EP2928496A4 - Modification et régulation du génome basées sur crispr - Google Patents

Modification et régulation du génome basées sur crispr Download PDF

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Publication number
EP2928496A4
EP2928496A4 EP13859964.2A EP13859964A EP2928496A4 EP 2928496 A4 EP2928496 A4 EP 2928496A4 EP 13859964 A EP13859964 A EP 13859964A EP 2928496 A4 EP2928496 A4 EP 2928496A4
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EP
European Patent Office
Prior art keywords
crispr
regulation
genome modification
based genome
modification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP13859964.2A
Other languages
German (de)
English (en)
Other versions
EP2928496B1 (fr
EP2928496A1 (fr
Inventor
Fuqiang Chen
Gregory D. Davis
Qiaohua KANG
Scott W. KNIGHT
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Aldrich Co LLC
Original Assignee
Sigma Aldrich Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50883989&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2928496(A4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP18160519.7A priority Critical patent/EP3363902B1/fr
Priority to EP16183717.4A priority patent/EP3138909A1/fr
Priority to EP16183724.0A priority patent/EP3138911B1/fr
Priority to DK16183723.2T priority patent/DK3138910T3/en
Priority to EP16183725.7A priority patent/EP3138912B1/fr
Priority to DK18160519.7T priority patent/DK3363902T3/da
Priority to EP16183719.0A priority patent/EP3135765A1/fr
Priority to PL16183723T priority patent/PL3138910T3/pl
Priority to PL16183725T priority patent/PL3138912T3/pl
Priority to DK18156734T priority patent/DK3360964T3/da
Priority to PL18156734T priority patent/PL3360964T3/pl
Priority to EP16183720.8A priority patent/EP3141604A1/fr
Priority to EP16183723.2A priority patent/EP3138910B1/fr
Application filed by Sigma Aldrich Co LLC filed Critical Sigma Aldrich Co LLC
Priority to PL16183724T priority patent/PL3138911T3/pl
Priority to PL18160519T priority patent/PL3363902T3/pl
Priority to EP18156734.8A priority patent/EP3360964B1/fr
Priority to DK16183725.7T priority patent/DK3138912T3/en
Priority to PL13859964T priority patent/PL2928496T3/pl
Priority to EP19189913.7A priority patent/EP3617309A3/fr
Priority to DK16183724.0T priority patent/DK3138911T3/en
Priority to EP19201769.7A priority patent/EP3611263A1/fr
Publication of EP2928496A1 publication Critical patent/EP2928496A1/fr
Publication of EP2928496A4 publication Critical patent/EP2928496A4/fr
Publication of EP2928496B1 publication Critical patent/EP2928496B1/fr
Application granted granted Critical
Revoked legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0048—Eye, e.g. artificial tears
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/10—Ophthalmic agents for accommodation disorders, e.g. myopia
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/463—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from amphibians
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Enzymes And Modification Thereof (AREA)
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EP13859964.2A 2012-12-06 2013-12-05 Modification et régulation du génome basées sur crispr Revoked EP2928496B1 (fr)

Priority Applications (21)

Application Number Priority Date Filing Date Title
EP19189913.7A EP3617309A3 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183724.0A EP3138911B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
DK16183723.2T DK3138910T3 (en) 2012-12-06 2013-12-05 CRISPR-BASED RE-MODIFICATION AND REGULATION
EP16183725.7A EP3138912B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
DK18160519.7T DK3363902T3 (da) 2012-12-06 2013-12-05 Crispr-baseret genommodifikation og -regulering
EP16183719.0A EP3135765A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
PL16183723T PL3138910T3 (pl) 2012-12-06 2013-12-05 Oparta na CRISPR modyfikacja i regulacja genomu
PL16183725T PL3138912T3 (pl) 2012-12-06 2013-12-05 Modyfikacja i regulacja genomu oparta na CRISPR
DK18156734T DK3360964T3 (da) 2012-12-06 2013-12-05 Crispr-baseret genommodifikation og -regulering
PL18156734T PL3360964T3 (pl) 2012-12-06 2013-12-05 Modyfikacja i regulacja genomu oparta na crispr
EP16183720.8A EP3141604A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183723.2A EP3138910B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP18160519.7A EP3363902B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
PL13859964T PL2928496T3 (pl) 2012-12-06 2013-12-05 Modyfikacja i regulacja genomu w oparciu o CRISPR
PL18160519T PL3363902T3 (pl) 2012-12-06 2013-12-05 Modyfikacja i regulacja genomu w oparciu o CRISPR
EP18156734.8A EP3360964B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
DK16183725.7T DK3138912T3 (en) 2012-12-06 2013-12-05 CRISPR-BASED RE-MODIFICATION AND REGULATION
PL16183724T PL3138911T3 (pl) 2012-12-06 2013-12-05 Modyfikacja i regulacja genomu oparta na CRISPR
EP16183717.4A EP3138909A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
DK16183724.0T DK3138911T3 (en) 2012-12-06 2013-12-05 CRISPR-BASED RE-MODIFICATION AND REGULATION
EP19201769.7A EP3611263A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261734256P 2012-12-06 2012-12-06
US201361758624P 2013-01-30 2013-01-30
US201361761046P 2013-02-05 2013-02-05
US201361794422P 2013-03-15 2013-03-15
PCT/US2013/073307 WO2014089290A1 (fr) 2012-12-06 2013-12-05 Modification et régulation du génome basées sur crispr

Related Child Applications (19)

Application Number Title Priority Date Filing Date
EP16183723.2A Division-Into EP3138910B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183723.2A Division EP3138910B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183720.8A Division-Into EP3141604A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183720.8A Division EP3141604A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP19201769.7A Division EP3611263A1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP18156734.8A Division-Into EP3360964B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP18156734.8A Division EP3360964B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183725.7A Division EP3138912B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP16183725.7A Division-Into EP3138912B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP18160519.7A Division-Into EP3363902B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
EP18160519.7A Division EP3363902B1 (fr) 2012-12-06 2013-12-05 Modification et régulation de génome à base de crispr
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EP3138912A1 (fr) 2017-03-08
DK2928496T3 (da) 2019-11-11
PT2928496T (pt) 2019-11-11
EP3135765A1 (fr) 2017-03-01
KR20200098727A (ko) 2020-08-20
JP2021101706A (ja) 2021-07-15
US20160298133A1 (en) 2016-10-13
KR102479178B1 (ko) 2022-12-19
ES2713243T3 (es) 2019-05-20
CA2977152A1 (fr) 2014-06-12
CN108913676B (zh) 2023-11-03
AU2019201344B2 (en) 2020-09-03
US20210079427A1 (en) 2021-03-18
LT3138910T (lt) 2017-11-10
KR102145760B1 (ko) 2020-08-19
EP2928496B1 (fr) 2019-10-09
US20210388396A1 (en) 2021-12-16
US20210207173A1 (en) 2021-07-08
JP7478772B2 (ja) 2024-05-07
IL267598A (en) 2019-08-29
IL267598B (en) 2022-05-01
PT3138912T (pt) 2018-12-28
ES2757325T3 (es) 2020-04-28
EP3617309A2 (fr) 2020-03-04
PL3360964T3 (pl) 2020-03-31
US20160298138A1 (en) 2016-10-13
SG10202107423UA (en) 2021-08-30
US20160298132A1 (en) 2016-10-13
AU2013355214B2 (en) 2017-06-15
AU2020273316B2 (en) 2023-05-18
KR102006880B1 (ko) 2019-08-02
EP3611263A1 (fr) 2020-02-19
AU2022200330A1 (en) 2022-02-17
AU2018229489B2 (en) 2018-12-06
AU2019201344C1 (en) 2020-12-24
PL3363902T3 (pl) 2020-05-18
AU2013355214A1 (en) 2015-06-04
EP3138911A1 (fr) 2017-03-08
IL238856A0 (en) 2015-06-30
LT3138911T (lt) 2019-02-25
CN108913676A (zh) 2018-11-30
AU2019201344A1 (en) 2019-03-21
AU2018229489A1 (en) 2018-10-04
ES2769310T3 (es) 2020-06-25
KR20230070065A (ko) 2023-05-19
US20160017366A1 (en) 2016-01-21
EP3363902B1 (fr) 2019-11-27
CN105142669A (zh) 2015-12-09
PT3363902T (pt) 2019-12-19
AU2022200330B2 (en) 2023-11-09
EP3138911B1 (fr) 2018-12-05
IL300199A (en) 2023-03-01
DK3138910T3 (en) 2017-10-16
PL3138910T3 (pl) 2018-01-31
CA2891347A1 (fr) 2014-06-12
AU2020230246A1 (en) 2020-10-01
EP2928496A1 (fr) 2015-10-14
US10745716B2 (en) 2020-08-18
KR20150091052A (ko) 2015-08-07
DK3360964T3 (da) 2019-10-28
EP3363902A1 (fr) 2018-08-22
KR102531576B1 (ko) 2023-05-11
US20160298134A1 (en) 2016-10-13
PL2928496T3 (pl) 2020-04-30
HK1218389A1 (zh) 2017-02-17
LT3138912T (lt) 2019-02-25
JP6620018B2 (ja) 2019-12-11
KR102243092B1 (ko) 2021-04-22
EP3138912B1 (fr) 2018-12-05
JP2017192392A (ja) 2017-10-26
IL291129B1 (en) 2023-03-01
BR112015012375A2 (pt) 2017-09-26
KR20190093680A (ko) 2019-08-09
US20160298137A1 (en) 2016-10-13
ES2757808T3 (es) 2020-04-30
SG10201910987SA (en) 2020-01-30
IL291129B2 (en) 2023-07-01
AU2017204031A1 (en) 2017-07-06
CN108715602A (zh) 2018-10-30
PT3138911T (pt) 2018-12-28
WO2014089290A1 (fr) 2014-06-12
IL257178A (en) 2018-03-29
EP3138910A1 (fr) 2017-03-08
DK3138912T3 (en) 2019-01-21
EP3138909A1 (fr) 2017-03-08
PL3138911T3 (pl) 2019-04-30
LT3363902T (lt) 2020-02-10
KR20230003624A (ko) 2023-01-06
EP3138910B1 (fr) 2017-09-20
PT3360964T (pt) 2019-10-29
KR20180011351A (ko) 2018-01-31
ES2653212T3 (es) 2018-02-06
KR101844123B1 (ko) 2018-04-02
CA2891347C (fr) 2018-02-27
SG11201503824SA (en) 2015-06-29
KR20210045515A (ko) 2021-04-26
US20160298135A1 (en) 2016-10-13
ES2714154T3 (es) 2019-05-27
PL3138912T3 (pl) 2019-04-30
AU2020273316A1 (en) 2020-12-17
US20160298125A1 (en) 2016-10-13
CN105142669B (zh) 2018-07-03
AU2020230243B2 (en) 2021-10-21
CA2977152C (fr) 2019-04-09
CA3034794A1 (fr) 2014-06-12
IL291129A (en) 2022-05-01
US20170073705A1 (en) 2017-03-16
SG10201800585VA (en) 2018-02-27
IL238856B (en) 2018-03-29
JP2019037231A (ja) 2019-03-14
EP3617309A3 (fr) 2020-05-06
JP2020120674A (ja) 2020-08-13
EP3141604A1 (fr) 2017-03-15
EP3360964A1 (fr) 2018-08-15
DK3363902T3 (da) 2020-01-02
US20200140897A1 (en) 2020-05-07
US20160298136A1 (en) 2016-10-13
AU2017204031B2 (en) 2018-06-14
AU2023216829A1 (en) 2023-09-14
AU2020230246B2 (en) 2020-11-05
JP2016502840A (ja) 2016-02-01
DK3138911T3 (en) 2019-01-21
PT3138910T (pt) 2017-10-18
US10731181B2 (en) 2020-08-04
EP3360964B1 (fr) 2019-10-02
IL257178B (en) 2019-07-31
JP2022115994A (ja) 2022-08-09

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