BR112022010438A2 - Processo para deslocamento de uma enzima exógena - Google Patents

Processo para deslocamento de uma enzima exógena

Info

Publication number
BR112022010438A2
BR112022010438A2 BR112022010438A BR112022010438A BR112022010438A2 BR 112022010438 A2 BR112022010438 A2 BR 112022010438A2 BR 112022010438 A BR112022010438 A BR 112022010438A BR 112022010438 A BR112022010438 A BR 112022010438A BR 112022010438 A2 BR112022010438 A2 BR 112022010438A2
Authority
BR
Brazil
Prior art keywords
starch
heterologous polypeptide
displacement
exogenous enzyme
dextrin
Prior art date
Application number
BR112022010438A
Other languages
English (en)
Inventor
Argyros Aaron
Panaitiu Alexandra
F Rice Charles
E Barrett Kenneth
B Richards Matthew
Stoughtenger Tera
Original Assignee
Lallemand Hungary Liquidity Man Llc
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Man Llc, Basf Se filed Critical Lallemand Hungary Liquidity Man Llc
Publication of BR112022010438A2 publication Critical patent/BR112022010438A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCESSO PARA DESLOCAMENTO DE UMA ENZIMA EXÓGENA. A presente invenção refere-se a um processo para fermentar uma biomassa com uma dose reduzida de uma enzima exógena purificada (que pode ser, por exemplo, uma glucoamilase exógena purificada). O processo compreende colocar em contato uma biomassa (que pode compreender amido) com uma célula hospedeira de levedura recombinante. A célula hospedeira de levedura recombinante tem uma modificação genética para expressar um polipeptídeo heterólogo com atividade de amido ou dextrina hidrolase (que pode ser, por exemplo, de uma glucoamilase). A molécula de ácido nucleico que codifica o polipeptídeo heterólogo com atividade de amido ou dextrina hidrolase compreende permitir a secreção do polipeptídeo heterólogo.
BR112022010438A 2019-11-29 2020-11-25 Processo para deslocamento de uma enzima exógena BR112022010438A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962941967P 2019-11-29 2019-11-29
US202063078139P 2020-09-14 2020-09-14
US202063078135P 2020-09-14 2020-09-14
PCT/EP2020/025539 WO2021104673A1 (en) 2019-11-29 2020-11-25 Process for displacing an exogenous enzyme

Publications (1)

Publication Number Publication Date
BR112022010438A2 true BR112022010438A2 (pt) 2022-10-11

Family

ID=73740356

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022010438A BR112022010438A2 (pt) 2019-11-29 2020-11-25 Processo para deslocamento de uma enzima exógena

Country Status (4)

Country Link
US (1) US20210163995A1 (pt)
BR (1) BR112022010438A2 (pt)
CA (1) CA3163131A1 (pt)
WO (1) WO2021104673A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9605269B2 (en) 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
WO2012138942A1 (en) 2011-04-05 2012-10-11 Mascoma Corporation Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
EP3344762A1 (en) * 2015-09-04 2018-07-11 Lallemand Hungary Liquidity Management LLC Yeast strains for the expression and secretion of heterologous proteins at high temperatures
US10570421B2 (en) 2015-11-06 2020-02-25 Lallemand Hungary Liquidity Management Llc Limiting yeast-produced trehalose in fermentation
CA3029161A1 (en) * 2016-07-01 2018-01-04 Lallemand Hungary Liquidity Management Llc Alpha-amylases for combination with glucoamylases for improving saccharification
EP3494129A1 (en) * 2016-08-05 2019-06-12 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
IL269303B1 (en) 2017-03-13 2024-04-01 Lallemand Hungary Liquidity Man Llc Recombinant yeast cells expressing heterologous cell-associated proteins
WO2020100069A1 (en) 2018-11-13 2020-05-22 Lallemand Hungary Liquidity Management Llc Modulation of formate oxidation by recombinant yeast host cell during fermentation

Also Published As

Publication number Publication date
CA3163131A1 (en) 2021-06-03
US20210163995A1 (en) 2021-06-03
WO2021104673A1 (en) 2021-06-03

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