CN103703124B - 用于筛选α-淀粉酶的方法 - Google Patents
用于筛选α-淀粉酶的方法 Download PDFInfo
- Publication number
- CN103703124B CN103703124B CN201280032797.2A CN201280032797A CN103703124B CN 103703124 B CN103703124 B CN 103703124B CN 201280032797 A CN201280032797 A CN 201280032797A CN 103703124 B CN103703124 B CN 103703124B
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- China
- Prior art keywords
- alpha
- amylase
- seq
- parent
- preferably less
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011497570.8A CN112662734B (zh) | 2011-06-30 | 2012-06-29 | 用于筛选α-淀粉酶的方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11172286.4 | 2011-06-30 | ||
| EP11172286 | 2011-06-30 | ||
| PCT/EP2012/062785 WO2013001087A2 (en) | 2011-06-30 | 2012-06-29 | Method for screening alpha-amylases |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011497570.8A Division CN112662734B (zh) | 2011-06-30 | 2012-06-29 | 用于筛选α-淀粉酶的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103703124A CN103703124A (zh) | 2014-04-02 |
| CN103703124B true CN103703124B (zh) | 2021-01-15 |
Family
ID=46397297
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280032797.2A Active CN103703124B (zh) | 2011-06-30 | 2012-06-29 | 用于筛选α-淀粉酶的方法 |
| CN202011497570.8A Active CN112662734B (zh) | 2011-06-30 | 2012-06-29 | 用于筛选α-淀粉酶的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011497570.8A Active CN112662734B (zh) | 2011-06-30 | 2012-06-29 | 用于筛选α-淀粉酶的方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20140206026A1 (https=) |
| EP (3) | EP2726500B1 (https=) |
| JP (3) | JP6339499B2 (https=) |
| KR (1) | KR20140041801A (https=) |
| CN (2) | CN103703124B (https=) |
| AU (1) | AU2012277729B2 (https=) |
| BR (2) | BR122020009747B1 (https=) |
| DK (1) | DK3543333T3 (https=) |
| HU (1) | HUE058093T2 (https=) |
| IN (1) | IN2014CN00597A (https=) |
| MX (1) | MX351850B (https=) |
| PL (1) | PL3543333T3 (https=) |
| WO (1) | WO2013001087A2 (https=) |
Families Citing this family (305)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2540824A1 (en) | 2011-06-30 | 2013-01-02 | The Procter & Gamble Company | Cleaning compositions comprising amylase variants reference to a sequence listing |
| ES2834102T3 (es) | 2011-06-30 | 2021-06-16 | Novozymes As | Variantes de alfa-amilasa |
| WO2013171241A1 (en) | 2012-05-16 | 2013-11-21 | Novozymes A/S | Compositions comprising lipase and methods of use thereof |
| CN104379737B (zh) | 2012-06-08 | 2018-10-23 | 丹尼斯科美国公司 | 对淀粉聚合物具有增强的活性的变体α淀粉酶 |
| AU2013279440B2 (en) | 2012-06-20 | 2016-10-06 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
| US9315791B2 (en) | 2012-08-22 | 2016-04-19 | Novozymes A/S | Metalloproteases from alicyclobacillus |
| EP2888361A1 (en) | 2012-08-22 | 2015-07-01 | Novozymes A/S | Metalloprotease from exiguobacterium |
| JP6514107B2 (ja) | 2012-12-07 | 2019-05-15 | ノボザイムス アクティーゼルスカブ | 細菌の付着防止 |
| EP2934177B1 (en) | 2012-12-21 | 2017-10-25 | Novozymes A/S | Polypeptides having protease activiy and polynucleotides encoding same |
| US9902946B2 (en) * | 2013-01-03 | 2018-02-27 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| WO2014173980A2 (en) | 2013-04-23 | 2014-10-30 | Novozymes A/S | Liquid automatic dish washing detergent compositions |
| EP3461881B1 (en) | 2013-05-03 | 2024-10-09 | Novozymes A/S | Microencapsulation of detergent enzymes |
| AR096270A1 (es) | 2013-05-14 | 2015-12-16 | Novozymes As | Composiciones detergentes |
| CN105209614A (zh) * | 2013-05-17 | 2015-12-30 | 诺维信公司 | 具有α淀粉酶活性的多肽 |
| US20160083703A1 (en) | 2013-05-17 | 2016-03-24 | Novozymes A/S | Polypeptides having alpha amylase activity |
| US10538751B2 (en) | 2013-06-06 | 2020-01-21 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| WO2014202782A1 (en) * | 2013-06-21 | 2014-12-24 | Novozymes A/S | Polypeptides having amylase activity and polynucleotides encoding same |
| EP3013955A1 (en) | 2013-06-27 | 2016-05-04 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
| US10378001B2 (en) | 2013-06-27 | 2019-08-13 | Novozymes A/S | Subtilase variants and compositions comprising same |
| US20160152925A1 (en) | 2013-07-04 | 2016-06-02 | Novozymes A/S | Polypeptides Having Anti-Redeposition Effect and Polynucleotides Encoding Same |
| CN117904081A (zh) | 2013-07-29 | 2024-04-19 | 诺维信公司 | 蛋白酶变体以及对其进行编码的多核苷酸 |
| WO2015014803A1 (en) | 2013-07-29 | 2015-02-05 | Novozymes A/S | Protease variants and polynucleotides encoding same |
| US20160348084A1 (en) * | 2013-09-30 | 2016-12-01 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| WO2015049370A1 (en) | 2013-10-03 | 2015-04-09 | Novozymes A/S | Detergent composition and use of detergent composition |
| EP3083954B1 (en) | 2013-12-20 | 2018-09-26 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
| US20160312200A1 (en) * | 2013-12-20 | 2016-10-27 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| EP3114272A1 (en) | 2014-03-05 | 2017-01-11 | Novozymes A/S | Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase |
| US20160348035A1 (en) | 2014-03-05 | 2016-12-01 | Novozymes A/S | Compositions and Methods for Improving Properties of Non-Cellulosic Textile Materials with Xyloglucan Endotransglycosylase |
| US20170015950A1 (en) | 2014-04-01 | 2017-01-19 | Novozymes A/S | Polypeptides having alpha amylase activity |
| US20170204352A1 (en) * | 2014-04-01 | 2017-07-20 | Novozymes A/S | Detergent Composition |
| EP3129478B1 (en) * | 2014-04-10 | 2019-03-27 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| BR112016023188A2 (pt) | 2014-04-11 | 2018-01-16 | Novozymes As | uso de um polipeptídeo, composição detergente, métodos de lavagem para a lavagem de um item e de produção do polipeptídeo, polipeptídeo isolado, construção de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, formulação de caldo inteiro ou composição de cultura de células |
| MX2016014019A (es) | 2014-04-25 | 2017-04-06 | Basf Se | Variantes de amilasa. |
| WO2015165472A2 (en) * | 2014-04-29 | 2015-11-05 | Kobenhavns Universitet | Cold-active alpha-amylase |
| EP3760713A3 (en) | 2014-05-27 | 2021-03-31 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
| WO2015181118A1 (en) | 2014-05-27 | 2015-12-03 | Novozymes A/S | Methods for producing lipases |
| US20170121695A1 (en) * | 2014-06-12 | 2017-05-04 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| EP3155083B1 (en) | 2014-06-12 | 2021-08-11 | Novozymes A/S | Alpha-amylase variants |
| WO2016001319A1 (en) | 2014-07-03 | 2016-01-07 | Novozymes A/S | Improved stabilization of non-protease enzyme |
| EP3739029A1 (en) | 2014-07-04 | 2020-11-18 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
| CN106661566A (zh) | 2014-07-04 | 2017-05-10 | 诺维信公司 | 枯草杆菌酶变体以及编码它们的多核苷酸 |
| WO2016079110A2 (en) | 2014-11-19 | 2016-05-26 | Novozymes A/S | Use of enzyme for cleaning |
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| EP4067485A3 (en) | 2014-12-05 | 2023-01-04 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
| ES2763235T3 (es) | 2014-12-15 | 2020-05-27 | Henkel Ag & Co Kgaa | Composición detergente que comprende variantes de subtilasa |
| WO2016096996A1 (en) | 2014-12-16 | 2016-06-23 | Novozymes A/S | Polypeptides having n-acetyl glucosamine oxidase activity |
| EP3034590A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
| EP3034592A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
| EP3034597A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
| EP3034589A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
| ES2733300T3 (es) | 2014-12-17 | 2019-11-28 | Procter & Gamble | Composición detergente |
| EP3034591A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
| EP3034596B2 (en) | 2014-12-17 | 2021-11-10 | The Procter & Gamble Company | Detergent composition |
| CN118497177A (zh) | 2014-12-19 | 2024-08-16 | 诺维信公司 | 蛋白酶变体以及对其进行编码的多核苷酸 |
| EP3234093B1 (en) | 2014-12-19 | 2020-05-27 | Novozymes A/S | Protease variants and polynucleotides encoding same |
| EP3280800A1 (en) | 2015-04-10 | 2018-02-14 | Novozymes A/S | Detergent composition |
| WO2016162556A1 (en) | 2015-04-10 | 2016-10-13 | Novozymes A/S | Laundry method, use of dnase and detergent composition |
| WO2016180749A1 (en) | 2015-05-08 | 2016-11-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| CA2981409A1 (en) | 2015-05-08 | 2016-11-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| WO2016184944A1 (en) | 2015-05-19 | 2016-11-24 | Novozymes A/S | Odor reduction |
| EP3101108B1 (en) | 2015-06-04 | 2018-01-31 | The Procter and Gamble Company | Hand dishwashing liquid detergent composition |
| EP3101109B1 (en) | 2015-06-04 | 2018-03-07 | The Procter and Gamble Company | Hand dishwashing liquid detergent composition |
| CN108012543B (zh) | 2015-06-16 | 2022-01-04 | 诺维信公司 | 具有脂肪酶活性的多肽和编码它们的多核苷酸 |
| EP3310688A1 (en) | 2015-06-17 | 2018-04-25 | Novozymes A/S | Container |
| EP3106508B1 (en) | 2015-06-18 | 2019-11-20 | Henkel AG & Co. KGaA | Detergent composition comprising subtilase variants |
| CN108012544A (zh) | 2015-06-18 | 2018-05-08 | 诺维信公司 | 枯草杆菌酶变体以及编码它们的多核苷酸 |
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| CN108350443B (zh) | 2015-09-17 | 2022-06-28 | 诺维信公司 | 具有黄原胶降解活性的多肽以及编码它们的多核苷酸 |
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| CN116064474A (zh) | 2015-10-07 | 2023-05-05 | 诺维信公司 | 多肽 |
| JP2018531783A (ja) | 2015-10-14 | 2018-11-01 | ノボザイムス アクティーゼルスカブ | 水濾過膜の洗浄 |
| US10479981B2 (en) | 2015-10-14 | 2019-11-19 | Novozymes A/S | DNase variants |
| US20180171318A1 (en) | 2015-10-14 | 2018-06-21 | Novozymes A/S | Polypeptides Having Protease Activity and Polynucleotides Encoding Same |
| MX388896B (es) | 2015-10-28 | 2025-03-20 | Novozymes As | Composicion detergente que comprende variantes de amilasa y proteasa. |
| CN108473974A (zh) | 2015-11-24 | 2018-08-31 | 诺维信公司 | 具有蛋白酶活性的多肽以及编码其的多核苷酸 |
| CN108431217B (zh) | 2015-12-01 | 2022-06-21 | 诺维信公司 | 用于产生脂肪酶的方法 |
| ES2932192T3 (es) | 2015-12-07 | 2023-01-16 | Henkel Ag & Co Kgaa | Composiciones para el lavado de vajilla que comprenden polipéptidos que tienen actividad beta-glucanasa y sus usos |
| WO2017117089A1 (en) | 2015-12-28 | 2017-07-06 | Novozymes Bioag A/S | Heat priming of bacterial spores |
| JP7626576B2 (ja) * | 2015-12-30 | 2025-02-07 | ノボザイムス アクティーゼルスカブ | 酵素変異体およびこれをコードするポリヌクレオチド |
| MX2018008051A (es) | 2016-01-29 | 2018-08-23 | Novozymes As | Variantes de beta-glucanasa y polinucleotidos que las codifican. |
| BR112018069220A2 (pt) | 2016-03-23 | 2019-01-22 | Novozymes As | uso de polipeptídeo que tem atividade de dnase para tratamento de tecidos |
| CN109312270B (zh) | 2016-04-08 | 2022-01-28 | 诺维信公司 | 洗涤剂组合物及其用途 |
| CN105802940B (zh) * | 2016-04-18 | 2019-04-16 | 广西大学 | 一种地衣芽胞杆菌高温α-淀粉酶突变体及其应用 |
| EP3693449A1 (en) | 2016-04-29 | 2020-08-12 | Novozymes A/S | Detergent compositions and uses thereof |
| WO2017210188A1 (en) | 2016-05-31 | 2017-12-07 | Novozymes A/S | Stabilized liquid peroxide compositions |
| CN109715792A (zh) | 2016-06-03 | 2019-05-03 | 诺维信公司 | 枯草杆菌酶变体和对其进行编码的多核苷酸 |
| EP3257931A1 (en) | 2016-06-17 | 2017-12-20 | The Procter and Gamble Company | Detergent composition |
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| EP3284805B1 (en) | 2016-08-17 | 2020-02-19 | The Procter & Gamble Company | Cleaning composition comprising enzymes |
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| CN109996859B (zh) | 2016-09-29 | 2021-11-30 | 诺维信公司 | 含孢子的颗粒 |
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| EP3548615A1 (en) * | 2016-11-29 | 2019-10-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| KR20190086540A (ko) | 2016-12-01 | 2019-07-22 | 바스프 에스이 | 조성물 중 효소의 안정화 |
| US20190292493A1 (en) | 2016-12-12 | 2019-09-26 | Novozymes A/S | Use of polypeptides |
| CN110234747B (zh) * | 2017-02-01 | 2021-11-09 | 宝洁公司 | 包含淀粉酶变体的清洁组合物 |
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| WO2018185181A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Glycosyl hydrolases |
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| MX2014000158A (es) | 2014-02-19 |
| PL3543333T3 (pl) | 2022-06-13 |
| EP3543333A3 (en) | 2019-10-23 |
| EP3543333B1 (en) | 2022-01-05 |
| JP2020141694A (ja) | 2020-09-10 |
| MX351850B (es) | 2017-10-31 |
| EP2726500B1 (en) | 2019-03-20 |
| WO2013001087A2 (en) | 2013-01-03 |
| KR20140041801A (ko) | 2014-04-04 |
| DK3543333T3 (da) | 2022-02-14 |
| CN112662734B (zh) | 2024-09-10 |
| CN112662734A (zh) | 2021-04-16 |
| HUE058093T2 (hu) | 2022-06-28 |
| AU2012277729A1 (en) | 2014-01-16 |
| US11718840B2 (en) | 2023-08-08 |
| EP4026901A3 (en) | 2022-11-23 |
| EP4026901B1 (en) | 2025-01-22 |
| IN2014CN00597A (https=) | 2015-04-03 |
| BR122020009747B1 (pt) | 2021-07-20 |
| JP2014520517A (ja) | 2014-08-25 |
| EP3543333A2 (en) | 2019-09-25 |
| JP6339499B2 (ja) | 2018-06-06 |
| US20140206026A1 (en) | 2014-07-24 |
| WO2013001087A3 (en) | 2013-08-08 |
| EP2726500A2 (en) | 2014-05-07 |
| US20190352628A1 (en) | 2019-11-21 |
| CN103703124A (zh) | 2014-04-02 |
| EP4026901A2 (en) | 2022-07-13 |
| JP2018153191A (ja) | 2018-10-04 |
| BR112013033524A2 (pt) | 2017-02-07 |
| EP4026901C0 (en) | 2025-01-22 |
| AU2012277729B2 (en) | 2016-12-08 |
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