EP2626436A3 - Aptamères chimiquement modifiés avec des vitesses de dissocation améliorées - Google Patents

Aptamères chimiquement modifiés avec des vitesses de dissocation améliorées Download PDF

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Publication number
EP2626436A3
EP2626436A3 EP13164719.0A EP13164719A EP2626436A3 EP 2626436 A3 EP2626436 A3 EP 2626436A3 EP 13164719 A EP13164719 A EP 13164719A EP 2626436 A3 EP2626436 A3 EP 2626436A3
Authority
EP
European Patent Office
Prior art keywords
target
nucleic acid
detecting
rates
slow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13164719.0A
Other languages
German (de)
English (en)
Other versions
EP2626436A2 (fr
Inventor
Daniel J. Schneider
Dan Nieuwlandt
Bruce Eaton
Marty Stanton
Shashi Gupta
Stephan Kraemer
Dominic Zichi
Larry Gold
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Somalogic Inc
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40260081&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2626436(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of EP2626436A2 publication Critical patent/EP2626436A2/fr
Publication of EP2626436A3 publication Critical patent/EP2626436A3/fr
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
EP13164719.0A 2007-07-17 2008-07-17 Aptamères chimiquement modifiés avec des vitesses de dissocation améliorées Withdrawn EP2626436A3 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US95028307P 2007-07-17 2007-07-17
US95029307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US3142008P 2008-02-26 2008-02-26
US5159408P 2008-05-08 2008-05-08
EP08782013.0A EP2076613B1 (fr) 2007-07-17 2008-07-17 Analyses d'échantillons d'essai

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP08782013.0 Division 2008-07-17
EP08782013.0A Division-Into EP2076613B1 (fr) 2007-07-17 2008-07-17 Analyses d'échantillons d'essai

Publications (2)

Publication Number Publication Date
EP2626436A2 EP2626436A2 (fr) 2013-08-14
EP2626436A3 true EP2626436A3 (fr) 2013-10-16

Family

ID=40260081

Family Applications (10)

Application Number Title Priority Date Filing Date
EP13164719.0A Withdrawn EP2626436A3 (fr) 2007-07-17 2008-07-17 Aptamères chimiquement modifiés avec des vitesses de dissocation améliorées
EP08781998A Withdrawn EP2069496A4 (fr) 2007-07-17 2008-07-17 Selex et photoselex améliorés
EP08782010A Active EP2069529B1 (fr) 2007-07-17 2008-07-17 Procede pour generer des aptameres avec des vitesses de dissociation ameliorees
EP17181688.7A Active EP3284832B1 (fr) 2007-07-17 2008-07-17 Aptamère ayant une vitesse de dissociation améliorée, et son complexe aptamère-cible non-covalent
EP08782013.0A Active EP2076613B1 (fr) 2007-07-17 2008-07-17 Analyses d'échantillons d'essai
EP15152547.4A Active EP2933340B1 (fr) 2007-07-17 2008-07-17 Aptamères avec uridines et/ou thymidines substituées à la position 5 par un groupement benzyl
EP22162947.0A Pending EP4056711A1 (fr) 2007-07-17 2008-07-17 Procédé de génération d'aptamères ayant des vitesses d'arrêt améliorées
EP10176864A Withdrawn EP2336314A1 (fr) 2007-07-17 2008-07-17 Selex et photoselex améliorés
EP09012809.1A Active EP2172566B2 (fr) 2007-07-17 2008-07-17 Procédé de génération d'aptamères avec des vitesses d'arrêt améliorées
EP12160299.9A Active EP2489743B1 (fr) 2007-07-17 2008-07-17 Aptamères avec uridines substituées par 5-(N-naphthyl)

Family Applications After (9)

Application Number Title Priority Date Filing Date
EP08781998A Withdrawn EP2069496A4 (fr) 2007-07-17 2008-07-17 Selex et photoselex améliorés
EP08782010A Active EP2069529B1 (fr) 2007-07-17 2008-07-17 Procede pour generer des aptameres avec des vitesses de dissociation ameliorees
EP17181688.7A Active EP3284832B1 (fr) 2007-07-17 2008-07-17 Aptamère ayant une vitesse de dissociation améliorée, et son complexe aptamère-cible non-covalent
EP08782013.0A Active EP2076613B1 (fr) 2007-07-17 2008-07-17 Analyses d'échantillons d'essai
EP15152547.4A Active EP2933340B1 (fr) 2007-07-17 2008-07-17 Aptamères avec uridines et/ou thymidines substituées à la position 5 par un groupement benzyl
EP22162947.0A Pending EP4056711A1 (fr) 2007-07-17 2008-07-17 Procédé de génération d'aptamères ayant des vitesses d'arrêt améliorées
EP10176864A Withdrawn EP2336314A1 (fr) 2007-07-17 2008-07-17 Selex et photoselex améliorés
EP09012809.1A Active EP2172566B2 (fr) 2007-07-17 2008-07-17 Procédé de génération d'aptamères avec des vitesses d'arrêt améliorées
EP12160299.9A Active EP2489743B1 (fr) 2007-07-17 2008-07-17 Aptamères avec uridines substituées par 5-(N-naphthyl)

Country Status (18)

Country Link
US (1) US8409795B2 (fr)
EP (10) EP2626436A3 (fr)
JP (11) JP5684568B2 (fr)
KR (3) KR101656240B1 (fr)
CN (6) CN101809167B (fr)
AU (3) AU2008275917B2 (fr)
CA (4) CA2696431C (fr)
DK (4) DK2489743T3 (fr)
ES (5) ES2604764T3 (fr)
FI (1) FI3284832T3 (fr)
HK (6) HK1133046A1 (fr)
HR (1) HRP20150382T1 (fr)
MX (6) MX2010000533A (fr)
NO (1) NO2933340T3 (fr)
PL (1) PL2489743T3 (fr)
PT (2) PT2489743E (fr)
SI (1) SI2489743T1 (fr)
WO (3) WO2009012410A1 (fr)

Families Citing this family (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5993279A (ja) * 1982-11-17 1984-05-29 富士通株式会社 ロボツトによるカ−ド，レシ−ト分離取出し方法
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8404830B2 (en) * 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
CN101809167B (zh) * 2007-07-17 2015-04-01 私募蛋白质体公司 产生具有改良的解离速率的适配体的方法
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
JP5766178B2 (ja) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ ＳｌｉｐＣｈｉｐ装置および方法
US8569252B2 (en) * 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
AU2013203588C1 (en) * 2009-07-09 2016-06-30 Somalogic Operating Co., Inc. Method for generating aptamers with improved off-rates
JP5967687B2 (ja) * 2009-07-17 2016-08-10 Ｎｅｃソリューションイノベータ株式会社 スフィンゴシルホスホリルコリンに結合するアプタマー分子
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US9453845B2 (en) 2010-02-01 2016-09-27 Cell Signaling Technology, Inc. Mass spectroscopy analysis of mutant polypeptides in biological samples
RU2615143C2 (ru) * 2010-03-24 2017-04-04 Адвирна Самодоставляющие PHKi соединения уменьшенного размера
EP2558478B1 (fr) * 2010-04-12 2018-03-07 Somalogic, Inc. Pyrimidines modifiées en position 5 et leur utilisation
AU2013202528B2 (en) * 2010-04-12 2015-07-30 Somalogic Operating Co., Inc. 5-position modified pyrimidines and their use
EP2591357A4 (fr) * 2010-07-09 2014-01-01 Somalogic Inc Biomarqueurs du cancer du poumon et leurs utilisations
CA2809282C (fr) 2010-09-27 2017-09-12 Somalogic, Inc. Biomarqueurs de mesotheliome et utilisations de ceux-ci
EP2635681B8 (fr) * 2010-11-05 2017-10-04 Miragen Therapeutics, Inc. Oligonucléotides à bases modifiées
KR101307616B1 (ko) * 2011-02-08 2013-09-12 경희대학교 산학협력단 Ｐｎａ 앱타머를 이용하여 금속을 분리하는 방법
JP5805788B2 (ja) * 2011-12-28 2015-11-10 シスメックス株式会社 副腎皮質刺激ホルモンと結合する分子およびその利用
MX355567B (es) 2011-12-30 2018-04-23 Quest Diagnostics Invest Inc Aptameros y metodos de diagnostico para detectar el receptor de egf.
CN104379764B (zh) 2012-03-28 2018-04-06 私募蛋白质体公司 Pdgf和vegf的适体及它们在治疗pdgf和vegf介导的病状中的用途
US9617589B2 (en) * 2012-05-25 2017-04-11 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
MY170164A (en) * 2012-06-07 2019-07-09 Somalogic Inc Aptamer-based multiplexed assays
CN102719430B (zh) * 2012-06-13 2013-06-12 湖南大学 一种检测组氨酸标签重组蛋白的核酸适配体分子信标探针及其检测方法
KR101556339B1 (ko) * 2012-07-02 2015-10-13 국립대학법인 울산과학기술대학교 산학협력단 페리오스틴에 대한 압타머 및 이를 포함하는 항암제 조성물
EP2690441A1 (fr) * 2012-07-26 2014-01-29 Rheinische Friedrich-Wilhelms-Universität Bonn Procédé de détermination d'une protéine dans un échantillon
CN102912020B (zh) * 2012-10-20 2014-02-19 江南大学 一种测定赭曲霉毒素a的适配体传感器的构建方法
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
EP4170031A1 (fr) 2012-10-23 2023-04-26 Caris Science, Inc. Aptamères et leurs utilisations
WO2014074682A1 (fr) 2012-11-07 2014-05-15 Somalogic, Inc. Biomarqueurs de la bronchopneumopathie chronique obstructive (bpco) et leurs utilisations
AU2013361323B2 (en) 2012-12-19 2018-09-06 Caris Science, Inc. Compositions and methods for aptamer screening
SG11201507335QA (en) 2013-03-14 2015-10-29 Somalogic Inc Aptamers that bind to il-6 and their use in treating or diagnosing il-6 mediated conditions
WO2014144744A1 (fr) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Procédés et compositions se rapportant à des aptamères
MX360140B (es) 2013-03-15 2018-10-24 Somalogic Inc Biomarcadores de la enfermedad del higado graso no alcoholico (nafld) y la esteatohepatitis no alcoholica (nash) y usos de estos.
JP6034261B2 (ja) * 2013-08-02 2016-11-30 日本電信電話株式会社 生体分子検出用チップの製造方法
EP3044334B1 (fr) 2013-09-09 2020-08-12 Somalogic, Inc. Aptamères de pdgf et vegf présentant une stabilité améliorée et leur utilisation dans le traitement de maladies et de troubles médiés par pdgf et vegf
JP6506768B2 (ja) 2013-09-24 2019-04-24 ソマロジック・インコーポレーテッド 多重アプタマー標的の検出
ES2714324T3 (es) * 2013-10-02 2019-05-28 Fabian Tolle Un método para identificar o producir un aptámero
US9938314B2 (en) 2013-11-21 2018-04-10 Somalogic, Inc. Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
ES2764669T3 (es) 2014-02-18 2020-06-04 Somalogic Inc Composiciones y métodos para la detección de microorganismos
WO2015164616A1 (fr) 2014-04-24 2015-10-29 Somalogic, Inc. Biomarqueurs de détection de la tuberculose
WO2015164617A1 (fr) 2014-04-24 2015-10-29 Somalogic, Inc. Biomarqueurs de la tuberculose dans l'urine et leurs utilisations
WO2016000216A1 (fr) * 2014-07-01 2016-01-07 深圳迈瑞生物医疗电子股份有限公司 Méthode d'analyse d'échantillon de sang total et dispositif d'analyse de sang
US10670611B2 (en) 2014-09-26 2020-06-02 Somalogic, Inc. Cardiovascular risk event prediction and uses thereof
KR101849557B1 (ko) * 2014-09-29 2018-04-17 동국대학교 산학협력단 신규 세포 타겟 압타머 제조방법
EP3201353B1 (fr) * 2014-10-02 2019-12-25 Rheinische Friedrich-Wilhelms-Universität Bonn Procédé d'identification ou de production d'un aptamère
KR101719285B1 (ko) 2014-11-04 2017-03-23 한국과학기술원 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법
TWI695068B (zh) 2014-11-24 2020-06-01 美商身體邏輯股份有限公司 用於結合生長分化因子１１的核酸化合物
CN104360077A (zh) * 2014-11-25 2015-02-18 重庆市科学技术研究院 一种用于检测多西环素残留的适配体核酸探针试剂盒、制备方法及其应用
WO2016123058A1 (fr) 2015-01-27 2016-08-04 Somalogic, Inc. Biomarqueurs pour la détection du risque de tuberculose
US10370669B2 (en) 2015-02-09 2019-08-06 Somalogic, Inc. Nucleic acid compounds for binding growth differentiation factor 8
US10138486B2 (en) 2015-03-12 2018-11-27 The Regents Of The University Of Michigan Inhibitors of DEK protein and related methods
EP3295176A1 (fr) 2015-05-08 2018-03-21 Somalogic, Inc. Biomarqueurs pour la détection du risque de tuberculose
US20180259533A1 (en) 2015-09-09 2018-09-13 Somalogic, Inc. Methods for developing personalized drug treatment plans and targeted drug development based on proteomic profiles
CA3002073A1 (fr) * 2015-10-20 2017-04-27 Sorrento Therapeutics, Inc. Composes d'administration intracellulaire
US20210215711A1 (en) 2016-02-08 2021-07-15 Somalogic, Inc. Nonalcoholic Fatty Liver Disease (NAFLD) and Nonalcoholic Steatohepatitis (NASH) Biomarkers and Uses Thereof
GB201603789D0 (en) * 2016-03-04 2016-04-20 Apta Biosciences Ltd Oligonucleotides and methods for preparing
EP3913058A1 (fr) 2016-07-01 2021-11-24 Somalogic, Inc. Oligonucléotides comportant des nucléosides modifiés
CN106248767B (zh) * 2016-07-15 2018-12-07 济南大学 一种用于检测癌细胞中h2s的三维纸分析器件的制备方法
CN107663220B (zh) * 2016-07-27 2020-10-02 上海伯豪医学检验所有限公司 修饰碱基、包含修饰碱基的核酸、适配体及其应用
TW201821609A (zh) * 2016-08-22 2018-06-16 Nissan Chemical Ind Ltd 使用ｅ－鈣黏蛋白結合型核酸適體之細胞貼附材料
US11041849B2 (en) * 2016-12-01 2021-06-22 Aptitude Medical Systems, Inc. Methods and systems for identifying candidate nucleic acid agent
KR101993427B1 (ko) * 2017-04-26 2019-10-01 주식회사 압타머사이언스 백혈구에 선택적으로 결합하는 압타머 및 이의 용도
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
WO2019113506A1 (fr) 2017-12-07 2019-06-13 The Broad Institute, Inc. Procédés et compositions pour multiplexer un séquençage de noyaux isolés et de cellules isolées
US10481155B2 (en) * 2018-03-09 2019-11-19 Somalogic, Inc. Proteomic assay using quantum sensors
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
US11231420B2 (en) 2018-04-09 2022-01-25 Aptalogic, Inc. Selection and optimization of aptamers to recognize ebola markers
US20210140977A1 (en) 2018-04-16 2021-05-13 University Of Cape Town A three-protein proteomic biomarker for prospective determination of risk for development of active tuberculosis
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
BR112020026129B1 (pt) * 2018-06-22 2023-10-10 Somalogic Operating Co., Inc Métodos para melhorar o desempenho de ensaios proteômicos multiplex
WO2020037250A2 (fr) * 2018-08-17 2020-02-20 The Trustees Of Columbia University In The City Of New York Détection cible de dispositifs capteurs à transistors à effet de champ à base de récepteurs en épingle à cheveux pour petites molécules dans des concentrations physiologiques en sels
US11313855B2 (en) 2018-09-25 2022-04-26 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using conjugated molecular traps
AU2019352901A1 (en) 2018-10-02 2021-05-27 WearOptimo Pty Ltd Measurement system
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
US20210317456A1 (en) 2018-10-15 2021-10-14 Somalogic, Inc. Nucleic Acid Compounds for Binding Immunoglobulin G
CN113227395A (zh) 2018-12-04 2021-08-06 豪夫迈·罗氏有限公司 细胞靶标的空间上定向的量子条形编码
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
US20220142948A1 (en) 2019-03-18 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
EP3969900A1 (fr) * 2019-05-17 2022-03-23 Somalogic, Inc. Contrôle de la variabilité d'analytes inter-échantillons dans des matrices biologiques complexes
US20220243178A1 (en) 2019-05-31 2022-08-04 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
US11198908B2 (en) 2019-06-17 2021-12-14 Biorchestra Co., Ltd. Method for diagnosis of Alzheimer's disease using microRNA
KR102113078B1 (ko) * 2019-07-05 2020-05-20 광주과학기술원 압타머의 선별 방법
US20220282333A1 (en) 2019-08-13 2022-09-08 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
CN110396536A (zh) * 2019-08-14 2019-11-01 东南大学 一种基于分支滚环扩增的外泌体荧光检测传感器
US20220349904A1 (en) 2019-09-03 2022-11-03 Somalogic Operating Co., Inc. Cardiovascular Risk Event Prediction and Uses Thereof
CN110618265B (zh) * 2019-09-12 2023-04-18 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） 一种沙丁胺醇的可视化检测方法
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
WO2021076713A1 (fr) 2019-10-16 2021-04-22 Somalogic, Inc. Composés d'acide nucléique qui se lient à la protéine i du gène inductible par l'acide rétinoïque
US20230093390A1 (en) * 2019-12-03 2023-03-23 Sung Chun Kim Method for obtaining profile of target molecule population of sample
JP2023509677A (ja) 2020-01-10 2023-03-09 ソマロジック オペレーティング カンパニー インコーポレイテッド 耐糖能異常障害を決定する方法
AU2021220787A1 (en) 2020-02-10 2022-09-01 Somalogic Operating Co., Inc. Nonalcoholic steatohepatitis (NASH) biomarkers and uses thereof
CN111304202B (zh) * 2020-02-26 2023-03-28 西安交通大学 一种用于识别且增强hmgb1生物学活性的核酸适配子及其应用
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
CA3177923A1 (fr) * 2020-05-18 2021-11-25 Shanghai Polaris Biology Co., Ltd. Microbilles et leurs utilisations
CN111735869A (zh) * 2020-05-29 2020-10-02 中山大学 一种蛋白质的检测试剂及检测方法
EP4232825A2 (fr) 2020-10-20 2023-08-30 SomaLogic Operating Co., Inc. Prédiction de risque d'événement cardiovasculaire
MX2023012081A (es) 2021-04-13 2023-10-25 Somalogic Operating Co Inc Nucleosidos modificados.
US20240218374A1 (en) 2021-04-14 2024-07-04 Somalogic Operating Co., Inc. Nucleic Acid Compounds That Bind Coronavirus Proteins
WO2022235957A2 (fr) * 2021-05-06 2022-11-10 Systems Oncology, Llc Compositions d'immunothérapie d'arn à ciblage multiple
US20240255524A1 (en) 2021-06-15 2024-08-01 Somalogic Operating Co., Inc. Renal Insufficiency Prediction and Uses Thereof
WO2023059854A1 (fr) 2021-10-07 2023-04-13 Somalogic Operating Co., Inc. Prédiction du cancer du poumon et utilisations associées
CN114058613B (zh) * 2021-11-18 2023-10-27 广州血液中心(中国医学科学院输血研究所广州分所、广州器官移植配型中心) 一种大体积、高灵敏度核酸提取方法
CN114295594B (zh) * 2021-12-06 2023-09-19 贵州理工学院 一种基于分子信标筛选三螺旋DNA嵌入剂的“turn on”型荧光传感器
CA3242172A1 (fr) 2022-01-21 2023-07-27 David ASTLING Procedes d'evaluation de qualite d'echantillons
WO2023183873A1 (fr) 2022-03-24 2023-09-28 Mensura Health Inc. Aptamères et leurs utilisations
WO2023211773A1 (fr) 2022-04-24 2023-11-02 Somalogic Operating Co., Inc. Procédés d'évaluation de qualité d'échantillons
AU2023260452A1 (en) 2022-04-24 2024-09-12 Somalogic Operating Co., Inc. Methods for sample quality assessment
WO2023211769A1 (fr) 2022-04-24 2023-11-02 Somalogic Operating Co., Inc. Procédés pour évaluater la qualité d'un échantillon
WO2023211771A1 (fr) 2022-04-24 2023-11-02 Somalogic Operating Co., Inc. Procédés d'évaluation de la qualité d'un échantillon
WO2024015485A1 (fr) 2022-07-14 2024-01-18 Somalogic Operating Co., Inc. Procédés d'évaluation du risque de démence
WO2024015486A1 (fr) 2022-07-14 2024-01-18 Somalogic Operating Co., Inc. Procédés d'évaluation de la qualité d'un échantillon
WO2024064322A2 (fr) 2022-09-23 2024-03-28 Somalogic Operating Co., Inc. Procédés d'évaluation de l'état d'utilisation de tabac
WO2024192141A1 (fr) 2023-03-13 2024-09-19 Dana-Farber Cancer Institute, Inc. Traitement de cancers présentant un état de cellule mésenchymateuse résistant aux médicaments
EP4442282A1 (fr) 2023-04-07 2024-10-09 POSTECH Research and Business Development Foundation Préparation de conjugués polypeptidiques spécifiques d'un site

Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014843A1 (fr) * 1991-02-21 1992-09-03 Gilead Sciences, Inc. Aptamere specifique de biomolecules et procede de production
US5645985A (en) * 1991-11-26 1997-07-08 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US5945527A (en) * 1996-05-30 1999-08-31 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide
US6030776A (en) * 1990-06-11 2000-02-29 Nexstar Pharmaceuticals, Inc. Parallel SELEX
US6458543B1 (en) * 1997-12-15 2002-10-01 Somalogic, Incorporated Nucleic acid ligand diagnostic biochip
US6458539B1 (en) * 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
US20050130195A1 (en) * 2002-03-19 2005-06-16 Fujitsu Limited Functional molecule and process for producing the same
US20050158780A1 (en) * 2000-10-16 2005-07-21 Gilead Sciences, Inc. Nucleic acid ligands to the prostate specific membrane antigen
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US20060105378A1 (en) * 1999-07-29 2006-05-18 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
WO2007084886A2 (fr) * 2006-01-17 2007-07-26 Somalogic, Incorporated Analyses multiplexees d’echantillons test
WO2008078180A2 (fr) * 2006-12-22 2008-07-03 Archemix Corp. Matériaux et procédés pour la génération de transcrits comprenant des nucléotides modifiés

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CH0229046H1 (de) * 1985-03-30 1998-07-15 Stuart Alan Kauffman Method for obtaining dna, rna, peptides, polypeptinique. des or proteins by means of a dna recombinant tech
US4753983A (en) * 1986-05-07 1988-06-28 Bioprobe International, Inc. Polymeric matrix for affinity chromatography and immobilization of ligands
WO1989006694A1 (fr) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Procede de selection de substances proteiniques qui imitent des molecules d'induction de croissance
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5874557A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5962219A (en) 1990-06-11 1999-10-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-selex
US6083696A (en) * 1990-06-11 2000-07-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands exponential enrichment: blended selex
US5874218A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
US6168778B1 (en) 1990-06-11 2001-01-02 Nexstar Pharmaceuticals, Inc. Vascular endothelial growth factor (VEGF) Nucleic Acid Ligand Complexes
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US6001577A (en) * 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5763177A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
US6716580B2 (en) * 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US5472841A (en) * 1990-06-11 1995-12-05 Nexstar Pharmaceuticals, Inc. Methods for identifying nucleic acid ligands of human neutrophil elastase
US6346611B1 (en) * 1990-06-11 2002-02-12 Gilead Sciences, Inc. High affinity TGfβ nucleic acid ligands and inhibitors
US5763173A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US20040132067A1 (en) * 1990-06-11 2004-07-08 Somalogic, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5707796A (en) 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5459015A (en) * 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
ATE318832T1 (de) 1990-06-11 2006-03-15 Gilead Sciences Inc Verfahren zur vervendung von nukleinsäureliganden
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5789163A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US5853984A (en) 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
SE501013C2 (sv) 1990-10-01 1994-10-17 Pharmacia Biosensor Ab Förfarande för förbättring av analys med bindning till fast fas
AU2580892A (en) 1991-09-05 1993-04-05 Isis Pharmaceuticals, Inc. Determination of oligonucleotides for therapeutics, diagnostics and research reagents
US5312730A (en) * 1992-05-27 1994-05-17 Ciba Corning Diagnostics Corp. Immune complex transfer with lypophilic bridge
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5428149A (en) 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
US5580972A (en) 1993-06-14 1996-12-03 Nexstar Pharmaceuticals, Inc. Purine nucleoside modifications by palladium catalyzed methods
US5719273A (en) 1993-06-14 1998-02-17 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modifications methods using nucleophiles and carbon monoxide
US5998142A (en) * 1993-09-08 1999-12-07 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
EP1793006A3 (fr) * 1993-09-08 2007-08-22 Gilead Sciences, Inc. Ligands d'acide nucléique et méthode pour les produire
DE69435048T2 (de) 1993-09-17 2008-10-02 Somalogic, Inc., Boulder Systematische evolution von liganden durch exponentielle anreicherung: fotoselektion von nukleinsäureliganden
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
JP4899014B2 (ja) * 1995-06-02 2012-03-21 イーシー・テクノロジー・エルエルシー 求核試薬および一酸化炭素を用いるパラジウム触媒ヌクレオシド修飾方法
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
DE69637805D1 (de) 1995-06-07 2009-02-26 Gilead Sciences Inc Nukleinsäure liganden die dna-polymerase binden und inhibieren
US5737498A (en) 1995-07-11 1998-04-07 Beckman Instruments, Inc. Process automation method and apparatus
JPH11513887A (ja) * 1995-10-27 1999-11-30 ランバーグ，エリオット，アール． 特定のヌクレオチド配列の検出方法および組成物
US5727498A (en) * 1996-02-13 1998-03-17 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Retractable visual indicator assembly
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6426335B1 (en) 1997-10-17 2002-07-30 Gilead Sciences, Inc. Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6051698A (en) 1997-06-06 2000-04-18 Janjic; Nebojsa Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6235471B1 (en) * 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
AU1252099A (en) 1997-11-26 1999-06-15 Medical Research Council Improved selex procedure and an anti-cd4 aptamer
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
EP1049803A4 (fr) 1997-12-15 2002-08-21 Nexstar Pharmaceuticals Inc Detection homogene de molecules cibles par interaction ligand acide nucleique et ligand balise
US6175001B1 (en) * 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
US20030054360A1 (en) 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
US6329145B1 (en) * 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
EP1347301A1 (fr) * 2000-12-01 2003-09-24 International Reagents Corporation Procede de mesure ultrarapide et ultrasensible
WO2003014369A1 (fr) 2001-08-09 2003-02-20 Somalogic, Inc. Ligands d'acides nucleiques a duplexes intramoleculaires
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
US7767803B2 (en) 2002-06-18 2010-08-03 Archemix Corp. Stabilized aptamers to PSMA and their use as prostate cancer therapeutics
DE10241938A1 (de) * 2002-09-10 2004-03-25 Noxxon Pharma Ag Verfahren zur Selektion von Nukleinsäureliganden
CA2407825A1 (fr) 2002-10-11 2004-04-11 Andrew J. Simmonds Marquage par la methode trap : nouvelle methode d'identification et de purification de complexes arn-proteine
JP2004307464A (ja) * 2002-10-23 2004-11-04 Sankyo Co Ltd 糖部ｓ型配座の新規人工核酸
GB0226374D0 (en) * 2002-11-12 2002-12-18 Isis Innovation Ligands
EP1587908A4 (fr) * 2003-01-09 2008-02-20 Invitrogen Corp Liberation et activation de cellules de complexes de polypeptides et d'acides nucleiques
US20040235053A1 (en) * 2003-03-28 2004-11-25 The Regents Of The University Of California Preparation and application of encoded bead aggregates in combinatorial chemistry
US7672786B2 (en) 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
JP2007502417A (ja) * 2003-08-11 2007-02-08 モノグラム バイオサイエンシズ，インコーポレーテッド 分子複合体の検出および特徴づけ
KR100540509B1 (ko) * 2004-01-07 2006-01-11 학교법인 포항공과대학교 수화젤레이터로 사용되는 2'-데옥시우리딘 유도체
US20050227225A1 (en) * 2004-04-07 2005-10-13 Roche Molecular Systems, Inc. Stabilization of biomolecules in samples
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
EP1863828A4 (fr) * 2005-03-07 2010-10-13 Archemix Corp Aptameres stabilises d'apsm et leur utilisation comme agents therapeutiques contre le cancer de la prostate
WO2007002119A1 (fr) * 2005-06-24 2007-01-04 Mag Aerospace Industries, Inc. Systemes et procedes pour soupape d'interface d'eaux grises
EP1910837B1 (fr) * 2005-07-01 2012-03-14 Arbor Vita Corporation Methodes et compositions de diagnostic et de traitement de la grippe
JP2009505106A (ja) * 2005-08-19 2009-02-05 ナノスフェアー インコーポレイテッド Ｄｎａ及び抗体を含むハイブリッド基板を調製するための方法及びその使用
AU2008209447B2 (en) * 2007-01-23 2013-01-17 Jostens, Inc. Method and system for creating customized output
CN101809167B (zh) * 2007-07-17 2015-04-01 私募蛋白质体公司 产生具有改良的解离速率的适配体的方法
JP2013220095A (ja) * 2012-04-12 2013-10-28 Toyohiro Fujita 苗床
JP2018078250A (ja) 2016-11-11 2018-05-17 株式会社ニューフレアテクノロジー マルチ荷電粒子ビーム描画装置

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6030776A (en) * 1990-06-11 2000-02-29 Nexstar Pharmaceuticals, Inc. Parallel SELEX
WO1992014843A1 (fr) * 1991-02-21 1992-09-03 Gilead Sciences, Inc. Aptamere specifique de biomolecules et procede de production
US5645985A (en) * 1991-11-26 1997-07-08 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US6458539B1 (en) * 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
US5945527A (en) * 1996-05-30 1999-08-31 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US6458543B1 (en) * 1997-12-15 2002-10-01 Somalogic, Incorporated Nucleic acid ligand diagnostic biochip
US20060105378A1 (en) * 1999-07-29 2006-05-18 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
US20050158780A1 (en) * 2000-10-16 2005-07-21 Gilead Sciences, Inc. Nucleic acid ligands to the prostate specific membrane antigen
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US20050130195A1 (en) * 2002-03-19 2005-06-16 Fujitsu Limited Functional molecule and process for producing the same
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
WO2007084886A2 (fr) * 2006-01-17 2007-07-26 Somalogic, Incorporated Analyses multiplexees d’echantillons test
WO2008078180A2 (fr) * 2006-12-22 2008-07-03 Archemix Corp. Matériaux et procédés pour la génération de transcrits comprenant des nucléotides modifiés

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
DAVIS JONATHAN H ET AL: "Isolation of high-affinity GTP aptamers from partially structured RNA libraries", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 3 SEP 2002,, vol. 99, no. 18, 3 September 2002 (2002-09-03), pages 11616 - 11621, XP002555102 *
DIDONATO, D. A.: "Dissertation. Part II. Synthesis and Evaluation of Modified Nucleotides for DNA Aptamer Selection.", DISSERTATION, 9 August 2006 (2006-08-09), Raleigh, North Carolina, USA, pages I-XI, 26 - 115, XP002564655, Retrieved from the Internet <URL:http://repository.lib.ncsu.edu/ir/bitstream/1840.16/3954/1/etd.pdf> [retrieved on 20100119] *
EATON B E ET AL: "Post SELEX combinatorial optimization of aptamers", BIOORGANIC & MEDICINAL CHEMISTRY, PERGAMON, GB, vol. 5, no. 6, 1 January 1997 (1997-01-01), pages 1087 - 1096, XP002985590, ISSN: 0968-0896, DOI: 10.1016/S0968-0896(97)00044-8 *
JAYASENA S D: "APTAMERS: AN EMERGING CLASS OF MOLECULES THAT RIVAL ANTIBODIES IN DIAGNOSTICS", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 45, no. 9, 1 September 1999 (1999-09-01), pages 1628 - 1650, XP008067440, ISSN: 0009-9147 *
JHAVERI S ET AL: "In vitro selection of phosphorothiolated aptamers", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON, ELSEVIER SCIENCE, GB, vol. 8, no. 17, 8 September 1998 (1998-09-08), pages 2285 - 2290, XP004138219, ISSN: 0960-894X *
JONATHAN D. VAUGHT ET AL: "Expanding the Chemistry of DNA for in Vitro Selection", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132, no. 12, 31 March 2010 (2010-03-31), pages 4141 - 4151, XP055047152, ISSN: 0002-7863, DOI: 10.1021/ja908035g *
JUEWEN LIU ET AL: "Functional Nucleic Acid Sensors", CHEMICAL REVIEWS, vol. 109, no. 5, 20 March 2009 (2009-03-20), pages 1948 - 1998, XP055068159, ISSN: 0009-2665, DOI: 10.1021/cr030183i *
KEEFE A D ET AL: "SELEX with modified nucleotides", CURRENT OPINION IN CHEMICAL BIOLOGY, CURRENT BIOLOGY LTD, LONDON, GB, vol. 12, no. 4, 1 August 2008 (2008-08-01), pages 448 - 456, XP024528065, ISSN: 1367-5931, [retrieved on 20080721], DOI: 10.1016/J.CBPA.2008.06.028 *
LARRY GOLD ET AL: "Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery", PLOS ONE, vol. 5, no. 12, 7 December 2010 (2010-12-07), pages e15004, XP055040606, DOI: 10.1371/journal.pone.0015004 *
LATHAM J A ET AL: "The application of a modified nucleotide in aptamer selection : novel thrombin aptamers containing 5-(1-pentynyl)-2'-deoxyuridine", NUCLEIC ACIDS RESEARCH SPECIAL PUBLICATION, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 22, no. 14, 1 January 1994 (1994-01-01), pages 2817 - 2822, XP002176163, ISSN: 0305-1048 *
SHOJI A ET AL: "Modified DNA aptamer that binds the (R)-Isomer of a Thalidomide Derivative with High Enantioselectivity", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, ACS PUBLICATIONS, US, vol. 129, no. 5, 11 January 2007 (2007-01-11), pages 1456 - 1464, XP002494789, ISSN: 0002-7863, DOI: 10.1021/JA067098N *
TARASOW T M ET AL: "Dressed for success: Realizing the catalytic potential of RNA", BIOPOLYMERS - NUCLEIC ACID SCIENCES SECTION 1998 JOHN WILEY AND SONS INC. US, vol. 48, no. 1, 1998, pages 29 - 37, XP002564656 *

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