JP2009505679A - 殺虫性タンパク質をコードするヌクレオチド配列 - Google Patents
殺虫性タンパク質をコードするヌクレオチド配列 Download PDFInfo
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Abstract
Description
配列番号1は、好ましくは双子葉細胞における、Cry1A.105殺虫性タンパク質の発現のための合成配列である。
本実施例は、殺虫性Cry1A.105タンパク質をコードする合成ヌクレオチド配列を例示する。
この実施例は、Cry1A.105タンパク質を発現するトランスジェニックワタ植物を例示する。
この実施例は、Cry1A.105タンパク質を発現するトランスジェニックトウモロコシ植物を例示する。
Claims (36)
- 配列番号2に記載の、アミノ酸ポジション10からアミノ酸ポジション600までのアミノ酸配列を含む、殺虫性タンパク質をコードする単離されたヌクレオチド配列。
- 配列番号1および配列番号3よりなる群から選択される、請求項1に記載の単離されたヌクレオチド配列。
- 作物植物において前記殺虫性タンパク質を発現させるのに使用するための、請求項2に記載の単離されたヌクレオチド配列。
- 前記作物植物が、単子葉作物植物および双子葉作物植物よりなる群から選択される、請求項3に記載の単離されたヌクレオチド配列。
- 前記単子葉植物が、トウモロコシ、コムギ、カラスムギ、イネ、モロコシ、ミロ、ソバ、ライムギ、草(ウシノケグサ、オオアワガエリ、スズメノチャヒキ、カモガヤ、セントオーガスティン、バミューダ、ベントグラス)、およびオオムギからなる植物の群から選択される、請求項4に記載の単離されたヌクレオチド配列。
- 前記双子葉植物が、アルファルファ、リンゴ、アンズ、アスパラガス、マメ、ベリー、クロイチゴ、ブルーベリー、カノーラ、ニンジン、カリフラワー、セロリ、サクラ、ヒヨコマメ、柑橘類樹木、ワタ、ササゲ、クランベリー、キュウリ、ウリ科植物、ナス、果実樹木、ブドウ、レモン、レタス、亜麻仁、メロン、カラシナ、堅果結実樹木、オクラ、オレンジ、エンドウマメ、モモ、ピーナッツ、セイヨウナシ、セイヨウスモモ、ジャガイモ、ダイズ、カボチャ、イチゴ、サトウダイコン、ヒマワリ、サツマイモ、タバコ、トマト、カブ、および野菜からなる植物の群から選択される、請求項4に記載の単離されたヌクレオチド配列。
- 配列番号2に記載の、アミノ酸ポジション10からアミノ酸ポジション600までのアミノ酸配列を含む、単離精製された殺虫性タンパク質。
- 殺虫有効量の、請求項7に記載の単離精製されたタンパク質を含む組成物。
- 宿主細胞において、配列番号2に記載の殺虫性タンパク質を発現させるのに使用するための発現カセットであって、前記宿主細胞中で機能的なプロモーター配列および前記タンパク質をコードするヌクレオチド配列を、作動可能に連結した形で含む発現カセット。
- 前記宿主細胞が、細菌細胞、真菌細胞、哺乳動物細胞、および植物細胞よりなる群から選択される、請求項9に記載の発現カセット。
- (a)前記細菌細胞が、バチルス種細胞、エンテロバクテリアカエ種細胞、シュードモナス種細胞、クロストリジウム種細胞、およびリゾビウム種細胞、およびアグロバクテリウム種細胞よりなる群から選択され、
(b)前記植物細胞が、双子葉植物および単子葉植物からなる植物の群から選択され、前記双子葉植物が、アルファルファ、リンゴ、アンズ、アスパラガス、マメ、ベリー、クロイチゴ、ブルーベリー、カノーラ、ニンジン、カリフラワー、セロリ、サクラ、ヒヨコマメ、柑橘類樹木、ワタ、ササゲ、クランベリー、キュウリ、ウリ科植物、ナス、果実樹木、ブドウ、レモン、レタス、亜麻仁、メロン、カラシナ、堅果結実樹木、オクラ、オレンジ、エンドウマメ、モモ、ピーナッツ、セイヨウナシ、セイヨウスモモ、ジャガイモ、ダイズ、カボチャ、イチゴ、サトウダイコン、ヒマワリ、サツマイモ、タバコ、トマト、カブ、および野菜からなる群からさらに選択されており、前記単子葉植物が、トウモロコシ、コムギ、カラスムギ、イネ、モロコシ、ミロ、ソバ、ライムギ、草(ウシノケグサ、オオアワガエリ、スズメノチャヒキ、カモガヤ、セントオーガスティン、バミューダ、ベントグラス)、およびオオムギからなる群からさらに選択されている、
請求項10に記載の発現カセット。 - 前記宿主細胞が植物細胞であり、前記発現カセットが、発現エンハンサー配列、非翻訳リーダー配列、イントロン配列、葉緑体標的化ペプチドコード化配列、ならびに転写終結およびポリアデニル化配列よりなる群から選択される配列を、作動可能な連結においてさらに含む、請求項9に記載の発現カセット。
- 請求項9から請求項12のいずれかに記載の発現カセットを含むベクター。
- 配列番号2に記載の、アミノ酸ポジション10からアミノ酸ポジション600までの、殺虫活性を示すアミノ酸配列をコードするヌクレオチド配列を含む、鱗翅目昆虫の侵襲に耐性があるトランスジェニック植物または植物細胞。
- 前記トランスジェニック植物が、双子葉植物および単子葉植物よりなる群から選択され、前記双子葉植物が、アルファルファ、リンゴ、アンズ、アスパラガス、マメ、ベリー、クロイチゴ、ブルーベリー、カノーラ、ニンジン、カリフラワー、セロリ、サクラ、ヒヨコマメ、柑橘類樹木、ワタ、ササゲ、クランベリー、キュウリ、ウリ科植物、ナス、果実樹木、ブドウ、レモン、レタス、亜麻仁、メロン、カラシナ、堅果結実樹木、オクラ、オレンジ、エンドウマメ、モモ、ピーナッツ、セイヨウナシ、セイヨウスモモ、ジャガイモ、ダイズ、カボチャ、イチゴ、サトウダイコン、ヒマワリ、サツマイモ、タバコ、トマト、カブ、および野菜からなる群からさらに選択されており、前記単子葉植物が、トウモロコシ、コムギ、カラスムギ、イネ、モロコシ、ミロ、ソバ、ライムギ、草(ウシノケグサ、オオアワガエリ、スズメノチャヒキ、カモガヤ、セントオーガスティン、バミューダ、ベントグラス)、およびオオムギからなる群からさらに選択されている、請求項14に記載のトランスジェニック植物または植物細胞。
- ハマキムシ、ネキリムシ、アーミーワーム、穿孔虫、ミノムシ、およびフォーリッジフィーダーよりなる群から選択される鱗翅目昆虫の侵襲に耐性である、請求項15に記載のトランスジェニック植物または植物細胞。
- 前記鱗翅目昆虫が、フォールアーミーワーム、ヨーロピアンコーンボーラー、コーンイヤーワーム(コットンボールワームと同じ)、サウスウエスタンコーンボーラー、およびブラックカットワームからなる群からさらに選択される、請求項16に記載のトランスジェニック植物または植物細胞。
- 請求項15に記載のトランスジェニック植物または植物細胞の子孫または種子であって、前記ヌクレオチド配列を含む子孫または種子。
- 植物機能性プロモーター、および鱗翅目殺虫活性を示す、配列番号2に記載の、アミノ酸ポジション10からアミノ酸ポジション600までのアミノ酸配列をコードするヌクレオチド配列を、作動可能な連結において含む核酸配列で形質転換された1つまたは複数の植物細胞を、昆虫の餌中に提供することによる、鱗翅目昆虫の植物への侵襲を抑制する方法。
- 配列番号5および配列番号7よりなる群から選択されるポリヌクレオチド配列を含む、請求項12に記載の発現カセット。
- 生物試料において、Cry1A.105アミノ酸配列をコードするヌクレオチド配列を同定する方法であって、前記試料を、ストリンジェントなハイブリダイゼーション条件下でヌクレオチド配列とハイブリダイズするポリヌクレオチドと接触させること、および前記ポリヌクレオチドの前記ヌクレオチド配列との結合を検出することを含み、前記結合が、前記試料中の前記ヌクレオチド配列に対する診断となる方法。
- 試料においてCry1A.105タンパク質を同定する方法であって、前記試料を、前記タンパク質に特異的に結合する抗体と接触させること、および結合を検出することを含み、前記結合が、前記試料中の前記タンパク質の存在に対する診断となる方法。
- 配列番号2に記載の、約アミノ酸1から約アミノ酸612までの殺虫性タンパク質をコードする単離されたヌクレオチド配列。
- 配列番号2に記載の、約アミノ酸1から約アミノ酸610までの殺虫性タンパク質をコードする単離されたヌクレオチド配列。
- 配列番号1に記載のヌクレオチド配列に対して少なくとも約90%の同一性を示す、請求項23に記載の単離されたヌクレオチド配列。
- 配列番号3に記載のヌクレオチド配列に対して少なくとも約90%の同一性を示す、請求項23に記載の単離されたヌクレオチド配列。
- 配列番号2に記載の、約アミノ酸ポジション10から約アミノ酸ポジション600までのアミノ酸セグメントから選択される約500個から約600個の近接するアミノ酸を含むアミノ酸セグメントを含む、ハイブリッド殺虫性タンパク質。
- 殺虫有効量のCry1A.105タンパク質を含む組成物であって、植物細胞、細菌細胞、真菌細胞、コロイド、エマルジョン、種子被覆剤、ベイト、および粉末よりなる群から選択される組成物。
- 前記Cry1A.105タンパク質が、100万分の約0.5から約200部(PPM)の量で存在する、請求項28に記載の組成物。
- 前記Cry1A.105タンパク質が、約0.5から約20PPMの量で存在する、請求項29に記載の組成物。
- 植物細胞または一群の植物細胞である、請求項29に記載の組成物。
- 殺虫有効量の前記Cry1A.105タンパク質をコードするヌクレオチド配列が、配列番号1のヌクレオチド位置1401〜1420および配列番号1のヌクレオチド位置1821〜1840よりなる群から選択される配列であるか、またはこの配列に相補的であるプローブを用いて検出可能な量で存在する、請求項31に記載の組成物。
- 前記殺虫有効量の前記タンパク質が、鱗翅属植物害虫の餌中に提供される場合、前記害虫を防除するのに十分であり、前記害虫が、アンチカルシア、プソイドプルシア、ラキプルシア、ヘリオチス、ヘリコベルパ、スポドプテラ、エピノチア、およびアルミゲラよりなる群から選択される、請求項31に記載の組成物。
- 鱗翅目昆虫の侵襲から田畑の作物を保護する方法であって、鱗翅目昆虫の餌中に、殺虫有効量のCry1A.105タンパク質殺虫因子を含むトランスジェニック作物植物を成長させることによって、昆虫が前記トランスジェニック作物植物上で生存することを抑制することを含む方法。
- 前記トランスジェニック作物植物が、Cry1A.105のように、同じ昆虫種に対して有毒であり、バチルス毒素、ゼノラブダス毒素、フォトラブダス毒素、および前記昆虫種における1種または複数種の必須遺伝子の抑制に特異的なdsRNAよりなる群から選択される追加の殺虫因子をさらに含む、請求項34に記載の方法。
- 前記作物の収率が、前記1つまたは複数の殺虫因子を欠いている同質遺伝子作物の収率と比較して改善される、請求項34または35に記載の方法。
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2012
- 2012-11-27 US US13/686,320 patent/US20130095488A1/en not_active Abandoned
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2017
- 2017-07-12 AR ARP170101939A patent/AR109260A2/es unknown
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JP2009538153A (ja) * | 2006-05-26 | 2009-11-05 | モンサント テクノロジー エルエルシー | トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 |
KR102180294B1 (ko) * | 2013-06-14 | 2020-11-18 | 몬산토 테크놀로지 엘엘씨 | 탐지용 대두 유전자변형 이벤트 mon87751 및 이의 이용 방법 |
JP2016521576A (ja) * | 2013-06-14 | 2016-07-25 | モンサント テクノロジー エルエルシー | ダイズトランスジェニックイベントmon87751、その検出方法及びその使用方法 |
US10584391B2 (en) | 2013-06-14 | 2020-03-10 | Monsanto Technology Llc | Soybean transgenic event MON87751 and methods for detection and use thereof |
KR20160019534A (ko) * | 2013-06-14 | 2016-02-19 | 몬산토 테크놀로지 엘엘씨 | 탐지용 대두 유전자변형 이벤트 mon87751 및 이의 이용 방법 |
US11236399B2 (en) | 2013-06-14 | 2022-02-01 | Monsanto Technology Llc | Soybean transgenic event MON87751 and methods for detection and use thereof |
US11767568B2 (en) | 2013-06-14 | 2023-09-26 | Monsanto Technology Llc | Soybean transgenic event MON87751 and methods for detection and use thereof |
JP2017533700A (ja) * | 2014-10-16 | 2017-11-16 | モンサント テクノロジー エルエルシー | 鱗翅目害虫に有毒または阻害性の新規キメラ殺虫性タンパク質 |
US10494408B2 (en) | 2014-10-16 | 2019-12-03 | Monsanto Technology Llc | Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests |
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US11987603B2 (en) | 2014-10-16 | 2024-05-21 | Monsanto Technology Llc | Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests |
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