile Leu Lys Ala Leu Cys Leu Gly Ala Lys Gly Val Gly Leu

500 505 510

Gly Arg Pro Phe Leu Tyr Ser Asn Ser Cys Tyr Gly Lys Glu Gly Val

515 520 525

Lys Lys Ala Ile Glu Leu Leu Lys Asp Glu Leu Glu Met Ser Met Arg

530 535 540

Leu Leu Gly Val Thr Ser Ile Asp Gln Leu Ser Glu Lys Tyr Leu Asp

545 550 555 560

Leu Ser Thr Ile His Gly Arg Thr Val Ser Val Pro Arg Asp Asn Ile

565 570 575

Tyr Arg Asp Val Tyr Val Ala Gln Glu Pro Ser Gln Phe Arg Glu Asp

580 585 590

<210> 34

<211> 587

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 34

Met Leu Trp Lys Arg Thr Cys Thr Arg Leu Ile Lys Pro Ile Ala Gln

1 5 10 15

Pro Arg Gly Arg Leu Val Arg Arg Ser Cys Tyr Arg Tyr Ala Ser Thr

20 25 30

Gly Thr Gly Ser Thr Asp Ser Ser Ser Gln Trp Leu Lys Tyr Ser Val

35 40 45

Ile Ala Ser Ser Ala Thr Leu Phe Gly Tyr Leu Phe Ala Lys Asn Leu

50 55 60

Tyr Ser Arg Glu Thr Lys Glu Asp Leu Ile Glu Lys Leu Glu Met Val

65 70 75 80

Lys Lys Ile Asp Pro Val Asn Ser Thr Leu Lys Leu Ser Ser Leu Asp

85 90 95

Ser Pro Asp Tyr Leu His Asp Pro Ala Lys Ile Asp Lys Val Val Glu

100 105 110

Asp Leu Lys Gln Val Leu Gly Asn Lys Pro Glu Asn Tyr Ser Asp Ala

115 120 125

Lys Ser Asp Leu Asp Ala His Ser Asp Thr Tyr Phe Asn Thr His His

130 135 140

Pro Ser Leu Glu Gln Arg Pro Arg Ile Ile Leu Phe Pro His Thr Thr

145 150 155 160

Glu Glu Val Ser Lys Ile Leu Lys Ile Cys His Asp Asn Asn Met Pro

165 170 175

Val Val Pro Phe Ser Gly Gly Thr Ser Leu Glu Gly His Phe Leu Pro

180 185 190

Thr Arg Ile Gly Asp Thr Ile Thr Val Asp Leu Ser Lys Phe Met Asn

195 200 205

Asn Val Val Lys Phe Asp Lys Leu Asp Leu Asp Ile Thr Val Gln Ala

210 215 220

Gly Leu Pro Trp Glu Asp Leu Asn Asp Tyr Leu Ser Asp His Gly Leu

225 230 235 240

Met Phe Gly Cys Asp Pro Gly Pro Gly Ala Gln Ile Gly Gly Cys Ile

245 250 255

Ala Asn Ser Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Lys

260 265 270

Glu Asn Ile Ile Asn Met Thr Ile Val Leu Pro Asp Gly Thr Ile Val

275 280 285

Lys Thr Lys Lys Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asn Leu Asn

290 295 300

Gly Leu Phe Val Gly Ser Glu Gly Thr Leu Gly Ile Val Thr Glu Ala

305 310 315 320

Thr Val Lys Cys His Val Lys Pro Lys Ala Glu Thr Val Ala Val Val

325 330 335

Ser Phe Asp Thr Ile Lys Asp Ala Ala Ala Cys Ala Ser Asn Leu Thr

340 345 350

Gln Ser Gly Ile His Leu Asn Ala Met Glu Leu Leu Asp Glu Asn Met

355 360 365

Met Lys Leu Ile Asn Ala Ser Glu Ser Thr Asp Arg Cys Asp Trp Val

370 375 380

Glu Lys Pro Thr Met Phe Phe Lys Ile Gly Gly Arg Ser Pro Asn Ile

385 390 395 400

Val Asn Ala Leu Val Asp Glu Val Lys Ala Val Ala Gln Leu Asn His

405 410 415

Cys Asn Ser Phe Gln Phe Ala Lys Asp Asp Asp Glu Lys Leu Glu Leu

420 425 430

Trp Glu Ala Arg Lys Val Ala Leu Trp Ser Val Leu Asp Ala Asp Lys

435 440 445

Ser Lys Asp Lys Ser Ala Lys Ile Trp Thr Thr Asp Val Ala Val Pro

450 455 460

Val Ser Gln Phe Asp Lys Val Ile His Glu Thr Lys Lys Asp Met Gln

465 470 475 480

Ala Ser Lys Leu Ile Asn Ala Ile Val Gly His Ala Gly Asp Gly Asn

485 490 495

Phe His Ala Phe Ile Val Tyr Arg Thr Pro Glu Glu His Glu Thr Cys

500 505 510

Ser Gln Leu Val Asp Arg Met Val Lys Arg Ala Leu Asp Ala Glu Gly

515 520 525

Thr Cys Thr Gly Glu His Gly Val Gly Ile Gly Lys Arg Glu Tyr Leu

530 535 540

Leu Glu Glu Leu Gly Glu Ala Pro Val Asp Leu Met Arg Lys Ile Lys

545 550 555 560

Leu Ala Ile Asp Pro Lys Arg Ile Met Asn Pro Asp Lys Ile Phe Lys

565 570 575

Thr Asp Pro Asn Glu Pro Ala Asn Asp Tyr Arg

580 585

<210> 35

<211> 533

<212> PRT

<213> 东方伊萨酵母(Issatchenkia orientalis)

<400> 35

Met Ser Arg Leu Phe Lys Val Leu Lys Ala Thr Gln Phe Lys Tyr Val

1 5 10 15

Ser Ser Phe Leu Leu Gly Ser Thr Ile Ser Gly Gly Val Val Tyr Tyr

20 25 30

Asn Tyr Lys Lys Ser Leu Asn Phe Ser Phe Pro Pro Thr Ser Thr Thr

35 40 45

Pro Ile Thr Ala Ala Pro Pro Leu Gln Tyr Gly Asp Val Ser Lys Ala

50 55 60

Ile Ser Glu Ile Thr Ser Lys Leu Gly Pro Ser Lys Ile Thr Phe Ser

65 70 75 80

Glu Ser Glu Ile Leu His His Ser Asp Ser Ser Phe Ser Thr Asp His

85 90 95

Ala Lys Pro His Glu Arg Pro His Ala Val Val Tyr Pro Glu Ser Thr

100 105 110

Glu Asp Val Ser Glu Val Leu Lys Ile Cys His Thr Leu Lys Val Pro

115 120 125

Val Val Pro Phe Thr Gly Gly Thr Ser Leu Glu Gly His Phe Ile Ser

130 135 140

Thr Arg Asn Gly Ile Ser Val Asp Leu Ser Arg Met Asn Lys Val Leu

145 150 155 160

Ala Ile His Pro Glu Asp Leu Asp Ala Val Val Gln Pro Ala Val Gly

165 170 175

Trp Glu Glu Leu Arg Asp Thr Leu Ser Asp Tyr Asn Leu Leu Phe Gly

180 185 190

Pro Asp Pro Gly Pro Gly Ala Cys Ile Gly Gly Met Val Ala Thr Ser

195 200 205

Cys Ser Gly Thr Asn Ala Ala Arg Tyr Gly Thr Met Arg Glu Asn Val

210 215 220

Val Ser Val Lys Val Val Leu Pro Asp Gly Thr Ile Val Lys Thr Lys

225 230 235 240

Arg Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asn Leu Thr Asn Leu Phe

245 250 255

Ile Gly Ser Glu Gly Thr Leu Gly Ile Val Thr Glu Val Thr Leu Lys

260 265 270

Leu Asn Val Lys Pro Lys Tyr Glu Ser Val Ala Leu Val Ser Phe Asp

275 280 285

Lys Leu Lys Asp Ala Ala Gly Ser Val Thr Ser Phe Val Gln Glu Gly

290 295 300

Leu Gln Leu Asn Ala Ile Glu Leu Leu Asp Asp Lys Met Val His Phe

305 310 315 320

Val Asn Glu Ser Gly Glu Thr Asp Leu Lys Tyr Asp Glu Leu Pro Thr

325 330 335

Leu Leu Leu Lys Ile Gly Gly Ser Ser Pro Glu Ser Val Asn Leu Leu

340 345 350

Ile Lys Thr Val Glu Arg Ile Val Asn Ala Asn Asn Ala Lys Thr Phe

355 360 365

Lys Phe Ala Ser Asn Glu Glu Glu Lys Tyr Gln Leu Trp Asn Ala Arg

370 375 380

Lys Thr Ala Leu Trp Ser Thr Ile Glu Tyr Gly Arg Arg Asn Ile Asp

385 390 395 400

Lys Asp Ile Gln Val Trp Thr Thr Asp Val Ala Val Pro Met Ser Lys

405 410 415

Phe Val Glu Ala Val Glu Glu Thr Gln Lys Glu Ile Glu Glu Ser Asp

420 425 430

Leu Leu Ala Ser Ile Val Gly His Ala Gly Asp Gly Asn Tyr His Thr

435 440 445

Val Ile Leu Phe Lys Glu Glu Gln Arg Ala Leu Ala Ala Lys Leu Val

450 455 460

Ser Asn Met Val Asp Arg Ala Leu Ala Phe Asp Gly Thr Val Ser Gly

465 470 475 480

Glu His Gly Ile Gly Val Gly Lys Lys Glu Phe Leu Ile Asp Glu Val

485 490 495

Gly Gln Glu Ser Val Asp Leu Met Arg Lys Ile Lys Phe Ser Ile Asp

500 505 510

Pro His Lys Ile Leu Asn Pro Asp Lys Val Phe Ser Ile Asp Pro Val

515 520 525

Asn Asp Arg Ser Ile

530

<210> 36

<211> 564

<212> PRT

<213> 克鲁弗酵母(Saccharomyces kluyveri)

<400> 36

Met Phe Lys Arg Ala Phe Leu Arg Pro Gln Leu Trp Ser Ser Thr Thr

1 5 10 15

Arg Arg Cys Thr Ile Ala Ser Ile Arg Cys Tyr Ser Lys Gln Pro Leu

20 25 30

Lys Pro Ala Ser Ala Ser Lys Leu Thr Leu Gly Leu Ile Gly Gly Ile

35 40 45

Gly Leu Gly Ser Ala Ala Thr Ala Tyr Gly Leu Leu Met Gly Asp Asp

50 55 60

Asp Thr Ser Ala Ser Thr Thr Arg Leu Asp Glu Leu Ala Pro Pro Ser

65 70 75 80

Tyr Cys Gln Asp Pro Lys Lys Phe Glu Asp Ala Ile Ile Lys Leu Lys

85 90 95

His Ile Leu Asp Asp Asn Pro Asp Asn Phe Ser Val Ser Lys Ser Glu

100 105 110

Leu Asp Ala His Ser Asp Thr Tyr Phe Asn Ser His His Ala Arg Pro

115 120 125

Asp Gln Arg Pro Gln Ile Ile Leu Phe Pro Arg Thr Thr Ser Asp Val

130 135 140

Ser Glu Ile Leu Lys Val Cys His Glu Tyr Glu Val Pro Val Ile Pro

145 150 155 160

Phe Ser Gly Gly Thr Ser Leu Glu Gly His Phe Leu Pro Thr Arg Ile

165 170 175

Gly Cys Thr Val Val Leu Asp Met Ser Lys Tyr Met Asn Lys Ile Leu

180 185 190

Lys Leu Asn Lys Gln Asp Leu Asp Val Glu Val Gln Ala Gly Val Pro

195 200 205

Trp Glu Asp Leu Asn Asp Tyr Leu Asn Ser Glu Gly Val Leu Phe Gly

210 215 220

Cys Asp Pro Gly Pro Gly Ala Gln Ile Gly Gly Cys Ile Ala Asn Ser

225 230 235 240

Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu Asn Ile

245 250 255

Val Asn Ile Thr Val Val Leu Ala Asp Gly Thr Val Val Lys Thr Lys

260 265 270

Arg Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asn Leu Asn Gly Leu Phe

275 280 285

Val Gly Ser Glu Gly Thr Leu Gly Val Val Thr Gln Ala Thr Val Lys

290 295 300

Cys His Val Met Pro Lys Ile Glu Thr Val Ala Val Val Ser Phe Pro

305 310 315 320

Thr Val Gly Asp Ala Ala Ser Cys Ser Ser His Ile Ile Gln Glu Gly

325 330 335

Ile Gln Leu Asn Ala Met Glu Leu Leu Asp Asp Asn Met Met Lys Val

340 345 350

Ile Asn Gln Ser Gly Ala Thr Ser Lys Thr Asp Trp Val Glu Ser Pro

355 360 365

Thr Leu Phe Phe Lys Ile Gly Gly Arg Ser Gln Asn Asn Val Lys Glu

370 375 380

Leu Val Asp Glu Val Ser Lys Ile Ala Gln Lys Tyr His Cys Lys Lys

385 390 395 400

Phe Glu Phe Ala Ser Ser Asp Asp Glu Lys Leu Glu Leu Trp Glu Ala

405 410 415

Arg Lys Val Ala Leu Trp Ser Thr Ile Asn Ala Gly Lys Ala Lys Asp

420 425 430

Ser Asn Ser Asn Val Trp Thr Thr Asp Val Ala Val Pro Leu Ser Gln

435 440 445

Phe Ala Pro Val Ile Glu Ala Thr Lys Gln Glu Met Asn Ala Ser Gly

450 455 460

Leu Ile Thr Thr Leu Val Gly His Ala Gly Asp Gly Asn Phe His Ala

465 470 475 480

Phe Ile Val Tyr Asn Asp Asp Gln Arg Asp Ile Ala Glu Lys Ile Val

485 490 495

Asp Asn Met Val His Arg Ala Ile Glu Ala Glu Gly Thr Cys Thr Gly

500 505 510

Glu His Gly Val Gly Ile Gly Lys Arg Lys Phe Leu Leu His Glu Leu

515 520 525

Gly Ala Glu Thr Val Asp Leu Met Arg Gln Leu Lys Leu Ala Leu Asp

530 535 540

Pro Lys Arg Ile Leu Asn Pro Asp Lys Ile Phe Lys Ile Asp Pro Asn

545 550 555 560

Glu His Glu His

<210> 37

<211> 530

<212> PRT

<213> 贝酵母(Saccharomyces bayanus)

<400> 37

Met Leu Arg Asn Ile Leu Val Arg Gly Ala Gly Lys His Leu Lys Tyr

1 5 10 15

Gly Cys Arg Tyr Val Lys Ser Ser Ala Leu Leu Gly Cys Arg Thr Lys

20 25 30

Ile Asn Tyr Tyr Ser Thr Lys Ile Gln Thr Lys Leu Thr Ser Glu Asn

35 40 45

Tyr Pro Asp Val Gln Arg Asp Ala Arg Phe Lys Lys Leu Thr Ser Asp

50 55 60

Asp Leu Asn Tyr Phe Arg Ser Ile Leu Ser Glu Gln Glu Val Leu Gln

65 70 75 80

Ala Ser Glu Leu Glu Asp Leu Ser Phe Tyr Asn Glu Asp Trp Met Arg

85 90 95

Lys Tyr Lys Gly Gln Ser Lys Leu Val Leu Arg Pro Lys Thr Val Glu

100 105 110

Lys Ile Ser Leu Ile Leu Asn Tyr Cys Asn Asp Glu Lys Ile Ala Val

115 120 125

Val Pro Gln Gly Gly Asn Thr Gly Leu Val Gly Gly Ser Val Pro Ile

130 135 140

Phe Asp Glu Leu Ile Leu Ser Leu Ala Asn Leu Asn Lys Ile Arg Asp

145 150 155 160

Phe Asp Pro Val Ser Gly Ile Leu Lys Cys Asp Ala Gly Val Ile Leu

165 170 175

Glu Asn Ala Asn Asn Tyr Val Met Glu Gln Asn Tyr Met Phe Pro Leu

180 185 190

Asp Leu Gly Ala Lys Gly Ser Cys His Val Gly Gly Val Val Ala Thr

195 200 205

Asn Ala Gly Gly Leu Arg Leu Leu Arg Tyr Gly Ser Leu His Gly Ser

210 215 220

Val Leu Gly Leu Glu Val Val Met Pro Asn Gly Gln Ile Val Asn Ser

225 230 235 240

Met His Ser Met Arg Lys Asp Asn Thr Gly Tyr Asp Leu Lys Gln Leu

245 250 255

Phe Ile Gly Ser Glu Gly Thr Ile Gly Ile Ile Thr Gly Val Ser Val

260 265 270

Leu Thr Val Pro Lys Pro Lys Ala Phe Asn Val Ser Tyr Leu Ala Val

275 280 285

Glu Ser Phe Glu Asp Val Gln Lys Val Phe Val Arg Ala Arg Gln Glu

290 295 300

Leu Ser Glu Ile Leu Ser Ala Phe Glu Phe Met Asp Ser Lys Ser Gln

305 310 315 320

Ile Leu Ala Lys Ser Gln Leu Lys Asp Ala Thr Phe Pro Leu Glu Asp

325 330 335

Glu His Pro Phe Tyr Ile Leu Ile Glu Thr Ser Gly Ser Asn Lys Asp

340 345 350

His Asp Asp Ser Lys Leu Glu Thr Phe Leu Glu Ser Val Met Glu Glu

355 360 365

Gly Val Val Thr Asp Gly Val Val Ala Gln Asp Glu Thr Glu Leu Gln

370 375 380

Asn Leu Trp Lys Trp Arg Glu Met Ile Pro Glu Ala Ser Gln Ala Asn

385 390 395 400

Gly Gly Val Tyr Lys Tyr Asp Val Ser Leu Pro Leu Lys Asp Leu Tyr

405 410 415

Ser Leu Val Asp Ala Thr Asn Ala Arg Leu Ser Glu Ala Gly Leu Val

420 425 430

Gly Asp Ser Pro Lys Pro Val Val Gly Ala Ile Gly Tyr Gly His Val

435 440 445

Gly Asp Gly Asn Leu His Leu Asn Val Ala Val Arg Glu Tyr Asn Lys

450 455 460

Glu Ile Glu Lys Val Leu Glu Pro Phe Val Tyr Glu Phe Val Ser Ser

465 470 475 480

Lys His Gly Ser Val Ser Ala Glu His Gly Leu Gly Phe Gln Lys Lys

485 490 495

Asn Tyr Ile Gly Tyr Ser Lys Ser Pro Glu Glu Val Thr Met Ile Lys

500 505 510

Asp Leu Lys Ala His Tyr Asp Pro Asn Ala Ile Leu Asn Pro Tyr Lys

515 520 525

Tyr Val

530

<210> 38

<211> 597

<212> PRT

<213> 鲁氏接合酵母(Zygosaccharomyces rouxii)

<400> 38

Met Val Gly Ser Ile Val Leu Leu Gln Pro Trp Lys Arg Ala Val Ser

1 5 10 15

His Ala Val Arg Pro Arg Thr Tyr Gln Tyr Arg Tyr Phe Arg Thr Thr

20 25 30

Ala Arg Ile Ser Ala Asn Asp Lys Pro Gln Ser Phe Lys Asn Gln Leu

35 40 45

Tyr Glu Ser Thr Gln Gln Arg Leu Arg Arg Glu Arg Ala Glu Gln Glu

50 55 60

Arg Phe Ser Gln Tyr Gln Thr Gln Ser Pro Gly Gly Arg Tyr Ala Ala

65 70 75 80

Leu Thr Phe Ala Leu Val Phe Phe Ser Thr Gly Ala Tyr Tyr Leu Gly

85 90 95

Ser Leu Lys Pro Ala Ser Leu Pro Thr Ser Ser Thr Thr Thr Leu Phe

100 105 110

Glu Ile Glu Pro Pro Leu His Asn Ile Ser Pro Ala Asn Leu Gln Ala

115 120 125

Ala Trp Ala Asp Phe Val Glu Ile Leu Gly Lys Glu Asn Val Ser Thr

130 135 140

Glu His Gly Asp Leu Asp Val His Ser Gly Ser Asp Trp Ser Ser Tyr

145 150 155 160

Thr Leu Lys Lys Asp Glu Arg Pro Phe Leu Val Leu Tyr Pro Ser Thr

165 170 175

Thr Glu Glu Val Ser Arg Ile Met Lys Val Cys His Gln Arg Val Ile

180 185 190

Pro Val Thr Pro Tyr Ser Gly Gly Thr Ser Leu Glu Gly His Phe Ala

195 200 205

Pro Thr Arg Gly Gly Val Cys Ile Asp Phe Arg Arg Met Asn Arg Ile

210 215 220

Leu Glu Leu His Lys Lys Asp Leu Asp Val Val Val Gln Pro Ala Val

225 230 235 240

Gly Trp Glu Asp Leu Asn Glu Glu Leu Ser Lys Asp Gly Leu Phe Phe

245 250 255

Pro Pro Asp Pro Gly Pro Gly Ala Met Ile Gly Gly Met Val Gly Thr

260 265 270

Gly Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Arg Glu Trp

275 280 285

Val Leu Ser Leu Thr Val Val Leu Ala Asp Gly Thr Val Ile Lys Thr

290 295 300

Arg Gln Arg Pro Arg Lys Ser Ser Ala Gly Tyr Asp Leu Thr Arg Leu

305 310 315 320

Phe Ile Gly Ser Glu Gly Thr Leu Gly Leu Val Thr Glu Ala Thr Leu

325 330 335

Lys Leu Thr Val Lys Pro Lys Ser Gln Ser Val Ala Val Ala Ser Phe

340 345 350

Pro Ser Ile His Asn Ala Ala Glu Cys Val Thr Arg Val Val Glu Glu

355 360 365

Gly Ile Pro Val Ala Gly Val Glu Ile Leu Asp Asp Val Gln Met Lys

370 375 380

Cys Ile Asn Asp Ser Arg Thr Thr Arg Arg Gln Trp Lys Glu Ser Pro

385 390 395 400

Thr Leu Phe Phe Lys Phe Thr Gly Thr Pro Val Gly Val Lys Glu Gln

405 410 415

Ile Glu Leu Val Arg Lys Ile Val Ser Ser Ser Ala Gly Gln Ser Phe

420 425 430

Glu Phe Ala Arg Gly Glu Asp Glu Met Lys Glu Leu Trp Ser Ala Arg

435 440 445

Lys Glu Ala Leu Trp Ser Val Met Ser Met Arg Arg Gly Pro Glu Asp

450 455 460

Arg Val Trp Thr Thr Asp Val Ala Val Pro Met Ser Lys Leu Pro Asp

465 470 475 480

Ile Ile Glu Ala Thr Lys Gln Asp Met Thr Glu Ser Gly Leu Leu Ala

485 490 495

Gly Ile Cys Gly His Val Gly Asp Gly Asn Phe His Ala Ile Ile Leu

500 505 510

Phe Asn Glu Asp Glu Lys Lys Ile Ala Glu Gly Val Val His Arg Met

515 520 525

Val Lys Arg Ala Val Glu Met Glu Gly Thr Val Thr Gly Glu His Gly

530 535 540

Val Gly Leu Ile Lys Arg Asp Tyr Leu Gln His Glu Val Gly Glu Thr

545 550 555 560

Thr Val Asp Thr Met Arg Arg Ile Lys Met Ala Leu Asp Pro Leu Arg

565 570 575

Leu Leu Asn Cys Asp Lys Val Val Arg Val Glu Gln Pro Thr Thr Val

580 585 590

Glu Leu Lys Lys Trp

595

<210> 39

<211> 576

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 39

Met Phe Arg Phe Val Gly Arg Ser Gly Phe Ala Leu Arg Gly Ser Leu

1 5 10 15

Gln Leu Arg Lys Asp Val Leu Arg Ser Arg Thr Thr Ala Val Ala Lys

20 25 30

Arg His Tyr Ser Ser Ser Asn Gly Asn Asn Gly Gly Gly Phe Ser Ser

35 40 45

Ala Ile Leu Ser Val Leu Gly Gly Ser Leu Ile Gly Gly Gly Phe Val

50 55 60

Ala Tyr Ala Leu Gly Ser Gln Phe Glu Lys Glu Lys Ser Val Ser Asp

65 70 75 80

Leu Ser Ile Ala Arg Leu Glu Asp Leu Asp Ser Pro Glu Tyr Cys Asp

85 90 95

Lys Glu Thr Phe Ala Lys Ala Leu Val Glu Leu Lys Asp Val Leu Glu

100 105 110

Asn Asp Pro Glu Asn Phe Thr Val Ala Lys Asp Asp Leu Asp Ala His

115 120 125

Ser Asp Thr Tyr Phe Asn Ser His His Ala Glu Ala Asn Gln Arg Pro

130 135 140

Glu Ile Val Leu Tyr Pro Arg Asn Thr Glu Asp Val Ser Lys Leu Leu

145 150 155 160

Lys Ile Cys His Lys Tyr Ser Ile Pro Val Ile Pro Phe Ser Gly Gly

165 170 175

Thr Ser Leu Glu Gly His Phe Leu Pro Thr Arg Pro Gly Ser Cys Val

180 185 190

Val Leu Asp Ile Ser Lys Tyr Leu Asn Lys Ile Ile Gln Leu Asn Lys

195 200 205

Glu Asp Leu Asp Val Val Val Gln Gly Gly Val Pro Trp Glu Glu Leu

210 215 220

Asn Glu Tyr Leu Asn Asp His Gly Leu Leu Phe Gly Cys Asp Pro Gly

225 230 235 240

Pro Gly Ala Gln Ile Ala Gly Cys Ile Ala Asn Ser Cys Ser Gly Thr

245 250 255

Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu Asn Val Val Asn Ile Thr

260 265 270

Met Cys Met Ala Asp Gly Thr Ile Val Lys Thr Lys Arg Arg Pro Arg

275 280 285

Lys Ser Ser Ala Gly Tyr Asn Leu Asn Gly Leu Ile Ile Gly Ser Glu

290 295 300

Gly Thr Leu Gly Ile Val Thr Glu Ala Thr Ile Lys Cys His Val Arg

305 310 315 320

Ser Thr Phe Glu Thr Val Ala Val Val Pro Phe Pro Thr Val Ser Asp

325 330 335

Ala Ala Ser Cys Ser Ser His Leu Ile Gln Ala Gly Ile Gln Leu Asn

340 345 350

Ala Met Glu Leu Leu Asp Asp Asn Met Met Lys Ile Ile Asn Gln Ser

355 360 365

Gly Ala Thr Ser Lys Asp Asn Trp Val Glu Ser Pro Thr Leu Phe Phe

370 375 380

Lys Ile Gly Gly Arg Ser Glu Gln Ile Ile Gln Glu Val Ile Lys Glu

385 390 395 400

Val Glu Lys Ile Ala Ser Gln His Asn Asn Thr Lys Phe Glu Phe Ala

405 410 415

Thr Asp Glu Asp Ser Lys Leu Glu Leu Trp Glu Ala Arg Lys Val Ala

420 425 430

Leu Trp Ser Thr Ile Asp Thr Gly Arg Lys Thr Asn Pro Asp Ala Asn

435 440 445

Ile Trp Thr Thr Asp Val Ala Val Pro Ile Ser Lys Phe Ala Asp Val

450 455 460

Ile Asn Ala Thr Lys Glu Glu Met Asn Ala Ser Gly Leu Leu Thr Ser

465 470 475 480

Leu Val Gly His Ala Gly Asp Gly Asn Phe His Ala Phe Ile Ile Tyr

485 490 495

Asn Thr Glu Gln Arg Lys Thr Ala Glu Thr Ile Val Glu Asn Met Val

500 505 510

Lys Arg Ala Ile Asp Ala Glu Gly Thr Cys Thr Gly Glu His Gly Val

515 520 525

Gly Ile Gly Lys Arg Asp Tyr Leu Leu Glu Glu Val Gly Glu Asp Thr

530 535 540

Val Ala Val Met Arg Lys Leu Lys Leu Ala Leu Asp Pro Lys Arg Ile

545 550 555 560

Leu Asn Pro Asp Lys Ile Phe Lys Ile Asp Pro Asn Asp His Gln His

565 570 575

<210> 40

<211> 582

<212> PRT

<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)

<400> 40

Met Phe Lys Phe Val Gly Arg Ser Gly Thr Ala Leu Arg Ser Ser Leu

1 5 10 15

Gln Ala Arg Arg Asn Gly Ala Val Trp Lys Arg Gly Tyr Ala Ser Gly

20 25 30

Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Ser Ser Leu Val Leu

35 40 45

Gly Val Leu Ala Gly Ser Met Ile Gly Gly Gly Ile Val Ala Tyr Thr

50 55 60

Leu Gly Asn Gln Phe Glu Lys Asp Lys Lys Val Ser Gly Ser Ser Ala

65 70 75 80

Ala Ala Ala Ala Ser Asp Leu Ser Ile Leu Lys Leu Glu Asp Leu Glu

85 90 95

Ser Pro Val Tyr Cys Asp Ser Glu Thr Phe Ser Thr Ala Val Glu Glu

100 105 110

Leu Lys Thr Val Leu Asn Asn Asp Pro Glu Asn Phe Thr Thr Ala Lys

115 120 125

Ala Asp Leu Asp Ser His Ser Asp Thr Tyr Phe Asn Ser His His Ala

130 135 140

Glu Pro Asp Gln Arg Pro Glu Ile Val Leu Phe Pro Arg Asn Thr Glu

145 150 155 160

Asp Val Ser Gln Leu Leu Lys Ile Cys His Lys Tyr Ser Ile Pro Val

165 170 175

Ile Pro Phe Ser Gly Gly Thr Ser Leu Glu Gly His Phe Leu Pro Thr

180 185 190

Arg Pro Gly Ser Cys Val Val Leu Asp Ile Ser Lys Tyr Leu Asn Lys

195 200 205

Ile Ile His Leu Asn Lys Glu Asp Leu Asp Val Val Val Gln Gly Gly

210 215 220

Val Pro Trp Glu Asp Leu Asn Asp Tyr Leu Ile Asp Gln Gly Leu Leu

225 230 235 240

Phe Gly Cys Asp Pro Gly Pro Gly Ala Gln Ile Ala Gly Cys Ile Ala

245 250 255

Asn Ser Cys Ser Gly Thr Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu

260 265 270

Asn Val Val Asn Ile Thr Met Cys Met Ala Asp Gly Thr Ile Ile Lys

275 280 285

Thr Lys Lys Arg Pro Arg Lys Ser Ala Ala Gly Tyr Asn Leu Asn Gly

290 295 300

Leu Ile Val Gly Ser Glu Gly Thr Leu Gly Ile Val Thr Glu Ala Thr

305 310 315 320

Ile Lys Cys His Val Arg Ser Leu Phe Glu Thr Val Ala Val Val Pro

325 330 335

Phe Pro Thr Val Ser Asp Ala Ala Ser Cys Ser Ser His Leu Ile Gln

340 345 350

Ala Gly Ile Gln Leu Asn Ala Met Glu Leu Leu Asp Asp Asn Met Met

355 360 365

Lys Ile Ile Asn Gln Ser Gly Ala Thr Ser Lys Ile Asp Trp Val Glu

370 375 380

Ser Pro Thr Leu Phe Phe Lys Ile Gly Gly Arg Thr Glu Lys Ser Ile

385 390 395 400

Gln Asp Val Val Asp Glu Val Glu Lys Ile Ala Ser Gln His Asn Asn

405 410 415

Tyr Lys Phe Glu Phe Ala Thr Asp Ala Asp Ser Lys Leu Glu Leu Trp

420 425 430

Glu Ala Arg Lys Val Ala Leu Trp Ser Thr Ile Asp Ala Gly Lys Lys

435 440 445

Leu Asp Pro Lys Val Asn Val Trp Thr Thr Asp Val Ala Val Pro Ile

450 455 460

Ser Arg Phe Ala Glu Val Ile Asn Ala Thr Lys Gln Glu Met Asn Ser

465 470 475 480

Ser Gly Leu Leu Thr Ser Leu Val Gly His Ala Gly Asp Gly Asn Phe

485 490 495

His Ala Phe Ile Ile Tyr Asn Ser Glu Gln Arg Lys Gln Ala Glu Thr

500 505 510

Ile Val Glu Asn Met Val Lys Arg Ala Ile Asp Ala Glu Gly Thr Cys

515 520 525

Thr Gly Glu His Gly Val Gly Ile Gly Lys Arg Asp Tyr Leu Val Glu

530 535 540

Glu Leu Gly Glu Asp Ala Ile Ala Val Met Arg Lys Leu Lys Leu Ala

545 550 555 560

Leu Asp Pro Lys Arg Ile Leu Asn Pro Asp Lys Ile Phe Lys Ile Asp

565 570 575

Pro Asn Asp Asn Gln His

580

<210> 41

<211> 592

<212> PRT

<213> 马克斯克鲁维酵母(Kluyveromyces marxianus)

<400> 41

Met Leu Pro Arg Phe Val Val Arg Ser Gly Ala Ala Gly Arg Asn Leu

1 5 10 15

Gly Phe Ser Phe Thr Arg Lys Cys Asp His Thr Phe Leu Ser Lys Thr

20 25 30

Val Arg Asn Asp Leu Ser His Arg Pro Tyr Ser Thr Gly Thr Asn Gly

35 40 45

Asn Gly Ser Ala Asn Ala Gly Lys Ser Gln Ser Gly Leu Leu Phe Gly

50 55 60

Val Phe Gly Gly Thr Leu Ile Gly Gly Gly Leu Val Ala Tyr Phe Leu

65 70 75 80

Gly Ser Lys Phe Asp Gln Gln Gln Ser Ser Ser Gln Gln Val Ser Asp

85 90 95

Leu Ser Ile Ala Arg Leu Glu Asp Leu Asp Ser Pro Lys Tyr Cys Asp

100 105 110

Lys Lys Thr Phe Ala Thr Ala Val Glu Glu Leu Lys Gln Val Leu Asp

115 120 125

Asn Asn Pro Glu Asn Phe Ser Asp Ala Lys Ser Asp Leu Asp Ser His

130 135 140

Ser Asp Thr Tyr Phe Asn Ser His His Ala Thr Pro Glu Gln Arg Pro

145 150 155 160

Glu Ile Val Leu Phe Pro Arg Asn Thr Glu Asp Val Ser Lys Leu Leu

165 170 175

Lys Ile Cys His Lys Tyr Ser Ile Pro Val Ile Pro Phe Ser Gly Gly

180 185 190

Thr Ser Leu Glu Gly His Phe Met Pro Thr Arg Pro Gly Ser Cys Val

195 200 205

Val Leu Asp Ile Ser Lys Tyr Met Asn Gln Ile Ile Gln Leu Asn Lys

210 215 220

Glu Asp Leu Asp Val Val Val Gln Gly Gly Val Pro Trp Glu Asp Leu

225 230 235 240

Asn Asp Tyr Leu Asn Asp His Gly Leu Leu Phe Gly Cys Asp Pro Gly

245 250 255

Pro Gly Ala Gln Ile Ala Gly Cys Ile Ala Asn Ser Cys Ser Gly Thr

260 265 270

Asn Ala Tyr Arg Tyr Gly Thr Met Lys Glu Asn Val Val Asn Ile Thr

275 280 285

Met Cys Leu Ala Asp Gly Thr Ile Ile Lys Thr Lys Arg Arg Pro Arg

290 295 300

Lys Ser Ser Ala Gly Tyr Asn Leu Asn Gly Leu Ile Ile Gly Ser Glu

305 310 315 320

Gly Thr Leu Gly Ile Val Thr Glu Ala Thr Ile Lys Cys His Val Arg

325 330 335

Ser Asn Phe Glu Thr Val Ala Val Val Pro Phe Pro Ser Val Ala Asp

340 345 350

Ala Ala Ser Cys Ser Ser His Leu Ile Gln Ala Gly Ile Gln Leu Asn

355 360 365

Ala Met Glu Leu Leu Asp Asp Asn Met Met Lys Ile Ile Asn Lys Ser

370 375 380

Gly Ala Thr Ser Arg Thr Asn Trp Val Glu Ser Pro Thr Leu Phe Phe

385 390 395 400

Lys Ile Gly Gly Arg Ser Glu Lys Ala Ile Lys Glu Val Val Lys Glu

405 410 415

Val Glu Lys Ile Ala Ser Gln His Asn Asn Ser Asn Phe Glu Phe Ala

420 425 430

Ser Asp Glu Glu Thr Lys Leu Glu Leu Trp Glu Ala Arg Lys Val Ala

435 440 445

Leu Trp Ser Thr Ile Asp Ala Gly Lys Lys Leu Asp Pro Asn Val Asn

450 455 460

Val Trp Thr Thr Asp Val Ala Val Pro Ile Ser Lys Phe Ala Gln Val

465 470 475 480

Ile Asn Asp Thr Lys Glu Glu Met Asn Ala Ser Gly Leu Leu Thr Ser

485 490 495

Leu Val Gly His Ala Gly Asp Gly Asn Phe His Ala Phe Ile Ile Tyr

500 505 510

Asn Ala Glu Gln Arg Lys Thr Ala Glu Thr Ile Val Glu Asn Met Val

515 520 525

Lys Arg Ala Ile Asp Ala Glu Gly Thr Cys Thr Gly Glu His Gly Val

530 535 540

Gly Ile Gly Lys Arg Glu Phe Leu Val Glu Glu Leu Gly Glu Asp Thr

545 550 555 560

Ile Ala Val Met Arg Lys Leu Lys Leu Ala Leu Asp Pro Lys Arg Ile

565 570 575

Leu Asn Pro Asp Lys Val Phe Lys Ile Asp Pro Asn Asp His Gln His

580 585 590

<210> 42

<211> 1211

<212> PRT

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 42

Met Lys Arg Ile Arg Ser Leu Trp Phe Asn Ala Glu Ala Ser Tyr Ser

1 5 10 15

Asn Leu Asn Asn Ser Pro Ser Leu Arg Asn Lys Asn Ser Thr Gly Asn

20 25 30

Asn Ser Arg Ser Lys Asn Tyr Arg Ser Phe Ser Arg Phe Asp Leu Ile

35 40 45

Asn Ser Ile Leu Leu Leu Met Met Leu Phe Leu Leu Ala Ile Phe Val

50 55 60

Thr Ala Leu Tyr Leu Thr Lys Ser Ser Arg Leu Thr Tyr Ser His Ala

65 70 75 80

Ser Arg Ala Ala Leu Phe Asn Pro Leu Gly Val Ile Ser Pro Ser Leu

85 90 95

Gly Asn His Thr Leu Asn Tyr Asp Pro Glu Ala Arg Glu Ser Ser Lys

100 105 110

Lys Leu Tyr Glu Leu Leu Ser Asp Phe Asn Thr Ala Tyr Tyr Asp Asp

115 120 125

Glu Asn Met Ile Leu Gly Ser Asn Leu Phe Ser Lys Asn Thr Tyr Ser

130 135 140

Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn

145 150 155 160

Ile Gly Phe Gly Tyr Ala Leu Asp Thr Leu Asn Phe Tyr Thr Asp Ala

165 170 175

Pro Gly Ala Leu Asn Asn Gly Trp Pro Leu Arg Asn His Arg Phe Ala

180 185 190

Gly Ala Phe Val Ser Asp Phe Tyr Cys Leu Gln Pro Lys Leu Asn Ser

195 200 205

Thr Asn Phe Pro Glu Leu Asp Asp Val Gly Tyr Ser Thr Val Ile Ser

210 215 220

Ser Ile Pro Gln Trp Thr Asn Leu Gln Phe Ser Leu Val Asn Asp Ser

225 230 235 240

Lys Trp Phe Asn Pro Gln Asn Val Thr Leu Asp Asp Val Thr Asn Tyr

245 250 255

Ser Gln Asn Leu Ser Met Lys Asp Gly Ile Val Thr Thr Glu Leu Asp

260 265 270

Trp Leu Asn Ser Gln Ile His Val Lys Ser Glu Ile Trp Ala His Arg

275 280 285

His Ile His Pro Leu Gly Val Val Ser Leu Glu Ile Ser Leu Asn Thr

290 295 300

Asp His Leu Pro Ser Asp Phe Asp Ser Leu Asp Val Asn Ile Trp Asp

305 310 315 320

Ile Leu Asp Phe Asn Thr Ser His Arg Thr Val Leu His Ser Thr Gly

325 330 335

Thr Asp Glu Lys Asn Asn Ala Val Phe Met Ile Val Gln Pro Asp Asn

340 345 350

Val Pro Ser Ser Asn Cys Ala Ile Tyr Ser Thr Cys Thr Val Lys Tyr

355 360 365

Glu Asn Ser Thr Asn Pro Ile Asn Ser Ser Glu Ser Phe Glu Glu Lys

370 375 380

Asp Val Ser Ser Asn Ile Tyr Asn Val Ile Leu Thr Glu Asp Gln Pro

385 390 395 400

Lys Ile Ile Val His Lys Tyr Val Gly Ile Met Ser Thr Glu Phe Asn

405 410 415

Lys Asn Lys Glu Gln Gln Asp Asn Thr Asn Ile Gly Leu Ala Lys Met

420 425 430

Ile Ala Leu Asn Ser Lys Gly Asn Tyr Glu Lys Leu Leu Ser Ser His

435 440 445

Lys Arg Ala Trp Tyr Asp Leu Tyr Asn Asp Ala Phe Ile Glu Ile Pro

450 455 460

Ser Asp Ser Leu Leu Glu Met Thr Ala Arg Ser Ser Leu Phe His Leu

465 470 475 480

Leu Ala Asn Thr Arg Asp Tyr Asn Val Ser Ser Asp Arg Gly Leu Pro

485 490 495

Val Gly Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe

500 505 510

Trp Asp Ala Asp Ile Trp Met Glu Pro Ala Leu Leu Pro Phe Phe Pro

515 520 525

Asn Val Ala Gln Asn Met Asn Asn Tyr Arg Asn Ala Thr His Ser Gln

530 535 540

Ala Lys Leu Asn Ala Glu Lys Tyr Gly Tyr Pro Gly Ala Ile Tyr Pro

545 550 555 560

Trp Thr Ser Gly Lys Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Val

565 570 575

Asp Tyr Glu Tyr His Ile Asn Val Asp Val Ala Met Ala Ser Phe Ser

580 585 590

Ile Tyr Leu Asn Gly His Glu Gly Ile Asp Asp Glu Tyr Leu Arg Tyr

595 600 605

Thr Thr Trp Pro Ile Ile Lys Asn Ala Ala Gln Phe Phe Thr Ala Tyr

610 615 620

Val Lys Tyr Asn Ser Ser Leu Gly Leu Tyr Glu Thr Tyr Asn Leu Thr

625 630 635 640

Asp Pro Asp Glu Phe Ala Asn His Ile Asn Asn Gly Ala Phe Thr Asn

645 650 655

Ala Gly Ile Lys Thr Leu Leu Lys Trp Ala Thr Asp Ile Gly Asn His

660 665 670

Leu Gly Glu Val Val Asp Pro Lys Trp Ser Glu Ile Ser Lys Asp Ile

675 680 685

Tyr Ile Pro Arg Ser Ser Ser Asn Ile Thr Leu Glu Tyr Ser Gly Met

690 695 700

Asn Ser Ser Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val Tyr

705 710 715 720

Pro Leu Gly Tyr Ile Asn Asp Glu Ser Ile Leu Asn Asn Ala Ile Lys

725 730 735

Asp Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met

740 745 750

Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Gly Leu Leu Asn His Gly

755 760 765

Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Leu Pro Tyr Leu Arg

770 775 780

Ala Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr

785 790 795 800

Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly

805 810 815

Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile Arg Tyr Ser Tyr

820 825 830

Glu Val Asp Pro Asp Thr Lys Lys Ile Asn Arg Leu Leu Arg Phe Asn

835 840 845

Pro Ile Glu Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn Phe

850 855 860

Lys Tyr Met Asn Gln Val Leu Asp Ile Ile Ile Asp Asp His Asn Gly

865 870 875 880

Thr Ile Val His Lys Ser Gly Asp Val Pro Ile His Ile Lys Ile Pro

885 890 895

Asn Arg Ser Leu Ile His Asp Gln Asp Ile Asn Phe Tyr Asn Gly Ser

900 905 910

Glu Asn Glu Arg Lys Pro Asn Leu Glu Arg Arg Asp Val Asp Arg Val

915 920 925

Gly Asp Pro Met Arg Met Asp Arg Tyr Gly Thr Tyr Tyr Leu Leu Lys

930 935 940

Pro Lys Gln Glu Leu Thr Val Gln Leu Phe Lys Pro Gly Leu Asn Ala

945 950 955 960

Arg Asn Asn Ile Ala Glu Asn Lys Gln Ile Thr Asn Leu Thr Ala Gly

965 970 975

Val Pro Gly Asp Val Ala Phe Ser Ala Leu Asp Gly Asn Asn Tyr Thr

980 985 990

His Trp Gln Pro Leu Asp Lys Ile His Arg Ala Lys Leu Leu Ile Asp

995 1000 1005

Leu Gly Glu Tyr Asn Glu Lys Glu Ile Thr Lys Gly Met Ile Leu

1010 1015 1020

Trp Gly Gln Arg Pro Ala Lys Asn Ile Ser Ile Ser Ile Leu Pro

1025 1030 1035

His Ser Glu Lys Val Glu Asn Leu Phe Ala Asn Val Thr Glu Ile

1040 1045 1050

Met Gln Asn Ser Gly Asn Asp Gln Leu Leu Asn Glu Thr Ile Gly

1055 1060 1065

Gln Leu Leu Asp Asn Ala Gly Ile Pro Val Glu Asn Val Ile Asp

1070 1075 1080

Phe Asp Gly Ile Glu Gln Glu Asp Asp Glu Ser Leu Asp Asp Val

1085 1090 1095

Gln Ala Leu Leu His Trp Lys Lys Glu Asp Leu Ala Lys Leu Ile

1100 1105 1110

Glu Gln Ile Pro Arg Leu Asn Phe Leu Lys Arg Lys Phe Val Lys

1115 1120 1125

Ile Leu Asp Asn Val Pro Val Ser Pro Ser Glu Pro Tyr Tyr Glu

1130 1135 1140

Ala Ser Arg Asn Gln Ser Leu Ile Glu Ile Leu Pro Ser Asn Arg

1145 1150 1155

Thr Thr Phe Thr Ile Asp Tyr Asp Lys Leu Gln Val Gly Asp Lys

1160 1165 1170

Gly Asn Thr Asp Trp Arg Lys Thr Arg Tyr Ile Val Val Ala Val

1175 1180 1185

Gln Gly Val Tyr Asp Asp Tyr Asp Asp Asp Asn Lys Gly Ala Thr

1190 1195 1200

Ile Lys Glu Ile Val Leu Asn Asp

1205 1210

<210> 43

<211> 1186

<212> PRT

<213> 德尔布有孢圆酵母(Torulaspora delbrueckii)

<400> 43

Met Ser Gly Arg Gln Arg Leu Leu Glu Ser Ser Val Ala Thr Asp Pro

1 5 10 15

Ser Asn Thr Arg Arg Ser Lys Thr Ser Ala Leu Trp Phe Trp Val Leu

20 25 30

Ile Ala Ser Met Val Ala Leu Thr Thr Val Asn Phe Phe Leu Ala Asn

35 40 45

Ile Ala Ser Phe Val Ser Gly Leu Ala Asn Leu His Glu Pro Gln Val

50 55 60

Gly Ala Ile Lys Pro Thr Val Thr His His Thr Leu Asp Cys Asn Glu

65 70 75 80

Asn Ala Arg Lys Ser Ser Lys Lys Leu Tyr Glu Leu Leu Tyr Asp Phe

85 90 95

Glu Ala Ser Leu Tyr Asp Asp Glu Asn Met Ile Leu Gly Ser Thr Lys

100 105 110

Phe Tyr Pro Asn Thr Tyr Ser Lys Gln Pro Tyr Val Ser Asn Gly Tyr

115 120 125

Ile Gly Ser Arg Ile Pro Asn Ile Gly Phe Gly Tyr Ala Glu Asp Thr

130 135 140

Leu Asn Leu Trp Thr Gly Ser Gly Asp Glu Ile Pro Gly Ala Leu Asn

145 150 155 160

Asn Gly Trp Pro Leu Arg Asn Arg Arg Tyr Ala Gly Ala Phe Val Ser

165 170 175

Asp Phe Tyr Cys Leu Gln Glu Ser Leu Asn Ser Thr Asn Phe Pro Glu

180 185 190

Leu Asp Glu Lys Gly Tyr Thr Thr Val Ile Ser Ser Ile Pro Gln Trp

195 200 205

Thr Asn Leu Gln Phe Thr Ile Asp Asp Asp Ser Val Trp Phe Asn Pro

210 215 220

Gln Asn Val Ser Leu Asp Glu Val Ser Asn Tyr Asn Gln Asn Met Ser

225 230 235 240

Met Arg Asn Gly Val Val Thr Thr Gln Leu Asp Trp Leu Asp Gly Met

245 250 255

Ile Asn Val Arg Ser Glu Ile Val Thr His Lys Lys His Tyr Ala Leu

260 265 270

Gly Met Met Ser Leu Glu Ile Ser Leu Asn Leu Asp Arg Leu Pro Pro

275 280 285

Glu Val Pro Tyr Met Asp Val Asn Val Tyr Asp Val Leu Asp Phe Ser

290 295 300

Thr Ser His Arg Thr Val Leu Gln Ser Leu Gly Phe Asp Lys Glu Asn

305 310 315 320

Gln Gly Ile Phe Met Ile Val Glu Pro Glu Asn Val Pro Tyr Ser Asn

325 330 335

Ala Ala Val Phe Ser Ser Ser Thr Ala Asp Asp Ser Ala Asn Phe Thr

340 345 350

Trp Ser Glu Glu Tyr Ser Thr Asp Glu Lys Ile Val Gln Ser Thr His

355 360 365

Ala Asn Leu Thr Arg Glu Asn Pro Lys Ile Ala Val Lys Lys Tyr Val

370 375 380

Gly Ile Met Ser Thr Glu Phe Asn Thr Asn Ser Ala His Asn Val Ser

385 390 395 400

Asn Ile Glu Phe Ala Lys Ser Val Ser Ser Arg Ser Arg Gly Arg Tyr

405 410 415

Asp Glu Leu Met Ser Thr Asn Thr Lys Ala Trp Ala Ala Ile Tyr Asn

420 425 430

Asp Ala Phe Ile Glu Ile Pro Ser Asp Ala Leu Leu Glu Met Thr Ala

435 440 445

Arg Ser Ser Leu Tyr His Leu Leu Ala Asn Thr Arg Thr Phe Asn Val

450 455 460

Ser Ala Asp Arg Gly Leu Pro Val Pro Val Ser Gly Leu Ser Ser Asp

465 470 475 480

Ser Tyr Gly Gly Met Val Phe Trp Asp Ala Asp Ile Trp Met Gln Pro

485 490 495

Ala Leu Leu Pro Phe Phe Pro Asp Ile Ala Lys Asn Met Asn Asn Tyr

500 505 510

Arg Asn Ala Ser His Gln Gln Ala Arg Leu Asn Ala Gln Glu Asn Gly

515 520 525

Tyr Pro Gly Ala Val Tyr Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys

530 535 540

Thr Ser Thr Gly Pro Cys Ile Asp Tyr Glu Tyr His Ile Asn Val Asp

545 550 555 560

Val Ala Leu Ala Ser Phe Ser Ile Tyr Met Asn Gly Gly Lys Gly Val

565 570 575

Asp Asp Asp Tyr Leu Arg Tyr Thr Thr Trp Pro Met Val Arg Asp Ala

580 585 590

Ala Glu Phe Phe Thr Val Tyr Val Lys Phe Asn Glu Thr Leu Gly Lys

595 600 605

Tyr Glu Thr Tyr Asn Leu Thr Asp Pro Asp Glu Phe Ala Asn His Val

610 615 620

Asn Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp

625 630 635 640

Ala Thr Asp Ile Gly Tyr His Leu Gly Glu Tyr Val Asp Pro Lys Trp

645 650 655

Ile Asp Ile Ala Lys Lys Ile Tyr Ile Pro Arg Ser Cys Ser Asn Ile

660 665 670

Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Glu Ile Lys Gln Ala

675 680 685

Asp Val Pro Leu Met Val Tyr Pro Leu Gly Tyr Ile Thr Asp Glu Ser

690 695 700

Ile Leu Asn Gly Ala Ile Lys Asp Leu Tyr Tyr Tyr Ser Glu Arg Gln

705 710 715 720

Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala

725 730 735

Ala Gly Leu Leu Asn His Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys

740 745 750

Ser Val Leu Pro Tyr Leu Arg Ala Pro Phe Ala Gln Phe Ser Glu Gln

755 760 765

Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro

770 775 780

Phe Leu Thr Ala Asn Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu

785 790 795 800

Thr Gly Leu Arg Tyr Ser Tyr Glu Val Asp Ser Ser Thr Lys Lys Ile

805 810 815

Ser Arg Leu Leu Lys Phe Asn Pro Val Glu Leu Pro Leu Leu Pro Gly

820 825 830

Gly Ile Ala Ile Arg Asn Phe Lys Tyr Met Asn Gln Val Leu Asp Val

835 840 845

Ile Ile Asp Asp Asn Asn Gly Thr Ile Val His Lys Ser Gly Asp Gln

850 855 860

Pro Ile Arg Val Lys Val Pro Asp Arg Ser Tyr Ile Arg Asp Gln Asp

865 870 875 880

Ile Ser Phe Tyr Leu Gly Gly Asn Ser Ser Gly His Asp Asp Cys Phe

885 890 895

Asn Asn Phe Ile Glu Gly Gly Glu Gly Asp Arg Pro Val Ser Ala Phe

900 905 910

Gly Thr Tyr Tyr Thr Ile Asn Pro Gly Glu Glu Leu Val Leu Pro Leu

915 920 925

Phe Lys Pro Gly Leu Asn Val Glu Gly Asn Ile Ala Glu Ser Lys Gln

930 935 940

Val Thr Asn Cys Thr Ala Gly Val Pro Gly Asp Val Pro Phe Ser Ala

945 950 955 960

Leu Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Ala Tyr Lys Tyr Asp

965 970 975

Glu Gly Lys Leu Leu Ile Asp Leu Gly Glu Asp Asn Leu Gln Glu Ile

980 985 990

Lys Ser Gly Met Ile Leu Trp Gly Gln Arg Pro Ala Arg Asn Met Ser

995 1000 1005

Ile Ser Ile Leu Pro His Thr Tyr Glu Val Glu Lys Phe Val Ser

1010 1015 1020

Asn Leu Thr Gln Val Leu Glu His Ser Asp Leu Gln Lys Ile Glu

1025 1030 1035

Gly Glu Asp Ala Ser Glu Val Leu Glu Asp Val Gly Ile Asp Thr

1040 1045 1050

Asn Glu Leu Arg Asn Phe Glu Cys Asp Asp Asp Ile Arg Thr Met

1055 1060 1065

Leu Asn Trp Lys Thr Glu Thr Leu Glu Ser Leu Val Asp His Leu

1070 1075 1080

Lys Lys Ser Lys Met Ile Ser Arg His Phe Val Lys Ile Leu Asp

1085 1090 1095

Asn Tyr Ser Ile Glu Pro Ser Glu Pro Tyr Phe Lys Glu Val Tyr

1100 1105 1110

Asn Lys Ser Leu Ile Glu Ile Leu Pro Ser Asn Lys Thr Glu Phe

1115 1120 1125

Ser Ile Asp Tyr Asp Ala Leu Lys Leu Asn Asp Thr Ala Cys Tyr

1130 1135 1140

Ser Asn Asp Ser Ile Gln Asn Ser Thr Ala Trp Arg Lys Thr Arg

1145 1150 1155

Phe Ile Leu Val Ser Ile Gln Gly Ser Tyr Asp Asp Asp Ile Asp

1160 1165 1170

Ile Lys Gly Gly Thr Ile Lys Glu Ile Val Leu Lys Val

1175 1180 1185

<210> 44

<211> 1245

<212> PRT

<213> 西氏哈萨克酵母(Kazachstania naganishii)

<400> 44

Met Ala Gln Tyr Lys Trp Asn Pro Phe Ser Trp Lys Tyr Ser Thr Leu

1 5 10 15

Glu Asn Lys Lys Glu Ser Asn Thr Gln Tyr Leu Glu His Ser Ser Gly

20 25 30

Arg Glu Glu Asn Ile Pro Leu Asn Pro Asn Thr Ser Tyr Lys Leu Asn

35 40 45

Lys Leu Arg Ser Ile Ser Ser Gly Glu Thr Leu Asn Ser Asn Asp Arg

50 55 60

Ser Asn Ile Ser Ser Lys Arg Lys Gln Gln Ile Ser Ile Gly Phe Leu

65 70 75 80

Gly Val Met Leu Ala Ala Ile Leu Val Ser Met Val Met Val Leu Val

85 90 95

Thr Thr Thr Asn Met Ile Met Ala Ser Pro Phe Cys Lys Arg Phe His

100 105 110

Gly Val Thr Thr Thr Ala Glu Pro Ser Arg Glu His Ile Asp Phe Arg

115 120 125

Gln Gly Val Ile Gln Pro Ala Gly Glu Thr His Glu Leu Pro Tyr Asn

130 135 140

Ala Glu Ala Arg Arg Ala Ser Lys Lys Leu Tyr Glu Leu Leu Ser Asp

145 150 155 160

Phe Asp Thr Ala Phe Tyr Asp Ser Glu Asn Met Ile Leu Gly Thr Thr

165 170 175

Leu Phe Thr Glu Asn Val Tyr Ser Arg Gln Pro His Val Ala Asn Gly

180 185 190

Tyr Ile Gly Asn Arg Val Pro Asn Ile Gly Phe Gly Phe Ala Tyr Asp

195 200 205

Ala Leu Asn Phe Trp Ser Asn Ala Ser Gly Ala Leu Asp Asn Gly Trp

210 215 220

Pro Leu Arg Asn Gln Arg Phe Ala Gly Ser Phe Leu Ser Asp Phe Tyr

225 230 235 240

Cys Leu Glu Pro Lys Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp Asp

245 250 255

Lys Gly Tyr Pro Thr Val Ile Ser Ser Ile Pro Glu Trp Ser Asn Leu

260 265 270

Gln Phe Ser Leu Asn Gly Ser Trp Phe Asn Thr Ser Ser Val His Asn

275 280 285

Glu Asp Val Leu Asn Tyr Asn Gln Asn Val Ser Met Lys Asp Gly Ile

290 295 300

Val Thr Thr Glu Met Asp Trp Tyr Asn Gly Met Val His Val Arg Ser

305 310 315 320

Glu Ile Trp Ala His Arg Arg Ile His Pro Leu Ala Val Met Ser Leu

325 330 335

Glu Val Thr Leu Asn Glu Glu His Leu Pro Asp Asp Phe Asp Gln Leu

340 345 350

Asp Ile Asp Ile His Asp Ile Leu Asp His Ala Thr Ser Met Arg Thr

355 360 365

Val Leu Gln Asp Leu Gln Val Asp Asp Lys Asn Ser Ala Ile Tyr Met

370 375 380

Ile Val Gln Pro Asp Asn Val Pro Asp Ser Asn Ala Ala Ile Tyr Ser

385 390 395 400

Thr Cys Ile Val Ala Asp Ser His Asn Val Ala Gln Asn Asn Phe Glu

405 410 415

Phe Gly Ile Asn Ser Lys Glu Ala Gly Lys Val Ser Gln Ile Ser Arg

420 425 430

Asn Ile Leu Ser Arg Glu Asn Pro Ser Ile Thr Val Thr Lys His Val

435 440 445

Gly Met Met Ser Thr Glu Phe Lys Thr Glu Glu His Phe Leu His Leu

450 455 460

Ser Asn Leu Asp Val Ala Arg Asn Ile Ala Leu Asn Ala Lys Gly Gln

465 470 475 480

Tyr Glu Ser Leu Leu Ser Ser His Lys Arg Ala Trp Tyr Asp Leu Tyr

485 490 495

Asn Asp Ala Phe Ile Glu Ile Pro Ser Asp Ala Leu Leu Glu Met Thr

500 505 510

Ala Arg Ser Ser Leu Tyr Gln Leu Leu Ala Asn Thr Arg Thr Tyr Asn

515 520 525

Val Ser Glu Arg Gly Leu Pro Val Gly Val Gly Gly Leu Ser Ser Asp

530 535 540

Ser Tyr Gly Gly Met Val Phe Trp Asp Ala Asp Ile Trp Met Ala Pro

545 550 555 560

Ala Leu Leu Pro Phe Phe Pro Glu Val Ser Lys Asn Met Asn Asn Tyr

565 570 575

Arg Asn Ser Thr His Lys Gln Ala Gln Leu Asn Ala Gln Glu Ala Gly

580 585 590

Tyr Pro Gly Ala Val Tyr Pro Trp Thr Ser Gly Arg Phe Ala Asn Cys

595 600 605

Thr Ser Thr Gly Pro Cys Ile Asp Tyr Glu Tyr His Ile Asn Val Asp

610 615 620

Val Ala Leu Ala Ser Phe Ser Ile Tyr Leu Asn Gly Ala Ala Gly Ile

625 630 635 640

Asp Asp Asp Tyr Leu Arg Tyr Thr Thr Trp Pro Met Val Arg Asp Ala

645 650 655

Ala Gln Phe Phe Thr Ser Phe Val Lys Tyr Asn Ala Thr Phe Asp Arg

660 665 670

Tyr Glu Thr His Asn Leu Thr Asp Pro Asp Glu Phe Ala Asp His Ile

675 680 685

Asp Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp

690 695 700

Ala Thr Asp Ile Gly Asn His Leu Glu Glu Lys Val Asp Pro Lys Trp

705 710 715 720

Pro Glu Ile Ser Glu Lys Ile Tyr Ile Pro Arg Ser Pro Ser Asn Ile

725 730 735

Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Glu Ile Lys Gln Ala

740 745 750

Asp Val Thr Leu Met Val Tyr Pro Leu Gly Tyr Ile Asn Asp Glu Ser

755 760 765

Ile Leu Met Asn Ala Ile Lys Asp Leu Tyr Tyr Tyr Ser Glu Arg Gln

770 775 780

Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala

785 790 795 800

Ala Gly Leu Leu Asn His Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys

805 810 815

Ser Val Leu Pro Tyr Leu Arg Gln Pro Phe Ala Gln Phe Ser Glu Gln

820 825 830

Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro

835 840 845

Phe Leu Thr Ala Asn Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu

850 855 860

Thr Gly Ile Arg Tyr Ser Tyr Asp Thr Asp Pro Glu Thr Lys Lys Met

865 870 875 880

Ser Arg Val Met Arg Phe Asn Pro Ile Gln Leu Pro Leu Met Pro Gly

885 890 895

Gly Leu Ala Ile Arg Asn Phe Lys Tyr Leu Asn Gln Val Leu Asp Ile

900 905 910

Ile Ile Asp Asp Asn Asn Gly Thr Ile Val His Lys Ser Gly Asp Gln

915 920 925

Pro Ile Thr Ile Leu Val Pro Asn Arg Asp Leu Ile His Asp Arg Asp

930 935 940

Ile Asn Val Tyr Arg Arg Asn Ser Ser Tyr Ala Lys Arg Asp Leu Phe

945 950 955 960

Ser Arg Glu Glu Met Gln Ile Glu Lys Ser Gln Gln Lys Asn Val Ser

965 970 975

Gly Thr Tyr Tyr Thr Leu His Pro Asn Glu Glu Leu Val Leu Pro Leu

980 985 990

Phe Lys Pro Gly Leu Asn Ile Glu Asn Asn Val Ala Glu Asn Gln Gln

995 1000 1005

Ile Thr Asn Leu Thr Ala Gly Val Pro Gly Asp Val Ala Phe Ser

1010 1015 1020

Val Leu Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Leu Thr Lys

1025 1030 1035

Ala Ser Val Gly Lys Leu Leu Ile Asp Leu Gly Arg Asn Asn Glu

1040 1045 1050

Lys Asp Leu Arg His Gly Thr Ile Leu Trp Gly Gln Arg Pro Ala

1055 1060 1065

Lys Asn Val Ser Ile Ser Ile Leu Pro His Asn Glu Leu Ile Glu

1070 1075 1080

Glu Ile Phe Ser Asn Val Thr Tyr Ile Leu Glu Asn Ser Gln Asp

1085 1090 1095

Lys Asp Pro Lys Lys Thr Ile Glu Glu Leu Val Gly Asn Tyr Val

1100 1105 1110

His Val Asn Pro Asp Asn Lys Asp Cys Val Ser Asp Val Leu Thr

1115 1120 1125

Ile Leu Asn Trp Lys Met Asp Asn Val Asp Glu Val Ile Lys Gln

1130 1135 1140

Met Pro Asp Ile Gly Leu Ile His Glu Thr Phe Val Thr Ile Val

1145 1150 1155

Asp Asn Leu Glu Ile Glu Pro Asn Glu Pro Tyr Tyr Asp Glu Leu

1160 1165 1170

Ile Arg Asn Ser Gln Ile Glu Leu Leu Pro Ser Asn Met Thr Val

1175 1180 1185

Phe Asp Ile Asp Tyr Ser Lys Ala Asp Phe Ser Asn Thr Leu Lys

1190 1195 1200

Cys Phe Asn Ser Gly Asn Glu Gln Asp Met Ile Lys Ala Arg Tyr

1205 1210 1215

Ile Val Val Ser Val Gln Gly Thr Tyr Asp Glu Asp Glu Asp Thr

1220 1225 1230

Lys Gly Ala Thr Ile Lys Glu Val Ala Phe Gly Val

1235 1240 1245

<210> 45

<211> 1386

<212> PRT

<213> 蟑螂四盾酵母(Tetrapisispora blattae)

<400> 45

Met Glu Asn Thr Arg Arg Thr Asp Met Pro Ser Asp Arg Asp Ile Ser

1 5 10 15

Asn Ile Gln Ser Gly Asp Asp Lys Ser Asn Ile Glu Met Leu Asp Leu

20 25 30

His His Cys Asp Asp Leu Ser Ile Thr Asp Asn Ile Gln Lys Asn Asp

35 40 45

Leu His Glu Gln Gly Ala Thr Thr Ser Gln Pro Asn Arg Ser Asp Asp

50 55 60

Ile Glu Ala Asn Glu Tyr Thr Leu Phe Asp Ile Met Asp His Ser Glu

65 70 75 80

Glu Asp Asn Asn Gly Asp Ser Gln Glu Asn Ser Asp Asp Gln Glu Asn

85 90 95

Asp Asp Glu Thr Ala Pro Phe Thr His Ser Gly Gly Asn Asn Asn Asn

100 105 110

Asn Glu Asp Asp Asp Gly Asp Asp Asn Asn Asn Lys Lys Ile Thr Asn

115 120 125

Lys Asn Asn Thr Asn Ser Ile Ile Asn Ser Asn Ser His Phe His Thr

130 135 140

Trp Ser Gly Ile Lys Arg Ser Ile Pro Ser Leu Ala Leu Phe Ala Phe

145 150 155 160

Leu Met Cys Cys Val Leu Ile Leu Met Thr Ser Thr Leu Leu His Gly

165 170 175

Ser Ser Thr Ser Phe Ser Thr Phe Lys Lys Asn Met Phe Asn Trp Arg

180 185 190

His Asn Asn Gly Val Ala Asn Ser Phe Leu Arg Phe Asn His Phe Asn

195 200 205

Asn Gly Pro Thr Tyr Asn Ile His Asn Thr Ser His Ile Ser Leu Ser

210 215 220

Asn Gly Asn Glu His Val Leu Glu Tyr Asn Pro Gln Tyr Arg Lys Ala

225 230 235 240

Ser Lys Lys Leu Tyr Glu Leu Leu Ser Asp Phe Asp Thr Ala Phe Tyr

245 250 255

Asp Asp Asn Asn Met Val Leu Gly Thr Asp Lys Phe Ser Gln Glu Asn

260 265 270

Thr Tyr Ser Arg Gln Pro Tyr Val Ser Asn Gly Tyr Ile Gly Ala Arg

275 280 285

Leu Pro Asn Ile Gly Phe Gly Phe Ser Leu Asp Thr Phe Asn Pro Trp

290 295 300

Thr Pro Asp Ser Lys Asn Ile Pro Asp Ser Leu Asn Asn Gly Trp Pro

305 310 315 320

Leu Arg Asn Arg Arg Phe Thr Gly Ala Tyr Val Ser Asp Phe Tyr Ser

325 330 335

Ile Gln Lys Ser Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp Glu Lys

340 345 350

Gly Tyr Ser Thr Val Ile Ser Thr Ile Pro Gln Trp Thr Asn Leu Gln

355 360 365

Phe Ser Ile Glu Phe Asn Asn Arg Ile His Trp Phe Asn Pro Gln Met

370 375 380

Val Lys Asp Glu Asp Ile Thr Asn Tyr Lys Gln Asn Leu Ser Met Lys

385 390 395 400

Asn Gly Val Val Thr Thr Glu Met Asp Tyr Leu Asp Ser Leu Val His

405 410 415

Ile Lys Ser Asp Val Trp Thr His Arg Arg Met Tyr Pro Leu Gly Val

420 425 430

Leu Asn Val Glu Ile Thr Leu Asn Thr Glu Lys Leu Asn Tyr Asn Asn

435 440 445

Asn Asn Asn Asn Asn Asn Asn Arg Asn Ser Asn Asp Glu Glu Tyr Asn

450 455 460

Ser Asp Glu Gln Val Ile Leu Thr Val Tyr Asp Thr Leu Asp Met Glu

465 470 475 480

Thr Ser Phe Arg Thr Phe Leu Gln Thr Ser Gly Ile Glu Glu Asp Asn

485 490 495

Asn Ala Ile Phe Met Val Val His Pro Asp Asn Ile Pro Tyr Ser Asn

500 505 510

Ala Ala Ile Tyr Ser Thr Cys Ser Ala His Tyr Gly Asn Ser Asn Gly

515 520 525

Trp Arg Thr Pro Lys Lys Asp Phe Ala Phe Lys Lys Asn Phe Ser Ser

530 535 540

Ile Gly Asn Asp Asp Asn Gly Glu Lys Ile Ser Gln Tyr Thr Lys Val

545 550 555 560

Asn Leu Ser Lys Tyr Asn Lys Lys Leu Ile Ile Asn Lys Tyr Ser Ala

565 570 575

Ile Ile Ser Ser Glu Phe Asn Ser Asn Glu Gln Lys Ser Asn Leu Glu

580 585 590

Met Ala Lys Glu Ile Ala Leu Ala Ser Lys Gly Asn Tyr Glu Ser Leu

595 600 605

Leu Lys Ser His Thr Glu Ala Trp Ala Glu Leu Tyr Asn Asp Ala Phe

610 615 620

Ile Glu Ile Pro Ser Asp Ser Leu Leu Glu Met Thr Ala Arg Ser Ser

625 630 635 640

Leu Phe His Ile Leu Ser Asn Thr Arg Lys Tyr Asn Val Ser Glu Glu

645 650 655

Arg Gly Leu Pro Val Pro Val Gly Gly Leu Ser Ser Asp Ser Tyr Gly

660 665 670

Gly Met Val Phe Trp Asp Ala Asp Ile Trp Met Gln Pro Ala Val Leu

675 680 685

Pro Phe Phe Pro Asn Ile Ala Lys Gln Met Ser Asn Tyr Arg Asn Ala

690 695 700

Thr His Gln Gln Ala Leu Leu Asn Ala Lys Glu His Gly His Pro Gly

705 710 715 720

Ala Leu Tyr Pro Trp Thr Ser Gly Arg Phe Ala Asn Cys Ser Ser Thr

725 730 735

Gly Pro Cys Tyr Asp Tyr Glu Tyr His Ile Asn Val Asp Val Ala Met

740 745 750

Ala Ser Phe Ser Ile Tyr Met Asn Gly Gly Glu Gly Ile Asp Asp Asp

755 760 765

Tyr Leu Arg Tyr Thr Thr Trp Pro Leu Val Arg Asp Ala Ala Ile Phe

770 775 780

Phe Ala Ser Phe Val Gln Phe Asn Gln Thr Ile Gly Lys Tyr Glu Thr

785 790 795 800

Tyr Asn Leu Thr Asp Pro Asp Glu Phe Ala Asn Phe Val Asn Asn Gly

805 810 815

Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp Ala Thr Asp

820 825 830

Ile Gly Asn His Leu Gly Glu Phe Val Asp Pro Arg Trp Met Glu Ile

835 840 845

Ser His Asn Ile Tyr Ile Pro Arg Ser Thr Ser Asn Ile Thr Leu Glu

850 855 860

Tyr Ser Gly Met Asn Ser Ser Val Glu Val Lys Gln Ala Asp Val Ile

865 870 875 880

Leu Met Val Tyr Pro Leu Gly Tyr Leu Asn Asp Glu Ser Ile Leu Asn

885 890 895

Asn Ala Ile Arg Asp Leu Tyr Tyr Tyr Ser Glu Lys Gln Ser Ala Ser

900 905 910

Gly Pro Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Thr Leu

915 920 925

Leu Asn His Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Leu

930 935 940

Pro Tyr Leu Arg Ala Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp

945 950 955 960

Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr

965 970 975

Ala Asn Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile

980 985 990

Arg Tyr Thr Tyr Glu Val Asp Gln Glu Thr Lys Gln Ile Tyr Arg Val

995 1000 1005

Leu Arg Phe Asn Pro Thr Glu Leu Pro Arg Leu Pro Gly Gly Ile

1010 1015 1020

Ala Ile Arg Asn Phe Lys Tyr Met Asn Gln Val Leu Asp Ile Ile

1025 1030 1035

Ile Asp Asp Asn Asn Gly Thr Ile Val His Lys Ser Gly Asp Thr

1040 1045 1050

Pro Ile Met Ile Lys Ile Pro Asp Arg Ser Leu Ile His Asp Arg

1055 1060 1065

Asp Val Glu Pro Tyr Arg Gly Ile Asn Lys Thr Asn Asn Leu Pro

1070 1075 1080

Val Glu Asp Ile Asn Asn Ser Ile Gln Val Asp Asp His Val Glu

1085 1090 1095

Ile Leu Gln Asn Gly Ser Tyr Tyr Ile Leu Asn Pro Lys Glu Glu

1100 1105 1110

Leu Lys Leu Pro Leu Phe Lys Leu Lys Leu Asn Val Lys Gly Asn

1115 1120 1125

Ile Val Glu Asn Lys Gln Ile Thr Asn Leu Thr Glu Gly Val Pro

1130 1135 1140

Gly Asp Val Ala Val Ser Ala Leu Asp Gly Asn Asn Tyr Thr His

1145 1150 1155

Trp Gln Pro Met Asp Lys Tyr Arg Pro Ala Arg Leu Leu Ile Asp

1160 1165 1170

Leu Gly Lys Asp Asn Gln Gln Glu Ile Lys Ser Gly Met Ile Leu

1175 1180 1185

Trp Gly Gly Arg Pro Ala Lys Asn Phe Ser Ile Ser Ile Leu Pro

1190 1195 1200

His Ser Asp Gln Ile Glu Arg Ile Leu Glu Lys Val Ser Asp Arg

1205 1210 1215

Leu Glu Ser Thr Glu Gly Ser Glu Lys Ser Arg Thr Ile Gln Glu

1220 1225 1230

Leu Leu Glu Asp Ile Gly Asp Val Lys Asp Thr Ser Met Glu Gly

1235 1240 1245

Cys Gln Asn Ser Asp Glu Asp Arg Cys Thr Asn Asp Leu Glu Ser

1250 1255 1260

Ile Leu Gln Trp Thr Leu Asp Asp Leu Asp Asn Ile Leu Ser Asn

1265 1270 1275

Leu Pro Gln Leu Lys Leu Leu Asn Arg Lys Phe Ile Thr Ile Leu

1280 1285 1290

Lys Asp His Glu Val Ile Pro Ser Glu Pro Phe Phe Pro Glu Ile

1295 1300 1305

Ile Glu Gln Ser Leu Ile Glu Ile Leu Pro Ser Asn Lys Thr Tyr

1310 1315 1320

Phe His Ile Asp Tyr Ser Lys Ile Lys Ile Gly Ser Asn Gly Lys

1325 1330 1335

Ser Leu Glu Asp Asn Glu Glu Asp Arg Lys Ala Arg Phe Ile Val

1340 1345 1350

Leu Thr Val Asn Gly Val Phe Asp Asp Asp Asn Asp Ser Lys Gly

1355 1360 1365

Ala Thr Ile Lys Glu Ile Val Leu Lys Asn Asp Asp Thr Glu Phe

1370 1375 1380

Ile Asp Ile

1385

<210> 46

<211> 1170

<212> PRT

<213> 鲁氏接合酵母(Zygosaccharomyces rouxii)

<400> 46

Met Met Lys Ile Asp Leu Asn Ala Asp Ala Gly Asp Asp Ser Gln Val

1 5 10 15

Pro Leu Arg Arg Glu Cys Gln Glu Ser Pro Lys Ala Val Arg Lys Lys

20 25 30

Val Ile Ser Asn Ile Ile Met Ser Val Leu Ala Ile Phe Gly Leu Leu

35 40 45

Leu Leu Leu Gly Trp Gln Thr Ala Lys Gly Gln Ala Val Leu Pro Ala

50 55 60

Ala Gln Gly Pro Gly Ile Ile Ala Lys Ala Thr Arg Ser His Asp Leu

65 70 75 80

Gln Thr Asn Asp Glu Val Arg Lys Ala Ser Lys Lys Leu Tyr Glu Leu

85 90 95

Leu Ala Asp Thr Glu Gly Ala Tyr Tyr Asp Pro Asp Ala Met Val Leu

100 105 110

Gly Ser Thr Ile Phe Ser Pro His Thr Tyr Ser Arg Gln Pro Tyr Val

115 120 125

Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Val Gly Phe Gly Tyr

130 135 140

Ala Glu Asp Val Trp Asn Ile Trp Ala Asn Ala Asp Asp Lys Ile Pro

145 150 155 160

Gly Ala Leu Glu Asn Gly Trp Pro Leu Arg Asn Arg Arg Phe Ala Gly

165 170 175

Ala Phe Val Ser Asp Phe Tyr Cys Leu Gln Glu Ser Leu Asn Ser Thr

180 185 190

Asn Phe Pro Glu Leu Asp Lys Asp Gly Tyr Ser Thr Val Ile Ala Ser

195 200 205

Ile Pro Gln Trp Thr Gln Leu Gln Phe Ser Leu Asn Asp Glu Lys Trp

210 215 220

Phe Asp Pro Gln Ser Val Asp Ser Glu Asp Val Ser Asn Tyr Thr Gln

225 230 235 240

Glu Leu Ser Met Lys Asn Gly Val Val Thr Thr Cys Leu Asp Trp Leu

245 250 255

Asp Gly Leu Leu His Val Glu Thr Glu Val Leu Ala His Lys Lys Ile

260 265 270

Tyr Thr Leu Gly Ile Val Ser Met Lys Val Ser Leu Asn Val Asp Arg

275 280 285

Leu Pro Glu Asp Glu Ser Ser Val Glu Leu Asn Phe Trp Asp Thr Leu

290 295 300

Asp Phe Asn Thr Ser His Arg Ser Val Leu Ala Gly Lys Gly Tyr Asp

305 310 315 320

Glu Gly Leu Tyr Met Val Val Glu Pro Glu Asn Val Pro Tyr Ser Asn

325 330 335

Ala Ser Ile Phe Ser Lys Cys Glu Val Asp Gly Ser Lys Val Glu Phe

340 345 350

Ser Glu Phe Gly Ser His Glu Gln Lys Val Thr Gln Asn Ala Ser Val

355 360 365

Ile Leu Thr Arg Asp Asn Pro Gln Thr Thr Ile His Lys Tyr Val Gly

370 375 380

Ile Val Ser Thr Glu Tyr Ser Asp Gly Ala Thr Leu Ser Arg Ala Lys

385 390 395 400

Asp Val Ala Ile Ala Ser His Gly Asn Tyr Glu Lys Leu Ser Leu Ala

405 410 415

His Asp Glu Ala Trp Lys Lys Ile Tyr Asp Asn Ala Phe Ile Glu Ile

420 425 430

Pro Ser Asp Glu Val Leu Glu Met Thr Ala Arg Ser Ser Leu Tyr His

435 440 445

Ile Leu Ala Asn Thr Arg Thr Tyr Asn Val Ser Glu Asn Arg Gly Leu

450 455 460

Pro Val Ser Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val

465 470 475 480

Phe Trp Asp Ser Asp Phe Trp Ile Gln Pro Gly Ile Leu Pro Phe Phe

485 490 495

Pro Asp Ile Ala Lys Asn Ile Asn Asn Tyr Arg Asn Ala Thr His Glu

500 505 510

Gln Ala Arg Leu Asn Ala Glu Gln Tyr Gly Tyr Pro Gly Ala Leu Tyr

515 520 525

Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys

530 535 540

Val Asp Tyr Glu Tyr His Ile Asn Val Asp Val Cys Leu Ala Ser Phe

545 550 555 560

Ser Ile Tyr Leu Asn Gly Ala Glu Gly Ile Asp Asp Glu Tyr Leu Lys

565 570 575

Tyr Thr Thr Trp Pro Met Val His Asp Ala Ala Glu Phe Phe Thr Ala

580 585 590

Phe Val Lys Tyr Asn Ser Thr Ile Asp Arg Tyr Glu Thr His Asn Leu

595 600 605

Thr Asp Pro Asp Glu Phe Ala Asn His Ile Asp Asn Gly Ala Phe Thr

610 615 620

Asn Ala Gly Ile Lys Leu Leu Leu Lys Trp Ala Thr Asp Ile Gly Tyr

625 630 635 640

His Ile Arg Glu Pro Val Asp Pro Arg Trp Met Glu Ile Ser Glu Lys

645 650 655

Ile Tyr Ile Pro Gln Ser Asp Ser Asn Ile Thr Leu Glu Tyr Ser Gly

660 665 670

Met Asn Ser Ser Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val

675 680 685

Tyr Pro Leu Ala Phe Ile Thr Asp Glu Ser Ile Leu Asn Asn Ala Ile

690 695 700

Lys Asp Val Tyr Tyr Tyr Ser Glu His Gln Ser Ser Ser Gly Pro Ala

705 710 715 720

Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Gly Ile Leu Asn His

725 730 735

Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Leu Leu Pro Tyr Leu

740 745 750

Arg Ala Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Met

755 760 765

Thr Asn Gly Lys Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly

770 775 780

Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile Arg Tyr Ser

785 790 795 800

Tyr Glu Val Glu Pro Gly Thr Lys Lys Ile Gln Arg Ile Leu Lys Phe

805 810 815

Asn Pro Ile Glu Leu Pro Leu Leu Pro Gly Gly Leu Ala Ile Arg Asn

820 825 830

Phe Lys Tyr Leu Asp Gln Val Leu Asp Ile Ile Ile Asp Asp Arg Asn

835 840 845

Gly Thr Ile Val His Lys Ser Gly Asp Gln Pro Ile Arg Ile Lys Val

850 855 860

Pro Asp Arg Thr Lys Ile His Asp Arg Asp Ile Thr Pro Tyr Arg Gly

865 870 875 880

Asn Gly Ser Thr Gly Met Glu Ser Phe Gln Asn Glu Asp Ala Thr His

885 890 895

Gly Thr Ser Ala His Tyr Tyr Thr Ile Asn Pro Gly Glu Glu Leu Ile

900 905 910

Leu Pro Leu Phe His Met Ala Pro Asn Val Glu Gly Asn Leu Val Glu

915 920 925

Ser Lys Gln Val Thr Asn Leu Thr Ala Gly Val Pro Gly Asp Val Ala

930 935 940

Ile Ser Thr Leu Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Val Asp

945 950 955 960

Lys Ser Leu Pro Gly Lys Leu Leu Ile Asp Leu Gly Ala Asn Asn Ser

965 970 975

Gln Val Ile Asn Arg Gly Met Ile Leu Trp Gly Gln Arg Pro Ala Lys

980 985 990

Asn Phe Thr Ile Ser Ile Leu Pro His Thr Glu Asp Met Gly Gln Val

995 1000 1005

Leu Ser Asn Leu Thr Thr Ile Val Glu Ser Thr Asn Val Gly Asp

1010 1015 1020

Glu Ser Val Leu Glu Ala Leu Glu Asp Ile Gly Gly Leu Ser Pro

1025 1030 1035

Leu Leu Ser His Ser Gly Asp Glu Asp Val Lys Asn Leu Leu Ser

1040 1045 1050

Trp Lys Ala Glu Asp Val Asp Asp Ile Met Leu Gly Ile Ser Gly

1055 1060 1065

Glu Ser Asn Val Ile Arg Gly Lys Phe Val Pro Ile Leu Lys Asp

1070 1075 1080

Tyr Lys Val Glu Pro Asn Glu Pro Tyr Phe Glu Glu Ile Tyr Ser

1085 1090 1095

Lys Ser Ile Ile Glu Ile Leu Pro Ser Asn Arg Thr Glu Phe Thr

1100 1105 1110

Ile Asp Tyr Asp Lys Val Gln His Asn Ser Thr Thr Cys Ser Tyr

1115 1120 1125

Lys Asn Leu Ser Asp Asn Cys Thr Asp Trp Thr Lys Thr Arg Phe

1130 1135 1140

Ile Met Val Ser Ile Glu Gly Thr Tyr Asp Asn Asp Thr Asp Asp

1145 1150 1155

Lys Gly Gly Thr Ile Lys Glu Ile Val Leu Leu Asn

1160 1165 1170

<210> 47

<211> 1175

<212> PRT

<213> 副贝氏丝酵母(Zygosaccharomyces parabailii)

<400> 47

Met Lys Arg Asp Leu Thr Arg Asn Pro Ser Asn Val Ser Thr Ala Ala

1 5 10 15

Glu His His Ala Lys Glu Pro Lys Asp Cys Gly Ile Lys Thr Arg Leu

20 25 30

Leu Gly Val Ser Met Val Leu Phe Thr Ile Phe Thr Phe Phe Leu Leu

35 40 45

Leu Gly Trp Pro Phe Gly Gly Leu Ala Pro Thr Phe Ser Ala Ala Glu

50 55 60

Ser Ala Gly Ile Ile Arg Lys Thr Ser Asp Val His Thr Leu Asp Phe

65 70 75 80

Asn Lys Glu Ala Arg Arg Ala Ser Lys Lys Leu Tyr Glu Leu Leu Val

85 90 95

Asp Ile Asp Gly Ala Phe Tyr Asp Ala Glu Asn Met Val Leu Gly Ala

100 105 110

Thr Lys Phe Ser Pro His Thr Tyr Ser Arg Gln Pro Tyr Val Ala Asn

115 120 125

Gly Tyr Ile Gly Ser Arg Ile Pro Asn Val Gly Ala Gly Tyr Ala Glu

130 135 140

Asp Thr Trp Asn Ile Trp Ala Ser Ala Gly Glu Glu Leu Pro Gly Ala

145 150 155 160

Leu Asn Asn Gly Trp Pro Leu Arg Asn His Arg Phe Ala Gly Ala Phe

165 170 175

Val Ser Asp Phe Tyr Cys Leu Glu Lys Thr Leu Asn Ser Thr Asn Phe

180 185 190

Pro Glu Leu Asp Glu Asn Gly Tyr Ser Thr Val Ile Ser Ser Ile Pro

195 200 205

Gln Trp Thr Asn Leu Gln Phe Ser Leu Asn Glu Thr Val Trp Phe Asn

210 215 220

Pro Gln Ser Val Thr Asn Asp Asn Val Thr Asn Tyr Arg Gln Glu Leu

225 230 235 240

Ser Met Lys Asn Gly Val Val Thr Thr His Leu Asp Cys Phe Asp Glu

245 250 255

Lys Val His Val Glu Thr Gln Ile Phe Ala His Lys Lys Ile Tyr Pro

260 265 270

Leu Gly Ile Val Ser Met Lys Val Ala Leu Asn Ile Asp His Leu Ile

275 280 285

Pro Gly Gln Phe Ser Ile Glu Leu Asn Phe Trp Asp Ile Leu Asp Phe

290 295 300

Asn Thr Ser His Arg Thr Val Leu His Asp Leu Gly Tyr Asp Asp Gly

305 310 315 320

Ile Phe Met Ile Val Glu Pro Asp Asn Val Pro Tyr Ser Asn Ala Ser

325 330 335

Ile Phe Ser Thr Cys Thr Val Asp Arg Thr Gly Val Thr Phe Asp Arg

340 345 350

Gly Glu Thr Thr Tyr Glu Gln Ile Ser Gln Asn Ala Ser Val Ile Leu

355 360 365

Thr Lys Asp Asn Pro Asn Val Thr Ile His Lys Tyr Val Gly Ile Val

370 375 380

Ser Ser Glu Tyr Tyr Asn Gly Ser Thr Leu Asp Tyr Ala Lys Glu Ile

385 390 395 400

Ala Lys Ser Asn Arg Gly Asn Tyr Glu Lys Val Ser Lys Val His Asn

405 410 415

Glu Ala Trp Asp Ala Leu Tyr Asn Asp Ala Phe Ile Glu Ile Pro Ser

420 425 430

Asp Glu Val Leu Glu Met Thr Thr Arg Ser Ser Leu Tyr His Leu Leu

435 440 445

Ala Asn Thr Arg Thr Tyr Asn Val Ser Glu Ala Arg Gly Leu Pro Val

450 455 460

Pro Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp

465 470 475 480

Asp Ala Asp Phe Trp Ile Gln Pro Ser Val Leu Pro Phe Phe Pro Asp

485 490 495

Ile Ala Lys Asn Ile Asn Asn Tyr Arg Asn Ala Thr His Arg Gln Ala

500 505 510

Gln Leu Asn Ala Gln Gln His Gly Tyr Pro Gly Ala Ile Tyr Pro Trp

515 520 525

Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Met Asp

530 535 540

Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ala Ser Phe Ser Ile

545 550 555 560

Tyr Leu Asn Gly Ala Gln Gly Ile Asp Asp Thr Tyr Leu Lys Tyr Thr

565 570 575

Thr Trp Pro Ile Val Arg Asp Ala Ala Glu Phe Phe Thr Asp Phe Val

580 585 590

Lys Tyr Asn His Thr Ile Gly Lys Tyr Glu Thr His Asn Leu Thr Asp

595 600 605

Pro Asp Glu Phe Ala Asn His Ile Asn Asn Gly Ala Phe Thr Asn Ala

610 615 620

Gly Ile Arg Leu Leu Leu Lys Trp Ala Thr Asp Ile Gly His His Leu

625 630 635 640

Arg Glu Pro Val Asp Pro Arg Trp Met Asn Ile Ser Glu Asn Ile Tyr

645 650 655

Ile Pro Ile Ser Glu Ser Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn

660 665 670

Ser Ser Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val Tyr Pro

675 680 685

Leu Gly Tyr Ile Thr Asp Glu Leu Val Leu Asn Asn Ala Ile Lys Asp

690 695 700

Leu Tyr Tyr Tyr Ser Glu His Gln Ser Pro Ser Gly Pro Ala Met Thr

705 710 715 720

Tyr Pro Val Phe Val Ala Ser Ala Ala Gly Met Leu Asn His Gly Ser

725 730 735

Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Leu Leu Pro Tyr Leu Arg Ala

740 745 750

Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn

755 760 765

Gly Lys Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Phe

770 775 780

Leu Gln Ser Val Leu Phe Gly Leu Thr Gly Ile Arg Tyr Thr Tyr Glu

785 790 795 800

Val Asn Pro Ala Ser Lys Lys Ile Glu Arg Val Leu Lys Phe Asn Pro

805 810 815

Ile Glu Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn Phe Lys

820 825 830

Tyr Met Asn Gln Val Leu Asp Ile Ile Ile Asp Asp His Asn Gly Thr

835 840 845

Ile Val His Lys Ser Gly Asp Lys Ser Ile Arg Ile Lys Val Pro Asp

850 855 860

Arg Ser Leu Ile Arg Asp Arg Asp Val Asn Pro Tyr Arg Gly Pro Asn

865 870 875 880

Ser Ser Gln Glu Val Val Asn Glu Lys Gly His Asp Val Glu Ala Leu

885 890 895

Gly Asp Lys Ser Leu Ala Arg Lys Ala Gln Tyr Tyr Thr Leu Asn Pro

900 905 910

Gly Glu Glu Leu Ile Leu Pro Leu Phe Lys Met Lys Lys Asn Val Glu

915 920 925

Gly Asn Ile Val Glu Ser Lys Gln Ile Thr Asn Leu Thr Gly Gly Val

930 935 940

Pro Gly Asp Val Ala Phe Ser Ala Leu Asp Gly Asn Asn Tyr Thr His

945 950 955 960

Trp Gln Pro Val Asp Lys Ser Ala Pro Ala Lys Leu Leu Ile Asp Leu

965 970 975

Gly Gln Asn Asn Ser Gln Val Ile Gln Lys Gly Met Ile Leu Trp Gly

980 985 990

Gln Arg Pro Ala Lys Asn Ile Thr Ile Ser Ile Leu Pro His Thr Glu

995 1000 1005

Thr Val Gly Gln Ile Leu Glu Glu Leu Thr Thr Ile Ala Glu Asn

1010 1015 1020

Val Asn Val Gly Glu Glu Ser Val Met Glu Ala Leu Glu Asp Val

1025 1030 1035

Gly Gly Thr Asp Phe Leu Leu Ala Asn His Gly Asp Gln Asp Val

1040 1045 1050

Lys Asp Leu Leu Ser Trp Arg Ala Asp Asp Leu Glu Glu Ile Val

1055 1060 1065

Arg Gln Leu Ser Asn Asp Ser Asn Val Met Arg Gly Lys Phe Val

1070 1075 1080

Pro Ile Leu Glu Asn Tyr Pro Val Val Pro Thr Val Pro Tyr Phe

1085 1090 1095

Arg Glu Ile Tyr Asp Lys Ser Ile Ile Gln Ile Leu Pro Ser Asn

1100 1105 1110

Ile Thr Glu Phe Thr Ile Asp Tyr Ser Lys Ile Ala His Asn Ser

1115 1120 1125

Ser Arg Cys Ser Tyr Arg Asn Ser Ser Asn Asn Cys Thr Asp Trp

1130 1135 1140

Lys Lys Thr Arg Phe Ile Leu Leu Gly Ile Gln Gly Ser Tyr Asp

1145 1150 1155

Glu Asp Leu Asp Pro Lys Gly Gly Thr Ile Lys Glu Ile Val Leu

1160 1165 1170

Leu Asn

1175

<210> 48

<211> 1202

<212> PRT

<213> 毕赤四盾酵母(Tetrapisispora phaffii)

<400> 48

Met Phe Phe Asp Asn Val Tyr Ser Gln Arg Lys Glu Lys Asp Leu Val

1 5 10 15

Leu Leu Ile Trp Phe Gln Arg Ile Phe Ile Ile Met Leu Leu Ile Ile

20 25 30

Phe Leu Ile Phe Asn Cys Phe Ile Thr Val Gln Ser Ile His Ile Asn

35 40 45

Ser Phe Phe Asn Asn Lys Phe Leu Arg His Phe Ser Thr Arg Asn Asn

50 55 60

Lys Leu Ile Tyr Ala Ser Pro Ile Ala Gln Ser Asn Leu Ser Ile Leu

65 70 75 80

Ser Val Gly Glu Glu Ile Ser Gln Val Ala Thr Ala Arg Lys Thr Ser

85 90 95

Lys Lys Leu Tyr Asp Leu Leu Ile Asn Tyr Glu Asp Ser Tyr Tyr Asp

100 105 110

Asp Glu Asn Met Val Leu Gly Thr Asp Val Ile Thr Lys Asn Thr Tyr

115 120 125

Thr Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro

130 135 140

Asn Ile Gly Phe Gly Tyr Ala Leu Asp Glu Tyr Ser Ile Trp Asn Pro

145 150 155 160

Asp Val Thr Asp Asn Pro Glu Leu Phe Asn Asn Gly Trp Pro Leu Arg

165 170 175

Asn Arg Arg Tyr Ala Gly Ser Phe Val Ser Asp Phe Tyr Ser Leu Gln

180 185 190

Lys Lys Leu Asn Ser Thr Asn Phe Pro Glu Ile Asp Asp Ile Gly Tyr

195 200 205

Ser Asn Val Ile Ser Ser Ile Pro Glu Trp Thr Asn Ile Lys Leu Thr

210 215 220

Val Ile Asp Glu Asn Asp Arg Ser Phe Asn Phe Asn Pro Gln Asp Ile

225 230 235 240

Ser Val Asn Asp Ile Thr Asn Tyr His Gln Asn Leu Ser Met Gln Asn

245 250 255

Gly Met Val Thr Thr Asp Leu Asn Trp Leu Asn Asn Thr Leu His Ile

260 265 270

Asn Ser Thr Met Phe Thr His Arg Lys Leu Tyr Pro Leu Gly Leu Leu

275 280 285

Glu Ile Asp Ile Thr Leu Asn Arg Glu Tyr Leu Pro Thr Asp Phe Ala

290 295 300

Thr Tyr Asn Ile Ser Val Phe Asp Ile Leu Asp Phe Asn Thr Ser His

305 310 315 320

Arg Thr Phe Leu Arg Asp Met Gly Pro Ser Tyr Asp Ser Ser Lys Met

325 330 335

Gln Gly Ile Phe Met Val Val Glu Pro Glu Asn Val Pro Tyr Thr Asn

340 345 350

Cys Val Ile Phe Ser Thr Thr Glu Ile Glu Gln Leu Asp Ile Thr Gly

355 360 365

Ser Val His Arg Asn Ile Thr Leu Asp Asp Tyr Val Ile Asp Gly Met

370 375 380

Asn Ser Ser Ile Ser Gln Val Ala Lys Asp Ile Thr Leu Ser Thr Glu

385 390 395 400

Phe Pro Lys Leu Lys Ile Tyr Lys Tyr Ser Ser Ile Ile Ser Ser Asn

405 410 415

Tyr Asp Gly Cys Lys Asn Asn Thr Tyr Gln Asp Asn Leu Asn Glu Ala

420 425 430

Lys His Leu Ala Leu Asn Ser Arg Gly Asn Phe Gln Asn Leu Ala Asp

435 440 445

Ser Asn Thr Gln Ala Trp Val Asp Leu Tyr Asp Asn Ala Phe Ile Glu

450 455 460

Ile Pro Ser Asp Ser Val Leu Glu Leu Ala Ala Arg Ser Ser Leu Phe

465 470 475 480

His Leu Met Ser Asn Ala Arg Leu His Asn Ile Ser Glu Asp Arg Gly

485 490 495

Leu Pro Leu Pro Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Leu

500 505 510

Val Phe Trp Asp Ala Asp Val Trp Met Gln Pro Ala Ile Leu Pro Phe

515 520 525

Phe Pro Asp Val Ala Lys Asn Ile Asn Asn Tyr Arg Asn Ala Thr His

530 535 540

Lys Gln Ala Gln Leu Asn Ala Lys Gln Tyr Gly Tyr Asn Gly Ala Trp

545 550 555 560

Tyr Pro Trp Thr Ser Gly Gly Phe Ser Asn Cys Thr Ser Thr Gly Pro

565 570 575

Cys Ile Asp Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ala Thr

580 585 590

Phe Ser Ile Tyr Met Asn Gly Ala Asp Gly Ile Asp Asp Thr Tyr Leu

595 600 605

Lys Tyr Thr Val Trp Pro Ile Val Arg Asp Ala Ala Ile Ala Phe Thr

610 615 620

Glu Tyr Val Gln Tyr Asp Lys Gln Leu Asp Val Tyr Glu Thr Phe Asn

625 630 635 640

Leu Thr Asp Pro Asp Glu Phe Ala Asn His Val Asn Asn Gly Ala Phe

645 650 655

Thr Asn Ala Gly Ile Lys Ile Leu Met Lys Trp Ala Val Asp Ile Gly

660 665 670

Asn His Leu Glu Glu Lys Val Asp Pro Ile Trp Lys Ala Ile Asn Glu

675 680 685

Lys Ile Val Ile Pro Lys Thr Ser Ser Asn Ile Thr Leu Glu Tyr Ser

690 695 700

Gly Met Asn Ser Thr Ala Glu Ile Lys Gln Ala Asp Val Pro Leu Leu

705 710 715 720

Val Tyr Pro Leu Gly Tyr Ile Thr Asp Glu Ser Ile Leu Asn Asn Ala

725 730 735

Ile Arg Asp Leu Tyr Tyr Tyr Ser Glu His Gln Ala His Thr Gly Pro

740 745 750

Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Gly Leu Leu Asn

755 760 765

His Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Ile Leu Pro Tyr

770 775 780

Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe

785 790 795 800

Leu Glu Asn Gly Gly Leu His Pro Ala Tyr Pro Phe Leu Thr Ala Asn

805 810 815

Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile Arg Tyr

820 825 830

Ser Tyr Lys Val Asp Ser Asp Thr Asn Lys Ile Ala Arg Leu Leu Lys

835 840 845

Phe Asn Pro Ile Lys Leu Lys Leu Leu Pro Gly Gly Leu Lys Ile Arg

850 855 860

Asn Phe Lys Tyr Met Gly Gln Val Leu Asp Ile Ile Ile Asp Asp Tyr

865 870 875 880

Asn Gly Thr Ile Ile His Lys Ser Gly Asn Lys Thr Ile Thr Ile Gln

885 890 895

Ile Pro Asp Arg Ser Ser Ile Arg Asp Gln Asp Ile Asn Pro Tyr Arg

900 905 910

Gly Lys Asn Lys Thr Lys Glu Asp Asn Arg Ile Leu Ser Lys Arg Lys

915 920 925

Ser Asn Ile Tyr Glu Leu Lys Pro Asn Glu Asn Phe Thr Ile Pro Leu

930 935 940

Phe Val Thr Glu Leu Asn Ala Pro Gly Asn Ile Val Glu Ser Lys Gln

945 950 955 960

Ile Thr Asn Leu Thr Glu Gly Val Ala Gly Asp Val Ala Met Ser Ala

965 970 975

Val Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Val Asn Lys Ala Glu

980 985 990

Thr Ala Arg Leu Leu Ile Asp Leu Gly Pro Gly Asn Glu Gln Thr Ile

995 1000 1005

Thr Gly Gly Met Phe Leu Trp Gly Gln Arg Pro Ala Lys Asn Val

1010 1015 1020

Ser Ile Ser Val Leu Pro His Ser Gln Ala Thr Glu Gln Leu Phe

1025 1030 1035

Gln Asn Ile Thr Tyr Phe Ala Glu His Phe Glu Phe Pro Asn Arg

1040 1045 1050

Glu Arg Thr Ile Leu Gln Leu Ile Gln Glu Val Asp Asn Ile Thr

1055 1060 1065

Glu Pro Glu Val Cys Gln Ile Phe Ser Ser Asp Gly Val Cys Asp

1070 1075 1080

Ala Gly Phe Thr Gln Leu Leu Asn Trp Lys Ser Lys Asp Ile Asp

1085 1090 1095

Glu Leu Ile Gln Gln Phe Pro Asn Thr Pro Gly Ile Arg Glu Asn

1100 1105 1110

Phe Ile Thr Ile Val Asp Asn Leu Ser Val Leu Pro Ser Glu Pro

1115 1120 1125

Tyr Tyr Pro Glu Tyr Tyr Glu Gln Ala Leu Ile Glu Ile Leu Pro

1130 1135 1140

Ser Asn Lys Thr Lys Phe Ser Val Asp Tyr Ser Lys Val Glu Tyr

1145 1150 1155

Tyr Lys Lys Ser Glu Val Phe Glu Gln Ser Gln Trp Pro Lys Thr

1160 1165 1170

Arg Phe Ile Ile Val Ser Ile Gln Asp Thr Tyr Asp Lys Asp Leu

1175 1180 1185

Asp Pro Lys Gly Ser Thr Ile Lys Glu Ile Ile Leu Tyr Ser

1190 1195 1200

<210> 49

<211> 1180

<212> PRT

<213> 棉假囊酵母(Eremothecium gossypii)

<400> 49

Met Ala Asp Thr Ala Ser Leu Pro Pro Gln Arg Asp Ser Ala Leu Gly

1 5 10 15

Met His Gly Pro His Gly Gly Leu Tyr Met Pro Val Ala Gln Gly Pro

20 25 30

Leu Gln Ala His Ala Ser Pro Arg Leu Val Ser Val Arg Met Val Leu

35 40 45

Ser Ser Ile Thr Ala Leu Ala Leu Val Ala Val Val Thr Val Leu Gly

50 55 60

Thr Ala Gln Pro Ala Arg Pro Thr Ala Pro Leu Ala Ala Ala Asp Glu

65 70 75 80

Gln Phe Trp Val Ala Gln His Arg Ser Ala Ser Lys Gln Leu Tyr Gln

85 90 95

Leu Val His Gly Ser Glu Leu Ser Phe Tyr Asp Glu Gly Arg Asp Val

100 105 110

Leu Gly Thr Thr Glu Leu Ser Arg Asn Met Tyr Ser Arg Gln Pro Tyr

115 120 125

Val Ala Asn Gly Tyr Ile Gly Ser Arg Val Pro Asn Val Gly Phe Gly

130 135 140

Tyr Ala Ala Asp Glu Glu Asn Ile Trp Thr Asp Ala Ser Val Pro Gly

145 150 155 160

Ala Leu Asn Asn Gly Trp Pro Leu Arg Asn Pro Arg Tyr Ala Gly Ser

165 170 175

Phe Val Ser Asp Phe Tyr Ser Leu Gln Ala Arg Leu Asn Ser Thr Asn

180 185 190

Phe Pro Glu Leu Asp Glu Glu Gly Tyr Ser Thr Val Ile Ala Ser Ile

195 200 205

Pro Glu Trp Thr Asp Leu Arg Val Arg Ala Asp Gly Ala Glu Leu Gly

210 215 220

Ala Glu Thr Val Ala Leu Glu Asp Met Gly Gly Tyr Val Gln Asn Met

225 230 235 240

Ser Leu Ala Asp Gly Val Val Thr Thr Glu Tyr Val Trp Arg Gly Leu

245 250 255

Ser Val Arg Ala Thr Val Ala Ala His Arg Ser Glu Tyr Pro Leu Gly

260 265 270

Leu Val Gln Leu Glu Val Ala Leu Cys Gly Asp Thr Glu Pro Arg Glu

275 280 285

Val Glu Val Arg Asp Val Leu Asn Phe Thr Thr Ser His Arg Thr Val

290 295 300

Leu Arg Glu Ala Gly His Asp Glu Asp Gly Ile Tyr Met Arg Val Glu

305 310 315 320

Pro Glu Asn Val Pro Tyr Ser Glu Ala Ala Leu Tyr Ser Val Phe Glu

325 330 335

Val Arg Gly Gly Glu Gly Ser Val Gln Pro Glu Arg Ala Ala Ala Gly

340 345 350

Ala Thr Val Ala Gln Trp Val Arg Val Arg Leu Thr Ala Ala Gln Pro

355 360 365

Arg Val Val Val Arg Lys Tyr Val Gly Val Val Ser Ser Glu Tyr Asn

370 375 380

Thr Ala Gly Gly Ser Asn Leu Glu Ala Ala Arg Ala Ala Ala Leu Ala

385 390 395 400

His Tyr Gly Ala Phe Asp Gly Ala Leu Val Ser His Arg Ala Ala Trp

405 410 415

Ser Ala Leu Tyr Gly Asn Ala Ser Ile Glu Ile Pro Ser Asp Phe Leu

420 425 430

Leu Glu Leu Ala Ala Lys Ser Ser Met Phe His Met Leu Ala Asn Thr

435 440 445

Arg Ala His Asn Val Ser Ala Thr Arg Gly Leu Pro Val Pro Val Thr

450 455 460

Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Ser Asp

465 470 475 480

Val Trp Met Leu Pro Gly Leu Leu Pro Phe Phe Pro Asp Ile Ala Arg

485 490 495

Glu Ile Ser Asn Tyr Arg Asn Ala Thr His Ala Gln Ala Val Ala Asn

500 505 510

Ala Arg His Tyr Asn Tyr Ser Gly Ala Leu Tyr Pro Trp Thr Ser Gly

515 520 525

Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Val Asp Tyr Glu Tyr

530 535 540

His Ile Asn Ile Asp Ile Ala Met Ser Ser Leu Ser Ile Tyr Met Asn

545 550 555 560

Gly Ala Asp Gly Ile Gly Glu Asp Tyr Leu Arg Tyr Thr Thr Trp Pro

565 570 575

Leu Leu Arg Asp Ala Ala Leu Phe Phe Thr Glu Tyr Val Arg Tyr Asn

580 585 590

Glu Thr Leu Asp Ala Tyr Thr Thr His Asn Leu Thr Asp Pro Asp Glu

595 600 605

Phe Ala Asn Phe Ile Asp Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys

610 615 620

Ile Leu Leu Arg Trp Ala Ile Asp Val Gly Thr His Leu Glu Glu Pro

625 630 635 640

Val Asp Thr Lys Trp Gln Glu Ile Ser Asp Lys Ile His Ile Pro Thr

645 650 655

Ser Glu Thr Asn Ile Thr Leu Glu Tyr Thr Gly Met Asn Ala Thr Val

660 665 670

Asp Ile Lys Gln Ala Asp Val Leu Leu Met Val Tyr Pro Leu Gly Tyr

675 680 685

Ile Thr Asp Glu Ser Ile Leu Asn Asn Ala Ile Gln Asn Leu Tyr Tyr

690 695 700

Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro Val

705 710 715 720

Phe Ala Ala Ala Ala Ala Thr Leu Leu Asn His Gly Ser Ser Ser Gln

725 730 735

Ser Tyr Leu Tyr Lys Ala Val Val Pro Tyr Leu Arg Ala Pro Phe Phe

740 745 750

Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu Thr

755 760 765

Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Tyr Leu Gln Ser

770 775 780

Leu Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr Thr Val Asn Pro

785 790 795 800

Glu Thr Lys Lys Met Glu Arg Leu Leu Lys Phe Ser Pro Val Arg Met

805 810 815

Pro Leu Leu Pro Gly Gly Ile Arg Ile Asn Asn Phe Lys Tyr Leu Gly

820 825 830

Gln Val Leu Asp Ile Ser Ile Asp Asp His Asn Ala Thr Ile Ala His

835 840 845

Lys Gln Gly Asn Thr Pro Ile His Ile Lys Val Pro Asp Arg Ser Ile

850 855 860

Leu Arg Asp Arg Asp Val Pro Val Tyr Lys Gly Ser Ala Leu Gln Ala

865 870 875 880

Arg Asp Val Ile Pro Tyr His Glu Leu Ser Asn Ser Asn Tyr Phe Thr

885 890 895

Val Asn Pro Gly Glu Thr Leu Thr Leu Pro Val Tyr Glu Pro Glu Leu

900 905 910

Asn Ile Gln Gly Asn Ile Val Glu Gly Arg Gln Ile Thr Asn Leu Thr

915 920 925

Gln Gly Val Pro Gly Asp Val Pro Ile Ser Ile Leu Asp Gly Asn Asn

930 935 940

Tyr Thr His Trp Gln Pro Phe Asp Lys Ser Glu Arg Ala Leu Leu Leu

945 950 955 960

Ile Asp Leu Gly Ser Glu Glu Glu Tyr Glu Ile Thr Thr Gly Lys Ile

965 970 975

Leu Trp Gly Ala Arg Pro Ala Lys Asn Phe Ser Ile Ser Ile Leu Pro

980 985 990

Asn Ser Lys His Ile Thr Glu Ile Leu Thr Lys Leu Thr Ala Met Met

995 1000 1005

Asp Gly Arg Asn Thr Asp Leu Val Ser Cys Ser Lys Cys His Ala

1010 1015 1020

Val Ser Ser Ser Gln His Leu Leu Gly Gly Leu Ala Asn Val Thr

1025 1030 1035

Asp Ser Lys Gly Leu Ala Ala Ile Asp Gly Glu Thr Val Asp Met

1040 1045 1050

Gly Ile Arg Glu Ile Phe Arg Trp Asn Leu Phe Asp Leu Pro Thr

1055 1060 1065

Ile Ser Ser Ile Ile Pro Glu Ala Ala Asn Ile Ser Glu Ser Phe

1070 1075 1080

Val Thr Val Leu Glu Asn Tyr Gln Val Thr Pro Ser Glu Pro Tyr

1085 1090 1095

Tyr Glu Glu Val Val Arg Lys Ser Gln Ile Val Ile Leu Pro Ser

1100 1105 1110

Asn Glu Thr Asp Phe Cys Ile Asp Tyr Ala Ala Val Pro Lys Leu

1115 1120 1125

Asn Pro Thr Tyr Thr Ala Val Asn Leu Ser Ala Asp Asp Thr Asn

1130 1135 1140

Trp Arg Lys Thr Arg Phe Val Ile Val Ala Val Glu Gly Ser Tyr

1145 1150 1155

Asp Asp Asp Asp Asp Gln Lys Gly Gly Thr Ile Lys Glu Ile Ala

1160 1165 1170

Leu Met Val Ala Pro Lys Asn

1175 1180

<210> 50

<211> 1215

<212> PRT

<213> 鼻窦假囊酵母(Eremothecium sinecauda)

<400> 50

Met Arg Asp Val Ile Thr Ser Leu Phe Leu Lys Pro Gln Gln Arg Arg

1 5 10 15

Thr Gly Ser Thr Cys Val Asp Ile Glu Ser Asp Pro Val Ser Asn Lys

20 25 30

Ala Thr Asp Phe Thr Val Gly Gln Glu Pro Val Ile Ser Glu Pro Arg

35 40 45

Lys Ser Thr Lys Thr Leu Asn Leu Met Ser Arg Met Met Ala Ala Leu

50 55 60

Thr Val Val Ile Leu Val Val Thr Leu Leu Ala Pro Phe Ser Val Gly

65 70 75 80

Thr Gly Arg Asp Met Ile Leu Leu Ser Pro Val Pro Glu Ser Arg Lys

85 90 95

Val Gly Gly Gly Lys Glu Gly Met Asp Ala Glu Leu Gln Asp Arg Phe

100 105 110

Arg Val Ser Gln His Arg Ser Thr Ser Lys Lys Leu Tyr Glu Leu Leu

115 120 125

Arg Asp Ser Glu Lys Thr Phe Tyr Asp Glu Glu Glu Gln Val Leu Gly

130 135 140

Thr Thr Val Ala Ser Arg Asn Thr Tyr Ser Arg Gln Pro Tyr Val Ala

145 150 155 160

Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Val Gly Phe Gly Tyr Ala

165 170 175

Leu Asp Glu Leu Ser Leu Trp Thr Gly Asp Ser Asp Ile Pro Gly Ala

180 185 190

Leu Asp Asn Gly Trp Pro Leu Arg Asn Gln Arg Phe Ala Gly Ala Phe

195 200 205

Val Ser Asp Phe Tyr Gly Leu Val Glu Asn Ile Asn Ser Thr Asn Phe

210 215 220

Arg Glu Leu Asp Glu Glu Gly Tyr Thr Thr Val Ile Ser Ala Ile Pro

225 230 235 240

Gln Trp Thr Asp Leu Arg Leu Ser Val Phe Lys Asp Asn Asp Asp Asn

245 250 255

Pro Tyr Ala Thr Leu Ser Pro Val Glu Leu Asp Leu Ser Gln Val Ser

260 265 270

Gln Tyr Thr Gln Asn Leu Ser Leu Gln Asp Gly Ile Val Cys Thr Glu

275 280 285

Phe Asn Trp Met Asn Leu Leu His Ile Lys Thr Ser Val Ile Ala His

290 295 300

Arg Lys Ile Phe Thr Leu Gly Leu Val Glu Met Glu Val Ser Leu Leu

305 310 315 320

Glu Asp Val Lys Leu Gly Lys Gly Gly Ser Leu His Val Glu Ile Glu

325 330 335

Asp Met Phe Asp Phe Glu Ser Ser Arg Arg Thr Phe Leu Arg Asp His

340 345 350

Gly Tyr Asp Asp Glu Gly Ile Tyr Met Val Val Glu Pro Glu Asn Val

355 360 365

Pro Tyr Ser Asn Ala Ser Leu Tyr Ser Arg Cys Asp Val Glu Gly Gly

370 375 380

Ser Glu Val Ser Asp Val Glu Arg Tyr Val Thr Asn Asn Thr Val Ile

385 390 395 400

Gln Ser His Ser Val Tyr Leu Asp His Ser Ser Pro Ile Val Val Val

405 410 415

Gln Lys Tyr Val Gly Ile Val Ser Thr Glu Tyr Asn Glu Glu Pro Ile

420 425 430

Ser Asn Leu Ala Arg Ala Lys Glu Val Val Gln Asp Cys Phe Gly Ser

435 440 445

Phe Asp Ala Leu Leu Ser Trp His Arg Glu Ala Trp Ala Asn Leu Tyr

450 455 460

Ala Asp Ala Ser Ile Glu Ile Pro Ser Asp Phe Leu Leu Glu Leu Ala

465 470 475 480

Ala Lys Ser Ser Leu Tyr His Leu Leu Ala Asn Thr Arg Ser Gln Asn

485 490 495

Val Ser Ser Ser Arg Gly Leu Pro Val Ala Val Ala Gly Leu Ser Ser

500 505 510

Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Ala Asp Thr Trp Ile Leu

515 520 525

Pro Gly Ile Leu Pro Phe Phe Pro Asp Ile Ala Lys Glu Met Ala Asn

530 535 540

Tyr Arg Asn Ala Thr His Phe Gln Ala Ile Glu Asn Ala Gln Lys Tyr

545 550 555 560

Asn Tyr Ser Gly Ala Leu Tyr Pro Trp Thr Ser Gly Arg Phe Ala Asn

565 570 575

Cys Thr Ser Thr Gly Pro Cys Val Asp Tyr Glu Tyr His Ile Asn Ile

580 585 590

Asp Ile Ala Met Ser Ser Leu Ser Ile Tyr Met Asn Gly Ala Asp Gly

595 600 605

Val Asp Asp Asp Tyr Leu Arg Tyr Thr Thr Trp Pro Leu Ile Arg Glu

610 615 620

Ala Ala Asn Phe Phe Thr Glu Tyr Val Arg Tyr Asn Glu Thr Leu Asp

625 630 635 640

Ala Tyr Thr Thr His Asn Met Thr Asp Pro Asp Glu Phe Ala Asn Phe

645 650 655

Ile Asp Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Tyr Lys

660 665 670

Trp Ala Thr Asp Val Gly Asn His Leu Glu Glu Glu Ile Asp Pro Met

675 680 685

Trp Ser Glu Ile Ser Glu Lys Ile His Ile Pro Thr Ser Pro Thr Asn

690 695 700

Ile Thr Leu Glu Tyr Thr Gly Met Asn Ala Thr Val Asp Ile Lys Gln

705 710 715 720

Ala Asp Val Met Leu Met Val Tyr Pro Leu Gly Tyr Ile Thr Asp Glu

725 730 735

Ser Ile Leu Asn Asn Ala Ile Gln Asn Leu Tyr Tyr Tyr Ser Glu Arg

740 745 750

Gln Ser Ala Ser Gly Pro Ala Met Thr Phe Pro Val Phe Val Ala Ala

755 760 765

Ala Ala Ala Leu Leu Asn His Gly Cys Ser Ser Gln Ser Tyr Leu Tyr

770 775 780

Lys Ser Val Val Pro Tyr Leu Arg Ser Pro Phe Leu Gln Phe Ser Glu

785 790 795 800

Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe

805 810 815

Pro Phe Leu Thr Ala Asn Gly Gly Tyr Leu Gln Ala Leu Leu Phe Gly

820 825 830

Leu Thr Gly Leu Arg Tyr Ser Tyr Thr Val Asn Pro Glu Thr Lys Lys

835 840 845

Met Glu Arg Leu Leu Lys Phe Asn Pro Ile Ala Leu Pro Met Leu Pro

850 855 860

Gly Gly Ile Arg Ile Asn Asn Phe Lys Tyr Met Gly Gln Val Leu Asp

865 870 875 880

Ile Ser Val Gly Asp Tyr Asn Ala Ser Ile Tyr His Lys Tyr Gly Asp

885 890 895

Val Pro Ile Asn Ile Lys Val Pro Asp Arg Ser Val Leu Arg Asp Arg

900 905 910

Glu Ile Pro Phe Pro Ser Ser Ser Asn Lys Val Gln Ala Arg Asp Met

915 920 925

Ile Asp Tyr Thr Glu Leu Lys Asp Ser Asn Tyr His Thr Val Tyr Pro

930 935 940

Gly Glu Lys Leu Val Leu Pro Val Phe Thr Pro Gly Leu Asn Ile Glu

945 950 955 960

Lys Asn Ile Ala Glu Gly Lys Gln Ile Thr Asn Leu Thr Arg Gly Val

965 970 975

Pro Gly Asp Val Ala Ile Ser Met Val Asp Gly Asn Asn Tyr Thr His

980 985 990

Trp Gln Pro Phe Asn Lys Ser Glu Arg Ala Leu Met Leu Ile Asp Leu

995 1000 1005

Gly Ser Glu Glu Asp Phe Glu Ile Thr Gly Gly Lys Ile Val Trp

1010 1015 1020

Gly Ala Arg Pro Ala Val Asn Leu Ser Leu Ser Ile Leu Pro His

1025 1030 1035

Tyr Glu Asn Met Thr Ala Val Leu Asp Ser Val Ile Glu Thr Tyr

1040 1045 1050

Gln Lys Asn Ala Ser Ser Lys Leu Thr Ser Val Arg His Lys Gly

1055 1060 1065

Ser Ser Gly Gly Val Pro Asn Gly Ser Val His Thr Ser Pro Asp

1070 1075 1080

Ile Ile Gly Ser Thr Ile Asp Met Gly Ile Arg Glu Ile Phe Asn

1085 1090 1095

Trp Asn Leu Ile Asp Phe Asp Glu Ile Ser Ala Ile Ala Pro Glu

1100 1105 1110

Val Leu Pro Lys Phe Arg Asn Phe Val Thr Ile Leu Asp Asp His

1115 1120 1125

Pro Val Glu Pro Thr Gln Pro Phe Phe His Asp Val Phe Asn Gln

1130 1135 1140

Ser Gln Ile Val Ile Leu Pro Ser Asn Glu Thr Asp Phe Tyr Ile

1145 1150 1155

Asp Tyr Asp Lys Val Ala Glu Leu Asn Pro Gly Tyr Thr Ala Ile

1160 1165 1170

Asp Phe Lys Lys Asn Asp Asn Thr Trp Arg Lys Thr Arg Phe Val

1175 1180 1185

Met Val Thr Val Glu Gly Val Tyr Asp Glu Asp Asp Asp Pro Tyr

1190 1195 1200

Gly Ala Thr Ile Lys Glu Ile Ala Leu Met Ser Thr

1205 1210 1215

<210> 51

<211> 1133

<212> PRT

<213> 米拉氏拉茜斯酵母(Lachancea mirantina)

<400> 51

Met Lys Thr Glu Lys Ala Lys Ser Arg Leu Phe Tyr Ser Val Leu Thr

1 5 10 15

Cys Leu Leu Val Leu Gly Trp Phe Thr Val Thr Ser Tyr Leu Gln Lys

20 25 30

Leu Lys Pro Gly Leu Ser Val Phe Glu Ser Thr Arg Arg Phe Ser Glu

35 40 45

Arg Val Arg Asn Arg Arg Lys Ser Ser Gln Gln Leu Tyr Glu Gln Leu

50 55 60

Ser Asp Ser Asp Asp Thr Tyr Tyr Asp Glu Val His Trp Cys Val Gly

65 70 75 80

Thr Ser Tyr Phe Ser Glu Asn Thr Phe Ser Arg Gln Pro Tyr Val Ser

85 90 95

Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Val Gly Leu Gly Tyr Ala

100 105 110

Leu Asp Thr Leu Asn Ile Trp Thr Ala Asn Ser Ser Val Pro Gly Ala

115 120 125

Leu Asp Asn Gly Trp Pro Leu Arg Asn Gln Arg Phe Ala Gly Ala Phe

130 135 140

Val Ser Asp Phe Tyr Cys Leu Glu Pro Lys Leu Asn Ser Thr Asn Phe

145 150 155 160

Pro Glu Leu Asp Asp Asp Gly Tyr Ser Thr Val Leu Ser Ala Ile Pro

165 170 175

Gln Trp Thr Asp Leu Gln Phe Ser Val Arg Asn Gly Ser Leu Lys Phe

180 185 190

Ser Ala Gln Asp Val Arg Leu Glu Asp Val Thr Asn Tyr His Gln Asn

195 200 205

Leu Ser Met Gln Thr Gly Val Val Ser Thr Glu Leu Asp Trp Leu Gly

210 215 220

Phe Leu His Val Lys Ser Ser Val Leu Ala His Arg Ser Lys His Ser

225 230 235 240

Leu Gly Ile Val Thr Leu Glu Leu Asn Val Asn Pro Gly Trp Asp Val

245 250 255

Asp Glu Val Asp Leu Glu Leu Cys Asp Cys Leu Asn Tyr Thr Thr Ala

260 265 270

Arg Arg Val Ala Leu Gln Asp Ile Gly His Asp Ser Gly Gly Ile Tyr

275 280 285

Met Thr Val Asn Pro Asp Asn Val Pro Tyr Ser Ser Ala Ser Ile Tyr

290 295 300

Ser Ser Phe Glu Leu Leu Tyr Asp Gly Glu Arg Ser Gly Lys Ser Glu

305 310 315 320

Phe Arg Phe Asp Asp Asn Arg Thr Thr Ile Ser Gln Cys Val Ser Val

325 330 335

Lys Leu Glu Ala Asp Gly Pro Ile Leu Lys Ala Asn Lys Phe Thr Gly

340 345 350

Ile Met Ser Thr Glu Tyr Gly Asp Gly Arg Arg Asp Gln Thr Asn Leu

355 360 365

Glu Phe Ala Arg Ser Val Val Lys Glu Ser Leu Gly Ser Cys His Lys

370 375 380

Leu Thr Glu Ser Asn Arg Ala Ala Trp Glu Ala Leu Tyr Ser Asp Ala

385 390 395 400

His Ile Glu Ile Pro Ser Asp Ser Leu Leu Glu Leu Ala Ala Arg Ser

405 410 415

Ser Leu Tyr His Leu Leu Ala Asn Thr Arg Arg Phe Asn Val Ser Ala

420 425 430

Glu Arg Gly Leu Pro Met Pro Val Ser Gly Leu Ser Ser Asp Ser Tyr

435 440 445

Gly Gly Met Val Phe Trp Asp Ala Asp Ile Trp Met Met Pro Ser Leu

450 455 460

Leu Pro Phe Phe Pro Glu Ile Ala Arg His Ile Val Asn Tyr Arg Asn

465 470 475 480

Gly Thr His Gln Gln Ala Val Leu Asn Ala Gln Lys Tyr Asp Tyr Ser

485 490 495

Gly Ala Leu Tyr Pro Trp Thr Ser Gly Arg Phe Ala Asn Cys Thr Ser

500 505 510

Thr Gly Pro Cys Val Asp Tyr Glu Tyr His Val Asn Phe Asp Ile Ala

515 520 525

Met Ala Ser Phe Leu Met Tyr Leu Asn Gly Glu Gly Glu Asp Tyr Leu

530 535 540

Arg Tyr Thr Thr Trp Pro Leu Val Arg Asp Ala Ala Lys Phe Phe Ala

545 550 555 560

Glu Tyr Val Gln Ile Asn His Thr Thr Gly Gln Tyr Glu Thr Phe Asn

565 570 575

Leu Thr Asp Pro Asp Glu Tyr Ala Asn Phe Val Asn Asn Gly Ala Phe

580 585 590

Thr Asn Ala Gly Ile Lys Thr Leu Met Lys Trp Ala Thr Asp Ile Gly

595 600 605

Tyr His Leu Gly Asn Glu Val Asp Pro Lys Trp Asn His Ile Ser Lys

610 615 620

Asn Ile Tyr Ile Pro Met Ala Glu Ser Asn Ile Thr Leu Glu Tyr Ser

625 630 635 640

Gly Met Asn Ala Ser Val Glu Ile Lys Gln Ala Asp Val Val Leu Met

645 650 655

Thr Tyr Pro Leu Gly Tyr Ile Thr Asp Glu Thr Ile Leu Lys Asn Ala

660 665 670

Ile Arg Asn Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro

675 680 685

Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ser Ser Leu Leu Asn His

690 695 700

Gly Cys Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Leu Pro Tyr Leu

705 710 715 720

Arg Ala Pro Phe Met Gln Phe Ser Glu Gln Ser Asp Asp Asp Phe Leu

725 730 735

Thr Asn Gly Leu Thr Gln Pro Ser Phe Pro Phe Leu Thr Gly Asn Ala

740 745 750

Gly Phe Leu Gln Gly Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser

755 760 765

Tyr Glu Val Asp Pro Ala Ser Lys Thr Ile Asn Arg Phe Leu Lys Phe

770 775 780

Ile Pro Met Lys Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn

785 790 795 800

Phe Lys Tyr Met Gly Gln Val Leu Asp Val Ile Ile Asp Asp Lys Asn

805 810 815

Ala Thr Leu Ile His Lys Gln Gly Asp Leu Ala Val Thr Leu Lys Thr

820 825 830

Phe Asn Arg Ser Phe Asn Thr Asp Arg Gln Leu Gly Ala His His Met

835 840 845

Trp Thr Glu Leu Leu Ala Lys Asn Glu Ser Leu Pro Ala Thr Phe Tyr

850 855 860

Thr Leu Asn Pro Arg Glu Thr Leu Ser Leu Pro Leu Phe Glu Pro Leu

865 870 875 880

Leu Asn Ile Asp Asn Asn Leu Ala Glu Gly Lys Gln Ile Thr Asn Phe

885 890 895

Thr Ala Gly Val Pro Gly Asp Val Val Tyr Ser Ala Val Asp Gly Asn

900 905 910

Asn Tyr Thr His Trp Gln Pro Leu Asn Lys Asp Met Arg Ala Lys Leu

915 920 925

Leu Ile Asp Leu Gly Pro Asp Asn Asp Lys Pro Leu Thr Arg Gly Met

930 935 940

Ile Leu Trp Gly Lys Arg Pro Ala Arg Asn Val Ser Val Ser Val Leu

945 950 955 960

Pro Ser His Gly Lys Tyr Val Lys Gly Ile Lys Ile Lys Lys Arg Glu

965 970 975

Glu Asp Glu Thr Gly Phe Cys Pro Leu Thr Ile Leu Arg Gly Arg Cys

980 985 990

Asn Val Ala Ser Ala Ile Ala Leu Pro Glu Asp Ser His Ile Phe Asp

995 1000 1005

Thr Glu Leu Arg Asp Met Leu Asn Ser Glu Arg Leu Asp Leu Glu

1010 1015 1020

Gln Leu Thr Arg Leu Ser Pro Asn Tyr Ser Val Phe Thr Asp Arg

1025 1030 1035

Phe Val Thr Ile Leu Glu Asp Phe Gln Val Phe Pro Ser Glu Pro

1040 1045 1050

Tyr Tyr Glu Thr Leu Gln Arg Asn Lys Ser Ile Val Ile Leu Pro

1055 1060 1065

Ser Asn Lys Thr Glu Phe Val Phe Asp Tyr Glu Glu Leu Cys Met

1070 1075 1080

Arg Ala Cys Asn Asp Thr His Val Cys Ala Glu Asn Ala Ile Thr

1085 1090 1095

Gly Leu Pro Pro Pro Arg Phe Val Leu Leu Ser Val Glu Gly Thr

1100 1105 1110

Tyr Asp Asp Asp Lys Asp Pro Lys Gly Ala Thr Ile Glu Glu Ile

1115 1120 1125

Ala Phe Leu Ser Asp

1130

<210> 52

<211> 1070

<212> PRT

<213> 近平滑假丝酵母(candida orthopsilosis)

<400> 52

Met Asp Ile Glu Asn Asn Gly Ile Tyr Ile Glu Asp Ser Ser Asn Thr

1 5 10 15

Glu Gly Ile Lys Lys Ser Leu Arg Asp Asn Lys Val Tyr Ile Val Ser

20 25 30

Met Thr Leu Val Ala Asn Ile Ile Leu Phe Tyr Ser Leu His Leu Leu

35 40 45

Asn Ala Arg Phe Cys Glu Ala Phe Pro Phe Val Leu Pro Arg Ser Glu

50 55 60

Lys Tyr Thr Leu Gln Gln Ser Asp Ser Asn Ile Gly Asp Phe Val Asn

65 70 75 80

Ser Phe Ala Asn Lys Glu Ile Phe Ser Gln Ile Gln Phe Ser Glu His

85 90 95

Ala Phe Tyr Asp Arg Glu Leu Asn Val Val Gly Thr Ile Glu Phe Ser

100 105 110

Lys Tyr Asn Gln Tyr Gln Lys Gln Pro Tyr Val Ala Asn Gly Tyr Ile

115 120 125

Gly Ser Arg Ile Pro Asn Leu Gly Gln Gly Phe Thr Tyr Asp Gln Leu

130 135 140

Thr Asn Ser Pro Asp Ser Lys Lys Asp Asp Leu Tyr Asn Gly Trp Pro

145 150 155 160

Leu Phe Asp Lys Arg Phe Ser Gly Ala Phe Ile Ala Gly Phe Tyr Asp

165 170 175

Leu Gln Glu Asn Thr Thr Gly Asn Asn Phe Pro Glu Leu Leu Lys Asn

180 185 190

Gly Tyr Glu Ser Val Ile Ser Ala Val Pro Gln Trp Thr Thr Leu Lys

195 200 205

Leu Val Ile His Arg Asp Ser Lys Asn Tyr Thr Leu Asp Pro Ser Ser

210 215 220

Pro Asp Gly Ile Gly Glu Ile Thr Asn Tyr Val Gln Asn Met Ser Leu

225 230 235 240

Ser Thr Gly Ile Val Thr Thr Gln Phe Thr Trp Leu Asp Leu Leu Asp

245 250 255

Val Lys Tyr Thr Val Leu Ala His Arg Ser Glu Ile Asn Leu Gly Leu

260 265 270

Val Lys Val Asp Ile Gln Asn Lys Gly Asn Asp Thr Ile Glu Leu Asp

275 280 285

Val Ile Asp Glu Leu Asn Phe Asp Thr Ala Gln Arg Cys Glu Leu Asn

290 295 300

Ser Ile Gly His Asp Asn Ser Gly Ile Tyr Val Ser Phe Ser Pro Leu

305 310 315 320

Asn Leu Asp Tyr Ile Ser Gly Ala Ile Tyr Ser Thr Leu His Leu Asp

325 330 335

Asp Ile Thr Arg Arg Ser Asn Asn Lys Gln Thr Lys Gln Thr Ser Ser

340 345 350

Ile Arg Leu Asn Ala Gln Asp Ser Thr Gln Phe Gly Lys Thr Val Gly

355 360 365

Ile Ala Ser Thr Asp Leu Asn Pro Ser Lys Phe Glu Asn Thr Arg Asp

370 375 380

Thr Leu Gln Phe Ala Lys Lys Val Ser Gln Lys Phe Glu Tyr Leu Asp

385 390 395 400

Leu Ile Ile Lys Ser His Val Gln Ala Trp Lys Glu Val Thr Gly Lys

405 410 415

Ala Pro Ser Leu Ser Phe Pro Asn Asp Pro Leu Leu Asp Leu Gly Ser

420 425 430

Lys Ala Ser Val Tyr His Leu Leu Ala Asn Thr Arg Ala Asp Ala Gln

435 440 445

Gly Leu Thr Gly Ala Leu Gly Val Ala Gly Leu Ser Ser Asp Ser Tyr

450 455 460

Gly Gly Met Val Phe Trp Asp Thr Asp Phe Trp Met Phe Lys Ala Ile

465 470 475 480

Leu Ala Leu Asn Pro Ser His Ala Lys Ser Ile Val Asn Tyr Arg Val

485 490 495

His Thr His Glu Gln Ala Leu Lys Asn Thr Pro Ser Gly His Glu Gly

500 505 510

Ala Ala Tyr Pro Trp Thr Ser Gly Arg Phe Gly Asn Cys Thr Ala Thr

515 520 525

Gly Pro Cys Leu Asn Tyr Glu Tyr His Ile Asn Ser Ala Ile Ala Met

530 535 540

Ala Ala Trp Glu Leu Tyr Leu Ser Gly Ala Val Asp Asp Gln Tyr Leu

545 550 555 560

Glu Ser Thr Val Tyr Pro Leu Ile Asn Asp Ala Ala Lys Phe Tyr Ala

565 570 575

Asp Tyr Val Thr Ser Tyr Asn Asp Thr Ile Asp Lys Phe Val Thr Asn

580 585 590

Asn Leu Thr Asp Pro Asp Glu Tyr Ala Asn His Val Asp Asn Gly Ala

595 600 605

Tyr Thr Asn Ala Gly Ile Ala Leu Leu Met Lys Trp Ile Asn Asp Ile

610 615 620

Gly Asp Val Leu Asn Val Lys Met Pro Glu Ile Tyr Thr Glu Ile Ser

625 630 635 640

Gln Lys Met Tyr Leu Pro Thr Ala Asn Asn Ser Gln Asn Ile Thr Leu

645 650 655

Glu Tyr Ser Gly Met Asn Ser Ser Val Gly Ile Lys Gln Ala Asp Val

660 665 670

Val Leu Leu Thr Tyr Pro Leu Gln Asn Ser Leu Val Asp Asp Glu Gln

675 680 685

Ala Tyr Thr Asn Met Glu Phe Tyr Ser Gly Lys Gln Val Ser Tyr Gly

690 695 700

Pro Ala Met Thr Phe Ser Ile Phe Ser Ile Val Ala Ala Asn Leu Ala

705 710 715 720

Ser Ser Gly Cys Ala Ser Gln Ser Tyr Leu His Lys Ala Met Gln Pro

725 730 735

Tyr Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Asn Asn Asp Asp

740 745 750

Tyr Lys Ser Asn Gly Gly Thr His Pro Ala Phe Pro Phe Leu Thr Ala

755 760 765

His Gly Gly Phe Val Gln Ala Val Ile His Gly Leu Ala Gly Leu Arg

770 775 780

His Ser Tyr Ala Ile Asp Asp Gly Lys Ile Ser Arg Ser Leu Phe Leu

785 790 795 800

Asp Pro Ile Ala Leu Pro Cys Leu Gly Asn Gly Val Gln Tyr Ser Gly

805 810 815

Ile His Tyr Asp Asn His Thr Ile Ser Met Asn Ile Thr Glu Ser Thr

820 825 830

Phe Thr Ile Lys Asn Glu Gly Lys Thr Asn Asn Arg Ala Asn Asp Tyr

835 840 845

Ile Ser Ile Thr Ile Ala Asp Arg Asn Pro Gly Arg Gly Thr Tyr Lys

850 855 860

Leu Asn Asp Asn Glu Glu Lys Ser Phe Lys Leu Tyr Gln Pro Asn Lys

865 870 875 880

Asn Phe Glu Asp Ser Ile Ser Glu Cys Gly Leu Ala Thr Phe Tyr Asn

885 890 895

Ile Thr Glu Ser Ala Pro Gly Asp Ser Ser Phe Ser Ile Asn Asp Gly

900 905 910

Asp Asn Thr Thr Arg Trp Gln Ala Lys Tyr Asn Asp Thr Thr Gly Lys

915 920 925

Val Leu Ile Asp Leu His Ser Ile Lys Asn Ile Thr Gly Val Ile Phe

930 935 940

Asn Trp Ala Asp Lys Pro Pro Lys Lys Val Thr Leu Ser Lys Tyr Ser

945 950 955 960

Asp Thr Lys Phe Lys Ala Val Thr Asp Phe Leu Ala Lys Val Asp Phe

965 970 975

Gly Asn Pro Val Tyr Lys Asn Tyr Arg Phe Ala Asn Pro Asp Glu Lys

980 985 990

Leu Leu Asn Gln Ser Asp Ala Phe Glu Glu Val His Ser Glu Asp Val

995 1000 1005

Leu Ile Ser Ala Pro Phe Ser Asn Lys Glu Phe Glu Lys Val Leu

1010 1015 1020

Val Pro Val Arg His Asn Thr Thr Ser Trp Asp Ile Asn Leu Lys

1025 1030 1035

Thr Arg Phe Leu Leu Leu Glu Val Asp Ser Ile His Asn Thr Val

1040 1045 1050

Pro Ile Glu Asp Asp Tyr Gly Gly Ala Lys Ile Ala Glu Val Ser

1055 1060 1065

Phe Phe

1070

<210> 53

<211> 1044

<212> PRT

<213> 麦芽糖假丝酵母(Candida maltosa)

<400> 53

Met Ile Phe Phe Ala Asn Leu Phe Val Leu Phe Ser Leu His Leu Leu

1 5 10 15

Tyr Ala Lys Phe Cys Glu Gly Tyr Pro Asn Val Val Pro Val Lys Ile

20 25 30

Arg Ser Thr Gly Arg Ile His Arg Pro Asn Leu Glu Lys Leu Val Asn

35 40 45

Ser Pro Glu Asn Lys Gln Ile Phe Ser Gln Leu Lys Phe Ser Asp His

50 55 60

Ala Phe Tyr Asp Thr Thr Ala Asn Thr Val Gly Thr Ile Glu Phe Pro

65 70 75 80

Val Phe Asn Gln Tyr Gln Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile

85 90 95

Gly Ser Arg Ile Pro Asn Leu Gly His Gly Phe Thr Tyr Asp Gln Leu

100 105 110

Thr Asn Ser Ser Asp Ala Asn Asp Asp Asp Leu Val Asn Gly Trp Pro

115 120 125

Leu Phe Asn Lys Arg Tyr Ala Gly Ala Phe Ile Ala Gly Phe Phe Asp

130 135 140

Ile Gln Lys Asn Thr Thr Gly Thr Asn Phe Pro Glu Leu Leu Glu Asn

145 150 155 160

Gly Tyr Glu Ser Val Ile Ala Ala Val Pro Gln Trp Thr Ser Leu Ser

165 170 175

Leu Ser Val Thr Ile Gly Gly Lys Asn Tyr Thr Leu Asp Pro Ala Leu

180 185 190

Glu Pro Glu Leu Ile Gly Asn Ile Thr Asn Tyr Val Gln Asn Leu Ser

195 200 205

Met Ser Asp Gly Ile Val Thr Thr Gln Phe Thr Trp Leu Asn Ser Ile

210 215 220

Asp Val Lys Tyr Glu Val Leu Ala His Arg Thr Glu Ile Asn Leu Gly

225 230 235 240

Leu Val Asn Leu Gln Ile Ser Asn Arg Gly Gln Gln Ser Ile Asp Val

245 250 255

Ile Val Thr Asp Glu Leu Asp Phe Glu Thr Ser Lys Arg Cys Gln Leu

260 265 270

Asn Lys Ala Ala Phe Asp Asp Gln Gly Ile Tyr Val Thr Phe Ser Pro

275 280 285

His Asp Leu Asp Tyr Val His Gly Ala Ile Tyr Ser Thr Leu Ile Ser

290 295 300

Ser Asp Ala Ser Pro Cys Lys Ser Ser Asn Glu Thr Val Thr Ser Gln

305 310 315 320

Ser Leu Gln Leu Ser Leu Glu Cys Asn Ala Asn Phe Glu Val Thr Lys

325 330 335

Val Val Gly Val Val Ser Ser Asp Leu Asp Pro Asn Ser Phe Lys Ser

340 345 350

Ser Asp Asp Val Leu Gln Tyr Ala Lys Lys Ile Ser Gln Lys Tyr Asn

355 360 365

Asn Val Asp Gln Ile Ile Asn Ser His Lys Glu Gly Trp Ser Glu Ile

370 375 380

Leu Gln His Ser Pro Leu Ile Thr Phe Pro Ser Asp Pro Leu Leu Asn

385 390 395 400

Leu Gly Ala Arg Ala Ser Ile Phe His Leu Leu Ala Asn Thr Arg Pro

405 410 415

Asp Ala Gln Gly Val Thr Gly Ala Leu Gly Val Gly Gly Leu Ser Ser

420 425 430

Asp Ser Tyr Ala Gly Met Val Phe Trp Asp Ala Asp Leu Trp Met Leu

435 440 445

Asn Gly Ile Leu Pro Phe Ala Pro Ala His Ala Lys Ser Leu Val Asn

450 455 460

Tyr Arg Leu His Thr His Asn Gln Ala Ile Glu Asn Val Pro Glu Gly

465 470 475 480

Tyr Asn Gly Ala Val Tyr Pro Trp Thr Ser Gly Arg Phe Gly Asn Cys

485 490 495

Thr Ala Thr Gly Pro Cys Leu Asp Tyr Glu Tyr His Ile Asn Met Ala

500 505 510

Val Ser Met Ala Ala Trp Gln Leu Tyr Ile Ser Gly Ala Ala Asp Asp

515 520 525

Asp Phe Leu Ala Asp Val Ala Tyr Pro Leu Ile Asn Asp Ala Ala Thr

530 535 540

Phe Phe Ser Glu Tyr Val Thr His Phe Asn Asn Thr Val Asp Lys Tyr

545 550 555 560

Val Thr Lys Asn Leu Thr Asp Pro Asp Glu Tyr Ala Asn His Val Asp

565 570 575

Asn Gly Ala Tyr Thr Asn Ala Gly Ile Ala Leu Val Met Lys Trp Ala

580 585 590

Gln Ile Val Gly Asn His Leu Gly Lys Asp Val Pro Asn Thr Tyr Lys

595 600 605

Asp Ile Thr Glu Lys Met Phe Leu Pro Thr Gly Asp Asn Ser Gln Asn

610 615 620

Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Gly Ile Lys Gln

625 630 635 640

Ala Asp Val Ile Met Ile Thr Tyr Pro Leu Glu Asn Glu Leu Ile Asp

645 650 655

Glu Asp Gln Ala Tyr Thr Asn Met Glu Phe Tyr Ser Met Lys Gln Val

660 665 670

Ser Tyr Gly Pro Ala Met Thr Phe Pro Ile Phe Ser Ile Val Ala Ala

675 680 685

Asn Leu Ala Pro Thr Gly Cys Ala Ser Gln Ser Tyr Leu Gln Lys Ala

690 695 700

Ile Gln Pro Phe Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Asn

705 710 715 720

Asn Asp Asn Phe Leu Thr Asn Gly Gly Thr His Pro Ala Phe Pro Phe

725 730 735

Leu Thr Ala His Gly Gly Phe Leu Gln Ala Thr Leu Gln Gly Leu Thr

740 745 750

Gly Met Arg Tyr Gly Phe Thr Val Asp Glu Gln Ala Ser Lys Leu Val

755 760 765

Arg Val Leu Asn Leu Asp Pro Ile Ala Leu Pro Cys Leu Gly Asp Gly

770 775 780

Val Gln Phe Asp Gly Ile Lys Tyr Asp Asn His Thr Ile Ser Met Ser

785 790 795 800

Ile Asn Gln Thr His Phe Thr Ile Lys Asn Thr Gly Lys Thr Asp Ser

805 810 815

Asn Gly Gly Asn Ser Phe Ile Thr Ile Ser Leu Ala Glu Arg Asn Pro

820 825 830

Asp His Gly Gln Tyr Thr Ile Asn Asp Asn Glu Glu Lys Ser Phe Ala

835 840 845

Leu Phe Lys Pro Asp Thr Ser Phe Ile Asp Ser Ile Ser Glu Cys Gly

850 855 860

Gln Ala Asp Phe Tyr Asn Ile Thr Glu Ser Ala Phe Gly Asp Ser Pro

865 870 875 880

Ile Ser Ile Asn Asp Gly Asp Asn Thr Thr Arg Trp Gln Val Lys Tyr

885 890 895

Asn Asp Thr Val Gly Lys Ile Leu Val Asp Phe Lys Ser Phe Lys Asn

900 905 910

Ile Ser Gly Val Thr Phe Asn Trp Ala Asp Lys Pro Pro Lys Asn Leu

915 920 925

Lys Leu Ser Lys Tyr Ser Gly Asp Gln Phe Thr Thr Val Thr Asp Phe

930 935 940

Phe Ala Lys Val Asp Phe Gly Asn Glu Leu Tyr Asp Ser Tyr Lys Tyr

945 950 955 960

Ala Asn Pro Glu Asp Lys Ile Tyr Asn Gln Ser Glu Val Phe Gln Glu

965 970 975

Ile His Ser Asp Ser Val Glu Ile Ser Ala Pro Phe Asp Asn Asp Glu

980 985 990

Phe Ser Gln Val Leu Val Pro Thr Arg His Asn Thr Thr Ile Leu Asp

995 1000 1005

Leu Glu Leu Ser Gly Arg Phe Leu Leu Ile Glu Val Asp Ala Ile

1010 1015 1020

His Asn Thr Val Pro Ile Asp Gly Asp Phe Gly Gly Ala Lys Leu

1025 1030 1035

Ala Glu Val Val Phe Tyr

1040

<210> 54

<211> 1083

<212> PRT

<213> 热带假丝酵母(Candida tropicalis)

<400> 54

Met Gly Arg Lys Pro Ser Ser Ile Ile Pro Glu Glu Glu Val Asp Glu

1 5 10 15

Asp Glu Glu Asp Ser Lys Leu Leu Phe Leu Leu Asn Phe Leu Lys Ser

20 25 30

Leu Pro Ile Lys Lys Ile Trp Ile Val Met Ile Val Phe Ala Asn Leu

35 40 45

Phe Val Phe Ile Ser Phe Gln Leu Phe Tyr Ala Lys Phe Ala His Gly

50 55 60

Phe Pro Gly Val Ile Pro Leu Lys Ala Arg Ser Pro Glu Gly Leu His

65 70 75 80

Thr Val Asp Leu Ser Lys Leu Ile Asp Ser Gln Glu Asn Lys Lys Ile

85 90 95

Phe Ser Gln Met Lys Phe Ser Asp Gln Ala Phe Tyr Asp Thr Thr Asp

100 105 110

Asn Val Val Gly Thr Thr Glu Phe Thr Glu Phe Asn Gln Tyr Gln Arg

115 120 125

Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Ile

130 135 140

Gly Gln Gly Phe Ala Tyr Asp Gln Leu Ser Asn Ser Ser Glu Ala Asn

145 150 155 160

Glu Asp Asp Leu Ser Asn Gly Trp Pro Leu Phe Asn Lys Arg Tyr Ala

165 170 175

Gly Ala Phe Ile Ala Gly Phe Tyr Asp Ile Gln Lys Asn Thr Thr Gly

180 185 190

Thr Asn Phe Pro Glu Leu Leu Glu Asn Gly Tyr Glu Ser Val Ile Ala

195 200 205

Ala Val Pro Gln Trp Thr Ser Leu Ser Leu Ser Val Glu Ile Asp Gly

210 215 220

Gln Val Tyr Ile Leu Asp Pro Ala Leu Asp Ser Gln Asp Met Gly Gly

225 230 235 240

Ile Ser Asn Tyr Val Gln Asn Leu Ser Leu Ser Asp Gly Ile Val Ser

245 250 255

Thr Gln Phe Thr Trp Leu Asn Lys Leu Asn Val Lys Tyr Glu Val Leu

260 265 270

Ala His Arg Thr Asn Ile Asn Leu Gly Leu Val Asp Leu Gln Ile Ser

275 280 285

Asn Ile Gly Asn Glu Asn Leu Glu Leu Thr Val Thr Asp Ser Leu Asp

290 295 300

Phe Asn Thr Ser Gln Arg Cys Gln Leu Asn Asp Val Ser Thr Asp Asp

305 310 315 320

Gln Gly Ile Ser Ile Thr Phe Ser Pro His Asp Leu Asp Tyr Ile His

325 330 335

Gly Ala Ile Tyr Ser Lys Leu Val Leu Asp Gly Glu Ile Thr Arg Glu

340 345 350

Val Gln Thr Tyr Glu Lys Val Thr Gln Ser Ser Lys Ile Thr Ile Gly

355 360 365

Cys Asn Ser Asn Ile Lys Ile Ser Lys Phe Val Gly Val Ala Ser Ser

370 375 380

Asp Leu Gln Pro Asp Gln Leu Lys Thr Ser Asp Asp Val Leu Gln Leu

385 390 395 400

Ala Lys Ser Val Ala Gln Glu Cys His Asn Ala Glu Gln Ile Ile Asp

405 410 415

Ser His Lys Gln Ala Trp Ser Glu Val Ile Asn Asn Gly Pro Leu Val

420 425 430

Thr Phe Pro Ser Asp Pro Leu Leu Asn Leu Gly Ala Arg Ala Ser Val

435 440 445

Phe His Leu Leu Ala Asn Thr Arg Pro Asp Ala Gln Gly Val Thr Gly

450 455 460

Ala Leu Gly Val Gly Gly Leu Ser Ser Asp Ser Tyr Ala Gly Met Val

465 470 475 480

Phe Trp Asp Ala Asp Leu Trp Met Leu Asn Gly Ile Leu Pro Phe Ala

485 490 495

Pro Ser His Ala Lys Ser Leu Ile Asn Tyr Arg Leu His Thr Arg Gln

500 505 510

Gln Ala Ile Glu Asn Val Pro Glu Gly Tyr Gln Gly Ala Val Tyr Pro

515 520 525

Trp Thr Ser Gly Arg Phe Gly Asn Cys Thr Ala Thr Gly Pro Cys Leu

530 535 540

Asp Tyr Glu Tyr His Ile Asn Met Ala Val Ala Met Ala Ala Trp Gln

545 550 555 560

Leu Tyr Ile Ser Gly Ala Ala Asp Asp Leu Tyr Leu Glu Thr Ile Ala

565 570 575

Tyr Pro Ile Ile Tyr Asp Ala Ala Asn Phe Phe Ser Asp Tyr Val Val

580 585 590

Asn Phe Asn Asp Ser Leu Asn Lys Tyr Thr Thr Ser Asn Leu Thr Asp

595 600 605

Pro Asp Glu Tyr Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn Ala

610 615 620

Gly Ile Ala Leu Val Met Arg Trp Ala Gln Val Ile Gly Gly His Leu

625 630 635 640

Gly Lys Val Val Pro Gln Leu Phe Gln Asp Ile Glu Gln Asn Ile Phe

645 650 655

Leu Pro Thr Ala Asn Asn Ser Gln Asn Ile Thr Leu Glu Tyr Ser Gly

660 665 670

Met Asn Ser Ser Val Gly Ile Lys Gln Ala Asp Val Ile Met Met Thr

675 680 685

Tyr Pro Leu Glu Asn Glu Leu Ile Asp Asp Glu Gln Ala Tyr Ile Asn

690 695 700

Met Glu Phe Tyr Ser Met Lys Gln Val Ser Tyr Gly Pro Ala Met Thr

705 710 715 720

Phe Pro Ile Phe Ser Val Val Ala Ala Asn Leu Ala Pro Thr Gly Cys

725 730 735

Ala Ser Gln Ser Tyr Leu Gln Lys Ala Ile Gln Pro Phe Leu Arg Gly

740 745 750

Pro Phe Ala Gln Phe Ser Glu Gln Asn Asn Asp Asn Tyr Leu Thr Asn

755 760 765

Gly Gly Thr His Pro Ala Phe Pro Phe Leu Thr Ala His Gly Gly Phe

770 775 780

Leu Gln Ala Thr Leu Gln Gly Leu Thr Gly Leu Arg Tyr Asp Tyr Asp

785 790 795 800

Ile Asp Glu Asn Gly Lys Leu Val Arg Ile Leu Arg Leu Asp Pro Ile

805 810 815

Gly Leu Pro Cys Leu Glu Glu Gly Val Ser Phe Asn Gly Ile Gln Tyr

820 825 830

Asp Asn His Thr Ile Ser Met Ser Ile Asn Gln Thr His Phe Thr Ile

835 840 845

Thr Asn Lys Cys Lys Thr Asn Pro Asn Ala Asn Asp His Ile Thr Ile

850 855 860

Leu Leu Ala Glu Arg Asn Pro Asp Asn Gly Gln Tyr Val Ile Asn Asp

865 870 875 880

Gly Glu Thr Lys Ser Phe Ala Leu Phe Lys Pro Asp Ile Ser Phe Pro

885 890 895

Asp Ser Ile Ser Glu Cys Gly Gln Ala Ser Phe Tyr Asn Val Thr Glu

900 905 910

Gly Ala Phe Gly Asp Ala Ser Ile Ser Ile Asn Asp Gly Asp Asn Thr

915 920 925

Thr Arg Trp Gln Val Lys Tyr Asn Asp Thr Val Gly Lys Val Ile Val

930 935 940

Asp Phe Lys Ser Phe Lys Asn Ile Ser Gly Val Thr Phe Asn Trp Ala

945 950 955 960

Asp Lys Pro Pro Ile Asn Val Lys Leu Ser Lys Tyr Ser Gly Asp Gln

965 970 975

Phe Thr Ser Ile Val Asp Phe Phe Ala Lys Val Asp Phe Gly Asn Glu

980 985 990

Leu Tyr Asn Ser Tyr Lys Tyr Ala Asn Pro Glu Asp His Ile Phe Asn

995 1000 1005

Gln Ser Glu Val Phe Glu Glu Ile His Ser Glu Ser Val Thr Ile

1010 1015 1020

Ser Ala Pro Phe Asp Asn Lys Glu Tyr Thr Gln Val Leu Val Pro

1025 1030 1035

Thr Arg His Asn Thr Thr Ser Ile Asp Leu Asn Leu Ser Thr Arg

1040 1045 1050

Phe Leu Leu Ile Glu Val Asp Asn Ile His Asn Thr Val Pro Ile

1055 1060 1065

Asp Asp Asp Tyr Gly Gly Ala Lys Leu Ala Glu Val Val Phe Tyr

1070 1075 1080

<210> 55

<211> 1078

<212> PRT

<213> 白假丝酵母(Candida albicans)

<400> 55

Met Ala Ala Asn Ser Ser Phe Phe Leu Ala Asp Asn Cys Ala Pro His

1 5 10 15

Asn Gln Ser Phe Ile Gln Phe Cys Ile His Ala Ala Ser Lys Lys Lys

20 25 30

Gly Trp Ile Ala Leu Met Cys Leu Ala Asn Leu Phe Leu Leu Phe Ser

35 40 45

Phe His Leu Leu Tyr Ala Arg Phe Cys Ser Gly Phe Pro Tyr Val Val

50 55 60

Pro Ser Thr Ala Arg Ser Thr Asn Gln Ile Phe His Thr Asn Leu Glu

65 70 75 80

Gln Leu Val Asn Ser Pro Glu Asn Lys Gln Ile Phe Ser Gln Leu Lys

85 90 95

Phe Ser Asp Gln Ala Phe Tyr Asp Pro His Asp Asn Val Val Gly Thr

100 105 110

Thr Glu Phe Pro Val Phe Asn Gln Tyr Gln Arg Gln Pro Tyr Val Ala

115 120 125

Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Leu Gly Gln Gly Phe Thr

130 135 140

Tyr Asp Gln Leu Thr Asn Ser Ser Thr Ala Asn Asp Asp Asp Leu Leu

145 150 155 160

Asn Gly Trp Pro Leu Phe Asn Lys Arg Tyr Ser Gly Ala Phe Val Ala

165 170 175

Gly Phe Tyr Asp Leu Gln Lys Asn Thr Thr Gly Thr Asn Phe Ala Glu

180 185 190

Leu Leu Glu Asn Gly Tyr Glu Ser Val Ile Ala Ala Val Pro Gln Trp

195 200 205

Thr Ala Leu Ser Leu Ser Val Glu Ile Leu Gly Lys Lys Tyr Thr Leu

210 215 220

Asp Pro Ser Leu Glu His Glu Ala Ile Gly Asp Ile Thr Asn Tyr Val

225 230 235 240

Gln Asn Met Ser Leu Ser Asp Gly Ile Val Thr Thr Gln Phe Thr Trp

245 250 255

Leu Asn Thr Phe Asp Val Lys Tyr Glu Ile Leu Ala His Arg Glu Asn

260 265 270

Ile Asn Leu Gly Leu Val Asn Met Gln Val Tyr Asn Pro Gly Asn Glu

275 280 285

Ser Val Gln Val Ile Val Ser Asp Val Leu Asp Phe Asn Ser Ser Gln

290 295 300

Arg Cys Gln Leu Asn Gln Ile Ser His Asp Lys Asn Gly Ile Tyr Val

305 310 315 320

Thr Phe His Pro Gln Gly Leu Ser Tyr Ile Asp Gly Ala Ile Tyr Ser

325 330 335

Thr Leu Ser Ala Asn Gly Gln Ile Thr Arg Glu Gln Thr Asn Glu Thr

340 345 350

Val Phe Gln Asn Val Glu Leu Thr Ile Glu Pro His Ser Cys Val Gln

355 360 365

Val Ala Lys Tyr Val Gly Ile Ala Thr Thr Asp Leu Asp Pro Asp Ser

370 375 380

Phe Lys Thr Ala Asp Asp Val Leu Lys Phe Ala Arg Lys Val Ser Gln

385 390 395 400

Asn Lys Lys His Gly Asp Ala Thr Gln Leu Val Asn Ser His Arg Ser

405 410 415

Ala Trp Ser Lys Ile Ile Gln Asp Ala Pro Leu Val Thr Phe Pro Ser

420 425 430

Asp Ser Leu Leu Asn Leu Gly Ala Arg Ala Ser Ile Phe His Leu Leu

435 440 445

Ala Asn Thr Arg Pro Asn Ala Glu Gly Val Thr Gly Ala Leu Gly Val

450 455 460

Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Thr

465 470 475 480

Asp Leu Trp Met Leu Asn Gly Ile Leu Pro Phe Ala Pro Asp His Ile

485 490 495

Lys Ser Phe Ile Asn Tyr Arg Val His Leu His Gln Gln Ala Ile Asp

500 505 510

Asn Val Pro Arg Gly Tyr Gln Gly Ala Val Tyr Pro Trp Thr Ser Gly

515 520 525

Arg Phe Gly Asn Cys Thr Gly Thr Gly Pro Cys Leu Asp Tyr Glu Tyr

530 535 540

His Ile Asn Met Ala Val Ala Met Ala Ser Trp Gln Leu Tyr Ile Ser

545 550 555 560

Gly Ala Ala Asp Asp Thr Phe Leu Glu Ser Val Ala Tyr Pro Ile Ile

565 570 575

Asn Asp Ala Ala Ser Phe Leu Ala Glu Tyr Val Val His Tyr Asn Asp

580 585 590

Thr Leu Gly Lys Tyr Thr Thr Lys Asn Leu Thr Asp Pro Asp Glu Phe

595 600 605

Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn Thr Gly Ile Val Leu

610 615 620

Val Met Arg Trp Ala Gln Thr Ala Gly Ser Ile Leu Gly Lys Gln Val

625 630 635 640

Pro Lys Ile Tyr His Asp Ile Glu Thr Ala Met Phe Leu Pro Thr Ala

645 650 655

Glu Asn Thr Gln Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser

660 665 670

Val Gly Ile Lys Gln Ala Asp Val Ile Met Met Thr Tyr Pro Leu Glu

675 680 685

Asn Glu Leu Ile Asp Gln Asp Gln Ala Tyr Ile Asn Met Glu Phe Tyr

690 695 700

Ser Met Lys Gln Val Gly Tyr Gly Pro Ala Met Thr Phe Pro Ile Phe

705 710 715 720

Ser Ile Val Ala Ser Asn Leu Ala Phe Thr Gly Cys Ala Ser Gln Ser

725 730 735

Tyr Leu His Lys Ala Ile Gln Pro Phe Leu Arg Gly Pro Phe Ala Gln

740 745 750

Phe Ala Glu Gln Asn Asn Asp Asp Tyr Leu Thr Asn Gly Gly Thr His

755 760 765

Pro Ala Phe Pro Phe Leu Thr Ala His Gly Gly Phe Leu Gln Ala Ile

770 775 780

Leu Gln Gly Leu Thr Gly Met Arg Phe Asp Tyr Thr Phe Asp Asn Asn

785 790 795 800

Lys Leu Gln Arg Leu Leu Lys Leu Asp Pro Ile Ala Leu Pro Cys Leu

805 810 815

Gly Glu Gly Val Arg Phe Asp Ser Ile Lys Tyr Asp Asn His Thr Leu

820 825 830

Ser Met Ala Ile Asn Glu Thr His Phe Thr Ile Lys Asn Lys Gly Lys

835 840 845

Thr Thr Pro Asn Ala Arg Asn Tyr Val Thr Ile Leu Leu Ala Glu Arg

850 855 860

Asn Ala Met His Gly Lys Tyr Thr Ile Asn Asp Glu Asp Glu Gln Ser

865 870 875 880

Phe Pro Leu Phe Glu Thr Ser Glu Ser Phe Pro Asp Ser Ile Ser Glu

885 890 895

Cys Asn Lys Ala Gly Phe Phe Asn Ile Thr Glu Gly Ala Tyr Gly Asp

900 905 910

Val Ser Ile Ser Ile Asn Asp Gly Asp Asn Thr Thr Ser Trp Gln Ala

915 920 925

Lys Tyr Asn Asp Thr Thr Gly Lys Val Leu Val Asp Leu Lys Ser Phe

930 935 940

Arg Asn Ile Ser Ser Gly Thr Phe Ile Trp Gly Asp Lys Pro Pro Lys

945 950 955 960

Arg Val Lys Val Ser Lys Tyr Ser Gly Ser Ser Phe Thr Ala Val Thr

965 970 975

Asp Phe Phe Ala Gln Val Asp Phe Gly Asn Glu Leu Phe Asn Glu Tyr

980 985 990

Lys Tyr Ala Asn Pro Glu Gly Lys Leu His Asn Gln Ser Asp Val Phe

995 1000 1005

Glu Glu Val Tyr Ser Gly Asp Val Lys Ile Ser Ala Pro Phe Asp

1010 1015 1020

Pro Glu Glu Tyr Phe Gln Val Trp Val Pro Thr Arg His Asn Ile

1025 1030 1035

Thr Glu Val Ala Val Asn Leu Gln Thr Arg Phe Leu Leu Ile Glu

1040 1045 1050

Val Asp Glu Ile His Asn Thr Glu Ala Ile Asp Gly Asp Tyr Gly

1055 1060 1065

Gly Ala Lys Leu Ala Glu Val Val Phe Tyr

1070 1075

<210> 56

<211> 1140

<212> PRT

<213> 长孢洛德酵母(Lodderomyces elongisporus)

<400> 56

Met Val Ser Asn Glu Glu Asp Ile Tyr Ile Asn His Asp Pro Asn Pro

1 5 10 15

Asn Phe Asn Ser Asp Ser Asn Ser Asn Ser Asn Ser Asp Pro Asp Pro

20 25 30

Asn Tyr Asn Phe Ile Ser Arg Phe Thr Ser Thr Pro Ser Ile Asn Ser

35 40 45

Asp Ser His Leu Gly Ser His Pro Asn Ser Thr Phe Gln Leu Tyr Leu

50 55 60

Asn Arg Ala Lys Gly Lys Ser Ser Met Ile Ile Met Ala Leu Ile Ala

65 70 75 80

Asn Phe Ile Leu Phe Tyr Ser Leu His Leu Leu Asn Val Arg Phe Cys

85 90 95

Glu Ala Phe Pro Tyr Leu Val Pro Glu Val Glu Lys Glu Ser Val Phe

100 105 110

Asn Asn Ile Phe Ser Ser Leu Glu Gly Leu Glu Asp Leu Val Asn Ser

115 120 125

Tyr Glu Asn Lys Asp Ile Phe Ser Gln Leu Lys Tyr Ser Gly Gln Ala

130 135 140

Phe Tyr Asp Asn Glu Leu Asn Val Val Gly Thr Leu Glu Phe Pro Leu

145 150 155 160

Tyr Asn Lys Tyr Gln Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly

165 170 175

Ser Arg Ile Pro Asn Leu Gly His Gly Phe Thr Tyr Asp Gln Leu Pro

180 185 190

Asp Thr Cys Gln Tyr Cys Glu Asp Asp Leu Ser Asn Gly Trp Pro Leu

195 200 205

Phe Asn Lys Arg Phe Ala Gly Ala Phe Ile Ala Gly Phe Tyr Asp Leu

210 215 220

Gln Glu Asn Thr Thr Gly Thr Asn Phe Pro Glu Leu Leu Glu Asn Gly

225 230 235 240

Tyr Glu Ser Val Ile Ala Ala Val Pro Gln Trp Thr Thr Leu Asn Val

245 250 255

Ala Ile Glu Ala Asn Gly Gln Asn Tyr Thr Leu Asp Pro Ser Leu Glu

260 265 270

Thr His Glu Ile Gly Asn Ile Thr Asn Tyr Val Gln Asn Met Ser Leu

275 280 285

Ser Asn Gly Ile Val Thr Thr Gln Tyr Thr Trp Leu His Asn Ile Asp

290 295 300

Ile Lys Tyr Thr Val Val Ala His Arg Ala Gln Pro Asn Leu Gly Leu

305 310 315 320

Val Thr Leu Asp Ile Gln Ser Arg Ser Asp Glu Val Val Asn Leu Arg

325 330 335

Val Glu Asp Ile Leu Asp Phe Asn Thr Ser Gln Arg Cys Glu Leu Thr

340 345 350

Ala Leu Gly His Asp Glu Leu Gly Ile Tyr Ile Thr Phe Gln Pro Glu

355 360 365

Asn Val Asp Ser Ala His Gly Ala Ile Tyr Ser Arg Leu Ile Glu Ser

370 375 380

Leu Glu Ser Ala Gly Ala Thr Gly Ala Ser Ala Val Ser Asn Leu Glu

385 390 395 400

Lys Val Ser Asp Gly Glu Arg Ile Ser Gln Ile Ala Lys Ile Thr Leu

405 410 415

Glu Pro Phe Glu Ser Thr Gln Ile Asn Lys Val Val Gly Ile Val Ser

420 425 430

Ser Asp Ser Asp Pro Glu Asn Leu Gly Thr Ala Lys Ser Val Arg Glu

435 440 445

Ser Ala Lys Glu Ala Ala Gln Lys Tyr Asn Asn Ile Gln Asp Ile Leu

450 455 460

Thr Thr His Asn Asp Ala Trp Thr Lys Leu Leu Gly Glu Asn Pro Arg

465 470 475 480

Val Thr Phe Pro Ser Glu Pro Leu Leu Asn Leu Ala Ala Arg Ala Ser

485 490 495

Val Tyr His Leu Leu Ala Asn Thr Arg Pro Gln Ala Phe Gly Leu Thr

500 505 510

Gly Ala Val Gly Val Gly Gly Leu Ser Ser Asp Ser Tyr Ala Gly Met

515 520 525

Val Phe Trp Asp Thr Asp Leu Trp Met Phe Asn Gly Ile Leu Pro Leu

530 535 540

Met Pro Ser His Ala Lys Ser Ile Val Asn Tyr Arg Leu His Leu His

545 550 555 560

Glu Gln Ala Val Gln Asn Val Pro Glu Gly Tyr Asn Gly Ala Val Tyr

565 570 575

Pro Trp Thr Ser Gly Arg Tyr Gly Asn Cys Thr Ala Thr Gly Pro Cys

580 585 590

Leu Asn Tyr Glu Tyr His Ile Asn Thr Ala Val Ala Met Ala Ala Trp

595 600 605

Glu Leu Tyr Ile Ser Gly Ala Ser Asp Glu Leu Phe Leu Arg Glu Tyr

610 615 620

Ala Ser Thr Leu Ile Asn Asn Ala Ala Thr Phe Phe Ser Asp Tyr Leu

625 630 635 640

Val Gln Trp Asn Asp Thr Leu Gly Gln Tyr Thr Thr Ser Asn Leu Thr

645 650 655

Asp Pro Asp Glu Tyr Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn

660 665 670

Ser Gly Ile Ser Leu Leu Met Gln Trp Ala Thr Met Val Gly His Ile

675 680 685

Leu Gly Glu Asp Val Pro Asp Val Tyr Ser Gln Ile Ala Glu Asn Leu

690 695 700

His Ile Pro Ser Ala Asn Asn Ser Gln Asn Ile Thr Leu Glu Tyr Ser

705 710 715 720

Gly Met Asn Ser Thr Val Gly Ile Lys Gln Ala Asp Val Ile Met Met

725 730 735

Thr Tyr Pro Leu Ala Asn Asn Leu Ile Asp Glu Glu Gln Gly Tyr Ile

740 745 750

Asn Met Glu Tyr Tyr Ser Met Arg Gln Val Ser Tyr Gly Pro Ala Met

755 760 765

Thr Phe Pro Ile Phe Ser Ile Val Ala Ser Lys Leu Ala Val Ser Gly

770 775 780

Cys Ala Ser Gln Ser Tyr Leu His Lys Ala Val Gln Pro Tyr Leu Arg

785 790 795 800

Gly Pro Phe Ala Gln Phe Ser Glu Gln Asn Asn Asp Asp Tyr Lys Thr

805 810 815

Asn Gly Gly Thr His Pro Ala Phe Pro Phe Leu Thr Ala His Gly Gly

820 825 830

Phe Leu Gln Ala Ala Leu His Gly Leu Thr Gly Met Arg His Arg Leu

835 840 845

Asn Val Ser Asp Thr Gly Val Ile Glu Arg Phe Leu Phe Leu Asp Pro

850 855 860

Ile Ala Leu Pro Cys Leu Gly Glu Gly Val Leu Phe Asn Ser Val Phe

865 870 875 880

Tyr Asn Asn His Thr Ile Ser Met Ser Ile Asn Gln Thr His Phe Thr

885 890 895

Ile Glu Asn Asn Gly Lys Thr Ser Asp Ser Gln Asn Ala Asp Gly Phe

900 905 910

Ile Thr Leu Gln Leu Ala Glu Arg Asn Pro Asp His Gly Thr Phe Val

915 920 925

Leu Leu Asp Gly Glu Lys Arg Ser Phe Ala Leu Tyr Lys Pro Glu Lys

930 935 940

Ser Phe Val Asp Ser Ile Ser Glu Cys Gly Asp Ala Ser Phe Tyr Asn

945 950 955 960

Ile Thr Glu Gly Ala Tyr Gly Val Thr Pro Ile Ser Ile Asn Asp Gly

965 970 975

Asp Asn Thr Thr Arg Trp Gln Ala Lys Thr Asn Asp Thr Val Gly Lys

980 985 990

Ile Leu Val Asp Leu His Ser Ile Lys Asn Val Ser Ser Val Leu Phe

995 1000 1005

Asn Cys Ala Asp Arg Pro Pro Leu Ser Leu Lys Val Ser Lys Tyr

1010 1015 1020

Ile Gly Asp Lys Phe Thr Gln Val Thr Asp Phe Leu Ser Gln Val

1025 1030 1035

Asp Phe Gly Asn Glu Val Tyr Lys Asn Tyr Arg Tyr Ala Asn Pro

1040 1045 1050

Asn Asp Lys Leu Tyr Asn Gln Ser Glu Val Phe Glu Glu Val Ser

1055 1060 1065

Ser Gln Asp Val Ser Ile Ser Glu Pro Phe Ser Gln Glu Glu Phe

1070 1075 1080

Asp Gln Val Leu Val Pro Val Arg His Asn Ala Thr Leu Leu Asp

1085 1090 1095

Leu Glu Ile Gln Gln Cys Arg Phe Ile Leu Ile Glu Val Glu Gly

1100 1105 1110

Val His Asp Asn Ser Ser Leu Glu Glu Asp Gly Asp Glu Ser Tyr

1115 1120 1125

Ser Asp Gly Ala Lys Leu Ala Glu Val Ala Phe Phe

1130 1135 1140

<210> 57

<211> 1078

<212> PRT

<213> 杜氏假丝酵母(Candida dubliniensis)

<400> 57

Met Ala Ala Asn Pro Ser Phe Ile Leu Val Asp Thr Arg Arg Pro His

1 5 10 15

Asn Gln Ser Phe Ile Gln Phe Cys Ile His Ala Thr Ser Glu Lys Lys

20 25 30

Ser Trp Ile Ala Leu Met Cys Leu Ala Asn Leu Leu Leu Leu Phe Ser

35 40 45

Phe His Leu Leu Tyr Ala Arg Leu Cys Asn Gly Phe Pro Tyr Val Val

50 55 60

Pro Ser Thr Ala Arg Ser Thr Asn Pro Ile His Tyr Thr Asn Leu Glu

65 70 75 80

Gln Leu Val Asn Ser Pro Glu Asn Lys Gln Ile Phe Asn Gln Leu Lys

85 90 95

Phe Ser Asp Gln Ala Phe Tyr Asp Pro His Asn Asn Val Val Gly Thr

100 105 110

Ile Glu Phe Pro Leu Phe Asn Gln Tyr Gln Arg Gln Pro Tyr Val Thr

115 120 125

Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Leu Gly Gln Gly Phe Thr

130 135 140

Tyr Asp Gln Leu Thr Asn Ser Ser Thr Ala Asn Asp Asp Asp Leu Leu

145 150 155 160

Asn Gly Trp Pro Leu Phe Asn Lys Arg Tyr Ser Gly Ala Phe Val Ala

165 170 175

Gly Phe Tyr Asp Leu Gln Lys Asn Thr Thr Gly Thr Asn Phe Ala Glu

180 185 190

Leu Leu Glu Asn Gly Tyr Glu Ser Val Ile Ala Ala Val Pro Gln Trp

195 200 205

Thr Ala Leu Ser Leu Asn Val Glu Leu Leu Gly Lys Lys Tyr Thr Leu

210 215 220

Asp Pro Ser Leu Glu His Glu Val Ile Gly Asn Ile Ser Asn Tyr Val

225 230 235 240

Gln Asn Met Ser Leu Ser Asp Gly Ile Val Thr Thr Gln Phe Thr Trp

245 250 255

Leu Asn Ser Ile Asp Val Lys Tyr Glu Val Leu Ala His Arg Glu Asn

260 265 270

Ile Asn Leu Gly Leu Val Asn Met Gln Ile Tyr Asn Ser Gly Asn Glu

275 280 285

Ser Val Gln Val Val Val Ser Asp Val Leu Asp Phe Ser Ser Ser Gln

290 295 300

Arg Cys Gln Leu Asn Lys Val Ala His Asp Lys Asn Gly Ile Tyr Val

305 310 315 320

Thr Phe Asn Pro Gln Gly Leu Ser Tyr Ile Asp Gly Ala Ile Tyr Ser

325 330 335

Thr Leu Ser Val Asp Gly Asp Ile Thr Arg Glu Gln Ile Asn Glu Thr

340 345 350

Val Ser Gln Lys Ala Lys Leu Thr Met Glu Pro His Ser Cys Val Gln

355 360 365

Ala Ala Lys Phe Ile Gly Ile Ala Thr Thr Asp Phe Asp Pro Asp Ser

370 375 380

Phe Lys Asn Ala Asp Asp Val Leu Lys Phe Ala Arg Glu Val Ser Gln

385 390 395 400

Asn Lys Asn His Gly Asp Ala Ser Gln Leu Ile Ser Ser His Arg Ser

405 410 415

Ala Trp Ser Lys Ile Ile Gln Asp Thr Pro Leu Ile Thr Phe Pro Ser

420 425 430

Asp Pro Leu Leu Asn Leu Gly Ala Arg Ala Ser Ile Phe His Leu Leu

435 440 445

Ala Asn Thr Arg Pro Asn Ala Glu Gly Val Thr Gly Ala Leu Gly Val

450 455 460

Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Thr

465 470 475 480

Asp Leu Trp Met Leu Asn Gly Ile Leu Pro Phe Ala Pro Asp His Ile

485 490 495

Lys Ser Phe Ile Asn Tyr Arg Val His Leu His Gln Gln Ala Ile Asp

500 505 510

Asn Val Pro Ser Gly Tyr Gln Gly Ala Val Tyr Pro Trp Thr Ser Gly

515 520 525

Arg Phe Gly Asn Cys Thr Gly Thr Gly Pro Cys Leu Asp Tyr Glu Tyr

530 535 540

His Ile Asn Met Ala Val Ala Met Ala Ser Trp Gln Leu Tyr Val Ser

545 550 555 560

Gly Ala Ala Asp Asp Thr Phe Leu Glu Ser Val Ala Tyr Pro Ile Ile

565 570 575

Asn Asp Ala Ala Ser Phe Leu Ala Glu Tyr Val Val His Tyr Asn Asp

580 585 590

Thr Leu Gly Lys Tyr Thr Thr Lys Asn Leu Thr Asp Pro Asp Glu Phe

595 600 605

Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn Thr Gly Ile Val Leu

610 615 620

Val Met Arg Trp Ala Gln Met Val Gly Arg Ile Leu Gly Lys Glu Val

625 630 635 640

Pro Arg Val Phe His Asp Ile Glu Thr Ala Met Phe Leu Pro Thr Ala

645 650 655

Glu Asn Ser Gln Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser

660 665 670

Val Gly Ile Lys Gln Ala Asp Val Ile Met Met Thr Tyr Pro Leu Glu

675 680 685

Asn Glu Leu Ile Asp Lys Asp Gln Ala Tyr Ile Asn Met Glu Phe Tyr

690 695 700

Ser Met Lys Gln Val Gly Tyr Gly Pro Ala Met Thr Phe Pro Ile Phe

705 710 715 720

Ser Ile Val Ala Ala Asn Leu Ala Phe Thr Gly Cys Ala Ser Gln Ser

725 730 735

Tyr Leu His Lys Ala Ile Gln Pro Phe Leu Arg Gly Pro Phe Ala Gln

740 745 750

Phe Ala Glu Gln Asn Asn Asp Asn Tyr Leu Thr Asn Gly Gly Thr His

755 760 765

Pro Ala Phe Pro Phe Leu Thr Ala His Gly Gly Phe Leu Gln Ala Ile

770 775 780

Leu Gln Gly Ile Thr Gly Met Arg Phe Asp Tyr Thr Phe Glu Asp Asn

785 790 795 800

Arg Leu Gln Arg Ile Leu Thr Leu Asp Pro Ile Ala Leu Pro Cys Leu

805 810 815

Gly Gly Gly Val Arg Phe Asp Gly Ile Lys Phe Asp Asn His Thr Leu

820 825 830

Ser Met Ala Ile Asn Glu Thr His Phe Ala Ile Arg Asn Asn Gly Lys

835 840 845

Ala Thr Pro Asn Ala Gln Asn His Val Thr Ile Leu Leu Ala Glu Arg

850 855 860

Asn Pro Met His Gly Lys Tyr Thr Ile Asn Asp Lys Asp Glu Leu Ser

865 870 875 880

Phe Pro Leu Phe Glu Thr Ser Glu Ser Phe Pro Asp Ser Val Ser Glu

885 890 895

Cys Ser Lys Ala Gly Phe Phe Asn Ile Thr Glu Gly Ala Tyr Gly Asp

900 905 910

Val Ser Ile Ser Ile Asn Asp Gly Asp Asn Thr Thr Ser Trp Gln Thr

915 920 925

Lys Tyr Asn Asp Thr Thr Gly Lys Val Leu Val Asp Leu Lys Ser Leu

930 935 940

Lys Asn Ile Ser Ser Gly Thr Phe Ile Trp Gly Asp Lys Pro Pro Lys

945 950 955 960

Arg Leu Lys Ile Ser Lys Tyr Ser Gly Asp Gly Phe Ala Thr Val Thr

965 970 975

Asp Phe Phe Ala Gln Val Asp Phe Gly Asn Lys Leu Phe Asn Glu Tyr

980 985 990

Lys Tyr Thr Asn Pro Glu Gly Lys Leu His Asn Gln Ser Asp Val Phe

995 1000 1005

Glu Glu Val Tyr Ser Gly Asp Val Lys Ile Ser Ala Pro Phe Asp

1010 1015 1020

Pro Glu Glu Tyr Phe Gln Val Trp Val Pro Thr Arg His Asn Ile

1025 1030 1035

Thr Glu Leu Thr Leu Asn Leu Gln Thr Arg Phe Leu Leu Ile Glu

1040 1045 1050

Val Asp Glu Ile His Asn Thr Glu Pro Ile Asp Gly Asp Tyr Gly

1055 1060 1065

Gly Ala Lys Leu Ala Glu Val Val Phe Tyr

1070 1075

<210> 58

<211> 1094

<212> PRT

<213> 木糖发酵酵母(Spathaspora passalidarum)

<400> 58

Met Val Arg Lys Lys Thr Gln Tyr Gln Ser Leu Asn Ile Ala Pro Gly

1 5 10 15

Pro Gly Glu Thr Asn Asn Pro Thr Phe Phe Glu Tyr Leu Phe Ser Ser

20 25 30

Ile Arg His His Lys Gly Ile Val Thr Met Val Ile Phe Ala Asn Leu

35 40 45

Ile Thr Leu Val Ser Ile His Leu Leu Phe Leu Lys Tyr Ala Asn Ala

50 55 60

Phe Pro His Ile Ala Arg Asn Gln Asp Glu Phe Gly Thr Ile Lys Asn

65 70 75 80

Asn Pro Ser Lys Asp Gln Gln Ser Pro Phe Ser Asp Gln Ser Gly Leu

85 90 95

Ile Asn Leu Ile Asn Ser Pro Gln Asn Lys Ala Ile Tyr Ser Gln Met

100 105 110

Lys Tyr Ser Ser Tyr Ala Phe Tyr Asp Asn Thr Thr Asn Thr Val Gly

115 120 125

Thr Ile Glu Tyr Val Pro Phe Asn Gln Tyr Gln Arg Gln Pro Tyr Val

130 135 140

Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Leu Gly His Gly Phe

145 150 155 160

Thr Tyr Asp Gln Leu Ser Asp Ala Pro Asp Ala Asn Asp Asp Asp Leu

165 170 175

Leu Asn Gly Trp Pro Leu Phe Asn Gln Arg Tyr Ala Gly Ala Phe Ile

180 185 190

Ala Gly Phe Phe Asp Ile Gln Lys Asn Thr Ser Gly Thr Asn Phe Pro

195 200 205

Glu Leu Leu Asp Asn Gly Tyr Glu Ser Val Ile Ala Ala Val Pro Gln

210 215 220

Trp Thr Thr Leu Lys Leu Ser Thr Thr Lys Asp Asn Gln Asn Tyr Thr

225 230 235 240

Leu Asp Pro Ser Leu Pro Pro Ala Gln Ile Gly Ser Ile Ser Asn Tyr

245 250 255

Val Gln Asn Leu Ser Leu Ser Asn Gly Ile Val Thr Thr Gln Tyr Thr

260 265 270

Trp Leu Asp Ser Ile Glu Val Lys Tyr Glu Ile Leu Ala His Arg His

275 280 285

Asn Ile His Leu Gly Val Val Asn Leu Glu Val Tyr Asn Ser Gly Asn

290 295 300

Glu Thr Val Arg Leu Asp Ile Thr Asp Glu Ile Asp Phe Asn Ser Ala

305 310 315 320

Gln Arg Ser Gln Leu Thr Gly Val Asn Thr Asp Asp Lys Gly Ile Tyr

325 330 335

Val Thr Phe Gln Pro Ser Glu Ile Asp Tyr Val Asn Gly Ala Ile Tyr

340 345 350

Ser Thr Leu Ile Val Asp Ser Pro Ile Thr Arg Gln Thr Thr Asn Gly

355 360 365

Thr Thr Ala Gln Gln Ile Ala Thr Ile Tyr Leu Gln Pro His Glu Ser

370 375 380

Leu Ser Ala Asn Lys Phe Val Gly Ile Ala Ser Ser Asp Leu Asn Pro

385 390 395 400

Asp Gln Leu Thr Thr Thr Asp Ser Val Leu Asn Ile Ala Lys Glu Val

405 410 415

Ser Gln Lys His Gln Lys Leu Lys Pro Val Val Lys Ser His Asn Glu

420 425 430

Gln Trp Lys Lys Thr Leu Gly Glu Asn Pro Leu Val Thr Phe Pro Asp

435 440 445

Ala Pro Leu Leu Asn Leu Gly Ser Arg Ala Ser Ile Tyr His Leu Ile

450 455 460

Ala Asn Thr Arg Pro Asp Ala Gln Gly Val Thr Ala Ala Leu Gly Val

465 470 475 480

Gly Gly Leu Ser Ser Asp Ser Tyr Ala Gly Leu Val Phe Trp Asp Ala

485 490 495

Asp Leu Trp Met Ser Asn Ala Leu Leu Pro Phe Ala Pro Asp His Ala

500 505 510

Lys Ser Ile Val Asn Tyr Arg Leu Tyr Thr His Ala Gln Ala Val Lys

515 520 525

Asn Met Pro Ala Gly Tyr Glu Gly Ala Val Tyr Pro Trp Thr Ser Ala

530 535 540

Arg Phe Gly Asn Cys Thr Gly Thr Gly Pro Cys Leu Asp Tyr Glu Tyr

545 550 555 560

His Ile Asn Val Ala Val Ala Lys Ala Ala Trp Asn Tyr Tyr Leu Ser

565 570 575

Gly Ala Gly Asp Glu Asp Tyr Leu Ala Asp Val Ala Tyr Pro Ile Ile

580 585 590

Asn Asp Ala Ala Thr Phe Phe Ser Gly Tyr Leu Ala Gln Tyr Asn Glu

595 600 605

Ser Val Asn Arg Tyr Thr Thr His Asn Leu Thr Asp Pro Asp Glu Tyr

610 615 620

Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn Ala Gly Ile Ser Leu

625 630 635 640

Val Met Lys Trp Ala Ile Ala Leu Gly Asn His Leu Gly Lys Asp Val

645 650 655

Pro Gln Ile Phe Gln Asn Ile Ala Gly Asn Met Tyr Ile Pro Ser Ala

660 665 670

Asn Asn Ser Gln Glu Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser

675 680 685

Val Gly Ile Lys Gln Ala Asp Val Ile Met Met Thr Tyr Pro Leu Asp

690 695 700

Asn Glu Leu Leu Asp Leu Glu Gln Ala Tyr Glu Asn Met Glu Phe Tyr

705 710 715 720

Ser Met Lys Gln Val Ser Tyr Gly Pro Ala Met Thr Phe Pro Ile Phe

725 730 735

Ser Ile Val Ala Ala Asn Leu Ala Pro Thr Gly Cys Ala Ser Gln Ser

740 745 750

Tyr Leu Gln Lys Ala Ile Gln Pro Phe Leu Arg Gly Pro Phe Ala Gln

755 760 765

Phe Ser Glu Gln Asn Asn Asp Asn Phe Leu Ile Asn Gly Gly Thr His

770 775 780

Pro Ala Phe Pro Phe Leu Thr Ala His Gly Gly Phe Thr Gln Ala Val

785 790 795 800

Leu Gln Gly Leu Thr Gly Met Arg Tyr Asp Tyr Phe Ile Gln Asn Gly

805 810 815

Thr Leu Val Arg Ala Leu Arg Val Asp Pro Ile Ala Leu Pro Cys Leu

820 825 830

Gly Ser Gly Val Gln Tyr Glu Gly Ile Tyr Tyr Asn Asn His Ser Val

835 840 845

Ala Met Ser Ile Asn Glu Thr His Phe Thr Ile Gln Asn Arg Gly Pro

850 855 860

Thr Glu Lys Ser Ala Gly Lys Tyr Ile Asn Ile Ile Val Asp Asp Arg

865 870 875 880

Asn Pro Lys Gln Gly Gln Tyr Ala Leu Asp Asp Tyr Asp Glu Met Val

885 890 895

Ile Pro Leu Tyr Thr Pro Ile Ala Ser Tyr Pro Asp Ser Ile Ser Glu

900 905 910

Cys Gly Leu Ala Ser Phe Tyr Asn Ile Thr Glu Gly Ala Tyr Gly Asp

915 920 925

Leu Pro Ile Ser Ile Asn Asp Gly Asp Asn Thr Thr Arg Trp Gln Val

930 935 940

Lys Asn Asn Asp Thr Ile Gly Lys Ile Leu Val Asp Leu Lys Ser Phe

945 950 955 960

Lys Asn Ile Ser Asn Ile Leu Phe Asn Trp Arg Asp Lys Pro Pro Gln

965 970 975

Thr Leu Arg Leu Ser Val Tyr Asn Asp Asn Gln Phe Thr Asp Ala Thr

980 985 990

Asp Phe Phe Ser Lys Val Asp Phe Gly Asn Gly Leu Tyr Asn Asp Tyr

995 1000 1005

Lys Tyr His Asn Pro Asp Ser Gln Ile Val Asn Gln Ser Asp Val

1010 1015 1020

Phe Thr Glu Val Tyr Ser Ala Ser Val Asp Ile Thr Ala Pro Tyr

1025 1030 1035

Asp Ser Asn Glu Phe Asn Glu Val Met Val Pro Asp Arg Ser Asn

1040 1045 1050

Ile Thr Ser Leu Asn Leu Asn Leu Tyr Asn Arg Phe Leu Leu Ile

1055 1060 1065

Glu Val Asn Gly Ile His Asp Ala Glu Pro Val Glu Gly Asp Thr

1070 1075 1080

Gly Gly Ala Lys Leu Ala Glu Val Thr Phe Phe

1085 1090

<210> 59

<211> 1083

<212> PRT

<213> 树干毕赤酵母(Scheffersomyces stipitis)

<400> 59

Met Val Val Val Ser Lys Trp Arg Phe Ser Asp Val Ile His Thr Val

1 5 10 15

Cys Asp Thr Ile Val Asp Thr Phe Tyr Arg Lys Gln Ala Phe Val Lys

20 25 30

Thr Met Val Phe Val Asn Val Gly Leu Ile Leu Leu Leu His Trp Tyr

35 40 45

Phe Val Ser Leu Ser Gln Ala Phe Pro Leu Gln Leu Arg Asn His Val

50 55 60

Pro Gly Thr Val Thr His Glu Ser Val Ala Glu Glu Arg Ala Arg His

65 70 75 80

Leu Lys Leu Val Asn Ser Pro Gln Asn Lys Ala Val Tyr Thr Gln Leu

85 90 95

Lys Tyr Ala Ala Asn Ala Phe Phe Asp Val Asp Thr Asn Thr Ile Gly

100 105 110

Thr Thr His Phe Thr Pro Tyr Asn Gln Tyr Gln Arg Gln Pro Tyr Val

115 120 125

Ala Asn Gly Tyr Ile Gly Ala Arg Ile Pro Asn Leu Gly Gln Gly Phe

130 135 140

Thr Tyr Asp Gln Ile Ser Asp Ser Ala Asp Ala Thr Asn Asp Asp Leu

145 150 155 160

Leu Asn Gly Trp Pro Leu Phe Asn Glu Arg Tyr Ser Gly Ala Phe Val

165 170 175

Ala Gly Phe Phe Asp Ile Gln Lys Asn Thr Thr Gly Thr Asn Phe Pro

180 185 190

Glu Leu Leu Ala Asn Gly Tyr Glu Ser Val Ile Ala Ser Val Pro Gln

195 200 205

Trp Thr Thr Leu Gln Val Ser Thr Ile Lys Asp Gly Val Asp Phe Ser

210 215 220

Leu Asp Pro Val Asn Ser Val Asp Met Asn Asn Val Ile Ser Ser Tyr

225 230 235 240

Gly Gln Asn Leu Ser Leu Val Asn Gly Ile Val Thr Thr Glu Tyr Thr

245 250 255

Trp Leu Asp Asp Ile Arg Ile Lys Tyr Lys Ile Leu Ala His Arg Lys

260 265 270

Glu Ile Asn Leu Gly Leu Val Glu Leu Ser Ile Ser Asn Met Gly Asn

275 280 285

Thr Ser Leu Thr Phe Asn Val Thr Asp Val Leu Asp Ala Ser Thr Ala

290 295 300

Gln Arg Ser Gln Leu Thr Gly Val Asn Ser Asp Gly Lys Gly Ile Tyr

305 310 315 320

Ile Thr Phe Ser Pro Asn Glu Leu Asn Tyr Ile Asn Gly Ala Ile Tyr

325 330 335

Ser Thr Leu His Val Glu Asp Gly Ser Ser Ile Gln Ser Ser Ser Ser

340 345 350

Thr Ser Lys Val Ser Gln Tyr Val Glu Val Thr Val Asn Pro Gly Arg

355 360 365

Thr Ser Arg Val Val Lys Leu Val Gly Val Ala Thr Thr Asp Leu Asp

370 375 380

Pro Arg Asn Leu Asp Ser Leu Asp Lys Val Leu Ala Phe Ala Lys Lys

385 390 395 400

Val Ser Gln Thr Tyr Thr Asn Ala Asp Asp Val Val Glu Ser His Leu

405 410 415

Val Ala Trp Ala Gln Thr Leu Glu Ser Thr Pro Ala Ile Thr Phe Ala

420 425 430

Asp Asp Arg Leu Leu Thr Leu Ala Ser Arg Ala Ser Leu Phe His Leu

435 440 445

Thr Ala Asn Thr Arg Pro Asp Ala Asn Gly Val Thr Ala Ala Met Gly

450 455 460

Val Ala Gly Leu Ser Ser Asp Ser Tyr Ala Gly Met Val Phe Trp Asp

465 470 475 480

Ala Asp Ile Trp Met Met Ser Gly Leu Leu Pro Phe Ile Pro Ser His

485 490 495

Ala Lys Ser Ile Val Asn Tyr Arg Met His Thr His Asp Gln Ala Ile

500 505 510

Lys Asn Leu Pro Glu Gly Ala Lys Gly Ala Val Tyr Pro Trp Thr Ser

515 520 525

Gly Arg Phe Gly Asn Cys Thr Ala Thr Gly Pro Cys Leu Asp Tyr Glu

530 535 540

Tyr His Ile Asn Val Ala Val Ala Met Ser Ala Trp Gln Leu Tyr Leu

545 550 555 560

Ser Gly Ala Ala Asp Glu Gln Phe Leu Ala Asp Val Val Tyr Pro Leu

565 570 575

Val Asn Asp Ala Ala Glu Phe Phe Ala Asp Tyr Val Val Thr Tyr Asp

580 585 590

Asp Thr Leu Lys Gln Phe Thr Thr His Asn Leu Thr Asp Pro Asp Glu

595 600 605

Tyr Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn Ser Gly Ile Ser

610 615 620

Leu Val Glu Lys Trp Ala Ile Gln Ile Ser Asn His Leu Gly Lys Glu

625 630 635 640

Phe Pro Ser Gln Tyr Ser Asn Ile Val Gly Asn Met His Leu Pro Thr

645 650 655

Ser Gly Asn Ser Asp Asn Ile Thr Leu Glu Tyr Thr Gly Met Asn Ser

660 665 670

Ser Val Gly Ile Lys Gln Ala Asp Val Ile Met Ile Thr Tyr Pro Leu

675 680 685

Gln Asn Glu Leu Ile Ser Glu Ala Gln Ala Leu Thr Asn Met Glu Phe

690 695 700

Tyr Ser Val Lys Gln Val Asn Tyr Gly Pro Ala Met Thr Phe Pro Ile

705 710 715 720

Phe Ser Ile Val Ala Ser His Val Ser Thr Ser Gly Cys Ala Ser Gln

725 730 735

Ser Tyr Leu Gln Lys Ala Val Gln Pro Phe Leu Arg Gly Pro Phe Ala

740 745 750

Gln Phe Ser Glu Gln Asn Asn Asp Asp Phe Leu Thr Asn Gly Gly Thr

755 760 765

His Pro Ala Phe Pro Phe Met Thr Ala His Gly Gly Phe Leu Gln Ala

770 775 780

Val Thr Gln Gly Leu Thr Gly Leu Arg Phe Gly Tyr Val Ile Glu Asp

785 790 795 800

Gly Gln Ile Lys Arg Ala Leu Asp Leu Asp Pro Thr Ala Leu Pro Cys

805 810 815

Leu Pro Asn Gly Val Ile Phe Asp Gly Ile Lys Tyr Asn Asn His Ser

820 825 830

Leu Ser Phe Ala Val Asn Glu Thr Ser Phe Thr Val Lys Asn Asn Gly

835 840 845

Pro Ile Ser Glu Lys Ser Asp Gly Val Val Arg Ile Arg Ile Ala Asp

850 855 860

Arg Asn Pro Ser Arg Gly Ile Tyr Thr Ile Asn Ser Gly Glu Asp Phe

865 870 875 880

Ser Phe Pro Leu Tyr Thr Pro Lys Pro Ser Tyr Pro Thr Ser Ile Ser

885 890 895

Glu Cys Gly Leu Ala Ser Phe Tyr Asn Ile Thr Asp Gly Ala Tyr Gly

900 905 910

Asp Ser Pro Ile Ser Ile Asn Asp Gly Asp Asn Thr Thr Gln Trp Gln

915 920 925

Ala Lys Tyr Asn Asp Thr Thr Gly Lys Ile Leu Val Asp Phe Lys Gln

930 935 940

Phe Lys Asn Val Ser Asn Gly Ile Ile Asn Trp Gly Asp Lys Pro Pro

945 950 955 960

Lys Asn Trp Lys Leu Ser Gln Phe Thr Gly Ser Leu Val Glu Phe Lys

965 970 975

Asp Val Glu Asp Val Leu Ser Gln Val Asp Phe Gly Asn Glu Leu Tyr

980 985 990

Asn Ile Tyr Arg Tyr Glu Asp Glu Asp Tyr Lys Leu Tyr Lys Gln Asp

995 1000 1005

Glu Val Phe Gln Val Val Leu Ser Ser Asn Val Ser Ile Ser Ala

1010 1015 1020

Pro Phe Ile Leu Glu Asp Tyr Asn Thr Ile Glu Leu Pro Lys Arg

1025 1030 1035

Gln Asn Thr Thr Glu Phe Thr Ile Asp Glu Glu Leu Tyr Ser Gln

1040 1045 1050

Phe Leu Leu Ile Glu Ile Glu Gly Ile His Asn Thr Val Pro Ile

1055 1060 1065

Glu Asp Asp Thr Gly Gly Ala Lys Leu Tyr Glu Val Val Phe Phe

1070 1075 1080

<210> 60

<211> 1101

<212> PRT

<213> 法布里德巴利酵母(Debaryomyces fabryi)

<400> 60

Met Glu Tyr Asp Ser Leu Gly Glu Arg Phe Lys His Arg Ala Ile Ser

1 5 10 15

Lys Val Asp His Cys Asn His Phe Ile His Lys Phe Cys Lys Arg Gln

20 25 30

Ile Val Ile Lys Thr Met Ile Val Leu Asn Phe Ile Leu Leu Phe Val

35 40 45

Leu Tyr Leu Tyr His Gln Gly Ile Thr Tyr Ala Leu Pro Phe Ala Ile

50 55 60

Lys Asp Val Asn Ile Asp Asp Ser Phe Asn Asp Ser Thr Val Thr Phe

65 70 75 80

Glu Thr Ile Glu Ser Asn Arg Lys Gln Leu Gln Lys Leu Ile Asn Ser

85 90 95

Arg Glu Asn Lys Glu Ile Phe Leu Gln Leu Gln Asn Ser Gly Ser Ala

100 105 110

Tyr Tyr Asp Pro Thr Thr Asn Thr Val Gly Thr Ala Glu Phe Pro Thr

115 120 125

Tyr Asn Gln Tyr Gln Arg Gln Ala Tyr Val Ser Asn Gly Tyr Ile Gly

130 135 140

Ser Arg Ile Pro Asn Leu Gly Gln Gly Phe Thr Phe Asp Gln Leu Ser

145 150 155 160

Asp Ser Pro Asp Ala Val Glu Asp Asp Leu Ser Asn Gly Trp Pro Leu

165 170 175

Phe Asn Glu Arg Phe Ser Gly Ser Phe Ile Gly Gly Phe Tyr Asp Ile

180 185 190

Gln Lys Asn Thr Thr Glu Thr Asn Phe Pro Glu Leu Ile Lys Lys Gly

195 200 205

Tyr Glu Ser Ile Leu Ser Ala Val Pro Gln Trp Thr Thr Leu Thr Leu

210 215 220

Ser Thr Val Lys Asn Gly Lys Thr Phe Ser Leu Asp Pro Ser Leu Ser

225 230 235 240

Arg Asp Ala Gln Gly Asp Ile Ser Asn Tyr Ala Gln Asn Leu Ser Leu

245 250 255

Ser Asn Gly Ile Val Thr Thr Glu Phe Thr Trp Leu Asp Ser Leu Gln

260 265 270

Val His Ile Glu Val Val Ala His Arg Ser Asn Ile Asn Leu Gly Ile

275 280 285

Val Asn Leu Arg Ile Val Asn Leu Gly Asn Ser Thr Val Asp Leu Lys

290 295 300

Val Glu Asp Lys Leu Glu Phe Ala Ser Thr Gln Arg Cys Gln Leu Thr

305 310 315 320

Glu Val Gly His Asp Asp Glu Gly Ile Phe Ile His Phe Gln Pro Asn

325 330 335

Glu Ile Asp Tyr Val Asn Gly Ala Ile Tyr Ser Thr Leu Lys Tyr Asp

340 345 350

Glu Lys Ser Leu Asn Ser Ile Ser Arg Asp Ser Lys Asn Asp Thr Ser

355 360 365

Thr Gln Glu Leu Asp Ile Ser Val Glu Pro Ser Lys Ser Phe Lys Ile

370 375 380

Ser Lys Met Val Gly Ile Val Ser Ser Asp Leu Asp Pro Glu Lys Tyr

385 390 395 400

Lys Thr Ser Ser Ser Val Asn Asp Phe Ala Lys Gln Val Ala Thr Lys

405 410 415

Gln Ala Asp Ser Val Ser Asp Leu Ile Lys Ser His Lys Thr Glu Trp

420 425 430

Asp Arg Thr Phe Glu Ser Ser Asn Ser Ile Thr Phe Pro Gly Asp Ser

435 440 445

Leu Leu Thr Leu Ala Ser Arg Ala Ser Ile Tyr His Leu Asn Ala Asn

450 455 460

Thr Arg Pro Gly Ala Arg Gly Val Thr Ala Ala Leu Pro Val Gly Gly

465 470 475 480

Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Thr Asp Leu

485 490 495

Trp Met Leu Asn Gly Leu Leu Pro Phe Asn Pro Asp His Ala Lys Ser

500 505 510

Ile Val Asn Tyr Arg Ile His Thr His Glu Gln Ala Lys Lys Asn Val

515 520 525

Pro Asp Asn Lys Gly Gly Ala Val Tyr Ser Trp Thr Ser Gly Arg Phe

530 535 540

Gly Asn Cys Thr Ser Thr Gly Pro Cys Met Asp Tyr Glu Tyr His Ile

545 550 555 560

Asn Ile Ala Val Ala Met Ala Ala Trp Glu Val Tyr Leu Ser Gly Ala

565 570 575

Ala Asp Asp Asp Tyr Leu Asp Ser Val Ala Tyr Pro Leu Ile Asn Asp

580 585 590

Ala Ala Thr Phe Leu Ala Asn Tyr Val Glu Tyr Asn Asp Ser Leu Ala

595 600 605

Gln Tyr Val Thr His Asn Met Thr Asp Pro Asp Glu Tyr Ala Asn His

610 615 620

Val Asp Asn Ala Ala Tyr Thr Asn Ala Gly Ile Ser Leu Leu Met Lys

625 630 635 640

Trp Ala Ile Thr Ile Ser Asn His Leu Gly Lys Ser Val Pro Thr Lys

645 650 655

Tyr Thr Asp Ile Ala Gly Ser Met His Ile Pro Thr Ser Asp Asn Ala

660 665 670

Asp Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Gly Ile

675 680 685

Lys Gln Ala Asp Val Ile Met Met Thr Tyr Pro Leu Gly Asn Glu Leu

690 695 700

Ile Ser Asp Asp Gln Ala Tyr Thr Asn Met Glu Phe Tyr Ser Met Lys

705 710 715 720

Gln Val Ser Tyr Gly Pro Ala Met Thr Phe Pro Ile Phe Ser Ile Val

725 730 735

Ala Ser Asn Leu Ser Pro Ser Gly Cys Ala Ser Gln Ser Tyr Leu His

740 745 750

Lys Ala Val Gln Pro Phe Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu

755 760 765

Gln Asn Asn Asp Asn Phe Leu Thr Asn Gly Gly Thr His Pro Ala Phe

770 775 780

Pro Phe Met Thr Ala His Gly Gly Phe Leu Gln Ala Ile Leu Gln Gly

785 790 795 800

Leu Thr Gly Leu Arg Phe Asp Tyr Asn Ile Asp Asp Thr Asn Lys Leu

805 810 815

Ser Arg Met Leu Thr Leu Asp Pro Ile Ser Leu Pro Cys Leu Gly Asn

820 825 830

Gly Val Gln Phe Asp Ser Ile Lys Tyr Met Asn Lys Thr Leu Ser Leu

835 840 845

Ala Ile Asn Glu Thr Ser Phe Ile Val Lys Asn Asn Gly Pro Ile Thr

850 855 860

Gly Ser Lys Asp Asp Ser Pro Ile Lys Ile Ser Leu Ala Lys Arg Asn

865 870 875 880

Pro Lys Ala Gly Val Tyr Thr Leu Lys Lys Gly Glu Glu Leu Val Phe

885 890 895

Pro Leu Phe Glu Pro Glu Ala Gly Ser Pro Leu Ser Val Ser Glu Cys

900 905 910

Ala Ser Ala Lys Phe Ile Asn Ile Thr Glu Ser Ala Tyr Gly Asp Ala

915 920 925

Thr Val Leu Val Asn Asp Gly Asp Asn Thr Thr His Trp Gln Leu Lys

930 935 940

Tyr Asn Asp Thr Thr Ala Lys Leu Leu Val Asp Leu Lys Gln Pro Arg

945 950 955 960

Asn Ile Thr Ser Gly Val Ile Asn Trp Gly Asp Arg Pro Pro Lys Ser

965 970 975

Trp Ser Leu Leu Ala Tyr Gly Ser His Glu Gln Ser Lys Ile Lys Asp

980 985 990

Leu Asn Asp Asn Ile Asp Phe Leu Ser Lys Val Asn Phe Gly Asn Asn

995 1000 1005

Leu Tyr Lys Lys Tyr Glu Phe Met Asp Val Asp Met Val Tyr Asp

1010 1015 1020

Gln Asp Asp Val Phe Thr Thr Ile Ile Ser Glu Ser Val Asp Ile

1025 1030 1035

Ser Ala Pro Tyr Asp Pro Lys Glu Ser Ala Gln Ile Glu Ile Pro

1040 1045 1050

Leu Arg His Asn Thr Thr Ser Phe Ala Val Glu Gln Gly Leu Asn

1055 1060 1065

Ala Arg Phe Leu Leu Ile Glu Val Thr Asp Ile His Asp Thr Glu

1070 1075 1080

Pro Ile Asp Asp Glu Thr Gly Gly Ala Lys Leu Tyr Glu Val Glu

1085 1090 1095

Phe Phe Glu

1100

<210> 61

<211> 1049

<212> PRT

<213> 坦扎瓦丝假丝酵母(Candida tanzawaensis)

<400> 61

Met Met Ile Val Ala Asn Leu Ala Phe Leu Leu Val Leu Gln Leu Thr

1 5 10 15

Trp Asp Val Leu Trp Ala His Pro Leu Thr Val Pro Lys Leu Phe Ala

20 25 30

Ala Ser Asn Leu Ser Ala Asp Thr Val His Thr Asp Arg Asp Ala Leu

35 40 45

Phe Ala Leu Ile Asn Ser Thr Ala Asn Lys Gln Val Phe Ser Gln Leu

50 55 60

Lys Tyr Ser Ser Ser Ala Tyr Tyr Asp Leu Asp Ala Asn Ile Val Gly

65 70 75 80

Ser Ala Glu Phe Ala Pro Asn Asn Gln Tyr Gln Arg Gln Pro Tyr Val

85 90 95

Ala Asn Gly Tyr Ile Gly Ala Arg Ile Pro Asn Leu Gly Gln Gly Phe

100 105 110

Thr Tyr Asp Gln Leu Ser Thr Asp Ala Asp Ala Thr Asp Ala Asp Leu

115 120 125

Leu Asn Gly Trp Pro Leu Tyr Asn Lys Arg Tyr Ala Gly Ala Phe Ile

130 135 140

Ala Gly Phe Tyr Asp Ile Gln Ala Asn Thr Thr Glu Thr Asn Phe Pro

145 150 155 160

Glu Leu Glu Lys Asn Gly Tyr Glu Ser Ile Ile Ala Ala Val Pro Gln

165 170 175

Trp Thr Ala Leu Glu Leu Ser Thr Thr Val Asp Gly Val Asn Tyr Thr

180 185 190

Leu Asn Pro Ala Leu Glu Ser His Leu Gln Gly Ser Ile Ser Asn Tyr

195 200 205

Ala Gln Asn Leu Ser Leu Ser Asp Gly Ile Val Ser Thr Glu Phe Thr

210 215 220

Trp Leu Asp Ser Val Gln Val Lys Ile Glu Ile Leu Ala His Arg His

225 230 235 240

His Val Asn Leu Gly Val Val His Leu Glu Leu Ala Asn Ile Gly Ser

245 250 255

Ser Ala Leu Asp Ile Lys Val Arg Asp Val Leu Asp Phe Ala Thr Ala

260 265 270

Gln Arg Ser Gln Leu Ala Ser Val Asp His Asp Gln Asp Gly Ile Tyr

275 280 285

Ile Thr Phe Gln Pro Asn Glu Ile Asp Tyr Val Asn Gly Ala Ile Tyr

290 295 300

Ser Thr Leu Leu Ser Asp His Pro Pro Asn Phe Glu Gln Ala Thr Asn

305 310 315 320

Gly Thr Val Ala Gln Ser Leu Asp Ile Gln Leu Gln Pro Gly Ser Thr

325 330 335

Phe Lys Val Thr Lys Tyr Ala Gly Ile Val Ser Ser Asp Leu Asp Pro

340 345 350

Ala Asn Leu Lys Ser Glu Lys Asp Val Leu Ser Leu Ala Arg Arg Val

355 360 365

Ser Gln Ser Phe Thr Asp Ile Lys Glu Val Phe Glu Thr His Lys Thr

370 375 380

Ala Trp Ala Gln Thr Leu Glu Ser Thr Pro Ala Val Thr Phe Pro Asn

385 390 395 400

Asp Leu Leu Leu Thr Ile Ala Ala Arg Ala Ser Ile Tyr His Leu Ala

405 410 415

Ala Asn Thr Arg Thr Asp Ala Glu Gly Val Thr Ala Ala Leu Pro Val

420 425 430

Val Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Thr

435 440 445

Asp Leu Trp Met Phe Asn Gly Leu Leu Pro Phe Leu Pro Ser His Ala

450 455 460

Lys Ser Leu Ile Asn Tyr Arg Ile His Thr His Glu Gln Ala Lys Lys

465 470 475 480

Asn Val Pro Ala Gly Tyr Glu Gly Ala Ala Tyr Pro Trp Thr Ser Gly

485 490 495

Arg Phe Gly Asn Cys Thr Ser Thr Gly Pro Cys Val Asp Tyr Glu Tyr

500 505 510

His Ile Asn Met Ala Val Ala Ser Ser Ala Trp Asn Leu Tyr Leu Ser

515 520 525

Gly Glu Val Gly Glu Asp Phe Leu Glu Ser Val Ala Tyr Pro Leu Ile

530 535 540

Lys Asp Ala Ala Thr Phe Phe Ser Glu Tyr Val Val Lys Leu Asp Glu

545 550 555 560

Ser Thr Gly Lys Tyr Val Thr His Asn Leu Thr Asp Pro Asp Glu Tyr

565 570 575

Ala Asn His Val Asp Asn Gly Ala Tyr Thr Asn Ala Gly Ile Ser Leu

580 585 590

Val Met Lys Trp Ala Ile Asp Val Ser Asn His Leu Gly Lys Glu Val

595 600 605

Pro Ser Gln Phe Met Asp Ile Ser Asp Asn Val Leu Met Pro Thr Ser

610 615 620

Asn Asn Ser Asp Gln Ile Thr Leu Glu Tyr Ser Gly Met Asn Ala Thr

625 630 635 640

Val Gly Ile Lys Gln Ala Asp Val Ile Met Val Asn Tyr Pro Leu Glu

645 650 655

Asn Glu Leu Ile Thr Pro Glu Gln Ala Leu Lys Asn Met Glu Phe Tyr

660 665 670

Ser Lys Lys Gln Val Ser Tyr Gly Pro Ala Met Thr Phe Pro Ile Phe

675 680 685

Ser Ile Val Ala Ser Asn Val Ser Pro Ser Gly Cys Ala Ser Gln Ser

690 695 700

Tyr Leu Arg Lys Ala Val Gln Pro Phe Leu Arg Gly Pro Phe Ala Gln

705 710 715 720

Phe Ser Glu Gln Asn Asn Asp Asp Phe Leu Thr Asn Gly Gly Thr His

725 730 735

Pro Ala Phe Pro Phe Met Thr Ala His Gly Gly Phe Leu Gln Ala Ile

740 745 750

Met Gln Gly Leu Thr Gly Leu Arg Tyr Asp Phe Ala Val Lys His Asn

755 760 765

Lys Ile Gln Arg Val Leu Lys Leu Asp Pro Ile Ala Ile Pro Cys Leu

770 775 780

Gly Gly Gly Val Phe Tyr Asp Gly Ile His Phe Asp Asn His Thr Leu

785 790 795 800

Thr Met Ser Ile Asn Glu Thr Tyr Phe Thr Val Lys Asn Asn Gly Pro

805 810 815

Ala Asn Glu Phe Ala Ser Glu Ser Ile Glu Ile Arg Val Gly Glu Arg

820 825 830

Asn Lys Glu Ala Gly Gln His Thr Leu Lys Asn Gly Glu Thr Leu Thr

835 840 845

Ile Pro Ile Phe Thr Pro Gly Glu Ser Tyr Lys Ala Ser Ile Thr Glu

850 855 860

Cys Ala Leu Ser Asp Phe Val Asn Ile Thr Ala Gly Ala Tyr Gly Asp

865 870 875 880

Ala Thr Val Leu Met Asn Asp Gly Asp Asn Thr Thr Asn Trp Gln Val

885 890 895

Ala Ser Asn Thr Ser Val Gly Lys Val Leu Ile Asp Leu Lys Glu Tyr

900 905 910

Lys Asn Val Thr Lys Gly Phe Phe Asn Trp Gly Asp Lys Pro Pro Lys

915 920 925

Gln Val Lys Leu Ser Ala Tyr Asn Ser Leu His Asp Leu Gln Asn Thr

930 935 940

Thr Ala Phe Leu Ser Lys Ile Asp Phe Gly Asn Glu Val Tyr Thr Asn

945 950 955 960

Tyr Ala Tyr Ala Asn Pro Ser Gly Glu Leu Ile Lys Gln Glu Glu Val

965 970 975

Phe Glu Ser Phe Leu Glu Glu Ser Val Asp Val Thr Val Pro Phe Thr

980 985 990

Met Asp Asp Phe Leu Ser Leu Thr Val Pro Val Val Phe Asn Thr Thr

995 1000 1005

Thr Phe Glu Leu Pro Asn Pro Val Phe Thr Arg Phe Val Leu Leu

1010 1015 1020

Glu Leu Gln Gly Ile His Asp Thr Glu Pro Ile Asp Leu Asp Thr

1025 1030 1035

Ser Gly Ala Lys Val Tyr Glu Ala Val Leu Tyr

1040 1045

<210> 62

<211> 1147

<212> PRT

<213> 多布克鲁维酵母(Kluyveromyces dobzhanskii)

<400> 62

Met Met Leu Ile Leu Pro Leu Leu Leu Phe Ser Val Thr Ile Leu Ala

1 5 10 15

Pro Val Tyr Phe Tyr Leu Ser Lys Pro Met Pro Leu Gln His Pro Leu

20 25 30

Phe Gly Ser Ile Val Cys Val Asp Glu Glu Leu Tyr Cys Pro Glu Ser

35 40 45

Lys Arg Lys Ala Ser Glu Lys Met Tyr Ser Leu Leu Lys Asp Ser Asp

50 55 60

His Thr Phe Tyr Asp Glu Glu Gln Gln Val Leu Gly Asn Leu Leu Pro

65 70 75 80

Ser Glu Asn Thr Phe Ser Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile

85 90 95

Gly Ser Arg Ile Pro Asn Leu Gly Phe Gly Tyr Ala Leu Asp Thr Met

100 105 110

Asn Val Trp Val Asn Asp Ser Ser Ile Pro Gly Ala Leu Asp Asn Gly

115 120 125

Trp Pro Leu Arg Asn Arg Arg Phe Ala Gly Ala Phe Val Ser Asp Phe

130 135 140

Tyr Cys Leu Gln Glu Lys Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp

145 150 155 160

Asp Asn Gly Tyr Ser Thr Val Ile Ser Thr Ile Pro Gln Trp Thr Asp

165 170 175

Leu Ser Ile Ile Arg His Thr Asp Leu Gly Glu Val Glu Tyr Ile Asn

180 185 190

Pro Thr Asp Val Asn Leu Asp Lys Ile Ser Asn Tyr Met Gln Asn Leu

195 200 205

Ser Leu Gln Asp Gly Ile Val Thr Thr Ser Phe Val Tyr Asp Asn Ser

210 215 220

Leu Leu Val Thr Thr Lys Val Ile Ala His Arg Lys Ile Tyr Pro Leu

225 230 235 240

Gly Ile Val Thr Leu Glu Leu Ser Ser Val Gly Asn Asn Glu Phe Ser

245 250 255

Asp Gly Leu His Ser Lys Ser Val Glu Leu Glu Ile Cys Asp Thr Leu

260 265 270

Asn Phe Ser Thr Ser His Arg Thr Val Leu Ala Asp His Gly Tyr Asp

275 280 285

Arg Lys Asn Asn Gly Ile Phe Met Val Val Glu Pro Glu Asn Val Pro

290 295 300

Tyr Ser Asn Ala Ser Leu Phe Ser Tyr Phe Asp Ile Ala Ser Arg Asp

305 310 315 320

Asn Leu Thr Phe Thr Lys Ser Pro Asn Ser Ile Asn Gln Cys Thr Lys

325 330 335

His Val Leu Thr Pro Asn Thr Thr Phe Val Ala His Lys Tyr Val Gly

340 345 350

Ile Ile Ser Ser Glu Tyr Asp Asn Gln Ala Ser Thr Val Val Thr Asn

355 360 365

Lys Thr Asp Asp Asn Leu Tyr Glu Met Ser Asn Leu Glu Arg Ala Ile

370 375 380

Ser Ile Val Ser Ser Asn Gln Gly Asn Tyr Glu Ser Leu Ile Gln Leu

385 390 395 400

His Arg Glu Thr Trp Glu Lys Ile Tyr Lys Asp Ala Ser Ile Glu Ile

405 410 415

Pro Ser Asp Arg Leu Leu Glu Met Thr Ala Arg Ser Ser Ile Tyr His

420 425 430

Leu Leu Ala Asn Thr Arg Ser His Asn Ile Ser Lys Asp Arg Gly Leu

435 440 445

Pro Leu Gly Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val

450 455 460

Phe Trp Asp Ser Asp Leu Trp Ile Leu Pro Ser Leu Leu Pro Phe Phe

465 470 475 480

Pro Glu Thr Ala Lys Gln Ile Asn Asn Tyr Arg Asn Ala Ser Leu Ser

485 490 495

Gln Ala Lys Leu Asn Ala Gln Gln Tyr Gly Tyr Glu Gly Ala Val Tyr

500 505 510

Pro Trp Thr Ser Gly Arg Phe Ala Asn Cys Thr Ser Thr Gly Pro Cys

515 520 525

Val Asp Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ser Ser Phe

530 535 540

Ala Ile Tyr Met Asn Gly Gly Val Asp Asp Glu Glu Ser Glu Glu Tyr

545 550 555 560

Leu Arg Tyr Thr Thr Trp Pro Phe Val Glu Asn Ala Ala Lys Leu Phe

565 570 575

Thr Gln Tyr Val Lys Trp Asn Asp Ser Met Gln Gln Tyr Thr Thr His

580 585 590

Asn Leu Thr Asp Pro Asp Glu Phe Ala Asn Phe Ile Asp Asn Ala Ala

595 600 605

Phe Thr Asn Ala Gly Ile Gln Thr Ile Met Val Trp Ala Thr Asp Ile

610 615 620

Ala Arg His Leu Gly Ile Glu Pro Asp Pro Lys Trp Leu Asn Ile Ala

625 630 635 640

Asp Asn Ile His Ile Pro Ile Ser Asp Ser Asn Ile Thr Leu Glu Tyr

645 650 655

Ser Gly Met Asn Ser Ser Ile Asp Ile Lys Gln Ala Asp Val Val Leu

660 665 670

Met Ile Tyr Pro Leu Ser Tyr Ile Thr Asp Gln Ser Ile Leu Asn Asn

675 680 685

Ala Ile Lys Asn Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly

690 695 700

Pro Ala Met Thr Tyr Pro Val Phe Val Ala Gly Ala Ala Ser Leu Leu

705 710 715 720

Asn His Gly Cys Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Val Pro

725 730 735

Tyr Leu Arg Ser Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn

740 745 750

Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala

755 760 765

Asn Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg

770 775 780

Tyr Ser Tyr Glu Phe Asp Glu Glu Ala Glu Lys Ile Asn Arg Leu Leu

785 790 795 800

Lys Phe Asn Pro Ile Gln Leu Pro Leu Leu Pro Gly Gly Ile Arg Ile

805 810 815

Asn Asn Phe Lys Tyr Met Gly Gln Val Leu Asp Val Leu Ile Thr Asp

820 825 830

Thr Glu Gly Ile Ile Ile His Lys Lys Gly Asn Lys Asp Ile Lys Ile

835 840 845

Lys Val Pro Asp Arg Ala Leu Ile Pro Asp Ile Glu Ile Glu Asn Pro

850 855 860

Ser Glu Ala Asp Pro Ile Lys Gln Ile Met His Gly Arg Arg Ser Ser

865 870 875 880

His Asn Asn Lys Asn Tyr Phe Thr Ile Lys Pro Gly Asp Val Phe Lys

885 890 895

Thr Pro Leu Tyr Ile Pro Lys Lys Asn Ile Glu Gly Asn Leu Val Glu

900 905 910

Ala Lys Gln Ile Thr Asn Leu Thr Glu Gly Val Pro Gly Asp Val Ala

915 920 925

Val Ser Ile Leu Asp Gly Asn Asn Phe Thr His Trp Gln Pro Ala Tyr

930 935 940

Lys Lys Leu Pro Ala Lys Leu Leu Ile Asp Leu Gly Asn Asn Thr Glu

945 950 955 960

Asp Asp Ile Val Ala Gly Lys Ile Leu Trp Gly Ser Arg Pro Ala Lys

965 970 975

Ser Phe Ser Leu Ser Ile Leu Pro Gln Thr Glu Glu Ile Ala Gln Asn

980 985 990

Leu Thr His Ile Leu Ser Asn Val Asn Gln Tyr Cys Asn Glu Ser Ser

995 1000 1005

Asp Thr Cys Glu Arg Lys Leu Gln Asn Pro Lys Glu Asp Phe Asp

1010 1015 1020

Ala Ala Ile Ile Asp Ile Phe Asp Trp Tyr Gly Ile Asp Leu Asp

1025 1030 1035

Ala Ile Arg Lys Lys His Pro Glu Leu Gly Glu Ile Lys Thr Lys

1040 1045 1050

Phe Val Lys Leu Leu Asp Asn Tyr Thr Val Thr Pro Ser Glu Pro

1055 1060 1065

Tyr Pro Trp Arg Val Tyr Asn Glu Ser Lys Ile Val Leu Leu Pro

1070 1075 1080

Gly Asn Glu Thr Asp Phe Thr Ile Asp Tyr Ser Lys Val Pro Asp

1085 1090 1095

Leu Asn Lys Asp Asn Glu Asp Ile Asp Phe Arg Ser Asp Glu Thr

1100 1105 1110

Asn Trp Lys Lys Pro Arg Phe Ile Val Leu Thr Val His Asp Thr

1115 1120 1125

Tyr Asp Asn Asp Asp Asn Glu Lys Gly Ala Thr Ile Lys Glu Phe

1130 1135 1140

Ala Leu Phe Ser

1145

<210> 63

<211> 1157

<212> PRT

<213> 马克斯克鲁维酵母(Kluyveromyces marxianus)

<400> 63

Met Ile Ile Ile Pro Leu Val Val Leu Val Phe Thr Val Leu Ala Pro

1 5 10 15

Val Tyr Phe Tyr Val Thr Lys Pro Glu Ser Ser Thr His Ser Leu Phe

20 25 30

Pro Glu Leu Ala Pro Ala Arg Ile Ser Trp Pro Phe Ala Gly Thr Cys

35 40 45

Ala Ser Ser Ser Gly Gly Glu Glu Asp Pro Leu Tyr Cys Pro Asp Ala

50 55 60

Tyr Arg Lys Ala Ser Glu Lys Met Tyr Asp Leu Leu Lys Asp Asn Glu

65 70 75 80

Tyr Ala Phe Tyr Asp Glu Thr Ser Glu Thr Leu Gly Asn Leu Leu Leu

85 90 95

Ser Glu Asn Thr Phe Ser Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile

100 105 110

Gly Ser Arg Ile Pro Asn Val Gly Phe Gly Phe Ala Tyr Asp Ala Ile

115 120 125

Asn Ile Trp Val Asn Asp Ser Ala Ile Pro Gly Ala Leu Asn Asn Gly

130 135 140

Trp Pro Leu Arg Asn Gln Arg Tyr Ala Gly Ser Phe Val Ser Asp Phe

145 150 155 160

Tyr Ser Leu Gln Glu Lys Leu Asn Ser Thr Asn Phe Ala Glu Leu Asp

165 170 175

Lys Asp Gly Tyr Ser Thr Val Ile Ser Ser Ile Pro Asp Trp Thr Asp

180 185 190

Leu Ser Ile Met Ile His Arg Gly Pro Gly Glu Asn Asn Val Glu Tyr

195 200 205

Ile Asn Pro Thr Asp Val Lys Leu Asp Lys Ile Thr Asp Tyr Met Gln

210 215 220

Asn Leu Ser Met Arg Asp Gly Ile Val Thr Thr Lys Phe Val Tyr Asp

225 230 235 240

Asn Asn Leu Phe Val Thr Thr Arg Thr Leu Ala His Arg Ser Ile Tyr

245 250 255

Pro Leu Gly Ile Val Asp Met Glu Ile Glu Leu Leu Pro Gln Ala Thr

260 265 270

Glu Asn Gly Leu His Glu Ala Ser Val Glu Leu Glu Ile Cys Asp Thr

275 280 285

Phe Asn Phe Thr Thr Ser His Arg Thr Val Leu Ala Asp Phe Gly His

290 295 300

Asp Lys Lys Asn Glu Gly Ile Tyr Met Ile Val Glu Pro Glu Asn Val

305 310 315 320

Pro Tyr Ser Asn Ala Ser Met Phe Ser Tyr Phe Asp Ile Pro Ser Arg

325 330 335

Asp Glu Tyr Thr Val Ala Lys Thr Asn Asp Ser Val Ser Gln Cys Thr

340 345 350

Arg Arg Val Leu Thr Thr Asp Ser Arg Glu Asn Ser Thr Phe Ile Val

355 360 365

Arg Lys Phe Thr Gly Ile Val Ser Ser Glu Tyr Asp Asn Asn Asn Pro

370 375 380

Glu His Met Ser Asn Leu Glu Arg Ala Thr Ala Val Val Met Glu Asn

385 390 395 400

Lys Gly Asp Tyr Lys Asn Leu Leu Lys Met His Arg Asp His Trp Lys

405 410 415

Arg Leu Tyr Ala Asp Ala Ser Ile Glu Ile Pro Ser Asp Gly Leu Leu

420 425 430

Glu Met Thr Ala Lys Ser Ser Ile Tyr His Leu Leu Ala Asn Ser Arg

435 440 445

Ser His Asn Val Ser Gln Ser Arg Gly Leu Pro Val Pro Pro Ser Gly

450 455 460

Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Ala Asp Val

465 470 475 480

Trp Met Leu Pro Ala Leu Leu Pro Phe Phe Pro Glu Ile Ala Lys Gln

485 490 495

Met Ser Ala Tyr Arg Asn Ala Ser Leu Ala Gln Ala Lys Glu Asn Ala

500 505 510

Lys Lys Tyr Gly Leu Gln Gly Ala Ile Phe Pro Trp Thr Ser Gly Arg

515 520 525

Phe Ala Asn Cys Thr Ser Thr Gly Pro Cys Val Asp Tyr Glu Tyr His

530 535 540

Ile Asn Val Asp Ile Ala Leu Ser Ser Leu Tyr Ile Tyr Met Ser Gly

545 550 555 560

Glu Glu Asp Glu Glu Lys Ser Glu Glu Tyr Leu Arg Tyr Thr Thr Trp

565 570 575

Pro Phe Ile Glu Asn Ala Ala Lys Met Phe Thr Asp Tyr Val Lys Trp

580 585 590

Asn Asp Thr Leu Gln Gln Tyr Thr Thr His Asn Leu Thr Asp Pro Asp

595 600 605

Glu Phe Ala Asn His Val Asp Asn Gly Ala Phe Thr Asn Ala Gly Ile

610 615 620

Lys Ser Ile Met Gly Trp Ala His Asp Ile Ala Asn His Leu Gly Leu

625 630 635 640

Asp Pro Asp Pro Lys Trp Thr Glu Ile Ala Glu Lys Ile His Ile Pro

645 650 655

Ile Ser Asp Thr Asn Ile Thr Leu Glu Tyr Thr Gly Met Asn Ser Ser

660 665 670

Val Asp Ile Lys Gln Ala Asp Val Val Leu Met Thr Tyr Pro Leu Gly

675 680 685

Tyr Phe Thr Glu Thr Ser Gln Pro Arg Asn Ala Ile Lys Asp Ile Tyr

690 695 700

Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro

705 710 715 720

Val Phe Val Ala Ala Ser Ala Ser Leu Leu Asn Ser Gly Ser Ser Ser

725 730 735

Gln Ser Tyr Leu Tyr Lys Ser Val Val Pro Tyr Leu Arg Ser Pro Phe

740 745 750

Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu

755 760 765

Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Tyr Leu Gln

770 775 780

Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr Glu Val Asp

785 790 795 800

Lys Asp Thr Gly Lys Met His Arg Leu Leu Lys Phe Asn Pro Ile Ser

805 810 815

Leu Pro Met Phe Pro Gly Gly Ile Arg Ile Asn Asn Phe Lys Tyr Met

820 825 830

Gly Gln Val Leu Asp Ile Leu Leu Thr Asp Asn Glu Gly Ile Ile Lys

835 840 845

His Lys Lys Gly Asn Lys Ser Ile Leu Ile Lys Ile Pro Asp Arg Gly

850 855 860

Asp Ile Pro Asp Val Lys Pro Asp Glu Tyr Thr Gln Ile Asn Gly Thr

865 870 875 880

Ser Val Asn Val Lys Arg Ala Val Pro Ser Gly Glu Ser Tyr His Thr

885 890 895

Ile Glu Pro Gly Thr Val Phe Lys Thr Pro Leu Tyr Asn Pro Lys Arg

900 905 910

Asn Met Ala Asn Asn Ile Val Glu Ser Lys Arg Ala Thr Asn Ile Thr

915 920 925

Val Gly Val Pro Gly Asp Val Ala Val Ser Ala Ile Asp Gly Asn Asn

930 935 940

Tyr Thr His Trp Gln Pro Ala Asn Lys Lys Gln Pro Gly Arg Ile Leu

945 950 955 960

Ile Asp Met Gly Asn Gly Thr Ala Asn Glu Ile Lys Ser Gly Lys Ile

965 970 975

Leu Trp Gly Asn Arg Pro Ala Lys Ser Phe Ser Leu Ser Ile Leu Pro

980 985 990

Gln Phe Asp Gln Ile Thr Gln Asn Met Thr Ser Val Leu Ser Gln Pro

995 1000 1005

Ser Ser His Asn Cys Ser Asn Asp Asp Gly Trp Asp Ser Asn Cys

1010 1015 1020

Lys Tyr Gln Glu Glu Glu Glu Asn Ile Asp Ala Ala Ile Lys Asp

1025 1030 1035

Val Phe Glu Trp Tyr Gly Met Asp Leu Gln Ser Val Ile Glu Asn

1040 1045 1050

Tyr Pro Glu Leu Ser Asn Val Ser Met Gly Phe Ile Lys Leu Val

1055 1060 1065

Asp His Tyr Asn Val Thr Pro Ser Tyr Pro Trp Lys Asn Val Asn

1070 1075 1080

Ser Thr Arg Ile Glu Leu Thr Leu Gly Asn Glu Thr Asn Phe Val

1085 1090 1095

Val Asp Tyr Ser Lys Val Pro Glu Leu Asn Leu Asn Asn Asn Leu

1100 1105 1110

Gly Val Asp Leu Gln Ser Lys Asp Thr Arg Trp Arg Lys Pro Arg

1115 1120 1125

Phe Val Val Leu Thr Val Phe Asp Thr Tyr Asp Asp Asp Asp Glu

1130 1135 1140

Val Lys Gly Ala Thr Ile Lys Glu Leu Ser Leu Phe Asp Asn

1145 1150 1155

<210> 64

<211> 1169

<212> PRT

<213> 鲁氏接合酵母(Zygosaccharomyces rouxii)

<400> 64

Met Met Lys Ile Asp Leu Asn Ala Asp Pro Gly Asp Ile Ser Gln Thr

1 5 10 15

Pro Gln Glu Arg Glu Tyr Arg Asp Ser Ser Glu Ser Thr Trp Lys Lys

20 25 30

Phe Ile Ser Ile Thr Met Ala Leu Leu Ala Ile Phe Gly Leu Leu Leu

35 40 45

Phe Phe Gly Trp Gln Thr Val Asn Gly Gln Ser Val Leu Pro Ala Ala

50 55 60

Gln Gly Pro Gly Ile Ile Val Lys Ala Asn Arg Asp His Asp Leu Gln

65 70 75 80

Thr Asn Asn Glu Val Arg Lys Ala Ser Lys Lys Leu Tyr Glu Leu Leu

85 90 95

Ala Asp Thr Glu Gly Ala Tyr Tyr Asp Ala Asp Ala Met Val Leu Gly

100 105 110

Ser Thr Val Phe Ser Pro His Thr Tyr Ser Arg Gln Pro Tyr Val Ala

115 120 125

Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Val Gly Phe Gly Tyr Ala

130 135 140

Glu Asp Val Trp Asn Ile Trp Ala Asn Ala Asp Asp Thr Val Pro Gly

145 150 155 160

Ala Leu Glu Asn Gly Trp Pro Leu Arg Asn Arg Arg Phe Ala Gly Ala

165 170 175

Phe Val Ser Asp Phe Tyr Cys Leu Gln Glu Ser Leu Asn Ser Thr Asn

180 185 190

Phe Pro Glu Leu Asp Lys Asn Gly Tyr Thr Thr Val Ile Ser Ser Ile

195 200 205

Pro Gln Trp Thr Gln Leu Gln Phe Ser Leu Asn Asn Glu Lys Trp Phe

210 215 220

Asp Pro Gln Thr Val Asp Pro Lys Asp Val Ala Asn Tyr Thr Gln Glu

225 230 235 240

Leu Ser Met Gln Asn Gly Ile Val Thr Thr Gln Leu Asp Trp Leu Asp

245 250 255

Gly Leu Leu His Val Glu Thr Gln Val Leu Ala His Lys Lys Ile Tyr

260 265 270

Thr Leu Gly Ile Val Ser Met Lys Val Ser Leu Asn Val Asp Arg Leu

275 280 285

Pro Gln Gly Glu Ser Ser Val Glu Leu Asn Phe Arg Asp Val Leu Asp

290 295 300

Phe Asn Thr Ser His Arg Thr Val Leu Ala Asp Lys Gly Tyr Asp Glu

305 310 315 320

Gly Ile Tyr Met Val Val Glu Pro Glu Asn Val Pro Tyr Ser Asn Thr

325 330 335

Ser Met Phe Ser Lys Cys Glu Ile Asp Asn Asp Gly Val Lys Phe Asn

340 345 350

Glu Phe Gly Ser Asp Asp Glu Thr Val Ser Gln Asn Ala Ser Val Ile

355 360 365

Leu Thr Lys Asp Asn Pro Gln Val Thr Ile His Lys Tyr Val Gly Ile

370 375 380

Phe Ser Thr Glu Tyr Tyr Asn Gly Pro Thr Leu Asn Arg Ala Lys Glu

385 390 395 400

Val Ala Val Thr Ser Tyr Gly Asn Tyr Asp Lys Leu Thr Lys Ala His

405 410 415

Asn Glu Ala Trp Lys Asn Ile Tyr Asp Asn Ala Phe Ile Glu Ile Pro

420 425 430

Ser Asp Glu Val Leu Glu Met Thr Ala Arg Ser Ser Leu Tyr His Leu

435 440 445

Leu Ala Asn Thr Arg Thr Tyr Asn Val Ser Ala Glu Lys Gly Leu Pro

450 455 460

Val Pro Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe

465 470 475 480

Trp Asp Ser Asp Phe Trp Ile Gln Pro Gly Ile Leu Pro Phe Phe Pro

485 490 495

Asp Ile Ala Lys Asn Ile Asn Asn Tyr Arg Asn Ala Thr His Glu Gln

500 505 510

Ala Gln Leu Asn Ala Lys Gln Tyr Gly Tyr Pro Gly Ala Leu Tyr Pro

515 520 525

Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Val

530 535 540

Asp Tyr Glu Tyr His Ile Asn Val Asp Val Cys Leu Ala Ser Phe Ser

545 550 555 560

Ile Tyr Leu Asn Gly Ala Lys Gly Ile Asp Asp Asp Tyr Leu Arg Tyr

565 570 575

Thr Thr Trp Pro Met Val His Asp Ala Ala Glu Phe Phe Thr Ala Phe

580 585 590

Val Lys Tyr Asn Ser Thr Ile Asp Ser Tyr Glu Thr His Asn Leu Thr

595 600 605

Asp Pro Asp Glu Phe Ala Asn His Val Asp Asn Gly Ala Phe Thr Asn

610 615 620

Ala Gly Ile Lys Leu Leu Leu Lys Trp Ala Thr Asp Ile Gly Tyr His

625 630 635 640

Leu Lys Glu Pro Val Asp Pro Arg Trp Met Glu Ile Ser Glu Lys Ile

645 650 655

His Ile Pro Gln Ser Asp Ser Asn Ile Thr Leu Glu Tyr Ser Gly Met

660 665 670

Asn Ser Ser Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val Tyr

675 680 685

Pro Leu Ala Phe Ile Thr Asp Glu Ser Ile Leu Asn Asn Ala Ile Lys

690 695 700

Asp Val Tyr Tyr Tyr Ser Glu His Gln Ser Ser Thr Gly Pro Ala Met

705 710 715 720

Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Gly Ile Leu Asn His Gly

725 730 735

Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Leu Leu Pro Tyr Leu Arg

740 745 750

Ala Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr

755 760 765

Asn Gly Lys Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly

770 775 780

Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr

785 790 795 800

Glu Val Glu Pro Gly Ser Lys Lys Ile Gln Arg Ile Leu Lys Phe Asn

805 810 815

Pro Ile Glu Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn Phe

820 825 830

Lys Tyr Leu Gly Gln Val Leu Asp Ile Ile Ile Asp Asp Arg Asn Gly

835 840 845

Thr Ile Val His Lys Ser Gly Asp Gln Pro Ile Arg Ile Lys Val Pro

850 855 860

Asp Arg Thr Leu Ile His Asp Arg Asp Ile Thr Pro Tyr His Gly Asn

865 870 875 880

Gly Ser Ala Gly Met Glu Ser Ile Lys Ile Asp Asn Thr Thr His Gly

885 890 895

Thr Ser Ala His Tyr Tyr Thr Ile Asn Pro Gly Glu Glu Leu Ile Leu

900 905 910

Pro Leu Phe His Met Asp Gln Asn Val Glu Gly Asn Leu Val Glu Ser

915 920 925

Lys Gln Ile Thr Asn Leu Thr Ala Gly Val Pro Gly Asp Val Ser Ile

930 935 940

Ser Thr Leu Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Ala Val Lys

945 950 955 960

Ser Leu Pro Gly Lys Leu Leu Ile Asp Leu Gly Ala Asn Asn Ser Gln

965 970 975

Val Ile Asn Lys Gly Met Ile Leu Trp Gly Gln Arg Pro Ala Lys Asn

980 985 990

Phe Thr Ile Ser Ile Leu Pro His Thr Asp Val Ile Gly Gln Ile Leu

995 1000 1005

Ser Asp Leu Thr Thr Ile Leu Glu Asn Ala Asn Val Gly Asp Glu

1010 1015 1020

Ser Val Leu Glu Ala Leu Glu Asp Ile Gly Gly Ser Ser Pro Leu

1025 1030 1035

Leu Ser His Thr Gly Asp Thr Asp Val Lys Asp Leu Leu Ser Trp

1040 1045 1050

Lys Ala Glu Asp Val Asp Asp Ile Leu Leu Gly Ile Ser Gly Glu

1055 1060 1065

Ser Asn Val Ile Arg Gly Lys Phe Val Pro Ile Leu Lys Asp Tyr

1070 1075 1080

Lys Val Glu Pro Ser Glu Pro Tyr Phe Glu Glu Ile Tyr Ser Lys

1085 1090 1095

Ser Ile Ile Glu Ile Leu Pro Ser Asn Lys Thr Glu Phe Ala Ile

1100 1105 1110

Asp Tyr Glu Lys Val Gln His Asn Ser Thr Ser Cys Ser His Lys

1115 1120 1125

Asp Pro Ser Asp Asn Cys Thr Asp Trp Thr Lys Thr Arg Phe Ile

1130 1135 1140

Met Val Ser Ile Glu Gly Ser Tyr Asp Asp Asp Asp Asp Glu Arg

1145 1150 1155

Gly Gly Thr Ile Lys Glu Ile Val Leu Met Asn

1160 1165

<210> 65

<211> 1278

<212> PRT

<213> 达氏诺莫维酵母(Naumovozyma dairenensis)

<400> 65

Met Ser Phe Leu Lys Arg Ile Phe Phe Lys Glu Arg Pro Pro Ile Ile

1 5 10 15

Thr Ala Thr Glu Val Gly Tyr Ile Ser Ser Asn Cys Asp Val Glu Thr

20 25 30

Tyr Pro Leu Thr Ser Thr Glu Thr His Ile Asp Asn Asn Ile Asn Ile

35 40 45

Asp Thr Asn Asn Thr Glu Leu Tyr Thr Asn Asp Ser Asn Asn Asn Asn

50 55 60

Asn Tyr Asn Asn Asp Asn Ile Pro Thr Ile Gly Glu Gln Arg Arg Gln

65 70 75 80

Leu Asn Lys Thr Phe Asn Lys Ala Thr Ile Thr Lys Tyr Leu Leu Ala

85 90 95

Phe Ile Met Ile Ala Gly Leu Ile Ala Leu Ala Ala Val Ala Ser Thr

100 105 110

Ile Asp Leu Ser Ser Ala Phe Ser Lys Gly Lys Ser Ser Gln Ser Met

115 120 125

Asp Glu Asp Ile Asn Asn Ile Gln Pro Glu Thr Pro Ser Ala Tyr Lys

130 135 140

Glu Gly Ile Ile Ser Ser Thr Ser Asp Thr Asn Asn Pro His Val Leu

145 150 155 160

Gly Tyr Asp Pro Glu Thr Arg Lys Ser Ser Lys Lys Leu Tyr Glu Leu

165 170 175

Met His Asp Phe Asp Thr Ala Tyr Tyr Asp Asp Glu Asn Met Ile Leu

180 185 190

Gly Thr Asn Leu Phe Ser Glu Asn Val Tyr Ser Arg Gln Pro Tyr Val

195 200 205

Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Thr Gly Phe Gly Tyr

210 215 220

Ala Leu Asp Thr Leu Asn Leu Phe Thr Asp Ala Glu Gly Ser Leu Asn

225 230 235 240

Asn Gly Trp Pro Leu Arg Asn Arg Arg Tyr Ala Gly Ala Phe Val Ser

245 250 255

Asp Phe Tyr Ser Leu Gln Glu Lys Leu Asn Ser Thr Asn Phe Pro Glu

260 265 270

Leu Asp Asn His Gly Tyr Ser Thr Val Ile Ser Ser Ile Pro Gln Trp

275 280 285

Thr Asn Leu Gln Phe Ser Leu Asp Asn Gly Ser Ile Trp Phe Asn Pro

290 295 300

Gln Glu Ile Asp Lys Thr Asp Val Ser Asn Tyr Gln Gln Asn Leu Ser

305 310 315 320

Met Lys Asp Gly Ile Val Thr Thr Asp Met Asp Trp Ile Asp Gly Met

325 330 335

Ile His Val Lys Ser Glu Ile Trp Ala His Arg Lys Ile Tyr Pro Leu

340 345 350

Gly Met Val Ser Leu Glu Ile Ser Leu Asn Val Asp Ser Ile Pro Asp

355 360 365

Gly Phe Glu Ser Val Asp Leu Asp Val Trp Asp Val Leu Asp Phe Asn

370 375 380

Thr Ser His Arg Thr Val Leu Asn Asp Ile Gly Tyr Asp Asp Asp Gly

385 390 395 400

Ile Tyr Met Val Ile Glu Pro Asp Asn Val Pro Tyr Ser Asn Ala Ala

405 410 415

Leu Tyr Ser Ala Cys Thr Leu Gln Leu Glu Asn Ser Thr Glu Glu His

420 425 430

Pro Phe Gln Phe Asp Glu Asp Lys Asp Pro Leu Met Asn Thr Asn Glu

435 440 445

Arg Ile Ser Lys Val Ser His Val Thr Leu Ser Lys Ala Asn Pro Lys

450 455 460

Leu Thr Val His Lys Tyr Val Gly Ile Met Ser Thr Glu Tyr Asn Asn

465 470 475 480

Asp Glu Lys Asn Gly Gly Ala Ser Asn Leu Asp Leu Ala Thr Lys Val

485 490 495

Ala Met Ser Ser Lys Gly Ser Tyr Asp Leu Leu Leu Ser Thr His Lys

500 505 510

Lys Ala Trp Tyr Ala Leu Tyr Asn Asp Ala Phe Ile Glu Ile Pro Ser

515 520 525

Asp Asn Leu Leu Glu Met Thr Ala Arg Ser Ser Leu Tyr Gln Leu Leu

530 535 540

Ala Asn Thr Arg Thr Tyr Asn Val Ser Glu Glu Arg Gly Leu Pro Leu

545 550 555 560

Ala Val Gly Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp

565 570 575

Asp Ser Asp Ile Trp Val Gln Pro Ala Leu Leu Pro Phe Phe Pro Gln

580 585 590

Ile Ala Lys Asn Ile Asn Asn Tyr Arg Asn Ala Thr His Ala Gln Ala

595 600 605

Arg Leu Asn Ala Lys Gln Tyr Gly Tyr Pro Gly Ala Val Tyr Pro Trp

610 615 620

Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser Ala Gly Pro Cys Val Asp

625 630 635 640

Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Met Ala Thr Phe Ser Ile

645 650 655

Tyr Met Asn Gly Gly Lys Asp Val Asp Asp Asn Tyr Leu Arg Tyr Thr

660 665 670

Thr Trp Pro Met Val Arg Asp Ala Ala Glu Phe Phe Thr Val Tyr Val

675 680 685

Lys Tyr Asn Ala Thr Leu Gly Lys Tyr Glu Thr Tyr Asn Leu Thr Asp

690 695 700

Pro Asp Glu Phe Ala Asp His Ile Asn Asn Gly Ala Phe Thr Asn Ala

705 710 715 720

Gly Ile Lys Thr Leu Leu Lys Trp Ala Thr Asp Ile Gly Asn His Leu

725 730 735

Gly Glu Tyr Val Asp Pro Lys Trp Met Glu Ile Ser His Asn Ile Tyr

740 745 750

Ile Pro Arg Ser Lys Ser Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn

755 760 765

Ser Ser Val Glu Ile Lys Gln Ala Asp Val Thr Leu Met Val Tyr Pro

770 775 780

Leu Gly Tyr Ile Asn Asp Glu Ser Ile Leu Asn Asn Ala Ile Lys Asp

785 790 795 800

Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr

805 810 815

Tyr Pro Val Phe Val Ala Ala Ala Ser Thr Leu Leu Asn His Gly Ser

820 825 830

Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val Leu Pro Tyr Leu Arg Ala

835 840 845

Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Ser Thr Asn

850 855 860

Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Phe

865 870 875 880

Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile Arg Tyr Thr Tyr Glu

885 890 895

Val Asp Glu Ala Thr Lys Lys Ile Asn Arg Val Leu Arg Phe Asn Pro

900 905 910

Val Glu Leu Pro Leu Leu Pro Gly Gly Ile Ala Ile Arg Asn Phe Lys

915 920 925

Tyr Met Asp Gln Val Leu Asp Ile Ile Leu Asn Asp His Asn Gly Thr

930 935 940

Ile Val His Lys Ser Gly Gly Lys Pro Ile Asn Ile Lys Val Pro Asn

945 950 955 960

Arg Lys Leu Ile His Asp Arg Asp Ile Asp Leu Phe Thr Arg Asp Glu

965 970 975

Lys Arg Glu Ser Leu Glu Arg Arg Asp Gln Tyr Glu Lys Glu Asp Pro

980 985 990

Asn Asp Thr Ser Asn Asp Ala Lys Asp Ser Thr Lys Tyr Gly Thr Ser

995 1000 1005

Tyr Val Leu His Pro Gly Glu Glu Leu Thr Ile Pro Leu Phe Lys

1010 1015 1020

Pro Glu Leu Asn Ile Glu Gly Asn Ile Ala Glu Ser Lys Gln Ile

1025 1030 1035

Thr Asn Leu Thr Ala Gly Val Pro Gly Asp Val Gly Phe Ser Ala

1040 1045 1050

Leu Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Ala Asp Lys Lys

1055 1060 1065

Asp Asn Ala Arg Leu Leu Ile Asp Leu Gly Glu Asp Asn Ile Lys

1070 1075 1080

Glu Ile Arg Lys Gly Met Ile Leu Trp Gly Gln Arg Pro Ala Lys

1085 1090 1095

Asn Ile Ser Ile Ala Ile Leu Pro His Thr Asp Thr Val Glu Asn

1100 1105 1110

Ile Phe Thr Asn Val Ser Lys Ile Leu Glu Asn Ser Lys Asn Ala

1115 1120 1125

Glu Glu Tyr Arg Glu Gly Val Ile Asp Leu Leu Asn Glu Asn Asn

1130 1135 1140

Ile Ser Val Gln Ser Thr Ser Ser Thr Gly Glu Val Glu Ala Asn

1145 1150 1155

Thr Asp Leu Gln Ser Ile Ile Ser Trp Lys Thr Asp Asp Phe Asp

1160 1165 1170

Glu Val Leu Glu Arg Phe Pro Arg Leu Gly Ile Leu Gly Glu Asn

1175 1180 1185

Phe Val Thr Ile Leu Gln Asp Tyr Lys Ile Glu Pro Ser Glu Pro

1190 1195 1200

Tyr Tyr Glu Glu Val Ile Lys Glu Ser Leu Ile Glu Ile Leu Pro

1205 1210 1215

Ser Asn Lys Thr Glu Phe Ser Ile Asp Tyr Ser Arg Ile His Ile

1220 1225 1230

Asn Ser Thr Arg Asn Asn Thr Glu Ile Gly Glu Trp Lys Lys Ala

1235 1240 1245

Arg Tyr Val Leu Leu Thr Val His Gly Thr Tyr Asp Asp Asp Ile

1250 1255 1260

Asp Pro Lys Gly Ala Thr Ile Lys Glu Ile Ala Phe Met Glu Gln

1265 1270 1275

<210> 66

<211> 1152

<212> PRT

<213> 耐热拉茜斯酵母(Lachancea thermotolerans)

<400> 66

Met Ser Val Arg His Lys Thr Arg Lys His Thr Ala Thr Ser Leu Ile

1 5 10 15

Ala Arg Ser Leu Pro Gly Leu Arg Ala Ile Phe Val Ile Leu Ala Thr

20 25 30

Cys Val Ala Ala Ala Gly Trp Arg Trp Leu Leu Thr Gly Glu Leu Pro

35 40 45

Arg Lys Pro His Leu Pro Cys Lys Val Phe Arg Thr His Arg His His

50 55 60

Asp Thr Arg Ile Glu Trp Arg Arg Arg Ser Ser Glu Ser Leu Tyr Glu

65 70 75 80

Leu Leu Thr Asp Ser Gln Asp Thr Tyr Tyr Asp Glu Asp Asn Trp Thr

85 90 95

Val Gly Thr Val Phe Phe Ser Glu Asn Thr Tyr Ser Arg Gln Pro Tyr

100 105 110

Val Ala Asn Gly Phe Leu Gly Ser Arg Ile Pro Asn Val Gly Phe Gly

115 120 125

Tyr Ala Pro Asp Thr Tyr Asn Ile Trp Thr Pro Asp Pro Ser Ile Pro

130 135 140

Gly Ala Leu Asn Asn Gly Trp Pro Leu Arg Asn Gln Arg Phe Ala Gly

145 150 155 160

Ala Phe Val Ser Asp Phe Tyr Ser Leu Gln Pro His Leu Asn Ser Thr

165 170 175

Asn Phe Pro Glu Leu Asp Ala Asp Gly Tyr Ser Thr Ile Leu Ser Ala

180 185 190

Ile Pro Gln Trp Thr Asp Met Lys Ile Ser Val Lys Asn Asp Thr Val

195 200 205

Arg Phe Thr Ala Gln Asp Val Leu Pro Gln His Val Ser Asn Tyr His

210 215 220

Gln Asn Leu Ser Leu Leu Thr Gly Thr Val Thr Thr Glu Leu Asp Trp

225 230 235 240

Met Gly Leu Leu His Ile Lys Ser Thr Val Leu Ala His Arg Ile Leu

245 250 255

His Pro Leu Gly Val Leu Lys Leu Glu Leu Ser Leu Ile Pro Asp Ser

260 265 270

Gly Leu Lys Thr Leu Asp Leu Gln Val Ser Asp Ile Phe Asn His Ser

275 280 285

Thr Ser Arg Arg Thr Phe Leu Arg Asp Leu Gly Tyr Asp Ser Arg Gly

290 295 300

Ile Tyr Met Glu Val Glu Pro Asp Asn Val Pro Tyr Ser Ser Ala Ala

305 310 315 320

Leu Tyr Ser Ser Leu Arg Phe Asp Gly Pro Ala Glu Ala Asp Gly Cys

325 330 335

Ile Phe Gln Leu Ser Asp Met Asn Thr Thr Val Ser Arg Lys Asn Phe

340 345 350

Leu Thr Leu Ser Cys Glu Lys Pro Ser Ile Thr Ile Gln Lys Tyr Val

355 360 365

Ala Val Val Ser Thr Glu Tyr Asp Arg Tyr Asn Lys Thr Ala Ser Asn

370 375 380

Met Glu Ala Ala Lys Asp Ile Val His Ser Gln Cys Gly Gln Tyr Asp

385 390 395 400

Leu Ile Thr Gln Ala His His Asn Glu Trp Val Ser Leu Tyr Asp Asn

405 410 415

Ala Ser Ile Glu Ile Pro Ser Asp Ser Leu Leu Glu Leu Ser Ala Lys

420 425 430

Ser Ser Leu Phe His Leu Leu Ala Asn Thr Arg Arg Phe Asn Ile Ser

435 440 445

Ser Ser Arg Gly Leu Pro Leu Pro Ser Ser Gly Leu Ser Ser Asp Ser

450 455 460

Tyr Gly Gly Met Ile Phe Trp Asp Ala Asp Ile Trp Ile Leu Pro Ala

465 470 475 480

Leu Leu Pro Phe Phe Pro Asp Ile Ala Arg His Met Val Asp Tyr Arg

485 490 495

Lys Asn Thr His Glu Gln Ala Arg Leu Asn Ala Gln Gln Tyr Gly Tyr

500 505 510

Pro Gly Ala Leu Tyr Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr

515 520 525

Ser Thr Gly Pro Cys Val Asn Tyr Glu Tyr His Ile Asn Ile Asp Ile

530 535 540

Ala Met Ala Ser Phe Leu Val Tyr Leu Asn Gly Ala Pro Gly Val Asp

545 550 555 560

Asp Glu Tyr Leu Arg Glu Thr Thr Trp Pro Leu Val Arg Asp Ala Ala

565 570 575

Gln Phe Phe Thr Ala Tyr Val Lys Phe Asn Ser Ser Leu Asp Ala Tyr

580 585 590

Glu Thr His Asn Met Thr Asp Pro Asp Glu Tyr Ala Asn Phe Ile Asn

595 600 605

Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp Ala

610 615 620

Thr Asp Val Gly Asn His Leu Gln Glu Glu Ile Asp Pro Arg Trp Val

625 630 635 640

Glu Val Ser Asp Lys Met Phe Ile Pro Ile Ala Glu Ser Asn Ile Thr

645 650 655

Leu Glu Tyr Ser Gly Met Asn Ala Ser Val Glu Ile Lys Gln Ala Asp

660 665 670

Val Met Leu Met Thr Tyr Pro Leu Gly Tyr Ile Thr Asp Glu Thr Ile

675 680 685

Leu Asp Asn Ala Ile Arg Asn Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser

690 695 700

Pro Ser Gly Pro Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala

705 710 715 720

Gly Leu Leu Asn His Gly Cys Ser Ser Gln Ser Tyr Leu Tyr Lys Ser

725 730 735

Val Leu Pro Tyr Leu Arg Ala Pro Phe Ala Gln Phe Ser Glu Gln Ser

740 745 750

Asp Asp Asn Phe Leu Thr Asn Gly Phe Thr Gln Pro Ala Phe Pro Phe

755 760 765

Leu Thr Gly Asn Gly Gly Tyr Leu Gln Ser Leu Leu Phe Gly Leu Thr

770 775 780

Gly Leu Arg Tyr Ser Tyr Glu Val Asp Lys Gln Thr Lys Lys Ile Thr

785 790 795 800

Arg Leu Leu Lys Phe Asn Pro Ile Lys Leu Pro Leu Leu Gln Gly Gly

805 810 815

Ile Ala Ile Asn Asn Phe Lys Tyr Met Gly Gln Val Leu Asp Ile Ile

820 825 830

Ile Gly Asp Glu Lys Ala Thr Ile Val His Lys Ile Gly Asp Glu Pro

835 840 845

Ile Asn Leu Lys Leu Ile Asp Arg Ser Phe Ile His Asp Arg Asp Val

850 855 860

Thr Gly Pro His Met Thr His Val His Glu Gly Glu Glu Val Pro Val

865 870 875 880

Gln Trp Ile Thr Leu Gln Pro Gly Gln Asn Leu Thr Val Asp Leu Phe

885 890 895

Arg Pro Leu Leu Asn Ile Asp Gly Asn Leu Ile Glu Gly Lys Gln Val

900 905 910

Ser Asn Phe Thr Ala Gly Val Pro Gly Asp Val Ile Tyr Ser Val Ile

915 920 925

Asp Gly Asn Asn Phe Thr His Trp Gln Pro Leu Asp Lys Asn Val Cys

930 935 940

Ala Lys Ile Leu Ile Asp Leu Gly Pro Gly Asn Glu Gln Asn Leu Thr

945 950 955 960

Gly Gly Met Ile Leu Trp Gly Arg Arg Pro Ala Arg Asn Ile Ser Val

965 970 975

Ala Met Val Pro Tyr Lys Gly Asp Ile Glu Gln Ala Leu Gln Lys Ala

980 985 990

Ser Ser Val Met Glu Ser Ser Gly Pro Asp Lys Arg Val Glu Ile Leu

995 1000 1005

Gly Tyr Thr Ala Pro Ala Ser Glu Leu Ala Ser Arg Leu Ser Glu

1010 1015 1020

Thr Glu Asp Arg Phe Phe Glu Glu Asp Tyr Arg Leu Leu Leu Ser

1025 1030 1035

Ser Gln Arg Leu Asp Ile Glu Gln Leu Ser Lys Leu Ser Pro Glu

1040 1045 1050

Ser Ser Leu Leu Lys Ser Lys Phe Val Thr Ile Leu His Asn Leu

1055 1060 1065

Gln Ile Ser Pro Ser Glu Pro Tyr Ala Glu Glu Asn Gln Arg Gly

1070 1075 1080

Ser Lys Ile Val Ile Pro Arg Ser Asn Ser Thr His Phe Thr Phe

1085 1090 1095

Asp Tyr Ser Asn Tyr Thr Ser Asp Lys Ala Phe Glu His Pro Val

1100 1105 1110

Ser Lys Glu Ser Gly Ala Ser Trp Pro Pro Pro Arg Phe Val Leu

1115 1120 1125

Val Cys Ile Gln Gly Thr Tyr Asp Asp Asp Asp Asp Pro Lys Gly

1130 1135 1140

Ala Thr Ile Lys Glu Ile Ala Leu Met

1145 1150

<210> 67

<211> 1152

<212> PRT

<213> 魁北克拉茜斯酵母(Lachancea quebecensis)

<400> 67

Met Ser Val Arg His Lys Thr Arg Lys His Thr Ala Thr Asn Leu Ile

1 5 10 15

Val Gly Ser Leu Pro Gly Leu Arg Ala Ile Phe Val Ile Leu Thr Ala

20 25 30

Cys Val Ala Ala Val Gly Trp Arg Trp Leu Leu Thr Gly Lys Leu Pro

35 40 45

Lys Lys Pro His Leu Pro Cys Lys Val Phe Arg Thr His Arg His His

50 55 60

Asp Thr Arg Ile Glu Trp Arg Arg Arg Ser Ser Glu Ser Leu Tyr Glu

65 70 75 80

Leu Leu Thr Asp Ser Gln Asp Thr Tyr Tyr Asp Glu Asp Asn Trp Ile

85 90 95

Val Gly Thr Val Phe Phe Ser Glu Asn Thr Tyr Ser Arg Gln Pro Tyr

100 105 110

Val Ala Asn Gly Phe Leu Gly Ser Arg Ile Pro Asn Ile Gly Phe Gly

115 120 125

Tyr Ala Pro Asp Ala Tyr Asn Ile Trp Ala Ala Asp Pro Ser Ile Pro

130 135 140

Gly Ala Leu Asn Asn Gly Trp Pro Leu Arg Asn Arg Arg Tyr Ala Gly

145 150 155 160

Ala Phe Val Ser Asp Phe Tyr Ser Leu Gln Pro His Leu Asn Ser Thr

165 170 175

Asn Phe Pro Glu Leu Asp Ala Asn Gly Tyr Ser Thr Val Leu Ser Ala

180 185 190

Ile Pro Gln Trp Thr Asp Met Lys Leu Ser Ile Lys Asn Asp Thr Val

195 200 205

Arg Phe Thr Ala Gln Asp Val Leu Pro Gln His Val Thr Asn Tyr His

210 215 220

Gln Asn Leu Ser Leu Leu Thr Gly Thr Val Thr Thr Glu Leu Asp Trp

225 230 235 240

Met Gly Ile Leu His Ile Lys Ser Thr Val Leu Ala His Arg Thr Leu

245 250 255

His Pro Leu Gly Val Leu Asn Met Glu Leu Ser Leu Leu Ser Asn Ser

260 265 270

Gly Leu Lys Thr Leu Asp Leu Gln Val Ser Asp Ile Phe Asn His Ser

275 280 285

Thr Ser Arg Arg Thr Phe Leu Arg Asp Leu Gly Tyr Asp Ser Gln Gly

290 295 300

Ile Tyr Met Glu Val Glu Pro Asp Asn Val Pro Tyr Ser Ser Ala Ala

305 310 315 320

Met Tyr Ser Ser Leu Arg Phe Ile Gly Ser Gly Glu Ser Asp Gly Cys

325 330 335

Ile Phe Gln Leu Ser Asp Met Asn Thr Thr Val Ser Arg Lys Asn Phe

340 345 350

Leu Thr Leu Ser Asn Glu Lys Pro Ser Ile Thr Ile Gln Lys Tyr Val

355 360 365

Ala Val Val Ser Thr Glu Tyr Asp Arg Tyr Asn Lys Thr Ala Ser Asn

370 375 380

Met Glu Leu Ala Lys Asp Ile Val Arg Ser Gln Cys Gly His Tyr Asp

385 390 395 400

Ser Ile Thr Gln Met His His Asp Glu Trp Val Ser Leu Tyr Gly Asn

405 410 415

Ala Ser Ile Glu Ile Pro Ser Asp Ser Leu Leu Glu Leu Ser Ala Arg

420 425 430

Ser Ser Leu Phe His Leu Leu Ala Asn Thr Arg Arg Phe Asn Ile Ser

435 440 445

Ser Ser Arg Gly Leu Pro Leu Pro Ser Ser Gly Leu Ser Ser Asp Ser

450 455 460

Tyr Gly Gly Met Ile Phe Trp Asp Ala Asp Leu Trp Ile Leu Pro Ala

465 470 475 480

Leu Leu Pro Phe Phe Pro Asp Ile Ala Arg His Met Val Asp Tyr Arg

485 490 495

Arg Asn Thr His Glu Gln Ala Arg Leu Asn Ala Gln Gln Tyr Gly Tyr

500 505 510

Pro Gly Ala Leu Tyr Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr

515 520 525

Ser Thr Gly Pro Cys Val Asp Tyr Glu Tyr His Ile Asn Ile Asp Ile

530 535 540

Ala Met Ala Ser Phe Leu Val Tyr Leu Asn Gly Ala Pro Gly Val Asp

545 550 555 560

Asp Glu Tyr Leu Arg Glu Thr Thr Trp Pro Leu Val Arg Asp Ala Ala

565 570 575

Leu Phe Phe Thr Ala Tyr Val Lys Tyr Asn Ser Ser Leu Asp Ala Tyr

580 585 590

Glu Thr His Asn Met Thr Asp Pro Asp Glu Tyr Ala Asn Phe Ile Asn

595 600 605

Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp Ala

610 615 620

Thr Asp Val Gly Asn His Leu Gln Glu Glu Val Asp Pro Arg Trp Val

625 630 635 640

Glu Val Ser Asn Lys Met Phe Ile Pro Ile Ala Glu Ser Asn Ile Thr

645 650 655

Leu Glu Tyr Ser Gly Met Asn Ala Ser Val Glu Ile Lys Gln Ala Asp

660 665 670

Val Val Leu Met Thr Tyr Pro Leu Gly Tyr Ile Thr Asp Glu Thr Ile

675 680 685

Leu Asp Asn Ala Ile Arg Asn Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser

690 695 700

Ala Ser Gly Pro Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala

705 710 715 720

Gly Leu Leu Asn His Gly Cys Ser Ser Gln Ser Tyr Leu Tyr Lys Ser

725 730 735

Val Leu Pro Tyr Leu Arg Ala Pro Phe Ala Gln Phe Ser Glu Gln Ser

740 745 750

Asp Asp Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe

755 760 765

Leu Thr Gly Asn Gly Gly Tyr Leu Gln Ser Leu Leu Phe Gly Leu Thr

770 775 780

Gly Leu Arg Tyr Ser Tyr Glu Val Asp Gln Lys Thr Lys Lys Ile Thr

785 790 795 800

Arg Leu Leu Lys Phe Asn Pro Ile Lys Leu Pro Leu Leu Gln Gly Gly

805 810 815

Ile Val Ile Asn Asn Phe Lys Tyr Met Gly Gln Val Leu Asp Ile Ile

820 825 830

Val Gly Asp Glu Asn Ala Thr Ile Ala His Lys Val Gly Asp Gln Pro

835 840 845

Ile Asn Leu Lys Leu Ile Asp Arg Asn Phe Ile His Asp Arg Asp Val

850 855 860

Ala Ile Ser Asp Ile Lys His Val His Glu Ser Glu Lys Val Pro Val

865 870 875 880

Gln Arg Val Thr Leu Gln Pro Gly Gln Asn Leu Thr Val Asp Leu Phe

885 890 895

His Pro Leu Thr Asn Ile Asp Gly Asn Leu Val Glu Gly Thr Gln Val

900 905 910

Ser Asn Phe Thr Ala Gly Val Pro Gly Asp Val Ile Tyr Ser Val Ile

915 920 925

Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Leu Asn Lys Asn Ala Cys

930 935 940

Ala Lys Ile Leu Ile Asp Leu Gly Pro Gly Asn Glu Gln Asn Leu Thr

945 950 955 960

Gly Gly Met Ile Leu Trp Gly Arg Arg Pro Ala Arg Asn Ile Ser Ile

965 970 975

Ala Met Val Pro Tyr Lys Gly Asp Ile Glu Gln Ala Leu Glu Lys Ala

980 985 990

Ser Lys Val Ile Glu Ser Asn Gly Pro Gly Lys Arg Val Glu Ile Leu

995 1000 1005

Gly Tyr Thr Ala Pro Ala Ser Val Leu Ala Ser Arg Leu Ser Glu

1010 1015 1020

Ala Lys Asp Trp His Phe Glu Glu Asp Tyr Arg Leu Leu Leu Ser

1025 1030 1035

Ser Gln Arg Leu Asp Ile Glu Gln Leu Ser Lys Leu Ser Pro Asp

1040 1045 1050

Ser Ser Leu Leu Asp Ser Lys Phe Val Thr Ile Leu Asn Asn Leu

1055 1060 1065

Gln Ile Ser Pro Ser Glu Pro Tyr Ala Glu Glu Asn Gln Arg Gly

1070 1075 1080

Ser Lys Ile Val Ile Pro Arg Ser Asn Ser Thr His Phe Thr Phe

1085 1090 1095

Asp Tyr Ser Ser Tyr Thr Asn Asn Thr Thr Phe Asp His Leu Thr

1100 1105 1110

Ser Asn Asp Ser Gly Ala Ser Trp Pro Pro Pro Arg Phe Val Leu

1115 1120 1125

Val Cys Ile Gln Gly Thr Tyr Asp Asp Asp Asp Asp Pro Arg Gly

1130 1135 1140

Ala Thr Ile Lys Glu Ile Ala Leu Met

1145 1150

<210> 68

<211> 1202

<212> PRT

<213> 毕赤四盾酵母(Tetrapisispora phaffii)

<400> 68

Met Phe Phe Asp Asn Val Tyr Ser Gln Arg Lys Glu Lys Asp Leu Val

1 5 10 15

Leu Leu Ile Trp Phe Gln Arg Ile Phe Ile Ile Met Leu Leu Ile Ile

20 25 30

Phe Leu Ile Phe Asn Cys Phe Ile Thr Val Gln Ser Ile His Ile Asn

35 40 45

Ser Phe Phe Asn Asn Lys Phe Leu Arg His Phe Ser Thr Arg Asn Asn

50 55 60

Lys Leu Ile Tyr Ala Ser Pro Ile Ala Gln Ser Asn Leu Ser Ile Leu

65 70 75 80

Ser Val Gly Glu Glu Ile Ser Gln Val Ala Thr Ala Arg Lys Thr Ser

85 90 95

Lys Lys Leu Tyr Asp Leu Leu Ile Asn Tyr Glu Asp Ser Tyr Tyr Asp

100 105 110

Asp Glu Asn Met Val Leu Gly Thr Asp Val Ile Thr Lys Asn Thr Tyr

115 120 125

Thr Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro

130 135 140

Asn Ile Gly Phe Gly Tyr Ala Leu Asp Glu Tyr Ser Ile Trp Asn Pro

145 150 155 160

Asp Val Thr Asp Asn Pro Glu Leu Phe Asn Asn Gly Trp Pro Leu Arg

165 170 175

Asn Arg Arg Tyr Ala Gly Ser Phe Val Ser Asp Phe Tyr Ser Leu Gln

180 185 190

Lys Lys Leu Asn Ser Thr Asn Phe Pro Glu Ile Asp Asp Ile Gly Tyr

195 200 205

Ser Asn Val Ile Ser Ser Ile Pro Glu Trp Thr Asn Ile Lys Leu Thr

210 215 220

Val Ile Asp Glu Asn Asp Arg Ser Phe Asn Phe Asn Pro Gln Asp Ile

225 230 235 240

Ser Val Asn Asp Ile Thr Asn Tyr His Gln Asn Leu Ser Met Gln Asn

245 250 255

Gly Met Val Thr Thr Asp Leu Asn Trp Leu Asn Asn Thr Leu His Ile

260 265 270

Asn Ser Thr Met Phe Thr His Arg Lys Leu Tyr Pro Leu Gly Leu Leu

275 280 285

Glu Ile Asp Ile Thr Leu Asn Arg Glu Tyr Leu Pro Thr Asp Phe Ala

290 295 300

Thr Tyr Asn Ile Ser Val Phe Asp Ile Leu Asp Phe Asn Thr Ser His

305 310 315 320

Arg Thr Phe Leu Arg Asp Met Gly Pro Ser Tyr Asp Ser Ser Lys Met

325 330 335

Gln Gly Ile Phe Met Val Val Glu Pro Glu Asn Val Pro Tyr Thr Asn

340 345 350

Cys Val Ile Phe Ser Thr Thr Glu Ile Glu Gln Leu Asp Ile Thr Gly

355 360 365

Ser Val His Arg Asn Ile Thr Leu Asp Asp Tyr Val Ile Asp Gly Met

370 375 380

Asn Ser Ser Ile Ser Gln Val Ala Lys Asp Ile Thr Leu Ser Thr Glu

385 390 395 400

Phe Pro Lys Leu Lys Ile Tyr Lys Tyr Ser Ser Ile Ile Ser Ser Asn

405 410 415

Tyr Asp Gly Cys Lys Asn Asn Thr Tyr Gln Asp Asn Leu Asn Glu Ala

420 425 430

Lys His Leu Ala Leu Asn Ser Arg Gly Asn Phe Gln Asn Leu Ala Asp

435 440 445

Ser Asn Thr Gln Ala Trp Val Asp Leu Tyr Asp Asn Ala Phe Ile Glu

450 455 460

Ile Pro Ser Asp Ser Val Leu Glu Leu Ala Ala Arg Ser Ser Leu Phe

465 470 475 480

His Leu Met Ser Asn Ala Arg Leu His Asn Ile Ser Glu Asp Arg Gly

485 490 495

Leu Pro Leu Pro Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Leu

500 505 510

Val Phe Trp Asp Ala Asp Val Trp Met Gln Pro Ala Ile Leu Pro Phe

515 520 525

Phe Pro Asp Val Ala Lys Asn Ile Asn Asn Tyr Arg Asn Ala Thr His

530 535 540

Lys Gln Ala Gln Leu Asn Ala Lys Gln Tyr Gly Tyr Asn Gly Ala Trp

545 550 555 560

Tyr Pro Trp Thr Ser Gly Gly Phe Ser Asn Cys Thr Ser Thr Gly Pro

565 570 575

Cys Ile Asp Tyr Glu Tyr His Ile Asn Val Asp Ile Ala Leu Ala Thr

580 585 590

Phe Ser Ile Tyr Met Asn Gly Ala Asp Gly Ile Asp Asp Thr Tyr Leu

595 600 605

Lys Tyr Thr Val Trp Pro Ile Val Arg Asp Ala Ala Ile Ala Phe Thr

610 615 620

Glu Tyr Val Gln Tyr Asp Lys Gln Leu Asp Val Tyr Glu Thr Phe Asn

625 630 635 640

Leu Thr Asp Pro Asp Glu Phe Ala Asn His Val Asn Asn Gly Ala Phe

645 650 655

Thr Asn Ala Gly Ile Lys Ile Leu Met Lys Trp Ala Val Asp Ile Gly

660 665 670

Asn His Leu Glu Glu Lys Val Asp Pro Ile Trp Lys Ala Ile Asn Glu

675 680 685

Lys Ile Val Ile Pro Lys Thr Ser Ser Asn Ile Thr Leu Glu Tyr Ser

690 695 700

Gly Met Asn Ser Thr Ala Glu Ile Lys Gln Ala Asp Val Pro Leu Leu

705 710 715 720

Val Tyr Pro Leu Gly Tyr Ile Thr Asp Glu Ser Ile Leu Asn Asn Ala

725 730 735

Ile Arg Asp Leu Tyr Tyr Tyr Ser Glu His Gln Ala His Thr Gly Pro

740 745 750

Ala Met Thr Tyr Pro Val Phe Val Ala Ala Ala Ala Gly Leu Leu Asn

755 760 765

His Gly Ser Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Ile Leu Pro Tyr

770 775 780

Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe

785 790 795 800

Leu Glu Asn Gly Gly Leu His Pro Ala Tyr Pro Phe Leu Thr Ala Asn

805 810 815

Gly Gly Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile Arg Tyr

820 825 830

Ser Tyr Lys Val Asp Ser Asp Thr Asn Lys Ile Ala Arg Leu Leu Lys

835 840 845

Phe Asn Pro Ile Lys Leu Lys Leu Leu Pro Gly Gly Leu Lys Ile Arg

850 855 860

Asn Phe Lys Tyr Met Gly Gln Val Leu Asp Ile Ile Ile Asp Asp Tyr

865 870 875 880

Asn Gly Thr Ile Ile His Lys Ser Gly Asn Lys Thr Ile Thr Ile Gln

885 890 895

Ile Pro Asp Arg Ser Ser Ile Arg Asp Gln Asp Ile Asn Pro Tyr Arg

900 905 910

Gly Lys Asn Lys Thr Lys Glu Asp Asn Arg Ile Leu Ser Lys Arg Lys

915 920 925

Ser Asn Ile Tyr Glu Leu Lys Pro Asn Glu Asn Phe Thr Ile Pro Leu

930 935 940

Phe Val Thr Glu Leu Asn Ala Pro Gly Asn Ile Val Glu Ser Lys Gln

945 950 955 960

Ile Thr Asn Leu Thr Glu Gly Val Ala Gly Asp Val Ala Met Ser Ala

965 970 975

Val Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Val Asn Lys Ala Glu

980 985 990

Thr Ala Arg Leu Leu Ile Asp Leu Gly Pro Gly Asn Glu Gln Thr Ile

995 1000 1005

Thr Gly Gly Met Phe Leu Trp Gly Gln Arg Pro Ala Lys Asn Val

1010 1015 1020

Ser Ile Ser Val Leu Pro His Ser Gln Ala Thr Glu Gln Leu Phe

1025 1030 1035

Gln Asn Ile Thr Tyr Phe Ala Glu His Phe Glu Phe Pro Asn Arg

1040 1045 1050

Glu Arg Thr Ile Leu Gln Leu Ile Gln Glu Val Asp Asn Ile Thr

1055 1060 1065

Glu Pro Glu Val Cys Gln Ile Phe Ser Ser Asp Gly Val Cys Asp

1070 1075 1080

Ala Gly Phe Thr Gln Leu Leu Asn Trp Lys Ser Lys Asp Ile Asp

1085 1090 1095

Glu Leu Ile Gln Gln Phe Pro Asn Thr Pro Gly Ile Arg Glu Asn

1100 1105 1110

Phe Ile Thr Ile Val Asp Asn Leu Ser Val Leu Pro Ser Glu Pro

1115 1120 1125

Tyr Tyr Pro Glu Tyr Tyr Glu Gln Ala Leu Ile Glu Ile Leu Pro

1130 1135 1140

Ser Asn Lys Thr Lys Phe Ser Val Asp Tyr Ser Lys Val Glu Tyr

1145 1150 1155

Tyr Lys Lys Ser Glu Val Phe Glu Gln Ser Gln Trp Pro Lys Thr

1160 1165 1170

Arg Phe Ile Ile Val Ser Ile Gln Asp Thr Tyr Asp Lys Asp Leu

1175 1180 1185

Asp Pro Lys Gly Ser Thr Ile Lys Glu Ile Ile Leu Tyr Ser

1190 1195 1200

<210> 69

<211> 1156

<212> PRT

<213> 发酵拉茜斯酵母(Lachancea fermentati)

<400> 69

Met Val Gly Ser Ile Asn Asn Arg Thr Cys Phe Ser Pro Tyr Ser Arg

1 5 10 15

Ser Val Leu Tyr Ser Glu Asn Pro Ser Met Lys Gly Arg Phe Gln Ala

20 25 30

Ser Arg Arg Phe Thr Tyr Leu Gly Phe Leu Thr Thr Leu Ala Val Leu

35 40 45

Ser Trp Leu Thr Leu Ala Asp Val His Asn Asp Asp Glu Val Thr Glu

50 55 60

Ser Ala Leu Leu Ser Asp Arg Leu Lys Phe Arg Asp Leu Ser Ser Glu

65 70 75 80

Asn Ile Glu Asn Ser Arg Lys Ala Ser Gln Glu Leu Glu Lys Gln Ile

85 90 95

Val Asp Ser Glu Asp Thr Tyr Tyr Asp Glu Gln Arg Arg Ile Leu Gly

100 105 110

Thr Thr His Phe Ser Glu Asn Thr Tyr Ser Arg Gln Pro Tyr Val Ala

115 120 125

Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Val Gly Phe Gly Tyr Ala

130 135 140

Leu Asp Thr Leu Asn Leu Trp Thr Pro Asn Ser Thr Val Ala Gly Ala

145 150 155 160

Leu Asp Asn Gly Trp Pro Leu Arg Asn Gln Arg Phe Ala Gly Ala Phe

165 170 175

Val Ala Asp Phe Tyr Ser Leu Gln Ala His Leu Asn Ser Thr Asn Phe

180 185 190

Pro Glu Leu Asp Lys Lys Gly Tyr Thr Thr Val Ile Thr Ala Ile Pro

195 200 205

Gln Trp Thr Asp Leu Ser Phe Ser Leu Asn Asp Thr Phe Lys Phe Asn

210 215 220

Pro Val Asp Val His Ser Asp Ser Ile Thr Asn Tyr Lys Gln Asn Leu

225 230 235 240

Ser Met Ser Glu Gly Ile Val Thr Thr Glu Met Asp Trp His Asp Leu

245 250 255

Leu His Ile Glu Thr Thr Ile Phe Ala His Arg Thr Ile His Pro Leu

260 265 270

Gly Val Val Ser Met Asp Ile Thr Leu Asn Thr Ser Ala Asn Leu Asp

275 280 285

Gly Ala Asp Tyr Leu Asp Leu Gln Ile Thr Asp Lys Leu Asn Tyr Ser

290 295 300

Thr Ser Arg Arg Ala Phe Leu Arg Asn Leu Gly Tyr Glu Thr Gly Ser

305 310 315 320

Ile Tyr Met Thr Val Glu Pro Asp Asn Val Pro Tyr Ser Ser Ala Val

325 330 335

Leu Tyr Ser Ser Leu Glu Phe Pro Ser Gln Ser Leu Leu Asn Gly Thr

340 345 350

Leu Tyr Arg Ile Asn Asp Asn Asn Ser Thr Val Ser Gln Asn Gln Tyr

355 360 365

Val Arg Ile Thr Lys Asp Ser Pro Gln Ile Asn Ile Lys Lys Tyr Val

370 375 380

Gly Ile Val Ser Ser Glu Phe Pro Arg Asp Asp Asn Ser Thr Asn Phe

385 390 395 400

Glu Leu Ser Lys Gln Ile Val Glu Glu Asn Ile Gly His Tyr Asp Ser

405 410 415

Leu Leu Ala Ser His Ile Gln Gly Trp Ser Asp Leu Tyr Asn Asn Thr

420 425 430

Glu Val Glu Ile Pro Ser Asp Ser Leu Leu Glu Leu Ala Ala Arg Ser

435 440 445

Ser Ile Phe His Leu Leu Ala Asn Thr Arg Ser Glu Asp Val Ser Pro

450 455 460

Glu Arg Gly Leu Pro Leu Pro Val Ala Gly Leu Ser Ser Asp Ser Tyr

465 470 475 480

Gly Gly Met Val Phe Trp Asp Ala Asp Ile Trp Met Met Pro Ser Leu

485 490 495

Leu Pro Phe Phe Pro Glu Ile Ala Lys Gln Ile Val Asn Tyr Arg Asn

500 505 510

Val Thr His Ser Gln Ala Val Leu Asn Ala Gln Glu Asn Asp Tyr Leu

515 520 525

Gly Ala Val Tyr Pro Trp Thr Ser Gly Arg Tyr Ala Asn Cys Thr Ser

530 535 540

Thr Gly Pro Cys Ile Asp Tyr Glu Tyr His Ile Asn Val Asp Ile Ala

545 550 555 560

Met Ser Ser Phe Leu Leu Tyr Leu Ser Gly Gly Ala Glu Ala Asp Glu

565 570 575

Asp Tyr Leu Arg Tyr Thr Thr Trp Pro Ile Leu Arg Asp Ala Ala Arg

580 585 590

Phe Phe Thr Ser Tyr Val Lys Phe Asn Ser Thr Leu Gly Met Tyr Glu

595 600 605

Thr Lys Asn Met Thr Asp Pro Asp Glu Phe Ala Asn Phe Val Asp Asn

610 615 620

Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu Leu Lys Trp Ala Thr

625 630 635 640

Asp Val Gly Thr Tyr Leu Lys Tyr Asp Val Asp Ser Lys Trp Ala Glu

645 650 655

Val Ser Glu His Met Phe Ile Pro Ile Ser Gln Ala Asn Ile Thr Leu

660 665 670

Glu Tyr Thr Gly Met Asn Ala Ser Val Glu Ile Lys Gln Ala Asp Val

675 680 685

Val Leu Met Thr Tyr Pro Leu Gly Phe Ile Thr Asp Ala Thr Ile Leu

690 695 700

Asn Asn Ala Ile Lys Asp Leu Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala

705 710 715 720

Ser Gly Pro Ala Met Thr Tyr Pro Ile Phe Val Ala Ala Ala Ser Ser

725 730 735

Leu Leu Asn His Gly Cys Ser Ser Gln Ser Tyr Leu Tyr Lys Ser Val

740 745 750

Leu Pro Tyr Leu Arg Ser Pro Phe Ala Gln Phe Ser Glu Gln Ser Asp

755 760 765

Asp Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro Ala Phe Pro Phe Leu

770 775 780

Thr Ala Asn Gly Gly Phe Leu Gln Ala Ile Tyr Phe Gly Leu Thr Gly

785 790 795 800

Leu Arg Tyr Ser Tyr Lys Leu Asp Asn Ser Thr Gly Glu Leu Val Arg

805 810 815

Phe Leu Lys Phe Asp Pro Val Gln Met Pro Leu Leu Pro Gly Gly Ile

820 825 830

Thr Leu Arg Gly Phe Arg Tyr Met Asp Gln Ile Leu Asp Ile Val Ile

835 840 845

Asp Asp Ala Ala Ala Thr Val Tyr His Lys Ser Gly Asn Ser Ser Val

850 855 860

Ile Ile Glu Ile Ser Asp Arg Ser Ile Ile Tyr Asp Gln Ser Asn Ile

865 870 875 880

Phe Phe Glu Asn Gly Ser Thr Leu Gln Phe Lys Lys Arg Asp Leu Pro

885 890 895

Gly Thr Ser Tyr Thr Leu Glu Pro Gly Ser Gln Leu Val Phe Pro Leu

900 905 910

Phe Lys Pro Asn Leu Asn Ile Asp Gly Asn Ile Ala Glu Gly Lys Gln

915 920 925

Ile Thr Asn Phe Thr Ala Gly Val Ser Gly Asp Val Val Tyr Ser Val

930 935 940

Ile Asp Gly Asn Asn Tyr Thr His Trp Gln Pro Leu Asp Lys Tyr Glu

945 950 955 960

Asn Ala Lys Leu Leu Ile Asp Leu Gly Pro Gly Asn Asp Glu Ala Val

965 970 975

Thr His Gly Met Ile Leu Trp Gly Gln Arg Pro Ala Arg Asn Met Ser

980 985 990

Ile Tyr Ala Leu Leu Leu Ser Glu Gln Thr Glu Leu Leu Leu Gln Asn

995 1000 1005

Thr Ser Ser Thr Asp Gln Tyr Glu Ile Gln Asp Ile Met Ser Pro

1010 1015 1020

Ser Leu Asn Asn Glu Phe Lys Asn Asp Ile Asp Glu Asp Phe Glu

1025 1030 1035

Lys Phe Leu Thr Trp Lys Met Leu Asp Ile Asn Asp Phe Ala Gln

1040 1045 1050

Ile Ala Ser Asn Leu Thr Ile Leu Glu Gly Arg Phe Val Pro Leu

1055 1060 1065

Leu Glu Asn Leu Arg Ile Thr Pro Ser Glu Pro Tyr Tyr Glu Gln

1070 1075 1080

His Glu Lys Asn Ser Glu Ile Asp Ile Leu Pro Ser Asn Arg Thr

1085 1090 1095

Lys Phe Thr Ile Asn Tyr Gln Asn Val Ser Ala Tyr Gly Gly Ala

1100 1105 1110

Asn Ser Ser Asn Asp Asn Ser Ser Ser Ala Phe Ile Pro Arg Tyr

1115 1120 1125

Tyr Leu Ile Ser Val Glu Gly Thr Tyr Asp Asp Asp Asp Asp Asp

1130 1135 1140

Asn Gly Ala Thr Ile Asn Glu Ile Ala Phe Ile Ala Asn

1145 1150 1155

<210> 70

<211> 1134

<212> PRT

<213> 假山毛榉拉茜斯酵母(Lachancea nothofagi)

<400> 70

Met Glu Lys His Lys Ile Lys Gln Ser Arg Lys Gly Cys Met Met Gly

1 5 10 15

Ile Phe Leu Thr Leu Ala Val Leu Cys Ser Trp Val Leu Val Arg Glu

20 25 30

Leu Pro Lys Val Ala Arg Ser Pro Cys Lys Val Phe Arg Ser Lys Lys

35 40 45

Leu Phe His Asp Ala Arg Ile Glu Arg Arg Arg Arg Thr Ser Glu Thr

50 55 60

Leu Tyr Glu Leu Leu Ser Asp Ser Arg Asp Thr Tyr Tyr Asp Glu Glu

65 70 75 80

Ala Trp Val Val Gly Thr Ser Phe Phe Ser Glu Asn Thr Tyr Ser Arg

85 90 95

Gln Pro Tyr Val Ala Asn Gly Tyr Leu Gly Ser Arg Ile Pro Asn Val

100 105 110

Gly Phe Gly Tyr Ala Leu Asp Thr Tyr Asn Ile Trp Thr Pro Asp Ala

115 120 125

Ser Ile Pro Gly Ser Leu Asp Asn Gly Trp Pro Leu Arg Asn Arg Arg

130 135 140

Phe Ala Gly Ala Phe Val Ser Asp Phe Tyr Gly Leu Glu Pro Lys Phe

145 150 155 160

Asn Ser Thr Asn Phe Pro Glu Leu Asp Glu Lys Gly Tyr Asn Ser Val

165 170 175

Leu Cys Ala Ile Pro Gln Trp Thr Asp Ile Lys Phe Ser Ile Lys Asn

180 185 190

Asp Thr Phe Gln Phe Asn Ala Gln Ser Val Asn Pro Ser Gln Ile Ser

195 200 205

Asn Tyr Gln Gln Asn Leu Ser Leu Leu Thr Gly Thr Val Thr Thr Glu

210 215 220

Met Asp Trp Leu Asp Val Leu His Ile Lys Ser Ser Val Phe Ala His

225 230 235 240

Arg Ile Arg His Pro Leu Gly Thr Val Gln Leu Glu Ile Ser Leu Ile

245 250 255

Pro Asn Asn Pro Glu Gln Leu Glu Val Ile Asp Leu Gln Leu Ser Asp

260 265 270

Val Leu Asn His Ser Thr Ser Arg Arg Thr Phe Leu Arg Asp Ile Gly

275 280 285

Tyr Asp Ser Gly Gly Ile Tyr Met Glu Val Glu Pro Asn Asn Val Pro

290 295 300

Tyr Ser Ser Ala Ala Leu Tyr Ser Ser Leu Lys Ile Ser Ser Ala Glu

305 310 315 320

Thr Asn Ile Asp Gly Val Val Phe Gln Leu Asn Asp Gly Gly Thr Ser

325 330 335

Val Thr Cys Lys His Leu Leu Gln Leu Thr Ser Asp Asn Ser Thr Val

340 345 350

Thr Ile Asn Lys Phe Val Gly Ile Val Ser Ser Glu Tyr Asp Arg Tyr

355 360 365

Asn Lys Ala Met Ser Asn Leu Asp Ala Ala Lys Asp Val Val Arg Tyr

370 375 380

Thr Gln Thr Glu Ser Tyr Glu Ser Leu Ala Glu Gly His Gln Gln Glu

385 390 395 400

Trp Ile Lys Leu Tyr Asp Asn Ala Ser Ile Glu Ile Pro Ser Asp Ser

405 410 415

Leu Leu Glu Leu Ala Ala Arg Ser Ser Leu Phe His Leu Leu Ala Asn

420 425 430

Thr Arg Arg Phe Asn Val Ser Gln Glu Arg Gly Leu Pro Val Pro Ala

435 440 445

Ser Gly Leu Ser Ser Asp Ser Tyr Ala Gly Leu Val Phe Trp Asp Ala

450 455 460

Asp Leu Trp Ile Met Pro Ser Leu Leu Pro Phe Phe Pro Asp Ile Ser

465 470 475 480

Lys His Met Val Thr Tyr Arg Gln Ser Thr His Asn Gln Ala Lys Ile

485 490 495

Asn Ala Gln Glu Asn Gly Tyr Pro Gly Ala Leu Tyr Pro Trp Thr Ser

500 505 510

Gly Arg Phe Ala Asn Cys Thr Ser Thr Gly Pro Cys Val Asp Tyr Glu

515 520 525

Tyr His Ile Asn Ile Asp Val Ala Met Ala Ser Phe Leu Val Tyr Leu

530 535 540

Asn Gly Ala Asp Gly Ile Asp Asp Asn Tyr Leu Arg Gly Thr Thr Trp

545 550 555 560

Pro Leu Val Arg Asp Ala Ala Leu Phe Phe Ser Ser Tyr Val Asp Tyr

565 570 575

Asn Pro Ala Leu Asp Ala Tyr Glu Thr His Asn Met Thr Asp Pro Asp

580 585 590

Glu Tyr Ala Asn Phe Ile Ser Asn Gly Ala Phe Thr Asn Ala Gly Ile

595 600 605

Arg Thr Leu Leu Lys Trp Ala Thr Asp Ile Gly His His Leu Asn Glu

610 615 620

Ser Val Asp Pro Lys Trp Ala Asp Val Ser Lys Lys Ile Val Ile Pro

625 630 635 640

Ile Ser Asp Thr Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ala Ser

645 650 655

Val Glu Ile Lys Gln Ala Asp Val Met Leu Met Thr Tyr Pro Leu Gly

660 665 670

Tyr Ile Thr Asp Glu Thr Ile Leu Asp Asn Ala Ile Arg Asn Leu Tyr

675 680 685

Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro

690 695 700

Val Phe Val Ala Ala Ala Ala Gly Leu Leu Asn His Gly Cys Ser Ser

705 710 715 720

Gln Ser Tyr Leu Tyr Lys Ser Val Leu Pro Tyr Leu Arg Ala Pro Phe

725 730 735

Val Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu

740 745 750

Thr Gln Pro Ala Phe Pro Phe Leu Thr Gly Asn Gly Gly Tyr Leu Gln

755 760 765

Ser Leu Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr Glu Ser Asn

770 775 780

Lys Thr Thr Gly Gln Ile Thr Arg Leu Leu Lys Phe Asn Pro Ile Lys

785 790 795 800

Leu Pro Leu Leu Ala Gly Gly Ile Thr Ile Arg Asn Phe Lys Tyr Met

805 810 815

Gly Gln Val Leu Asp Val Glu Ile Gly Asp Glu Asn Ala Thr Leu Val

820 825 830

His Lys Ala Gly Asp Gln Pro Ile Arg Leu Lys Leu Leu Asn Arg Asn

835 840 845

Val Ile His Asp Arg Glu Leu Gly Arg Phe Asp Ser Ser His Val His

850 855 860

Glu Leu Lys Gln Leu Pro Val Arg His Ala Ile Leu Glu Pro Gly Arg

865 870 875 880

Asn Leu Thr Val Lys Leu Phe Arg Pro Leu Leu Asn Ile Asp Asn Asn

885 890 895

Leu Ala Glu Gly Lys Gln Val Thr Asn Phe Thr Thr Gly Val Pro Gly

900 905 910

Asp Val Ile Tyr Ser Val Thr Asp Gly Asn Asn Tyr Thr His Trp Gln

915 920 925

Pro Leu His Lys Cys Asp Cys Ala Lys Ile Leu Ile Asp Leu Gly Ala

930 935 940

Asp Asn Glu Gln Thr Leu Thr Gly Gly Met Ile Met Trp Gly Lys Arg

945 950 955 960

Pro Ala Gln Asn Ile Ser Val Ser Met Phe Pro Phe His Gly Ser Ile

965 970 975

Glu Glu Ala Leu Phe Asn Ala Ser Ala Ile Ile Glu Met Ser Gly Pro

980 985 990

Glu Thr Glu Thr Glu Ile Met Gly Phe Lys Gly Ala Ala Ser Asp Val

995 1000 1005

Lys Leu Lys Leu Lys Glu Thr Lys Glu Asn Cys Phe Glu Glu Asp

1010 1015 1020

Tyr Gln Tyr Leu Met Gln Ser Gln Arg Leu Asp Ile Gly Gln Leu

1025 1030 1035

Ser Lys Ile Ser Pro Glu Ser Ser Leu Leu Lys Ser Lys Phe Val

1040 1045 1050

Thr Ile Leu Arg Asp Gln Lys Val Ser Pro Ser Val Pro Tyr Ile

1055 1060 1065

Glu Asn Asp Glu Asn Lys Asp Arg Ile Met Ile Pro Gln Ser Asn

1070 1075 1080

Thr Thr Gln Phe Thr Phe Asp Tyr Ser Tyr Tyr Ser Asn His Ser

1085 1090 1095

Cys Ala Ala Asp Trp Pro Pro Pro Arg Phe Val Leu Val His Val

1100 1105 1110

Gln Gly Thr Tyr Asp Asn Asp Asp Asp Pro Arg Gly Ala Thr Ile

1115 1120 1125

Lys Glu Ile Ala Leu Met

1130

<210> 71

<211> 1066

<212> PRT

<213> 蟑螂四盾酵母(Tetrapisispora blattae)

<400> 71

Met Ile Phe Asn Ile Leu Phe Ser Ile Phe Phe Asn Ile Phe Ile Ile

1 5 10 15

Leu Gln Tyr Leu Gln Val Val Ser Gly Leu Lys Ser Gln Asn Lys Thr

20 25 30

Asn Asn Leu Tyr Gln Ile Leu Ile Asp Asn Asn Asp Asn Ser Tyr Tyr

35 40 45

Asp Ser Thr Asn Gln Ile Leu Gly Thr Lys Gln Phe Thr Lys Gln His

50 55 60

Ala Tyr Ser Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg

65 70 75 80

Ile Pro Asn Val Gly Phe Gly Tyr Ala Leu Asp Thr Ile Asn Leu Trp

85 90 95

Ala Ala Asn Ser Ser Val Glu Gly Ala Leu Asn Asn Gly Trp Pro Leu

100 105 110

Arg Asn His Arg Tyr Ala Gly Ser Phe Val Ser Asp Phe Phe Ser Gln

115 120 125

Gln Glu Lys Leu Thr Ser Thr Asn Phe Pro Glu Leu Asp Glu Val Gly

130 135 140

Tyr Ser Thr Val Ile Ala Ser Ile Pro Glu Trp Thr Asn Ile Lys Ile

145 150 155 160

Ile Leu Ser Val Asn Gly Thr Asp His Leu Ile Ser Pro Glu Ala Leu

165 170 175

Ile Asp Glu Asn Asn Met Ser Asn Tyr Phe Gln Asn Met Ser Met Ser

180 185 190

Asn Gly Val Val Thr Thr Ser Phe Asn Tyr Leu Asn Asp Leu Val Asn

195 200 205

Ile Lys Thr Glu Val Phe Ala His Arg Glu Ile Ser Tyr Leu Gly Ile

210 215 220

Leu Ser Leu Asn Val Thr Leu Leu Asn Pro Asn Glu Tyr Met Met Asp

225 230 235 240

Gln Ile Asp Ile Gln Val Val Asp Ile Leu Asp Phe Asn Ser Ser His

245 250 255

Arg Thr Val Leu Asn Glu Tyr Gly Tyr Asp Leu Lys Asn Gly Ala Ile

260 265 270

Phe Met Ser Val Ser Pro Glu Asn Thr Asp Asp Ser Val Ala Ser Leu

275 280 285

Tyr Ser Thr Leu Gln Ile Glu Asn Asp Ile Glu Tyr Asn Thr Ala Tyr

290 295 300

Ser Lys Ile Asn Glu Lys Ile Glu His Ser Leu Ser Cys Lys Ile Asp

305 310 315 320

Leu Asn Asn Pro Thr Ile Ile Phe Asn Lys Phe Val Gly Ile Ile Ser

325 330 335

Thr Glu Tyr Gly Asn Asp Ala Asp Phe Asn Ser Thr Phe Lys Met Ala

340 345 350

Lys Ser Ser Ile Leu Asp Leu Ser Leu Asn Tyr Thr Gln Leu Tyr Asp

355 360 365

Ser Asn Tyr Val Ala Trp Lys Lys Leu Leu Glu Asn Thr Asp Leu Ser

370 375 380

Ile Pro Gly Asp Ser Leu Leu Asp Met Thr Ala Lys Ser Ser Ile Phe

385 390 395 400

His Leu Leu Ser Asn Thr Arg Ser Lys Asn Val Ala Asp Asn Arg Gly

405 410 415

Leu Pro Val Ser Val Ser Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met

420 425 430

Val Phe Trp Asp Ser Asp Leu Trp Ile Gln Pro Ser Leu Leu Pro Phe

435 440 445

Phe Pro Lys Ile Ser Asn Lys Met Ile Asn Tyr Arg Asn Ala Thr His

450 455 460

Glu Gln Ala Lys Ile Asn Ala Asn Ser Tyr Gly Tyr Asp Gly Ala Leu

465 470 475 480

Tyr Pro Trp Thr Ser Gly Lys Phe Ala Asn Cys Thr Ser Thr Gly Pro

485 490 495

Cys Phe Asp Tyr Glu Tyr His Ile Asn Ile Asp Ile Ala Leu Ala Ala

500 505 510

Ile Ser Leu Tyr Met Ser Gly Gly Gly Asp Glu Asp Tyr Leu Arg Tyr

515 520 525

Thr Ala Trp Pro Ile Val Asn Asp Ala Ala Lys Phe Phe Ser Glu Tyr

530 535 540

Val Gln Tyr Asn Glu Thr Ser Gly Met Tyr Val Thr Gln Asn Met Thr

545 550 555 560

Asp Pro Asp Glu Phe Ala Asp Phe Ile Asp Asn Gly Ala Phe Thr Asn

565 570 575

Ala Gly Ile Arg Ser Leu Leu Lys Trp Ala Thr Asp Ile Gly His His

580 585 590

Leu Asn Glu Thr Ile Asp Glu Asn Trp Met Glu Ile Ser Lys Asn Leu

595 600 605

Tyr Ile Pro Lys Ala Asp Thr Asn Ile Thr Leu Glu Tyr Thr Gly Met

610 615 620

Asn Ser Thr Thr Lys Val Lys Gln Ala Asp Val Ala Leu Met Val Tyr

625 630 635 640

Pro Leu Gly Phe Ile Thr Asp Glu Ser Asn Leu Asn Asp Ala Val Lys

645 650 655

Asp Leu Phe Tyr Tyr Ser Glu His Gln Ser Ala Ala Gly Pro Ala Met

660 665 670

Thr Phe Pro Val Phe Val Ala Ala Ala Ser Ser Leu Leu Asn His Gly

675 680 685

Thr Ser Ser Glu Ser Tyr Leu Tyr Lys Ser Val Ile Pro Phe Leu Arg

690 695 700

Gly Pro Phe Ala Gln Phe Ser Glu Gln Ala Asp Asp Asp Phe Met Lys

705 710 715 720

Asn Gly Glu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala His Gly Gly

725 730 735

Phe Leu Gln Ser Ile Leu Phe Gly Leu Thr Gly Ile Arg Tyr Ser Tyr

740 745 750

Glu Met Asn Thr Gln Thr Asn Lys Ile Glu Arg Phe Leu Arg Phe Asp

755 760 765

Pro Ile Lys Leu Glu Leu Leu Asn Gly Gly Leu His Ile Gln Gly Phe

770 775 780

His Phe Leu Gly Gln Thr Leu Asp Ile Ser Thr Asp Asp Asp Phe Gly

785 790 795 800

Tyr Ile Lys His Lys Asn Gly Thr Gln Asn Ile Arg Ile Lys Val Pro

805 810 815

Thr Arg Asn Tyr Phe Ser Asp Glu Val Asp Ile Phe Thr Arg Asn Asn

820 825 830

Lys Thr Ser Asn Phe Glu Lys Arg Thr Ile Gly Asn Glu Phe Phe Gly

835 840 845

Arg Glu Glu Glu Lys Ser Lys Ser Tyr Ser Asn Gly Thr Phe Ile Thr

850 855 860

Leu Glu Pro Gly Glu Glu Val Lys Ile Pro Leu Phe Lys Pro Leu Lys

865 870 875 880

Asn Ile Pro Ser Asn Leu Ala Glu Gly Lys Gln Val Ala Asn Leu Thr

885 890 895

Glu Gly Val Pro Gly Asp Val Pro Phe Ser Leu Val Asp Gly Asn Asn

900 905 910

Tyr Thr His Trp Gln Pro Ala Asn Lys Ser Ser Ile Ser Arg Val Ile

915 920 925

Ile Asp Leu Gly Ala Tyr Asn Ser Ser Asn Ile Ser Gly Gly Gln Ile

930 935 940

Leu Trp Gly Ser Arg Pro Ala Lys Tyr Phe Ser Ile Ser Val Ile Pro

945 950 955 960

Ser Val Phe Asn Phe Thr Thr Lys Val Gln Met Asn Lys Ile Ile Glu

965 970 975

Glu Glu Ser Ile Ser Val Ile Asp Asn Gln Val Val Leu Pro Ser Lys

980 985 990

Pro Phe Tyr Ala Glu Ile Val Glu Asp Ser Ile Ile Asp Leu Leu Pro

995 1000 1005

Ser Asn Val Thr Asn Phe Glu Phe Asn Phe Lys Asn Ile Asn Glu

1010 1015 1020

Lys Tyr Pro Asn His Ser Asn Ile Ser Asn Leu Ile Arg Tyr Val

1025 1030 1035

Leu Phe Ser Phe Glu Gly Thr Tyr Asp Asn Asp Thr Glu Val Thr

1040 1045 1050

Gly Ala Thr Val Lys Glu Ile Ser Ile Ile Ser Ser Asp

1055 1060 1065

<210> 72

<211> 697

<212> PRT

<213> 小麦顶囊壳(Gaeumannomyces tritici)

<400> 72

Met Pro Ala Met Ala Pro Leu Trp Lys Leu Ala Thr Ala Ala Ala Ala

1 5 10 15

Ser Ser Leu Ala Val Asn Ala Leu Tyr Ile Asn Gly Ser Glu Val Thr

20 25 30

Pro Cys Asp Ser Pro Ile Tyr Cys His Gly Glu Leu Leu Lys Gln Val

35 40 45

Glu Leu Ala His Pro Phe Ala Asp Ser Lys Thr Phe Val Asp Met Pro

50 55 60

Thr Ile Lys Pro Val Asn Glu Val Leu Ala Ala Phe Asp Lys Leu Gln

65 70 75 80

Lys Pro Leu Ser Asn Asn Thr Glu Leu Gln Asn Phe Leu Arg Glu Asn

85 90 95

Phe Ala Lys Ala Gly Gly Glu Leu Glu Glu Val Pro Asn Ser Glu Leu

100 105 110

Ala Thr Asp Pro Val Phe Leu Asn Lys Leu Asn Asp Thr Val Ile Arg

115 120 125

Glu Phe Val Glu Lys Val Ile Gly Ile Trp Pro Glu Leu Thr Arg Arg

130 135 140

Tyr Lys Gly Pro Gly Gln Cys Ser Glu Cys Ala Asn Ser Phe Ile Pro

145 150 155 160

Ile Asn Arg Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr

165 170 175

Tyr Trp Asp Ser Tyr Trp Ile Val Glu Gly Leu Leu Arg Thr Gly Gly

180 185 190

Ala Phe Thr Gln Ile Ser Lys Asn Thr Ile Glu Asn Phe Leu Asp Leu

195 200 205

Val Glu Thr Ile Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Arg

210 215 220

Asn Arg Ser Gln Leu Pro Val Leu Ser Gln Met Val Arg Val Tyr Val

225 230 235 240

Glu His Thr Asn Asp Thr Ser Ile Leu Glu Arg Ala Val Pro Leu Leu

245 250 255

Ile Lys Glu His Gln Phe Phe Val Asn Asn Arg Ser Val Glu Val Thr

260 265 270

Ser Arg Ser Gly Gln Thr Tyr Thr Leu Asn Arg Tyr Ala Val Val Asn

275 280 285

Thr Gln Pro Arg Pro Glu Ser Phe Arg Glu Asp Tyr Ile Thr Ala Asn

290 295 300

Asn Arg Ser Tyr Tyr Ala Ala Ser Gly Ile Ile Tyr Pro Glu Ala Arg

305 310 315 320

Gln Leu Asn Glu Ser Glu Lys Ala Glu Leu Tyr Ser His Leu Ala Ser

325 330 335

Gly Ala Glu Ser Gly Trp Asp Tyr Ser Thr Arg Trp Leu Ala Lys Pro

340 345 350

Asn Asp Ala Ala Lys Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Thr

355 360 365

Asn Asn Met Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Ala Asn Glu

370 375 380

Leu Ala Ile Ser Glu Phe Leu Asn Gln Thr Gly Asn Ala Thr Gly Ala

385 390 395 400

Ala Glu Trp Lys Arg Thr Ala Glu Arg Arg Ser Glu Ala Met Phe Ala

405 410 415

Leu Met Trp Asn Glu Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Met Thr

420 425 430

Ser Lys Glu Gln Asn Ile Tyr Ile Pro Leu Asp Asp Asp Ala Thr Ala

435 440 445

Phe Glu Gln Thr Thr Ala Pro Ala Gly Lys Gln Val Leu Phe His Val

450 455 460

Ala Gln Phe Tyr Pro Phe Trp Thr Gly Ala Ala Pro Ala Gln Leu Arg

465 470 475 480

Asp Asn Pro Leu Ala Val Gln Arg Ala Tyr Ser Arg Val Asp Ala Tyr

485 490 495

Leu Gly Leu Lys Gly Gly Ala Ile Pro Ala Thr Asn Leu Arg Thr Gly

500 505 510

Gln Gln Trp Asp Glu Pro Asn Val Trp Pro Pro Leu Met His Ile Leu

515 520 525

Met Glu Gly Leu Thr Arg Val Pro Ala Thr Phe Gly Glu Ser Asp Pro

530 535 540

Ala Trp Leu Gly Val Gln Asp Leu Ala Leu Arg Leu Gly Gln Arg Tyr

545 550 555 560

Leu Asp Ser Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser

565 570 575

Gln Thr Pro Gln Leu Gln Gly Leu Thr Pro Glu Asp Thr Gly Ile Met

580 585 590

Phe Glu Lys Tyr Gly Asp Asn Ser Thr Asn Val Ala Gly Ala Gly Gly

595 600 605

Glu Tyr Glu Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Ile

610 615 620

Trp Val Ala Asp Gln Phe Ser Thr Arg Leu Lys Arg Pro Asn Cys Gly

625 630 635 640

Asn Ile Thr Ala Ala Asn Val His Pro Ser Gly Gly Arg Arg Lys Arg

645 650 655

Ser Leu Glu Gln Arg Ser Ala Leu Glu Met His Ser Ser Asp Ala Ala

660 665 670

Trp Ile Lys Arg Phe Gly Arg Lys Ala Ala Lys Arg Ser Leu Gly Arg

675 680 685

Thr Ala Gly Leu Gly Gly Gly Ala Val

690 695

<210> 73

<211> 646

<212> PRT

<213> 夏季斑枯病菌(Magnaporthiopsis poae)

<400> 73

Met Ala Pro Ser Trp Lys Leu Ala Ala Ala Val Thr Ala Trp Ser Leu

1 5 10 15

Ala Val Asn Ala Leu Tyr Ile Asn Gly Thr Glu Ile Thr Pro Cys Asp

20 25 30

Ser Pro Ile Tyr Cys Arg Gly Glu Leu Leu Lys Gln Val Glu Leu Ala

35 40 45

Arg Pro Phe Ala Asp Ser Lys Thr Phe Val Asp Met Pro Thr Ile Lys

50 55 60

Pro Val Asp Glu Val Ile Ala Ala Phe Asn Lys Leu Gln Lys Pro Leu

65 70 75 80

Ser Asn Asn Thr Glu Leu Gln Asn Phe Leu Arg Glu Asn Phe Ala Gln

85 90 95

Ala Gly Gly Glu Leu Glu Glu Val Pro Asn Ser Glu Leu Thr Thr Asn

100 105 110

Pro Val Phe Leu Lys Lys Leu Asn Asp Thr Val Ile Arg Glu Phe Val

115 120 125

Glu Lys Val Ile Asp Ile Trp Pro Glu Leu Thr Arg Arg Tyr Lys Gly

130 135 140

Pro Gly Gln Cys Ser Asp Cys Ala Asn Ser Phe Ile Pro Val Asn Arg

145 150 155 160

Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp

165 170 175

Ser Tyr Trp Ile Val Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe Thr

180 185 190

Gln Ile Ser Lys Asn Thr Ile Glu Asn Phe Leu Asp Leu Val Glu Thr

195 200 205

Ile Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Arg Asn Arg Ser

210 215 220

Gln Pro Pro Leu Leu Ser Gln Met Val Arg Ile Tyr Ile Glu His Thr

225 230 235 240

Asn Asp Thr Ser Ile Leu Glu Arg Ala Val Pro Leu Leu Ile Lys Glu

245 250 255

His Glu Phe Phe Val Lys Asn Arg Ser Ile Glu Val Thr Ser Arg Ser

260 265 270

Gly Gln Lys Tyr Thr Leu Asn Arg Tyr Ser Val Val Asn Thr Gln Pro

275 280 285

Arg Pro Glu Ser Phe Arg Glu Asp Tyr Ile Thr Ala Asn Asn Arg Ser

290 295 300

Tyr Tyr Ala Ala Ser Gly Ile Ile Tyr Pro Glu Gly Arg Gln Leu Asn

305 310 315 320

Glu Ser Glu Lys Ala Glu Leu Tyr Ser Tyr Leu Ala Ser Gly Ala Glu

325 330 335

Ser Gly Trp Asp Tyr Cys Ala Arg Trp Leu Arg Lys Pro Asp Asp Ala

340 345 350

Ala Lys Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Thr Asn Asn Met

355 360 365

Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Ala Asn Glu Leu Thr Ile

370 375 380

Ser Gln Phe Leu Asn Arg Thr Gly Asn Ala Thr Gly Ala Ala Glu Trp

385 390 395 400

Lys Gly Leu Ala Glu Arg Arg Ser Glu Ala Met Phe Ala Leu Met Trp

405 410 415

Asn Glu Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Thr Thr Ser Gly Glu

420 425 430

Gln Asn Ile Tyr Val Pro Leu Asp Asp Asp Ala Thr Ala Leu Glu Gln

435 440 445

Ile Thr Ala Pro Ala Gly Lys Gln Val Leu Phe His Val Ala Gln Phe

450 455 460

Tyr Pro Phe Trp Thr Gly Ala Ala Pro Lys Gln Leu Arg Asp Asn Pro

465 470 475 480

Leu Ala Val Leu Gln Ala Tyr Ser Arg Val Asp Ala Tyr Leu Lys Leu

485 490 495

Lys Gly Gly Ala Ile Pro Ala Thr Asn Leu Arg Thr Gly Gln Gln Trp

500 505 510

Asp Glu Pro Asn Val Trp Pro Pro Leu Met His Val Leu Met Glu Gly

515 520 525

Leu Thr Arg Val Pro Ala Thr Phe Gly Glu Ser Asp Pro Ala Trp Leu

530 535 540

Gly Tyr Gly Asp Asn Ser Thr Asn Val Ala Gly Ala Gly Gly Glu Tyr

545 550 555 560

Glu Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Ile Trp Val

565 570 575

Ala Asp Gln Phe Ser Thr Arg Leu Lys Arg Pro Asn Cys Gly Asp Ile

580 585 590

Thr Ala Ala Lys Val His Ala Ser Gly Gly Arg Lys Gln Ser Leu Ala

595 600 605

Gln Arg Ser Ala Leu Glu Met His Pro Ala Asp Ala Gly Trp Thr Lys

610 615 620

Met Phe Gly Arg Lys Ala Ala Lys Arg Ser Leu Ala Arg Thr Val Glu

625 630 635 640

Leu Gly Asn Gly Met Val

645

<210> 74

<211> 694

<212> PRT

<213> 嗜热毁丝霉(Thermothelomyces thermophila)

<400> 74

Met Ala Leu Arg His Ala Ala Thr Ala Ala Leu Ala Gly Leu Ser Ser

1 5 10 15

Ser Ala Ala Ala Leu Tyr Ile Asn Gly Ser Val Thr Ala Pro Cys Asp

20 25 30

Ser Pro Ile Tyr Cys His Gly Glu Leu Leu Lys Gly Val Glu Leu Ala

35 40 45

His Pro Phe Val Asp Ser Lys Thr Phe Val Asp Met Pro Thr Leu Lys

50 55 60

Pro Val Asp Glu Val Leu Ala Ala Phe Ser Lys Leu Arg Gln Pro Leu

65 70 75 80

Ser Asn Asn Ser Glu Leu Asn Asn Phe Leu Ala Glu Tyr Phe Ala Pro

85 90 95

Ala Gly His Glu Leu Glu Glu Val Pro Lys Gly Glu Leu Gln Ile Asp

100 105 110

Pro Lys Phe Leu Asn Lys Leu Glu Asp Arg Thr Ile Lys Glu Phe Val

115 120 125

Ser Lys Val Ile Asp Ile Trp Pro Asp Leu Thr Arg Arg Tyr Ala Gly

130 135 140

Pro Gly Asp Cys Ser Gly Cys Ala Asn Ser Phe Ile Pro Val Asn Arg

145 150 155 160

Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp

165 170 175

Ser Tyr Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe Thr

180 185 190

Gln Ile Ser Lys Asn Ile Ile Glu Asn Phe Leu Asp Phe Ile Asp Thr

195 200 205

Ile Gly Phe Ile Pro Asn Gly Ala Arg Ile Tyr Tyr Leu Asn Arg Ser

210 215 220

Gln Pro Pro Leu Leu Thr Arg Met Val Lys Ser Tyr Val Asp Tyr Thr

225 230 235 240

Asn Asp Thr Ser Ile Leu Glu Arg Ala Leu Pro Leu Leu Ile Lys Glu

245 250 255

His Asp Phe Phe Thr Asn Asn Arg Ser Val Ser Val Thr Ala Ser Asn

260 265 270

Gly Lys Thr Tyr Thr Leu His Arg Tyr His Val Glu Asn Asn Gln Pro

275 280 285

Arg Pro Glu Ser Tyr Arg Glu Asp Tyr Ile Thr Ala Asn Asn Gly Ser

290 295 300

Tyr Tyr Ala Ala Ser Gly Ile Ile Tyr Pro Val Lys Thr Pro Leu Asn

305 310 315 320

Glu Thr Glu Lys Ala Val Leu Tyr Ser Asn Leu Ala Ser Gly Ala Glu

325 330 335

Ser Gly Trp Asp Tyr Thr Ala Arg Trp Leu Arg Val Pro Asp Asp Ala

340 345 350

Ala Arg Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Val Arg Glu Met

355 360 365

Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Glu Asn Glu Val Ile Ile

370 375 380

Ala Glu Tyr Leu Glu Lys Ala Gly Asn Ser Ser Glu Ala Lys Arg Phe

385 390 395 400

Ala Ser Ala Ala Lys Gln Arg Ser Glu Ala Met Tyr Asn Leu Met Trp

405 410 415

Asn Ala Thr His Trp Ser Tyr Phe Asp Tyr Asn Leu Thr Ser Asn Ala

420 425 430

Gln Asn Ile Phe Val Pro Ala Asp Glu Asp Thr Ala Ser Phe Asp Arg

435 440 445

Tyr Ala Ala Pro Pro Gly Gln Gln Val Leu Phe His Val Ala Gln Leu

450 455 460

Tyr Pro Phe Trp Thr Gly Ala Ala Pro Ala His Leu Lys Ser Asn Pro

465 470 475 480

Leu Ala Val Gln Lys Ala Tyr Ala Arg Val Ser Arg Arg Leu Asp Thr

485 490 495

Lys Lys Gly Ala Ile Ala Ala Thr Asn Tyr Arg Thr Gly Gln Gln Trp

500 505 510

Asp Gln Pro Asn Val Trp Pro Pro Leu Gln His Val Leu Met Gln Gly

515 520 525

Leu Leu Asn Thr Pro Ala Thr Phe Gly Glu Ser Asp Pro Ala Tyr Gln

530 535 540

Gly Val Gln Lys Leu Ala Leu Arg Leu Ala Gln Arg Tyr Leu Asp Ser

545 550 555 560

Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Asp Phe Pro

565 570 575

Gln Leu Gln Gly Val Ser Pro Asp Ala Thr Gly Ile Met Phe Glu Lys

580 585 590

Tyr Ala Asp Ser Ala Thr Asn Val Ala Gly Gly Gly Gly Glu Tyr Glu

595 600 605

Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Ile Trp Ala Ala

610 615 620

Asp Val Phe Gly Asn Lys Leu Lys Arg Pro Asp Cys Gly Asn Ile Thr

625 630 635 640

Ala Ala His Thr His Ser Glu Ala Lys Arg Ser Leu Gly Asp Gly Gly

645 650 655

Leu Ala Arg Arg Ala Val Glu Leu Asp Pro Trp Asp Ala Ala Trp Thr

660 665 670

Lys Met Phe Gly Arg Ser Lys Leu Arg Arg Arg Glu Ala Glu Asp Val

675 680 685

Arg Lys Arg Trp Ser Ser

690

<210> 75

<211> 680

<212> PRT

<213> 若虫炭疽菌(Colletotrichum nymphaeae)

<400> 75

Met Val Asn Phe Ala Leu Ala Leu Ala Ala Val Ala Val Leu Pro Arg

1 5 10 15

Thr Ala Gln Ala Leu Tyr Glu Asn Gly Ser Val Val Ala Pro Cys Asp

20 25 30

Ser Pro Ile Tyr Cys His Gly Asp Ile Leu Lys Glu Val Glu Leu Ala

35 40 45

Arg Pro Phe Ser Asp Ser Lys Thr Phe Val Asp Met Pro Ala Ile Lys

50 55 60

Ser Leu Glu Glu Ile Gln Ala Ala Phe Asn Asn Leu Ser Lys Pro Leu

65 70 75 80

Ser Asn Asn Thr Ala Leu Asn Asp Phe Leu Arg Ala Asn Phe Ala Ala

85 90 95

Ala Gly Gly Glu Leu Lys Glu Val Pro Lys Asp Gln Leu Lys Thr Asp

100 105 110

Pro Lys Phe Leu Asp Lys Ile Asn Asp Thr Val Ile Lys Glu Phe Ser

115 120 125

Gln Lys Val Ile Ala Ile Trp Pro Asp Leu Thr Arg Thr Tyr Ala Gly

130 135 140

Ser Thr Ser Asn Cys Thr Thr Cys Pro Asn Ser Phe Ile Pro Val Asn

145 150 155 160

His Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp

165 170 175

Asp Ser Phe Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe

180 185 190

Thr Glu Ile Ser Lys Asn Val Ile Glu Asn Phe Leu Asp Leu Val Glu

195 200 205

Lys Leu Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Leu Asn Arg

210 215 220

Ser Gln Pro Pro Leu Leu Ala Gln Met Val Arg Leu Tyr Val Glu Tyr

225 230 235 240

Thr Ser Asp Arg Ser Ile Leu Glu Arg Ala Ile Pro Leu Leu Ile Lys

245 250 255

Glu His Glu Phe Trp Thr Thr Asn Arg Thr Val Glu Val Asn Lys Asn

260 265 270

Gly Thr Thr Tyr Thr Leu Asn Gln Tyr Asn Val Thr Asn Thr Gln Pro

275 280 285

Arg Pro Glu Ser Tyr Arg Glu Asp Tyr Val Thr Ala Ser Asn Ala Ser

290 295 300

Tyr Tyr Ser Thr Asn Gly Ile Ile Tyr Pro Glu Val Glu Lys Leu Asn

305 310 315 320

Asp Thr Glu Lys Ala Arg Val Tyr Ala Asn Leu Ala Ser Gly Ala Glu

325 330 335

Ser Gly Trp Asp Tyr Ser Ser Arg Trp Leu Ser Arg Pro Ala Asp Ala

340 345 350

Ala Arg Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Val Leu Glu Thr

355 360 365

Val Pro Val Glu Leu Asn Ser Ile Leu Tyr Trp Asn Glu Met Thr Ile

370 375 380

Ala Ala Leu Leu Asn Ser Thr Asn Asn Gly Thr Gly Ala Val Ala Trp

385 390 395 400

Thr Glu Leu Ala Thr Lys Arg Ser Gln Ala Met Tyr Asp Leu Met Trp

405 410 415

Asn Ser Thr Leu Ser Ser Tyr Phe Asp Tyr Asn Val Thr Ser Gly Gly

420 425 430

Gln Asn Ile Tyr Ile Pro Lys Asp Asp Asp Ala Ser Thr Leu Glu Thr

435 440 445

Asn Thr Ala Glu Ala Glu Gln Gln Val Leu Phe Ser Val Ser Gln Phe

450 455 460

Tyr Pro Phe Trp Thr Gly Ala Ala Pro Ser His Leu Lys Asn Asn Pro

465 470 475 480

Tyr Ala Val Lys Gln Ala Tyr Gln Arg Val Glu Lys Tyr Leu Asp Ile

485 490 495

Lys Lys Gly Gly Ile Pro Ala Thr Asn Leu Lys Thr Gly Gln Gln Trp

500 505 510

Asp Gln Pro Asn Val Trp Pro Pro Leu Met His Val Leu Met Glu Gly

515 520 525

Leu Arg Arg Thr Pro Ala Thr Phe Gly Glu Ser Asp Pro Ala Trp Arg

530 535 540

Asp Val Gln Ser Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp Ser

545 550 555 560

Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu Thr Pro

565 570 575

Gln Leu Gln Gly Leu Thr Ala Gln Ser Val Gly Thr Met Phe Glu Lys

580 585 590

Tyr Gly Asp Asn Ser Thr Asn Val Ala Gly Gly Gly Gly Glu Tyr Glu

595 600 605

Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Leu Trp Ala Val

610 615 620

Asp Val Phe Gly Asn Asp Leu Lys Arg Pro Asp Cys Gly Asn Ile Thr

625 630 635 640

Ala Ala Asn Val His Pro Ala Lys Arg Ser Leu Pro Gln Arg Ala Val

645 650 655

Glu Leu Asp Ser Tyr Asp Ala Ser Trp Thr Lys Lys Phe Gly Arg Arg

660 665 670

Ala Glu Glu Ala Ala Lys Arg Val

675 680

<210> 76

<211> 680

<212> PRT

<213> 兰花炭疽菌(Colletotrichum orchidophilum)

<400> 76

Met Val Asn Phe Ala Leu Ala Leu Ala Ala Ile Ala Val Leu Pro Gln

1 5 10 15

Thr Ala Gln Ala Leu Tyr Glu Asn Gly Ser Ile Val Ala Pro Cys Asp

20 25 30

Ser Pro Ile Tyr Cys His Gly Asp Ile Leu Arg Glu Val Glu Leu Ala

35 40 45

Arg Pro Phe Ser Asp Ser Lys Thr Phe Val Asp Met Pro Ala Ile Lys

50 55 60

Ser Leu Glu Glu Ile Gln Ala Ala Phe Asn Asn Leu Ser Lys Pro Leu

65 70 75 80

Ser Asn Asn Thr Ala Leu Asn Asp Phe Leu Arg Ala Asn Phe Ala Ala

85 90 95

Ala Gly Gly Glu Leu Thr Glu Val Pro Lys Asp Gln Leu Lys Thr Asp

100 105 110

Pro Lys Phe Leu Asp Asn Ile Asn Asp Thr Val Ile Lys Glu Phe Ser

115 120 125

Gln Lys Val Ile Ala Ile Trp Pro Asp Leu Thr Arg Thr Tyr Thr Gly

130 135 140

Ser Ala Ser Asn Cys Thr Thr Cys Pro Asn Ser Phe Ile Pro Val Asn

145 150 155 160

His Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp

165 170 175

Asp Ser Phe Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe

180 185 190

Thr Glu Ile Ser Lys Asn Val Ile Glu Asn Phe Leu Asp Leu Val Asp

195 200 205

Lys Leu Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Leu Asn Arg

210 215 220

Ser Gln Pro Pro Leu Leu Ala Gln Met Val Arg Leu Tyr Val Glu Tyr

225 230 235 240

Thr Asn Asp Arg Ser Ile Leu Gly Arg Ala Ile Pro Leu Leu Ile Lys

245 250 255

Glu His Glu Phe Trp Met Thr Asn Arg Thr Val Glu Val Asn Arg Asn

260 265 270

Gly Thr Thr Tyr Ser Leu Asn Gln Tyr Asn Val Thr Asn Thr Gln Pro

275 280 285

Arg Pro Glu Ser Tyr Arg Glu Asp Tyr Val Thr Ala Ser Asn Ala Ser

290 295 300

Tyr Tyr Ser Thr Ser Gly Ile Ile Tyr Pro Glu Val Glu Lys Leu Asn

305 310 315 320

Asp Thr Glu Lys Ala Arg Leu Tyr Ala Asn Leu Ala Ser Gly Ala Glu

325 330 335

Ser Gly Trp Asp Tyr Thr Ser Arg Trp Leu Ser Arg Pro Gly Asp Ala

340 345 350

Ala Arg Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Val Leu Glu Thr

355 360 365

Val Pro Val Glu Leu Asn Ser Ile Leu Tyr Trp Asn Glu Met Thr Ile

370 375 380

Ala Ala Leu Leu Asn Ser Thr Gln Asn Gly Thr Gly Ala Leu Thr Trp

385 390 395 400

Thr Glu Leu Ala Val Lys Arg Ser Gln Ala Met Tyr Asp Leu Met Trp

405 410 415

Asn Ser Thr Leu Ser Ser Tyr Phe Asp Tyr Asn Val Ser Ser Gly Ala

420 425 430

Gln Asn Ile Tyr Ile Pro Arg Asp Asp Asp Ala Thr Glu Val Glu Thr

435 440 445

Asn Thr Ala Gly Ser Gln Gln Gln Val Leu Phe Ser Val Ser Gln Phe

450 455 460

Tyr Pro Phe Trp Thr Gly Ala Ala Pro Ser His Leu Lys Asn Asn Pro

465 470 475 480

Tyr Ala Val Lys Gln Ala Tyr Gln Arg Val Glu Lys Tyr Leu Asp Asn

485 490 495

Lys Lys Gly Gly Ile Pro Ala Thr Asn Leu Lys Thr Gly Gln Gln Trp

500 505 510

Asp Gln Pro Asn Val Trp Pro Pro Leu Met His Val Leu Met Glu Gly

515 520 525

Leu Arg Lys Thr Pro Ala Thr Phe Gly Glu Ser Asp Pro Ala Trp Arg

530 535 540

Asp Val Gln Asn Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp Ser

545 550 555 560

Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu Thr Pro

565 570 575

Gln Leu Gln Gly Leu Thr Ala Gln Ser Val Gly Thr Met Phe Glu Lys

580 585 590

Tyr Gly Asp Asn Ser Thr Asn Val Ala Gly Gly Gly Gly Glu Tyr Glu

595 600 605

Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Leu Trp Ala Val

610 615 620

Asp Val Phe Gly Asn Gly Leu Lys Arg Pro Asp Cys Gly Asn Ile Thr

625 630 635 640

Ala Ala Asn Val His Pro Ala Lys Arg Ser Leu Ser Gln Arg Ala Val

645 650 655

Glu Leu Asp Gly Tyr Asp Ala Ala Trp Ile Lys Lys Phe Gly Arg Arg

660 665 670

Ala Glu Glu Ala Ala Arg Arg Leu

675 680

<210> 77

<211> 685

<212> PRT

<213> 木生锥毛壳(Coniochaeta ligniaria)

<400> 77

Met Ala Leu Ser Arg Leu Met Ala Ala Leu Ala Ala Ser Ile Ser Val

1 5 10 15

Val Asn Ala Leu Tyr Val Asn Gly Ser Val Thr Ala Pro Cys Asp Ser

20 25 30

Pro Leu Tyr Cys Gln Gly Asp Ile Leu Lys Ala Ile Glu Leu Ala Gln

35 40 45

Pro Phe Ser Asp Ser Lys Thr Phe Val Asp Met Pro Thr Ile Lys Pro

50 55 60

Leu Asp Glu Val Ile Ala Ala Phe Asn Lys Leu Thr Leu Pro Ile Thr

65 70 75 80

Asn Asn Thr Glu Leu Asn Asp Phe Leu Ala Ala Asn Phe Ala Pro Ala

85 90 95

Gly Gly Glu Leu Glu Thr Val Pro Thr Asn Gln Leu Gln Thr Asp Pro

100 105 110

Lys Phe Leu Asp Lys Leu Asn Asp Thr Val Ile Arg Glu Phe Ser Gln

115 120 125

Ala Val Ile Lys Ile Trp Pro Asp Leu Thr Arg Gln Tyr Ala Gly Pro

130 135 140

Ser Asn Cys Thr Gly Cys Val Asp Ser Phe Ile Pro Val Asn Arg Thr

145 150 155 160

Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp Ser

165 170 175

Tyr Trp Ile Val Glu Gly Leu Leu Arg Thr Gly Gly Asn Phe Thr Ala

180 185 190

Ile Ala Arg Asn Thr Ile Glu Asn Phe Leu Asp Leu Val Glu Thr Ile

195 200 205

Gly Phe Val Pro Asn Gly Ala Arg Leu Tyr Tyr Leu Asn Arg Ser Gln

210 215 220

Pro Pro Leu Leu Ser Lys Met Val Arg Ser Tyr Val Asp Tyr Thr Asn

225 230 235 240

Asp Thr Ser Ile Leu Glu Arg Ala Val Pro Leu Leu Ile Lys Glu His

245 250 255

Gln Phe Trp Gln Thr Asn Arg Thr Val Val Val Thr Ala Ala Asp Gly

260 265 270

Lys Asn Tyr Thr Leu Asn Arg Tyr His Val Asn Asn Asn Gln Pro Arg

275 280 285

Pro Glu Ser Phe Arg Glu Asp Tyr Ile Thr Ala Asn Asn His Ser Tyr

290 295 300

Tyr Ala Ala Ser Gly Ile Ile Tyr Pro Glu Gly Lys Ala Leu Asn Asp

305 310 315 320

Ser Glu Lys Gln Gln Leu Tyr Ala Asn Leu Ala Thr Gly Ala Glu Ser

325 330 335

Gly Trp Asp Tyr Gly Thr Arg Trp Leu Ala Arg Pro Asn Asp Ala Ala

340 345 350

Lys Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Val Val Asn Ile Val

355 360 365

Pro Val Asp Leu Asn Ser Ile Leu Tyr Gly Ala Glu Ser Asn Ile Ala

370 375 380

His Phe Leu Asn Gln Thr Gly Lys Ser Thr Glu Ala Tyr Lys Trp Ala

385 390 395 400

Asn Leu Ala Ala Gln Arg Ser Glu Ala Met Phe Ala Leu Met Trp Asn

405 410 415

Ser Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Leu Thr Ser Asn Ala Gln

420 425 430

Asn Ile Tyr Ile Pro Ala Asp Asp Asp Ala Ile Thr Ala Glu Thr Ser

435 440 445

Gly Ala Pro Ala Gly Tyr Gln Ile Leu Phe Asp Val Ala Gln Leu Tyr

450 455 460

Pro Phe Trp Thr Gly Ala Ala Pro Asp Gln Leu Lys Asp Asn Pro Leu

465 470 475 480

Ala Val Lys Leu Ala Phe Ser Arg Val Ser Ser Met Leu Asp Arg Lys

485 490 495

Ala Gly Gly Ile Pro Ala Thr Asn Phe Gln Thr Gly Gln Gln Trp Asp

500 505 510

Gln Pro Asn Val Trp Pro Pro Leu Met His Val Leu Met Ala Gly Leu

515 520 525

Leu Asn Thr Pro Ala Thr Phe Gly Glu Asp Asp Pro Ser Tyr Gln Asp

530 535 540

Val Gln Ala Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp Ser Thr

545 550 555 560

Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Ala Thr Pro Lys

565 570 575

Leu Gln Gly Ala Gly Ala Asp Ala Val Gly Thr Met Phe Glu Lys Tyr

580 585 590

Ala Asp Asn Ala Thr Asn Val Ala Gly Gly Gly Gly Glu Tyr Thr Val

595 600 605

Val Glu Gly Phe Gly Trp Ser Asn Gly Val Leu Leu Trp Ala Ala Asp

610 615 620

Thr Phe Ala Asp Lys Leu Lys Arg Pro Asp Cys Gly Asn Ile Thr Ala

625 630 635 640

Ala Asn Val Asn Pro Gly Lys Arg Ser Val Arg Ser Ala Val Glu Leu

645 650 655

Asp Ala Arg Asp Ala Lys Trp Thr Lys Met Phe Gly Ser Val Ala Arg

660 665 670

Arg Gln Ala Glu Lys Arg Asp Ala Glu Lys Arg Thr Tyr

675 680 685

<210> 78

<211> 695

<212> PRT

<213> 土生梭抱壳霉(Thielavia terrestris)

<400> 78

Met Ala Pro Arg Ser Phe Val Ala Ala Ala Ala Leu Ala Gly Leu Ile

1 5 10 15

Ser Ser Ala Ser Ala Leu Tyr Ile Asn Gly Ser Val Thr Ala Pro Cys

20 25 30

Asp Ser Pro Leu Tyr Cys His Gly Glu Ile Leu Lys Ala Ile Glu Leu

35 40 45

Ala His Pro Phe Thr Asp Ser Lys Thr Phe Val Asp Met Pro Thr Ile

50 55 60

Arg Pro Leu Asp Glu Val Ile Ala Ala Phe Asn Arg Leu Ser Gln Pro

65 70 75 80

Leu Ser Asn Asn Ser Glu Leu Asn Ala Phe Leu Ala Ala Asn Phe Ala

85 90 95

Pro Ala Gly Gly Glu Leu Glu Ala Val Pro Arg Asp Gln Leu His Thr

100 105 110

Glu Pro Ser Phe Leu Asn Lys Leu Asp Asp Thr Val Ile Lys Glu Phe

115 120 125

Val Ala Lys Val Ile Asp Ile Trp Pro Asp Leu Thr Arg Arg Tyr Gly

130 135 140

Gly Pro Gly Asn Cys Thr Ala Cys Ala Asn Ser Phe Ile Pro Val Asn

145 150 155 160

Arg Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp

165 170 175

Asp Ser Tyr Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe

180 185 190

Thr Glu Ile Ser Lys Asn Ile Ile Glu Asn Phe Leu Asp Phe Val Glu

195 200 205

Thr Ile Gly Phe Ile Pro Asn Gly Ala Arg Ile Tyr Tyr Leu Asp Arg

210 215 220

Ser Gln Pro Pro Leu Leu Ala Arg Met Val Arg Ser Tyr Val Asp Tyr

225 230 235 240

Thr Asn Asp Thr Ser Ile Leu Asp Arg Ala Leu Pro Leu Leu Ile Lys

245 250 255

Glu His Glu Phe Trp Ser Thr Asn Arg Ser Val Ser Ile Lys Ala Pro

260 265 270

Asn Gly Lys Thr Tyr Thr Leu Asn Arg Tyr Tyr Val Asn Asn Asn Gln

275 280 285

Pro Arg Pro Glu Ser Phe Arg Glu Asp Tyr Ile Thr Ala Asn Asn Gly

290 295 300

Ser Tyr Tyr Ala Ala Ser Gly Ile Ile Tyr Pro Val Asn Thr Pro Leu

305 310 315 320

Asn Asp Thr Glu Lys Ala Glu Leu Tyr Ala Asn Leu Ala Ser Gly Ala

325 330 335

Glu Thr Gly Trp Asp Tyr Ser Thr Arg Trp Leu Lys Asn Pro Asn Asp

340 345 350

Ala Ala Lys Asp Ile Tyr Phe Pro Leu Arg Ser Leu Asn Val Arg Gly

355 360 365

Thr Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Glu Asn Glu Val Ile

370 375 380

Ile Ser Gln Tyr Leu Lys Arg Ala Gly Asn Asn Ser Glu Ala Glu Arg

385 390 395 400

Trp Ala Tyr Ala Ala Ser Gln Arg Ser Glu Ala Met Phe Glu Leu Met

405 410 415

Trp Asn Ala Thr His Trp Ser Tyr Phe Asp Tyr Asn Leu Thr Ser Asn

420 425 430

Ser Gln Arg Ile Phe Val Pro Val Asp Asp Asp Ala Thr Ala Ala Glu

435 440 445

Arg Ala Gly Ala Pro Arg Gly Gln Gln Val Leu Phe Asn Ile Gly Gln

450 455 460

Phe Tyr Pro Phe Trp Thr Gly Ala Ala Pro Ala Gln Leu Lys Asn Asn

465 470 475 480

Pro Leu Ala Val Gln Gln Ala Tyr Ala Arg Val Ala Arg Met Leu Asp

485 490 495

Glu Asn Ala Gly Gly Ile Pro Ala Thr Asn Phe Val Thr Gly Gln Gln

500 505 510

Trp Asp Gln Pro Asn Val Trp Pro Pro Leu Gln His Val Leu Met Glu

515 520 525

Gly Leu Leu Asn Thr Pro Pro Thr Phe Gly Asp Ala Asp Pro Ala Tyr

530 535 540

Gln Ser Val Arg Ala Leu Ala Leu Arg Leu Ala Gln Arg Tyr Leu Asp

545 550 555 560

Ser Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Gln Thr

565 570 575

Pro Gln Leu Gln Gly Val Ala Pro Gly Ala Glu Gly Ile Met Phe Glu

580 585 590

Lys Tyr Ala Asp Asn Ser Thr Asn Val Ala Gly Ser Gly Gly Glu Tyr

595 600 605

Glu Val Val Glu Gly Phe Gly Trp Ser Asn Gly Val Leu Ile Trp Ala

610 615 620

Ala Asp Val Phe Gly Ala Gln Leu Lys Arg Pro Asp Cys Gly Asn Ile

625 630 635 640

Thr Ala Ala His Thr Ser Gly Ser Gly Ala Gln Lys Arg Ser Gly Gly

645 650 655

Ser Leu Ala Arg Arg Ala Val Glu Leu Asp Pro Trp Asp Ala Ala Trp

660 665 670

Thr Lys Met Phe Gly Arg Ser Ala Leu Lys Lys Arg Glu Asp Val Arg

675 680 685

Lys Arg Trp Leu Leu Ala Ala

690 695

<210> 79

<211> 682

<212> PRT

<213> 足马杜拉分枝菌(Madurella mycetomatis)

<400> 79

Met Val Pro Arg Leu Ser Val Thr Ala Ala Leu Ala Ala Phe Ala Ser

1 5 10 15

Ser Ala Ala Ala Leu Tyr Ile Asn Gly Ser Val Ile Ala Pro Cys Asp

20 25 30

Ser Pro Leu Tyr Cys Gln Gly Glu Ile Leu His Ala Ile Glu Leu Ala

35 40 45

Arg Pro Phe Ser Asp Ser Lys Thr Phe Val Asp Met Pro Thr Ile Arg

50 55 60

Pro Leu Glu Glu Val Ile Ala Ala Phe Ser Arg Leu Thr Gln Pro Leu

65 70 75 80

Ser Asn Asn Ser Glu Leu Asn Asp Phe Leu Ala Ala Asn Phe Ala Pro

85 90 95

Ala Gly Gly Glu Leu Glu Glu Val Pro Glu Asp Glu Leu His Thr Asp

100 105 110

Pro Val Phe Leu Asp Asn Leu Asp Asp Ala Val Ile Arg Glu Phe Val

115 120 125

Gly Gln Val Ile Asp Ile Trp Pro Asp Leu Thr Arg Arg Tyr Ala Gly

130 135 140

Pro Gly Asn Cys Ser Glu Cys Ala Asn Ser Phe Ile Pro Val Asn Arg

145 150 155 160

Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp

165 170 175

Ser Tyr Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe Thr

180 185 190

Glu Ile Ser Arg Asn Ile Ile Glu Asn Phe Leu Asp Phe Val Glu Thr

195 200 205

Ile Gly His Val Pro Asn Gly Ala Arg Val Tyr Tyr Leu Asn Arg Ser

210 215 220

Gln Pro Pro Leu Leu Thr Leu Met Val Lys Thr Tyr Val Asp Tyr Thr

225 230 235 240

Asn Asp Thr Ser Ile Leu Glu Arg Ala Leu Pro Leu Leu Ile Arg Glu

245 250 255

His Glu Phe Trp Thr Asn Asn Arg Thr Val Asp Val Thr Ala Ser Asp

260 265 270

Gly Thr Thr Tyr Arg Leu Asn Arg Tyr Asp Val Ser Asn Thr Gln Pro

275 280 285

Arg Pro Glu Ser Tyr Arg Glu Asp Trp Val Thr Ala Asn Asn Ala Ser

290 295 300

Tyr Tyr Ala Ala Ser Gly Ile Ile Tyr Pro Val Arg Thr Pro Leu Asn

305 310 315 320

Glu Ser Glu Ile Ala Glu Leu Tyr Ser Asn Leu Ala Ser Gly Ala Glu

325 330 335

Ser Gly Trp Asp Tyr Thr Ser Arg Trp Leu Met Arg Pro Asn Asp Ala

340 345 350

Ala Arg Asp Val Tyr Phe Pro Leu Arg His Leu Asn Val Arg Asn Met

355 360 365

Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Gln Asn Glu Val Ile Ile

370 375 380

Ser Gly Tyr Leu Glu Gln Ala Gly Asn Ser Thr Glu Ala Glu Arg Phe

385 390 395 400

Ala Ser Ala Ala Arg Glu Arg Ser Glu Ala Met Tyr Ala Leu Met Trp

405 410 415

Asn Glu Thr His Trp Ser Tyr Phe Asp Tyr Asn Leu Thr Ser Asn Glu

420 425 430

Gln Arg Ile Phe Val Pro Thr Asp Ala Asn Ala Thr Thr Ala Glu Leu

435 440 445

Ala Gly Ala Pro Glu Gly Tyr Gln Val Leu Phe Asp Ile Ala Gln Leu

450 455 460

Tyr Pro Phe Trp Thr Gly Ala Ala Pro Asp Gln Leu Lys Asn Asn Pro

465 470 475 480

Leu Ala Val Gln Gln Ala Tyr Ser Arg Val Ala Arg Met Leu Asp Glu

485 490 495

Lys Pro Gly Ala Ile Ala Ala Thr Asn Phe Val Thr Gly Gln Gln Trp

500 505 510

Asp Gln Pro Asn Val Trp Pro Pro Leu Gln His Ile Leu Met Gln Gly

515 520 525

Leu Ile Asn Thr Pro Ala Thr Phe Gly Glu Asp Asp Pro Ala Tyr Gln

530 535 540

Ala Val Gln Asp Leu Ser Leu Arg Leu Ala Gln Arg Tyr Leu Asp Ser

545 550 555 560

Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu Thr Pro

565 570 575

Gln Leu Gln Gly Val Ser Pro Asp Ala Thr Gly Thr Met Phe Glu Lys

580 585 590

Tyr Ser Asp Ala Ala Ile Asn Val Ala Gly Gly Gly Gly Glu Tyr Glu

595 600 605

Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Ile Trp Ala Ala

610 615 620

Asp Thr Phe Ala Gly Arg Leu Thr Arg Pro Asp Cys Gly Asp Ile Glu

625 630 635 640

Ala Ala Gln Thr His Ser Gly Glu Arg Arg Ala Val Glu Leu Asp Pro

645 650 655

Arg Asp Ala Ala Trp Thr Lys Lys Phe Gly Lys Arg Ala Leu Lys Lys

660 665 670

Arg Ala Glu Ser Glu Arg Lys Lys Arg Val

675 680

<210> 80

<211> 692

<212> PRT

<213> 粗糙脉孢菌(Neurospora crassa)

<400> 80

Met Val Ser Arg Phe Leu Gly Ala Thr Val Pro Leu Ala Ala Ala Ile

1 5 10 15

Leu Pro Gly Ala Arg Ala Leu Tyr Val Asn Gly Ser Val Thr Ala Pro

20 25 30

Cys Asp Ser Pro Ile Tyr Cys Tyr Gly Glu Leu Leu His Gln Val Glu

35 40 45

Leu Ala Arg Pro Phe Ser Asp Ser Lys Thr Phe Val Asp Met Pro Thr

50 55 60

Ile Lys Pro Val Asp Glu Val Leu Glu Ala Phe Ser Lys Leu Thr Leu

65 70 75 80

Pro Leu Ser Asn Asn Ser Glu Leu His Glu Phe Leu Ser Thr Tyr Phe

85 90 95

Gly Pro Ala Gly Gly Glu Leu Glu Ala Val Pro Thr Asp Gln Leu His

100 105 110

Val Ser Pro Thr Phe Leu Asp Asn Val Ser Asp Asp Val Ile Lys Gln

115 120 125

Phe Val Asp Ser Val Ile Asn Ile Trp Pro Asp Leu Thr Arg Lys Tyr

130 135 140

Val Gly Ala Gly Glu Leu Cys Thr Gly Cys Ala Asp Ser Phe Ile Pro

145 150 155 160

Val Asn Arg Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr

165 170 175

Tyr Trp Asp Ser Phe Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly

180 185 190

Ala Phe Thr Glu Ile Ser Lys Asn Ile Ile Glu Asn Phe Leu Asp Leu

195 200 205

Val Glu Gln Ile Gly Phe Val Pro Asn Gly Ala Arg Leu Tyr Tyr Leu

210 215 220

Asp Arg Ser Gln Pro Pro Leu Leu Thr Gln Met Val Arg Ile Tyr Val

225 230 235 240

Glu His Thr Asn Asp Thr Ser Ile Leu Glu Arg Ala Val Pro Val Leu

245 250 255

Lys Lys Glu Trp Glu Trp Trp Thr Thr Asn Arg Thr Val Glu Val Thr

260 265 270

Ala Asp Gly Lys Thr Tyr Ser Leu Gln Arg Tyr His Val Asp Asn Asn

275 280 285

Gln Pro Arg Pro Glu Ser Tyr Arg Glu Asp Tyr Ile Thr Ala Asn Asn

290 295 300

Asn Ser Tyr Tyr Ala Thr Ser Gly Ile Ile Tyr Pro Glu Thr Thr Pro

305 310 315 320

Leu Asn Asp Thr Gln Lys Ala Leu Leu Tyr Ala Asn Leu Ala Ser Gly

325 330 335

Ala Glu Ser Gly Trp Asp Tyr Ser Ser Arg Trp Leu Lys Asn Pro Gly

340 345 350

Asp Ala Ala Arg Asp Val Tyr Phe Pro Leu Arg Ser Leu Asn Val Leu

355 360 365

Glu Ile Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Gln Asn Glu Val

370 375 380

Thr Ile Gly Lys Phe Leu Ala Gln Gln Gly Ser Lys Asp Glu Ala Glu

385 390 395 400

Glu Trp Ala Lys Lys Ala Glu Glu Arg Ser Glu Ala Met Tyr Lys Leu

405 410 415

Met Trp Asn Ser Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Leu Thr Ser

420 425 430

Ser Ser Gln Asn Ile Tyr Val Pro Ala Asp Pro Gln Val Phe Pro Phe

435 440 445

Glu Gln Pro Ser Gly Thr Pro Glu Gly Tyr Gln Val Leu Phe Ser Val

450 455 460

Asn Gln Met Phe Pro Phe Trp Thr Gly Ala Ala Pro Asp Gln Leu Lys

465 470 475 480

Gly Asn Pro Leu Ala Val Lys Leu Ala Phe Glu Arg Ile Lys Asn Leu

485 490 495

Leu Asp Asn Lys Ala Gly Gly Ile Pro Ala Thr Asn Phe Val Thr Gly

500 505 510

Gln Gln Trp Asp Glu Pro Asn Val Trp Pro Pro Leu Met His Val Leu

515 520 525

Met Asp Gly Leu Leu Asn Thr Pro Ala Thr Phe Gly Glu Asp Asp Pro

530 535 540

Ala Tyr Gln Glu Thr Gln Thr Leu Ala Leu Arg Leu Ala Gln Arg Tyr

545 550 555 560

Val Asp Ser Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser

565 570 575

Glu Thr Pro Lys Leu Gln Gly Leu Gly Ser Asp Leu Lys Gly Ile Met

580 585 590

Phe Glu Lys Tyr Ser Asp Asn Ser Thr Asn Val Ala Gly Ser Gly Gly

595 600 605

Glu Tyr Glu Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Ile

610 615 620

Trp Ala Ala Asp Lys Phe Gly Asp Lys Leu Lys Arg Pro Asp Cys Gly

625 630 635 640

Asp Ile Thr Pro Ala Gln Val Gly Lys Arg Ala Asp Ile Thr Met Glu

645 650 655

Lys Arg Ala Val Glu Leu Asp Val Phe Asp Ala Lys Phe Thr Lys Lys

660 665 670

Phe Ala Arg Lys Gly Lys Leu Glu Lys Leu Lys Ala Lys Phe Lys Arg

675 680 685

Arg Ala Ala Ile

690

<210> 81

<211> 682

<212> PRT

<213> 大丽轮枝菌(Verticillium dahliae)

<400> 81

Met Thr Leu Leu Ser Lys Ser Val Val Ala Leu Ala Ala Ala Ala Pro

1 5 10 15

Leu Ala Arg Ala Gln Leu Phe Arg Asn Gly Ser Val Ile Ala Pro Cys

20 25 30

Asp Ser Ala Ile Tyr Cys His Gly Glu Ile Leu Ser Gln Ile Gln Leu

35 40 45

Ala Arg Pro Phe Glu Asp Ser Lys Thr Phe Val Asp Met Pro Ala Lys

50 55 60

Arg Ser Leu Glu Gln Ile Gln Glu Ala Phe Asp Glu Leu Glu Lys Pro

65 70 75 80

Leu Thr Asn Asn Thr Ala Leu Asn Asp Phe Leu Ala Ala Asn Phe Ala

85 90 95

Leu Ala Gly Gly Glu Leu Ser Glu Val Asp Asp Leu Glu Thr Asp Ala

100 105 110

Gln Phe Leu Asp Asn Ile Asn Asp Thr Val Ile Arg Glu Phe Thr Glu

115 120 125

Lys Val Ile Glu Ile Trp Pro Asp Leu Thr Arg Ser Tyr Thr Gly Ala

130 135 140

Gly Ser Asn Cys Thr Asp Cys Pro Asn Ser Phe Ile Pro Ile Asn Arg

145 150 155 160

Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp

165 170 175

Ser Phe Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ser Phe Ile

180 185 190

Glu Val Ser Arg Asn His Ile Glu Asn Phe Leu Asp Leu Val Asp Gln

195 200 205

Tyr Gly Phe Val Met Asn Gly Ala Arg Arg Tyr Tyr Leu Asn Arg Ser

210 215 220

Gln Pro Pro Leu Leu Ser Gln Met Val Arg Leu Tyr Val Asp His Thr

225 230 235 240

Asn Asp Thr Asp Ile Leu Asp Arg Ala Leu Pro Leu Leu Ile Lys Glu

245 250 255

His Glu Trp Trp Thr Val Asn Arg Thr Val Glu Val Ser Lys Asp Gly

260 265 270

Arg Thr Tyr Gln Leu Asn Leu Tyr Asn Val Ser Asn Thr Gln Pro Arg

275 280 285

Pro Glu Ser Tyr Tyr Glu Asp Tyr Thr Thr Ala Ser Asn Ser Ser Tyr

290 295 300

Tyr Ala Glu Ser Gly Ile Ile Tyr Pro Glu Thr Arg Glu Leu Asn Asp

305 310 315 320

Thr Glu Lys Ala Phe Leu Tyr Ala Asn Leu Ala Ser Gly Ala Glu Ser

325 330 335

Gly Trp Asp Tyr Thr Ser Arg Trp Leu Ser Lys Pro Asn Asp Ala Val

340 345 350

Arg His Val Tyr Phe Pro Leu Arg Ser Leu Asn Ile Val Asn Ile Val

355 360 365

Pro Val Glu Leu Asn Ser Ile Leu Tyr Trp Asn Glu Val Thr Ile Ala

370 375 380

Gly Tyr Leu Asn Gln Thr Ser Asn Glu Thr Gln Ala Ala Glu Trp Thr

385 390 395 400

Glu Leu Ala Ala Asn Arg Ser Glu Ala Met Tyr Asn Leu Met Trp Asn

405 410 415

Asp Thr Leu Ser Gly Tyr Phe Asp Tyr Asn Leu Thr Ser Gly Ala Gln

420 425 430

Asp Thr Phe Val Pro Ala Asp Asp Asp Thr Thr Asp Leu Glu Ala Thr

435 440 445

Gly Ala Pro Glu Gly Gln Gln Val Tyr Phe Ser Val Ala Gln Phe Tyr

450 455 460

Pro Phe Trp Thr Gly Ala Ala Pro Ala His Ile Lys Asn Asn Pro Leu

465 470 475 480

Ala Val Lys Thr Ala Phe Glu Arg Val Ala Thr Tyr Leu Asp Thr Lys

485 490 495

Asp Gly Gly Ile Pro Ala Thr Asn Leu Arg Thr Gly Glu Gln Trp Asp

500 505 510

Gln Pro Ser Val Trp Pro Pro Leu Met His Ile Leu Met Glu Ala Leu

515 520 525

Arg Asn Thr Pro Ala Thr Phe Gly Glu Ser Asp Pro His Tyr Met Glu

530 535 540

Val Gln Asp Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp Ser Thr

545 550 555 560

Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Asp Thr Pro Gln

565 570 575

Leu Glu Gly Phe Asp Glu Arg Glu Val Gly Ile Met Phe Glu Lys Tyr

580 585 590

Ser Asp Asn Ser Thr Asn Gln Ala Gly Gly Gly Gly Glu Tyr Glu Val

595 600 605

Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Leu Trp Val Val Asp

610 615 620

Thr Phe Gly Asn Asn Leu Thr Arg Pro Asp Cys Gly Asp Ile Glu Ala

625 630 635 640

Ala Asn Val Gln Pro Ser Lys Arg Ser Ala Val Glu Leu His Arg Arg

645 650 655

Asp Ala Ala Trp Thr Lys Lys Phe Gly Ser Arg Ser Ala Lys Gly Glu

660 665 670

Ala Lys Arg Phe Ala Lys Leu Arg Gly Tyr

675 680

<210> 82

<211> 688

<212> PRT

<213> 玉蜀黍赤霉(Gibberella zeae)

<400> 82

Met Pro Ser Pro Arg His Ile Ala Ala Ala Leu Val Ala Thr Ala Thr

1 5 10 15

Thr Ala Ser Ala Leu Tyr Val Asn Gly Thr Val Val Ala Pro Cys Asp

20 25 30

Ser Pro Ile Tyr Cys His Gly Asp Ile Leu Glu Gln Val Glu Leu Ala

35 40 45

Arg Pro Phe Ser Asp Ser Lys Thr Phe Val Asp Met Pro Ala Ile Arg

50 55 60

Pro Leu Ser Asp Ile Gln Glu Ala Phe Asp Lys Leu Glu Lys Pro Leu

65 70 75 80

Arg Asn Asn Ser Ala Leu Ala Asp Phe Leu Asp Glu Asn Phe Asp Asp

85 90 95

Ala Gly Asn Glu Leu Glu Glu Val Ser Arg Asp Asp Leu Asp Thr Asp

100 105 110

Pro Lys Phe Leu Asp Asn Ile Asn Asp Thr Val Ile Arg Glu Phe Thr

115 120 125

Glu Lys Val Ile Asp Ile Trp Pro Asp Leu Thr Arg Arg Tyr Asp Gln

130 135 140

Asp Ala Lys Asn Cys Ser Asp Cys Pro Asn Ser Phe Ile Pro Val Asn

145 150 155 160

Arg Ser Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp

165 170 175

Asp Ser Tyr Trp Ile Ile Leu Gly Leu Leu Arg Thr Gly Gly Ser Phe

180 185 190

Ile Glu Ile Ala Lys Asn Thr Ile Glu Asn Phe Leu Asp Phe Ile Glu

195 200 205

Glu Tyr Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Leu Asn Arg

210 215 220

Ser Gln Pro Pro Leu Leu Ser Gln Met Ile Lys Ala Tyr Val Glu Tyr

225 230 235 240

Thr Asn Asp Thr Asp Ile Leu Glu Arg Ala Leu Pro Leu Leu Val Gln

245 250 255

Glu His Glu Phe Phe Met Asn Asn Arg Ser Val Pro Val Tyr Ile Asn

260 265 270

Asn Glu Thr Tyr Tyr Leu Asn Thr Tyr Asn Val Ser Asn Thr Arg Pro

275 280 285

Arg Pro Glu Ser Tyr Arg Glu Asp Tyr Ile Thr Ala Glu Asn Thr Ser

290 295 300

Tyr Tyr Ser Pro Glu Ser Gly Lys Val Tyr Lys Gly Gly Glu Glu Leu

305 310 315 320

Ser Phe Lys Gln Lys Glu Ala Leu Tyr Gly Asn Leu Ala Ser Gly Ala

325 330 335

Glu Ser Gly Leu Asp Tyr Thr Val Lys Trp Ile Ala Arg Pro Glu Asp

340 345 350

Ala Ile Arg Asp Asn Tyr Phe Pro Leu Arg Tyr Leu Asn Thr Arg Asn

355 360 365

Ile Ile Pro Val Asp Leu Asn Ser Ile Leu Tyr Gly Asn Glu Ile Ala

370 375 380

Ile Ala Asp Phe Tyr Glu Gln Thr Gly Asn Asn Ser Ala Ser Glu Gln

385 390 395 400

Trp Arg Glu Val Ala Ala Asn Arg Ser Tyr Ala Met His Gly Phe Leu

405 410 415

Trp Asn Glu Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Leu Thr Ser Lys

420 425 430

Ala Gln Gln Ile Tyr Phe Pro Val Asp Glu Asn Thr Thr Val Val Asp

435 440 445

Thr Glu Asp Ala Pro Lys Gly Gln Gln Val Phe Phe Ser Pro Thr Gln

450 455 460

Phe Tyr Pro Phe Trp Leu Gly Ala Ala Pro Asp Tyr Leu Lys Asn Asn

465 470 475 480

Pro Phe Ala Val Tyr Thr Ala Tyr Lys Arg Val Glu Tyr Tyr Leu Asp

485 490 495

Asn Arg Asp Gly Gly Ile Pro Ala Ser Asn Val Glu Thr Gly Gln Gln

500 505 510

Trp Asp Gln Pro Asn Val Trp Pro Pro Leu Met His Ile Leu Met Ala

515 520 525

Gly Leu Glu Arg Val Pro Pro Thr Phe Gly Ile Arg Asp Pro Ser Phe

530 535 540

Val Glu Val Arg Arg Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp

545 550 555 560

Ser Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu Thr

565 570 575

Pro Lys Leu Gln Ser Thr Ser Asp Glu Glu Glu Gly Ile Met Phe Glu

580 585 590

Lys Tyr Ala Asp Asn Ala Thr Asn Val Ala Gly Gly Gly Gly Glu Tyr

595 600 605

Glu Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Ile Trp Ala

610 615 620

Val Asp Glu Phe Lys Asn Arg Leu Thr Arg Pro Lys Cys Asp Asn Leu

625 630 635 640

Glu Ser Ala His Ser Asn Asp Lys Arg Asp Pro Ser Ala Val Met Leu

645 650 655

Asn Ala Arg Asp Ala Lys Arg Val Lys Lys Phe Gly Arg Arg Lys Arg

660 665 670

Ala Ala Glu Lys Ala Ala Lys Lys Arg Ser Ser Arg Val Phe Tyr Phe

675 680 685

<210> 83

<211> 15

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 83

Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn

1 5 10 15

<210> 84

<211> 17

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 84

Gly Val Ala Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp

1 5 10 15

Asp

<210> 85

<211> 20

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 85

Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Glu Ile Lys

1 5 10 15

Gln Ala Asp Val

20

<210> 86

<211> 13

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 86

Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val

1 5 10

<210> 87

<211> 698

<212> PRT

<213> 稻瘟病菌(Magnaporthe grisea)

<400> 87

Met Ser Pro Leu Trp Lys Thr Ala Ala Ala Ile Ala Val Ala Ala Ser

1 5 10 15

Gly Ser Leu Val Asn Ala Val Tyr Ile Asn Gly Ser Ile Ile Thr Pro

20 25 30

Cys Asp Ser Leu Ile Tyr Cys Arg Gly Glu Leu Leu Lys Glu Val Glu

35 40 45

Leu Ala His Pro Phe Ala Asp Ser Lys Thr Phe Val Asp Met Pro Thr

50 55 60

Ile Lys Pro Val Asp Glu Val Ile Glu Ala Phe Asn Lys Leu Gln Lys

65 70 75 80

Pro Leu Ser Asn Asn Thr Glu Leu Gln Asp Phe Leu Arg Glu Asn Phe

85 90 95

Ala Gln Ala Gly Gly Glu Leu Glu Glu Val Pro Asn Ser Glu Leu Glu

100 105 110

Thr Asp Pro Val Phe Leu Asp Lys Leu Asp Asp Thr Val Ile Arg Glu

115 120 125

Phe Val Glu Lys Val Ile Asp Ile Trp Pro Ser Leu Thr Arg Arg Tyr

130 135 140

Lys Gly Pro Ser Asn Cys Glu Ala Cys Ala Asp Ser Phe Ile Pro Val

145 150 155 160

Asn Arg Thr Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr

165 170 175

Trp Asp Ser Tyr Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala

180 185 190

Phe Thr Asn Ile Ser Lys Asn Thr Val Glu Asn Phe Leu Asp Leu Val

195 200 205

Glu Thr Ile Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Lys Asn

210 215 220

Arg Ser Gln Pro Pro Leu Leu Ser Gln Met Val Arg Ile Tyr Val Glu

225 230 235 240

His Thr Asn Asp Thr Ser Ile Leu Gly Arg Ala Val Pro Leu Leu Ile

245 250 255

Lys Glu His Glu Phe Phe Ile Asn Asn Arg Ser Ile Asp Val Glu Ala

260 265 270

Ser Asn Gly Lys Thr Tyr Arg Leu Gln Arg Tyr Ala Val Thr Asn Thr

275 280 285

Gln Pro Arg Pro Glu Ser Tyr Arg Glu Asp Tyr Ile Thr Ala Ser Asn

290 295 300

Arg Ser Tyr Tyr Ser Pro Ser Gly Ile Ile Tyr Pro Glu Ser His Gln

305 310 315 320

Leu Asn Glu Ser Glu Lys Ala Val Leu Tyr Ser His Leu Ala Ser Gly

325 330 335

Ala Glu Ser Gly Trp Asp Tyr Thr Ser Arg Trp Leu Ser Thr Pro Ser

340 345 350

Asp Ala Val Arg Asp Asn Tyr Phe Pro Leu Arg Ser Leu Asn Thr Asn

355 360 365

Asn Ile Val Pro Val Asp Leu Asn Ser Ile Leu Tyr Ala Asn Glu Val

370 375 380

Ala Ile Ala Glu Phe Leu Asn Arg Thr Gly Asn Ser Thr Gly Ala Ser

385 390 395 400

Asp Trp Met Asp Leu Ala Lys Gln Arg Ser Glu Ala Met Tyr Ala Leu

405 410 415

Met Trp Asn Glu Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Met Thr Ser

420 425 430

Lys Thr Gln Asn Arg Phe Ile Pro Val Asp Glu Asp Ala Val Ser Ile

435 440 445

Glu Thr Asn Asn Ala Pro Ala Gly Gln Gln Val Phe Phe His Val Ala

450 455 460

Gln Tyr Tyr Pro Phe Trp Thr Gly Ala Ala Pro Arg Ser Leu Lys Asn

465 470 475 480

Asn Pro Leu Ala Val Leu Arg Ala Tyr Glu Arg Ile Asp Ala Tyr Leu

485 490 495

Asp Ile Lys Arg Gly Ala Ile Pro Ala Thr Asn Leu Lys Thr Gly Gln

500 505 510

Gln Trp Asp Glu Pro Asn Val Trp Pro Pro Leu Met His Ile Leu Met

515 520 525

Glu Gly Leu Thr Arg Val Pro Ala Thr Phe Gly Glu Asp Asp Val Ala

530 535 540

Trp Thr Glu Ile Gln Asp Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu

545 550 555 560

Asp Ser Thr Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu

565 570 575

Thr Pro Gln Leu Gln Gly Leu Asn Ala Glu Asp Lys Gly Ile Met Phe

580 585 590

Glu Lys Tyr Gly Asp Asn Ser Thr Asn Val Ala Gly Ser Gly Gly Glu

595 600 605

Tyr Glu Val Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Met Trp

610 615 620

Val Ala Asp Thr Phe Asn Asn Lys Leu Thr Arg Pro Asp Cys Gly Asn

625 630 635 640

Ile Thr Ala Ala Asn Val His Ser Asp Gly Ser Gln Ala Arg Lys Arg

645 650 655

Gly Glu Met Trp Ser Ala Leu Glu Met His Pro Tyr Asp Ala Ala Trp

660 665 670

Thr Lys Glu Phe Gly Ala Arg Lys Val Arg Arg Asp Lys Ala Glu Ala

675 680 685

Arg Ala Leu Gly Asn Val Met Gly Gly Val

690 695

<210> 88

<211> 676

<212> PRT

<213> 稻瘟病菌(Magnaporthe grisea)

<400> 88

Val Tyr Ile Asn Gly Ser Ile Ile Thr Pro Cys Asp Ser Leu Ile Tyr

1 5 10 15

Cys Arg Gly Glu Leu Leu Lys Glu Val Glu Leu Ala His Pro Phe Ala

20 25 30

Asp Ser Lys Thr Phe Val Asp Met Pro Thr Ile Lys Pro Val Asp Glu

35 40 45

Val Ile Glu Ala Phe Asn Lys Leu Gln Lys Pro Leu Ser Asn Asn Thr

50 55 60

Glu Leu Gln Asp Phe Leu Arg Glu Asn Phe Ala Gln Ala Gly Gly Glu

65 70 75 80

Leu Glu Glu Val Pro Asn Ser Glu Leu Glu Thr Asp Pro Val Phe Leu

85 90 95

Asp Lys Leu Asp Asp Thr Val Ile Arg Glu Phe Val Glu Lys Val Ile

100 105 110

Asp Ile Trp Pro Ser Leu Thr Arg Arg Tyr Lys Gly Pro Ser Asn Cys

115 120 125

Glu Ala Cys Ala Asp Ser Phe Ile Pro Val Asn Arg Thr Phe Val Val

130 135 140

Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp Ser Tyr Trp Ile

145 150 155 160

Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe Thr Asn Ile Ser Lys

165 170 175

Asn Thr Val Glu Asn Phe Leu Asp Leu Val Glu Thr Ile Gly Phe Val

180 185 190

Pro Asn Gly Ala Arg Ile Tyr Tyr Lys Asn Arg Ser Gln Pro Pro Leu

195 200 205

Leu Ser Gln Met Val Arg Ile Tyr Val Glu His Thr Asn Asp Thr Ser

210 215 220

Ile Leu Gly Arg Ala Val Pro Leu Leu Ile Lys Glu His Glu Phe Phe

225 230 235 240

Ile Asn Asn Arg Ser Ile Asp Val Glu Ala Ser Asn Gly Lys Thr Tyr

245 250 255

Arg Leu Gln Arg Tyr Ala Val Thr Asn Thr Gln Pro Arg Pro Glu Ser

260 265 270

Tyr Arg Glu Asp Tyr Ile Thr Ala Ser Asn Arg Ser Tyr Tyr Ser Pro

275 280 285

Ser Gly Ile Ile Tyr Pro Glu Ser His Gln Leu Asn Glu Ser Glu Lys

290 295 300

Ala Val Leu Tyr Ser His Leu Ala Ser Gly Ala Glu Ser Gly Trp Asp

305 310 315 320

Tyr Thr Ser Arg Trp Leu Ser Thr Pro Ser Asp Ala Val Arg Asp Asn

325 330 335

Tyr Phe Pro Leu Arg Ser Leu Asn Thr Asn Asn Ile Val Pro Val Asp

340 345 350

Leu Asn Ser Ile Leu Tyr Ala Asn Glu Val Ala Ile Ala Glu Phe Leu

355 360 365

Asn Arg Thr Gly Asn Ser Thr Gly Ala Ser Asp Trp Met Asp Leu Ala

370 375 380

Lys Gln Arg Ser Glu Ala Met Tyr Ala Leu Met Trp Asn Glu Thr Leu

385 390 395 400

Trp Ser Tyr Phe Asp Tyr Asn Met Thr Ser Lys Thr Gln Asn Arg Phe

405 410 415

Ile Pro Val Asp Glu Asp Ala Val Ser Ile Glu Thr Asn Asn Ala Pro

420 425 430

Ala Gly Gln Gln Val Phe Phe His Val Ala Gln Tyr Tyr Pro Phe Trp

435 440 445

Thr Gly Ala Ala Pro Arg Ser Leu Lys Asn Asn Pro Leu Ala Val Leu

450 455 460

Arg Ala Tyr Glu Arg Ile Asp Ala Tyr Leu Asp Ile Lys Arg Gly Ala

465 470 475 480

Ile Pro Ala Thr Asn Leu Lys Thr Gly Gln Gln Trp Asp Glu Pro Asn

485 490 495

Val Trp Pro Pro Leu Met His Ile Leu Met Glu Gly Leu Thr Arg Val

500 505 510

Pro Ala Thr Phe Gly Glu Asp Asp Val Ala Trp Thr Glu Ile Gln Asp

515 520 525

Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp Ser Thr Phe Cys Thr

530 535 540

Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu Thr Pro Gln Leu Gln Gly

545 550 555 560

Leu Asn Ala Glu Asp Lys Gly Ile Met Phe Glu Lys Tyr Gly Asp Asn

565 570 575

Ser Thr Asn Val Ala Gly Ser Gly Gly Glu Tyr Glu Val Val Glu Gly

580 585 590

Phe Gly Trp Thr Asn Gly Val Leu Met Trp Val Ala Asp Thr Phe Asn

595 600 605

Asn Lys Leu Thr Arg Pro Asp Cys Gly Asn Ile Thr Ala Ala Asn Val

610 615 620

His Ser Asp Gly Ser Gln Ala Arg Lys Arg Gly Glu Met Trp Ser Ala

625 630 635 640

Leu Glu Met His Pro Tyr Asp Ala Ala Trp Thr Lys Glu Phe Gly Ala

645 650 655

Arg Lys Val Arg Arg Asp Lys Ala Glu Ala Arg Ala Leu Gly Asn Val

660 665 670

Met Gly Gly Val

675

<210> 89

<211> 1109

<212> PRT

<213> 光滑假丝酵母(Candida glabrata)

<400> 89

Ser Thr Asn Ser Tyr Phe Ser Ser Ser Leu Ala Ser Ala Ser Pro Leu

1 5 10 15

Phe Asn Lys Glu Gly Arg Val Val Arg Pro Met Arg Glu Ser Asn Leu

20 25 30

Gly Leu His Ala Asp Pro Gln Thr Arg Lys Ser Ser Lys Thr Leu Tyr

35 40 45

Asp Leu Leu Ser Asp Phe Asp Asn Ala Phe Tyr Asp Asp Glu Asn Met

50 55 60

Ile Leu Gly Ser Leu Ala Phe Gly Glu Asn Thr Tyr Ser Arg Gln Pro

65 70 75 80

Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn Ile Gly Phe

85 90 95

Gly Tyr Ala Leu Asp Thr Leu Asn Leu Tyr Ala Asp Ala Pro Gly Ala

100 105 110

Leu Asn Asn Gly Trp Pro Leu Arg Asn Arg Arg Phe Ala Gly Ser Phe

115 120 125

Val Ser Asp Phe Tyr Ser Leu Gln Ala Lys Leu Asn Ser Thr Asn Phe

130 135 140

Pro Glu Leu Asp Glu Lys Gly Tyr Thr Thr Val Ile Ser Ser Ile Pro

145 150 155 160

Glu Trp Thr Asp Leu Gln Phe Thr Val Asp Leu Asn Gly Thr Asn Trp

165 170 175

Phe Asn Pro Gln Ser Val Leu Ile Asp Asp Val Ile Asn Tyr Asn Gln

180 185 190

Asn Leu Ser Met Lys Asp Gly Ile Val Ser Thr Asn Met Asp Trp Leu

195 200 205

Asn Gly Met Ile Asn Ile Lys Ser Glu Val Trp Ala His Arg Lys Ile

210 215 220

His Ser Leu Gly Ile Thr Arg Leu Glu Ile Ser Leu Asn Leu Asp Ala

225 230 235 240

Leu Pro Asp Glu Phe Thr Glu Leu Pro Val Thr Ile Tyr Asp Ile Ile

245 250 255

Asp Phe Asn Thr Ser His Arg Thr Thr Leu Tyr Glu Lys Gly Gln Asp

260 265 270

Glu Asp Asn Lys Ala Ile Tyr Met Ile Val Asn Pro Glu Asn Val Pro

275 280 285

Tyr Ser Asn Ala Val Val Tyr Ser Thr Cys Thr Ile Lys Gly Thr Glu

290 295 300

Asn Asn Phe Ser Pro Tyr Asn Phe Thr Ser Asp Asp Arg Ile Ala Arg

305 310 315 320

Asn Tyr Met Thr Asn Leu Thr Glu Glu Asn Pro Lys Val Val Ile Tyr

325 330 335

Lys Tyr Thr Ser Val Val Ser Ser Glu Tyr Asn Asn Asp Glu Pro Asn

340 345 350

Pro Asn Val Asn Leu Lys Phe Ala Ser Asn Ile Ala Asn Thr Ala Lys

355 360 365

Gly Asn Tyr Lys Ser Leu Leu Ser Asn His Lys Arg Ala Trp Tyr Asp

370 375 380

Leu Tyr Asn Asp Ala Phe Ile Glu Ile Pro Ser Asp Ser Leu Leu Glu

385 390 395 400

Met Thr Ala Arg Ser Ser Leu Phe His Leu Leu Ala Asn Thr Arg Gln

405 410 415

Tyr Asn Val Ser Thr Thr Arg Gly Leu Pro Val Gly Val Gly Gly Leu

420 425 430

Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Ala Asp Val Trp

435 440 445

Met Ala Pro Ala Leu Leu Pro Phe Phe Pro Asn Ile Ala Met Asn Met

450 455 460

Asn Asn Tyr Arg Asn Ala Thr His Gln Gln Ala Ile Glu Asn Ala Lys

465 470 475 480

Gln Tyr Asn Tyr Pro Gly Ala Val Tyr Pro Trp Thr Ser Gly Arg Tyr

485 490 495

Ala Asn Cys Thr Ser Thr Gly Pro Cys Ile Asp Tyr Glu Tyr His Ile

500 505 510

Asn Val Asp Ile Ala Leu Ala Ser Phe Ser Ile Tyr Met Asn Gly Ala

515 520 525

Glu Gly Ala Asp Glu Asp Tyr Leu Arg Phe Thr Thr Trp Pro Met Val

530 535 540

Lys Asp Ala Ala Val Phe Phe Lys Ala Tyr Val Lys Tyr Asn Glu Thr

545 550 555 560

Leu Gly Glu Tyr Glu Thr Tyr Asn Leu Thr Asp Pro Asp Glu Phe Ala

565 570 575

Asn His Val Asn Asn Gly Ala Phe Thr Asn Ala Gly Ile Lys Thr Leu

580 585 590

Leu Lys Trp Ala Thr Asp Ile Gly Thr His Leu Gly Glu Glu Val Asp

595 600 605

Pro Lys Trp Met Glu Ile Ala Asp Asn Ile His Ile Pro Arg Ser Asp

610 615 620

Ser Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser Ser Val Glu Ile

625 630 635 640

Lys Gln Ala Asp Val Thr Leu Met Val Tyr Pro Leu Gly Tyr Ile Asn

645 650 655

Asp Glu Ser Ile Leu Asn Asn Ala Ile Lys Asp Leu Tyr Tyr Tyr Ser

660 665 670

Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr Tyr Pro Val Phe Val

675 680 685

Ala Ala Ala Ala Ser Leu Leu Asn His Gly Ser Ser Ser Gln Ser Tyr

690 695 700

Leu Tyr Lys Ser Val Leu Pro Tyr Leu Arg Ser Pro Phe Ala Gln Phe

705 710 715 720

Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly Leu Thr Gln Pro

725 730 735

Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Phe Leu Gln Ser Ile Leu

740 745 750

Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr Glu Val Thr Pro Arg Thr

755 760 765

Lys Lys Ile Ser Arg Leu Leu Lys Phe Asp Pro Val Lys Leu Pro Leu

770 775 780

Leu Pro Gly Gly Ile Ala Ile Arg Asn Phe Lys Tyr Met Gly Gln Val

785 790 795 800

Leu Asp Ile Ile Ile Asp Asp Asn Asn Gly Thr Ile Ala His Lys Gly

805 810 815

Gly Asp Lys Pro Ile Arg Ile Lys Val Pro Asn Arg Asp Ile Leu His

820 825 830

Asp Arg Asn Ile Thr Ser Ala Leu Tyr Ser Lys Arg Asp Asp Asp Leu

835 840 845

Ser Ala Thr Asp Asp Tyr Tyr Gly Thr Tyr Phe Thr Leu Tyr Pro Asn

850 855 860

Glu Glu Leu Val Ile Pro Leu Tyr Asp Thr Lys Leu Asn Ile Asp Gly

865 870 875 880

Asn Ile Ala Glu Ser Lys Gln Ile Thr Asn Leu Thr Ala Gly Val Pro

885 890 895

Gly Asp Val Gly Phe Ser Ala Leu Asp Gly Asn Asn Tyr Thr His Trp

900 905 910

Gln Pro Phe Asp Lys Ser Asp Asn Ala Lys Leu Leu Ile Asp Leu Gly

915 920 925

Phe Asn Ser Thr His Val Ile Lys Lys Gly Ile Ile Leu Trp Gly Gln

930 935 940

Arg Pro Ala Lys Asn Ile Ser Leu Ser Val Leu Pro His Ser Glu Arg

945 950 955 960

Ile Glu Gln Leu Phe Ala Asn Ile Thr Asp Leu Leu Glu Thr Ser Ser

965 970 975

Ile Thr Lys Gly Gly Ser Leu Leu Asn Gln Met Leu Gly Gln Thr Gln

980 985 990

Ser Asn Val Thr Ala Glu Ile Asp Asp Asp Ile Leu Ala Leu Leu Asn

995 1000 1005

Trp Lys Gly Asp Asp Leu Asp Gln Leu Ile Pro Tyr Leu Pro Asp

1010 1015 1020

Met His Leu Leu Gln Glu Lys Phe Ile Pro Ile Leu Lys Asp Tyr

1025 1030 1035

Pro Ile Lys Pro Asn Gln Arg Tyr Tyr Lys Glu Ile Ile Asp Asp

1040 1045 1050

Asp Ile Ile Lys Leu Leu Pro Ser Asn Thr Thr Glu Phe Thr Ile

1055 1060 1065

Asp Tyr Asn Ser Ile Pro Gly Gly Glu Lys Arg Ala Arg Tyr Val

1070 1075 1080

Val Leu Thr Val His Gly Thr Tyr Asp Asp Asp Asp Asp Leu Lys

1085 1090 1095

Gly Ala Thr Ile Lys Glu Ile Val Leu Gln Glu

1100 1105

<210> 90

<211> 1020

<212> PRT

<213> 近平滑假丝酵母(Candida parapsilosis)

<400> 90

Arg Phe Ser Glu Ala Phe Pro Phe Val Leu Pro Arg Ala Glu Lys Tyr

1 5 10 15

Thr Leu Glu Gln Thr Ser Asp Ser Asn Leu Gly Asp Phe Val Asn Ser

20 25 30

Phe Glu Asn Lys Glu Ile Phe Ser Gln Val Gln Phe Ser Glu His Ala

35 40 45

Phe Tyr Asp Arg Lys Met Asn Val Val Gly Thr Ile Glu Tyr Ser Lys

50 55 60

Tyr Asn Gln Phe Gln Lys Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly

65 70 75 80

Ser Arg Ile Pro Asn Leu Gly His Gly Phe Thr Tyr Asp Gln Leu Thr

85 90 95

Glu Ser Pro Asp Ser Lys Lys Asp Asp Leu Tyr Asn Gly Trp Pro Leu

100 105 110

Phe Asp Lys Arg Phe Ser Gly Ala Phe Ile Ala Gly Phe Tyr Asp Leu

115 120 125

Gln Glu Asn Thr Thr Gly Asn Asn Phe Pro Glu Leu Leu Lys Asn Gly

130 135 140

Tyr Glu Ser Val Ile Ser Ala Ile Pro Gln Trp Thr Thr Leu Lys Leu

145 150 155 160

Ala Ile Gln His Gly Asp Lys Arg Tyr Thr Leu Asp Pro Ala Ser Pro

165 170 175

Asp Gly Val Gly Gln Ile Thr Asn Tyr Val Gln Asn Met Ser Leu Ser

180 185 190

Thr Gly Ile Val Thr Thr Gln Phe Thr Trp Leu Glu Ser Leu Asp Val

195 200 205

Arg Tyr Thr Val Leu Ala His Arg Ser Glu Ile Asn Leu Gly Leu Val

210 215 220

Lys Val Asp Ile Lys Asn Lys Gly Asn Asp Thr Val Glu Ile Asp Val

225 230 235 240

Ile Asp Glu Leu Asn Phe Asp Thr Ala Gln Arg Cys Ser Leu Asn Leu

245 250 255

Ile Gly His Asp Asn Ser Gly Ile Tyr Val Ser Phe Ser Pro Asp Asn

260 265 270

Leu Asp Tyr Ile Ser Gly Ala Ile Tyr Ser Thr Leu His Leu Asp Ser

275 280 285

Val Lys Arg His Ser Lys Asn Lys Lys Thr Asn Gln Thr Ser Ser Ile

290 295 300

Arg Leu His Ala Gln Asp Ser Thr Gln Phe Gly Lys Thr Val Gly Ile

305 310 315 320

Val Ser Thr Asp Leu Asp Pro Ser Lys Phe Lys Asn Ser Arg Asp Thr

325 330 335

Leu Glu Phe Ala Lys Lys Val Ser Gln Lys Phe Glu Tyr Leu Asp Leu

340 345 350

Val Ile Lys Ser His Val Arg Ala Trp Lys Glu Leu Met Gly Lys Gln

355 360 365

Ser Thr Ile Thr Phe Pro Asn Asp Pro Leu Leu Asp Leu Gly Ser Lys

370 375 380

Ala Ser Val Tyr His Leu Leu Ala Asn Thr Arg Pro Asp Ala Gln Gly

385 390 395 400

Leu Thr Gly Ala Leu Gly Val Ala Gly Leu Ser Ser Asp Ser Tyr Gly

405 410 415

Gly Met Val Phe Trp Asp Thr Asp Phe Trp Met Phe Arg Ala Ile Leu

420 425 430

Ala Leu Asp Pro Thr His Ala Lys Ser Ile Val Asn Tyr Arg Met His

435 440 445

Thr His Gln Gln Ala Leu Lys Asn Thr Pro Gln Gly Tyr Glu Gly Ala

450 455 460

Ala Tyr Pro Trp Thr Ser Gly Arg Phe Gly Asn Cys Thr Ala Thr Gly

465 470 475 480

Pro Cys Leu Asn Tyr Glu Tyr His Ile Asn Ser Ala Ile Ala Met Ala

485 490 495

Ala Trp Glu Leu Tyr Ile Ser Gly Ala Val Asp Asp Gln Tyr Leu Glu

500 505 510

Ser Thr Val Tyr Pro Leu Ile Ser Asp Ala Ala Lys Phe Tyr Ala Asp

515 520 525

Tyr Val Thr Ser Tyr Asn Glu Thr Ile Asp Lys Phe Ile Thr Ser Asn

530 535 540

Leu Thr Asp Pro Asp Glu Tyr Ala Asn His Val Asp Asn Gly Ala Tyr

545 550 555 560

Thr Asn Ala Gly Ile Ala Leu Leu Met Asn Trp Ile Asn Asp Ile Gly

565 570 575

Asp Ile Leu Gly Ile Lys Met Pro Glu Ile Tyr Thr Asp Ile Ser Gln

580 585 590

Lys Met Tyr Leu Pro Thr Ala Asp Asn Ser Gln Asn Ile Thr Leu Glu

595 600 605

Tyr Ser Gly Met Asn Ser Ser Val Gly Ile Lys Gln Ala Asp Val Val

610 615 620

Leu Met Thr Tyr Pro Leu Gln Asn Ser Leu Val Asp Asp Glu Gln Ala

625 630 635 640

Tyr Ile Asn Met Glu Phe Tyr Ser Gly Lys Gln Val Ser Tyr Gly Pro

645 650 655

Ala Met Thr Phe Ser Ile Phe Ser Ile Val Ala Ala Asn Leu Ala Thr

660 665 670

Ser Gly Cys Ala Ser Gln Ser Tyr Leu His Lys Ala Met Gln Pro Tyr

675 680 685

Leu Arg Gly Pro Phe Ala Gln Phe Ser Glu Gln Asn Asn Asp Asn Phe

690 695 700

Lys Thr Asn Gly Gly Thr His Pro Ala Tyr Pro Phe Leu Thr Ala His

705 710 715 720

Gly Gly Phe Ile Gln Ala Val Val Asn Gly Leu Thr Gly Leu Arg His

725 730 735

Ser Tyr Thr Ile Asp Asn Gly Lys Ile Arg Arg Ser Leu Phe Leu Asp

740 745 750

Pro Ile Ala Leu Pro Cys Leu Gly Asp Gly Val Gln Tyr Ser Gly Ile

755 760 765

His Tyr Asp Asn His Thr Ile Ser Met Asn Ile Thr Gly Ser Thr Phe

770 775 780

Val Ile Arg Asn Glu Gly Lys Thr Asn Lys Arg Ala Asn Asp Tyr Ile

785 790 795 800

Ser Ile Thr Ile Ala Asp Arg Asn Pro Gly Arg Gly Thr Tyr Lys Leu

805 810 815

Lys Asp Asn Glu Glu Lys Ser Phe Lys Leu Tyr Gln Pro Ser Lys Ile

820 825 830

Phe Glu Asp Ser Val Ser Glu Cys Gly Leu Ala Thr Phe Tyr Asn Ile

835 840 845

Thr Glu Ser Ala Pro Gly Asp Ser Ser Phe Ser Ile Asn Asp Gly Asp

850 855 860

Asn Thr Thr Arg Trp Gln Val Lys Tyr Asn Asp Thr Thr Gly Lys Val

865 870 875 880

Leu Ile Asp Leu His Ser Ile Lys Asn Ile Thr Gly Val Val Phe Asn

885 890 895

Trp Ala Asp Lys Pro Pro Lys Lys Val Lys Leu Ser Lys Tyr Met Gly

900 905 910

Thr Lys Phe Lys Asp Val Thr Asp Phe Leu Ala Lys Val Asp Phe Gly

915 920 925

Asn Tyr Val Tyr Lys Asn Tyr Arg Phe Ala Asn Pro Gly Glu Lys Leu

930 935 940

Leu Asn Gln Ser Glu Val Phe Glu Asn Val Tyr Ser Asp Asp Val Thr

945 950 955 960

Ile Asn Ala Pro Phe Ser Ser Lys Glu Leu Glu Glu Val Ile Val Pro

965 970 975

Val Arg His Asn Thr Thr Ser Trp Asn Ile Asn Leu Gln Gly Arg Phe

980 985 990

Leu Leu Leu Glu Val Asp Gly Ile His Asn Thr Ile Pro Ile Glu Asp

995 1000 1005

Asp Tyr Gly Gly Ala Lys Leu Ala Glu Val Asn Phe

1010 1015 1020

<210> 91

<211> 1126

<212> PRT

<213> 乳酸克鲁维酵母(Kluyveromyces lactis)

<400> 91

Thr Lys Pro Leu Pro Leu Gln His Thr His Phe Gly Ser Phe Val Cys

1 5 10 15

Ala Glu Asp Thr Leu Phe Cys Pro Glu Ser Gln Arg Lys Ala Ser Glu

20 25 30

Lys Met Tyr Ser Leu Leu Lys Asp His Glu Asn Thr Phe Tyr Asp Glu

35 40 45

Glu Gln Gln Ile Leu Gly Asn Leu Leu Leu Ser Glu Asn Thr Phe Ser

50 55 60

Arg Gln Pro Tyr Val Ala Asn Gly Tyr Ile Gly Ser Arg Ile Pro Asn

65 70 75 80

Leu Gly Phe Gly Tyr Ala Leu Asp Thr Ile Asn Val Trp Val Asn Asp

85 90 95

Ser Ser Ile Pro Gly Ala Leu Asp Asn Gly Trp Pro Leu Arg Asn Gln

100 105 110

Arg Phe Ala Gly Ala Phe Ile Ser Asp Phe Tyr Cys Leu Gln Glu Lys

115 120 125

Leu Asn Ser Thr Asn Phe Pro Glu Leu Asp Asp Asp Gly Tyr Ser Thr

130 135 140

Val Ile Ser Thr Ile Pro Gln Trp Thr Asp Leu Ser Ile Leu Lys His

145 150 155 160

Thr Thr Thr Gly Gln Ile Glu Tyr Ile Asn Pro Thr Asp Val Lys Leu

165 170 175

Asp Lys Ile Thr Asn Tyr Met Gln Asn Leu Ser Leu Gln Asp Gly Ile

180 185 190

Val Thr Thr Thr Phe Val Tyr Asp Lys Gln Leu Leu Val Thr Thr Lys

195 200 205

Val Val Ala His Arg Lys Ile Tyr Pro Leu Gly Val Val Thr Leu Glu

210 215 220

Leu Ser Leu Phe Asp Asn Asn Ser Asp Thr Ser Ala Asp Asn Glu Asn

225 230 235 240

Glu Tyr Val Glu Leu Glu Ile Cys Asp Ser Leu Asn Phe Ser Thr Ser

245 250 255

His Arg Thr Val Leu Asn Asp Tyr Gly Tyr Asp Gln Asn Asn Glu Gly

260 265 270

Ile Phe Met Val Val Glu Pro Glu Asn Val Pro Tyr Ser Asn Ala Ser

275 280 285

Leu Phe Ser Tyr Phe Asp Ile Pro Ser Arg Asp Thr Leu Thr Leu Ser

290 295 300

Lys Tyr Ser Asp Ser Ile Ser Gln Cys Thr Thr Gln Ile Leu Lys Ala

305 310 315 320

Asn Ser Thr Phe Val Ala His Lys Tyr Ile Gly Ile Ile Ser Ser Glu

325 330 335

Tyr Asp Asn Lys Gln Pro Asp Asp Asn Ser Asn Gly Thr Ser Asn Glu

340 345 350

Ala Leu Lys Met Ser Asn Leu Glu Arg Ala Thr Ser Ile Val Leu Asp

355 360 365

Asn Lys Gly Asn Tyr Asp Ser Leu Ile Gln Ser His Lys Asn Ala Trp

370 375 380

Lys Arg Ile Tyr Lys Asp Ala Ser Ile Glu Ile Pro Ser Asp Gly Leu

385 390 395 400

Leu Glu Met Thr Ala Lys Ser Ser Ile Tyr His Leu Leu Ala Asn Thr

405 410 415

Arg Ser His Asn Val Ser Glu Asp Arg Gly Leu Pro Ile Gly Val Ser

420 425 430

Gly Leu Ser Ser Asp Ser Tyr Gly Gly Met Val Phe Trp Asp Ser Asp

435 440 445

Leu Trp Ile Leu Pro Ala Leu Leu Pro Phe Phe Pro Asn Ala Ala Arg

450 455 460

Gln Ile Asn Asn Tyr Arg Asn Ala Ser Leu His Gln Ala Lys Leu Asn

465 470 475 480

Ala Glu Lys Tyr Gly Tyr Asp Gly Ala Leu Tyr Pro Trp Thr Ser Gly

485 490 495

Arg Tyr Ala Asn Cys Thr Ser Thr Gly Pro Cys Val Asp Tyr Glu Tyr

500 505 510

His Ile Asn Val Asp Ile Ala Leu Ser Ser Phe Ala Ile Tyr Met Asn

515 520 525

Gly Asp Glu Asp Asp Glu Arg Ser Glu Glu Tyr Leu Arg Tyr Thr Thr

530 535 540

Trp Pro Phe Val Glu Asn Ala Ala Lys Met Phe Ala Gln Tyr Val Lys

545 550 555 560

Trp Asn Asp Thr Met Gln Gln Tyr Thr Thr His Asn Leu Thr Asp Pro

565 570 575

Asp Glu Tyr Ala Asn Phe Val Asp Asn Ala Ala Phe Thr Asn Ala Gly

580 585 590

Ile Gln Ser Val Met Val Trp Ala His Asp Ile Ala Arg His Leu Gly

595 600 605

Ile Asp Pro Asp Pro Gln Trp Leu Glu Ile Ala Asp Asn Ile His Ile

610 615 620

Pro Ile Ser Glu Thr Asn Ile Thr Leu Glu Tyr Ser Gly Met Asn Ser

625 630 635 640

Ser Val Glu Ile Lys Gln Ala Asp Val Val Leu Met Ile Tyr Pro Leu

645 650 655

Ser Tyr Ile Thr Asp Gln Ser Ile Leu Asn Asn Ala Ile Lys Asn Leu

660 665 670

Tyr Tyr Tyr Ser Glu Arg Gln Ser Ala Ser Gly Pro Ala Met Thr Tyr

675 680 685

Pro Val Phe Val Ala Gly Ala Ala Ser Leu Leu Asn Tyr Gly Ser Ser

690 695 700

Ser Gln Ser Tyr Leu Tyr Lys Ser Val Val Pro Tyr Leu Arg Ser Pro

705 710 715 720

Phe Ala Gln Phe Ser Glu Gln Ser Asp Asp Asn Phe Leu Thr Asn Gly

725 730 735

Leu Thr Gln Pro Ala Phe Pro Phe Leu Thr Ala Asn Gly Gly Phe Leu

740 745 750

Gln Ser Ile Leu Phe Gly Leu Thr Gly Leu Arg Tyr Ser Tyr Glu Val

755 760 765

Asp Glu Asp Thr Gly Lys Ile His Arg Leu Leu Lys Phe Asn Pro Ile

770 775 780

Gln Leu Pro Leu Leu Pro Gly Gly Ile Arg Ile Asn Asn Phe Lys Tyr

785 790 795 800

Met Gly Gln Val Leu Asp Val Leu Ile Thr Asp Thr Glu Gly Ile Ile

805 810 815

Ile His Lys Asn Gly Thr Lys Glu Ile Arg Ile Lys Val Pro Asp Arg

820 825 830

Thr Leu Ile Pro Asp Val Asp Val Lys Tyr Ser Glu Glu Thr Asp Pro

835 840 845

Ile Lys Gln Ile Leu His Gly Arg Arg Ser Val Pro Thr Gly Lys Asn

850 855 860

Tyr Phe Thr Ile Gln Pro Gly Asp Val Phe Lys Thr Pro Leu Tyr Ile

865 870 875 880

Pro Lys Lys Asn Leu Glu Gly Asn Leu Val Glu Ala Lys Gln Ile Thr

885 890 895

Asn Leu Thr Ala Gly Val Pro Gly Asp Val Ala Val Ser Val Ile Asp

900 905 910

Gly Asn Asn Phe Thr His Trp Gln Pro Ala Tyr Lys Asn Leu Pro Ala

915 920 925

Arg Leu Leu Ile Asp Met Gly Asn Asn Phe Thr Gln Glu Ile Lys Ser

930 935 940

Gly Lys Ile Ile Trp Gly Ser Arg Pro Ala Lys Thr Phe Ser Leu Ser

945 950 955 960

Ile Leu Pro Gln Thr Glu Glu Val Phe Lys Asn Leu Thr His Ile Leu

965 970 975

Ser Asn Val Asn Gln Tyr Cys Asn Lys Thr Gly Asp Glu Cys Val Arg

980 985 990

Asp Leu Glu Arg Thr Glu Lys Gly Phe Asp Ala Ala Ile Glu Asp Val

995 1000 1005

Phe Asp Trp Tyr Gly Ile Asp Pro Asp Ser Ile Ile Ser Thr His

1010 1015 1020

Pro Glu Leu Lys Asp Met Lys Thr Lys Phe Val Lys Ile Leu Asp

1025 1030 1035

His Tyr Lys Val Thr Pro Ser Glu Pro Tyr Pro Trp Arg Val Tyr

1040 1045 1050

Asn Glu Ser Gln Ile Val Leu Leu Pro Gly Asn Glu Thr Asp Phe

1055 1060 1065

Asp Ile Asp Tyr Ser Lys Val Ala Glu Met Asn Pro Glu Asn Val

1070 1075 1080

Asp Ile Asp Phe Arg Gly Asn Gln Thr Asp Trp Arg Lys Gly Arg

1085 1090 1095

Phe Ile Val Leu Thr Val His Asp Thr Tyr Asp Asp Asp Asp Asp

1100 1105 1110

Glu Lys Gly Ala Thr Ile Lys Glu Phe Ala Leu Phe Pro

1115 1120 1125

<210> 92

<211> 695

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体稻瘟病菌和酿酒酵母共同组成的序列

<400> 92

Met Lys Phe Ile Ser Thr Phe Leu Thr Phe Ile Leu Ala Ala Val Ser

1 5 10 15

Val Thr Ala Val Tyr Ile Asn Gly Ser Ile Ile Thr Pro Cys Asp Ser

20 25 30

Leu Ile Tyr Cys Arg Gly Glu Leu Leu Lys Glu Val Glu Leu Ala His

35 40 45

Pro Phe Ala Asp Ser Lys Thr Phe Val Asp Met Pro Thr Ile Lys Pro

50 55 60

Val Asp Glu Val Ile Glu Ala Phe Asn Lys Leu Gln Lys Pro Leu Ser

65 70 75 80

Asn Asn Thr Glu Leu Gln Asp Phe Leu Arg Glu Asn Phe Ala Gln Ala

85 90 95

Gly Gly Glu Leu Glu Glu Val Pro Asn Ser Glu Leu Glu Thr Asp Pro

100 105 110

Val Phe Leu Asp Lys Leu Asp Asp Thr Val Ile Arg Glu Phe Val Glu

115 120 125

Lys Val Ile Asp Ile Trp Pro Ser Leu Thr Arg Arg Tyr Lys Gly Pro

130 135 140

Ser Asn Cys Glu Ala Cys Ala Asp Ser Phe Ile Pro Val Asn Arg Thr

145 150 155 160

Phe Val Val Ala Gly Gly Arg Phe Arg Glu Pro Tyr Tyr Trp Asp Ser

165 170 175

Tyr Trp Ile Leu Glu Gly Leu Leu Arg Thr Gly Gly Ala Phe Thr Asn

180 185 190

Ile Ser Lys Asn Thr Val Glu Asn Phe Leu Asp Leu Val Glu Thr Ile

195 200 205

Gly Phe Val Pro Asn Gly Ala Arg Ile Tyr Tyr Lys Asn Arg Ser Gln

210 215 220

Pro Pro Leu Leu Ser Gln Met Val Arg Ile Tyr Val Glu His Thr Asn

225 230 235 240

Asp Thr Ser Ile Leu Gly Arg Ala Val Pro Leu Leu Ile Lys Glu His

245 250 255

Glu Phe Phe Ile Asn Asn Arg Ser Ile Asp Val Glu Ala Ser Asn Gly

260 265 270

Lys Thr Tyr Arg Leu Gln Arg Tyr Ala Val Thr Asn Thr Gln Pro Arg

275 280 285

Pro Glu Ser Tyr Arg Glu Asp Tyr Ile Thr Ala Ser Asn Arg Ser Tyr

290 295 300

Tyr Ser Pro Ser Gly Ile Ile Tyr Pro Glu Ser His Gln Leu Asn Glu

305 310 315 320

Ser Glu Lys Ala Val Leu Tyr Ser His Leu Ala Ser Gly Ala Glu Ser

325 330 335

Gly Trp Asp Tyr Thr Ser Arg Trp Leu Ser Thr Pro Ser Asp Ala Val

340 345 350

Arg Asp Asn Tyr Phe Pro Leu Arg Ser Leu Asn Thr Asn Asn Ile Val

355 360 365

Pro Val Asp Leu Asn Ser Ile Leu Tyr Ala Asn Glu Val Ala Ile Ala

370 375 380

Glu Phe Leu Asn Arg Thr Gly Asn Ser Thr Gly Ala Ser Asp Trp Met

385 390 395 400

Asp Leu Ala Lys Gln Arg Ser Glu Ala Met Tyr Ala Leu Met Trp Asn

405 410 415

Glu Thr Leu Trp Ser Tyr Phe Asp Tyr Asn Met Thr Ser Lys Thr Gln

420 425 430

Asn Arg Phe Ile Pro Val Asp Glu Asp Ala Val Ser Ile Glu Thr Asn

435 440 445

Asn Ala Pro Ala Gly Gln Gln Val Phe Phe His Val Ala Gln Tyr Tyr

450 455 460

Pro Phe Trp Thr Gly Ala Ala Pro Arg Ser Leu Lys Asn Asn Pro Leu

465 470 475 480

Ala Val Leu Arg Ala Tyr Glu Arg Ile Asp Ala Tyr Leu Asp Ile Lys

485 490 495

Arg Gly Ala Ile Pro Ala Thr Asn Leu Lys Thr Gly Gln Gln Trp Asp

500 505 510

Glu Pro Asn Val Trp Pro Pro Leu Met His Ile Leu Met Glu Gly Leu

515 520 525

Thr Arg Val Pro Ala Thr Phe Gly Glu Asp Asp Val Ala Trp Thr Glu

530 535 540

Ile Gln Asp Leu Ala Leu Arg Leu Gly Gln Arg Tyr Leu Asp Ser Thr

545 550 555 560

Phe Cys Thr Trp Tyr Ala Thr Gly Gly Ser Thr Ser Glu Thr Pro Gln

565 570 575

Leu Gln Gly Leu Asn Ala Glu Asp Lys Gly Ile Met Phe Glu Lys Tyr

580 585 590

Gly Asp Asn Ser Thr Asn Val Ala Gly Ser Gly Gly Glu Tyr Glu Val

595 600 605

Val Glu Gly Phe Gly Trp Thr Asn Gly Val Leu Met Trp Val Ala Asp

610 615 620

Thr Phe Asn Asn Lys Leu Thr Arg Pro Asp Cys Gly Asn Ile Thr Ala

625 630 635 640

Ala Asn Val His Ser Asp Gly Ser Gln Ala Arg Lys Arg Gly Glu Met

645 650 655

Trp Ser Ala Leu Glu Met His Pro Tyr Asp Ala Ala Trp Thr Lys Glu

660 665 670

Phe Gly Ala Arg Lys Val Arg Arg Asp Lys Ala Glu Ala Arg Ala Leu

675 680 685

Gly Asn Val Met Gly Gly Val

690 695

<210> 93

<211> 4884

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和小孢根霉共同组成的序列:

<400> 93

ctaaattcgg ccttgctcag agactcctgg attttggcta acaacgcagt cccttcgatg 60

catatagcta ggccacaaat tatgccaata acggtccatg ggttgatgtt ttcttgaatt 120

ctttcgtttt tcatgctatt tgcgtcttcc caagtcccag cgttccagta ttcatactgc 180

gcgttagagt ggtagccata agagccggca tattggtaat tttcagtatt aacgttagaa 240

cgtggtgaat acgatgtggt ccagccttgc ctcgttgtgt catatacgat ctttttcttt 300

gggtcacaaa gaatatcata tgcttgagag atgactttaa atctatgtag tttttcgctt 360

gatgttagca gcagcggtga tttactatca ctgttggtaa ccttttctga gctaaatatt 420

tgaatgttat cggaatggtc agggtggtac aattttacat aacgatgata tttttttttt 480

aacgacttct tgtccagttt aggatttcca gatccggcct ttggaatgcc aaaaatatca 540

tagggagttg gatctgccaa ctcaggccat tgttcatccc ttatcgtaag ttttctattg 600

ccatttttat cgttcgctgt agcatactta gctataaaag tgatttgtgg gggacacttt 660

tctacacatg ataagtgcca cttgaataaa aatgggtata cgaacttatg gtgtagcata 720

acaaatatat tgcaagtagt gacctatggt gtgtagatat acgtacagtt agttacgagc 780

ctaaagacac aacgtgtttg ttaattatac tgtcgctgta atatcttctc ttccattatc 840

accggtcatt ccttgcaggg gcggtagtac ccggagaccc tgaacttttc tttttttttt 900

tgcgaaatta aaaagttcat tttcaattcg acaatgagat ctacaagcca ttgttttatg 960

ttgatgagag ccagcttaaa gagttctcga gatctcccga gtttatcatt atcaatactg 1020

ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa 1080

aaaattggcc ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca 1140

cagaatatat aacatcatag gtgtctgggt gaacagttta ttcctggcat ccactaaata 1200

taatggagcc cgcttttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa 1260

tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca actacacaga 1320

acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcttg 1380

gagtaaatga tgacacaagg caattgacct acgcatgtat ctatctcatt ttcttacacc 1440

ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt 1500

tccctgaaat tattccccta tttgactaat aagtatataa agacggtagg tattgattgt 1560

aattctgtaa atctatttct taaacttctt aaattctact tttatagtta gtcttttttt 1620

tagtttaaaa caccaagaac ttagtttcga ataaacacac ataaacaaac aaatctagaa 1680

tgaaacttat gaatccatct atgaaggcat acgttttctt tatcttaagc tacttctctt 1740

tactcgttag ctcagctgcg gtgccaacct ctgccgccgt acaagttgag tcatacaatt 1800

atgacggtac cactttttca ggtagaatat tcgtcaaaaa cattgcctac tcaaaggtcg 1860

taacagttat ctactccgat ggatcagata actggaacaa taacaacaac aaagtttctg 1920

cagcttactc agaagcaatt tctgggtcta actacgaata ctggacattc tccgcaaagt 1980

tatccggaat taaacagttt tatgtcaaat acgaagtttc tggttcaaca tattacgaca 2040

acaacggtac caaaaactac caagtccaag caacctcagc gacatctaca acagctactg 2100

caaccacaac tacagctact ggcacaacaa ctacttctac aggtccaact agtactgcat 2160

ccgtatcatt ccctaccggt aactcaacaa tttcttcctg gataaaaaat caagaggaaa 2220

tcagccgttt tgctatgttg agaaatatca atccacctgg gtctgccaca gggttcatag 2280

ccgcatctct gtccacagcc ggcccagatt actattactc ttggactaga gattcagcac 2340

taacagctaa tgtgatcgct tacgaataca acacaacatt cactggaaac accacccttc 2400

ttaagtactt gaaagattac gttacatttt ctgtcaaaag ccaatctgta tctaccgttt 2460

gtaactgtct gggagaacca aagttcaacg ctgatggtag ttcttttaca ggtccatggg 2520

gcagaccaca aaacgacgga ccagcagaga gagctgttac ttttatgttg attgctgaca 2580

gctacttgac tcaaactaag gacgcatcct acgttaccgg tacattaaag ccagcaatct 2640

tcaaagatct tgattacgta gtttctgttt ggtctaacgg ttgctacgat ttatgggaag 2700

aggttaatgg tgttcatttc tatactctca tggtcatgag aaagggtttg atcttaggtg 2760

ccgacttcgc tgctagaaat ggtgactcta gtagagcttc aacctacaag caaactgcat 2820

caacaatgga atcaaagatc agttcttttt ggtcagattc taacaactac gtccaagttt 2880

ctcaatcagt taccgccgga gtgtcaaaaa agggactaga tgttagtaca ctattggcgg 2940

ccaacattgg tagtctgcct gatggctttt tcactccagg ctccgaaaag atattggcta 3000

cagcagtggc gttagaaaat gcattcgcat ccttgtaccc aattaactct aacctacctt 3060

cttacttggg taactcaatt ggaagatatc ctgaggatac atacaacggt aatggcaact 3120

ctcaggggaa tccatggttc cttgccgtca acgcatacgc agaactttac tacagagcta 3180

ttaaggaatg gattagtaat ggcaaggtga cagtatccaa tatctcacta cctttcttca 3240

aaaagtttga ttcttccgcc acttctggaa agacatacac tgctggtaca tcagatttca 3300

ataacttggc tcagaacatt gctttaggcg ccgatagatt cctgtctact gttaagttcc 3360

acgcatacac taacgggagt ctatcagaag agtacgatag atctaccggt atgagtactg 3420

gggctcgtga tttaacatgg tcccatgctt cattgatcac agtggcgtac gcaaaggccg 3480

gtagtcctgc agcttagtta attaaacagg ccccttttcc tttgtcgata tcatgtaatt 3540

agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg 3600

agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag 3660

aacgttattt atatttcaaa tttttctttt ttttctgtac aaacgcgtgt acgcatgtaa 3720

cgggcagacg gccggccata acttcgtata atgtatgcta tacgaagtta tggcaacggt 3780

tcatcatctc atggatctgc acatgaacaa acaccagagt caaacgacgt tgaaattgag 3840

gctactgcgc caattgatga caatacagac gatgataaca aaccgaagtt atctgatgta 3900

gaaaaggatt agagatgcta agagatagtg atgatatttc ataaataatg taattctata 3960

tatgttaatt accttttttg cgaggcatat ttatggtgaa ggataagttt tgaccatcaa 4020

agaaggttaa tgtggctgtg gtttcagggt ccataaagct tttcaattca tctttttttt 4080

ttttgttctt ttttttgatt ccggtttctt tgaaattttt ttgattcggt aatctccgag 4140

cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtggt 4200

gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc 4260

aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct 4320

agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct 4380

tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa 4440

atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt 4500

aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt 4560

gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa 4620

tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag 4680

caggcggcgg aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca 4740

tgcaagggct ccctagctac tggagaatat actaagggta ctgttgacat tgcgaagagc 4800

gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt 4860

tacgattggt tgattatgac acgc 4884

<210> 94

<211> 1790

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 94

ggaagagctc ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta 60

tctgatgtag aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt 120

aattctatat atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt 180

gaccatcaaa gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat 240

cttttttttt ttgttctttt ttttgattcc ggtttctttg aaattttttt gattcggtaa 300

tctccgagca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca 360

tatgtggtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa 420

aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta 480

ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact 540

tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag 600

gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg 660

gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca 720

gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa 780

tagcagaatg ggcagacatt acgaatgcgc acggtgtggt gggcccaggt attgttagcg 840

gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg atgttagcag 900

aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact gttgacattg 960

cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag 1020

atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg 1080

cattgggtca acagtataga gccgtggatg atgtggtctc tacaggatct gacattatta 1140

ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca 1200

gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat 1260

tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt 1320

tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa aaattggaaa 1380

gaaaaagctt catggccttt ataaaaagga accatccaat acctcgccag aaccaagtaa 1440

cagtatttta cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcatt 1500

gaactccaaa gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt 1560

catgcgtttg ataacttcgt ataatgtatg ctatacgaag ttatgcggcc gccagcacgc 1620

agcacgctgt atttacgtat ttaattttat atatttgtgc atacactact agggaagact 1680

tgaaaaaaac ctaggaaatg aaaaaacgac acaggaagtc ccgtatttac tattttttcc 1740

ttccttttga tggggcaggg cggaaataga ggataggata agcctactgc 1790

<210> 95

<211> 605

<212> PRT

<213> 小孢根霉(Rhizopus microsporus)

<400> 95

Met Lys Leu Met Asn Pro Ser Met Lys Ala Tyr Val Phe Phe Ile Leu

1 5 10 15

Ser Tyr Phe Ser Leu Leu Val Ser Ser Ala Ala Val Pro Thr Ser Ala

20 25 30

Ala Val Gln Val Glu Ser Tyr Asn Tyr Asp Gly Thr Thr Phe Ser Gly

35 40 45

Arg Ile Phe Val Lys Asn Ile Ala Tyr Ser Lys Val Val Thr Val Ile

50 55 60

Tyr Ser Asp Gly Ser Asp Asn Trp Asn Asn Asn Asn Asn Lys Val Ser

65 70 75 80

Ala Ala Tyr Ser Glu Ala Ile Ser Gly Ser Asn Tyr Glu Tyr Trp Thr

85 90 95

Phe Ser Ala Lys Leu Ser Gly Ile Lys Gln Phe Tyr Val Lys Tyr Glu

100 105 110

Val Ser Gly Ser Thr Tyr Tyr Asp Asn Asn Gly Thr Lys Asn Tyr Gln

115 120 125

Val Gln Ala Thr Ser Ala Thr Ser Thr Thr Ala Thr Ala Thr Thr Thr

130 135 140

Thr Ala Thr Gly Thr Thr Thr Thr Ser Thr Gly Pro Thr Ser Thr Ala

145 150 155 160

Ser Val Ser Phe Pro Thr Gly Asn Ser Thr Ile Ser Ser Trp Ile Lys

165 170 175

Asn Gln Glu Glu Ile Ser Arg Phe Ala Met Leu Arg Asn Ile Asn Pro

180 185 190

Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser Thr Ala Gly

195 200 205

Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu Thr Ala Asn

210 215 220

Val Ile Ala Tyr Glu Tyr Asn Thr Thr Phe Thr Gly Asn Thr Thr Leu

225 230 235 240

Leu Lys Tyr Leu Lys Asp Tyr Val Thr Phe Ser Val Lys Ser Gln Ser

245 250 255

Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Ala Asp

260 265 270

Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro

275 280 285

Ala Glu Arg Ala Val Thr Phe Met Leu Ile Ala Asp Ser Tyr Leu Thr

290 295 300

Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys Pro Ala Ile

305 310 315 320

Phe Lys Asp Leu Asp Tyr Val Val Ser Val Trp Ser Asn Gly Cys Tyr

325 330 335

Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Val

340 345 350

Met Arg Lys Gly Leu Ile Leu Gly Ala Asp Phe Ala Ala Arg Asn Gly

355 360 365

Asp Ser Ser Arg Ala Ser Thr Tyr Lys Gln Thr Ala Ser Thr Met Glu

370 375 380

Ser Lys Ile Ser Ser Phe Trp Ser Asp Ser Asn Asn Tyr Val Gln Val

385 390 395 400

Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys Gly Leu Asp Val Ser

405 410 415

Thr Leu Leu Ala Ala Asn Ile Gly Ser Leu Pro Asp Gly Phe Phe Thr

420 425 430

Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Leu Glu Asn Ala

435 440 445

Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu Pro Ser Tyr Leu Gly

450 455 460

Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn

465 470 475 480

Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn Ala Tyr Ala Glu Leu

485 490 495

Tyr Tyr Arg Ala Ile Lys Glu Trp Ile Ser Asn Gly Lys Val Thr Val

500 505 510

Ser Asn Ile Ser Leu Pro Phe Phe Lys Lys Phe Asp Ser Ser Ala Thr

515 520 525

Ser Gly Lys Thr Tyr Thr Ala Gly Thr Ser Asp Phe Asn Asn Leu Ala

530 535 540

Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu Ser Thr Val Lys Phe

545 550 555 560

His Ala Tyr Thr Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Ser Thr

565 570 575

Gly Met Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu

580 585 590

Ile Thr Val Ala Tyr Ala Lys Ala Gly Ser Pro Ala Ala

595 600 605

<210> 96

<211> 4474

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母、小孢根霉和构巢曲霉共同组成的序列:

<400> 96

gtgtttgtta attatactgt cgctgtaata tcttctcttc cattatcacc ggtcattcct 60

tgcaggggcg gtagtacccg gagaccctga acttttcttt ttttttttgc gaaattaaaa 120

agttcatttt caattcgaca atgagatcta caagccattg ttttatgttg atgagagcca 180

gcttaaagag ttctcgagat ctcccgagtt tatcattatc aatactgcca tttcaaagaa 240

tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa attggccttt 300

taattctgct gtaacccgta catgcccaaa atagggggcg ggttacacag aatatataac 360

atcataggtg tctgggtgaa cagtttattc ctggcatcca ctaaatataa tggagcccgc 420

tttttttaag ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc 480

accaaccatc agttcatagg tccattctct tagcgcaact acacagaaca ggggcacaaa 540

caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac ctgcttggag taaatgatga 600

cacaaggcaa ttgacctacg catgtatcta tctcattttc ttacaccttc tattaccttc 660

tgctctctct gatttggaaa aagctgaaaa aaaaggttga aaccagttcc ctgaaattat 720

tcccctattt gactaataag tatataaaga cggtaggtat tgattgtaat tctgtaaatc 780

tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag tttaaaacac 840

caagaactta gtttcgaata aacacacata aacaaacaaa tctagaatga aacttatgaa 900

tccatctatg aaggcatacg ttttctttat cttaagctac ttctctttac tcgttagctc 960

agctgcggtg ccaacctctg ccgccgtaca agttgagtca tacaattatg acggtaccac 1020

tttttcaggt agaatattcg tcaaaaacat tgcctactca aaggtcgtaa cagttatcta 1080

ctccgatgga tcagataact ggaacaataa caacaacaaa gtttctgcag cttactcaga 1140

agcaatttct gggtctaact acgaatactg gacattctcc gcaaagttat ccggaattaa 1200

acagttttat gtcaaatacg aagtttctgg ttcaacatat tacgacaaca acggtaccaa 1260

aaactaccaa gtccaagcaa cctcagcgac atctacaaca gctactgcaa ccacaactac 1320

agctactggc acaacaacta cttctacagg tccaactagt actgcatccg tatcattccc 1380

taccggtaac tcaacaattt cttcctggat aaaaaatcaa gaggaaatca gccgttttgc 1440

tatgttgaga aatatcaatc cacctgggtc tgccacaggg ttcatagccg catctctgtc 1500

cacagccggc ccagattact attactcttg gactagagat tcagcactaa cagctaatgt 1560

gatcgcttac gaatacaaca caacattcac tggaaacacc acccttctta agtacttgaa 1620

agattacgtt acattttctg tcaaaagcca atctgtatct accgtttgta actgtctggg 1680

agaaccaaag ttcaacgctg atggtagttc ttttacaggt ccatggggca gaccacaaaa 1740

cgacggacca gcagagagag ctgttacttt tatgttgatt gctgacagct acttgactca 1800

aactaaggac gcatcctacg ttaccggtac attaaagcca gcaatcttca aagatcttga 1860

ttacgtagtt tctgtttggt ctaacggttg ctacgattta tgggaagagg ttaatggtgt 1920

tcatttctat actctcatgg tcatgagaaa gggtttgatc ttaggtgccg acttcgctgc 1980

tagaaatggt gactctagta gagcttcaac ctacaagcaa actgcatcaa caatggaatc 2040

aaagatcagt tctttttggt cagattctaa caactacgtc caagtttctc aatcagttac 2100

cgccggagtg tcaaaaaagg gactagatgt tagtacacta ttggcggcca acattggtag 2160

tctgcctgat ggctttttca ctccaggctc cgaaaagata ttggctacag cagtggcgtt 2220

agaaaatgca ttcgcatcct tgtacccaat taactctaac ctaccttctt acttgggtaa 2280

ctcaattgga agatatcctg aggatacata caacggtaat ggcaactctc aggggaatcc 2340

atggttcctt gccgtcaacg catacgcaga actttactac agagctatta aggaatggat 2400

tagtaatggc aaggtgacag tatccaatat ctcactacct ttcttcaaaa agtttgattc 2460

ttccgccact tctggaaaga catacactgc tggtacatca gatttcaata acttggctca 2520

gaacattgct ttaggcgccg atagattcct gtctactgtt aagttccacg catacactaa 2580

cgggagtcta tcagaagagt acgatagatc taccggtatg agtactgggg ctcgtgattt 2640

aacatggtcc catgcttcat tgatcacagt ggcgtacgca aaggccggta gtcctgcagc 2700

ttagttaatt aaacaggccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc 2760

ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct 2820

gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata 2880

tttcaaattt ttcttttttt tctgtacaaa cgcgtgtacg catgtaacgg gcagacggcc 2940

ggccataact tcgtataatg tatgctatac gaagttatcc ttacatcaca cccaatcccc 3000

cacaagtgat cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc 3060

agattttctc ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact 3120

aaatttcccc tctttcttcc tctagggtgg cgttaattac ccgtactaaa ggtttggaaa 3180

agaaaaaaga gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat 3240

cacgtttctt tttcttgaaa aatttttttt ttgatttttt tctctttcga tgacctccca 3300

ttgatattta agttaataaa tggtcttcaa tttctcaagt ttcagtttcg tttttcttgt 3360

tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa 3420

gttttaatta caaaatgcca caatcctggg aagaattggc cgccgacaaa cgtgcccgtt 3480

tggctaaaac cattcctgac gaatggaagg ttcaaacttt gcctgccgaa gattccgtta 3540

ttgatttccc aaagaagtcc ggtattttgt ctgaggctga attgaagatt accgaagcct 3600

ctgctgctga tttggtctcc aagttggccg ctggtgagtt gacttctgtt gaagtcactt 3660

tggctttttg taagagagct gctattgctc aacaattaac caactgtgct cacgaattct 3720

tcccagatgc tgctttagct caagctagag aattagatga atactacgct aagcataaga 3780

gaccagttgg tccattacac ggtttaccaa tctctttaaa ggaccaattg cgtgttaagg 3840

gttacgaaac ctccatgggt tacatttcct ggttaaacaa atacgatgaa ggtgattccg 3900

tcttaaccac catgttgaga aaagctggtg ctgttttcta cgttaagacc tctgtcccac 3960

aaaccttgat ggtctgtgaa accgtcaaca acatcattgg tagaactgtc aatccaagaa 4020

acaaaaattg gtcctgtggt ggttcttctg gtggtgaagg tgctattgtt ggtattagag 4080

gtggtgttat tggtgtcggt actgacattg gtggttccat tagagtccca gctgctttca 4140

actttttata cggtttgaga ccatctcacg gtagattgcc atatgctaaa atggctaact 4200

ctatggaagg tcaagaaacc gttcactccg tcgttggtcc tatcactcac tccgtcgaag 4260

acttgagatt gttcaccaaa tctgtcttgg gtcaagaacc ttggaagtac gactctaagg 4320

tcatccccat gccatggaga caatctgaat ctgacatcat tgcctctaag attaagaatg 4380

gtggtttgaa cattggttat tacaatttcg acggtaacgt cttgccacac ccaccaattt 4440

tacgtggtgt cgaaactacc gttgccgctt tggc 4474

<210> 97

<211> 1878

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和构巢曲霉共同组成的序列:

<400> 97

gaagattacc gaagcctctg ctgctgattt ggtctccaag ttggccgctg gtgagttgac 60

ttctgttgaa gtcactttgg ctttttgtaa gagagctgct attgctcaac aattaaccaa 120

ctgtgctcac gaattcttcc cagatgctgc tttagctcaa gctagagaat tagatgaata 180

ctacgctaag cataagagac cagttggtcc attacacggt ttaccaatct ctttaaagga 240

ccaattgcgt gttaagggtt acgaaacctc catgggttac atttcctggt taaacaaata 300

cgatgaaggt gattccgtct taaccaccat gttgagaaaa gctggtgctg ttttctacgt 360

taagacctct gtcccacaaa ccttgatggt ctgtgaaacc gtcaacaaca tcattggtag 420

aactgtcaat ccaagaaaca aaaattggtc ctgtggtggt tcttctggtg gtgaaggtgc 480

tattgttggt attagaggtg gtgttattgg tgtcggtact gacattggtg gttccattag 540

agtcccagct gctttcaact ttttatacgg tttgagacca tctcacggta gattgccata 600

tgctaaaatg gctaactcta tggaaggtca agaaaccgtt cactccgtcg ttggtcctat 660

cactcactcc gtcgaagact tgagattgtt caccaaatct gtcttgggtc aagaaccttg 720

gaagtacgac tctaaggtca tcccaatgcc atggagacaa tctgaatctg acatcattgc 780

ctctaagatt aagaatggtg gtttgaacat tggttattac aatttcgacg gtaacgtctt 840

gccacaccca ccaattttac gtggtgtcga aactaccgtt gccgctttgg ccaaggctgg 900

tcacaccgtt actccatgga ctccatacaa gcatgatttc ggtcatgact tgatttccca 960

catctatgct gctgatggtt ctgccgacgt catgagagac atttctgcct ctggtgagcc 1020

agccatccct aacattaagg acttgttgaa cccaaatatt aaggctgtta acatgaacga 1080

attgtgggac actcatttac aaaagtggaa ctatcaaatg gaatacttgg aaaagtggcg 1140

tgaagctgaa gaaaaagctg gtaaggaatt ggacgctatt atcgctccaa ttactcctac 1200

cgccgctgtc agacacgatc aattcagata ctacggttac gcctccgtta ttaacttatt 1260

ggatttcacc tctgttgtcg tcccagtcac tttcgctgat aagaatattg ataagaagaa 1320

cgaatctttt aaagctgttt ccgaattgga tgctttggtt caagaagaat acgacccaga 1380

ggcttatcac ggtgctcctg ttgctgttca agttattggt agaagattgt ccgaagagag 1440

aactttggct atcgccgaag aagtcggtaa attgttgggt aacgtcgtca ctccataagg 1500

agattgataa gacttttcta gttgcatatc ttttatattt aaatcttatc tattagttaa 1560

ttttttgtaa tttatcctta tatatagtct ggttattcta aaatatcatt tcagtatcta 1620

aaaattcccc tcttttttca gttatatctt aacaggcgat aacttcgtat aatgtatgct 1680

atacgaagtt atgcggccgc cagcacgcag cacgctgtat ttacgtattt aattttatat 1740

atttgtgcat acactactag ggaagacttg aaaaaaacct aggaaatgaa aaaacgacac 1800

aggaagtccc gtatttacta ttttttcctt ccttttgatg gggcagggcg gaaatagagg 1860

ataggataag cctactgc 1878

<210> 98

<211> 8719

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和P1噬菌体共同组成的序列:

<400> 98

atcacatagg aagcaacagg cgcgttggac ttttaatttt cgaggaccgc gaatccttac 60

atcacaccca atcccccaca agtgatcccc cacacaccat agcttcaaaa tgtttctact 120

ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac 180

ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt 240

actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg 300

caataaaaat ttttatcacg tttctttttc ttgaaaattt ttttttttga tttttttctc 360

tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc tcaagtttca 420

gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag aaagaaagca 480

tagcaatcta atctaagttt taattacaaa tctagaatga gtgaatctcc aatgttcgct 540

gccaacggca tgccaaaggt aaatcaaggt gctgaagaag atgtcagaat tttaggttac 600

gacccattag cttctccagc tctccttcaa gtgcaaatcc cagccacacc aacttctttg 660

gaaactgcca agagaggtag aagagaagct atagatatta ttaccggtaa agacgacaga 720

gttcttgtca ttgtcggtcc ttgttccatc catgatcttg aagccgctca agaatacgct 780

ttgagattaa agaaattgtc agatgaatta aaaggtgatt tatccatcat tatgagagca 840

tacttggaga agccaagaac aaccgtcggc tggaaaggtc taattaatga ccctgatgtt 900

aacaacactt tcaacatcaa caagggtttg caatccgcta gacaattgtt tgtcaacttg 960

acaaatatcg gtttgccaat tggttctgaa atgcttgata ccatttctcc taaatacttg 1020

gctgatttgg tctccttcgg tgccattggt gccagaacca ccgaatctca actgcacaga 1080

gaattggcct ccggtttgtc tttcccagtt ggtttcaaga acggtaccga tggtacctta 1140

aatgttgctg tggatgcttg tcaagccgct gctcattctc accatttcat gggtgttact 1200

aagcatggtg ttgctgctat caccactact aagggtaacg aacactgctt cgttattcta 1260

agaggtggta aaaagggtac caactacgac gctaagtccg ttgcagaagc taaggctcaa 1320

ttgcctgccg gttccaacgg tctaatgatt gactactctc acggtaactc caataaggat 1380

ttcagaaacc aaccaaaggt caatgacgtt gtttgtgagc aaatcgctaa cggtgaaaac 1440

gccattaccg gtgtcatgat tgaatcaaac atcaacgaag gtaaccaagg catcccagcc 1500

gaaggtaaag ccggcttgaa atatggtgtt tccatcactg atgcttgtat aggttgggaa 1560

actactgaag acgtcttgag gaaattggct gctgctgtca gacaaagaag agaagttaac 1620

aagaaataga tgttttttta atgatatatg taacgtacat tctttcctct accactgcca 1680

attcggtatt atttaattgt gtttagcgct atttactaat taactagaaa ctcaattttt 1740

aaaggcaaag ctcgctgacc tttcactgat ttcgtggatg ttatactatc agttactctt 1800

ctgcaaaaaa aaattgagtc atatcgtagc tttgggatta tttttctctc tctccacggc 1860

taattaggtg atcatgaaaa aatgaaaaat tcatgagaaa agagtcagac atcgaaacat 1920

acataagttg atattccttt gatatcgacg actactcaat caggttttaa aagaaaagag 1980

gcagctattg aagtagcagt atccagttta ggttttttaa ttatttacaa gtaaagaaaa 2040

agagaatgcc ggtcgttcac ggcggccgcg ccagaaaaag gaagtgtttc cctccttctt 2100

gaattgatgt taccctcata aagcacgtgg cctcttatcg agaaagaaat taccgtcgct 2160

cgtgatttgt ttgcaaaaag aacaaaactg aaaaaaccca gacacgctcg acttcctgtc 2220

ttcctattga ttgcagcttc caatttcgtc acacaacaag gtcctagcga cggctcacag 2280

gttttgtaac aagcaatcga aggttctgga atggcgggaa agggtttagt accacatgct 2340

atgatgccca ctgtgatctc cagagcaaag ttcgttcgat cgtactgtta ctctctctct 2400

ttcaaacaga attgtccgaa tcgtgtgaca acaacagcct gttctcacac actcttttct 2460

tctaaccaag ggggtggttt agtttagtag aacctcgtga aacttacatt tacatatata 2520

taaacttgca taaattggtc aatgcaagaa atacatattt ggtcttttct aattcgtagt 2580

ttttcaagtt cttagatgct ttctttttct cttttttaca gatcatcaac tcttttttac 2640

agatcatcaa ggaagtaatt atctactttt tacaagaatt catgtctaat ttacttactg 2700

ttcaccaaaa cttgcctgca ttaccagttg acgcaacctc cgatgaagtc agaaagaacc 2760

ttatggatat gtttagagat agacaagctt tctccgaaca tacttggaaa atgttattat 2820

ccgtttgtag atcctgggcc gcttggtgta aacttaacaa tagaaaatgg tttcctgctg 2880

aaccagaaga cgtcagagat tacttacttt acttacaagc tagaggtttg gctgttaaaa 2940

ctatccaaca acacttaggt caattgaata tgttacacag aagatccggt ttaccaagac 3000

catccgattc caacgcagtt tcccttgtta tgagaagaat tagaaaagaa aatgttgacg 3060

ctggtgaaag agctaaacaa gcattagcat ttgaaagaac cgatttcgat caagttagat 3120

ccttaatgga aaattccgat agatgtcaag atattagaaa cttagctttc ttaggtattg 3180

cttacaacac attattaaga atcgctgaaa ttgctagaat tagagttaaa gatatttcaa 3240

gaaccgatgg cggtagaatg ttaatccaca ttggcagaac aaaaacctta gtctccacag 3300

caggcgtcga aaaagcatta tcattaggtg ttactaaatt agttgaacgt tggatttccg 3360

tttccggtgt tgcagatgac ccaaacaact acttattctg tcgtgttaga aaaaatggtg 3420

ttgccgctcc ttccgctacc tcacaattat ccacaagagc attagaaggc atttttgaag 3480

ctacccacag acttatttat ggtgcaaaag acgattccgg tcaaagatat ttagcttggt 3540

ctggtcattc cgctagagtt ggtgccgcaa gagacatggc aagagctggt gtttctattc 3600

ctgaaattat gcaagccggt ggttggacta atgttaacat tgttatgaac tatatcagaa 3660

acttagattc cgaaacaggt gctatggtta gattacttga agacggtgat taagctagct 3720

aagatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta 3780

tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt 3840

tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt 3900

gggacgctcg aaggagctcc aattcgccct atagtgagtc gtattacaat tcactggccg 3960

tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 4020

cacatccccc cttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 4080

aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg 4140

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 4200

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 4260

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 4320

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 4380

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 4440

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 4500

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt 4560

ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcag 4620

ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt 4680

acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct 4740

gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt 4800

ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga 4860

cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa 4920

ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg 4980

ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat 5040

gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc 5100

ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat 5160

tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca 5220

aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta 5280

actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt 5340

ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa 5400

gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta 5460

ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg 5520

caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca 5580

ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt 5640

tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa 5700

aaaaatgatg aattgaattg aaaagcgtgg tgcactctca gtacaatctg ctctgatgcc 5760

gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 5820

ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 5880

aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 5940

ttataggtta atgtcatgat aataatggtt tcttaggacg gatcgcttgc ctgtaactta 6000

cacgcgcctc gtatctttta atgatggaat aatttgggaa tttactctgt gtttatttat 6060

ttttatgttt tgtatttgga ttttagaaag taaataaaga aggtagaaga gttacggaat 6120

gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata 6180

tatttattag acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa 6240

tgtaaaatca caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc 6300

attaatacct gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta 6360

gagtctttta catcttcgga aaacaaaaac tattttttct ttaatttctt tttttacttt 6420

ctatttttaa tttatatatt tatattaaaa aatttaaatt ataattattt ttatagcacg 6480

tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 6540

atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 6600

tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 6660

cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 6720

agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 6780

taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 6840

tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 6900

catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 6960

ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 7020

ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt tttttcacaa 7080

catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 7140

aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 7200

aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 7260

taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 7320

atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 7380

gccctcccgt atcgtagtta tctacacgac gggcagtcag gcaactatgg atgaacgaaa 7440

tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 7500

ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 7560

gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 7620

agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 7680

aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 7740

agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 7800

tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 7860

atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 7920

taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 7980

gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 8040

gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 8100

aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggga acgcctggta 8160

tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 8220

gtcagggggg ccgagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 8280

cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 8340

ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 8400

cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 8460

ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 8520

gcgcaacgca attaatgtga gttacctcac tcattaggca ccccaggctt tacactttat 8580

gcttccggct cctatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 8640

ctatgaccat gattacgcca agctcggaat taaccctcac taaagggaac aaaagctggg 8700

taccgggccc cccctcgag 8719

<210> 99

<211> 1632

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 99

ggcaacggtt catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt 60

gaaattgagg ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta 120

tctgatgtag aaaaggatta gagatgctaa gagatagtga tgatatttca taaataatgt 180

aattctatat atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt 240

gaccatcaaa gaaggttaat gtggctgtgg tttcagggtc cataaagctt ttcaattcat 300

cttttttttt tttgttcttt tttttgattc cggtttcttt gaaatttttt tgattcggta 360

atctccgagc agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc 420

atatgtggtg ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca 480

aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct 540

actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac 600

ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta 660

ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag 720

ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac 780

agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga 840

atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc 900

ggtttgaagc aggcggcgga agaagtaaca aaggaaccta gaggcctttt gatgttagca 960

gaattgtcat gcaagggctc cctagctact ggagaatata ctaagggtac tgttgacatt 1020

gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga 1080

gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac 1140

gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt 1200

attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac 1260

agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta 1320

ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag 1380

ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga 1440

aagaaaaagc ttcatggcct ttataaaaag gaaccatcca atacctcgcc agaaccaagt 1500

aacagtattt tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca 1560

ttgaactcca aagaacaaca agagttccaa aaagtagtgg aacaaaagca aatgaaggat 1620

ttcatgcgtt tg 1632

<210> 100

<211> 4158

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和蜡样芽孢杆菌共同组成的序列:

<400> 100

tgagctccgg gtgggaggaa ggcgcggcaa ttagaatgtg tgggtgcgga agctcgccgc 60

tcccatcaag agagtggaag acgtatggtc tgggtgcgaa gtaccaccac gtttcttttt 120

catctcttaa gtgggattct tacgaaacac gtcacagggt caaaagaaag agaacaaaag 180

caatattgta attgtctcag tccacggcaa tgacatggca tggccccgaa ggcttttttt 240

gtctgtcttc cttgggtctt accccgccac gcgttaatag tgagacaagc aggaaatccg 300

tatcattttc tcgcatacac gaacccgcgt gcgcctggta aattgcagga ttctcattgt 360

ccggttttct ttatgggaat aatcatcatc accattatca ctgttactct tgcgatcatc 420

atcattaaca taattttttt aacgctgttt gatgatggta tgtgctttta ttgttcctta 480

ctcacctttt cctttgtgtc ttttaatttt gaccattttg accattttga cctttgatga 540

tgtgtgagtt cctcttttct ttttttcttt tcttttttcc tttttttttc ttttcttact 600

gtgttaatca ctttctttcc tttttgttca tattgtcgtc ttgttcattt tcgttcaatt 660

gataatgtat ataaatcttt cgtaagtatc tcttgattgc catttttttc tttccaagtt 720

tccttgttct cgaggccaga aaaaggaagt gtttccctcc ttcttgaatt gatgttaccc 780

tcataaagca cgtggcctct tatcgagaaa gaaattaccg tcgctcgtga tttgtttgca 840

aaaagaacaa aactgaaaaa acccagacac gctcgacttc ctgtcttcct attgattgca 900

gcttccaatt tcgtcacaca acaaggtcct agcgacggct cacaggtttt gtaacaagca 960

atcgaaggtt ctggaatggc gggaaagggt ttagtaccac atgctatgat gcccactgtg 1020

atctccagag caaagttcgt tcgatcgtac tgttactctc tctctttcaa acagaattgt 1080

ccgaatcgtg tgacaacaac agcctgttct cacacactct tttcttctaa ccaagggggt 1140

ggtttagttt agtagaacct cgtgaaactt acatttacat atatataaac ttgcataaat 1200

tggtcaatgc aagaaataca tatttggtct tttctaattc gtagtttttc aagttcttag 1260

atgctttctt tttctctttt ttacagatca tcaaggaagt aattatctac tttttacaag 1320

tctagaatga caacatcaaa tacctacaaa ttctatctaa acggtgaatg gagagaatct 1380

tcctctggag aaactattga gataccatca ccatacttac atgaagtgat cggacaggtt 1440

caagcaatca ctagaggaga ggttgacgaa gcgattgcta gcgctaagga agcacagaaa 1500

tcttgggctg aggcatctct acaagataga gctaagtact tgtacaaatg ggcagatgaa 1560

ttggtaaaca tgcaagacga aatcgccgat atcatcatga aggaagtggg caagggttac 1620

aaagacgcta aaaaggaggt tgttagaacc gccgatttca tcagatacac cattgaagag 1680

gcactccata tgcacggtga atccatgatg ggcgattcat ttcctggtgg aacaaaatct 1740

aagctagcaa taatccaaag agcgcctctg ggtgtagtct tagccatcgc tccattcaat 1800

taccctgtaa acctttctgc tgcaaaattg gcaccagcct taattatggg taacgctgtg 1860

atattcaagc cagcaactca gggtgctatt tccggcatca aaatggttga agctttgcat 1920

aaggctggtt tgccaaaggg tttggttaac gttgccacag gtagaggtag cgtcataggc 1980

gattatttgg tcgaacacga agggataaac atggtttcct tcaccggtgg cactaacact 2040

ggtaagcatt tagcaaaaaa ggcctcaatg attccattag tcttggaact tggtggcaaa 2100

gatccaggca tcgttcgtga agatgcagac ctacaagatg ctgcgaatca tatcgtatct 2160

ggtgcgttca gttactcagg gcagagatgt acagccatta agagagtcct tgttcatgaa 2220

aatgttgctg atgaactggt atcattggtt aaggaacaag tggcaaagct ttctgtggga 2280

tcaccagagc aagattcaac aattgttcct ctgattgacg ataagtccgc tgattttgtt 2340

cagggtttag tggacgatgc agtcgaaaag ggcgctacaa ttgtcattgg gaacaagaga 2400

gaacgtaacc taatctaccc aacattgatt gatcacgtca cagaggaaat gaaagttgcc 2460

tgggaggaac cattcggtcc tattcttcca attattagag ttagtagcga cgagcaagct 2520

attgaaattg caaataagag tgagttcgga ttacaagctt ctgtgtttac caaagacata 2580

aacaaggcat tcgcaatcgc aaataagatt gagactggtt cagtgcaaat caacggtaga 2640

acagagagag gaccagatca ctttcctttt atcggggtta agggatctgg gatgggtgcc 2700

caaggcatca gaaagtcttt ggaatctatg actagagaaa aagttactgt cttaaatctc 2760

gtatgattaa acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt 2820

acattcacgc cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga 2880

agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt 2940

tcaaattttt cttttttttc tgtacaaacg cgtgtacgca tgtaacgggc agacggccgg 3000

ccataacttc gtataatgta tgctatacga agttatggca acggttcatc atctcatgga 3060

tctgcacatg aacaaacacc agagtcaaac gacgttgaaa ttgaggctac tgcgccaatt 3120

gatgacaata cagacgatga taacaaaccg aagttatctg atgtagaaaa ggattagaga 3180

tgctaagaga tagtgatgat atttcataaa taatgtaatt ctatatatgt taattacctt 3240

ttttgcgagg catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg 3300

ctgtggtttc agggtccata aagcttttca attcatcttt tttttttttg ttcttttttt 3360

tgattccggt ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga 3420

aggaaggagc acagacttag attggtatat atacgcatat gtggtgttga agaaacatga 3480

aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa 3540

atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc 3600

aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt 3660

accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa 3720

acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca 3780

ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat 3840

acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg 3900

aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcggaagaa 3960

gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta 4020

gctactggag aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt 4080

atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt 4140

atgacacgcg gccgcggc 4158

<210> 101

<211> 1127

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 101

gctccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac 60

tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg 120

gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag 180

gtattgttag cggtttgaag caggcggcgg aagaagtaac aaaggaacct agaggccttt 240

tgatgttagc agaattgtca tgcaagggct ccctagctac tggagaatat actaagggta 300

ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca 360

tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg 420

acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat 480

ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg 540

gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact 600

aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt 660

aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa 720

aaaaaattgg aaagaaaaag cttcatggcc tttataaaaa ggaaccatcc aatacctcgc 780

cagaaccaag taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa 840

agatggacgc attgaactcc aaagaacaac aagagttcca aaaagtagtg gaacaaaagc 900

aaatgaagga tttcatgcgt ttgataactt cgtataatgt atgctatacg aagttatctc 960

gaggataaaa ctactacgct aaaaataaaa taaaaatgta tgatttccct ccatttccga 1020

ccaattgtat aattttatat ctgcatgact taataatata atataatact tataaaatac 1080

gaatagaaaa atttaaaccg atgtaatgca tccttttctt tgttgtc 1127

<210> 102

<211> 4542

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母、蜡样芽孢杆菌和构巢曲霉共同组成的序列:

<400> 102

tgagctccgg gtgggaggaa ggcgcggcaa ttagaatgtg tgggtgcgga agctcgccgc 60

tcccatcaag agagtggaag acgtatggtc tgggtgcgaa gtaccaccac gtttcttttt 120

catctcttaa gtgggattct tacgaaacac gtcacagggt caaaagaaag agaacaaaag 180

caatattgta attgtctcag tccacggcaa tgacatggca tggccccgaa ggcttttttt 240

gtctgtcttc cttgggtctt accccgccac gcgttaatag tgagacaagc aggaaatccg 300

tatcattttc tcgcatacac gaacccgcgt gcgcctggta aattgcagga ttctcattgt 360

ccggttttct ttatgggaat aatcatcatc accattatca ctgttactct tgcgatcatc 420

atcattaaca taattttttt aacgctgttt gatgatggta tgtgctttta ttgttcctta 480

ctcacctttt cctttgtgtc ttttaatttt gaccattttg accattttga cctttgatga 540

tgtgtgagtt cctcttttct ttttttcttt tcttttttcc tttttttttc ttttcttact 600

gtgttaatca ctttctttcc tttttgttca tattgtcgtc ttgttcattt tcgttcaatt 660

gataatgtat ataaatcttt cgtaagtatc tcttgattgc catttttttc tttccaagtt 720

tccttgttct cgaggccaga aaaaggaagt gtttccctcc ttcttgaatt gatgttaccc 780

tcataaagca cgtggcctct tatcgagaaa gaaattaccg tcgctcgtga tttgtttgca 840

aaaagaacaa aactgaaaaa acccagacac gctcgacttc ctgtcttcct attgattgca 900

gcttccaatt tcgtcacaca acaaggtcct agcgacggct cacaggtttt gtaacaagca 960

atcgaaggtt ctggaatggc gggaaagggt ttagtaccac atgctatgat gcccactgtg 1020

atctccagag caaagttcgt tcgatcgtac tgttactctc tctctttcaa acagaattgt 1080

ccgaatcgtg tgacaacaac agcctgttct cacacactct tttcttctaa ccaagggggt 1140

ggtttagttt agtagaacct cgtgaaactt acatttacat atatataaac ttgcataaat 1200

tggtcaatgc aagaaataca tatttggtct tttctaattc gtagtttttc aagttcttag 1260

atgctttctt tttctctttt ttacagatca tcaaggaagt aattatctac tttttacaag 1320

tctagaatga caacatcaaa tacctacaaa ttctatctaa acggtgaatg gagagaatct 1380

tcctctggag aaactattga gataccatca ccatacttac atgaagtgat cggacaggtt 1440

caagcaatca ctagaggaga ggttgacgaa gcgattgcta gcgctaagga agcacagaaa 1500

tcttgggctg aggcatctct acaagataga gctaagtact tgtacaaatg ggcagatgaa 1560

ttggtaaaca tgcaagacga aatcgccgat atcatcatga aggaagtggg caagggttac 1620

aaagacgcta aaaaggaggt tgttagaacc gccgatttca tcagatacac cattgaagag 1680

gcactccata tgcacggtga atccatgatg ggcgattcat ttcctggtgg aacaaaatct 1740

aagctagcaa taatccaaag agcgcctctg ggtgtagtct tagccatcgc tccattcaat 1800

taccctgtaa acctttctgc tgcaaaattg gcaccagcct taattatggg taacgctgtg 1860

atattcaagc cagcaactca gggtgctatt tccggcatca aaatggttga agctttgcat 1920

aaggctggtt tgccaaaggg tttggttaac gttgccacag gtagaggtag cgtcataggc 1980

gattatttgg tcgaacacga agggataaac atggtttcct tcaccggtgg cactaacact 2040

ggtaagcatt tagcaaaaaa ggcctcaatg attccattag tcttggaact tggtggcaaa 2100

gatccaggca tcgttcgtga agatgcagac ctacaagatg ctgcgaatca tatcgtatct 2160

ggtgcgttca gttactcagg gcagagatgt acagccatta agagagtcct tgttcatgaa 2220

aatgttgctg atgaactggt atcattggtt aaggaacaag tggcaaagct ttctgtggga 2280

tcaccagagc aagattcaac aattgttcct ctgattgacg ataagtccgc tgattttgtt 2340

cagggtttag tggacgatgc agtcgaaaag ggcgctacaa ttgtcattgg gaacaagaga 2400

gaacgtaacc taatctaccc aacattgatt gatcacgtca cagaggaaat gaaagttgcc 2460

tgggaggaac cattcggtcc tattcttcca attattagag ttagtagcga cgagcaagct 2520

attgaaattg caaataagag tgagttcgga ttacaagctt ctgtgtttac caaagacata 2580

aacaaggcat tcgcaatcgc aaataagatt gagactggtt cagtgcaaat caacggtaga 2640

acagagagag gaccagatca ctttcctttt atcggggtta agggatctgg gatgggtgcc 2700

caaggcatca gaaagtcttt ggaatctatg actagagaaa aagttactgt cttaaatctc 2760

gtatgattaa acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt 2820

acattcacgc cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga 2880

agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt 2940

tcaaattttt cttttttttc tgtacaaacg cgtgtacgca tgtaacgggc agacggccgg 3000

ccataacttc gtataatgta tgctatacga agttatcctt acatcacacc caatccccca 3060

caagtgatcc cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag 3120

attttctcgg actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa 3180

atttcccctc tttcttcctc tagggtggcg ttaattaccc gtactaaagg tttggaaaag 3240

aaaaaagaga ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca 3300

cgtttctttt tcttgaaaaa tttttttttt gatttttttc tctttcgatg acctcccatt 3360

gatatttaag ttaataaatg gtcttcaatt tctcaagttt cagtttcgtt tttcttgttc 3420

tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt 3480

tttaattaca aaatgccaca atcctgggaa gaattggccg ccgacaaacg tgcccgtttg 3540

gctaaaacca ttcctgacga atggaaggtt caaactttgc ctgccgaaga ttccgttatt 3600

gatttcccaa agaagtccgg tattttgtct gaggctgaat tgaagattac cgaagcctct 3660

gctgctgatt tggtctccaa gttggccgct ggtgagttga cttctgttga agtcactttg 3720

gctttttgta agagagctgc tattgctcaa caattaacca actgtgctca cgaattcttc 3780

ccagatgctg ctttagctca agctagagaa ttagatgaat actacgctaa gcataagaga 3840

ccagttggtc cattacacgg tttaccaatc tctttaaagg accaattgcg tgttaagggt 3900

tacgaaacct ccatgggtta catttcctgg ttaaacaaat acgatgaagg tgattccgtc 3960

ttaaccacca tgttgagaaa agctggtgct gttttctacg ttaagacctc tgtcccacaa 4020

accttgatgg tctgtgaaac cgtcaacaac atcattggta gaactgtcaa tccaagaaac 4080

aaaaattggt cctgtggtgg ttcttctggt ggtgaaggtg ctattgttgg tattagaggt 4140

ggtgttattg gtgtcggtac tgacattggt ggttccatta gagtcccagc tgctttcaac 4200

tttttatacg gtttgagacc atctcacggt agattgccat atgctaaaat ggctaactct 4260

atggaaggtc aagaaaccgt tcactccgtc gttggtccta tcactcactc cgtcgaagac 4320

ttgagattgt tcaccaaatc tgtcttgggt caagaacctt ggaagtacga ctctaaggtc 4380

atccccatgc catggagaca atctgaatct gacatcattg cctctaagat taagaatggt 4440

ggtttgaaca ttggttatta caatttcgac ggtaacgtct tgccacaccc accaatttta 4500

cgtggtgtcg aaactaccgt tgccgctttg gcggccgcgg ca 4542

<210> 103

<211> 1363

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和构巢曲霉共同组成的序列:

<400> 103

agaggtggtg ttattggtgt cggtactgac attggtggtt ccattagagt cccagctgct 60

ttcaactttt tatacggttt gagaccatct cacggtagat tgccatatgc taaaatggct 120

aactctatgg aaggtcaaga aaccgttcac tccgtcgttg gtcctatcac tcactccgtc 180

gaagacttga gattgttcac caaatctgtc ttgggtcaag aaccttggaa gtacgactct 240

aaggtcatcc ccatgccatg gagacaatct gaatctgaca tcattgcctc taagattaag 300

aatggtggtt tgaacattgg ttattacaat ttcgacggta acgtcttgcc acacccacca 360

attttacgtg gtgtcgaaac taccgttgcc gctttggcca aggctggtca caccgttact 420

ccatggactc catacaagca tgatttctgt catgacttga tttcccacat ctatgctgct 480

gatggttctg ccgacgtcat gagagacatt tctgcctctg gtgagccagc catccctaac 540

attaaggact tgttgaaccc aaatattaag gctgttaaca tgaacgaatt gtgggacact 600

catttacaaa agtggaacta tcaaatggaa tacttggaaa agtggcgtga agctgaagaa 660

aaagctggta aggaattgga cgctattatc gctccaatta ctcctaccgt cgctgtcaga 720

cacgatcaat tcagatacta cggttacgcc tccgttatta gcttattgga tttcacctct 780

gttgtcgtcc cagtcacttt cgctgataag aatattgata agaagaacga atcttttaaa 840

gctgtttccg aattggatgc tttggttcaa gaagaatacg acccagaggc ttatcacggt 900

gctcctgttg ctgttcaagt tattggtaga agattgtccg aagagagaac tttggctatc 960

gccgaagaag tcggtaaatt gttgggtaac gtcgtcactc cataaggaga ttgataagac 1020

ttttctagtt gcatatcttt tatatttaaa tcttatctat tagttaattt tttgtaattt 1080

atccttatat atagtctggt tattctaaaa tatcatttca gtatctaaaa attcccctct 1140

tttttcagtt atatcttaac aggcgataac ttcgtataat gtatgctata cgaagttatg 1200

ataaaactac tacgctaaaa ataaaataaa aatgtatgat ttccctccat ttccgaccaa 1260

ttgtataatt ttatatctgc atgacttaat aatataatat aatacttata aaatacgaat 1320

agaaaaattt aaaccgatgt aatgcatcct tttctttgtt gtc 1363

<210> 104

<211> 5087

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和稻瘟病菌共同组成的序列:

<400> 104

ggccgctcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt 60

ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct 120

gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc 180

ccaggtattg ttagcggttt gaagcaggcg gcggaagaag taacaaagga acctagaggc 240

cttttgatgt tagcagaatt gtcatgcaag ggctccctag ctactggaga atatactaag 300

ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga 360

gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta 420

gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca 480

ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta 540

gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa 600

aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca 660

atttaattat atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa 720

aaaaaaaaaa ttggaaagaa aaagcttcat ggcctttata aaaaggaacc atccaatacc 780

tcgccagaac caagtaacag tattttacgg ggcacaaatc aagaacaata agacaggact 840

gtaaagatgg acgcattgaa ctccaaagaa caacaagagt tccaaaaagt agtggaacaa 900

aagcaaatga aggatttcat gcgtttgata acttcgtata atgtatgcta tacgaagtta 960

tctcgagtaa cacttacaga gtcctatcag gaaagaataa aagccgatca agcttcattc 1020

tcaggtaaca actggagcac tggcgagctt tgctctacta tataagataa aatatgcact 1080

aaaagtttgc atttctttac ataactaaaa ctaagacatt atgcatagct tacctgatca 1140

aaaagtatgt aaacttgtta acatcttcac atgtgattca tctggtcgta ctttcttgcg 1200

gtgcagtgta atatttctac ccacgtgact ataattgagc ttgaaaactg tggcgttttt 1260

ccaccgatgg gtccacgcca gatattaacc gaagccaaaa taccgatgaa atttctgaga 1320

tagctcttgt aaacgacgtc aaatcttcat atgcaaggag atcttgattt ctttttggta 1380

gtcatctgtc gtcttgaggc gtataagaag gaggttatat ctgtcctttc tacaaagtat 1440

tttcgagaat cttgcctctg cccctttttt ctttttttaa aaggtttaaa aaacataact 1500

gtcttcaata tatccagtat ttacgacaat atacaaacat aatctctaga atgaaattca 1560

tctccacctt ccttacattc atcctagcag ccgtgagcgt tacagccgta tacattaacg 1620

gttctataat cacaccatgt gattctttaa tttactgtag aggggaattg ttaaaggaag 1680

tcgaattagc acatccattt gccgatagta agacattcgt tgatatgcca actatcaaac 1740

ctgtcgatga agtgattgaa gccttcaata agttacaaaa gccactgtct aacaacacag 1800

agctacaaga ttttctaaga gagaacttcg cacaagccgg cggagagttg gaagaggttc 1860

caaattctga attagaaacc gatccagttt tcttagacaa attggatgac accgttatca 1920

gagaatttgt tgaaaaagtt attgatatat ggccttcact gacacgtaga tacaagggtc 1980

catctaattg tgaggcttgc gctgactcct ttattccagt taatagaacc ttcgtagttg 2040

ctgggggcag atttagagaa ccatattatt gggacagtta ctggattctt gagggtttgc 2100

tgagaactgg tggcgctttc actaacattt ctaaaaacac cgttgaaaac ttcttagatt 2160

tggtcgaaac aattggcttc gtaccaaacg gtgcacgtat ttactacaaa aatagatcac 2220

aacctccatt gttgtctcaa atggtgcgta tttacgtgga acatacaaat gacaccagta 2280

tcctcggtag agctgtgcct ttgttgatca aggaacatga atttttcatt aacaaccgtt 2340

caatcgatgt tgaggcctcc aacggtaaaa catacagatt acagagatac gctgtaacca 2400

atactcaacc aagacctgag tcttatagag aagattacat aacagccagt aacagatcct 2460

actactcacc atctggtatc atctaccctg aatcccacca actaaacgaa tctgaaaagg 2520

ctgttctgta tagccattta gcatcagggg cagaatctgg atgggactat acctcaagat 2580

ggctctctac accttctgat gccgtgagag ataactactt tccattgaga tcactaaata 2640

caaacaatat agttccagta gaccttaact caattttgta cgcaaacgaa gtggccatcg 2700

ctgagttcct taacagaact ggaaacagta caggtgcatc agattggatg gatctagcaa 2760

agcaaagatc agaggctatg tacgccctta tgtggaatga aactctctgg tcatactttg 2820

attacaacat gacatctaag actcaaaaca gattcatacc agtcgatgaa gatgcagttt 2880

caattgagac aaataacgct ccagcaggac aacaagtctt tttccatgtc gcacaatact 2940

accctttctg gactggcgca gccccaagat ccttgaaaaa taatccattg gctgttttga 3000

gagcatacga gcgtatcgat gcttacttgg acatcaagcg tggagcaatt ccagcgacaa 3060

acttaaagac tggtcagcaa tgggacgagc caaacgtctg gcctccatta atgcacattc 3120

tgatggaagg gctgaccaga gtaccagcga catttgggga agatgatgtc gcatggacag 3180

aaatacaaga cttagctcta agacttggtc agagatactt agattctact ttttgcactt 3240

ggtacgctac tggtggttca acatctgaaa caccacagct acagggattg aacgctgaag 3300

ataagggtat catgttcgaa aagtacggtg acaattctac aaacgttgct ggttccggcg 3360

gggagtacga agtggttgag ggtttcggtt ggacaaatgg tgttttgatg tgggtcgcag 3420

atactttcaa taacaagctc actagacctg attgcggtaa cattaccgct gcaaatgttc 3480

actccgacgg ttctcaagcc agaaagagag gtgagatgtg gtcagcttta gaaatgcatc 3540

cttacgatgc agcttggact aaagagttcg gtgcgagaaa ggttagaaga gacaaggccg 3600

aagcgagagc acttggaaat gttatgggcg gcgtctgatt aattaattta ccagcttact 3660

atccttcttg aaaatatgca ctctatatct tttagttctt aattgcaaca catagatttg 3720

ctgtataacg aattttatgc tattttttta atttggagtt cggtgatgaa agtgtcacag 3780

cgaatttcct cacatgtagg gaccgaattg tttacaagtt ctctgtacca ccatggagac 3840

atcaaagatt gaaaatctat ggaaagatat ggacggtagc aacaagaata tagcacgagc 3900

cgcggagttc atttcgttac ttttgatatc gctcacaact attgcgaagc gcttcagtga 3960

aaaaatcata aggaaaagtt gtaaatatta ttggtagtat tcgtttggta aagtagaggg 4020

ggtaattttt cccctttatt ttgttcatac attcttaaat tgctttgcct ctccttttgg 4080

aaagctatac ttcggagcac tgttgagcga aggctcaggc cggcagcacg cagcacgctg 4140

tatttacgta tttaatttta tatatttgtg catacactac tagggaagac ttgaaaaaaa 4200

cctaggaaat gaaaaaacga cacaggaagt cccgtattta ctattttttc cttccttttg 4260

atggggcagg gcggaaatag aggataggat aagcctactg cttagctgtt tccgtctcta 4320

cttcggtagt tgtctcaatt gtcgtttcag tattaccttt agagccgcta gacgatggtt 4380

gagctatttg ttgagggaaa actaagttca tgtaacacac gcataacccg attaaactca 4440

tgaatagctt gattgcagga ggctggtcca ttggagatgg tgccttattt tccttatagg 4500

caacgatgat gtcttcgtcg gtgttcaggt agtagtgtac actctgaatc agggagaacc 4560

aggcaatgaa cttgttcctc aagaaaatag cggccatagg catggattgg ttaaccacac 4620

cagatatgct tggtgtggca gaatatagtc cttttggtgg cgcaattttc ttgtacctgt 4680

ggtagaaagg gagcggttga actgttagta tatattggca atatcagcaa atttgaaaga 4740

aaattgtcgg tgaaaaacat acgaaacaca aaggtcgggc cttgcaacgt tattcaaagt 4800

cattgtttag ttgaggaggt agcagcggag tatatgtatt cctttttttt gcctatggat 4860

gttgtaccat gcccattctg ctcaagcttt tgttaaaatt atttttcagt attttttctt 4920

ccatgttgcg cgttacgaga acagaagcga cagataaccg caatcataca actagcgcta 4980

ctgcggggtg taaaaagcac aagaactaag ccaagatcac aacagttatc gataaaatag 5040

cagtgtttgc atggccattg agaaggacaa cattggcgtg cggcatg 5087

<210> 105

<211> 5236

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母、稻瘟病菌和构巢曲霉共同组成的序列:

<400> 105

ggccgcgaag gtgctattgt tggtattaga ggtggtgtta ttggtgtcgg tactgacatt 60

ggtggttcca ttagagtccc agctgctttc aactttttat acggtttgag accatctcac 120

ggtagattgc catatgctaa aatggctaac tctatggaag gtcaagaaac cgttcactcc 180

gtcgttggtc ctatcactca ctccgtcgaa gacttgagat tgttcaccaa atctgtcttg 240

ggtcaagaac cttggaagta cgactctaag gtcatcccaa tgccatggag acaatctgaa 300

tctgacatca ttgcctctaa gattaagaat ggtggtttga acattggtta ttacaatttc 360

gacggtaacg tcttgccaca cccaccaatt ttacgtggtg tcgaaactac cgttgccgct 420

ttggccaagg ctggtcacac cgttactcca tggactccat acaagcatga tttcggtcat 480

gacttgattt cccacatcta tgctgctgat ggttctgccg acgtcatgag agacatttct 540

gcctctggtg agccagccat ccctaacatt aaggacttgt tgaacccaaa tattaaggct 600

gttaacatga acgaattgtg ggacactcat ttacaaaagt ggaactatca aatggaatac 660

ttggaaaagt ggcgtgaagc tgaagaaaaa gctggtaagg aattggacgc tattatcgct 720

ccaattactc ctaccgccgc tgtcagacac gatcaattca gatactacgg ttacgcctcc 780

gttattaact tattggattt cacctctgtt gtcgtcccag tcactttcgc tgataagaat 840

attgataaga agaacgaatc ttttaaagct gtttccgaat tggatgcttt ggttcaagaa 900

gaatacgacc cagaggctta tcacggtgct cctgttgctg ttcaagttat tggtagaaga 960

ttgtccgaag agagaacttt ggctatcgcc gaagaagtcg gtaaattgtt gggtaacgtc 1020

gtcactccat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa 1080

aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc 1140

ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt 1200

attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcacc 1260

caattgtaga tatgctaact ccagcaatga gttgatgaat ctcggtgtgt attttatgtc 1320

ctcagaggac aacacataac ttcgtataat gtatgctata cgaagttatc tcgagtaaca 1380

cttacagagt cctatcagga aagaataaaa gccgatcaag cttcattctc aggtaacaac 1440

tggagcactg gcgagctttg ctctactata taagataaaa tatgcactaa aagtttgcat 1500

ttctttacat aactaaaact aagacattat gcatagctta cctgatcaaa aagtatgtaa 1560

acttgttaac atcttcacat gtgattcatc tggtcgtact ttcttgcggt gcagtgtaat 1620

atttctaccc acgtgactat aattgagctt gaaaactgtg gcgtttttcc accgatgggt 1680

ccacgccaga tattaaccga agccaaaata ccgatgaaat ttctgagata gctcttgtaa 1740

acgacgtcaa atcttcatat gcaaggagat cttgatttct ttttggtagt catctgtcgt 1800

cttgaggcgt ataagaagga ggttatatct gtcctttcta caaagtattt tcgagaatct 1860

tgcctctgcc ccttttttct ttttttaaaa ggtttaaaaa acataactgt cttcaatata 1920

tccagtattt acgacaatat acaaacataa tctctagaat gaaattcatc tccaccttcc 1980

ttacattcat cctagcagcc gtgagcgtta cagccgtata cattaacggt tctataatca 2040

caccatgtga ttctttaatt tactgtagag gggaattgtt aaaggaagtc gaattagcac 2100

atccatttgc cgatagtaag acattcgttg atatgccaac tatcaaacct gtcgatgaag 2160

tgattgaagc cttcaataag ttacaaaagc cactgtctaa caacacagag ctacaagatt 2220

ttctaagaga gaacttcgca caagccggcg gagagttgga agaggttcca aattctgaat 2280

tagaaaccga tccagttttc ttagacaaat tggatgacac cgttatcaga gaatttgttg 2340

aaaaagttat tgatatatgg ccttcactga cacgtagata caagggtcca tctaattgtg 2400

aggcttgcgc tgactccttt attccagtta atagaacctt cgtagttgct gggggcagat 2460

ttagagaacc atattattgg gacagttact ggattcttga gggtttgctg agaactggtg 2520

gcgctttcac taacatttct aaaaacaccg ttgaaaactt cttagatttg gtcgaaacaa 2580

ttggcttcgt accaaacggt gcacgtattt actacaaaaa tagatcacaa cctccattgt 2640

tgtctcaaat ggtgcgtatt tacgtggaac atacaaatga caccagtatc ctcggtagag 2700

ctgtgccttt gttgatcaag gaacatgaat ttttcattaa caaccgttca atcgatgttg 2760

aggcctccaa cggtaaaaca tacagattac agagatacgc tgtaaccaat actcaaccaa 2820

gacctgagtc ttatagagaa gattacataa cagccagtaa cagatcctac tactcaccat 2880

ctggtatcat ctaccctgaa tcccaccaac taaacgaatc tgaaaaggct gttctgtata 2940

gccatttagc atcaggggca gaatctggat gggactatac ctcaagatgg ctctctacac 3000

cttctgatgc cgtgagagat aactactttc cattgagatc actaaataca aacaatatag 3060

ttccagtaga ccttaactca attttgtacg caaacgaagt ggccatcgct gagttcctta 3120

acagaactgg aaacagtaca ggtgcatcag attggatgga tctagcaaag caaagatcag 3180

aggctatgta cgcccttatg tggaatgaaa ctctctggtc atactttgat tacaacatga 3240

catctaagac tcaaaacaga ttcataccag tcgatgaaga tgcagtttca attgagacaa 3300

ataacgctcc agcaggacaa caagtctttt tccatgtcgc acaatactac cctttctgga 3360

ctggcgcagc cccaagatcc ttgaaaaata atccattggc tgttttgaga gcatacgagc 3420

gtatcgatgc ttacttggac atcaagcgtg gagcaattcc agcgacaaac ttaaagactg 3480

gtcagcaatg ggacgagcca aacgtctggc ctccattaat gcacattctg atggaagggc 3540

tgaccagagt accagcgaca tttggggaag atgatgtcgc atggacagaa atacaagact 3600

tagctctaag acttggtcag agatacttag attctacttt ttgcacttgg tacgctactg 3660

gtggttcaac atctgaaaca ccacagctac agggattgaa cgctgaagat aagggtatca 3720

tgttcgaaaa gtacggtgac aattctacaa acgttgctgg ttccggcggg gagtacgaag 3780

tggttgaggg tttcggttgg acaaatggtg ttttgatgtg ggtcgcagat actttcaata 3840

acaagctcac tagacctgat tgcggtaaca ttaccgctgc aaatgttcac tccgacggtt 3900

ctcaagccag aaagagaggt gagatgtggt cagctttaga aatgcatcct tacgatgcag 3960

cttggactaa agagttcggt gcgagaaagg ttagaagaga caaggccgaa gcgagagcac 4020

ttggaaatgt tatgggcggc gtctgattaa ttaatttacc agcttactat ccttcttgaa 4080

aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct gtataacgaa 4140

ttttatgcta tttttttaat ttggagttcg gtgatgaaag tgtcacagcg aatttcctca 4200

catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat caaagattga 4260

aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg cggagttcat 4320

ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa aaatcataag 4380

gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg taatttttcc 4440

cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa agctatactt 4500

cggagcactg ttgagcgaag gctcaggccg gcagcacgca gcacgctgta tttacgtatt 4560

taattttata tatttgtgca tacactacta gggaagactt gaaaaaaacc taggaaatga 4620

aaaaacgaca caggaagtcc cgtatttact attttttcct tccttttgat ggggcagggc 4680

ggaaatagag gataggataa gcctactgct tagctgtttc cgtctctact tcggtagttg 4740

tctcaattgt cgtttcagta ttacctttag agccgctaga cgatggttga gctatttgtt 4800

gagggaaaac taagttcatg taacacacgc ataacccgat taaactcatg aatagcttga 4860

ttgcaggagg ctggtccatt ggagatggtg ccttattttc cttataggca acgatgatgt 4920

cttcgtcggt gttcaggtag tagtgtacac tctgaatcag ggagaaccag gcaatgaact 4980

tgttcctcaa gaaaatagcg gccataggca tggattggtt aaccacacca gatatgcttg 5040

gtgtggcaga atatagtcct tttggtggcg caattttctt gtacctgtgg tagaaaggga 5100

gcggttgaac tgttagtata tattggcaat atcagcaaat ttgaaagaaa attgtcggtg 5160

aaaaacatac gaaacacaaa ggtcgggcct tgcaacgtta ttcaaagtca ttgtttagtt 5220

gaggaggtag cagcgg 5236

<210> 106

<211> 6386

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母和光滑假丝酵母共同组成的序列:

<400> 106

ggccgctcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt 60

ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct 120

gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc 180

ccaggtattg ttagcggttt gaagcaggcg gcggaagaag taacaaagga acctagaggc 240

cttttgatgt tagcagaatt gtcatgcaag ggctccctag ctactggaga atatactaag 300

ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga 360

gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta 420

gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca 480

ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta 540

gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa 600

aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca 660

atttaattat atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa 720

aaaaaaaaaa ttggaaagaa aaagcttcat ggcctttata aaaaggaacc atccaatacc 780

tcgccagaac caagtaacag tattttacgg ggcacaaatc aagaacaata agacaggact 840

gtaaagatgg acgcattgaa ctccaaagaa caacaagagt tccaaaaagt agtggaacaa 900

aagcaaatga aggatttcat gcgtttgata acttcgtata atgtatgcta tacgaagtta 960

tctcgagtaa cacttacaga gtcctatcag gaaagaataa aagccgatca agcttcattc 1020

tcaggtaaca actggagcac tggcgagctt tgctctacta tataagataa aatatgcact 1080

aaaagtttgc atttctttac ataactaaaa ctaagacatt atgcatagct tacctgatca 1140

aaaagtatgt aaacttgtta acatcttcac atgtgattca tctggtcgta ctttcttgcg 1200

gtgcagtgta atatttctac ccacgtgact ataattgagc ttgaaaactg tggcgttttt 1260

ccaccgatgg gtccacgcca gatattaacc gaagccaaaa taccgatgaa atttctgaga 1320

tagctcttgt aaacgacgtc aaatcttcat atgcaaggag atcttgattt ctttttggta 1380

gtcatctgtc gtcttgaggc gtataagaag gaggttatat ctgtcctttc tacaaagtat 1440

tttcgagaat cttgcctctg cccctttttt ctttttttaa aaggtttaaa aaacataact 1500

gtcttcaata tatccagtat ttacgacaat atacaaacat aatctctaga atgaaattca 1560

tctctacttt ccttactttt attttagctg ccgtatctgt aaccgcctct accaactctt 1620

acttctcttc ctccttagct tctgcttcac cattattcaa caaagagggc agagtggtta 1680

gaccaatgcg tgaaagtaac ttaggcctac acgcagatcc tcaaactaga aaatccagta 1740

agacacttta cgatttgttg tcagactttg acaatgcatt ttacgatgat gaaaacatga 1800

tcttaggatc cctagccttc ggtgagaata cctactccag acagccatac gtcgcaaacg 1860

ggtacatagg tagccgtata ccaaacatcg gtttcggata cgcacttgat acacttaact 1920

tgtacgctga tgcaccaggc gcacttaaca acggttggcc tttgagaaac agaagattcg 1980

ctggaagttt cgtttctgat ttctactcac tgcaagccaa gttgaacagt accaattttc 2040

ctgagttaga tgaaaagggt tatacaactg tcatttcatc aatcccagaa tggacagatt 2100

tgcaattcac tgtagacctt aacggcacta actggttcaa tccacaatca gttctaatcg 2160

atgatgttat taattacaat cagaacttga gcatgaagga tggaattgtt agcacaaaca 2220

tggattggtt gaacggaatg ataaacatta agtctgaagt ctgggcacac agaaagatcc 2280

attccttggg tatcacacgt ctggaaatct cattgaacct tgatgcttta cctgacgagt 2340

tcactgaact accagttaca atttacgata tcatagattt caacacttct catagaacaa 2400

cactatatga gaaaggtcaa gatgaagata acaaagcgat ttacatgatt gtaaacccag 2460

aaaacgtacc atactcaaac gcagttgtgt actctacttg tacaatcaag ggcactgaaa 2520

acaacttttc tccttacaac ttcacatcag atgatagaat tgcgagaaac tacatgacaa 2580

acctaacaga agagaaccca aaagtcgtta tttacaagta cacatcagtg gtttcctcag 2640

aatataataa tgacgagcca aatcctaacg ttaacttgaa gttcgcctca aacatcgcta 2700

acaccgcaaa aggaaactat aagtctttat tgtccaatca caaaagagct tggtacgatt 2760

tgtacaatga tgcctttatc gaaattccat ctgatagttt attggaaatg accgcaagat 2820

ctagtctctt ccatttgctt gcgaacacac gtcaatacaa cgtttctaca actagaggat 2880

taccagtcgg cgttggtggt ctttcttcag actcttacgg ggggatggtc ttttgggacg 2940

cagacgtatg gatggctcca gctctcctac ctttctttcc aaacatagca atgaacatga 3000

ataactacag aaacgctact catcaacaag ccattgaaaa tgctaagcaa tacaattacc 3060

caggcgccgt ttacccttgg acatctggta gatatgcgaa ttgtacctct accggtcctt 3120

gcatcgacta cgaataccat attaatgtag atatcgcttt ggcaagtttc tctatttaca 3180

tgaacggtgc cgagggtgcc gacgaagatt accttagatt cacaacatgg ccaatggtca 3240

aggatgctgc agtgttcttc aaggcctacg tcaaatacaa tgaaacactt ggagagtacg 3300

aaacttataa tttaactgac ccagatgaat ttgctaatca tgtgaataat ggagctttca 3360

caaatgcagg aatcaaaact ttgttgaaat gggcaacaga cattggtact caccttgggg 3420

aggaagttga tcctaagtgg atggaaattg ctgataacat acatattcct agaagtgact 3480

ctaacattac tttggagtac tctggcatga atagctccgt tgagattaag caggctgacg 3540

ttacattgat ggtataccct ttaggttaca tcaacgatga atctatacta aacaacgcaa 3600

tcaaggattt gtattactac tctgaacgtc agtctgcctc tgggccagct atgacttacc 3660

cagtgttcgt cgcagctgcc gcttccctct tgaaccacgg ttcatcatca cagagttacc 3720

tttacaaatc tgttcttcca tatctgagat caccatttgc tcaatttagc gaacaatcag 3780

atgacaattt cttaactaac ggtttgacac aaccagcatt cccatttttg accgctaatg 3840

gtggtttctt gcaatctata ttatttggat taaccggatt gagatacagt tacgaagtta 3900

ctcctagaac caaaaagatt tcaagactac tcaagtttga tccagtcaag ttgccactgc 3960

tacctggcgg aattgcaatt agaaatttca aatacatggg acaagtactg gatatcataa 4020

tagacgataa caatggtaca atagctcata agggtgggga taagccaatt agaatcaagg 4080

ttccaaatag agatattctc cacgacagaa atatcacttc cgcactatac tcaaagagag 4140

atgacgattt atcagcgaca gatgactact acggtacata cttcacacta tacccaaatg 4200

aggaactggt gattcctctt tacgatacta agttaaacat cgacggtaac atagccgaat 4260

ctaaacaaat aactaatttg actgccggag ttccaggtga cgttggtttc tccgcattag 4320

atgggaacaa ttacacacac tggcagccat tcgataaatc agataatgct aagttgctaa 4380

ttgacttagg ttttaacagc actcatgtca ttaaaaaggg tattattttg tggggtcaaa 4440

gaccagcgaa aaacatttct ttgtcagttt tgccacattc agagagaatt gagcaactat 4500

ttgcgaacat cacagatctt ttagaaacat catctataac taagggcggc tctttgttaa 4560

accagatgtt aggccaaaca caatcaaacg tgaccgcaga aattgatgat gatatcctcg 4620

ctttactgaa ctggaaaggt gacgatctgg accaactcat cccttatttg cctgatatgc 4680

atctcctaca agagaaattc attcctattt tgaaggatta ccctattaag ccaaatcaga 4740

gatactataa ggaaatcatt gacgatgaca taatcaagtt attaccatca aacacaactg 4800

aatttacaat cgattacaat tctatccctg gcggggaaaa gcgtgcaaga tacgttgttc 4860

tgacagttca cggcacctat gatgacgatg atgatcttaa aggtgcaaca atcaaggaaa 4920

ttgttctgca ggaatgatta attaatttac cagcttacta tccttcttga aaatatgcac 4980

tctatatctt ttagttctta attgcaacac atagatttgc tgtataacga attttatgct 5040

atttttttaa tttggagttc ggtgatgaaa gtgtcacagc gaatttcctc acatgtaggg 5100

accgaattgt ttacaagttc tctgtaccac catggagaca tcaaagattg aaaatctatg 5160

gaaagatatg gacggtagca acaagaatat agcacgagcc gcggagttca tttcgttact 5220

tttgatatcg ctcacaacta ttgcgaagcg cttcagtgaa aaaatcataa ggaaaagttg 5280

taaatattat tggtagtatt cgtttggtaa agtagagggg gtaatttttc ccctttattt 5340

tgttcataca ttcttaaatt gctttgcctc tccttttgga aagctatact tcggagcact 5400

gttgagcgaa ggctcaggcc ggcagcacgc agcacgctgt atttacgtat ttaattttat 5460

atatttgtgc atacactact agggaagact tgaaaaaaac ctaggaaatg aaaaaacgac 5520

acaggaagtc ccgtatttac tattttttcc ttccttttga tggggcaggg cggaaataga 5580

ggataggata agcctactgc ttagctgttt ccgtctctac ttcggtagtt gtctcaattg 5640

tcgtttcagt attaccttta gagccgctag acgatggttg agctatttgt tgagggaaaa 5700

ctaagttcat gtaacacacg cataacccga ttaaactcat gaatagcttg attgcaggag 5760

gctggtccat tggagatggt gccttatttt ccttataggc aacgatgatg tcttcgtcgg 5820

tgttcaggta gtagtgtaca ctctgaatca gggagaacca ggcaatgaac ttgttcctca 5880

agaaaatagc ggccataggc atggattggt taaccacacc agatatgctt ggtgtggcag 5940

aatatagtcc ttttggtggc gcaattttct tgtacctgtg gtagaaaggg agcggttgaa 6000

ctgttagtat atattggcaa tatcagcaaa tttgaaagaa aattgtcggt gaaaaacata 6060

cgaaacacaa aggtcgggcc ttgcaacgtt attcaaagtc attgtttagt tgaggaggta 6120

gcagcggagt atatgtattc cttttttttg cctatggatg ttgtaccatg cccattctgc 6180

tcaagctttt gttaaaatta tttttcagta ttttttcttc catgttgcgc gttacgagaa 6240

cagaagcgac agataaccgc aatcatacaa ctagcgctac tgcggggtgt aaaaagcaca 6300

agaactaagc caagatcaca acagttatcg ataaaatagc agtgtttgca tggccattga 6360

gaaggacaac attggcgtgc ggcatg 6386

<210> 107

<211> 6535

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 由多种生物体酿酒酵母、光滑假丝酵母和构巢曲霉共同组成的序列:

<400> 107

ggccgcgaag gtgctattgt tggtattaga ggtggtgtta ttggtgtcgg tactgacatt 60

ggtggttcca ttagagtccc agctgctttc aactttttat acggtttgag accatctcac 120

ggtagattgc catatgctaa aatggctaac tctatggaag gtcaagaaac cgttcactcc 180

gtcgttggtc ctatcactca ctccgtcgaa gacttgagat tgttcaccaa atctgtcttg 240

ggtcaagaac cttggaagta cgactctaag gtcatcccaa tgccatggag acaatctgaa 300

tctgacatca ttgcctctaa gattaagaat ggtggtttga acattggtta ttacaatttc 360

gacggtaacg tcttgccaca cccaccaatt ttacgtggtg tcgaaactac cgttgccgct 420

ttggccaagg ctggtcacac cgttactcca tggactccat acaagcatga tttcggtcat 480

gacttgattt cccacatcta tgctgctgat ggttctgccg acgtcatgag agacatttct 540

gcctctggtg agccagccat ccctaacatt aaggacttgt tgaacccaaa tattaaggct 600

gttaacatga acgaattgtg ggacactcat ttacaaaagt ggaactatca aatggaatac 660

ttggaaaagt ggcgtgaagc tgaagaaaaa gctggtaagg aattggacgc tattatcgct 720

ccaattactc ctaccgccgc tgtcagacac gatcaattca gatactacgg ttacgcctcc 780

gttattaact tattggattt cacctctgtt gtcgtcccag tcactttcgc tgataagaat 840

attgataaga agaacgaatc ttttaaagct gtttccgaat tggatgcttt ggttcaagaa 900

gaatacgacc cagaggctta tcacggtgct cctgttgctg ttcaagttat tggtagaaga 960

ttgtccgaag agagaacttt ggctatcgcc gaagaagtcg gtaaattgtt gggtaacgtc 1020

gtcactccat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa 1080

aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc 1140

ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt 1200

attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcacc 1260

caattgtaga tatgctaact ccagcaatga gttgatgaat ctcggtgtgt attttatgtc 1320

ctcagaggac aacacataac ttcgtataat gtatgctata cgaagttatc tcgagtaaca 1380

cttacagagt cctatcagga aagaataaaa gccgatcaag cttcattctc aggtaacaac 1440

tggagcactg gcgagctttg ctctactata taagataaaa tatgcactaa aagtttgcat 1500

ttctttacat aactaaaact aagacattat gcatagctta cctgatcaaa aagtatgtaa 1560

acttgttaac atcttcacat gtgattcatc tggtcgtact ttcttgcggt gcagtgtaat 1620

atttctaccc acgtgactat aattgagctt gaaaactgtg gcgtttttcc accgatgggt 1680

ccacgccaga tattaaccga agccaaaata ccgatgaaat ttctgagata gctcttgtaa 1740

acgacgtcaa atcttcatat gcaaggagat cttgatttct ttttggtagt catctgtcgt 1800

cttgaggcgt ataagaagga ggttatatct gtcctttcta caaagtattt tcgagaatct 1860

tgcctctgcc ccttttttct ttttttaaaa ggtttaaaaa acataactgt cttcaatata 1920

tccagtattt acgacaatat acaaacataa tctctagaat gaaattcatc tctactttcc 1980

ttacttttat tttagctgcc gtatctgtaa ccgcctctac caactcttac ttctcttcct 2040

ccttagcttc tgcttcacca ttattcaaca aagagggcag agtggttaga ccaatgcgtg 2100

aaagtaactt aggcctacac gcagatcctc aaactagaaa atccagtaag acactttacg 2160

atttgttgtc agactttgac aatgcatttt acgatgatga aaacatgatc ttaggatccc 2220

tagccttcgg tgagaatacc tactccagac agccatacgt cgcaaacggg tacataggta 2280

gccgtatacc aaacatcggt ttcggatacg cacttgatac acttaacttg tacgctgatg 2340

caccaggcgc acttaacaac ggttggcctt tgagaaacag aagattcgct ggaagtttcg 2400

tttctgattt ctactcactg caagccaagt tgaacagtac caattttcct gagttagatg 2460

aaaagggtta tacaactgtc atttcatcaa tcccagaatg gacagatttg caattcactg 2520

tagaccttaa cggcactaac tggttcaatc cacaatcagt tctaatcgat gatgttatta 2580

attacaatca gaacttgagc atgaaggatg gaattgttag cacaaacatg gattggttga 2640

acggaatgat aaacattaag tctgaagtct gggcacacag aaagatccat tccttgggta 2700

tcacacgtct ggaaatctca ttgaaccttg atgctttacc tgacgagttc actgaactac 2760

cagttacaat ttacgatatc atagatttca acacttctca tagaacaaca ctatatgaga 2820

aaggtcaaga tgaagataac aaagcgattt acatgattgt aaacccagaa aacgtaccat 2880

actcaaacgc agttgtgtac tctacttgta caatcaaggg cactgaaaac aacttttctc 2940

cttacaactt cacatcagat gatagaattg cgagaaacta catgacaaac ctaacagaag 3000

agaacccaaa agtcgttatt tacaagtaca catcagtggt ttcctcagaa tataataatg 3060

acgagccaaa tcctaacgtt aacttgaagt tcgcctcaaa catcgctaac accgcaaaag 3120

gaaactataa gtctttattg tccaatcaca aaagagcttg gtacgatttg tacaatgatg 3180

cctttatcga aattccatct gatagtttat tggaaatgac cgcaagatct agtctcttcc 3240

atttgcttgc gaacacacgt caatacaacg tttctacaac tagaggatta ccagtcggcg 3300

ttggtggtct ttcttcagac tcttacgggg ggatggtctt ttgggacgca gacgtatgga 3360

tggctccagc tctcctacct ttctttccaa acatagcaat gaacatgaat aactacagaa 3420

acgctactca tcaacaagcc attgaaaatg ctaagcaata caattaccca ggcgccgttt 3480

acccttggac atctggtaga tatgcgaatt gtacctctac cggtccttgc atcgactacg 3540

aataccatat taatgtagat atcgctttgg caagtttctc tatttacatg aacggtgccg 3600

agggtgccga cgaagattac cttagattca caacatggcc aatggtcaag gatgctgcag 3660

tgttcttcaa ggcctacgtc aaatacaatg aaacacttgg agagtacgaa acttataatt 3720

taactgaccc agatgaattt gctaatcatg tgaataatgg agctttcaca aatgcaggaa 3780

tcaaaacttt gttgaaatgg gcaacagaca ttggtactca ccttggggag gaagttgatc 3840

ctaagtggat ggaaattgct gataacatac atattcctag aagtgactct aacattactt 3900

tggagtactc tggcatgaat agctccgttg agattaagca ggctgacgtt acattgatgg 3960

tatacccttt aggttacatc aacgatgaat ctatactaaa caacgcaatc aaggatttgt 4020

attactactc tgaacgtcag tctgcctctg ggccagctat gacttaccca gtgttcgtcg 4080

cagctgccgc ttccctcttg aaccacggtt catcatcaca gagttacctt tacaaatctg 4140

ttcttccata tctgagatca ccatttgctc aatttagcga acaatcagat gacaatttct 4200

taactaacgg tttgacacaa ccagcattcc catttttgac cgctaatggt ggtttcttgc 4260

aatctatatt atttggatta accggattga gatacagtta cgaagttact cctagaacca 4320

aaaagatttc aagactactc aagtttgatc cagtcaagtt gccactgcta cctggcggaa 4380

ttgcaattag aaatttcaaa tacatgggac aagtactgga tatcataata gacgataaca 4440

atggtacaat agctcataag ggtggggata agccaattag aatcaaggtt ccaaatagag 4500

atattctcca cgacagaaat atcacttccg cactatactc aaagagagat gacgatttat 4560

cagcgacaga tgactactac ggtacatact tcacactata cccaaatgag gaactggtga 4620

ttcctcttta cgatactaag ttaaacatcg acggtaacat agccgaatct aaacaaataa 4680

ctaatttgac tgccggagtt ccaggtgacg ttggtttctc cgcattagat gggaacaatt 4740

acacacactg gcagccattc gataaatcag ataatgctaa gttgctaatt gacttaggtt 4800

ttaacagcac tcatgtcatt aaaaagggta ttattttgtg gggtcaaaga ccagcgaaaa 4860

acatttcttt gtcagttttg ccacattcag agagaattga gcaactattt gcgaacatca 4920

cagatctttt agaaacatca tctataacta agggcggctc tttgttaaac cagatgttag 4980

gccaaacaca atcaaacgtg accgcagaaa ttgatgatga tatcctcgct ttactgaact 5040

ggaaaggtga cgatctggac caactcatcc cttatttgcc tgatatgcat ctcctacaag 5100

agaaattcat tcctattttg aaggattacc ctattaagcc aaatcagaga tactataagg 5160

aaatcattga cgatgacata atcaagttat taccatcaaa cacaactgaa tttacaatcg 5220

attacaattc tatccctggc ggggaaaagc gtgcaagata cgttgttctg acagttcacg 5280

gcacctatga tgacgatgat gatcttaaag gtgcaacaat caaggaaatt gttctgcagg 5340

aatgattaat taatttacca gcttactatc cttcttgaaa atatgcactc tatatctttt 5400

agttcttaat tgcaacacat agatttgctg tataacgaat tttatgctat ttttttaatt 5460

tggagttcgg tgatgaaagt gtcacagcga atttcctcac atgtagggac cgaattgttt 5520

acaagttctc tgtaccacca tggagacatc aaagattgaa aatctatgga aagatatgga 5580

cggtagcaac aagaatatag cacgagccgc ggagttcatt tcgttacttt tgatatcgct 5640

cacaactatt gcgaagcgct tcagtgaaaa aatcataagg aaaagttgta aatattattg 5700

gtagtattcg tttggtaaag tagagggggt aatttttccc ctttattttg ttcatacatt 5760

cttaaattgc tttgcctctc cttttggaaa gctatacttc ggagcactgt tgagcgaagg 5820

ctcaggccgg cagcacgcag cacgctgtat ttacgtattt aattttatat atttgtgcat 5880

acactactag ggaagacttg aaaaaaacct aggaaatgaa aaaacgacac aggaagtccc 5940

gtatttacta ttttttcctt ccttttgatg gggcagggcg gaaatagagg ataggataag 6000

cctactgctt agctgtttcc gtctctactt cggtagttgt ctcaattgtc gtttcagtat 6060

tacctttaga gccgctagac gatggttgag ctatttgttg agggaaaact aagttcatgt 6120

aacacacgca taacccgatt aaactcatga atagcttgat tgcaggaggc tggtccattg 6180

gagatggtgc cttattttcc ttataggcaa cgatgatgtc ttcgtcggtg ttcaggtagt 6240

agtgtacact ctgaatcagg gagaaccagg caatgaactt gttcctcaag aaaatagcgg 6300

ccataggcat ggattggtta accacaccag atatgcttgg tgtggcagaa tatagtcctt 6360

ttggtggcgc aattttcttg tacctgtggt agaaagggag cggttgaact gttagtatat 6420

attggcaata tcagcaaatt tgaaagaaaa ttgtcggtga aaaacatacg aaacacaaag 6480

gtcgggcctt gcaacgttat tcaaagtcat tgtttagttg aggaggtagc agcgg 6535

<210> 108

<211> 605

<212> PRT

<213> 小孢根霉(Rhizopus microsporus)

<400> 108

Met Lys Leu Met Asn Pro Ser Met Lys Ala Tyr Val Phe Phe Ile Leu

1 5 10 15

Ser Tyr Phe Ser Leu Leu Val Ser Ser Ala Ala Val Pro Thr Ser Ala

20 25 30

Ala Val Gln Val Glu Ser Tyr Asn Tyr Asp Gly Thr Thr Phe Ser Gly

35 40 45

Arg Ile Phe Val Lys Asn Ile Ala Tyr Ser Lys Val Val Thr Val Ile

50 55 60

Tyr Ser Asp Gly Ser Asp Asn Trp Asn Asn Asn Asn Asn Lys Val Ser

65 70 75 80

Ala Ala Tyr Ser Glu Ala Ile Ser Gly Ser Asn Tyr Glu Tyr Trp Thr

85 90 95

Phe Ser Ala Lys Leu Ser Gly Ile Lys Gln Phe Tyr Val Lys Tyr Glu

100 105 110

Val Ser Gly Ser Thr Tyr Tyr Asp Asn Asn Gly Thr Lys Asn Tyr Gln

115 120 125

Val Gln Ala Thr Ser Ala Thr Ser Thr Thr Ala Thr Ala Thr Thr Thr

130 135 140

Thr Ala Thr Gly Thr Thr Thr Thr Ser Thr Gly Pro Thr Ser Thr Ala

145 150 155 160

Ser Val Ser Phe Pro Thr Gly Asn Ser Thr Ile Ser Ser Trp Ile Lys

165 170 175

Asn Gln Glu Glu Ile Ser Arg Phe Ala Met Leu Arg Asn Ile Asn Pro

180 185 190

Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser Thr Ala Gly

195 200 205

Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu Thr Ala Asn

210 215 220

Val Ile Ala Tyr Glu Tyr Asn Thr Thr Phe Thr Gly Asn Thr Thr Leu

225 230 235 240

Leu Lys Tyr Leu Lys Asp Tyr Val Thr Phe Ser Val Lys Ser Gln Ser

245 250 255

Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Ala Asp

260 265 270

Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro

275 280 285

Ala Glu Arg Ala Val Thr Phe Met Leu Ile Ala Asp Ser Tyr Leu Thr

290 295 300

Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys Pro Ala Ile

305 310 315 320

Phe Lys Asp Leu Asp Tyr Val Val Ser Val Trp Ser Asn Gly Cys Tyr

325 330 335

Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Val

340 345 350

Met Arg Lys Gly Leu Ile Leu Gly Ala Asp Phe Ala Ala Arg Asn Gly

355 360 365

Asp Ser Ser Arg Ala Ser Thr Tyr Lys Gln Thr Ala Ser Thr Met Glu

370 375 380

Ser Lys Ile Ser Ser Phe Trp Ser Asp Ser Asn Asn Tyr Val Gln Val

385 390 395 400

Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys Gly Leu Asp Val Ser

405 410 415

Thr Leu Leu Ala Ala Asn Ile Gly Ser Leu Pro Asp Gly Phe Phe Thr

420 425 430

Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Leu Glu Asn Ala

435 440 445

Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu Pro Ser Tyr Leu Gly

450 455 460

Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn

465 470 475 480

Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn Ala Tyr Ala Glu Leu

485 490 495

Tyr Tyr Arg Ala Ile Lys Glu Trp Ile Ser Asn Gly Lys Val Thr Val

500 505 510

Ser Asn Ile Ser Leu Pro Phe Phe Lys Lys Phe Asp Ser Ser Ala Thr

515 520 525

Ser Gly Lys Thr Tyr Thr Ala Gly Thr Ser Asp Phe Asn Asn Leu Ala

530 535 540

Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu Ser Thr Val Lys Phe

545 550 555 560

His Ala Tyr Thr Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Ser Thr

565 570 575

Gly Met Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu

580 585 590

Ile Thr Val Ala Tyr Ala Lys Ala Gly Ser Pro Ala Ala

595 600 605

<210> 109

<211> 604

<212> PRT

<213> 戴尔根霉(Rhizopus delemar)

<400> 109

Met Gln Leu Phe Asn Leu Pro Leu Lys Val Ser Phe Phe Leu Val Leu

1 5 10 15

Ser Tyr Phe Ser Leu Leu Val Ser Ala Ala Ser Ile Pro Ser Ser Ala

20 25 30

Ser Val Gln Leu Asp Ser Tyr Asn Tyr Asp Gly Ser Thr Phe Ser Gly

35 40 45

Lys Ile Tyr Val Lys Asn Ile Ala Tyr Ser Lys Lys Val Thr Val Ile

50 55 60

Tyr Ala Asp Gly Ser Asp Asn Trp Asn Asn Asn Gly Asn Thr Ile Ala

65 70 75 80

Ala Ser Tyr Ser Ala Pro Ile Ser Gly Ser Asn Tyr Glu Tyr Trp Thr

85 90 95

Phe Ser Ala Ser Ile Asn Gly Ile Lys Glu Phe Tyr Ile Lys Tyr Glu

100 105 110

Val Ser Gly Lys Thr Tyr Tyr Asp Asn Asn Asn Ser Ala Asn Tyr Gln

115 120 125

Val Ser Thr Ser Lys Pro Thr Thr Thr Thr Ala Thr Ala Thr Thr Thr

130 135 140

Thr Ala Pro Ser Thr Ser Thr Thr Thr Pro Pro Ser Ser Ser Glu Pro

145 150 155 160

Ala Thr Phe Pro Thr Gly Asn Ser Thr Ile Ser Ser Trp Ile Lys Lys

165 170 175

Gln Glu Gly Ile Ser Arg Phe Ala Met Leu Arg Asn Ile Asn Pro Pro

180 185 190

Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser Thr Ala Gly Pro

195 200 205

Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ala Ala Leu Thr Ser Asn Val

210 215 220

Ile Val Tyr Glu Tyr Asn Thr Thr Leu Ser Gly Asn Lys Thr Ile Leu

225 230 235 240

Asn Val Leu Lys Asp Tyr Val Thr Phe Ser Val Lys Thr Gln Ser Thr

245 250 255

Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly

260 265 270

Ser Gly Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala

275 280 285

Glu Arg Ala Thr Thr Phe Ile Leu Phe Ala Asp Ser Tyr Leu Thr Gln

290 295 300

Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys Pro Ala Ile Phe

305 310 315 320

Lys Asp Leu Asp Tyr Val Val Asn Val Trp Ser Asn Gly Cys Phe Asp

325 330 335

Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Val Met

340 345 350

Arg Lys Gly Leu Leu Leu Gly Ala Asp Phe Ala Lys Arg Asn Gly Asp

355 360 365

Ser Thr Arg Ala Ser Thr Tyr Ser Ser Thr Ala Ser Thr Ile Ala Asn

370 375 380

Lys Ile Ser Ser Phe Trp Val Ser Ser Asn Asn Trp Ile Gln Val Ser

385 390 395 400

Gln Ser Val Thr Gly Gly Val Ser Lys Lys Gly Leu Asp Val Ser Thr

405 410 415

Leu Leu Ala Ala Asn Leu Gly Ser Val Asp Asp Gly Phe Phe Thr Pro

420 425 430

Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Val Glu Asp Ser Phe

435 440 445

Ala Ser Leu Tyr Pro Ile Asn Lys Asn Leu Pro Ser Tyr Leu Gly Asn

450 455 460

Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser

465 470 475 480

Gln Gly Asn Pro Trp Phe Leu Ala Val Thr Gly Tyr Ala Glu Leu Tyr

485 490 495

Tyr Arg Ala Ile Lys Glu Trp Ile Gly Asn Gly Gly Val Thr Val Ser

500 505 510

Ser Ile Ser Leu Pro Phe Phe Lys Lys Phe Asp Ser Ser Ala Thr Ser

515 520 525

Gly Lys Lys Tyr Thr Val Gly Thr Ser Asp Phe Asn Asn Leu Ala Gln

530 535 540

Asn Ile Ala Leu Ala Ala Asp Arg Phe Leu Ser Thr Val Gln Leu His

545 550 555 560

Ala His Asn Asn Gly Ser Leu Ala Glu Glu Phe Asp Arg Thr Thr Gly

565 570 575

Leu Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu Ile

580 585 590

Thr Ala Ser Tyr Ala Lys Ala Gly Ala Pro Ala Ala

595 600

<210> 110

<211> 1440

<212> DNA

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 110

atgacaacat caaataccta caaattctat ctaaacggtg aatggagaga atcttcctct 60

ggagaaacta ttgagatacc atcaccatac ttacatgaag tgatcggaca ggttcaagca 120

atcactagag gagaggttga cgaagcgatt gctagcgcta aggaagcaca gaaatcttgg 180

gctgaggcat ctctacaaga tagagctaag tacttgtaca aatgggcaga tgaattggta 240

aacatgcaag acgaaatcgc cgatatcatc atgaaggaag tgggcaaggg ttacaaagac 300

gctaaaaagg aggttgttag aaccgccgat ttcatcagat acaccattga agaggcactc 360

catatgcacg gtgaatccat gatgggcgat tcatttcctg gtggaacaaa atctaagcta 420

gcaataatcc aaagagcgcc tctgggtgta gtcttagcca tcgctccatt caattaccct 480

gtaaaccttt ctgctgcaaa attggcacca gccttaatta tgggtaacgc tgtgatattc 540

aagccagcaa ctcagggtgc tatttccggc atcaaaatgg ttgaagcttt gcataaggct 600

ggtttgccaa agggtttggt taacgttgcc acaggtagag gtagcgtcat aggcgattat 660

ttggtcgaac acgaagggat aaacatggtt tccttcaccg gtggcactaa cactggtaag 720

catttagcaa aaaaggcctc aatgattcca ttagtcttgg aacttggtgg caaagatcca 780

ggcatcgttc gtgaagatgc agacctacaa gatgctgcga atcatatcgt atctggtgcg 840

ttcagttact cagggcagag atgtacagcc attaagagag tccttgttca tgaaaatgtt 900

gctgatgaac tggtatcatt ggttaaggaa caagtggcaa agctttctgt gggatcacca 960

gagcaagatt caacaattgt tcctctgatt gacgataagt ccgctgattt tgttcagggt 1020

ttagtggacg atgcagtcga aaagggcgct acaattgtca ttgggaacaa gagagaacgt 1080

aacctaatct acccaacatt gattgatcac gtcacagagg aaatgaaagt tgcctgggag 1140

gaaccattcg gtcctattct tccaattatt agagttagta gcgacgagca agctattgaa 1200

attgcaaata agagtgagtt cggattacaa gcttctgtgt ttaccaaaga cataaacaag 1260

gcattcgcaa tcgcaaataa gattgagact ggttcagtgc aaatcaacgg tagaacagag 1320

agaggaccag atcactttcc ttttatcggg gttaagggat ctgggatggg tgcccaaggc 1380

atcagaaagt ctttggaatc tatgactaga gaaaaagtta ctgtcttaaa tctcgtatga 1440

<210> 111

<211> 479

<212> PRT

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 111

Met Thr Thr Ser Asn Thr Tyr Lys Phe Tyr Leu Asn Gly Glu Trp Arg

1 5 10 15

Glu Ser Ser Ser Gly Glu Thr Ile Glu Ile Pro Ser Pro Tyr Leu His

20 25 30

Glu Val Ile Gly Gln Val Gln Ala Ile Thr Arg Gly Glu Val Asp Glu

35 40 45

Ala Ile Ala Ser Ala Lys Glu Ala Gln Lys Ser Trp Ala Glu Ala Ser

50 55 60

Leu Gln Asp Arg Ala Lys Tyr Leu Tyr Lys Trp Ala Asp Glu Leu Val

65 70 75 80

Asn Met Gln Asp Glu Ile Ala Asp Ile Ile Met Lys Glu Val Gly Lys

85 90 95

Gly Tyr Lys Asp Ala Lys Lys Glu Val Val Arg Thr Ala Asp Phe Ile

100 105 110

Arg Tyr Thr Ile Glu Glu Ala Leu His Met His Gly Glu Ser Met Met

115 120 125

Gly Asp Ser Phe Pro Gly Gly Thr Lys Ser Lys Leu Ala Ile Ile Gln

130 135 140

Arg Ala Pro Leu Gly Val Val Leu Ala Ile Ala Pro Phe Asn Tyr Pro

145 150 155 160

Val Asn Leu Ser Ala Ala Lys Leu Ala Pro Ala Leu Ile Met Gly Asn

165 170 175

Ala Val Ile Phe Lys Pro Ala Thr Gln Gly Ala Ile Ser Gly Ile Lys

180 185 190

Met Val Glu Ala Leu His Lys Ala Gly Leu Pro Lys Gly Leu Val Asn

195 200 205

Val Ala Thr Gly Arg Gly Ser Val Ile Gly Asp Tyr Leu Val Glu His

210 215 220

Glu Gly Ile Asn Met Val Ser Phe Thr Gly Gly Thr Asn Thr Gly Lys

225 230 235 240

His Leu Ala Lys Lys Ala Ser Met Ile Pro Leu Val Leu Glu Leu Gly

245 250 255

Gly Lys Asp Pro Gly Ile Val Arg Glu Asp Ala Asp Leu Gln Asp Ala

260 265 270

Ala Asn His Ile Val Ser Gly Ala Phe Ser Tyr Ser Gly Gln Arg Cys

275 280 285

Thr Ala Ile Lys Arg Val Leu Val His Glu Asn Val Ala Asp Glu Leu

290 295 300

Val Ser Leu Val Lys Glu Gln Val Ala Lys Leu Ser Val Gly Ser Pro

305 310 315 320

Glu Gln Asp Ser Thr Ile Val Pro Leu Ile Asp Asp Lys Ser Ala Asp

325 330 335

Phe Val Gln Gly Leu Val Asp Asp Ala Val Glu Lys Gly Ala Thr Ile

340 345 350

Val Ile Gly Asn Lys Arg Glu Arg Asn Leu Ile Tyr Pro Thr Leu Ile

355 360 365

Asp His Val Thr Glu Glu Met Lys Val Ala Trp Glu Glu Pro Phe Gly

370 375 380

Pro Ile Leu Pro Ile Ile Arg Val Ser Ser Asp Glu Gln Ala Ile Glu

385 390 395 400

Ile Ala Asn Lys Ser Glu Phe Gly Leu Gln Ala Ser Val Phe Thr Lys

405 410 415

Asp Ile Asn Lys Ala Phe Ala Ile Ala Asn Lys Ile Glu Thr Gly Ser

420 425 430

Val Gln Ile Asn Gly Arg Thr Glu Arg Gly Pro Asp His Phe Pro Phe

435 440 445

Ile Gly Val Lys Gly Ser Gly Met Gly Ala Gln Gly Ile Arg Lys Ser

450 455 460

Leu Glu Ser Met Thr Arg Glu Lys Val Thr Val Leu Asn Leu Val

465 470 475

<210> 112

<211> 577

<212> DNA

<213> 酿酒酵母(Saccharomyces cerevisiae)

<400> 112

taacacttac agagtcctat caggaaagaa taaaagccga tcaagcttca ttctcaggta 60

acaactggag cactggcgag ctttgctcta ctatataaga taaaatatgc actaaaagtt 120

tgcatttctt tacataacta aaactaagac attatgcata gcttacctga tcaaaaagta 180

tgtaaacttg ttaacatctt cacatgtgat tcatctggtc gtactttctt gcggtgcagt 240

gtaatatttc tacccacgtg actataattg agcttgaaaa ctgtggcgtt tttccaccga 300

tgggtccacg ccagatatta accgaagcca aaataccgat gaaatttctg agatagctct 360

tgtaaacgac gtcaaatctt catatgcaag gagatcttga tttctttttg gtagtcatct 420

gtcgtcttga ggcgtataag aaggaggtta tatctgtcct ttctacaaag tattttcgag 480

aatcttgcct ctgccccttt tttctttttt taaaaggttt aaaaaacata actgtcttca 540

atatatccag tatttacgac aatatacaaa cataatc 577

Claims

1.一种经遗传修饰的酵母，其包含

编码具有以下多肽序列的海藻糖酶多肽的异源基因，所述多肽序列为：SEQ ID NO：1、SEQ ID NO：2、SEQ ID NO：3、SEQ ID NO：87、SEQ ID NO：8、SEQ ID NO：11、SEQ ID NO：14或SEQ ID NO：92；

减少或消除的一种或多种编码甘油-3-磷酸磷酸酶的基因的表达；以及

编码葡糖淀粉酶多肽的异源基因，

其中当所述酵母存在于包含海藻糖的发酵培养基中时，所述酵母能够产生乙醇，并且所述酵母以足以在所述乙醇滴度为至少110g/L时将发酵液的海藻糖含量降低至小于2g/L的量分泌海藻糖酶。

2.如权利要求1所述的酵母，其中由所述酵母编码的所述海藻糖酶多肽包含SEQ IDNO：83序列。

3.如权利要求1-2中任一项所述的酵母，其中由所述酵母编码的所述海藻糖酶多肽包含SEQ ID NO：84和/或SEQ ID NO：85序列。

4.如权利要求1-2中任一项所述的酵母，其中所述酵母是经遗传修饰的酿酒酵母。

5.如权利要求1-2中任一项所述的酵母，其中由所述酵母编码的所述海藻糖酶包含MFα2信号序列。

6.如权利要求5所述的酵母，其中所述MFα2信号序列是SEQ ID NO：4。

7.如权利要求1-2中任一项所述的酵母，其中所述酵母以足以在所述乙醇滴度为至少为110g/L时，将发酵液中的海藻糖含量降低至小于0.5g/L或1g/L的量分泌海藻糖酶。

8.如权利要求1-2中任一项所述的酵母，其中所述酵母能够在细胞外分泌所述海藻糖酶。

9.如权利要求1-2中任一项所述的酵母，其中由所述酵母编码的所述海藻糖酶多肽包含SEQ ID NO：86序列。

10.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于80g/L的滴度产生乙醇。

11.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于90g/L的滴度产生乙醇。

12.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于100g/L的滴度产生乙醇。

13.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于110g/L的滴度产生乙醇。

14.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于120g/L的滴度产生乙醇。

15.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于125g/L的滴度产生乙醇。

16.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于130g/L的滴度产生乙醇。

17.如权利要求1-2中任一项所述的酵母，其中所述酵母能够以高于135g/L的滴度产生乙醇。

18.一种用于生产乙醇的方法，所述方法包括：

使用经遗传修饰的酵母使培养基发酵，其中所述酵母包含

编码具有以下多肽序列的海藻糖酶多肽的异源海藻糖酶基因，所述多肽序列为：SEQID NO：1、SEQ ID NO：2、SEQ ID NO：3、SEQ ID NO：87、SEQ ID NO：8、SEQ ID NO：11、SEQ IDNO：14或SEQ ID NO：92；

编码葡糖淀粉酶多肽的异源基因，

其中如接种后36小时所测量的，所述乙醇滴度为至少105g/L，并且发酵结束时所述发酵液的所述海藻糖含量小于2g/L。

19.如权利要求18所述的方法，其中所述酵母是如权利要求1-17中任一项所述的酵母。