JP2000509988A - キシロースをエタノールに発酵するための安定な組換え酵母 - Google Patents
キシロースをエタノールに発酵するための安定な組換え酵母Info
- Publication number
- JP2000509988A JP2000509988A JP09540153A JP54015397A JP2000509988A JP 2000509988 A JP2000509988 A JP 2000509988A JP 09540153 A JP09540153 A JP 09540153A JP 54015397 A JP54015397 A JP 54015397A JP 2000509988 A JP2000509988 A JP 2000509988A
- Authority
- JP
- Japan
- Prior art keywords
- yeast
- xylose
- dna
- cells
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. キシロースリダクターゼ、キシリトールデヒドロゲナーゼ及びキシルロキ ナーゼをコードする遺伝子が、酵母の複数反復リボゾームDNA部位の各々に組 込まれた酵母を含む、キシロースをエタノールに発酵する酵母。 2. さらにグルコースのエタノールへの発酵も行なう請求項1記載の酵母。 3. Saccharomycesである請求項2記載の酵母。 4. 前記部位が非転写DNA部位である請求項3記載の酵母。 5. 前記遺伝子が、非グルコース抑制プロモーターに融合されており、酵母が グルコースとキシロースのエタノールへの発酵を同時に行なう請求項1記載の酵 母。 6. 前記プロモーターが誘導にキシロースを要求しない請求項5記載の酵母。 7. 前記遺伝子が、非グルコース抑制プロモーターに融合されており、酵母が グルコースとキシロースのエタノールへの発酵を同時に行なう請求項3記載の酵 母。 8. 前記遺伝子が、非グルコース抑制プロモーターに融合されており、酵母が グルコースとキシロースのエタノールへの発酵を同時に行ない、該プロモーター が誘導にキシロースを要求しない請求項4記載の酵母。 9.前記キシロースリダクターゼ及びキシリトールデヒドロゲナーゼ遺伝子が、 キシロースをエタノールに発酵する天然酵母由来である請求項6記載の酵母。 10. 前記天然酵母が、Candida Shehatae,Pichia stipitisまたはPachysolen tannophilusである請求項9の酵母。 11. 前記キシルロキナーゼ遺伝子が酵母または細菌由来である請求項9記載 の酵母。 12. 前記キシルロキナーゼ遺伝子が、Candida Shehatae,Pichiastipitis、P achysolen tannophilus、Saccharomyces cerevisiae、Schizosaccaromyces pomb eまたはEscherichia coli由来である請求項11記載の酵母。 13. 酵母の少なくとも約10個のリボソームDNA部位に組込まれた前記遺 伝子を有する請求項1記載の酵母。 14. (a)第1の選択マーカーを含む外来DNAを有する複製及び組込みプ ラスミドで細胞を形質転換し、そして (b)工程(a)で得られた細胞を繰り返し複製し、選択マーカー含む細胞を選 択しながら複数の世代の子孫細胞を生じさせ、その結果、子孫細胞の次の世代に おける複製及び組込プラスミドの保持を促進し、外来DNAの複数の挿入コピー を有する子孫細胞を生じさせることを含む方法。 15. 前記プラスミドDNAが、プラスミドを含む細胞を選択するための第二 の選択マーカーをも含む請求項14記載の方法。 16. 細胞が酵母または真核細胞であり、さらに、工程(b)で得られた子孫 細胞を、選択マーカーを含む細胞の選択をせずに繰り返し複製して、複数の世代 の子孫細胞を生じさせ、その結果次の世代の子孫細胞におけるプラスミドの喪失 を促進し、外来DNAの複数のコピーが各々染色体DNAに組込まれた酵母細胞 を回収することを含む請求項14記載の方法。 17. 前記細胞が酵母細胞であり、前記外来のDNAが、第一の選択マーカー としても機能するキシロースリダクターゼ、キシリトールデヒドロゲナーゼ、及 びキシルロキナーゼをコードする遺伝子を含む請求項16記載の方法。 18. (i)酵母細胞を選択マーカーを含む外来DNAを有する複製プラスミ ドで形質転換し、該外来DNAが、宿主のDNAの反復配列に相同なDNA配列 により各末端にフランキングしており、 (ii)工程(i)で得られた形質転換された酵母細胞を繰り返し複製し、選択マ ーカーを含む細胞を選択しながら複数の世代の子孫細胞を生じさせ、その結果、 子孫細胞の次の世代における複製プラスミドの保持を促進し、外来DNAの複数 の挿入コピーを有する子孫細胞を生じさせ、そして (iii)工程(ii)からの子孫細胞を、選択マーカーを含む細胞の選択をせずに 複製して、複数の世代の子孫細胞を製造し、その結果、次の世代の子孫細胞にお けるプラスミドの喪失を促進し、外来DNAの複数のコピーが各々染色体DNA に組込まれた酵母細胞を回収することを含む請求項14記載の方法。 19. 請求項18記載の方法により生産される酵母細胞。 20. 前記外来DNAが、キシロースリダクターゼ、キシリトールデヒドロゲ ナーゼ、及びキシルロキナーゼをコードする遺伝子を含み、前記酵母細胞が、キ シロースをエタノールに発酵する請求項19記載の酵母細胞。 21. 前記遺伝子が、誘導にキシロースを要求しない非グルコース抑制プロモ ーターに融合しており、前記酵母細胞が、グルコースとキシロースを同時にエタ ノールに発酵する請求項20記載の酵母細胞。 22. 非選択性条件で培養した場合に、キシロースをエタノールに発酵する能 力を、少なくとも20世代の間実質的に維持する請求項21記載の酵母細胞。 23. キシロースをエタノールに発酵する酵母であって、 該酵母の染色体DNAに組み込まれた外来DNAの複数のコピーを有し、前記 外来DNAが、非グルコース抑制プロモーターに融合したキシロースリダクター ゼ、キシリトールデヒドロゲナーゼ、及びキシルロキナーゼをコードする遺伝子 を含み、前記酵母が、グルコースとキシロースを同時にエタノールに発酵し、そ して非選択性条件で培養した場合に、キシロースをエタノールに発酵する能力を 、少なくとも20世代の間実質的に維持する酵母を含む酵母。 24. 前記プロモーターが誘導にキシロースを要求しない請求項23記載の酵 母。 25. キシロースをエタノールに発酵する酵母であって、 キシロースリダクターゼ、キシリトールデヒドロゲナーゼ、及びキシルロキナ ーゼをコードする導入されたDNAの複数のコピーを含み、前記酵母がキシロー スをエタノールに発酵し、そして非選択性条件で培養した場合に、キシロースを エタノールに発酵する能力を、少なくとも20世代の間実質的に維持する酵母を 含む酵母。 26. 前記プロモーターが誘導にキシロースを要求しない請求項25記載の酵 母。 27. 請求項1、22、23、24、25または26記載の酵母を用いて、キ シロース含有培地を発酵してエタノールを生産することを含む、キシロースのエ タノールへの発酵方法。 28. 第一の選択マーカーを含む外来DNA配列を標的酵母細胞の染色体DN Aに組込むためのプラスミドベクターであって、機能的な酵母DNA複製起点と 、標的酵母細胞のリボソームDNAの反復配列に相同なDNAフランキング配列 に より各末端にフランキングする外来DNAを含み、該プラスミドは、さらに第二 の選択マーカーをDNAフランキング配列の問以外の位置に有するプラスミドベ クター。 29. 外来DNA配列を酵母に組み込んで、キシロースをエタノールに発酵す る安定な組込み体を形成するためのプラスミドベクターであって、機能的な酵母 DNA複製起点と、標的酵母細胞の反復DNA配列に相同なDNAフランキング 配列により各末端にフランキングするキシロースリダクターゼ、キシリトールデ ヒドロゲナーゼ、及びキシルロキナーゼをコードする遺伝子含む外来DNAを含 むプラスミドベクター。 30. 外来DNA断片のコピーが複数組み込まれた細胞を形成するための方法 であって、反復ゲノムDNAを有し、外来DNAを含む複製及び組込プラスミド を含む細胞を複製し、選択マーカーを含む細胞を選択しながら複数の世代の子孫 細胞を生じさせ、その結果、子孫細胞の次の世代における複製及び組込みプラス ミドの保持を促進し、外来DNAの複数の組込コピーを有する子孫細胞を生じさ せることを含む方法。
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011083255A (ja) * | 2009-10-19 | 2011-04-28 | Kumamoto Univ | キシロースからエタノールを発酵する酵母 |
JP2012115248A (ja) * | 2010-12-03 | 2012-06-21 | National Institute Of Advanced Industrial Science & Technology | キシロース代謝改変による効果的なエタノール生産法 |
KR20180000795A (ko) * | 2016-06-23 | 2018-01-04 | 안동대학교 산학협력단 | 마를 포함하는 알코올 발효 촉진용 배지 조성물 및 이를 이용한 마 발효주 |
Families Citing this family (77)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IN191596B (ja) * | 1996-05-06 | 2003-12-06 | Purdue Research Foundation | |
FI980551A (fi) * | 1998-03-11 | 1999-09-12 | Valtion Teknillinen | Transformoidut mikro-organismit, joilla on parannettuja ominaisuuksia |
EP1071786B1 (en) | 1998-04-20 | 2005-11-30 | Forskarpatent i Syd AB | Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates |
EP1278876A2 (en) * | 2000-05-01 | 2003-01-29 | Midwest Research Institute | STABLE i ZYMOMONAS MOBILIS /i XYLOSE AND ARABINOSE FERMENTING STRAINS |
US7354755B2 (en) | 2000-05-01 | 2008-04-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
WO2001088094A1 (en) * | 2000-05-15 | 2001-11-22 | Forskarpatent I Syd | A recombinant yeast for lignocellulose raw materials |
EP1364072B1 (en) | 2001-02-28 | 2007-01-03 | Iogen Energy Corporation | Method of processing lignocellulosic feedstock for enhanced xylose and ethanol production |
PL1626979T3 (pl) | 2003-05-02 | 2012-09-28 | Cargill Inc | Genetycznie modyfikowane gatunki drożdży i sposoby fermentacji z użyciem genetycznie modyfikowanych drożdży |
CN100339483C (zh) * | 2003-12-19 | 2007-09-26 | 首都师范大学 | 酵母菌利用木糖和葡萄糖生产酒精的方法 |
ATE546534T1 (de) * | 2005-03-02 | 2012-03-15 | Pharmedartis Gmbh | Expression system für rekombinante proteine |
US20150328347A1 (en) | 2005-03-24 | 2015-11-19 | Xyleco, Inc. | Fibrous materials and composites |
CN1325633C (zh) * | 2005-08-08 | 2007-07-11 | 天津大学 | 甘油通道蛋白基因缺失降低甘油生成提高乙醇产量的酿酒酵母菌株及构建方法 |
BRPI0713506A2 (pt) | 2006-06-22 | 2012-03-13 | Iogen Energy Corporation | composiçõpes de enzima para a hidrólise enzimática aperfeiçoada de celulose e método de uso das mesmas |
CN1966694B (zh) * | 2006-11-15 | 2010-05-12 | 山东大学 | 一种利用葡萄糖木糖共发酵生产酒精的方法 |
ATE506447T1 (de) * | 2007-10-30 | 2011-05-15 | Du Pont | Verfahren zur herstellung von etahnol in einem xylose enthaltenden medium unter verwendung eines rekombinanten zymomonas stammes mit reduzierter hima expression |
CN101463328B (zh) * | 2007-12-19 | 2011-06-29 | 中国科学院微生物研究所 | 一株利用木糖发酵产乙醇的代谢工程酵母菌 |
US9228178B2 (en) | 2008-05-30 | 2016-01-05 | Archer Daniels Midland Co. | Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha |
CN101302535B (zh) * | 2008-06-30 | 2010-10-20 | 黑龙江大学 | 一种表达木酮糖激酶基因的质粒的构建方法 |
US9234413B2 (en) | 2009-06-25 | 2016-01-12 | Shell Oil Company | Water injection systems and methods |
US20120184007A1 (en) | 2009-07-09 | 2012-07-19 | Stephen Picataggio | Engineered microorganisms with enhanced fermentation activity |
CA2719280A1 (en) | 2009-10-28 | 2011-04-28 | Erin Johnson | Novel ethanol-producing yeast |
CA2779163A1 (en) | 2009-12-30 | 2011-07-07 | Iogen Energy Corporation | Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates |
US8759049B2 (en) | 2010-02-25 | 2014-06-24 | Iogen Energy Corporation | Method for the production of a fermentation product from a sugar hydrolysate |
CN102220254B (zh) * | 2010-04-14 | 2013-04-17 | 新疆农业科学院生物质能源研究所 | 一种重组酿酒酵母工程菌株及其应用 |
JP2013524797A (ja) | 2010-04-21 | 2013-06-20 | ディーエスエム アイピー アセッツ ビー.ブイ. | アラビノースを転化できる細胞を製造する方法 |
US9493802B2 (en) | 2010-08-20 | 2016-11-15 | Codexis, Inc. | Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose |
US8709770B2 (en) | 2010-08-31 | 2014-04-29 | Iogen Energy Corporation | Process for improving the hydrolysis of cellulose in high consistency systems using one or more unmixed and mixed hydrolysis reactors |
WO2012045088A2 (en) * | 2010-10-01 | 2012-04-05 | The Board Of Trustees Of The University Of Illinois | Combinatorial design of highly efficient heterologous pathways |
US8889384B2 (en) | 2010-10-07 | 2014-11-18 | Shell Oil Company | Process for the production of alcohols from biomass |
US9290821B2 (en) | 2010-11-05 | 2016-03-22 | Shell Oil Company | Treating biomass to produce materials useful for biofuels |
KR101204366B1 (ko) * | 2010-11-16 | 2012-11-27 | 서울대학교산학협력단 | 토르(Tor) 신호 전달 경로에 관여하는 유전자의 기능을 소실시킨 재조합 사카로마이세스 세레비지애를 이용하여 자일로오스로부터 에탄올을 생산하는 방법 |
WO2012071470A2 (en) | 2010-11-22 | 2012-05-31 | Cargill, Incorporated | Compositions and methods for increased ethanol titer from biomass |
JP2012120491A (ja) * | 2010-12-09 | 2012-06-28 | Toyota Motor Corp | キシロースを含む培地における発酵培養方法 |
WO2012088108A1 (en) | 2010-12-20 | 2012-06-28 | Shell Oil Company | Process for the production of alcohols from biomass |
US8728798B2 (en) | 2011-05-03 | 2014-05-20 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
TWI438274B (zh) * | 2011-06-03 | 2014-05-21 | Inst Nuclear Energy Res Atomic Energy Council | 一種木糖代謝菌之製備方法及該木糖代謝菌 |
BR112014004171A2 (pt) | 2011-08-22 | 2018-11-06 | Codexis Inc | variantes da proteína glicósideo hidrolase gh61 e cofatores que aumentam a atividade de gh61 |
BR112014007719A2 (pt) | 2011-09-30 | 2017-06-13 | Codexis Inc | proteases fúngicas |
BR112014013854B1 (pt) | 2011-12-15 | 2021-07-13 | Shell Internationale Research Maatschappij B.V. | Método para o tratamento de um subproduto da produção de etanol |
WO2013169706A1 (en) | 2012-05-07 | 2013-11-14 | Shell Oil Company | Continuous or semi-continuous process for treating biomass to produce materials useful for biofuels |
CA2873298C (en) | 2012-05-17 | 2016-04-26 | Corey William RADTKE | Process for producing volatile organic compounds from biomass material |
JP6291482B2 (ja) | 2012-05-17 | 2018-03-14 | シエル・インターナシヨネイル・リサーチ・マーチヤツピイ・ベー・ウイShell Internationale Research Maatschappij Besloten Vennootshap | バイオマス材料を処理する方法およびシステム |
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Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4009676A1 (de) | 1990-03-26 | 1991-10-02 | Rhein Biotech Proz & Prod Gmbh | Dna-sequenz, umfassend ein fuer xylosereduktase und/oder xylitoldehydrogenase codierendes strukturgen |
US5866382A (en) * | 1990-04-06 | 1999-02-02 | Xyrofin Oy | Xylose utilization by recombinant yeasts |
FI901771A (fi) | 1990-04-06 | 1991-10-07 | Valtion Teknillinen | Anvaendning av xylos hos hybridjaest. |
US5789210A (en) * | 1993-11-08 | 1998-08-04 | Purdue Research Foundation | Recombinant yeasts for effective fermentation of glucose and xylose |
IN191596B (ja) * | 1996-05-06 | 2003-12-06 | Purdue Research Foundation |
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- 1997-05-06 EP EP97922698A patent/EP0898616A4/en not_active Withdrawn
- 1997-05-06 CA CA2661090A patent/CA2661090C/en not_active Expired - Fee Related
- 1997-05-06 US US09/180,340 patent/US7527927B1/en not_active Expired - Fee Related
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- 1997-05-06 WO PCT/US1997/007663 patent/WO1997042307A1/en not_active Application Discontinuation
- 1997-05-06 ID IDP971509A patent/ID16873A/id unknown
- 1997-05-06 CN CNB971961956A patent/CN1238490C/zh not_active Expired - Lifetime
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- 1997-05-06 JP JP54015397A patent/JP4321878B2/ja not_active Expired - Fee Related
- 1997-06-05 UA UA98126403A patent/UA76690C2/uk unknown
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Cited By (4)
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JP2011083255A (ja) * | 2009-10-19 | 2011-04-28 | Kumamoto Univ | キシロースからエタノールを発酵する酵母 |
JP2012115248A (ja) * | 2010-12-03 | 2012-06-21 | National Institute Of Advanced Industrial Science & Technology | キシロース代謝改変による効果的なエタノール生産法 |
KR20180000795A (ko) * | 2016-06-23 | 2018-01-04 | 안동대학교 산학협력단 | 마를 포함하는 알코올 발효 촉진용 배지 조성물 및 이를 이용한 마 발효주 |
KR102062144B1 (ko) | 2016-06-23 | 2020-01-03 | 안동대학교 산학협력단 | 마를 포함하는 알코올 발효 촉진용 배지 조성물 및 이를 이용한 마 발효주 |
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WO1997042307A1 (en) | 1997-11-13 |
CA2253581A1 (en) | 1997-11-13 |
EP0898616A4 (en) | 2000-12-06 |
US8652772B2 (en) | 2014-02-18 |
AU731102B2 (en) | 2001-03-22 |
JP4321878B2 (ja) | 2009-08-26 |
EP0898616A1 (en) | 1999-03-03 |
CA2253581C (en) | 2012-04-17 |
ID16873A (id) | 1997-11-20 |
JP2009148277A (ja) | 2009-07-09 |
CN1238490C (zh) | 2006-01-25 |
UA76690C2 (uk) | 2006-09-15 |
US20090246857A1 (en) | 2009-10-01 |
US7527927B1 (en) | 2009-05-05 |
AU2830197A (en) | 1997-11-26 |
CA2661090A1 (en) | 1997-11-13 |
CA2661090C (en) | 2013-02-05 |
CN1225125A (zh) | 1999-08-04 |
IN191596B (ja) | 2003-12-06 |
BR9710963A (pt) | 2001-07-31 |
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