BRPI0713506A2 - composiçõpes de enzima para a hidrólise enzimática aperfeiçoada de celulose e método de uso das mesmas - Google Patents

composiçõpes de enzima para a hidrólise enzimática aperfeiçoada de celulose e método de uso das mesmas Download PDF

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Publication number
BRPI0713506A2
BRPI0713506A2 BRPI0713506-8A BRPI0713506A BRPI0713506A2 BR PI0713506 A2 BRPI0713506 A2 BR PI0713506A2 BR PI0713506 A BRPI0713506 A BR PI0713506A BR PI0713506 A2 BRPI0713506 A2 BR PI0713506A2
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Brazil
Prior art keywords
enzyme
hydrolysis
enzyme compositions
glycosidase
cellulose hydrolysis
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Application number
BRPI0713506-8A
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English (en)
Inventor
S. Tolan Jeffrey
White Theresa
Tomashek John
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Iogen Energy Corporation
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Publication date
Application filed by Iogen Energy Corporation filed Critical Iogen Energy Corporation
Publication of BRPI0713506A2 publication Critical patent/BRPI0713506A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

COMPOSIçõES DE ENZIMA PARA A HIDRóLISE ENZIMáTICA APERFEIçOADA DE CELULOSE E MéTODO DE USO DAS MESMAS. A presente invenção refere-se a um processo para a hidrólise enzimática de celulose para produzir um produto de hidrólise compreendendo glicose a partir de um estoque de alimentação lignocelulósico pré-tratado e enzimas para uso no processo O processo compreende hidrolisação de uma pasta fluida aquosa de um estoque de alimentação lignocelulósico pré-tratado com enzimas celulase, uma ou mais do que uma enzima <225>-glicosidase e um agente de ligação para ligação da enzima <225>-glicosidase aos sólidos de fibra presentes na pasta fluida aquosa. Durante a hidrólise, ambas a enzima celulase e a enzima <225>-glicosidase se ligam aos sólidos de fibra. A hidrólise é realizada em um reator de hidrólise de retenção de sólidos de modo que os sólidos de fibra não-hidrolisados e enzima ligada são retidos no reator mais tempo do que a fase aquosa da pasta fluida.
BRPI0713506-8A 2006-06-22 2007-06-22 composiçõpes de enzima para a hidrólise enzimática aperfeiçoada de celulose e método de uso das mesmas BRPI0713506A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81581806P 2006-06-22 2006-06-22
US60/815,818 2006-06-22
PCT/CA2007/001133 WO2007147264A1 (en) 2006-06-22 2007-06-22 Enzyme compositions for the improved enzymatic hydrolysis of cellulose and methods of using same

Publications (1)

Publication Number Publication Date
BRPI0713506A2 true BRPI0713506A2 (pt) 2012-03-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0713506-8A BRPI0713506A2 (pt) 2006-06-22 2007-06-22 composiçõpes de enzima para a hidrólise enzimática aperfeiçoada de celulose e método de uso das mesmas

Country Status (7)

Country Link
US (1) US8318461B2 (pt)
EP (1) EP2029762A4 (pt)
CN (1) CN101501205A (pt)
AU (1) AU2007262634A1 (pt)
BR (1) BRPI0713506A2 (pt)
CA (1) CA2655645A1 (pt)
WO (1) WO2007147264A1 (pt)

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US20110039319A1 (en) * 2009-08-12 2011-02-17 Theodora Retsina Enzyme recycle from hydrolysis of lignocellulosic material
WO2011041594A1 (en) 2009-09-30 2011-04-07 Codexis, Inc. Recombinant c1 b-glucosidase for production of sugars from cellulosic biomass
DK2504017T3 (en) 2009-11-25 2014-03-03 Codexis Inc RECOMBINANT BETA-glucosidase OPTIONS FOR PRODUCTION OF soluble sugars FROM cellulosic biomass
EP2504431A4 (en) 2009-11-25 2013-10-16 Codexis Inc RECOMBINANT BETA-GLUCOSIDASE VARIANTS OF THERMOASCUS AURIENTIACUS FOR THE PRODUCTION OF FERMENTABLE SUGARS FROM CELLULOSIC BIOMASS
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WO2011150313A1 (en) 2010-05-28 2011-12-01 Codexis, Inc. Pentose fermentation by a recombinant microorganism
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US9175315B2 (en) 2010-06-18 2015-11-03 Butamax Advanced Biofuels Llc Production of alcohol esters and in situ product removal during alcohol fermentation
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Also Published As

Publication number Publication date
EP2029762A4 (en) 2010-04-28
CN101501205A (zh) 2009-08-05
US8318461B2 (en) 2012-11-27
AU2007262634A1 (en) 2007-12-27
EP2029762A1 (en) 2009-03-04
CA2655645A1 (en) 2007-12-27
US20090209009A1 (en) 2009-08-20
WO2007147264A1 (en) 2007-12-27
WO2007147264A8 (en) 2008-03-20

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2230 DE 01/10/2013.