BR112018004377A2 - cepas de levedura para a expressão e secreção de proteínas heterólogas em temperaturas elevadas - Google Patents

cepas de levedura para a expressão e secreção de proteínas heterólogas em temperaturas elevadas

Info

Publication number
BR112018004377A2
BR112018004377A2 BR112018004377A BR112018004377A BR112018004377A2 BR 112018004377 A2 BR112018004377 A2 BR 112018004377A2 BR 112018004377 A BR112018004377 A BR 112018004377A BR 112018004377 A BR112018004377 A BR 112018004377A BR 112018004377 A2 BR112018004377 A2 BR 112018004377A2
Authority
BR
Brazil
Prior art keywords
yeast strains
secretion
expression
elevated temperatures
heterologous proteins
Prior art date
Application number
BR112018004377A
Other languages
English (en)
Inventor
Argyros Aaron
F Rice Charles
Skinner Ryan
Barrett Trisha
Original Assignee
Lallemand Hungary Liquidity Man Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Man Llc filed Critical Lallemand Hungary Liquidity Man Llc
Publication of BR112018004377A2 publication Critical patent/BR112018004377A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente divulgação refere-se a cepas de levedura recombinantes capazes de manter a sua robustez em temperatura elevada, bem como proteínas recombinantes expressas a partir das mesmas. a presente divulgação também fornece métodos para usar a cepa de levedura recombinante para produzir um produto de fermentação. a presente divulgação apresenta ainda um processo para produzir cepas de levedura recombinantes capazes de manter sua robustez em temperatura elevada.
BR112018004377A 2015-09-04 2016-08-29 cepas de levedura para a expressão e secreção de proteínas heterólogas em temperaturas elevadas BR112018004377A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562214412P 2015-09-04 2015-09-04
US201662299897P 2016-02-25 2016-02-25
PCT/IB2016/055162 WO2017037614A1 (en) 2015-09-04 2016-08-29 Yeast strains for the expression and secretion of heterologous proteins at high temperatures

Publications (1)

Publication Number Publication Date
BR112018004377A2 true BR112018004377A2 (pt) 2019-04-09

Family

ID=56896745

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018004377A BR112018004377A2 (pt) 2015-09-04 2016-08-29 cepas de levedura para a expressão e secreção de proteínas heterólogas em temperaturas elevadas

Country Status (7)

Country Link
US (1) US11332728B2 (pt)
EP (1) EP3344762A1 (pt)
CN (1) CN108291213A (pt)
BR (1) BR112018004377A2 (pt)
CA (1) CA2997380A1 (pt)
MX (1) MX2018002705A (pt)
WO (1) WO2017037614A1 (pt)

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US20220090102A1 (en) 2016-12-22 2022-03-24 Lallemand Hungary Liquidity Management Llc Sulfite tolerance in recombinant yeast host cells
EP3596208A1 (en) * 2017-03-13 2020-01-22 Lallemand Hungary Liquidity Management LLC Recombinant yeast host cells expressing cell-associated heterologous proteins
BR122022015725B1 (pt) 2017-05-23 2023-03-14 Lallemand Hungary Liquidity Management Llc Processo para a produção de um produto de fermentação
WO2019175809A1 (en) * 2018-03-13 2019-09-19 Lallemand Hungary Liquidity Management Llc Inactivated yeast and yeast product for improving fermentation yield
CN112601818A (zh) 2018-07-25 2021-04-02 诺维信公司 用于生产乙醇的表达酶的酵母
BR112021006692A2 (pt) 2018-10-08 2021-08-10 Novozymes A/S levedura expressando enzimas para produção de etanol
WO2020089847A1 (en) 2018-11-02 2020-05-07 Lallemand Hungary Liquidity Management Llc Process for preventing or limiting microbial contamination during continuous culture
US20220220487A1 (en) 2018-11-13 2022-07-14 Lallemand Hungary Liquidity Management Llc Combined expression of trehalose producing and trehalose degrading enzymes
BR112021009163A2 (pt) 2018-11-13 2021-08-17 Lallemand Hungary Liquidity Management Llc. combinações sinergísticas de bactéria e levedura
WO2020100069A1 (en) 2018-11-13 2020-05-22 Lallemand Hungary Liquidity Management Llc Modulation of formate oxidation by recombinant yeast host cell during fermentation
CA3121603A1 (en) 2018-12-07 2020-06-11 Lallemand Hungary Liquidity Management Llc Modulation of nadph generation by recombinant yeast host cell during fermentation
EP4010469A1 (en) 2019-08-06 2022-06-15 Novozymes A/S Fusion proteins for improved enzyme expression
BR112022010438A2 (pt) * 2019-11-29 2022-10-11 Lallemand Hungary Liquidity Man Llc Processo para deslocamento de uma enzima exógena
WO2021104672A1 (en) 2019-11-29 2021-06-03 Lallemand Hungary Liquidity Management Llc Yeast expressing heterologous glucoamylase
WO2021119304A1 (en) 2019-12-10 2021-06-17 Novozymes A/S Microorganism for improved pentose fermentation
BR112022015805A2 (pt) 2020-02-10 2022-10-11 Novozymes As Variante de alfa-amilase de uma alfa-amilase genitora, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, composição, formulação de caldo inteiro ou composição de cultura de células, uso de uma alfa-amilase variante, processo de produção de um produto de fermentação a partir de material contendo amido e de um produto de xarope, e, planta transgênica, parte de planta ou célula de planta
WO2021163015A1 (en) 2020-02-10 2021-08-19 Novozymes A/S Process for producing ethanol from raw starch using alpha-amylase variants
BR102021005223A2 (pt) 2020-03-19 2021-10-19 Lallemand Hungary Liquidity Management Llc. Coculturas bacterianas expressando um sistema de bacteriocina
BR102021005224A2 (pt) 2020-03-19 2021-09-28 Lallemand Hungary Liquidity Management Llc. Levedura que expressa glucoamilase com hidrólise reforçada de amido
BR112022022797A2 (pt) 2020-05-11 2022-12-13 Lallemand Hungary Liquidity Man Llc Célula hospedeira de levedura recombinante que expressa uma hidrolase
CN112831485B (zh) * 2021-01-13 2023-08-15 云南师范大学 一种低温活性改良的外切菊粉酶突变体MutDR121EH9
US20240084244A1 (en) 2021-01-26 2024-03-14 Danstar Ferment Ag Recombinant yeast host cell having enhanced growth rate
CN112662575B (zh) * 2021-01-28 2022-10-21 武汉轻工大学 高蛋白酶活性及高出酒率的扣囊复膜酵母菌、其组合物及应用
CA3222371A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation
WO2023062542A2 (en) 2021-10-11 2023-04-20 Lallemand Hungary Liquidity Management Llc Recombinant yeast cell having increased pyruvate decarboxylase activity
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast

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JP4460876B2 (ja) * 2003-11-07 2010-05-12 株式会社豊田中央研究所 有機酸存在下におけるプロモーター及びその利用
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BRPI0817005A2 (pt) * 2007-09-18 2019-09-24 Basf Plant Science Gmbh método para produzir uma célula de planta transgênica, uma planta ou uma parte da mesma com rendimento aumentado, molécula de ácido nucleico, construção de ácido nucleico, vetor, célula hospedeira, processo para produzir um polipeptídeo, polipeptídeo, anticorpo, núcleo de célula de planta transgênica, célula de planta, tecido de planta, material de propagação, material colhido, planta ou parte da mesma, semente, processo para identificar um composto, método para produzir uma composição agrícola, composição, e, uso de uma molécula de ácido nucleico
EP2260105B1 (en) * 2008-02-29 2016-08-17 The Trustees Of The University Of Pennsylvania Production and use of plant degrading materials
CN103060216B (zh) 2008-05-06 2016-07-06 阿彻丹尼尔斯米德兰德公司 通过淀粉和木聚糖的降解酶的表达开发能进行淀粉和木聚糖的醇发酵的耐热酵母多形汉逊酵母菌株
EP2519626A4 (en) * 2009-12-30 2013-05-22 Iogen Energy Corp MODIFIED YEAST STRAINS HAVING INCREASED FERMENTATION OF LIGNOCELLULOSIC HYDROLYSATES
CA2801577A1 (en) * 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
CN103649321B (zh) 2011-04-05 2019-07-16 拉勒曼德匈牙利流动性管理有限责任公司 用于通过添加交替电子受体改善微生物中的产品收率和产量的方法
JP5878396B2 (ja) * 2012-03-02 2016-03-08 トヨタ自動車株式会社 新規プロモーター及びその利用
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Also Published As

Publication number Publication date
EP3344762A1 (en) 2018-07-11
US11332728B2 (en) 2022-05-17
WO2017037614A1 (en) 2017-03-09
CN108291213A (zh) 2018-07-17
US20180265853A1 (en) 2018-09-20
CA2997380A1 (en) 2017-03-09
MX2018002705A (es) 2018-12-19

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]