EA201390667A1 - Ферменты и их применения - Google Patents

Ферменты и их применения

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Publication number
EA201390667A1
EA201390667A1 EA201390667A EA201390667A EA201390667A1 EA 201390667 A1 EA201390667 A1 EA 201390667A1 EA 201390667 A EA201390667 A EA 201390667A EA 201390667 A EA201390667 A EA 201390667A EA 201390667 A1 EA201390667 A1 EA 201390667A1
Authority
EA
Eurasian Patent Office
Prior art keywords
enzymes
biomass
relates
field
methods
Prior art date
Application number
EA201390667A
Other languages
English (en)
Inventor
Жан-Поль Леонетти
Жан-Мишель Клавери
Паскаль Жозеф
Люси Ру
Original Assignee
Деинов
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Деинов filed Critical Деинов
Publication of EA201390667A1 publication Critical patent/EA201390667A1/ru

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
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    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
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    • C12Y301/01072—Acetylxylan esterase (3.1.1.72)
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
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    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Paper (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Изобретение относится к новым ферментам и их применениям. Изобретение также относится к способам продуцирования таких ферментов, кодирующим молекулам нуклеиновых кислот, рекомбинантным клеткам и способам трансформации биомассы за счет таких материалов. Изобретение, в частности, подходит для расщепления биомассы и/или увеличения расщепления биомассы и продуцирования биоэнергетических продуктов или рекомбинантных белков. Изобретение также относится к различным применениям ферментов в области бумажной промышленности, текстильной промышленности, а также в химической области и области медицины.
EA201390667A 2010-11-08 2011-11-08 Ферменты и их применения EA201390667A1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41116710P 2010-11-08 2010-11-08
EP10306221 2010-11-08
PCT/EP2011/069669 WO2012062767A2 (en) 2010-11-08 2011-11-08 Enzymes and uses thereof

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Publication Number Publication Date
EA201390667A1 true EA201390667A1 (ru) 2013-11-29

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EA201390667A EA201390667A1 (ru) 2010-11-08 2011-11-08 Ферменты и их применения

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US (2) US9279113B2 (ru)
EP (1) EP2638170B1 (ru)
JP (1) JP2013544086A (ru)
CN (1) CN103403172A (ru)
AU (1) AU2011328211A1 (ru)
CA (1) CA2816286A1 (ru)
EA (1) EA201390667A1 (ru)
SG (1) SG190161A1 (ru)
WO (1) WO2012062767A2 (ru)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140356923A1 (en) * 2011-12-23 2014-12-04 Deinove Bacteria with reconstructed transcriptional units and the uses thereof
DK3122876T3 (da) 2014-03-28 2021-02-08 Danisco Us Inc Ændret værtscellebane for forbedret ethanolproduktion
WO2016097324A1 (en) * 2014-12-19 2016-06-23 Deinove Alpha-galactosidases and uses thereof
CN114540318B (zh) * 2021-11-01 2024-02-02 北京化工大学 具有催化乙醇醛合成乙醇酸功能的酶及其应用
CN117925577A (zh) * 2024-03-19 2024-04-26 金堂佰信维科技有限公司 提高木聚糖酶活性的方法和木聚糖酶及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034644A1 (en) * 1994-06-15 1995-12-21 Novo Nordisk A/S Pyrodictium xylanase, amylase and pullulanase
JP3845697B2 (ja) 2002-02-22 2006-11-15 独立行政法人 日本原子力研究開発機構 放射線抵抗性細菌／大腸菌シャトルベクター
FR2923491B1 (fr) * 2007-11-14 2012-12-14 Deinove Sa Utilisation de bacteries pour la production de sources de bioenergie
EP2218773A1 (en) * 2009-02-17 2010-08-18 Deinove Compositions and methods for degrading lignocellulosic biomass

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US20130280786A1 (en) 2013-10-24
EP2638170B1 (en) 2017-10-18
SG190161A1 (en) 2013-06-28
AU2011328211A1 (en) 2013-05-30
JP2013544086A (ja) 2013-12-12
US9279113B2 (en) 2016-03-08
US20160168581A1 (en) 2016-06-16
WO2012062767A3 (en) 2012-07-05
CN103403172A (zh) 2013-11-20
WO2012062767A2 (en) 2012-05-18
CA2816286A1 (en) 2012-05-18
EP2638170A2 (en) 2013-09-18

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