JP2018503388A - 組み換え宿主におけるステビオールグリコシドの生産 - Google Patents

組み換え宿主におけるステビオールグリコシドの生産 Download PDF

Info

Publication number
JP2018503388A
JP2018503388A JP2017540261A JP2017540261A JP2018503388A JP 2018503388 A JP2018503388 A JP 2018503388A JP 2017540261 A JP2017540261 A JP 2017540261A JP 2017540261 A JP2017540261 A JP 2017540261A JP 2018503388 A JP2018503388 A JP 2018503388A
Authority
JP
Japan
Prior art keywords
seq
steviol
polypeptide
cell
ent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017540261A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018503388A5 (https=
Inventor
オルソン，キム
シモン，エルネスト
ミッケルセン，ミカエル ダルガート
ミッケルセン，ミカエル ダルガート
カールセン，サイモン
ドーチン，ヴェロニク
チャン リム，スウィー
チャン リム，スウィー
ダリン，ルイス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evolva Holding SA
Original Assignee
Evolva AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva AG filed Critical Evolva AG
Publication of JP2018503388A publication Critical patent/JP2018503388A/ja
Publication of JP2018503388A5 publication Critical patent/JP2018503388A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Seasonings (AREA)
JP2017540261A 2015-01-30 2016-01-29 組み換え宿主におけるステビオールグリコシドの生産 Pending JP2018503388A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562110207P 2015-01-30 2015-01-30
US62/110,207 2015-01-30
PCT/EP2016/052007 WO2016120486A1 (en) 2015-01-30 2016-01-29 Production of steviol glycosides in recombinant hosts

Publications (2)

Publication Number Publication Date
JP2018503388A true JP2018503388A (ja) 2018-02-08
JP2018503388A5 JP2018503388A5 (https=) 2019-03-14

Family

ID=55398256

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017540261A Pending JP2018503388A (ja) 2015-01-30 2016-01-29 組み換え宿主におけるステビオールグリコシドの生産

Country Status (9)

Country Link
US (4) US10364450B2 (https=)
EP (2) EP4148137A1 (https=)
JP (1) JP2018503388A (https=)
CN (1) CN108337892B (https=)
AU (3) AU2016211124B2 (https=)
BR (1) BR112017016338A2 (https=)
CA (1) CA2973674A1 (https=)
SG (1) SG11201705606PA (https=)
WO (1) WO2016120486A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019517811A (ja) * 2016-06-17 2019-06-27 カーギル インコーポレイテッド 経口摂取のためのステビオール配糖体組成物または使用
JP2022513480A (ja) * 2018-12-12 2022-02-08 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
CN108396044A (zh) 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CA2900882A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinants hosts
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3232817A4 (en) * 2014-12-17 2018-10-10 Cargill, Incorporated Steviol glycoside compounds, compositions for oral ingestion or use, and method for enhancing steviol glycoside solubility
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
EP3718417B1 (en) * 2015-04-03 2025-09-03 DSM IP Assets B.V. Steviol glycosides
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201804542YA (en) * 2015-11-30 2018-06-28 Cargill Inc Steviol glycoside compositions for oral ingestion or use
US20190048356A1 (en) * 2016-03-11 2019-02-14 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) * 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
JP2019517812A (ja) * 2016-06-17 2019-06-27 カーギル インコーポレイテッド 経口摂取または経口使用のためのステビオールグリコシド組成物
AU2017308143B2 (en) 2016-08-12 2022-04-07 Amyris, Inc. UDP-dependent glycosyltransferase for high efficiency production of rebaudiosides
EP3522737B1 (en) 2016-10-04 2025-12-03 The Coca-Cola Company Diterpene glycosides containing an ent-atisene core, compositions and methods
CN110191643B (zh) * 2016-10-14 2023-06-06 科纳根公司 甜菊醇糖苷的生物合成生产及其工艺
AU2017345605B2 (en) * 2016-10-20 2022-04-14 The Coca-Cola Company Diterpene glycosides isolated from Stevia, compositions and methods
BR112019008048A2 (pt) 2016-10-21 2019-07-02 Pepsico Inc método para preparar rebaudiosídeo n com o uso de método enzimático
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
CA3175070A1 (en) 2017-02-03 2018-08-09 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
MX2019015404A (es) 2017-06-30 2020-07-20 Conagen Inc Hidrólisis de glucósidos de esteviol por beta-glucosidasa.
US11365417B2 (en) 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
JP2021518369A (ja) * 2018-03-16 2021-08-02 ピュアサークル ユーエスエー インコーポレイテッド 高純度ステビオール配糖体
AU2019282411B2 (en) * 2018-06-08 2024-09-19 Purecircle Usa Inc. High-purity steviol glycosides
WO2020028039A1 (en) 2018-07-30 2020-02-06 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
MX2021004192A (es) * 2018-10-15 2021-05-27 Amyris Inc Variantes de acido kaurenoico hidroxilasa de stevia rebaudiana para la produccion de rebaudiosidos de alta eficiencia.
CN113302298B (zh) 2018-11-09 2025-02-25 银杏生物制品公司 罗汉果甙的生物合成
BR112021010189A2 (pt) * 2018-11-27 2021-11-09 Purecircle Usa Inc Glicosídeos de esteviol i-xvi, métodos para produção de ao menos um glicosídeo de esteviol, para intensificação do dulçor de um produto alimentício ou de bebida, para modificação do sabor de um produto alimentício ou de bebida e para supressão da formação de espuma em um produto alimentício ou de bebida, e, produto consumível
MX2021009619A (es) * 2019-02-15 2021-11-04 Purecircle Usa Inc Glicósidos de esteviol de alta pureza.
EP3947409A4 (en) * 2019-03-29 2023-04-12 Conagen Inc. BIOSYNTHETIC MANUFACTURE OF UDP-RHAMNOSE
US12467039B2 (en) 2019-05-03 2025-11-11 Amyris, Inc. Kaurenoic acid 13-hydroxylase (KAH) variants and uses thereof
CN112080480B (zh) * 2019-06-14 2023-01-03 中国科学院分子植物科学卓越创新中心 糖基转移酶突变体及其应用
CN110305855B (zh) * 2019-06-26 2022-03-22 昆明理工大学 天麻GeCPR基因及其应用
KR20220123019A (ko) * 2019-12-31 2022-09-05 에어 프로틴 인코포레이티드 고단백질 식품 조성물
CN113444131B (zh) * 2021-06-24 2022-10-21 鲁东大学 N-乙酰氨基葡萄糖类化合物及其制备方法和应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013533736A (ja) * 2010-06-02 2013-08-29 エボルバ ニュートリション， インク． ステビオールグリコシドの組換え生成
WO2014086890A1 (en) * 2012-12-05 2014-06-12 Evolva Sa Steviol glycoside compositions sensory properties
WO2014122328A1 (en) * 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
WO2014122227A2 (en) * 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
JP2014524247A (ja) * 2011-08-08 2014-09-22 エヴォルヴァ エスアー． ステビオール配糖体の組換え生産

Family Cites Families (154)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149697A (ja) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
JPS59101408A (ja) 1982-12-02 1984-06-12 Junichi Iwamura 植物生長調整剤
US5198360A (en) 1990-01-19 1993-03-30 Eli Lilly And Company Dna sequence conferring a plaque inhibition phenotype
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
JPH03277275A (ja) 1990-03-28 1991-12-09 Dainippon Ink & Chem Inc 新規酵素及びその酵素を用いた配糖体の製造方法
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
US5712112A (en) 1992-11-04 1998-01-27 National Science Council Of R.O.C. Gene expression system comprising the promoter region of the alpha-amylase genes
CN1129953A (zh) 1993-07-15 1996-08-28 尼奥斯药品公司 糖组分的合成方法
US7186891B1 (en) 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
JPH10117776A (ja) 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
WO1999018224A1 (en) 1997-10-06 1999-04-15 The Centre National De Recherche Scientifique Plant fatty acid hydroxylase genes
US6255557B1 (en) 1998-03-31 2001-07-03 Her Majesty The Queen In Right Of Canada As Represented By The Ministerof Agriculture And Agri-Food Canada Stevia rebaudiana with altered steviol glycoside composition
CA2736981A1 (en) 1998-04-14 1999-10-21 Kyowa Hakko Bio Co., Ltd. Process for producing isoprenoid compounds by microorganisms
EP0955363A3 (en) 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag Dna sequences encoding enzymes involved in production of isoprenoids
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
JP4579415B2 (ja) 1998-07-06 2010-11-10 ディーシーブイ・インコーポレイテッド・ドゥーイング・ビジネス・アズ・バイオ−テクニカル・リソーシィズ ビタミン製造方法
US6531303B1 (en) 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
AR021636A1 (es) 1998-12-17 2002-07-31 Rubicon Forests Holdings Ltd Materiales y metodos para la modificacion del contenido, la composicion y el metabolismo de los isoprenoides
JP2002533090A (ja) 1998-12-23 2002-10-08 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク 植物形質転換法
JP2003527077A (ja) 1999-04-15 2003-09-16 カルジーン エルエルシー トコフェロール合成に関与するタンパク質の核酸配列
WO2000063400A2 (en) 1999-04-21 2000-10-26 The Samuel Roberts Noble Foundation Plant transformation process
ATE373715T1 (de) 1999-08-04 2007-10-15 Adelbert Bacher Isoprenoid biosynthese
WO2001012828A1 (en) 1999-08-18 2001-02-22 Paradigm Genetics, Inc. Methods and apparatus for transformation of monocotyledenous plants using agrobacterium in combination with vacuum filtration
WO2001083769A2 (en) 2000-05-03 2001-11-08 The Salk Institute For Biological Studies Crystallization of 4-diphosphocytidyl-2-c-methylerythritol synthesis
DE10027821A1 (de) 2000-06-05 2001-12-06 Adelbert Bacher Der Mevalonat-unabhängige Isoprenoidbiosyntheseweg
AU2001278098A1 (en) 2000-07-31 2002-02-13 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
EP1313867A2 (en) 2000-08-24 2003-05-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
US6689601B2 (en) 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
EP1409530A2 (en) 2000-09-19 2004-04-21 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
EP1383864B1 (en) 2000-09-29 2008-01-16 Cargill Incorporated Isoprenoid production
US6949362B2 (en) 2000-12-12 2005-09-27 E. I. Du Pont De Nemours And Company Rhodococcus cloning and expression vectors
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US20040078846A1 (en) 2002-01-25 2004-04-22 Desouza Mervyn L. Carotenoid biosynthesis
US20050003474A1 (en) 2001-01-26 2005-01-06 Desouza Mervyn L. Carotenoid biosynthesis
DE10201458A1 (de) 2001-04-11 2002-10-17 Adelbert Bacher Intermediate und Enzyme des Mevalonat-unabhängigen Isoprenoidbiosyntheseweg
US7034140B2 (en) 2001-04-24 2006-04-25 E.I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
DE60228439D1 (de) 2001-06-06 2008-10-02 Dsm Ip Assets Bv Verbesserte isoprenoid herstellung
AU2002341541A1 (en) 2001-06-22 2003-03-03 Syngenta Participations Ag Abiotic stress responsive polynucleotides and polypeptides
NZ513755A (en) 2001-08-24 2001-09-28 Ann Rachel Holmes Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs
US20040072311A1 (en) 2001-08-28 2004-04-15 Dicosimo Deana J. Production of cyclic terpenoids
US20040010815A1 (en) 2001-09-26 2004-01-15 Lange B. Markus Identification and characterization of plant genes
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
DE60329505D1 (de) 2002-08-20 2009-11-12 Suntory Holdings Ltd Neue glycosyltransferase-gene
CA2498800C (en) 2002-09-27 2012-01-03 Dsm Ip Assets B.V. Sqs gene
US7098000B2 (en) 2003-06-04 2006-08-29 E. I. Du Pont De Nemoure And Company Method for production of C30-aldehyde carotenoids
DE602004020218D1 (de) 2003-06-12 2009-05-07 Dsm Ip Assets Bv Feedback resistante mevalonate kinase
US7569389B2 (en) 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
CN101023181A (zh) 2004-05-21 2007-08-22 加利福尼亚大学董事会 提高类异戊二烯化合物的产生的方法
EP2080769A3 (en) 2004-07-02 2010-12-01 Metanomics GmbH Process for the production of fine chemicals
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
WO2006016395A1 (ja) 2004-08-09 2006-02-16 National University Corporation Tohoku University Ｕｄｐ−グルクロニル基転移酵素およびその遺伝子
US7572609B2 (en) 2004-08-19 2009-08-11 Dsm Ip Assets B.V. Production of isoprenoids
US7923552B2 (en) 2004-10-18 2011-04-12 SGF Holdings, LLC High yield method of producing pure rebaudioside A
EP1824969B1 (en) 2004-12-14 2011-03-02 DSM IP Assets B.V. Improved mevalonate kinase
WO2006092449A2 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
WO2006096392A2 (en) 2005-03-04 2006-09-14 Diversa Corporation Enzymes involved in astaxanthin, carotenoid and isoprenoid biosynthetic pathways, genes encoding them and methods of making and using them
WO2006093289A1 (ja) 2005-03-04 2006-09-08 National Institute Of Advanced Industrial Science And Technology Udp-キシロースの製造方法
US7989677B2 (en) 2005-08-17 2011-08-02 Nestec S. A. Nucleic acids and proteins associated with sucrose accumulation in coffee
CA2624230A1 (en) 2005-08-22 2007-03-01 Co2 Boost Llc A device and process to generate co2 used for indoor crop production and underwater gardening
US8293307B2 (en) 2005-10-11 2012-10-23 Purecircle Sdn Bhd Process for manufacturing a sweetener and use thereof
US8367137B2 (en) * 2005-11-23 2013-02-05 The Coca-Cola Company High-potency sweetener composition with fatty acid and compositions sweetened therewith
EP1971228A1 (en) 2005-11-23 2008-09-24 The Coca-Cola Company Natural high-potency sweetener compositions with improved temporal profile and/or flavor profile, methods for their formulation, and uses
KR100784261B1 (ko) 2006-01-02 2007-12-11 한국과학기술원 탄저균의 포자외막 단백질을 이용한 목적단백질의 미생물표면발현방법
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
CA2644273A1 (en) 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
WO2007136847A2 (en) 2006-05-19 2007-11-29 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
US7964376B2 (en) 2006-06-19 2011-06-21 Givaudan Sa Chimeric proteins and their use in methods to screen for sweet taste modulators
WO2008008256A2 (en) 2006-07-07 2008-01-17 The Regents Of The University Of California Methods for enhancing production of isoprenoid compounds by host cells
DK2066778T3 (en) 2006-09-26 2016-04-18 Amyris Biotechnologies Inc PREPARATION OF ISOPRENOIDS AND ISOPRENOID PRECURSORS
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
CN101200480B (zh) 2006-12-15 2011-03-30 成都华高药业有限公司 莱鲍迪甙a的提取方法
JP4915917B2 (ja) 2006-12-22 2012-04-11 独立行政法人農業・食品産業技術総合研究機構 ラクト−ｎ−ビオースｉ及びガラクト−ｎ−ビオースの製造方法
JP5307730B2 (ja) 2007-01-22 2013-10-02 カーギル・インコーポレイテッド 溶媒／貧溶媒晶析を用いる精製レバウディオサイドａ組成物の製法
JP2008237110A (ja) 2007-03-27 2008-10-09 Institute Of Physical & Chemical Research ステビオール合成酵素遺伝子及びステビオールの製造方法
WO2008144060A2 (en) 2007-05-17 2008-11-27 Tetravitae Bioscience, Inc. Methods and compositions for producing solvents
US20080292775A1 (en) 2007-05-22 2008-11-27 The Coca-Cola Company Delivery Systems for Natural High-Potency Sweetener Compositions, Methods for Their Formulation, and Uses
WO2009005704A1 (en) 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
GB0715226D0 (en) * 2007-08-01 2007-09-12 Cadbury Schweppes Plc Sweetener compositions
JP2009034080A (ja) 2007-08-03 2009-02-19 Sanei Gen Ffi Inc 新規糖転移酵素、及びそれを利用した配糖体の製造
US7964232B2 (en) 2007-09-17 2011-06-21 Pepsico, Inc. Steviol glycoside isomers
EP2193202A2 (en) 2007-09-21 2010-06-09 BASF Plant Science GmbH Plants with increased yield
MY155722A (en) 2007-12-03 2015-11-30 Dsm Ip Assets Bv Novel nutraceutical compositions containing stevia extract or stevia extract constituents and uses thereof
NZ586358A (en) 2007-12-27 2013-04-26 Mcneil Nutritionals Llc Synergistic sweetening compositions comprising sucralose and a purified extract of stevia
BRPI0907786B1 (pt) 2008-02-20 2021-06-01 Ceres, Inc. Método de produção de uma planta e método de aumentar o nível de tolerância a baixo teor de nitrogênio em uma planta
TWI475963B (zh) 2008-02-25 2015-03-11 可口可樂公司 甜菊糖苷ａ衍生性產物以及製造彼的方法
EP2285948B1 (en) 2008-03-03 2014-01-08 Joule Unlimited Technologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
US9005444B2 (en) 2008-05-13 2015-04-14 Cargill, Incorporated Separation of rebaudioside A from stevia glycosides using chromatography
WO2010021001A2 (en) 2008-08-19 2010-02-25 Kaushik Ramakrishnan S Process for preparing sweetener from stevia rebaudiana
CN102202523A (zh) * 2008-08-26 2011-09-28 梅里桑特公司 包含莱鲍迪甙a、赤藓糖醇、二糖碳水化合物或果糖以及口味改善量的纤维素的甜味剂组合物及其制造方法
CN107033200A (zh) 2008-10-03 2017-08-11 守田化学工业株式会社 新的甜菊醇糖苷
CN101720910B (zh) 2008-10-23 2012-07-25 大闽食品（漳州）有限公司 一种甜菊糖甙的制备方法
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
US20100297722A1 (en) 2009-05-20 2010-11-25 Board Of Trustees Of Southern Illinois University Transgenic moss producing terpenoids
DK2440661T3 (en) 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis
EP2826386B1 (en) 2009-06-16 2022-12-28 Sweet Green Fields International Co., Limited Stevia composition comprising rebaudioside A and D
CA2773134A1 (en) 2009-09-04 2011-03-10 Redpoint Bio Corporation Sweetness enhancers including rebaudioside a or d
MX2012003489A (es) 2009-09-22 2012-07-30 Redpoint Bio Corp Polimorfos novedosos de rebaudiosida c y metodos para elaborarlos y utilizarlos.
US8299224B2 (en) 2009-10-15 2012-10-30 Purecircle Sdn Bhd High-purity Rebaudioside D
ES2457641T3 (es) 2009-10-15 2014-04-28 Purecircle Sdn Bhd Procedimiento para preparar rebaudiósido D de pureza elevada
US8703224B2 (en) 2009-11-04 2014-04-22 Pepsico, Inc. Method to improve water solubility of Rebaudioside D
EP2504441B1 (en) 2009-11-23 2020-07-22 E. I. du Pont de Nemours and Company Sucrose transporter genes for increasing plant seed lipids
WO2011072246A2 (en) 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
CA2783621C (en) 2009-12-28 2018-05-29 The Coca-Cola Company Sweetness enhancers, compositions thereof, and methods for use
KR101244315B1 (ko) 2010-10-19 2013-03-14 이화여자대학교 산학협력단 에탄올―저항성 효모 유전자 및 이의 용도
WO2011140329A1 (en) 2010-05-06 2011-11-10 Ceres, Inc. Transgenic plants having increased biomass
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
EP2575499A2 (en) 2010-05-31 2013-04-10 Vib Vzw The use of transporters to modulate flavor production by yeast
US20120021111A1 (en) 2010-07-23 2012-01-26 Aexelon Therapeutics, Inc. Natural Low Caloric Sweetener Compositions for Use in Beverages, Foods and Pharmaceuticals, and Their Methods of Manufacture
ES2673730T3 (es) * 2010-10-01 2018-06-25 The Procter & Gamble Company Composiciones para el cuidado bucal con dulzor mejorado
US20120083593A1 (en) 2010-10-01 2012-04-05 Shanghai Yongyou Bioscience Inc. Separation and Purification of Stevioside and Rebaudioside A
CA2819253A1 (en) 2010-11-30 2012-06-07 Massachusetts Institute Of Technology Microbial production of natural sweeteners, diterpenoid steviol glycosides
US20130344217A1 (en) * 2010-12-29 2013-12-26 Glg Life Tech Corporation Sweetener Compositions and Methods of Making Same
MX2013010690A (es) * 2011-03-22 2014-04-30 Purecircle Usa Composicion de glicosido de esteviol glucosilado como un aumentador de gusto y sabor.
KR20130014227A (ko) 2011-07-29 2013-02-07 한국생명공학연구원 신규한 α-글루코실 스테비오사이드 및 이의 제조 방법
IN2014CN01129A (https=) 2011-08-08 2015-04-10 Evolva Sa
KR101791597B1 (ko) 2011-11-23 2017-10-30 에볼바 에스아 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
EP3009010B1 (en) 2011-12-19 2020-02-05 PureCircle SDN BHD Methods for purifying steviol glycosides
AU2013211605B2 (en) 2012-01-23 2017-04-06 Dsm Ip Assets B.V. Diterpene production
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
EP2832858B1 (en) * 2012-03-27 2020-07-08 Suntory Holdings Limited Method for producing steviol glycoside
BR112014028819A2 (pt) 2012-05-22 2017-07-25 Purecircle Sdn Bhd glicosídeos de esteviol de pureza elevada
CN103710318B (zh) 2012-09-29 2017-01-18 中国科学院上海生命科学研究院 利用微生物生产甜菊糖苷类化合物的方法
US20140342043A1 (en) * 2013-05-14 2014-11-20 Pepsico, Inc. Rebaudioside Sweetener Compositions and Food Products Sweetened with Same
US10689681B2 (en) 2013-05-31 2020-06-23 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2014191580A1 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
KR101559478B1 (ko) 2013-06-24 2015-10-13 한국생명공학연구원 효소전환법을 이용한 천연 고감미료의 제조방법
US10039834B2 (en) * 2013-07-12 2018-08-07 The Coca-Cola Company Compositions and methods using rebaudioside X to provide sweetness enhancement
EP3021689B1 (en) 2013-07-15 2021-03-24 DSM IP Assets B.V. Diterpene production
EP3024941B1 (en) 2013-07-23 2019-07-03 DSM IP Assets B.V. Diterpene production in yarrowia
CA2917615A1 (en) 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Steviol glycosides
AU2014298420B2 (en) 2013-07-31 2018-10-04 Dsm Ip Assets B.V. Recovery of steviol glycosides
US9957539B2 (en) 2013-08-02 2018-05-01 Suntory Holdings Limited Method for using hexenol glycosyl transferase
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
EP3039132A2 (en) 2013-08-30 2016-07-06 Evolva SA A method for producing modified resveratrol
WO2015051454A1 (en) 2013-10-07 2015-04-16 Vineland Research And Innovation Centre Compositions and methods for producing steviol and steviol glycosides
EP3114210A2 (en) 2014-03-07 2017-01-11 Evolva SA Methods for recombinant production of saffron compounds
US9522929B2 (en) * 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
AU2015303294A1 (en) 2014-08-11 2017-02-02 Evolva Sa. Production of steviol glycosides in recombinant hosts
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
DK3200615T3 (da) * 2014-10-03 2019-08-19 Conagen Inc Kaloriefrie sødestoffer og fremgangsmåder til syntetisering
EP3232817A4 (en) * 2014-12-17 2018-10-10 Cargill, Incorporated Steviol glycoside compounds, compositions for oral ingestion or use, and method for enhancing steviol glycoside solubility
SG11201705606PA (en) * 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
EP3718417B1 (en) * 2015-04-03 2025-09-03 DSM IP Assets B.V. Steviol glycosides
CN104845990A (zh) 2015-06-11 2015-08-19 山东大学 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201804542YA (en) * 2015-11-30 2018-06-28 Cargill Inc Steviol glycoside compositions for oral ingestion or use

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013533736A (ja) * 2010-06-02 2013-08-29 エボルバ ニュートリション， インク． ステビオールグリコシドの組換え生成
JP2014524247A (ja) * 2011-08-08 2014-09-22 エヴォルヴァ エスアー． ステビオール配糖体の組換え生産
WO2014086890A1 (en) * 2012-12-05 2014-06-12 Evolva Sa Steviol glycoside compositions sensory properties
WO2014122227A2 (en) * 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
WO2014122328A1 (en) * 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinant hosts

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019517811A (ja) * 2016-06-17 2019-06-27 カーギル インコーポレイテッド 経口摂取のためのステビオール配糖体組成物または使用
JP7107855B2 (ja) 2016-06-17 2022-07-27 カーギル インコーポレイテッド 経口摂取のためのステビオール配糖体組成物または使用
JP2022513480A (ja) * 2018-12-12 2022-02-08 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造
JP7449586B2 (ja) 2018-12-12 2024-03-14 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造

Also Published As

Publication number Publication date
US20200017896A1 (en) 2020-01-16
CN108337892B (zh) 2022-06-24
US20170369922A1 (en) 2017-12-28
EP4148137A1 (en) 2023-03-15
US11041183B2 (en) 2021-06-22
WO2016120486A1 (en) 2016-08-04
AU2024208814B2 (en) 2026-02-12
CA2973674A1 (en) 2016-08-04
SG11201705606PA (en) 2017-08-30
US10364450B2 (en) 2019-07-30
US20240026409A1 (en) 2024-01-25
AU2016211124B2 (en) 2022-04-14
AU2024208814A1 (en) 2024-09-05
AU2022205176A1 (en) 2022-08-04
CN108337892A (zh) 2018-07-27
US11807888B2 (en) 2023-11-07
US20230033436A1 (en) 2023-02-02
AU2016211124A1 (en) 2017-07-27
EP3250686A1 (en) 2017-12-06
BR112017016338A2 (en) 2018-03-27
AU2022205176B2 (en) 2024-07-11

Similar Documents

Publication Publication Date Title
CN108337892B (zh) 在重组宿主中生产甜菊醇糖苷
CN107109358B (zh) 在重组宿主中生产甜菊醇糖苷
CN105189771B (zh) 在重组宿主中有效产生甜菊醇糖苷
EP3387136B1 (en) Production of steviol glycosides in recombinant hosts
JP6379112B2 (ja) レバウディオサイドｄおよびレバウディオサイドｍの改良された産生方法
JP2019519212A (ja) 組換え宿主内でのステビオールグリコシドの産生
CN111566222A (zh) 在重组宿主中产生甜菊醇糖苷
US11396669B2 (en) Production of steviol glycosides in recombinant hosts
JP2019513392A (ja) 組み換え宿主におけるステビオールグリコシドの産生
AU2024200962B2 (en) Production of steviol glycosides in recombinant hosts

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190128

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190128

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200109

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200409

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201001

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20201215

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20210527