CN1642976A - 一种突变的免疫球蛋白-结合蛋白 - Google Patents
一种突变的免疫球蛋白-结合蛋白 Download PDFInfo
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Abstract
Description
突变体 | k开启[105M-1s-1] | k关闭[10-3s-1] | K亲和性[1107M-1] | ΔΔG(相对于Zwt)[kcal/mol] | ΔΔG(相对于Z(F30A))[kcal/mol] |
Zwt | 1.5 | 3.7 | 4.0 | 0 | |
Z(N23T) | 2.7 | 3.9 | 7 | -0.3 | |
Z(F30A) | 1.9 | 4.17 | 4.5 | -0.1 | 0.0 |
Z(F30A,N6A) | 7 | 21 | 3.3 | 0.1 | 0.2 |
Z(F30A,N11S) | 1.6 | 4.9 | 3.2 | 0.1 | 0.2 |
Z(F30A,N21A) | 1 | 3.8 | 2.6 | 0.3 | 0.4 |
Z(F30A,N23T) | 2.1 | 3.75 | 5.6 | -0.2 | -0.1 |
Z(F30A,N28A) | 3.1 | 9.87 | 3.2 | 0.1 | 0.2 |
Z(F30A,N43E) | 1.3 | 5.1 | 2.6 | 0.3 | 0.4 |
Z(F30A,N52A) | 1.5 | 4.9 | 3 | 0.2 | 0.3 |
Z(F30A,N23T,N43E) | 0.8 | 3.8 | 2 | 0.4 | 0.5 |
质粒编号pAY | 由构建体表达的蛋白 |
86 | Z(N23T/K4G) |
87 | Z(N23T/K4G)-Cys |
88 | Z(N23T/N3A) |
89 | Z(N23T/N3A)-Cys |
90 | Z(N23T/N3A/N6D) |
91 | Z(N23T/N3A/N6D)-Cys |
92 | Z(N23T/K4G)二聚体-Cys |
93 | Z(N23T/N3A)二聚体-Cys |
94 | Z(N23T/N3A/N6D)二聚体-Cys |
95 | Z(N23T/K4G)三聚体-Cys |
96 | Z(N23T/N3A)三聚体-Cys |
97 | Z(:N23T/N3A/N6D)三聚体-Cys |
98 | Z(N23T/K4G)四聚体-Cys |
99 | Z(N23T/N3A)四聚体-Cys |
100 | Z(N23T/N3A/N6D)四聚体-Cys |
101 | Z(N23T/K4G)五聚体-Cys |
102 | Z(N23T/N3A)五聚体-Cys |
103 | Z(N23T/N3A/N6D)五聚体-Cys |
质粒编号pAY | 由构建体表达的蛋白 |
107 | Z(N23T/N3A)-Cys |
108 | Z(N23T/N3A/N6D)-Cys |
109 | Z(N23T/N3A)二聚体-Cys |
110 | Z(N23T/N3A/N6D)二聚体-Cys |
111 | Z(N23T/N3A)三聚体-Cys |
112 | Z(N23T/N3A/N6D)三聚体-Cys |
113 | Z(N23T/N3A)四聚体-Cys |
114 | Z(N23T/N3A/N6D)四聚体-Cys |
115 | Z(N23T/N3A)五聚体-Cys |
116 | Z(N23T/N3A/N6D)五聚体-Cys |
质粒no.pAY | 由构建体表达的蛋白 |
118 | E’-Z(N23T/N3A)-Cys |
119 | E’-Z(N23T/N3A/N6D)-Cys |
120 | E’-Z(N23T/N3A)二聚体-Cys |
121 | E’-Z(N23T/N3A/N6D)二聚体-Cys |
122 | E’-Z(N23T/N3A)三聚体-Cys |
123 | E’-Z(N23T/N3A/N6D)三聚体-Cys |
124 | E’-Z(N23T/N3A)四聚体-Cys |
125 | E’-Z(N23T/N3A/N6D)四聚体-Cys |
126 | E’-Z(N23T/N3A)五聚体-Cys |
127 | E’-Z(N23T/N3A/N6D)五聚体-Cys |
Claims (21)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0200943-9 | 2002-03-25 | ||
SE0200943A SE0200943D0 (sv) | 2002-03-25 | 2002-03-25 | Mutant protein |
SE02009439 | 2002-03-25 | ||
PCT/SE2003/000475 WO2003080655A1 (en) | 2002-03-25 | 2003-03-20 | A mutated immunoglobulin-binding protein |
Related Child Applications (5)
Application Number | Title | Priority Date | Filing Date |
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CN201110407855.2A Division CN102532284B (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104134586A Division CN102558318A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104078514A Division CN102516372A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104078321A Division CN102516371A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104134571A Division CN102558317A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
Publications (2)
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CN1642976A true CN1642976A (zh) | 2005-07-20 |
CN1642976B CN1642976B (zh) | 2012-02-22 |
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CN2011104134571A Pending CN102558317A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN201110407855.2A Expired - Lifetime CN102532284B (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104078514A Pending CN102516372A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104078321A Pending CN102516371A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104134586A Pending CN102558318A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN038067935A Expired - Lifetime CN1642976B (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
Family Applications Before (5)
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CN2011104134571A Pending CN102558317A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN201110407855.2A Expired - Lifetime CN102532284B (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104078514A Pending CN102516372A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104078321A Pending CN102516371A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
CN2011104134586A Pending CN102558318A (zh) | 2002-03-25 | 2003-03-20 | 一种突变的免疫球蛋白-结合蛋白 |
Country Status (13)
Country | Link |
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US (9) | US7834158B2 (zh) |
EP (3) | EP3249047B1 (zh) |
JP (1) | JP4391830B2 (zh) |
KR (4) | KR101067181B1 (zh) |
CN (6) | CN102558317A (zh) |
AT (1) | ATE431360T1 (zh) |
AU (1) | AU2003217119B2 (zh) |
CA (1) | CA2479896C (zh) |
DE (1) | DE60327606D1 (zh) |
DK (2) | DK1972689T3 (zh) |
ES (3) | ES2325362T5 (zh) |
SE (1) | SE0200943D0 (zh) |
WO (1) | WO2003080655A1 (zh) |
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