JP5772594B2 - 形質転換体およびその製造方法、ならびに乳酸の製造方法 - Google Patents

形質転換体およびその製造方法、ならびに乳酸の製造方法 Download PDF

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JP5772594B2
JP5772594B2 JP2011527683A JP2011527683A JP5772594B2 JP 5772594 B2 JP5772594 B2 JP 5772594B2 JP 2011527683 A JP2011527683 A JP 2011527683A JP 2011527683 A JP2011527683 A JP 2011527683A JP 5772594 B2 JP5772594 B2 JP 5772594B2
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lactic acid
transformant
gene
culture
producing
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JPWO2011021629A1 (ja
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太志 原
太志 原
英毅 東田
英毅 東田
祐子 浜
祐子 浜
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AGC Inc
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Asahi Glass Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2011527683A 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法 Active JP5772594B2 (ja)

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JP2011527683A JP5772594B2 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法

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JP2009192271 2009-08-21
JP2009192271 2009-08-21
PCT/JP2010/063888 WO2011021629A1 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法
JP2011527683A JP5772594B2 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法

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JPWO2011021629A1 JPWO2011021629A1 (ja) 2013-01-24
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US (1) US9284561B2 (enExample)
EP (1) EP2468860B1 (enExample)
JP (1) JP5772594B2 (enExample)
KR (1) KR20120051705A (enExample)
CN (1) CN102498209A (enExample)
IN (1) IN2012DN01521A (enExample)
WO (1) WO2011021629A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5929895B2 (ja) * 2011-02-21 2016-06-08 旭硝子株式会社 乳酸の製造方法及び発酵賦活剤
CN104583396A (zh) * 2012-08-24 2015-04-29 旭硝子株式会社 转化体及其制造方法、以及乳酸的制造方法
WO2014156998A1 (ja) * 2013-03-28 2014-10-02 旭硝子株式会社 化成品の製造方法および製造装置
RU2539092C1 (ru) * 2013-09-27 2015-01-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") РЕКОМБИНАНТНЫЙ ШТАММ ДРОЖЖЕЙ Schizosaccharomyces pombe - ПРОДУЦЕНТ МОЛОЧНОЙ КИСЛОТЫ
JP6515502B2 (ja) * 2013-11-22 2019-05-22 Ａｇｃ株式会社 形質転換体の製造方法、形質転換体、および単座組込み用ベクターキット
US10597662B2 (en) 2013-11-22 2020-03-24 Jmtc Enzyme Corporation Transformant and process for production thereof, and process for production of lactic acid
EP2977471A1 (en) * 2014-07-23 2016-01-27 PURAC Biochem BV Genetic modification of (S)-lactic acid producing thermophilic bacteria
JP2017205019A (ja) * 2014-09-19 2017-11-24 旭硝子株式会社 有機酸の製造方法
JP6620375B2 (ja) * 2014-10-10 2019-12-18 Ｊｍｔｃエンザイム株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
BR112018010384A2 (pt) 2015-11-24 2018-12-04 Cargill Inc levedura geneticamente modificada e processo para fabricar um produto de fermentação
EP3178313A1 (en) 2015-12-09 2017-06-14 Leibniz-Institut für Pflanzenbiochemie Gene and protein for the synthesis of oxidized zingiberene derivatives
RU2614233C1 (ru) * 2015-12-22 2017-03-23 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Трансформант дрожжей Schizosaccharomyces pombe, продуцирующий молочную кислоту (варианты), способ его получения (варианты), способ микробиологического синтеза молочной кислоты с использованием такого трансформанта
RU2652877C9 (ru) * 2016-12-21 2018-06-26 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Способ модификации дрожжей Schizosaccharomyces pombe с помощью Cre-lox системы бактериофага Р1, трансформант, полученный таким способом
RU2650669C1 (ru) * 2016-12-21 2018-04-16 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Штамм Schizosaccharomyces pombe - продуцент молочной кислоты
KR102140596B1 (ko) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
BR112021002245A2 (pt) * 2018-08-09 2021-05-04 Juno Therapeutics Inc métodos para avaliar ácidos nucleicos integrados
SG11202101204TA (en) 2018-08-09 2021-03-30 Juno Therapeutics Inc Processes for generating engineered cells and compositions thereof
CN109280652A (zh) * 2018-09-13 2019-01-29 四川自豪时代药业有限公司 一种外源高表达乳酸脱氢酶突变体的高密度发酵方法
KR102736070B1 (ko) * 2018-10-08 2024-12-02 에스케이이노베이션 주식회사 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
EP4025700A1 (en) 2019-09-05 2022-07-13 Leibniz-Institut für Pflanzenbiochemie (IPB) Nucleic acids, proteins and processes for producing piperine and other amides
KR20210041903A (ko) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210128742A (ko) 2020-04-17 2021-10-27 에스케이이노베이션 주식회사 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210158676A (ko) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 젖산 생산능이 증가된 재조합 내산성 효모
WO2023004336A1 (en) * 2021-07-22 2023-01-26 Archer Daniels Midland Company A genetically engineered yeast producing lactic acid
EP4692365A1 (en) 2024-08-08 2026-02-11 Leibniz-Institut für Pflanzenbiochemie (IPB) Stiftung des öffentlichen Rechts Genes, proteins, and processes for the synthesis of blue anthocyanins in eukaryotic cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2776085B2 (ja) 1990-09-14 1998-07-16 旭硝子株式会社 ベクター
DE69435058T2 (de) 1993-10-05 2008-04-30 Asahi Glass Co., Ltd. Multicloning-vektor, expressionsvektor und herstellung von fremdproteinen unter verwendung des expressionsvektors
JP3689920B2 (ja) 1993-10-05 2005-08-31 旭硝子株式会社 マルチクローニングベクター、発現ベクター、および異種蛋白質の生産
JP3730256B2 (ja) 1995-02-03 2005-12-21 旭硝子株式会社 分泌シグナル遺伝子およびそれを有する発現ベクター
JPH10234375A (ja) 1997-02-28 1998-09-08 Asahi Glass Co Ltd マルチクローニングベクター
IT1294728B1 (it) 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
US6130070A (en) 1997-10-31 2000-10-10 Asahi Glass Company Ltd. Induction promoter gene and secretory signal gene usable in Schizosaccharomyces pombe, expression vectors having the same, and use thereof
US20070031950A1 (en) 1998-09-11 2007-02-08 Winkler Aaron A Production of D-lactic acid with yeast
JP4305998B2 (ja) 1999-03-18 2009-07-29 旭硝子株式会社 シゾサッカロミセス・ポンベの形質転換方法
JP2001204464A (ja) 2000-01-27 2001-07-31 Toyota Motor Corp 乳酸の製造方法
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
WO2002101038A1 (fr) 2001-05-29 2002-12-19 Asahi Glass Company, Limited Procede de construction d'un hote et procede de production d'une proteine etrangere
US7405068B2 (en) * 2003-05-02 2008-07-29 Tate & Lyle Ingredients Americas, Inc. Pyruvate producing yeast strain
US20050112737A1 (en) 2003-11-20 2005-05-26 A. E. Staley Manufacturing Co. Lactic acid producing yeast
JP5248735B2 (ja) 2004-01-19 2013-07-31 旭硝子株式会社 酵母の形質転換方法
JP2006006271A (ja) * 2004-06-29 2006-01-12 Toyota Central Res & Dev Lab Inc 乳酸生産酵母および乳酸生産方法
WO2007015470A1 (ja) 2005-08-03 2007-02-08 Asahi Glass Company, Limited 酵母宿主、形質転換体および異種タンパク質の製造方法
JPWO2007026617A1 (ja) 2005-08-29 2009-03-05 旭硝子株式会社 発現ベクター、それを導入した形質転換体、および異種タンパク質の製造方法
WO2007063919A1 (ja) 2005-11-29 2007-06-07 Asahi Glass Company, Limited 染色体改変方法
JP5329055B2 (ja) 2006-07-24 2013-10-30 東レ株式会社 変異型ピルビン酸脱炭酸酵素５遺伝子を有する酵母及び乳酸の製造方法
CA2710359C (en) * 2007-12-23 2018-02-20 Gevo, Inc. Yeast organism producing isobutanol at a high yield

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Publication number Publication date
KR20120051705A (ko) 2012-05-22
CN102498209A (zh) 2012-06-13
US9284561B2 (en) 2016-03-15
US20120214214A1 (en) 2012-08-23
IN2012DN01521A (enExample) 2015-06-05
EP2468860A4 (en) 2013-04-03
JPWO2011021629A1 (ja) 2013-01-24
EP2468860B1 (en) 2015-11-25
EP2468860A1 (en) 2012-06-27
WO2011021629A1 (ja) 2011-02-24
EP2468860A9 (en) 2012-12-26

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