IN2014CN01129A - - Google Patents

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Publication number
IN2014CN01129A
IN2014CN01129A IN1129CHN2014A IN2014CN01129A IN 2014CN01129 A IN2014CN01129 A IN 2014CN01129A IN 1129CHN2014 A IN1129CHN2014 A IN 1129CHN2014A IN 2014CN01129 A IN2014CN01129 A IN 2014CN01129A
Authority
IN
India
Prior art keywords
plants
plant cells
microorganisms
crocetin
recombinant
Prior art date
Application number
Other languages
English (en)
Inventor
Shriram Raghavan
Jorgen Hansen
Shailendra Sonkar
Sathish Kumar
Kalyan K Kumar
Murali Panchapagesa
Esben Halkjaer Hansen
Klavs Riishede Hansen
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of IN2014CN01129A publication Critical patent/IN2014CN01129A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • C12N1/185Saccharomyces isolates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/46Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185Escherichia
    • C12R2001/19Escherichia coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cell Biology (AREA)
IN1129CHN2014 2011-08-08 2012-08-07 IN2014CN01129A (https=)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161521171P 2011-08-08 2011-08-08
US201161576460P 2011-12-16 2011-12-16
US201261595450P 2012-02-06 2012-02-06
PCT/IB2012/001513 WO2013021261A2 (en) 2011-08-08 2012-08-07 Methods and materials for recombinant production of saffron compounds

Publications (1)

Publication Number Publication Date
IN2014CN01129A true IN2014CN01129A (https=) 2015-04-10

Family

ID=46970359

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1129CHN2014 IN2014CN01129A (https=) 2011-08-08 2012-08-07

Country Status (12)

Country Link
US (2) US20140248668A1 (https=)
EP (1) EP2742131B1 (https=)
JP (1) JP6126597B2 (https=)
KR (2) KR101815063B1 (https=)
CN (1) CN103764818B (https=)
AU (1) AU2012293402B2 (https=)
BR (1) BR112014002924A2 (https=)
CA (1) CA2843549A1 (https=)
HK (1) HK1199286A1 (https=)
IN (1) IN2014CN01129A (https=)
RU (1) RU2676730C2 (https=)
WO (1) WO2013021261A2 (https=)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
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EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
MY199452A (en) 2013-02-06 2023-10-30 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
SG10201807693YA (en) * 2014-03-07 2018-10-30 Evolva Sa Methods for recombinant production of saffron compounds
EP3134539A1 (en) * 2014-04-25 2017-03-01 Evolva SA Methods for recombinant production of saffron compounds
CN106795477A (zh) * 2014-05-16 2017-05-31 中央研究院 用以制备虾红素的重组多核苷酸序列及其应用
CN107075522A (zh) * 2014-07-23 2017-08-18 国家新技术、能源和可持续经济发展局(Enea) 类胡萝卜素双加氧酶和在微生物和植物中以生物技术制备衍生自藏红花的化合物的方法
CA2957331A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2960693A1 (en) * 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3250686A1 (en) 2015-01-30 2017-12-06 Evolva SA Production of steviol glycosides in recombinant hosts
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
MX2018001620A (es) 2015-08-07 2018-06-11 Evolva Sa Produccion de glucosidos de esteviol en hospedadores recombinantes.
MX2018008351A (es) 2016-01-07 2018-12-06 Conagen Inc Metodos para preparar capsinoides por procesos biosinteticos.
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
CN105779406B (zh) * 2016-05-25 2019-06-18 新疆农业科学院经济作物研究所 一种藏红花酸糖基转移酶及其编码基因与应用
CN105950493B (zh) * 2016-05-25 2019-12-27 天津大学 一种工程菌及其构建方法与在制备藏红花酸中的应用
CN105950580B (zh) * 2016-05-25 2019-03-08 新疆农业科学院经济作物研究所 一种制备蛋白质UGTCs4的方法
CN109790513B (zh) * 2016-07-19 2023-04-18 科纳根公司 用于特定天然辣椒素的微生物产生的方法
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
IT201700089843A1 (it) * 2017-08-03 2019-02-03 Enea Agenzia Naz Per Le Nuove Tecnologie Lenergia E Lo Sviluppo Economico Sostenibile Geni e metodi per la produzione e compartimentazione biotecnologica di apocarotenoidi ad elevato valore aggiunto
CN110791510A (zh) * 2018-08-02 2020-02-14 中国医学科学院药用植物研究所 一种参与西红花酸合成的乙醛脱氢酶编码基因的筛选鉴定及应用
EP3878954A4 (en) * 2018-11-07 2022-10-26 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences ENZYME FOR THE BIOSYNTHESIS OF CROCIN AND USE THEREOF
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN109943493B (zh) * 2019-04-17 2021-05-18 天津大学 实现通用酶催化功能多样性的突变体菌株及其构建方法
KR102335035B1 (ko) * 2019-09-17 2021-12-06 아주대학교 산학협력단 효소반응을 이용한 크로신-4의 생산방법
EP4151732A1 (en) * 2021-09-15 2023-03-22 Universidad de Castilla-La Mancha Transgenic plants producing high levels of apocarotenoids compounds and uses thereof
CN116083288B (zh) * 2022-11-11 2025-02-18 泸州品创科技有限公司 产2,3-二氢-2,2,6-三甲基苯甲醛的肠膜明串珠菌及其应用
KR20250075325A (ko) * 2023-11-21 2025-05-28 아주대학교산학협력단 크로신 및 전구체 생산용 재조합 효모
CN118546994B (zh) * 2024-06-18 2025-01-28 华南农业大学 一种在种子胚乳生产藏红花素的转基因育种方法

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Also Published As

Publication number Publication date
AU2012293402A2 (en) 2014-03-13
WO2013021261A2 (en) 2013-02-14
RU2014109010A (ru) 2015-09-20
CN103764818B (zh) 2017-05-31
WO2013021261A3 (en) 2013-05-02
EP2742131A2 (en) 2014-06-18
BR112014002924A2 (pt) 2018-10-09
AU2012293402A1 (en) 2014-02-06
US20170306376A1 (en) 2017-10-26
JP6126597B2 (ja) 2017-05-10
JP2014524246A (ja) 2014-09-22
CN103764818A (zh) 2014-04-30
US20140248668A1 (en) 2014-09-04
AU2012293402B2 (en) 2017-08-03
KR20180004306A (ko) 2018-01-10
EP2742131B1 (en) 2018-11-28
CA2843549A1 (en) 2013-02-14
KR20140057319A (ko) 2014-05-12
KR101815063B1 (ko) 2018-01-05
KR101983115B1 (ko) 2019-05-29
RU2676730C2 (ru) 2019-01-10
HK1199286A1 (en) 2015-06-26

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