JP5772594B2 - 形質転換体およびその製造方法、ならびに乳酸の製造方法 - Google Patents

形質転換体およびその製造方法、ならびに乳酸の製造方法 Download PDF

Info

Publication number
JP5772594B2
JP5772594B2 JP2011527683A JP2011527683A JP5772594B2 JP 5772594 B2 JP5772594 B2 JP 5772594B2 JP 2011527683 A JP2011527683 A JP 2011527683A JP 2011527683 A JP2011527683 A JP 2011527683A JP 5772594 B2 JP5772594 B2 JP 5772594B2
Authority
JP
Japan
Prior art keywords
lactic acid
transformant
gene
culture
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2011527683A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2011021629A1 (ja
Inventor
太志 原
太志 原
英毅 東田
英毅 東田
祐子 浜
祐子 浜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AGC Inc
Original Assignee
Asahi Glass Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Glass Co Ltd filed Critical Asahi Glass Co Ltd
Priority to JP2011527683A priority Critical patent/JP5772594B2/ja
Publication of JPWO2011021629A1 publication Critical patent/JPWO2011021629A1/ja
Application granted granted Critical
Publication of JP5772594B2 publication Critical patent/JP5772594B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2011527683A 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法 Active JP5772594B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2011527683A JP5772594B2 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2009192271 2009-08-21
JP2009192271 2009-08-21
JP2011527683A JP5772594B2 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法
PCT/JP2010/063888 WO2011021629A1 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法

Publications (2)

Publication Number Publication Date
JPWO2011021629A1 JPWO2011021629A1 (ja) 2013-01-24
JP5772594B2 true JP5772594B2 (ja) 2015-09-02

Family

ID=43607080

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011527683A Active JP5772594B2 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法

Country Status (7)

Country Link
US (1) US9284561B2 (enExample)
EP (1) EP2468860B1 (enExample)
JP (1) JP5772594B2 (enExample)
KR (1) KR20120051705A (enExample)
CN (1) CN102498209A (enExample)
IN (1) IN2012DN01521A (enExample)
WO (1) WO2011021629A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103392004A (zh) * 2011-02-21 2013-11-13 旭硝子株式会社 乳酸的制造方法
CN104583396A (zh) * 2012-08-24 2015-04-29 旭硝子株式会社 转化体及其制造方法、以及乳酸的制造方法
CN105189762A (zh) * 2013-03-28 2015-12-23 旭硝子株式会社 化工产品的制造方法及制造装置
RU2539092C1 (ru) * 2013-09-27 2015-01-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") РЕКОМБИНАНТНЫЙ ШТАММ ДРОЖЖЕЙ Schizosaccharomyces pombe - ПРОДУЦЕНТ МОЛОЧНОЙ КИСЛОТЫ
JP6488666B2 (ja) * 2013-11-22 2019-03-27 Ａｇｃ株式会社 クローニングベクター、発現ベクターおよび形質転換体の製造方法
JP6499587B2 (ja) * 2013-11-22 2019-04-10 Ｊｍｔｃエンザイム株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
EP2977471A1 (en) * 2014-07-23 2016-01-27 PURAC Biochem BV Genetic modification of (S)-lactic acid producing thermophilic bacteria
JP2017205019A (ja) * 2014-09-19 2017-11-24 旭硝子株式会社 有機酸の製造方法
US10214754B2 (en) * 2014-10-10 2019-02-26 Jmtc Enzyme Corporation Transformant and its production process, and method for producing lactic acid
CA3004176A1 (en) 2015-11-24 2017-06-01 Cargill, Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
EP3178313A1 (en) 2015-12-09 2017-06-14 Leibniz-Institut für Pflanzenbiochemie Gene and protein for the synthesis of oxidized zingiberene derivatives
RU2614233C1 (ru) * 2015-12-22 2017-03-23 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Трансформант дрожжей Schizosaccharomyces pombe, продуцирующий молочную кислоту (варианты), способ его получения (варианты), способ микробиологического синтеза молочной кислоты с использованием такого трансформанта
RU2652877C9 (ru) * 2016-12-21 2018-06-26 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Способ модификации дрожжей Schizosaccharomyces pombe с помощью Cre-lox системы бактериофага Р1, трансформант, полученный таким способом
RU2650669C1 (ru) * 2016-12-21 2018-04-16 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Штамм Schizosaccharomyces pombe - продуцент молочной кислоты
KR102140596B1 (ko) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
EA202190469A1 (ru) * 2018-08-09 2021-06-28 Джуно Терапьютикс, Инк. Способы оценки интегрированных нуклеиновых кислот
CN109280652A (zh) * 2018-09-13 2019-01-29 四川自豪时代药业有限公司 一种外源高表达乳酸脱氢酶突变体的高密度发酵方法
KR102736070B1 (ko) 2018-10-08 2024-12-02 에스케이이노베이션 주식회사 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
EP4025700A1 (en) 2019-09-05 2022-07-13 Leibniz-Institut für Pflanzenbiochemie (IPB) Nucleic acids, proteins and processes for producing piperine and other amides
KR20210041903A (ko) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210128742A (ko) 2020-04-17 2021-10-27 에스케이이노베이션 주식회사 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210158676A (ko) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 젖산 생산능이 증가된 재조합 내산성 효모
WO2023004336A1 (en) * 2021-07-22 2023-01-26 Archer Daniels Midland Company A genetically engineered yeast producing lactic acid

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2776085B2 (ja) 1990-09-14 1998-07-16 旭硝子株式会社 ベクター
JP3689920B2 (ja) 1993-10-05 2005-08-31 旭硝子株式会社 マルチクローニングベクター、発現ベクター、および異種蛋白質の生産
DE69435058T2 (de) 1993-10-05 2008-04-30 Asahi Glass Co., Ltd. Multicloning-vektor, expressionsvektor und herstellung von fremdproteinen unter verwendung des expressionsvektors
EP0773296B1 (en) 1995-02-03 2006-09-13 Asahi Glass Company Ltd. Secretory signal gene and expression vector having the same
JPH10234375A (ja) 1997-02-28 1998-09-08 Asahi Glass Co Ltd マルチクローニングベクター
IT1294728B1 (it) 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
US6130070A (en) 1997-10-31 2000-10-10 Asahi Glass Company Ltd. Induction promoter gene and secretory signal gene usable in Schizosaccharomyces pombe, expression vectors having the same, and use thereof
US20070031950A1 (en) 1998-09-11 2007-02-08 Winkler Aaron A Production of D-lactic acid with yeast
JP4305998B2 (ja) 1999-03-18 2009-07-29 旭硝子株式会社 シゾサッカロミセス・ポンベの形質転換方法
JP2001204464A (ja) 2000-01-27 2001-07-31 Toyota Motor Corp 乳酸の製造方法
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
JP4211603B2 (ja) * 2001-05-29 2009-01-21 旭硝子株式会社 シゾサッカロマイセス・ポンベ（Ｓｃｈｉｚｏｓａｃｃｈａｒｏｍｙｃｅｓｐｏｍｂｅ）宿主の構築方法および異種タンパク質の製造方法
US7405068B2 (en) * 2003-05-02 2008-07-29 Tate & Lyle Ingredients Americas, Inc. Pyruvate producing yeast strain
US20050112737A1 (en) 2003-11-20 2005-05-26 A. E. Staley Manufacturing Co. Lactic acid producing yeast
JP5248735B2 (ja) 2004-01-19 2013-07-31 旭硝子株式会社 酵母の形質転換方法
JP2006006271A (ja) * 2004-06-29 2006-01-12 Toyota Central Res & Dev Lab Inc 乳酸生産酵母および乳酸生産方法
EP2182060B1 (en) 2005-08-03 2012-10-24 Asahi Glass Company, Limited Transformed yeast host cells and method of producing foreign protein
EP1930423A4 (en) 2005-08-29 2009-12-09 Asahi Glass Co Ltd EXPRESSION VECTOR, TRANSFORMANT WITH THE EXPRESSION VECTOR INTRODUCED THEREIN AND METHOD FOR THE PRODUCTION OF HETEROLOGICAL PROTEIN
JP5200542B2 (ja) * 2005-11-29 2013-06-05 旭硝子株式会社 染色体改変方法
JP5329055B2 (ja) 2006-07-24 2013-10-30 東レ株式会社 変異型ピルビン酸脱炭酸酵素５遺伝子を有する酵母及び乳酸の製造方法
JP2011507532A (ja) * 2007-12-23 2011-03-10 ジーヴォ，インコーポレイテッド 高収率でイソブタノールを産生する酵母生物

Also Published As

Publication number Publication date
JPWO2011021629A1 (ja) 2013-01-24
EP2468860A1 (en) 2012-06-27
EP2468860A4 (en) 2013-04-03
US9284561B2 (en) 2016-03-15
CN102498209A (zh) 2012-06-13
EP2468860B1 (en) 2015-11-25
EP2468860A9 (en) 2012-12-26
IN2012DN01521A (enExample) 2015-06-05
KR20120051705A (ko) 2012-05-22
US20120214214A1 (en) 2012-08-23
WO2011021629A1 (ja) 2011-02-24

Similar Documents

Publication Publication Date Title
JP5772594B2 (ja) 形質転換体およびその製造方法、ならびに乳酸の製造方法
US9428777B2 (en) Transformant and method for producing same, and method for producing lactic acid
US9617570B2 (en) Acid resistant yeast cell and use thereof
JP2000509988A (ja) キシロースをエタノールに発酵するための安定な組換え酵母
JP2021058184A (ja) 乳酸代謝及びアルコール生成の抑制された組み換え耐酸性酵母及びこれを用いた乳酸の製造方法
JP6620375B2 (ja) 形質転換体およびその製造方法、ならびに乳酸の製造方法
JP2018157814A (ja) シュードザイマ・アンタクティカの新規菌株
CN101287833A (zh) 酵母和l-乳酸的制造方法
US9309542B2 (en) Recombinant Caldicellulosiruptor bescii and methods of use
JP6499587B2 (ja) 形質転換体およびその製造方法、ならびに乳酸の製造方法
JP6488666B2 (ja) クローニングベクター、発現ベクターおよび形質転換体の製造方法
JP6620373B2 (ja) 形質転換体およびその製造方法、ならびに炭素数４のジカルボン酸の製造方法
JP5565992B2 (ja) 酵母キャンディダ・ユティリスの形質転換法
JP2018011511A (ja) ３−ヒドロキシプロピオン酸の製造方法、および形質転換体

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130305

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140722

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140918

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20140919

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140930

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141020

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20141021

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20141020

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150310

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150507

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20150508

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150602

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150615

R150 Certificate of patent or registration of utility model

Ref document number: 5772594

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250