IN2014CN01129A - - Google Patents

Info

Publication number
IN2014CN01129A
IN2014CN01129A IN1129CHN2014A IN2014CN01129A IN 2014CN01129 A IN2014CN01129 A IN 2014CN01129A IN 1129CHN2014 A IN1129CHN2014 A IN 1129CHN2014A IN 2014CN01129 A IN2014CN01129 A IN 2014CN01129A
Authority
IN
India
Prior art keywords
plants
plant cells
microorganisms
crocetin
recombinant
Prior art date
Application number
Inventor
Shriram Raghavan
Jorgen Hansen
Shailendra Sonkar
Sathish Kumar
Kalyan K Kumar
Murali Panchapagesa
Esben Halkjaer Hansen
Klavs Riishede Hansen
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of IN2014CN01129A publication Critical patent/IN2014CN01129A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cell Biology (AREA)

Abstract

Recombinant microorganisms plants and plant cells are disclosed that have been engineered to express a zeaxanthin cleavage dioxygenase alone or in combination with recombinant genes encoding UDP glycosyltransferases (UGTs). Such microorganisms plants or plant cells can produce compounds from saffron such as crocetin crocetin dialdehyde crocin or picrocrocin.
IN1129CHN2014 2011-08-08 2012-08-07 IN2014CN01129A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161521171P 2011-08-08 2011-08-08
US201161576460P 2011-12-16 2011-12-16
US201261595450P 2012-02-06 2012-02-06
PCT/IB2012/001513 WO2013021261A2 (en) 2011-08-08 2012-08-07 Methods and materials for recombinant production of saffron compounds

Publications (1)

Publication Number Publication Date
IN2014CN01129A true IN2014CN01129A (en) 2015-04-10

Family

ID=46970359

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1129CHN2014 IN2014CN01129A (en) 2011-08-08 2012-08-07

Country Status (12)

Country Link
US (2) US20140248668A1 (en)
EP (1) EP2742131B1 (en)
JP (1) JP6126597B2 (en)
KR (2) KR101983115B1 (en)
CN (1) CN103764818B (en)
AU (1) AU2012293402B2 (en)
BR (1) BR112014002924A2 (en)
CA (1) CA2843549A1 (en)
HK (1) HK1199286A1 (en)
IN (1) IN2014CN01129A (en)
RU (1) RU2676730C2 (en)
WO (1) WO2013021261A2 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX369525B (en) 2013-02-06 2019-11-11 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m.
WO2014122328A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
EP3114210A2 (en) * 2014-03-07 2017-01-11 Evolva SA Methods for recombinant production of saffron compounds
EP3134539A1 (en) * 2014-04-25 2017-03-01 Evolva SA Methods for recombinant production of saffron compounds
JP2017515512A (en) * 2014-05-16 2017-06-15 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ Recombinant polynucleotide sequences for producing astaxanthin and uses thereof
CN107075522A (en) * 2014-07-23 2017-08-18 国家新技术、能源和可持续经济发展局(Enea) Carotenoid dioxygenase and the method for preparing the compound derived from safflower with biotechnology in microorganism and plant
SG11201700651RA (en) 2014-08-11 2017-02-27 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3190905A2 (en) * 2014-09-09 2017-07-19 Evolva SA Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3862426A3 (en) 2015-03-16 2021-11-17 DSM IP Assets B.V. Udp-glycosyltransferases
CN108271391A (en) 2015-08-07 2018-07-10 埃沃尔瓦公司 The generation of steviol glycoside in recombinant host
EP3377057A4 (en) 2016-01-07 2018-12-05 Conagen Inc. Methods of making capsinoids by biosynthetic processes
JP2019513392A (en) 2016-04-13 2019-05-30 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． Production of steviol glycosides in recombinant hosts
CN109312378A (en) 2016-05-16 2019-02-05 埃沃尔瓦公司 Steviol glycoside is generated in the recombination host
CN105950493B (en) * 2016-05-25 2019-12-27 天津大学 Engineering bacterium, construction method thereof and application of engineering bacterium in preparation of crocetin
CN105779406B (en) * 2016-05-25 2019-06-18 新疆农业科学院经济作物研究所 A kind of crocetin glycosyl transferase and its encoding gene and application
CN105950580B (en) * 2016-05-25 2019-03-08 新疆农业科学院经济作物研究所 A method of preparing protein UGTCs4
MX2019000860A (en) 2016-07-19 2019-06-03 Conagen Inc Method for the microbial production of specific natural capsaicinoids.
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108728477B (en) * 2017-04-24 2022-02-22 华东理工大学 Efficient transposition mutation system and construction method
IT201700089843A1 (en) * 2017-08-03 2019-02-03 Enea Agenzia Naz Per Le Nuove Tecnologie Lenergia E Lo Sviluppo Economico Sostenibile Genes and methods for the production and biotechnological compartmentation of high added value apocarotenoids
CN110791510A (en) * 2018-08-02 2020-02-14 中国医学科学院药用植物研究所 Screening identification and application of acetaldehyde dehydrogenase coding gene participating in crocetin synthesis
WO2020093285A1 (en) * 2018-11-07 2020-05-14 中国医学科学院药用植物研究所 Enzyme gene for biosynthesis of crocin and use thereof
CN109943493B (en) * 2019-04-17 2021-05-18 天津大学 Mutant strain for realizing diversity of general enzyme catalytic functions and construction method thereof
KR102335035B1 (en) * 2019-09-17 2021-12-06 아주대학교 산학협력단 Process for preparing crocin-4 using enzyme reaction
EP4151732A1 (en) * 2021-09-15 2023-03-22 Universidad de Castilla-La Mancha Transgenic plants producing high levels of apocarotenoids compounds and uses thereof

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5460949A (en) 1990-11-15 1995-10-24 Amoco Corporation Method and composition for increasing the accumulation of squalene and specific sterols in yeast
EP0747483B1 (en) * 1995-06-09 2004-03-24 DSM IP Assets B.V. Fermentative carotenoid production
JPH10117776A (en) 1996-10-22 1998-05-12 Japan Tobacco Inc Transformation of indica rice
ES2200110T3 (en) * 1996-12-02 2004-03-01 F. Hoffmann-La Roche Ag IMPROVED CAROTENIOD FERMENTATIVE PRODUCTION.
DE19916140A1 (en) * 1999-04-09 2000-10-12 Basf Ag Carotene hydroxylase and process for the preparation of xanthophyll derivatives
ATE316578T1 (en) 1999-04-15 2006-02-15 Calgene Llc NUCLEIC ACID SEQUENCES FOR PROTEINS INVOLVED IN TOCOPHEROL BIOSYNTHESIS
US20040078846A1 (en) 2002-01-25 2004-04-22 Desouza Mervyn L. Carotenoid biosynthesis
US20050003474A1 (en) 2001-01-26 2005-01-06 Desouza Mervyn L. Carotenoid biosynthesis
WO2004018694A2 (en) * 2002-08-20 2004-03-04 Sungene Gmbh & Co. Kgaa Method for producing ketocarotinoids in genetically modified organisms
ES2286504T3 (en) * 2002-09-27 2007-12-01 Dsm Ip Assets B.V. ZEAXANTINE PRODUCTION THROUGH PHAFFIA.
CA2567547C (en) 2004-05-21 2012-10-23 The Regents Of The University Of California Method for enhancing production of isoprenoid compounds
EP1956077A4 (en) * 2005-12-06 2009-02-11 Tosoh Corp Novel microorganism and method for producing carotenoid using the same
US8846374B2 (en) * 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast

Also Published As

Publication number Publication date
HK1199286A1 (en) 2015-06-26
CA2843549A1 (en) 2013-02-14
AU2012293402B2 (en) 2017-08-03
KR20180004306A (en) 2018-01-10
BR112014002924A2 (en) 2018-10-09
JP2014524246A (en) 2014-09-22
US20140248668A1 (en) 2014-09-04
KR20140057319A (en) 2014-05-12
JP6126597B2 (en) 2017-05-10
CN103764818B (en) 2017-05-31
AU2012293402A2 (en) 2014-03-13
US20170306376A1 (en) 2017-10-26
CN103764818A (en) 2014-04-30
RU2014109010A (en) 2015-09-20
EP2742131B1 (en) 2018-11-28
AU2012293402A1 (en) 2014-02-06
EP2742131A2 (en) 2014-06-18
KR101815063B1 (en) 2018-01-05
KR101983115B1 (en) 2019-05-29
WO2013021261A3 (en) 2013-05-02
WO2013021261A2 (en) 2013-02-14
RU2676730C2 (en) 2019-01-10

Similar Documents

Publication Publication Date Title
IN2014CN01129A (en)
AU2012294452A8 (en) Recombinant production of steviol glycosides
MX350771B (en) Methods and compositions for weed control.
IN2014MN00994A (en)
HUE047092T2 (en) Process for enzymatic hydrolysis of lignocellulosic material with addition of oxygen
ZA201400054B (en) Production of dha other lc-pufas in plants
EP2661664A4 (en) Natural input for spreadsheet actions
PL2917359T3 (en) Process for enzymatic hydrolysis of lignocellulosic material by using oxygen
MY167205A (en) Context reduction for context adaptive binary arithmetic coding
UA115535C2 (en) Methods and compositions for weed control
MY167872A (en) Recombinant production of steviol glycosides
MX2013008903A (en) Methods of developing terpene synthase variants.
MX2015012487A (en) Control of growth-induction-production phases.
MX337128B (en) Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases.
MX2015003700A (en) Cell for electrolyzing a liquid.
GB2487563B (en) A pellet
ZA201406125B (en) Plant hydrogen peroxide production and process using it
MX2014000507A (en) Compositons and methods for the production of isoprene.
PH12015500737A1 (en) Recombinant microorganisms for producing organic acids
EP2794848A4 (en) Reactive oxygen species-resistant microorganisms
EP3464584A4 (en) Production of monoterpene blends by unicellular photosynthetic microorganisms
LT2864493T (en) Production of enzymes for ligno-cellulosic biomass
PT2733197E (en) Biogas generating plant with tunnel fermentation chamber and installations to produce and utilise biogas
EP3240870A4 (en) Method of thermolyzing biomass in presence of hydrogen sulfide
MX2014013843A (en) Trichoderma hydrophobin production.