BR112016021857A2 - via alterada de célula hospedeira para produção de etanol aprimorada - Google Patents

via alterada de célula hospedeira para produção de etanol aprimorada

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Publication number
BR112016021857A2
BR112016021857A2 BR112016021857A BR112016021857A BR112016021857A2 BR 112016021857 A2 BR112016021857 A2 BR 112016021857A2 BR 112016021857 A BR112016021857 A BR 112016021857A BR 112016021857 A BR112016021857 A BR 112016021857A BR 112016021857 A2 BR112016021857 A2 BR 112016021857A2
Authority
BR
Brazil
Prior art keywords
cell
host cell
ethanol production
activity
cell pathway
Prior art date
Application number
BR112016021857A
Other languages
English (en)
Other versions
BR112016021857B1 (pt
Inventor
Miasnikov Andrei
W Munos Jeffrey
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of BR112016021857A2 publication Critical patent/BR112016021857A2/pt
Publication of BR112016021857B1 publication Critical patent/BR112016021857B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1018—Carboxy- and carbamoyl transferases (2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/02—Aldehyde-lyases (4.1.2)
    • C12Y401/02009—Phosphoketolase (4.1.2.9)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

a presente invenção refere-se a uma célula de levedura recombinante, a composições de fermentação, e aos métodos de uso das mesmas. as células de levedura recombinantes incluem ao menos um ácido nucleico heterólogo que codifica um ou mais polipeptídeos com atividade de fosfocetolase; atividade fosfotransacetilase; e/ou atividade de acetilação de acetaldeído desidrogenase, em que a célula não inclui um gene de xilose redutase modificado heterólogo, e em que a célula tem a capacidade de produção aumentada de produto bioquímico final em um processo de fermentação quando comparada à célula de levedura parental.
BR112016021857-4A 2014-03-28 2015-03-19 Célula de levedura recombinante e método para produzir etanol a partir de glicose BR112016021857B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461971745P 2014-03-28 2014-03-28
US61/971,745 2014-03-28
PCT/US2015/021558 WO2015148272A1 (en) 2014-03-28 2015-03-19 Altered host cell pathway for improved ethanol production

Publications (2)

Publication Number Publication Date
BR112016021857A2 true BR112016021857A2 (pt) 2017-10-24
BR112016021857B1 BR112016021857B1 (pt) 2024-03-12

Family

ID=52824564

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016021857-4A BR112016021857B1 (pt) 2014-03-28 2015-03-19 Célula de levedura recombinante e método para produzir etanol a partir de glicose

Country Status (6)

Country Link
US (2) US10240168B2 (pt)
EP (1) EP3122876B1 (pt)
CN (1) CN106687576B (pt)
BR (1) BR112016021857B1 (pt)
DK (1) DK3122876T3 (pt)
WO (1) WO2015148272A1 (pt)

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ES2767728T3 (es) * 2015-10-06 2020-06-18 Dsm Ip Assets Bv Célula eucariota con elevada producción de producto de fermentación
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BR112019011093A2 (pt) 2016-12-20 2019-10-22 Dsm Ip Assets Bv cepas de levedura aperfeiçoadas para a produção de etanol
MX2019009124A (es) * 2017-02-02 2019-09-27 Lallemand Hungary Liquidity Man Llc Expresion de proteasa heterologa para mejorar la fermentacion alcoholica.
US20200024619A1 (en) 2017-03-21 2020-01-23 Dsm Ip Assets B.V. Improved glycerol free ethanol production
BR112019019701A2 (pt) * 2017-03-24 2020-07-14 Danisco Us Inc. redução de acetato e glicerol em levedura modificada que tem um caminho de produção de etanol exógeno
BR112019021553A2 (pt) 2017-04-17 2020-05-12 Danisco Us Inc. Enzimas de acetil-coa sintase selecionadas para redução de produção de acetato em levedura
WO2018204798A1 (en) 2017-05-04 2018-11-08 Cargill, Incorporated Genetically modified trehalase-expressing yeasts and fermentation processes using such genetically modified yeasts
US20200157581A1 (en) * 2017-06-02 2020-05-21 Novozymes A/S Improved Yeast For Ethanol Production
CN110741014B (zh) * 2017-06-06 2024-04-09 丹尼斯科美国公司 具有改善的醇生产的酵母
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WO2019173225A1 (en) 2018-03-06 2019-09-12 Danisco Us Inc Yeast with improved alcohol production under high dissolved solids conditions
BR112020018055A2 (pt) 2018-03-06 2020-12-29 Danisco Us Inc. Redução na produção de acetato por meio de levedura que superexpressa pab1
US20210032642A1 (en) 2018-04-06 2021-02-04 Danisco Us Inc. Increased alcohol production from yeast producing an increased amount of active hac1 protein
UY38179A (es) 2018-04-12 2019-11-29 Danisco Us Inc Levadura que sobreexpresa proteína fosfatasas asociadas con la vía hog
BR112020023951A2 (pt) * 2018-05-25 2021-02-23 Danisco Us Inc. resultados de superexpressão de fumarato redutase em uma taxa de fermentação aumentada em levedura
EP3802784A1 (en) 2018-05-30 2021-04-14 Danisco US Inc. Over-expression of transcriptional activator/repressor gis1 in yeast for increased ethanol production
EP3830282A1 (en) * 2018-07-27 2021-06-09 Danisco US Inc. Increased alcohol production from yeast producing an increased amount of active crz1 protein
CN109182301B (zh) * 2018-08-17 2021-12-07 广东溢多利生物科技股份有限公司 一种二糖降解酶基因及其应用
US20210332091A1 (en) 2018-09-28 2021-10-28 Danisco Us Inc Over-expression of gds1 in yeast for increased ethanol and decreased acetate production
US20210395756A1 (en) 2018-09-28 2021-12-23 Danisco Us Inc Over expression of ribonucleotide reductase inhibitor in yeast for increased ethanol production
WO2020068907A1 (en) 2018-09-28 2020-04-02 Danisco Us Inc Selected phosphotransacetylase genes for increased ethanol production in engineered yeast
EP3938381A1 (en) 2019-03-14 2022-01-19 Danisco US Inc. Over-expression of cytochrome b2 in yeast for increased ethanol production
EP3938519A1 (en) 2019-03-14 2022-01-19 Danisco US Inc. Over-expression of fumarate-succinate transporter in yeast for increased ethanol and reduced acetate production
CN114302958A (zh) 2019-06-24 2022-04-08 丹尼斯科美国公司 用于增加发酵联产物中赖氨酸含量的经修饰的酵母和方法
US20220251608A1 (en) 2019-06-24 2022-08-11 Danisco Us Inc Disruption of cdc42 effectors in yeast for increased alcohol and lysine production
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Also Published As

Publication number Publication date
CN106687576A (zh) 2017-05-17
US20190127765A1 (en) 2019-05-02
WO2015148272A1 (en) 2015-10-01
CN106687576B (zh) 2021-10-29
US10428354B2 (en) 2019-10-01
EP3122876B1 (en) 2020-11-25
US20170088861A1 (en) 2017-03-30
EP3122876A1 (en) 2017-02-01
DK3122876T3 (da) 2021-02-08
US10240168B2 (en) 2019-03-26
BR112016021857B1 (pt) 2024-03-12

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