JP2018503388A - 組み換え宿主におけるステビオールグリコシドの生産 - Google Patents

組み換え宿主におけるステビオールグリコシドの生産 Download PDF

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Publication number
JP2018503388A
JP2018503388A JP2017540261A JP2017540261A JP2018503388A JP 2018503388 A JP2018503388 A JP 2018503388A JP 2017540261 A JP2017540261 A JP 2017540261A JP 2017540261 A JP2017540261 A JP 2017540261A JP 2018503388 A JP2018503388 A JP 2018503388A
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seq
steviol
polypeptide
cell
ent
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JP2018503388A5 (https=
Inventor
オルソン，キム
シモン，エルネスト
ミッケルセン，ミカエル ダルガート
ミッケルセン，ミカエル ダルガート
カールセン，サイモン
ドーチン，ヴェロニク
チャン リム，スウィー
チャン リム，スウィー
ダリン，ルイス
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Evolva Holding SA
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Evolva AG
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Publication of JP2018503388A publication Critical patent/JP2018503388A/ja
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Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Seasonings (AREA)
JP2017540261A 2015-01-30 2016-01-29 組み換え宿主におけるステビオールグリコシドの生産 Pending JP2018503388A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562110207P 2015-01-30 2015-01-30
US62/110,207 2015-01-30
PCT/EP2016/052007 WO2016120486A1 (en) 2015-01-30 2016-01-29 Production of steviol glycosides in recombinant hosts

Publications (2)

Publication Number Publication Date
JP2018503388A true JP2018503388A (ja) 2018-02-08
JP2018503388A5 JP2018503388A5 (https=) 2019-03-14

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Family Applications (1)

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JP2017540261A Pending JP2018503388A (ja) 2015-01-30 2016-01-29 組み換え宿主におけるステビオールグリコシドの生産

Country Status (9)

Country Link
US (4) US10364450B2 (https=)
EP (2) EP3250686A1 (https=)
JP (1) JP2018503388A (https=)
CN (1) CN108337892B (https=)
AU (3) AU2016211124B2 (https=)
BR (1) BR112017016338A2 (https=)
CA (1) CA2973674A1 (https=)
SG (1) SG11201705606PA (https=)
WO (1) WO2016120486A1 (https=)

Cited By (2)

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JP2019517811A (ja) * 2016-06-17 2019-06-27 カーギル インコーポレイテッド 経口摂取のためのステビオール配糖体組成物または使用
JP2022513480A (ja) * 2018-12-12 2022-02-08 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造

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EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
CA3128532A1 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
CA2960693A1 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN107249356B (zh) 2014-12-17 2021-08-06 嘉吉公司 用于口服摄入或使用的甜菊醇糖苷化合物、组合物以及用于增强甜菊醇糖苷溶解度的方法
EP3250686A1 (en) 2015-01-30 2017-12-06 Evolva SA Production of steviol glycosides in recombinant hosts
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
CN107666834B (zh) 2015-04-03 2021-08-24 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷
MX2018001620A (es) 2015-08-07 2018-06-11 Evolva Sa Produccion de glucosidos de esteviol en hospedadores recombinantes.
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WO2017153538A1 (en) * 2016-03-11 2017-09-14 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) * 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2017218036A1 (en) * 2016-06-17 2017-12-21 Cargill, Incorporated Steviol glycoside compositions for oral ingestion or use
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WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
KR102905512B1 (ko) 2017-02-03 2025-12-29 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
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KR20200133251A (ko) * 2018-03-16 2020-11-26 퓨어써클 유에스에이 잉크. 고-순도 스테비올 글리코사이드
CA3101651A1 (en) * 2018-06-08 2019-12-12 Purecircle Usa Inc. High-purity steviol glycosides
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CA3114953A1 (en) * 2018-10-15 2020-04-23 Amyris, Inc. Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
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BR112021019470A2 (pt) * 2019-03-29 2021-11-30 Conagen Inc Produção biossintética de udp-ramnose
MX2021013124A (es) * 2019-05-03 2022-01-24 Amyris Inc Variantes del ácido kaurenoico 13-hidroxilasa (kah) y usos del mismo.
CN112080480B (zh) * 2019-06-14 2023-01-03 中国科学院分子植物科学卓越创新中心 糖基转移酶突变体及其应用
CN110305855B (zh) * 2019-06-26 2022-03-22 昆明理工大学 天麻GeCPR基因及其应用
IL294248A (en) * 2019-12-31 2022-08-01 Air Protein Inc High protein food compositions
CN113444131B (zh) * 2021-06-24 2022-10-21 鲁东大学 N-乙酰氨基葡萄糖类化合物及其制备方法和应用

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