CN102498209A - 转化体及其制造方法以及乳酸的制造方法 - Google Patents

转化体及其制造方法以及乳酸的制造方法 Download PDF

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Publication number
CN102498209A
CN102498209A CN2010800371957A CN201080037195A CN102498209A CN 102498209 A CN102498209 A CN 102498209A CN 2010800371957 A CN2010800371957 A CN 2010800371957A CN 201080037195 A CN201080037195 A CN 201080037195A CN 102498209 A CN102498209 A CN 102498209A
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Prior art keywords
transformant
lactic acid
gene
schizosaccharomyces pombe
host
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CN2010800371957A
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English (en)
Chinese (zh)
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原太志
东田英毅
浜祐子（已逝）
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AGC Inc
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Asahi Glass Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2010800371957A 2009-08-21 2010-08-17 转化体及其制造方法以及乳酸的制造方法 Pending CN102498209A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009192271 2009-08-21
JP2009-192271 2009-08-21
PCT/JP2010/063888 WO2011021629A1 (ja) 2009-08-21 2010-08-17 形質転換体およびその製造方法、ならびに乳酸の製造方法

Publications (1)

Publication Number Publication Date
CN102498209A true CN102498209A (zh) 2012-06-13

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CN2010800371957A Pending CN102498209A (zh) 2009-08-21 2010-08-17 转化体及其制造方法以及乳酸的制造方法

Country Status (7)

Country Link
US (1) US9284561B2 (enExample)
EP (1) EP2468860B1 (enExample)
JP (1) JP5772594B2 (enExample)
KR (1) KR20120051705A (enExample)
CN (1) CN102498209A (enExample)
IN (1) IN2012DN01521A (enExample)
WO (1) WO2011021629A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104583396A (zh) * 2012-08-24 2015-04-29 旭硝子株式会社 转化体及其制造方法、以及乳酸的制造方法
CN105189762A (zh) * 2013-03-28 2015-12-23 旭硝子株式会社 化工产品的制造方法及制造装置
CN106536543A (zh) * 2014-07-23 2017-03-22 普拉克生化公司 产生(s)‑乳酸的嗜热细菌的遗传修饰
CN109280652A (zh) * 2018-09-13 2019-01-29 四川自豪时代药业有限公司 一种外源高表达乳酸脱氢酶突变体的高密度发酵方法

Families Citing this family (19)

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CN103392004A (zh) * 2011-02-21 2013-11-13 旭硝子株式会社 乳酸的制造方法
RU2539092C1 (ru) * 2013-09-27 2015-01-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") РЕКОМБИНАНТНЫЙ ШТАММ ДРОЖЖЕЙ Schizosaccharomyces pombe - ПРОДУЦЕНТ МОЛОЧНОЙ КИСЛОТЫ
JP6488666B2 (ja) * 2013-11-22 2019-03-27 Ａｇｃ株式会社 クローニングベクター、発現ベクターおよび形質転換体の製造方法
JP6499587B2 (ja) * 2013-11-22 2019-04-10 Ｊｍｔｃエンザイム株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
JP2017205019A (ja) * 2014-09-19 2017-11-24 旭硝子株式会社 有機酸の製造方法
US10214754B2 (en) * 2014-10-10 2019-02-26 Jmtc Enzyme Corporation Transformant and its production process, and method for producing lactic acid
CA3004176A1 (en) 2015-11-24 2017-06-01 Cargill, Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
EP3178313A1 (en) 2015-12-09 2017-06-14 Leibniz-Institut für Pflanzenbiochemie Gene and protein for the synthesis of oxidized zingiberene derivatives
RU2614233C1 (ru) * 2015-12-22 2017-03-23 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Трансформант дрожжей Schizosaccharomyces pombe, продуцирующий молочную кислоту (варианты), способ его получения (варианты), способ микробиологического синтеза молочной кислоты с использованием такого трансформанта
RU2652877C9 (ru) * 2016-12-21 2018-06-26 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Способ модификации дрожжей Schizosaccharomyces pombe с помощью Cre-lox системы бактериофага Р1, трансформант, полученный таким способом
RU2650669C1 (ru) * 2016-12-21 2018-04-16 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Штамм Schizosaccharomyces pombe - продуцент молочной кислоты
KR102140596B1 (ko) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
EA202190469A1 (ru) * 2018-08-09 2021-06-28 Джуно Терапьютикс, Инк. Способы оценки интегрированных нуклеиновых кислот
KR102736070B1 (ko) 2018-10-08 2024-12-02 에스케이이노베이션 주식회사 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
EP4025700A1 (en) 2019-09-05 2022-07-13 Leibniz-Institut für Pflanzenbiochemie (IPB) Nucleic acids, proteins and processes for producing piperine and other amides
KR20210041903A (ko) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 락테이트 대사 및 알코올 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210128742A (ko) 2020-04-17 2021-10-27 에스케이이노베이션 주식회사 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
KR20210158676A (ko) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 젖산 생산능이 증가된 재조합 내산성 효모
WO2023004336A1 (en) * 2021-07-22 2023-01-26 Archer Daniels Midland Company A genetically engineered yeast producing lactic acid

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104583396A (zh) * 2012-08-24 2015-04-29 旭硝子株式会社 转化体及其制造方法、以及乳酸的制造方法
CN105189762A (zh) * 2013-03-28 2015-12-23 旭硝子株式会社 化工产品的制造方法及制造装置
CN106536543A (zh) * 2014-07-23 2017-03-22 普拉克生化公司 产生(s)‑乳酸的嗜热细菌的遗传修饰
CN106536543B (zh) * 2014-07-23 2021-05-07 普拉克生化公司 产生(s)-乳酸的嗜热细菌的遗传修饰
CN109280652A (zh) * 2018-09-13 2019-01-29 四川自豪时代药业有限公司 一种外源高表达乳酸脱氢酶突变体的高密度发酵方法

Also Published As

Publication number Publication date
JPWO2011021629A1 (ja) 2013-01-24
EP2468860A1 (en) 2012-06-27
EP2468860A4 (en) 2013-04-03
US9284561B2 (en) 2016-03-15
EP2468860B1 (en) 2015-11-25
JP5772594B2 (ja) 2015-09-02
EP2468860A9 (en) 2012-12-26
IN2012DN01521A (enExample) 2015-06-05
KR20120051705A (ko) 2012-05-22
US20120214214A1 (en) 2012-08-23
WO2011021629A1 (ja) 2011-02-24

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