MX2016012822A - Metodo para reducir el contenido de acido desoxirribonucleico (adn) de un caldo de fermentacion. - Google Patents
Metodo para reducir el contenido de acido desoxirribonucleico (adn) de un caldo de fermentacion.Info
- Publication number
- MX2016012822A MX2016012822A MX2016012822A MX2016012822A MX2016012822A MX 2016012822 A MX2016012822 A MX 2016012822A MX 2016012822 A MX2016012822 A MX 2016012822A MX 2016012822 A MX2016012822 A MX 2016012822A MX 2016012822 A MX2016012822 A MX 2016012822A
- Authority
- MX
- Mexico
- Prior art keywords
- reducing
- fermentation broth
- dna content
- broth
- dna
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/82—Asparaginase (3.5.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/01001—Asparaginase (3.5.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se describe un método para reducir el nivel de ADN en un caldo de fermentación donde el método incluye una etapa de calentamiento donde el caldo se calienta a una temperatura de al menos 70°C.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14166721 | 2014-04-30 | ||
PCT/EP2015/059497 WO2015166037A1 (en) | 2014-04-30 | 2015-04-30 | Method for reducing the dna content of a fermentation broth |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2016012822A true MX2016012822A (es) | 2016-12-09 |
Family
ID=50679872
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016012822A MX2016012822A (es) | 2014-04-30 | 2015-04-30 | Metodo para reducir el contenido de acido desoxirribonucleico (adn) de un caldo de fermentacion. |
Country Status (8)
Country | Link |
---|---|
US (1) | US10259841B2 (es) |
EP (1) | EP3137483B1 (es) |
CN (1) | CN106255698B (es) |
CA (1) | CA2941536C (es) |
DK (1) | DK3137483T3 (es) |
MX (1) | MX2016012822A (es) |
RU (1) | RU2687153C2 (es) |
WO (1) | WO2015166037A1 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109251872A (zh) * | 2018-07-31 | 2019-01-22 | 北京林业大学 | 废弃物堆肥用复合菌剂、其制备方法及废弃物堆肥方法 |
CN111040966B (zh) * | 2019-12-23 | 2022-06-24 | 河北科技大学 | 一种地衣芽孢杆菌KD-1、其生产的β-甘露聚糖酶及其应用 |
EP3926039A1 (en) | 2020-06-17 | 2021-12-22 | AB Enzymes GmbH | Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1149160B1 (en) * | 1999-01-25 | 2006-04-19 | Novozymes A/S | Recovery of a protein at high ph |
AU2003281982A1 (en) * | 2002-11-26 | 2004-06-18 | Novo Nordisk A/S | Process for purifying a fermentation-derived product |
RU2337968C2 (ru) | 2003-01-09 | 2008-11-10 | Дженентек, Инк. | Способ очистки гетерологичного полипептида |
US20050176090A1 (en) * | 2003-10-31 | 2005-08-11 | Novozymes A/S | Method for flocculation of a fermentation broth comprising a fungus |
DE602005020258D1 (de) * | 2004-02-27 | 2010-05-12 | Dow Global Technologies Inc | Verfahren zur extraktion eines intrazellulären proteins aus einer fermentationsbrühe |
EP2089422A1 (en) * | 2006-11-27 | 2009-08-19 | Natimmune A/S | Methods of separating nucleic acids and protein |
US20140212885A1 (en) * | 2011-09-22 | 2014-07-31 | Danisco Us Inc. | Endogenous dnase activity to reduce dna content |
US9249432B2 (en) * | 2012-07-13 | 2016-02-02 | Alliance For Sustainable Energy, Llc | Enzymes for improved biomass conversion |
-
2015
- 2015-04-30 DK DK15722474.2T patent/DK3137483T3/en active
- 2015-04-30 RU RU2016138692A patent/RU2687153C2/ru active
- 2015-04-30 US US15/306,941 patent/US10259841B2/en active Active
- 2015-04-30 MX MX2016012822A patent/MX2016012822A/es active IP Right Grant
- 2015-04-30 EP EP15722474.2A patent/EP3137483B1/en active Active
- 2015-04-30 WO PCT/EP2015/059497 patent/WO2015166037A1/en active Application Filing
- 2015-04-30 CA CA2941536A patent/CA2941536C/en active Active
- 2015-04-30 CN CN201580022280.9A patent/CN106255698B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
EP3137483B1 (en) | 2019-01-16 |
CA2941536C (en) | 2023-01-17 |
WO2015166037A1 (en) | 2015-11-05 |
US20170044209A1 (en) | 2017-02-16 |
CN106255698A (zh) | 2016-12-21 |
RU2687153C2 (ru) | 2019-05-07 |
RU2016138692A3 (es) | 2018-10-11 |
RU2016138692A (ru) | 2018-05-30 |
CA2941536A1 (en) | 2015-11-05 |
EP3137483A1 (en) | 2017-03-08 |
US10259841B2 (en) | 2019-04-16 |
DK3137483T3 (en) | 2019-04-23 |
CN106255698B (zh) | 2021-08-03 |
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