CN1035133A - 用与模板有关的核酸探测剂改组的测定方法 - Google Patents
用与模板有关的核酸探测剂改组的测定方法 Download PDFInfo
- Publication number
- CN1035133A CN1035133A CN88109238A CN88109238A CN1035133A CN 1035133 A CN1035133 A CN 1035133A CN 88109238 A CN88109238 A CN 88109238A CN 88109238 A CN88109238 A CN 88109238A CN 1035133 A CN1035133 A CN 1035133A
- Authority
- CN
- China
- Prior art keywords
- probe
- nucleic acid
- target
- elementary
- chain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Abstract
通过改组过量的单链探测剂的两个互补对来检测靶核酸,该探测剂与邻接靶顺序杂交。样品中的核酸受到热处理成为探测剂,将相邻的顺序连接以形成与初始靶互补的可检测融合探测剂,融合探测剂被用作进一步融合的模板,待测的被改组的物质(Spe-cies)通过将探测剂加以热处理变为靶的循环以几何级数速率增加,以与模板有关的方式连接经热处理的探测剂并将融合探测剂从模板中分离出来以形成新的模板。
Description
本发明涉及核酸杂交测定的一般领域。
核酸杂交用于检测样品中特殊核酸的存在,例如,Falkow在美国专利4358535中公开了一种杂交测定方法,在这种测定方法中单链DNA附着到一个滤器上并作标记,单链样品DNA与这个滤器连接,在样品DNA和加有标记的单链DNA之间进行杂交,被杂交的探测剂在滤器上进行测定。
Whiteley等在EP185494中公开了检测具有特征部分的靶核酸顺序的方法,在测定中用(在不太严格条件下)与特征部分互补的探测剂处理样品,然后用(在很严格的条件下)与邻接顺序互补的探测剂处理该样品,特征部分与邻接探测剂以共价键方式相连接,在未结合的探测剂被除去后检测这被结合的探测剂。
Mullis在美国专利4683202和4683195中公开了一种用于放大核酸顺序的方法,它是以一个引物处理互补核酸链,并用DNA聚合酶扩展引物以形成用于合成所希望的核酸的模板。美国专利4683195描述了检测用这个方法放大的DNA方法的特点。
我们已经发现一个用于检测样品中靶核酸顺序的存在和丰度的方法。该方法包括以几何级数速率快速循环的过量探测剂顺序的与模板有关的改组,从而使被检测的顺序的可利用性迅速增加,并最终增加了测定的灵敏度。在靶顺序处于低水平时,或在包含其他核酸顺序的样品中它是一种极其次要的组合时,这一方法的应用特别具有优越性。这个方法可以很容易地适应于自动化,这使得它对于在诊断仪器方面的应用特别有吸引力。
本发明概括地描述了用于检测样品中靶核酸顺序的方法的特征,该方法至少使用化学计量过量的至少四个单链核酸探测剂。为了方便起见,第一和第二探测剂将被称为初级探测剂,而第三和第四探测剂将被称为次级探测剂。这些探测剂有如下特征:第一探测剂能与靶核酸顺序的一个链的第一链段杂交,而第二探测剂能与靶核酸顺序的同一链的第二链段杂交。选择第一和第二探测剂,使第一探测剂的3′端与第二探测剂的5′端连接,此时这两个探测剂与靶顺序杂交,即靶顺序链的第一链段5′端的位置对应于那个链的第二链段的3′端,以便这些探测剂能连接起来。第一探测剂也能与第三探测剂杂交,而第二探测剂与第四探测剂杂交。
测定工作如下:提供DNA样品,例如单链DNA,包括两个互补靶链(一个初级靶链和一个次级靶链),如果该靶是双链的话。以四个单链形式将四个探测剂引入样品,结果这两个初级探测剂与初级靶链杂交,并且(如果这个靶是双链的话)两个次级探测剂与次级靶链杂交。此后,将两个初级探测剂连接,形成一初级合成融合探测剂顺序,并且(对于双链靶)次级探测剂被融合,形成次级合成融合探测剂顺序。DNA变性,使样品中的靶群体有效地加倍。在这一杂交循环运行时,连接和变性重复地进行,被改组的可检测融合探测剂的群体以几何级数速率增加。在靶是单链的情况下,次级探测剂直到第二个循环之前一直缺乏靶链,在这种情况下初级合成融合探测剂顺序形成这两个次级探测器的模板,而测定继续进行,如上所述。本发明使探测剂顺序的改组得以实现,按下面所述原理以几何级数速率形成所要检测的融合探测剂顺序。迅速的改组提供了极高的灵敏度,使用一个简单的议定书(Protocol)。该循环最好重复20-50次。
此外,最好是初级靶链第一部分的5′端邻近并通过磷酸酯链与初级靶链的第二部分连接,无需任何中间顺序,以提供有效的连接,特别是酶连接。对于探测剂以及对于靶来说DNA是最好的核酸。分离互补顺序的最佳办法就是通过热变性,即熔融。探测剂最好是10-200个基长。可以使用附加的(第五、第六等)探测剂,它们杂交与其他探测剂邻近的那部分;并能够以相同的方式与这些探测剂相连接。然而,四个探测剂就足够了,并且是最佳的。
上面所述的方法可以与一些灵敏的检测系统一起使用,特别是那些包括在两个不同的探测剂上的标记实体的组合物的系统。例如,探测剂上的标记实体可以是一种不溶相的专门的链合配体(即一种抗生物素蛋白官能化的不溶相的生物素),而其他探测剂上的标记实体可以是一种发色团或一种荧光团。不溶解相暴露于这个样品并洗涤后,在这个相上的发色团或荧光团的存在指示出合成融合探测剂的存在,从而指示出样品中靶的存在。
本发明还描述了完成测定的一整套材料(akit),包括探测剂、连接酶,以及分别存贮探测剂和连接酶的装置。完成该方法的装置包括容纳由靶顺序、探测剂和连接酶组成的混合物的设备,以及使混合物的温度从一个变性温度到一个允许探测剂与靶杂交的温度循环的设备。该温度最好是自动进行循环。
本发明的其他特征和优点从以下关于最佳实施例的描述和权利要求书可以很明白地显示出来。
图1是在一个杂交测定中各步骤的示意图
图2是描述所测定的被改组的探测剂的形成的曲线,是循环序数的函数。
本发明可以用图1来说明,它描述了用于检测低浓度核苷酸顺序存在的杂交测定的步骤。
该领域熟练的技术人员将认识到完成该方法中的各个步骤有许多途径。通常完成这些步骤可以用众所周知的技术,例如Maniatis等人在“分子克隆繁殖”一文〔Maniatis et al,Molecular Cloning,Cold Spring Harbor laboratory(1982)〕中所描述的那种技术。例如,双链DNA通过在80℃-105℃下1-5分钟的热变性可以变为单链。另外,还可以使用酶链分离。可以使用合成低聚核苷酸的标准技术或者通过煮解天然DNA和分离链段合成探测剂或亚链段。杂交条件将取决于所涉及链段的长度和同系度。一般说来,可以利用Wetrmar等人所描述的技术和条件〔〔Wetrmaret al.J.Mol.Biol.31:349-370(1968)〕。使用核苷酸连接酶的适当的条件和技术是众所周知的并由厂商提供。
这一系统的某些特性,虽然不是实质性的,但是比较好的、特别是只有参与与模板有关的连接的5′端应该用标准技术磷酸化,如果它们还没有磷酸化的话,以便抑制涉及5′端的连接。选择探测剂的长度和顺序,结果会出现两个探测剂不正确的连接(即出现以一种不按在予期靶上的线性顺序表示的方式的两个探测剂的连接),那些不正确地连接的探测剂将不能用作它们的互补探测剂连接的模板,因为互补探测剂的端部彼此将不能按一种合适的酶连接方式相邻。这些探测剂长度最好在10和200个基之间。最合适的连接酶是那样一些:它们至少在一套在其他方面适应该方法的反应条件下不易造成对这些探测剂的模板独立连接的催化。例如,用E.Coli DNA连接酶(从U.S.Biochemical可以买到),或用在缺乏排除了溶质的高体积密的情况下的T.Thermophilus DNA连接酶,或用在存在大约5.0mM ATP的情况下的T4DNA连接酶得到了满意的结果。见Zimmerman et al.,Proc Nat′l.Acad.Sci.80.5852(1983);Takahashi,M.,Uchida,T.J.Biochem.100,123(1986);and Ferreti et al.,Nuc.Acids Res.9,3695(1981)。
按一种用来将复式DNA分离成为它的组分链的步骤不使连接酶变性也是合适的。在通过增加温度完成变性的情况下热稳定连接酶是合适的。这样的连接酶的优越性包括减少试剂费用,减少操作复杂性,减少所加的不希望有的成分的量(这些酶通常保存在含有甘油的缓冲液中)并且这些试剂的贮存期可能比较长。
合适的热稳定连接酶是由用Takahashi等人的一般的技术〔Takahashi et al.,J.Biol.Chem.259,10041(1983)〕提纯的Thermus thermophilus(即ATCC27634)制取的连接酶。
图1表示一个检测双链靶DNA顺序的杂交测定方法,该顺序用T-T′表示。靶顺序存在于包含有许多无关DNA顺序的样品中。
本发明描述了在标准溶液中包含有用P1-P1′和P2-P2′表示的两个探测剂互补对的一套材料(akit)。这些探测剂被选择与靶顺序各部分互补。具体讲,P1与T链的A链段互补;P2与T链的B链段互补;P1′与T′链的A链段互补;P2′与T′链的B链段互补。选择这些探测剂,以便使其长度足以提供有选择的杂交,并且产生一个容易与其他样品组分区别的融合顺序。我们已经发现长度为10-200个基长的探测剂是令人满意的。以12和50个基长之间的探测剂为最好,提供大大过量的探测剂,以驱动下面所描述的反应。例如探测剂的浓度最好是每50μl的体积大约含有1012到1014个分子。
用图1A-图1D具体说明本发明方法的一个循环,首先(图1A)使样品DNA变性,然后允许杂交(图1B)。如果T存在于该样品中,存在T将碰撞到P1和P2的相当大的可能性,并且形成图1B中所示的那种物质(Species)。同样,T′将碰撞到P1′和P2′。
在这一循环的下一步是加一个连接酶,它将把相邻的探测剂端部连接起来(图1C),但一般不连接样品中DNA的平整端。连接后置样品于变性条件下(图1D),产生融合探测剂P1-P2和P1′-P2′,从这一点开始,样品准备好开始杂化-连接-变性的新的循环。
如同将从这种一个循环的例子中所看到的那样,样品从这一循环的起点的一个双链模板T1-T1′增加到两个双链模板。假定在下一个循环中具有理想效率,那么这两个合成的双链模板的每一个和原来的靶一样,将产生两个双链模板。表1给出了对于n个循环的这一级数,其中X是第一循环前T-T1对的数目。
表1
循环序号 P1-P2的数目 P1′-P2′的数目
1 1·X 1·X
2 3·X 3·X
3 7·X 7·X
4 15·X 15·X
· · ·
· · ·
· · ·
n (2n-1)X (2n-1)X
因为这种产物(Species)P1-P2(并且,如果要求的话,可以是P1′-P2′)是可检测的,所以重复的循环改善了检测的灵敏度,直到某一点。对于每一个循环来说存在一个非常小然而有限的机会,在缺乏T·或T′的时候通过平整端连接形成P1-P2或P1′-P2′。一旦出现这种现象,被连接的物质(Species)与T或T′开始时所存在的物质不能加以区别。限制循环数就减少了错误阳性读出的机会。还有,在某一点,不融合探测剂被耗尽到不能驱动所要求的反应的水平,并且融合探测剂将同不融合的探测剂杂交的机会就比较少。
图2表示描绘混合物中所存在的可检测融合探测剂的数目与循环序号的函数关系的曲线。被改组的融合探测剂的数目将按照以上公式的几何级数形式增长,这随(初始存在的靶探测剂的数目)X而定,直到增长速率显著放慢的某一水平。通过绘制相对于标准的这一关系的曲线并通过测定达到一个给定水平需要多少个循环,测定初始存在的靶的数量是可能的。
C 例1
用标准方法制备四个脱氧核糖核苷酸低聚物,这种低聚物具有如下顺序:
P1=5′GGGGATCCTCTAGAGTCGACCTGCA3′
P2=5′AATTCGAGCTCGGTACCC3′
P1′=5′GGTCGACTCTAGAGGATCCCC3′
P2′=5′GGGTACCGAGCTCG3′
P1和P2是在质粒PUC18多节区的一个链上的端接顺序,并且质粒P1′和P2′是在互补链上的端接顺序。
引子P1′和P2用多核苷酸激酶和ATP处理,以使它们的5′端磷酸化。引子P1在3′端通过用末端转移酶和α-32P-dcTP处理加放射性标记。
D 例2
制备样品,该样品含有30mM TrisCl PH8.0,100mM NaCl,1.2mM EDTA,4.0mM MgCl21.0mM二硫苏糖醇(dithiothreitol),50μg/ml牛血清清蛋白(Bovine Serum Albumin),20μg/ml Hela DNA加20μg/ml非特异性低聚核苷酸DNA(即以下20个链节:5′-ATCGATACATCAGGAATATT-3′),1μg/ml例1的各种探测剂以及不同数量的在EcoRI劈裂侧线性化的PUC18质粒DNA。这些样品的50μl等分样品按以下步骤处理:
(a)在一分钟时间内加热到100℃,以使DNA变性。
(b)在37℃保温一分钟,使DNA复性。
(c)加50个单位的E.Coli.DNA连接酶(使用厂家美国生物化学公司确定的单位)。
(d)在37℃下保温一分钟,以使适当并列的探测剂连接。
从(a)到(d)重复20到50次,移开这些等分试样加以处理以破坏残余连接酶的活性并贮存起来。用聚丙烯酰胺凝胶电泳和放射自显术分析贮存的样品。被连接的物质的可检测量的出现的次数(以循环序数计)与在这个反应开始时存在的靶分子的数目密切相关。
在下面的权利要求书中还有其他的实施例,例如RNA也可以象DNA那样使用。
在这些例子中,包含有Hela DNA和非特异低聚核苷酸以保护探测剂免遭样品中可能存在的核酸酶的水解作用。但是,对本发明来说这不是主要的。
Claims (21)
1、一种检测样品中靶核酸的方法,包括以下步骤:
(a)提供样品核酸,例如单链核酸;
(b)在该样品中提供至少四个核酸探测剂,其中i)所述第一和第二探测剂是初级探测剂,而所述第三和第四探测剂是次级核酸探测剂;ii)第一探测剂是一个能够与靶核酸的初级链的第一链段杂交的单链;iii)第二探测剂是能够与靶核酸顺序的初级链的第二链段杂交的单链;iv)当所述探测剂与所述靶核酸的所述初级链杂交时,所述靶的初级链的第一链段的5′端位于相对于所述靶的初级链的第二链段的3′端的位置,以保证第一探测剂的3′端与第二探测剂的5′端连接;v)第三探测剂能够与第一探测剂杂交;vi)第四探测剂能够与第二探测剂杂交;
(c)重复完成以下的循环:i)使所述探测剂与所述样品中的核酸杂交;ii)连接被杂交的探测剂以形成改组的融合探测剂顺序;iii)使所述样品中的DNA变性;
(d)检测改组的融合探测剂顺序,从而随着依次循环改组的融合初级和融合次探测剂的量增加。
2、根据权利要求1所述的方法,其特征在于靶顺序的所述初级链的第一链段的5′端与靶顺序的所述初级链的第二链段的3′端相邻并且通过共价键相连接,无需中间基。
3、根据权利要求1所述的方法,其特征在于探测剂通过酶相连。
4、根据权利要求3所述的方法,其特征在于探测剂通过连接酶连接。
5、根据权利要求4所述的方法,其特征在于连接酶是细菌连接酶。
6、根据权利要求5所述的方法,其特征在于连接酶是Escherichia Coli DNA连接酶或Thermus thermophilus DNA连接酶。
7、根据权利要求1所述的方法,其特征在于核酸探测剂是DNA。
8、根据权利要求1所述的方法,其特征在于靶核酸顺序是DNA。
9、根据权利要求1所述的方法,其特征在于融合核酸是通过热变性与靶顺序分离。
10、根据权利要求1所述的方法,其特征在于所述循环至少重复两次。
11、根据权利要求10所述的方法,其特征在于所述循环的重复次数在20次与50次之间。
12、根据权利要求1所述的方法,其特征在于第二探测剂5′端磷酸化,而第一探测剂的5′端没有磷酸化。
13、根据权利要求1所述的方法,其特征在于靶顺序在步骤(a)之前是双链。
14、根据权利要求1所述的方法,其特征在于所述探测剂至少用一个标记物标记。
15、根据权利要求14所述的方法,其特征在于所述的两个初级探测剂用一个标记物标记。
16、根据权利要求1或14所述的方法,其特征在于所述的两个次级探测剂用一个标记物标记。
17、根据权利要求14所述的方法,其特征在于所述的探测剂至少有一个以发色团或荧光团标记。
18、根据权利要求14所述的方法,其特征在于所述探测剂至少有一个用一个不溶相的专门键合配体标记。
19、用于完成权利要求1所述测定方法的一套器材(akit),包括所述探测剂、核酸连接酶和贮存所述探测剂、使之与所述核酸连接酶分开的装置。
20、用于完成权利要求1所述方法的装置,包括容纳由所述靶顺序、所述探测剂和连接酶组成的混合物的贮存器和使所述混合物的温度在使所述样品中的核酸变性的第一温度与使所述探测剂与所述靶杂交的第二温度之间循环的设备。
21、根据权利要求20所述的装置,其特征在于所述使温度循环的设备包括自动改变所述温度的部分。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13193687A | 1987-12-11 | 1987-12-11 | |
US131,936 | 1987-12-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN1035133A true CN1035133A (zh) | 1989-08-30 |
Family
ID=22451675
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN88109238A Pending CN1035133A (zh) | 1987-12-11 | 1988-12-10 | 用与模板有关的核酸探测剂改组的测定方法 |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0320308B1 (zh) |
JP (1) | JPH0634759B2 (zh) |
CN (1) | CN1035133A (zh) |
AT (1) | ATE96848T1 (zh) |
AU (1) | AU622426B2 (zh) |
BR (1) | BR8806539A (zh) |
CA (1) | CA1323293C (zh) |
DE (1) | DE3885422T2 (zh) |
ES (1) | ES2061694T3 (zh) |
Families Citing this family (537)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5449602A (en) * | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
US8148065B1 (en) * | 1988-04-06 | 2012-04-03 | City Of Hope | Ligation amplification of nucleic acid sequences |
CA1341584C (en) * | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
ATE144556T1 (de) * | 1988-06-24 | 1996-11-15 | Amgen Inc | Verfahren und mittel zum nachweis von nukleinsäuresequenzen |
ATE138106T1 (de) * | 1988-07-20 | 1996-06-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
US6004826A (en) * | 1988-07-20 | 1999-12-21 | David Segev | Repair-mediated process for amplifying and detecting nucleic acid sequences |
CA2345497A1 (en) | 1988-10-28 | 1990-04-28 | Genentech, Inc. | Growth hormone variants and method for forming growth hormone variants |
US6780613B1 (en) | 1988-10-28 | 2004-08-24 | Genentech, Inc. | Growth hormone variants |
CA2005927A1 (en) * | 1988-12-21 | 1990-06-21 | Chander Bahl | Method of preparing nucleotide probes using a bridging complement |
GB8902689D0 (en) * | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
US5856092A (en) * | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
US5683896A (en) | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
US5532146A (en) * | 1989-10-26 | 1996-07-02 | Hri Research, Inc. | Method for rendering ligase-based amplification products unamplifiable |
US5215899A (en) * | 1989-11-09 | 1993-06-01 | Miles Inc. | Nucleic acid amplification employing ligatable hairpin probe and transcription |
US5516663A (en) * | 1990-01-26 | 1996-05-14 | Abbott Laboratories | Ligase chain reaction with endonuclease IV correction and contamination control |
DE69118930T2 (de) * | 1990-01-26 | 1997-01-09 | Abbott Lab | Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion |
HU218095B (hu) * | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
US5650302A (en) * | 1990-05-01 | 1997-07-22 | Amgen Inc. | Method for reducing carryover contamination in an amplification procedure |
US5494810A (en) * | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
US7585512B1 (en) | 1990-05-08 | 2009-09-08 | Thomas Jefferson University | Composition and method of using tumor cells |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US6197508B1 (en) | 1990-09-12 | 2001-03-06 | Affymetrix, Inc. | Electrochemical denaturation and annealing of nucleic acid |
US5527670A (en) * | 1990-09-12 | 1996-06-18 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
US5824477A (en) * | 1990-09-12 | 1998-10-20 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
CA2052413C (en) * | 1990-09-28 | 2004-07-13 | Jeffrey L. Joseph | Nucleotides sequences useful as type-specific probes, pcr primers and lcr probes for the amplifications and detection of human papilloma virus, and related kits and methods |
DE4129653A1 (de) * | 1991-09-06 | 1993-03-11 | Boehringer Mannheim Gmbh | Verfahren zum nachweis aehnlicher nukleinsaeuren |
GB9119735D0 (en) * | 1991-09-16 | 1991-10-30 | Secr Defence | Gene probe biosensor method |
DE4132133A1 (de) * | 1991-09-26 | 1993-04-01 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen herstellung von ribonukleinsaeuren |
US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
US5348853A (en) * | 1991-12-16 | 1994-09-20 | Biotronics Corporation | Method for reducing non-specific priming in DNA amplification |
US6033854A (en) * | 1991-12-16 | 2000-03-07 | Biotronics Corporation | Quantitative PCR using blocking oligonucleotides |
JPH07503135A (ja) * | 1992-01-13 | 1995-04-06 | アボツト・ラボラトリーズ | Hiv‐1の標的核酸配列を増幅及び検出する方法 |
GB9201481D0 (en) * | 1992-01-23 | 1992-03-11 | Scient Generics Ltd | Treatment of nucleic acid material |
US6100099A (en) | 1994-09-06 | 2000-08-08 | Abbott Laboratories | Test strip having a diagonal array of capture spots |
US5869252A (en) * | 1992-03-31 | 1999-02-09 | Abbott Laboratories | Method of multiplex ligase chain reaction |
DE69331067T2 (de) * | 1992-04-06 | 2002-07-11 | Abbott Lab | Verfahren und vorrichtung zum nachweis von nukleinsäure oder analysen unter verwendung der internen totalreflexion |
US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
US5304487A (en) * | 1992-05-01 | 1994-04-19 | Trustees Of The University Of Pennsylvania | Fluid handling in mesoscale analytical devices |
US5486335A (en) * | 1992-05-01 | 1996-01-23 | Trustees Of The University Of Pennsylvania | Analysis based on flow restriction |
US5296375A (en) * | 1992-05-01 | 1994-03-22 | Trustees Of The University Of Pennsylvania | Mesoscale sperm handling devices |
US5726026A (en) * | 1992-05-01 | 1998-03-10 | Trustees Of The University Of Pennsylvania | Mesoscale sample preparation device and systems for determination and processing of analytes |
US5637469A (en) | 1992-05-01 | 1997-06-10 | Trustees Of The University Of Pennsylvania | Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems |
US6953676B1 (en) | 1992-05-01 | 2005-10-11 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
AU681082B2 (en) * | 1992-05-06 | 1997-08-21 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
US5612200A (en) * | 1992-06-24 | 1997-03-18 | Gen-Probe Incorporated | Method and kit for destroying ability of nucleic acid to be amplified |
DE4236708A1 (de) * | 1992-10-30 | 1994-05-05 | Bayer Ag | Spezifische Gensonden und Verfahren zur Diagnostik von Candida albicans |
ATE319853T1 (de) * | 1992-11-27 | 2006-03-15 | Canon Kk | Verfahren und sonde zum nachweis von nukleinsäuren |
JPH06153997A (ja) * | 1992-11-27 | 1994-06-03 | Canon Inc | 検出信号増幅による標的核酸の検出方法 |
JP3247001B2 (ja) * | 1992-12-21 | 2002-01-15 | キヤノン株式会社 | ピリリウム化合物を用いた2本鎖核酸の検出方法、ピリリウム化合物を含有するプローブ及びそれを用いた標的核酸の検出方法、新規ピリリウム化合物 |
DE4302459A1 (de) * | 1993-01-29 | 1994-08-04 | Bayer Ag | Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren |
US5985548A (en) * | 1993-02-04 | 1999-11-16 | E. I. Du Pont De Nemours And Company | Amplification of assay reporters by nucleic acid replication |
US5427928A (en) * | 1993-03-24 | 1995-06-27 | Slesarev; Alexei I. | Thermostable DNA Topoisomerase V |
US5736334A (en) * | 1993-04-12 | 1998-04-07 | Abbott Laboratories | Nucleotide sequences and process for amplifying and detection of hepatitis B viral DNA |
US6495676B1 (en) | 1993-04-13 | 2002-12-17 | Naxcor | Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents |
US6277570B1 (en) | 1993-04-13 | 2001-08-21 | Naxcor | Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents |
US5767259A (en) | 1994-12-27 | 1998-06-16 | Naxcor | Oligonucleotides containing base-free linking groups with photoactivatable side chains |
US5616464A (en) * | 1994-12-27 | 1997-04-01 | Naxcor | Nucleic acid sequence detection employing amplification probes |
US5846709A (en) * | 1993-06-15 | 1998-12-08 | Imclone Systems Incorporated | Chemical process for amplifying and detecting nucleic acid sequences |
AU7326494A (en) * | 1993-07-13 | 1995-02-13 | Abbott Laboratories | Nucleotide sequences and process for amplifying and detection of hepatitis b virus |
US5601978A (en) * | 1993-09-03 | 1997-02-11 | Abbott Laboratories | Oligonucleotides and methods for the detection of chlamydia trachomatis |
CA2126391C (en) * | 1993-09-13 | 2002-01-08 | Nobuko Yamamoto | Determination of nucleic acid by pcr, measurement of number of microbial cells, genes, or gene-copies by pcr, and measuring-kit employed for the same |
US7582421B2 (en) | 1993-11-01 | 2009-09-01 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip |
US6468742B2 (en) | 1993-11-01 | 2002-10-22 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip |
US6986985B1 (en) | 1994-01-13 | 2006-01-17 | Enzo Life Sciences, Inc. | Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct |
CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
DE69531542T2 (de) * | 1994-02-07 | 2004-06-24 | Beckman Coulter, Inc., Fullerton | Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse |
SE9400522D0 (sv) * | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
WO1995025180A1 (en) * | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
EP0684239B1 (en) * | 1994-05-26 | 2003-12-10 | Canon Kabushiki Kaisha | A method for detecting a target substance in a sample, utilizing pyrylium compound |
DE4421901A1 (de) | 1994-06-23 | 1996-01-04 | Bayer Ag | Ein DNA-Schnelltest zum Nachweis von chinolonresistenten Staphylococcus aureus Erregern in klinischem Probenmaterial |
US5622822A (en) | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
US5582988A (en) | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
EP0791075A2 (en) * | 1994-10-21 | 1997-08-27 | Abbott Laboratories | Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample |
FR2726277B1 (fr) | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
SE9403805D0 (sv) * | 1994-11-07 | 1994-11-07 | Ulf Landegren | Method of preparing oligonucleotide probes or primers, vector therefor and use thereof |
US5654141A (en) * | 1994-11-18 | 1997-08-05 | Thomas Jefferson University | Amplification based detection of bacterial infection |
US6113905A (en) * | 1995-01-06 | 2000-09-05 | The Regents Of The University Of California | Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases |
US6077510A (en) * | 1995-01-06 | 2000-06-20 | Regents Of The University Of California | Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases |
US6015660A (en) * | 1995-01-06 | 2000-01-18 | The Regents Of The University Of California | Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases |
US6312894B1 (en) | 1995-04-03 | 2001-11-06 | Epoch Pharmaceuticals, Inc. | Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders |
US5801155A (en) | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
ATE215993T1 (de) * | 1995-05-19 | 2002-04-15 | Abbott Lab | Nachweis von nukleinsäuren mit breitem dynamikbereich unter verwendung von aggregat- primer-reihen |
US5705365A (en) * | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
EP0832280A2 (en) * | 1995-06-07 | 1998-04-01 | Abbott Laboratories | Probe masking method of reducing background in an amplification reaction |
US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
JP2809601B2 (ja) * | 1995-07-13 | 1998-10-15 | 株式会社分子バイオホトニクス研究所 | 塩基配列増幅方法 |
US5989813A (en) * | 1995-07-13 | 1999-11-23 | Molecular Innovations, Inc. | Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles |
WO1997003348A1 (en) * | 1995-07-13 | 1997-01-30 | Immunological Associates Of Denver | Self-contained device integrating nucleic acid extraction, amplification and detection |
DE19525632C2 (de) * | 1995-07-14 | 1997-07-17 | Bag Biolog Analysensystem Gmbh | Sequenzspezifisches Nukleinsäurenachweisverfahren und Reagenz-System zu dessen Durchführung |
US5888731A (en) * | 1995-08-30 | 1999-03-30 | Visible Genetics Inc. | Method for identification of mutations using ligation of multiple oligonucleotide probes |
US7282327B2 (en) | 1996-01-23 | 2007-10-16 | Affymetrix, Inc. | Nucleic acid labeling compounds |
US7291463B2 (en) | 1996-01-23 | 2007-11-06 | Affymetrix, Inc. | Nucleic acid labeling compounds |
US6864059B2 (en) | 1996-01-23 | 2005-03-08 | Affymetrix, Inc. | Biotin containing C-glycoside nucleic acid labeling compounds |
AU2253397A (en) * | 1996-01-23 | 1997-08-20 | Affymetrix, Inc. | Nucleic acid analysis techniques |
EP0880598A4 (en) | 1996-01-23 | 2005-02-23 | Affymetrix Inc | RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM |
US7423143B2 (en) | 1996-01-23 | 2008-09-09 | Affymetrix. Inc. | Nucleic acid labeling compounds |
US6965020B2 (en) | 1996-01-23 | 2005-11-15 | Affymetrix, Inc. | Nucleic acid labeling compounds |
US20010018514A1 (en) | 1998-07-31 | 2001-08-30 | Mcgall Glenn H. | Nucleic acid labeling compounds |
US5994143A (en) * | 1996-02-01 | 1999-11-30 | Abbott Laboratories | Polymeric fluorophores enhanced by moieties providing a hydrophobic and conformationally restrictive microenvironment |
US5914229A (en) * | 1996-06-14 | 1999-06-22 | Sarnoff Corporation | Method for amplifying a polynucleotide |
US5939291A (en) * | 1996-06-14 | 1999-08-17 | Sarnoff Corporation | Microfluidic method for nucleic acid amplification |
US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
US6132954A (en) * | 1996-08-20 | 2000-10-17 | Baylor College Of Medicine | Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era |
US6071493A (en) | 1996-09-20 | 2000-06-06 | Baylor College Of Medicine | Method of screening for an agent that inhibits mononuclear phagocyte-plaque component complex formation |
US6043283A (en) * | 1996-09-20 | 2000-03-28 | Baylor College Of Medicine | Tyramine compounds and their neuronal effects |
DE69718268D1 (de) | 1996-10-03 | 2003-02-13 | Canon Kk | Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse |
US5919638A (en) * | 1996-10-08 | 1999-07-06 | Abbott Laboratories | Reagents and methods useful for detecting prostate tumors |
US20050202499A1 (en) | 1996-10-31 | 2005-09-15 | Billing-Medel Patricia A. | Reagents and methods useful for detecting diseases of the breast |
US6495692B1 (en) | 1996-12-10 | 2002-12-17 | Abbott Laboratories | Helium-neon excitable reticulocyte dyes derivable from halolepidines |
CA2274587A1 (en) | 1996-12-10 | 1998-06-18 | Genetrace Systems Inc. | Releasable nonvolatile mass-label molecules |
US6482795B1 (en) | 1997-01-30 | 2002-11-19 | Myriad Genetics, Inc. | Tumor suppressor designated TS10q23.3 |
US6262242B1 (en) | 1997-01-30 | 2001-07-17 | Board Of Regents, The University Of Texas System | Tumor suppressor designated TS10Q23.3 |
WO1998045420A1 (en) | 1997-04-10 | 1998-10-15 | Diagnocure Inc. | Pca3, pca3 genes, and methods of use |
US5962665A (en) | 1997-06-16 | 1999-10-05 | Abbott Laboratories | Nucleic acid primers and probes for detecting HIV-1 and HIV-2 |
US20020147143A1 (en) | 1998-03-18 | 2002-10-10 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
AU766675B2 (en) | 1998-03-30 | 2003-10-23 | Northwest Biotherapeutics, Inc. | Therapeutic and diagnostic applications based on the role of the CXCR-4 gene in tumorigenesis |
US6127121A (en) * | 1998-04-03 | 2000-10-03 | Epoch Pharmaceuticals, Inc. | Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination |
US6291174B1 (en) | 1998-06-10 | 2001-09-18 | Pig Improvement Company Uk Limited | DNA markers for pig litter size |
US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
US5998175A (en) * | 1998-07-24 | 1999-12-07 | Lumigen, Inc. | Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers |
US7029861B1 (en) | 1998-09-15 | 2006-04-18 | Board Of Regents, The University Of Texas System | LPS-response gene compositions and methods |
EP1131633A2 (en) | 1998-11-16 | 2001-09-12 | Board of Regents, The University of Texas System | Hiv-specific t-cell induction |
US6395472B1 (en) | 1999-02-05 | 2002-05-28 | Abbott Laboratories | Methods of utilizing the TT virus |
US6156515A (en) | 1999-02-09 | 2000-12-05 | Urocor, Inc. | Prostate-specific gene for diagnosis, prognosis and management of prostate cancer |
JP4772966B2 (ja) | 1999-02-12 | 2011-09-14 | ジェン−プローブ・インコーポレーテッド | 保護プローブ |
US7014994B1 (en) | 1999-03-19 | 2006-03-21 | Cornell Research Foundation,Inc. | Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process |
US6238868B1 (en) | 1999-04-12 | 2001-05-29 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology |
US6326173B1 (en) | 1999-04-12 | 2001-12-04 | Nanogen/Becton Dickinson Partnership | Electronically mediated nucleic acid amplification in NASBA |
US6309833B1 (en) | 1999-04-12 | 2001-10-30 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures |
US6531302B1 (en) | 1999-04-12 | 2003-03-11 | Nanogen/Becton Dickinson Partnership | Anchored strand displacement amplification on an electronically addressable microchip |
DE19916929A1 (de) | 1999-04-15 | 2000-10-19 | Bayer Ag | Ein automatisierbarer Schnelltest zum Nachweis von Krebserkrankungen auf der Basis von Telomerase(hTC) mRNA mit spezifischen Primern und Sonden |
US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
EP1923471B1 (en) | 1999-04-20 | 2012-12-19 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
JP2002542805A (ja) | 1999-04-30 | 2002-12-17 | ユニバーシティ オブ フロリダ | アデノ随伴ウイルス送達リボザイム組成物および使用方法 |
WO2000066783A2 (en) | 1999-05-04 | 2000-11-09 | Ortho-Clinical Diagnostics, Inc. | Rapid and efficient capture of dna from sample without using cell lysing reagent |
US8080380B2 (en) | 1999-05-21 | 2011-12-20 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
US8481268B2 (en) | 1999-05-21 | 2013-07-09 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
CA2375106C (en) | 1999-06-01 | 2014-08-19 | Huda Y. Zoghbi | Compositions and methods for the therapeutic use of an atonal-associated sequence for deafness, osteoarthritis, and abnormal cell proliferation |
ATE304061T1 (de) | 1999-07-23 | 2005-09-15 | Gen Probe Inc | Polynukleotid amplifizierungsverfahren |
AT410218B (de) | 1999-08-20 | 2003-03-25 | Baxter Ag | Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben |
NZ517121A (en) | 1999-09-13 | 2004-05-28 | Nugen Technologies Inc | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
CA2385477C (en) | 1999-09-29 | 2009-11-03 | Diagnocure Inc. | Pca3 messenger rna species in benign and malignant prostate tissues |
US7205105B2 (en) | 1999-12-08 | 2007-04-17 | Epoch Biosciences, Inc. | Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis |
US6902891B2 (en) | 1999-12-17 | 2005-06-07 | Bio Merieux | Process for labeling a nucleic acid |
US6489114B2 (en) | 1999-12-17 | 2002-12-03 | Bio Merieux | Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby |
US7250252B2 (en) | 1999-12-30 | 2007-07-31 | David Aaron Katz | Amplification based polymorphism detection |
AU2001234608A1 (en) | 2000-01-28 | 2001-08-07 | Genetrace Systems, Inc. | Methods for analysis of gene expression |
US8076063B2 (en) | 2000-02-07 | 2011-12-13 | Illumina, Inc. | Multiplexed methylation detection methods |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
EP1967595A3 (en) | 2000-02-16 | 2008-12-03 | Illumina, Inc. | Parallel genotyping of multiple patient samples |
US7306904B2 (en) | 2000-02-18 | 2007-12-11 | Olink Ab | Methods and kits for proximity probing |
SE516272C2 (sv) * | 2000-02-18 | 2001-12-10 | Ulf Landegren | Metoder och kit för analytdetektion mha proximitets-probning |
US20040002068A1 (en) | 2000-03-01 | 2004-01-01 | Corixa Corporation | Compositions and methods for the detection, diagnosis and therapy of hematological malignancies |
US6368801B1 (en) | 2000-04-12 | 2002-04-09 | Molecular Staging, Inc. | Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase |
US7262006B1 (en) | 2000-05-01 | 2007-08-28 | Ortho-Clinical Diagnostics, Inc. | Rapid and efficient capture of DNA from sample without using cell lysing reagent |
US6605451B1 (en) * | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
US7087414B2 (en) | 2000-06-06 | 2006-08-08 | Applera Corporation | Methods and devices for multiplexing amplification reactions |
US6686156B2 (en) | 2000-06-26 | 2004-02-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
AU2001273149A1 (en) | 2000-06-28 | 2002-01-08 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
CA2731495C (en) | 2000-09-01 | 2015-02-03 | Gen-Probe Incorporated | Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations |
DE60131903T2 (de) | 2000-10-24 | 2008-11-27 | The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto | Direkte multiplex charakterisierung von genomischer dna |
AU2002227982A1 (en) * | 2000-12-05 | 2002-06-18 | Syngenta Participations Ag | Method and kit for identification of nucleic acid modification enzymes and inhibitors thereof |
ATE475720T1 (de) | 2000-12-13 | 2010-08-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben |
US6794141B2 (en) | 2000-12-22 | 2004-09-21 | Arcturus Bioscience, Inc. | Nucleic acid amplification |
AU2002252279B2 (en) | 2001-03-09 | 2005-05-12 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
CA2440615A1 (en) | 2001-03-12 | 2002-09-19 | Affymetrix, Inc. | Nucleic acid labeling compounds |
WO2002072786A2 (en) | 2001-03-13 | 2002-09-19 | Corvas International, Inc. | Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon |
EP1383884A4 (en) | 2001-03-22 | 2004-12-15 | Dendreon Corp | SERINE PROTEASE CVSP14 CODING NUCLEIC ACID MOLECULES, THE CODED POLYPEPTIDES AND METHODS THEREOF |
CA2442089A1 (en) | 2001-03-27 | 2002-10-03 | Dendreon San Diego Llc | Nucleic acid molecules encoding a transmembran serine protease 9, the encoded polypeptides and methods based thereon |
EP1515982A4 (en) | 2001-05-09 | 2005-10-26 | Corixa Corp | METHODS AND COMPOSITIONS FOR THE THERAPY AND DIAGNOSIS OF PROSTATE CANCER |
US7838270B2 (en) | 2001-05-22 | 2010-11-23 | The University Of Chicago | Target-dependent transcription using deletion mutants of N4 RNA polymerase |
WO2002095002A2 (en) | 2001-05-22 | 2002-11-28 | University Of Chicago | N4 virion single-stranded dna dependent rna polymerase |
EP1481076A4 (en) | 2001-07-12 | 2005-05-11 | Illumina Inc | MULTIPLEX NUCLEIC REACTIONS |
EP1414852A2 (en) | 2001-08-10 | 2004-05-06 | Genset S.A. | Human secreted proteins, their encoding pulynucleotides, and uses thereof |
US7056671B2 (en) | 2001-08-20 | 2006-06-06 | Takara Bio Inc. | Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide |
EP1908851A3 (en) | 2001-09-19 | 2008-06-25 | Intergenetics Incorporated | Genetic analysis for stratification of cancer risk |
US20040002071A1 (en) | 2001-09-19 | 2004-01-01 | Intergenetics, Inc. | Genetic analysis for stratification of cancer risk |
US6942972B2 (en) | 2001-10-24 | 2005-09-13 | Beckman Coulter, Inc. | Efficient synthesis of protein-oligonucleotide conjugates |
US20030165935A1 (en) | 2001-11-21 | 2003-09-04 | Vann Charles S. | Digital assay |
US6589744B2 (en) | 2001-11-26 | 2003-07-08 | Syngenta Participations Ag | Method and kit for identification for nucleic acid modification enzymes and inhibitors thereof |
CA2860702C (en) | 2001-12-17 | 2019-02-26 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of inflammatory bowel disease |
EP2108707A3 (en) | 2002-05-17 | 2010-04-14 | The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services | Molecular identification of Aspergillus Species |
DK1636561T3 (da) | 2002-07-15 | 2011-05-16 | Univ Texas | Kombinatorisk proteinbiblioteksscreening ved hjælp af periplasmatisk ekspression |
US7601493B2 (en) | 2002-07-26 | 2009-10-13 | Nanogen, Inc. | Methods and apparatus for screening and detecting multiple genetic mutations |
EP1578396A4 (en) | 2002-08-12 | 2007-01-17 | David Kirn | METHODS AND COMPOSITIONS RELATING TO POXVIRUS AND CANCER |
AU2002951411A0 (en) | 2002-09-16 | 2002-09-26 | The University Of Sydney | Genotype screen |
BR0314629A (pt) | 2002-09-19 | 2005-08-02 | Us Gov Health & Human Serv | Polipeptìdeos de p. ariasi polipeptìdeos de p. perniciosus e métodos e uso |
EP2233494A3 (en) | 2002-09-20 | 2013-05-29 | Yale University | Riboswitches, methods for their use, and compositions for use with riboswitches |
JP4694201B2 (ja) | 2002-09-20 | 2011-06-08 | インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド | アントラキノン消光色素、それらの製造方法及び使用 |
BR0315872A (pt) | 2002-10-29 | 2006-07-18 | Us Gov Health & Human Serv | Polipeptìdeos de lutzomyia longipalpis e processos de utilização e método de uso |
US7807802B2 (en) | 2002-11-12 | 2010-10-05 | Abbott Lab | Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae |
JP2006508662A (ja) | 2002-12-04 | 2006-03-16 | アプレラ コーポレイション | ポリヌクレオチドの多重増幅 |
US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
SI1589934T1 (sl) | 2003-01-06 | 2016-02-29 | Corixa Corporation Corporation Service Company | Določene aminoalkil glukozaminid fosfat spojine in njih uporaba |
US7960522B2 (en) | 2003-01-06 | 2011-06-14 | Corixa Corporation | Certain aminoalkyl glucosaminide phosphate compounds and their use |
WO2004070056A2 (en) | 2003-02-07 | 2004-08-19 | Diagnocure Inc. | Method to detect prostate cancer in a sample |
JP2006522022A (ja) | 2003-02-14 | 2006-09-28 | ザ キュレイターズ オブ ザ ユニバーシティー オブ ミズーリ | プロテアソーム干渉に関連する避妊法および組成物 |
WO2004081225A2 (en) | 2003-03-07 | 2004-09-23 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process |
US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
EP1613778B1 (en) | 2003-04-14 | 2014-11-26 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US7670810B2 (en) | 2003-06-20 | 2010-03-02 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
US20040259100A1 (en) | 2003-06-20 | 2004-12-23 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
CN1882688B (zh) | 2003-07-25 | 2014-07-16 | 安比恩股份有限公司 | 用于分离小rna分子的方法和组合物 |
US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
US7871768B2 (en) | 2003-08-04 | 2011-01-18 | The Hospital For Sick Children | Lafora's disease gene |
US7348146B2 (en) | 2003-10-02 | 2008-03-25 | Epoch Biosciences, Inc. | Single nucleotide polymorphism analysis of highly polymorphic target sequences |
PT1687609E (pt) | 2003-10-28 | 2015-03-02 | Epoch Biosciences Inc | Sondas fluorescentes para a detecção de adn por hibridação com uma maior sensibilidade e menor ruído de fundo |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US7439341B2 (en) | 2003-11-14 | 2008-10-21 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
ES2357956T3 (es) | 2003-12-06 | 2011-05-04 | Abbott Laboratories | Método y sistema para analizar reacciones usando un sistema de información. |
TW201037080A (en) | 2003-12-25 | 2010-10-16 | Danform Kk | Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same |
EP1713925B9 (en) | 2004-01-07 | 2012-09-05 | Hitachi Chemical Research Center, Inc. | Primers and probes for the detection of hiv |
DE602005020421D1 (de) | 2004-02-19 | 2010-05-20 | Helicos Biosciences Corp | Verfahren zur analyse von polynukleotidsequenzen |
CA2566962A1 (en) | 2004-05-21 | 2005-12-01 | Atonomics A/S | Surface acoustic wave sensor comprising a hydrogel |
CN102453763A (zh) | 2004-06-03 | 2012-05-16 | 阿什洛米克斯控股有限公司 | 诊断应激的药物和方法 |
EP1630236A3 (de) | 2004-08-30 | 2006-05-03 | FBF- Förderverein Biologieforschung der Deutschen Schweineproduktion e.V. | Hernia inguinalis/scrotalis assozieerte Genomregionen |
PL1794327T3 (pl) | 2004-09-14 | 2017-04-28 | Argos Therapeutics, Inc. | Niezależna od szczepu amplifikacja patogenów i szczepionki przeciwko nim |
ES2345993T3 (es) | 2004-09-14 | 2010-10-07 | The Regents Of The University Of Colorado, A Body Corporate | Metodo para el tratamiento con bucindolol basado en direccionamiento genetico. |
CA2587670A1 (en) | 2004-11-01 | 2006-05-11 | George Mason University | Compositions and methods for diagnosing colon disorders |
CA2491067A1 (en) | 2004-12-24 | 2006-06-24 | Stichting Katholieke Universiteit | Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer |
US8309303B2 (en) | 2005-04-01 | 2012-11-13 | Qiagen Gmbh | Reverse transcription and amplification of RNA with simultaneous degradation of DNA |
US8124335B2 (en) | 2005-05-06 | 2012-02-28 | Gen-Probe Incorporated | Compositions and assays to detect influenza virus A and B nucleic acids |
AU2006246975B2 (en) | 2005-05-17 | 2011-08-11 | Ozgene Pty Ltd | Sequential cloning system |
US7476735B2 (en) | 2005-05-20 | 2009-01-13 | Integrated Dna Technologies, Inc. | Compounds and methods for labeling oligonucleotides |
WO2007006091A1 (en) | 2005-07-07 | 2007-01-18 | Athlomics Pty Ltd | Polynucleotide marker genes and their expression, for diagnosis of endotoxemia |
US7494788B2 (en) | 2005-07-11 | 2009-02-24 | Molecular Kinetics, Inc. | Entropic bristle domain sequences and their use in recombinant protein production |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8980246B2 (en) | 2005-09-07 | 2015-03-17 | Sillajen Biotherapeutics, Inc. | Oncolytic vaccinia virus cancer therapy |
EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
US7608395B2 (en) | 2005-09-15 | 2009-10-27 | Baylor Research Institute | Systemic lupus erythematosus diagnostic assay |
EP2351843B1 (en) | 2005-10-14 | 2013-06-12 | The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services | Rabies virus vector systems and compositions and methods thereof |
EP2319941A3 (en) | 2005-10-21 | 2011-08-17 | GeneNews Inc. | Method and apparatus for correlating levels of biomarker products with disease |
JP2009516525A (ja) | 2005-11-22 | 2009-04-23 | プラント リサーチ インターナショナル ベー. フェー. | 複合的核酸検出法 |
EP1798292A1 (en) | 2005-12-19 | 2007-06-20 | Nutreco Nederland B.V. | Methods for improving turkey meat production |
US7981606B2 (en) | 2005-12-21 | 2011-07-19 | Roche Molecular Systems, Inc. | Control for nucleic acid testing |
EP1974052A2 (en) | 2005-12-21 | 2008-10-01 | Yale University | Methods and compositions related to the modulation of riboswitches |
CA3185510A1 (en) | 2006-02-13 | 2007-08-23 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primers and probes for detection and discrimination of types and subtypes of influenza viruses |
BRPI0708865A8 (pt) | 2006-03-14 | 2019-01-22 | Univ Oregon Health & Science | métodos para produzir uma resposta imune à tuberculose |
EP2007905B1 (en) | 2006-03-15 | 2012-08-22 | Micronics, Inc. | Integrated nucleic acid assays |
CA2651815A1 (en) | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
GB0611116D0 (en) | 2006-06-06 | 2006-07-19 | Oxford Genome Sciences Uk Ltd | Proteins |
JP5635772B2 (ja) | 2006-08-01 | 2014-12-03 | ジェン−プロウブ インコーポレイテッド | 核酸の非特異的標的の捕捉方法 |
US8080645B2 (en) | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
US8097419B2 (en) | 2006-09-12 | 2012-01-17 | Longhorn Vaccines & Diagnostics Llc | Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009 |
US20080124728A1 (en) | 2006-09-12 | 2008-05-29 | Tony Baker | Removal of Molecular Assay Interferences for Nucleic Acids Employing Buffered Solutions of Chaotropes |
US9481912B2 (en) | 2006-09-12 | 2016-11-01 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and identifying nucleic acid sequences in biological samples |
EP2064229B1 (en) | 2006-09-15 | 2015-08-12 | Ottawa Hospital Research Institute | Oncolytic rhabdovirus |
CA2671264C (en) | 2006-11-30 | 2015-11-24 | Research Development Foundation | Improved immunoglobulin libraries |
JP2010514692A (ja) | 2006-12-20 | 2010-05-06 | バイエル ヘルスケア リミティド ライアビリティ カンパニー | 癌の治療に有用なヒドロキシメチルフェニルピラゾリル尿素化合物 |
WO2008080029A2 (en) | 2006-12-21 | 2008-07-03 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
US20120144508A1 (en) | 2006-12-21 | 2012-06-07 | Ben Hayes | Artificial selection method and reagents |
EP1939303A1 (en) | 2006-12-27 | 2008-07-02 | Wageningen Universiteit | Method for detecting cyst nematodes |
MX351975B (es) | 2006-12-27 | 2017-11-06 | Emory Univ Star | Composiciones y metodos para el tratamiento de infecciones y tumores. |
NL1033431C2 (nl) | 2007-02-20 | 2008-08-21 | Expressive Res Bv | Bepaling van kwaliteitskenmerken bij land- of tuinbouwproducten. |
EP3118221B1 (en) | 2007-02-26 | 2019-08-21 | Oxford BioTherapeutics Ltd | Proteins |
WO2008104803A2 (en) | 2007-02-26 | 2008-09-04 | Oxford Genome Sciences (Uk) Limited | Proteins |
EP2125868B1 (en) | 2007-02-28 | 2015-06-10 | The Govt. Of U.S.A. As Represented By The Secretary Of The Department Of Health And Human Services | Brachyury polypeptides and methods for use |
PL2191001T3 (pl) | 2007-04-09 | 2017-01-31 | University Of Florida Research Foundation, Inc. | Kompozycje wektora RAAV mające białka kapsydu zmodyfikowane tyrozyną i sposoby ich zastosowania |
US8629245B2 (en) | 2007-05-01 | 2014-01-14 | Research Development Foundation | Immunoglobulin Fc libraries |
US20110209231A1 (en) | 2007-05-16 | 2011-08-25 | Timothy James Doran | Treatment and prevention of influenza |
US8008010B1 (en) | 2007-06-27 | 2011-08-30 | Applied Biosystems, Llc | Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor |
US11041215B2 (en) | 2007-08-24 | 2021-06-22 | Longhorn Vaccines And Diagnostics, Llc | PCR ready compositions and methods for detecting and identifying nucleic acid sequences |
EP2240606B1 (en) | 2008-01-14 | 2016-10-12 | Applied Biosystems, LLC | Compositions, methods, and kits for detecting ribonucleic acid |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US9695469B2 (en) | 2008-03-17 | 2017-07-04 | Stichting Genetwister Ip | Expression-linked gene discovery |
WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
EP2281059B1 (en) | 2008-04-10 | 2015-01-14 | Genenews Corporation | Method and apparatus for determining a probability of colorectal cancer in a subject |
EP2151502A1 (en) | 2008-07-30 | 2010-02-10 | Lohmann Tierzucht GmbH | Genetic variations associated with feather pecking behaviour in avians |
US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
CA2732750A1 (en) | 2008-09-22 | 2010-03-25 | The Government Of The United States Of America D.B.A.The Department Of V Eterans Affairs | Methods for detecting a mycobacterium tuberculosis infection |
CA2744449C (en) | 2008-11-28 | 2019-01-29 | Emory University | Methods for the treatment of infections and tumors |
WO2010068102A1 (en) | 2008-12-10 | 2010-06-17 | Monsanto Invest N.V. | Novel plant virus |
MX337685B (es) | 2008-12-19 | 2016-03-15 | Enza Zaden Beheer Bv | Metodo para reproducir plantas de pepino resistentes al virus del amarilleo y enanismo de cucurbitaceas (cysdv). |
WO2010084408A2 (en) | 2009-01-21 | 2010-07-29 | Oxford Biotherapeutics Ltd. | Pta089 protein |
EP2384368B1 (en) | 2009-01-30 | 2016-01-27 | Kantonsspital Aarau AG | Gene dosage analysis |
AU2010215061B2 (en) | 2009-02-17 | 2016-07-14 | E.D.G. Innovations and Consulting Pty Ltd | Assay for determining epigenetic profiles of markers of Fragile X alleles |
WO2010096713A2 (en) | 2009-02-20 | 2010-08-26 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Method for the diagnosis of age-associated vascular disorders |
US8921039B2 (en) | 2009-02-26 | 2014-12-30 | Gen-Probe Incorporated | Assay for detection of human parvovirus nucleic acid |
CA2757300C (en) | 2009-04-01 | 2018-01-09 | Dxterity Diagnostics Incorporated | Chemical ligation dependent probe amplification (clpa) |
EP2421552B8 (en) | 2009-04-22 | 2017-01-25 | Indiana University Research and Technology Corporation | Collagen v for use in the treatment of asthma |
WO2010138908A1 (en) | 2009-05-29 | 2010-12-02 | Ventana Medical Systems, Inc. | Igfir gene copy number as a prognostic marker in a non-small cell lung cancer |
EP2446053A1 (en) | 2009-05-29 | 2012-05-02 | Ventana Medical Systems, Inc. | Methods of scoring gene copy number in a biological sample using in situ hybridization |
WO2010138926A1 (en) | 2009-05-29 | 2010-12-02 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Nucleic acids for detection and discrimination of genotypes of chlamydophila psittaci |
WO2011008530A2 (en) | 2009-06-29 | 2011-01-20 | Luminex Corporation | Chimeric primers with hairpin conformations and methods of using same |
EP2449132B1 (en) | 2009-07-01 | 2015-05-13 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
EP2272980A1 (en) | 2009-07-09 | 2011-01-12 | Bioesplora SRL | Molecular probes for target nucleic acid detection and quantification |
SG177687A1 (en) | 2009-07-17 | 2012-02-28 | Bioatla Llc | Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts |
US8846349B2 (en) | 2009-07-22 | 2014-09-30 | E.I. Du Pont De Nemours And Company | Sequences and their use for detection and characterization of E. coli O157:H7 |
US9409983B2 (en) | 2009-07-23 | 2016-08-09 | The Board Of Trustess Of The University Of Illinois | Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases |
EP2294912A1 (en) | 2009-09-04 | 2011-03-16 | Wageningen Universiteit | Method for increasing the level of zeaxanthin in a plant line, method for selecting a plant or part thereof, including a seed and tuber, and use thereof |
US9512481B2 (en) | 2009-09-11 | 2016-12-06 | The Regents Of The University Of Colorado, A Body Corporate | Polymorphisms in the PDE3A gene |
ES2848650T3 (es) | 2009-09-14 | 2021-08-11 | Sillajen Biotherapeutics Inc | Terapia combinada contra el cáncer con virus vaccinia oncolítico |
WO2011038290A2 (en) | 2009-09-25 | 2011-03-31 | The U. S. A., As Represented By The Secretary, Department Of Health And Human Services | Neutralizing antibodies to hiv-1 and their use |
EP3236264A3 (en) | 2009-10-13 | 2017-11-08 | Nanostring Technologies, Inc | Protein detection via nanoreporters |
US9181306B2 (en) | 2009-10-16 | 2015-11-10 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Insertion of foreign genes in rubella virus and their stable expression in a live, attenuated viral vaccine |
GB2474520B (en) | 2009-10-19 | 2015-08-26 | London & General Packaging Ltd | Spray dispenser |
WO2011049439A1 (en) | 2009-10-19 | 2011-04-28 | Universiteit Twente | Method for selecting bone forming mesenchymal stem cells |
EP2682482A1 (en) | 2009-10-26 | 2014-01-08 | Abbott Laboratories | Diagnostic methods for determining prognosis of non-small cell lung cancer |
ES2644277T3 (es) | 2009-10-26 | 2017-11-28 | Abbott Molecular Inc. | Métodos de diagnóstico para determinar la prognosis del cáncer de pulmón de células no pequeñas |
EP2496605A1 (en) | 2009-11-02 | 2012-09-12 | Oxford Biotherapeutics Ltd. | Ror1 as therapeutic and diagnostic target |
EP2502076B1 (en) | 2009-11-20 | 2018-04-18 | Oregon Health and Science University | Methods for detecting a mycobacterium tuberculosis infection |
MX337062B (es) | 2009-12-10 | 2016-02-11 | Ottawa Hospital Res Inst | Rabdovirus oncolítico. |
US20130131161A1 (en) | 2009-12-23 | 2013-05-23 | Michael R. Bristow | Methods and compositions for cardiovascular diseases and conditions |
CN102725424B (zh) | 2010-01-25 | 2014-07-09 | Rd生物科技公司 | 靶核酸的自折叠扩增 |
CN102740976B (zh) | 2010-01-29 | 2016-04-20 | 精密公司 | 取样-应答微流体盒 |
US8916345B2 (en) | 2010-03-26 | 2014-12-23 | Integrated Dna Technologies, Inc. | Methods for enhancing nucleic acid hybridization |
US9506057B2 (en) | 2010-03-26 | 2016-11-29 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
WO2011133433A2 (en) | 2010-04-16 | 2011-10-27 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Real time pcr assay for detection of bacterial respiratory pathogens |
WO2011130728A1 (en) | 2010-04-17 | 2011-10-20 | Bayer Healthcare Llc | Synthetic metabolites of fluoro substituted omega-carboxyaryl diphenyl urea for the treatment and prevention diseases and conditions |
US9828637B2 (en) | 2010-04-18 | 2017-11-28 | Wake Forest University Health Sciences | Methods of predicting predisposition to or risk of kidney disease |
SI2561067T1 (sl) | 2010-04-23 | 2019-04-30 | University Of Florida Research Foundation, Inc. | Sestavki RAAV-gvanilat ciklaze in postopki za zdravljenje Leberjeve kongenitalne amavroze-1 (LCA1) |
US8828688B2 (en) | 2010-05-27 | 2014-09-09 | Affymetrix, Inc. | Multiplex amplification methods |
WO2011159835A1 (en) | 2010-06-16 | 2011-12-22 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Antibodies to endoplasmin and their use |
WO2011162612A1 (en) | 2010-06-25 | 2011-12-29 | Wageningen Universiteit | Method for modulating the level of phosphorylation of starch in a plant line, method for selecting a plant or part thereof, including a seed and tuber, and use thereof |
US20130316338A1 (en) | 2010-06-29 | 2013-11-28 | The United States Government As Represented By The Department Of Veterans Affairs | CCR6 As A Biomarker of Alzheimer's Disease |
US9234249B2 (en) | 2010-07-12 | 2016-01-12 | Gen-Probe Incorporated | Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids |
AU2011277178B2 (en) | 2010-07-16 | 2016-10-20 | Stichting Vumc | A method of analysing a blood sample of a subject for the presence of a disease marker |
EP2418286A1 (en) | 2010-08-10 | 2012-02-15 | QIAGEN GmbH | Improved method for isothermal amplification of nucleic acids |
WO2012019235A1 (en) | 2010-08-11 | 2012-02-16 | Murdoch Childrens Research Institute | Treatment and diagnosis of epigenetic disorders and conditions |
PL2611925T3 (pl) | 2010-08-30 | 2018-05-30 | Dow Agrosciences, Llc | Wzmacniacz z bacilokształtnego wirusa trzciny cukrowej (SCBV) i jego zastosowanie w genomice funkcjonalnej roślin |
BR112013004691A2 (pt) | 2010-08-30 | 2017-11-21 | Dow Agrosciences Llc | construto de dna de ativação gênica marcada de milho, população marcada de plantas de milho, método para geração da mesma e método para gerar uma planta de milho marcada |
EP2614149B1 (en) | 2010-09-07 | 2015-04-08 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
ES2707341T3 (es) | 2010-09-13 | 2019-04-03 | Clinical Genomics Pty Ltd | Diagnóstico de cáncer mediante marcador de metilación |
WO2012049279A1 (en) | 2010-10-14 | 2012-04-19 | Universitaet Des Saarlandes | MEANS AND METHODS APPLYING SINGLE NUCLEOTIDE PRIMER EXTENSION WITH ION PAIR-, REVERSED-PHASE HPLC (SIRPH) FOR THE DIAGNOSIS OF SNPs |
US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
US9714940B2 (en) | 2010-10-22 | 2017-07-25 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
WO2013082164A1 (en) | 2011-11-28 | 2013-06-06 | Life Technologies Corporation | Enhanced ligation reactions |
BR112013017096A2 (pt) | 2011-01-04 | 2020-09-01 | Jennerex Inc. | composição e métodos de indução de resposta citotóxica dependente de complemento mediado por anticorpo específico de tumor em animal tendo tumor, de geração de anticorpos in vivo, de inibição do crescimento ou morte de célula de câncer, para tratar indivíduo com câncer, para adaptar de terapia de câncer para indivíduo com câncer e para identificar antígeno específico de tumor |
AU2012209074A1 (en) | 2011-01-25 | 2013-07-11 | Almac Diagnostics Limited | Colon cancer gene expression signatures and methods of use |
WO2012106385A2 (en) | 2011-01-31 | 2012-08-09 | Apprise Bio, Inc. | Methods of identifying multiple epitopes in cells |
CN103443338B (zh) | 2011-02-02 | 2017-09-22 | 华盛顿大学商业化中心 | 大规模平行邻接作图 |
US8952132B2 (en) | 2011-02-07 | 2015-02-10 | Research Development Foundation | Engineered immunoglobulin FC polypeptides |
MX2013009863A (es) | 2011-02-28 | 2013-12-06 | Univ Iowa Res Found | Cambios de la hormona anti-mulleriana en el embarazo y prediccion de sexo y de resultados adversos en el embarazo. |
US9057107B2 (en) | 2011-03-08 | 2015-06-16 | King Abdullah University Of Science And Technology | Molecular biomarker set for early detection of ovarian cancer |
USRE49975E1 (en) | 2011-03-10 | 2024-05-21 | Gen-Probe Incorporated | Methods and compositions for the selection and optimization of oligonucleotide tag sequences |
NL2006378C2 (en) | 2011-03-11 | 2012-09-12 | Univ Wageningen | Tyclv resistance. |
CA2830461C (en) | 2011-03-18 | 2021-06-29 | Thomas Wurdinger | A method of analysing a blood sample of a subject for the presence of a disease marker |
US9150644B2 (en) | 2011-04-12 | 2015-10-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Human monoclonal antibodies that bind insulin-like growth factor (IGF) I and II |
US20140106354A1 (en) | 2011-04-18 | 2014-04-17 | Garvan Institute Of Medical Research | Method of Diagnosing Cancer |
WO2012151111A1 (en) | 2011-05-04 | 2012-11-08 | Htg Molecular Diagnostics, Inc. | Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements |
WO2012158967A1 (en) | 2011-05-17 | 2012-11-22 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
WO2012165943A1 (en) | 2011-05-27 | 2012-12-06 | Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patiëntenzorg | A method of analysing a blood sample of a subject for the presence of an infectious disease marker |
WO2012169887A2 (en) | 2011-06-06 | 2012-12-13 | Stichting Katholieke Universiteit, More Particularly The Radboud University Nijmegen Medical Centre | Use of new markers in a diagnostic assay for determining severity of rsv infection |
US9364532B2 (en) | 2011-06-08 | 2016-06-14 | Children's Hospital Of Eastern Ontario Research Institute Inc. | Compositions and methods for glioblastoma treatment |
EP2723365A1 (en) | 2011-06-21 | 2014-04-30 | Oncofactor Corporation | Compositions and methods for the therapy and diagnosis of cancer |
RS55716B1 (sr) | 2011-06-28 | 2017-07-31 | Oxford Biotherapeutics Ltd | Terapeutski i dijagnostički cilj |
WO2013006684A1 (en) | 2011-07-05 | 2013-01-10 | The Gov. Of The U.S.A. As Represented By The Secretary Of The Dept. Of Health And Human Services. | Hiv-1 genotyping assay for global surveillance of hiv-1 drug resistance |
AU2012281242B2 (en) | 2011-07-08 | 2016-12-22 | Keygene N.V. | Sequence based genotyping based on oligonucleotide ligation assays |
CN113215308A (zh) | 2011-07-15 | 2021-08-06 | 简.探针公司 | 检测人细小病毒核酸和甲型肝炎病毒核酸的组合物和方法 |
EP2742150A1 (en) | 2011-08-09 | 2014-06-18 | Vereniging voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek en Patiëntenzorg | A method of analysing a blood sample of a subject for the presence of a foetal disease or condition marker |
EP2746395B1 (en) | 2011-09-08 | 2017-11-29 | Kabushiki Kaisha DNAFORM | Primer set, method for amplifying target nucleic acid sequence using same, and method for detecting mutated nucleic acid using same |
US10040853B2 (en) | 2011-09-09 | 2018-08-07 | Fred Hutchinson Cancer Research Center | Methods and compositions involving NKG2D inhibitors and cancer |
JP2014530011A (ja) | 2011-09-28 | 2014-11-17 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company | Stec細菌の配列ならびに検出および特徴決定のためのそれらの使用 |
WO2013049535A2 (en) | 2011-09-30 | 2013-04-04 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Influenza vaccine |
US10876160B2 (en) | 2011-10-31 | 2020-12-29 | Eiken Kagaku Kabushiki Kaisha | Method for detecting target nucleic acid |
CN103917665B (zh) | 2011-11-09 | 2016-11-16 | 纳幕尔杜邦公司 | 用于沙门氏菌检测的序列和它们的用法 |
US9890431B2 (en) | 2011-11-15 | 2018-02-13 | Université Libre de Bruxelles | Streptococcus pneumoniae detection in blood |
US8748097B1 (en) | 2011-12-02 | 2014-06-10 | President And Fellows Of Harvard College | Identification of agents for treating calcium disorders and uses thereof |
US9701959B2 (en) | 2012-02-02 | 2017-07-11 | Invenra Inc. | High throughput screen for biologically active polypeptides |
CA2865977A1 (en) | 2012-02-29 | 2013-09-06 | Dow Agrosciences Llc | Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics |
US9428813B2 (en) | 2012-03-26 | 2016-08-30 | The United States Of America, As Represented By The Secretary, Dept. Of Health & Human Services | DNA methylation analysis for the diagnosis, prognosis and treatment of adrenal neoplasms |
US9566329B2 (en) | 2012-04-06 | 2017-02-14 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Live, attenuated rubella vector to express vaccine antigens |
WO2013162828A1 (en) | 2012-04-27 | 2013-10-31 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Use of cpg oligonucleotides co-formulated with an antibiotic to accelarate wound healing |
ES2683979T3 (es) | 2012-05-02 | 2018-10-01 | Ibis Biosciences, Inc. | Secuenciación de ADN |
EP2844772B1 (en) | 2012-05-02 | 2018-07-11 | Ibis Biosciences, Inc. | Dna sequencing |
US9803251B2 (en) | 2012-05-03 | 2017-10-31 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods of detecting influenza virus |
EP4001436B1 (en) | 2012-05-11 | 2024-03-13 | Clinical Genomics Pty. Ltd. | Diagnostic gene marker panel |
DK2850212T3 (da) | 2012-05-18 | 2020-03-23 | Clinical Genomics Pty Ltd | Fremgangsmåde til screening for colorektal cancer |
US9394574B2 (en) | 2012-06-12 | 2016-07-19 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods for detecting Legionella nucleic acids in a sample |
US9631243B2 (en) | 2012-06-18 | 2017-04-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods and compositions for identifying JC virus |
GB201213652D0 (en) | 2012-08-01 | 2012-09-12 | Oxford Biotherapeutics Ltd | Therapeutic and diagnostic target |
EP2882868B1 (en) | 2012-08-08 | 2019-07-31 | H. Hoffnabb-La Roche Ag | Increasing dynamic range for identifying multiple epitopes in cells |
CA2883219C (en) | 2012-08-30 | 2020-12-29 | Gen-Probe Incorporated | Multiphase nucleic acid amplification |
WO2014043518A1 (en) | 2012-09-14 | 2014-03-20 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Brachyury protein, non-poxvirus non-yeast vectors encoding brachyury protein, and their use |
WO2014045254A2 (en) | 2012-09-23 | 2014-03-27 | Erasmus University Medical Center Rotterdam | Human betacoronavirus lineage c and identification of n-terminal dipeptidyl peptidase as its virus receptor |
IN2015DN02893A (zh) | 2012-10-04 | 2015-09-11 | Univ Leland Stanford Junior | |
US9181583B2 (en) | 2012-10-23 | 2015-11-10 | Illumina, Inc. | HLA typing using selective amplification and sequencing |
ES2730690T3 (es) | 2012-12-07 | 2019-11-12 | Suppremol Gmbh | Estratificación y tratamiento de pacientes que padecen púrpura trombocitopénica idiopática |
US9775895B2 (en) | 2012-12-12 | 2017-10-03 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | HIV therapeutics and methods of making and using same |
EP2935908B1 (en) | 2012-12-21 | 2019-08-14 | PerkinElmer Health Sciences, Inc. | Fluidic circuits and related manufacturing methods |
KR20150096788A (ko) | 2012-12-21 | 2015-08-25 | 마이크로닉스 인코포레이티드. | 마이크로 유체공학 용도를 위한 저탄성 막 |
KR20150097764A (ko) | 2012-12-21 | 2015-08-26 | 마이크로닉스 인코포레이티드. | 휴대형 형광 검출 시스템 및 미량분석 카트리지 |
US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
US10125373B2 (en) | 2013-01-22 | 2018-11-13 | Arizona Board Of Regents On Behalf Of Arizona State University | Geminiviral vector for expression of rituximab |
US10077475B2 (en) | 2013-01-24 | 2018-09-18 | California Institute Of Technology | FRET-based analytes detection and related methods and systems |
CA2897474A1 (en) | 2013-01-24 | 2014-07-31 | California Institute Of Technology | Chromophore-based characterization and detection methods |
EP3323884A1 (en) | 2013-02-01 | 2018-05-23 | The United States Of America as Represented by the Secretary, Department of Health an Human Service | Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs) |
GB201302447D0 (en) | 2013-02-12 | 2013-03-27 | Oxford Biotherapeutics Ltd | Therapeutic and diagnostic target |
WO2014126921A1 (en) | 2013-02-12 | 2014-08-21 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Monoclonal antibodies that neutralize norovirus |
CA2901501C (en) | 2013-02-21 | 2023-03-07 | Children's Hospital Of Eastern Ontario Research Institute Inc. | Vaccine composition |
CA2907484C (en) | 2013-03-13 | 2021-06-29 | Illumina, Inc. | Methods and systems for aligning repetitive dna elements |
AU2013382098B2 (en) | 2013-03-13 | 2019-02-07 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
WO2014152155A1 (en) | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Massively multiplexed rna sequencing |
WO2014140167A1 (en) | 2013-03-14 | 2014-09-18 | Dsm Ip Assets B.V. | Cell wall deconstruction enzymes of malbranchea cinnamomea and uses thereof |
WO2014140165A1 (en) | 2013-03-14 | 2014-09-18 | Dsm Ip Assets B.V. | Cell wall deconstruction enzymes of paecilomyces byssochlamydoides and uses thereof |
AU2014237563B2 (en) | 2013-03-15 | 2020-06-11 | Becton, Dickinson And Company | Detection of neisseria gonorrhoeaes |
AU2014262710B2 (en) | 2013-05-07 | 2019-09-12 | Perkinelmer Health Sciences, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
US10386377B2 (en) | 2013-05-07 | 2019-08-20 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
AU2014262726B2 (en) | 2013-05-07 | 2019-09-19 | Perkinelmer Health Sciences, Inc. | Device for preparation and analysis of nucleic acids |
EP2994163B1 (en) | 2013-05-09 | 2019-08-28 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Single-domain vhh antibodies directed to norovirus gi.1 and gii.4 and their use |
GB201309421D0 (en) | 2013-05-24 | 2013-07-10 | Imp Innovations Ltd | Polypeptides |
CA2920636A1 (en) | 2013-08-20 | 2015-02-23 | E. I. Du Pont De Nemours And Company | Sequences and their use for detection of salmonella enteritidis and/or salmonella typhimurium |
FI3533882T3 (fi) | 2013-10-15 | 2023-01-13 | Merkittävän histologisen yhteensopivuuskompleksin yksittäisen nukleotidin polymorfismeja | |
US10006097B2 (en) | 2013-10-22 | 2018-06-26 | The United States of America, as represented by the Secretry, Department of Health and Human Services | Compositions and methods for detection and discrimination of influenza viruses |
EP3080268A1 (en) | 2013-12-09 | 2016-10-19 | Illumina, Inc. | Methods and compositions for targeted nucleic acid sequencing |
DK3083994T3 (da) | 2013-12-20 | 2021-09-13 | Illumina Inc | Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver |
CN113504375B (zh) | 2014-01-07 | 2024-01-09 | 生物蛋白有限公司 | 靶向直向同源物的蛋白 |
US10398772B2 (en) | 2014-01-08 | 2019-09-03 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Ras pathways as markers of protection against HIV and methods to improve vaccine efficacy |
US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
WO2015108663A1 (en) | 2014-01-16 | 2015-07-23 | Illumina, Inc. | Amplicon preparation and sequencing on solid supports |
EP3105254B1 (en) | 2014-02-14 | 2019-11-20 | Symvivo Corporation | Hybrid proteins and uses thereof |
WO2015127201A1 (en) | 2014-02-21 | 2015-08-27 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Hiv-2 nucleic acids and methods of detection |
US20170044631A1 (en) | 2014-04-14 | 2017-02-16 | The Unite States of America, as represented by the Secretary, Dept. of Health and Human Services | Methods for rapid detection and identification of viral nucleic acids |
US20150353989A1 (en) | 2014-06-09 | 2015-12-10 | Illumina Cambridge Limited | Sample preparation for nucleic acid amplification |
CN106879252B (zh) | 2014-06-10 | 2020-07-17 | 德克斯特里蒂诊断公司 | 用于收集和稳定生物样品的装置及方法 |
KR20170016915A (ko) | 2014-06-11 | 2017-02-14 | 마이크로닉스 인코포레이티드. | 핵산의 분석을 위한 통합 검정 대조군을 갖는 마이크로 유체 공학적 카트리지 및 장치 |
GB201410420D0 (en) | 2014-06-11 | 2014-07-23 | Illumina Cambridge Ltd | Methods for estimating cluster numbers |
CA2952058A1 (en) | 2014-06-13 | 2015-12-17 | Illumina Cambridge Limited | Methods and compositions for preparing sequencing libraries |
WO2015199976A1 (en) | 2014-06-24 | 2015-12-30 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Target activated microdissection |
US10577603B2 (en) | 2014-06-30 | 2020-03-03 | Illumina, Inc. | Methods and compositions using one-sided transposition |
CN107075573A (zh) | 2014-07-30 | 2017-08-18 | 莫尔研究应用有限公司 | 用于急性淋巴细胞白血病的预后方法和治疗系统 |
ES2925224T3 (es) | 2014-07-31 | 2022-10-14 | Us Gov Health & Human Services | Anticuerpos monoclonales humanos contra EphA4 y su uso |
AU2015311911B2 (en) | 2014-09-03 | 2019-01-24 | Bioatla, Llc | Discovering and producing conditionally active biologic proteins in the same eukaryotic cell production hosts |
WO2016040602A1 (en) | 2014-09-11 | 2016-03-17 | Epicentre Technologies Corporation | Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv |
EP3194624B1 (en) | 2014-09-15 | 2022-02-16 | Garvan Institute of Medical Research | Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor |
KR102538753B1 (ko) | 2014-09-18 | 2023-05-31 | 일루미나, 인코포레이티드 | 핵산 서열결정 데이터를 분석하기 위한 방법 및 시스템 |
RU2709655C2 (ru) | 2014-10-17 | 2019-12-19 | Иллумина Кембридж Лимитед | Транспозиция с сохранением сцепления генов |
GB201419731D0 (en) | 2014-11-05 | 2014-12-17 | Illumina Cambridge Ltd | Sequencing from multiple primers to increase data rate and density |
EP3227462B1 (en) | 2014-12-01 | 2020-04-22 | The Broad Institute, Inc. | Method for in situ determination of nucleic acid proximity |
US10072070B2 (en) | 2014-12-05 | 2018-09-11 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Potent anti-influenza A neuraminidase subtype N1 antibody |
EP4242329A3 (en) | 2014-12-08 | 2023-10-25 | Berg LLC | Use of markers including filamin a in the diagnosis and treatment of prostate cancer |
AU2016219511B2 (en) | 2015-02-09 | 2020-11-12 | Research Development Foundation | Engineered immunoglobulin Fc polypeptides displaying improved complement activation |
WO2016134144A1 (en) | 2015-02-20 | 2016-08-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods and reagents for detecting ebola virus |
CN107614700A (zh) | 2015-03-11 | 2018-01-19 | 布罗德研究所有限公司 | 基因型和表型偶联 |
EP3783109B1 (en) | 2015-03-31 | 2024-05-29 | Illumina Cambridge Limited | Surface concatamerization of templates |
ES2971094T3 (es) | 2015-04-07 | 2024-06-03 | Polyskope Labs | Detección de uno o más patógenos |
CA3224392A1 (en) | 2015-04-24 | 2016-10-27 | Becton, Dickinson And Company | Multiplex detection of vulvovaginal candidiasis, trichomoniasis and bacterial vaginosis |
EP3878974A1 (en) | 2015-07-06 | 2021-09-15 | Illumina Cambridge Limited | Sample preparation for nucleic acid amplification |
WO2017040380A2 (en) | 2015-08-28 | 2017-03-09 | Research Development Foundation | Engineered antibody fc variants |
WO2017081205A1 (en) | 2015-11-10 | 2017-05-18 | Vilmorin & Cie | RESISTANCE TO ToLCNDV IN SQUASH |
EP3405559B1 (en) | 2016-01-21 | 2021-11-17 | T2 Biosystems, Inc. | Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods |
US9771623B1 (en) | 2016-03-15 | 2017-09-26 | The Board Of Regents Of The University Of Texas System | Molecular typing system for flavivirus diagnostics |
SG11201805665UA (en) | 2016-03-28 | 2018-10-30 | Illumina Inc | Multi-plane microarrays |
CA3020581A1 (en) | 2016-04-14 | 2017-10-19 | T2 Biosystems, Inc. | Methods and systems for amplification in complex samples |
WO2017192589A1 (en) | 2016-05-02 | 2017-11-09 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Neutralizing antibodies to influenza ha and their use and identification |
WO2017205957A1 (en) | 2016-06-01 | 2017-12-07 | 9087-4405 Quebec Inc. | Remote access system and method for plant pathogen management |
WO2018005284A1 (en) | 2016-06-27 | 2018-01-04 | The United State Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods and compositions for influenza a virus subtyping |
ES2873723T3 (es) | 2016-07-22 | 2021-11-03 | Univ Oregon Health & Science | Colecciones de genoma completo de células individuales y métodos de indexación combinatoria para prepararlas |
JP7086984B2 (ja) | 2016-12-06 | 2022-06-20 | オレゴン ステイト ユニバーシティ | Streptomyces fungicidicusの遺伝子組換え株におけるエンデュラシジンの産生を増強するための組成物及び方法 |
CN110462062A (zh) | 2017-01-26 | 2019-11-15 | 俄克拉荷马医学研究基金会 | 系统性红斑狼疮疾病活动性、强度和急性发作的生物标志物 |
EP4282986A3 (en) | 2017-03-24 | 2024-02-28 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying parainfluenza virus |
ES2929837T3 (es) | 2017-04-23 | 2022-12-02 | Illumina Cambridge Ltd | Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados |
SG11201909394PA (en) | 2017-04-23 | 2019-11-28 | Illumina Inc | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
WO2018197945A1 (en) | 2017-04-23 | 2018-11-01 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
US10995104B2 (en) | 2017-05-30 | 2021-05-04 | Roche Molecular System, Inc. | Catalysts for reversing formaldehyde adducts and crosslinks |
ES2898250T3 (es) | 2017-06-07 | 2022-03-04 | Univ Oregon Health & Science | Bibliotecas de genoma completo de células individuales para secuenciación de la metilación |
WO2018232028A1 (en) | 2017-06-14 | 2018-12-20 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Detection of blaimp antibacterial resistance genes |
GB201710838D0 (en) | 2017-07-05 | 2017-08-16 | Ucl Business Plc | Bispecific antibodies |
EP3662482A1 (en) | 2017-07-31 | 2020-06-10 | Illumina Inc. | Sequencing system with multiplexed biological sample aggregation |
WO2019028166A1 (en) | 2017-08-01 | 2019-02-07 | Illumina, Inc. | HYDROGEL BALLS FOR NUCLEOTIDE SEQUENCING |
CN111094595A (zh) | 2017-08-11 | 2020-05-01 | 简·探针公司 | 用于检测金黄色葡萄球菌的组合物和方法 |
US11733248B2 (en) | 2017-09-25 | 2023-08-22 | Fred Hutchinson Cancer Center | High efficiency targeted in situ genome-wide profiling |
US20200255828A1 (en) | 2017-10-04 | 2020-08-13 | The Broad Institute, Inc. | Methods and compositions for altering function and structure of chromatin loops and/or domains |
AU2019220559B2 (en) | 2018-02-13 | 2021-10-28 | Illumina, Inc. | DNA sequencing using hydrogel beads |
EP3775278A1 (en) | 2018-04-02 | 2021-02-17 | Illumina Inc. | Compositions and methods for making controls for sequence-based genetic testing |
CN111051525A (zh) | 2018-04-20 | 2020-04-21 | 伊鲁米纳公司 | 包封单细胞的方法、包封的单细胞及其用途 |
RU2744175C1 (ru) | 2018-05-17 | 2021-03-03 | Иллумина, Инк. | Высокопроизводительное секвенирование одиночной клетки со сниженной ошибкой амплификации |
AU2019282158B2 (en) | 2018-06-04 | 2021-08-12 | Illumina, Inc. | High-throughput single-cell transcriptome libraries and methods of making and of using |
GB2597567B (en) | 2018-06-13 | 2022-11-23 | Gen Probe Inc | Compositions and methods for detecting group B streptococcus nucleic acid |
AU2019309520A1 (en) | 2018-07-27 | 2021-03-11 | Aperta Biosciences, Llc | Spinosyn formulations for treatment of demodex-induced ocular and facial conditions |
WO2020028631A1 (en) | 2018-08-01 | 2020-02-06 | Gen-Probe Incorporated | Compositions and methods for detecting nucleic acids of epstein-barr virus |
CA3108105A1 (en) | 2018-08-08 | 2020-02-13 | Gen-Probe Incorporated | Compositions, methods and kits for detecting mycoplasma genitalium |
EP3841224A1 (en) | 2018-08-21 | 2021-06-30 | Gen-Probe Incorporated | Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus |
US20210324452A1 (en) | 2018-09-06 | 2021-10-21 | Hygiena, Llc | Sequences and their use for detection and characterization of escherichia coli serotype o157:h7 |
CA3112651A1 (en) | 2018-09-27 | 2020-04-02 | Gen-Probe Incorporated | Compositions and methods for detecting bordetella pertussis and bordetella parapertussis nucleic acid |
CA3116895A1 (en) | 2018-10-22 | 2020-04-30 | Gen-Probe Incorporated | Compositions and methods for amplifying, detecting or quantifying human polyomavirus bk virus |
WO2020086843A1 (en) | 2018-10-26 | 2020-04-30 | Illumina, Inc. | Modulating polymer beads for dna processing |
US20220195504A1 (en) | 2018-11-30 | 2022-06-23 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
JP2022522917A (ja) | 2019-03-01 | 2022-04-21 | イルミナ インコーポレイテッド | ハイスループット単一核及び単一細胞ライブラリー、並びに製造及び使用方法 |
US20220098647A1 (en) | 2019-03-22 | 2022-03-31 | Gen-Probe Incorporated | Compositions and Methods for Detecting Group A Streptococcus |
WO2020205491A1 (en) | 2019-04-05 | 2020-10-08 | Hygiena, Llc | Sequences and their use for detection and characterization of genus cronobacter |
WO2020210754A1 (en) | 2019-04-10 | 2020-10-15 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Computational filtering of methylated sequence data for predictive modeling |
KR20220015394A (ko) | 2019-04-30 | 2022-02-08 | 라리마 테라퓨틱스, 인코포레이티드 | 프라탁신 대체 요법의 유효성 결정을 위한 프라탁신 민감성 마커 |
CN113260711A (zh) | 2019-07-12 | 2021-08-13 | Illumina剑桥有限公司 | 使用固定在固体载体上的crispr/cas9制备核酸测序文库的组合物和方法 |
US20220127596A1 (en) | 2019-07-12 | 2022-04-28 | lllumina Cambridge Limited | Nucleic Acid Library Preparation Using Electrophoresis |
EP4018004A1 (en) | 2019-08-23 | 2022-06-29 | Gen-Probe Incorporated | Compositions, methods and kits for detecting treponema pallidum |
EP4025715A1 (en) | 2019-09-05 | 2022-07-13 | Gen-Probe Incorporated | Detection of chlamydia trachomatis nucleic acid variants |
US11844800B2 (en) | 2019-10-30 | 2023-12-19 | Massachusetts Institute Of Technology | Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia |
CA3134746A1 (en) | 2019-12-19 | 2021-06-24 | Illumina, Inc. | High-throughput single-cell libraries and methods of making and of using |
US20230227896A1 (en) | 2020-01-16 | 2023-07-20 | Dnae Diagnostics Limited | Compositions, Kits and Methods for Isolating Target Polynucleotides |
CA3169966A1 (en) | 2020-03-03 | 2021-09-10 | John E. Gill | Methods for assembling nucleic acids |
CA3176615A1 (en) | 2020-03-30 | 2021-10-07 | Illumina, Inc. | Methods and compositions for preparing nucleic acid libraries |
EP4150065A2 (en) | 2020-05-12 | 2023-03-22 | Illumina Inc | Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase |
WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
KR20230037503A (ko) | 2020-06-30 | 2023-03-16 | 일루미나, 인코포레이티드 | 무흔적 dna를 생성하기 위한 촉매적으로 제어된 합성에 의한 서열분석 |
AU2021308095A1 (en) | 2020-07-17 | 2023-03-09 | Gen-Probe Incorporated | Detection of macrolide-resistant mycoplasma genitalium |
KR20230041725A (ko) | 2020-08-06 | 2023-03-24 | 일루미나, 인코포레이티드 | 비드-연결된 트랜스포좀을 사용한 rna 및 dna 시퀀싱 라이브러리의 제작 |
AU2021329302A1 (en) | 2020-08-18 | 2023-02-16 | Illumina Cambridge Limited | Sequence-specific targeted transposition and selection and sorting of nucleic acids |
WO2022047359A1 (en) | 2020-08-31 | 2022-03-03 | Berg Llc | Protein biomarkers for pancreatic cancer |
EP4211261A1 (en) | 2020-09-11 | 2023-07-19 | Illumina Cambridge Limited | Methods of enriching a target sequence from a sequencing library using hairpin adaptors |
KR20230097143A (ko) | 2020-11-05 | 2023-06-30 | 백톤 디킨슨 앤드 컴퍼니 | 박테리아 호흡기 병원체의 멀티플렉스 검출 |
WO2022095922A1 (en) | 2020-11-05 | 2022-05-12 | Becton, Dickinson And Company | Rapid identification and typing of vibrio parahaemolyticus |
AU2021374695A1 (en) | 2020-11-05 | 2023-07-13 | Becton, Dickinson And Company | Multiplex detection and typing of vibrio cholerae |
KR20230137394A (ko) | 2021-02-04 | 2023-10-04 | 일루미나, 인코포레이티드 | 트랜스포좀 결합된 비드 상의 긴 인덱싱된-연결된 판독 생성 |
IL307195A (en) | 2021-03-29 | 2023-11-01 | Illumina Inc | Improved methods of library preparation |
IL307177A (en) | 2021-03-29 | 2023-11-01 | Illumina Inc | Compounds and methods for assessing DNA damage in the library and normalizing amplicon size bias |
MX2023010495A (es) | 2021-03-30 | 2023-09-18 | Illumina Inc | Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos. |
CA3211172A1 (en) | 2021-03-31 | 2022-10-06 | Illumina, Inc. | Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction |
CA3214819A1 (en) | 2021-04-06 | 2022-10-13 | Guisong WANG | Protein markers for estrogen receptor (er)-positive luminal a(la)-like and luminal b1 (lb1)-like breast cancer |
CA3214821A1 (en) | 2021-04-06 | 2022-10-13 | Guisong WANG | Protein markers for estrogen receptor (er)-positive-like and estrogen receptor (er)-negative-like breast cancer |
EP4320443A1 (en) | 2021-04-06 | 2024-02-14 | BPGbio, Inc. | Protein markers for the prognosis of breast cancer progression |
CA3220932A1 (en) | 2021-07-01 | 2023-01-05 | Gen-Probe Incorporated | Enzyme formulations and reaction mixtures for nucleic acid amplification |
EP4373965A1 (en) | 2021-07-21 | 2024-05-29 | DNAe Diagnostics Limited | Method and system comprising a cartridge for sequencing target polynucleotides |
GB202110485D0 (en) | 2021-07-21 | 2021-09-01 | Dnae Diagnostics Ltd | Compositions, kits and methods for sequencing target polynucleotides |
GB202110479D0 (en) | 2021-07-21 | 2021-09-01 | Dnae Diagnostics Ltd | Compositions, kits and methods for sequencing target polynucleotides |
EP4382621A2 (en) | 2021-07-27 | 2024-06-12 | Gen-Probe Incorporated | Compositions and methods for detecting c. coli and at least one of salmonella, c. jejuni, shigella, and stec |
US20230193406A1 (en) | 2021-09-22 | 2023-06-22 | Herbalife International Of America, Inc. | Methods and compositions for processing botanical materials |
WO2023196937A1 (en) | 2022-04-06 | 2023-10-12 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for monitoring frataxin replacement therapy |
WO2023196572A1 (en) | 2022-04-07 | 2023-10-12 | Illumina Singapore Pte. Ltd. | Altered cytidine deaminases and methods of use |
EP4282980A1 (en) | 2022-05-23 | 2023-11-29 | Mobidiag Oy | Methods for amplifying a nucleic acid |
WO2023230531A1 (en) | 2022-05-24 | 2023-11-30 | Lunglife Ai, Inc. | Methods for detecting circulating genetically abnormal cells |
WO2023240201A1 (en) | 2022-06-08 | 2023-12-14 | Larimar Therapeutics, Inc. | Frataxin-sensitive markers for monitoring progression and treatment of leigh syndrome |
US20240010742A1 (en) | 2022-06-10 | 2024-01-11 | Research Development Foundation | Engineered fcriib selective igg1 fc variants and uses thereof |
WO2024030985A1 (en) | 2022-08-04 | 2024-02-08 | Abbott Laboratories | Assays for detecting monkeypox virus |
WO2024054924A1 (en) | 2022-09-08 | 2024-03-14 | Gen-Probe Incorporated | Method of detecting nucleic acid analytes using dual-specificity primers |
WO2024073047A1 (en) | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Cytidine deaminases and methods of use in mapping modified cytosine nucleotides |
WO2024069581A1 (en) | 2022-09-30 | 2024-04-04 | Illumina Singapore Pte. Ltd. | Helicase-cytidine deaminase complexes and methods of use |
WO2024073043A1 (en) | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Methods of using cpg binding proteins in mapping modified cytosine nucleotides |
WO2024081776A1 (en) | 2022-10-13 | 2024-04-18 | Gen-Probe Incorporated | Cellularity control for nucleic acid assays |
EP4389887A1 (en) | 2022-12-21 | 2024-06-26 | Mobidiag Oy | Isolating and lysing cells |
EP4389911A1 (en) | 2022-12-21 | 2024-06-26 | Mobidiag Oy | Methods and systems for isolating an analyte |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1222680A (en) * | 1983-07-05 | 1987-06-09 | Nanibhushan Dattagupta | Testing dna samples for particular nucleotide sequences |
FI71768C (fi) * | 1984-02-17 | 1987-02-09 | Orion Yhtymae Oy | Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning. |
US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
IL79112A (en) * | 1985-06-13 | 1992-01-15 | Abbott Lab | Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay |
CA1339653C (en) * | 1986-02-25 | 1998-02-03 | Larry J. Johnson | Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps |
GB8608269D0 (en) * | 1986-04-04 | 1986-05-08 | Brown J J | Ice-maker |
GB8612087D0 (en) * | 1986-05-19 | 1986-06-25 | Ici Plc | Hybridisation probes |
US5449602A (en) * | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
CA1341584C (en) * | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
-
1988
- 1988-12-09 AU AU26755/88A patent/AU622426B2/en not_active Ceased
- 1988-12-09 CA CA000585462A patent/CA1323293C/en not_active Expired - Fee Related
- 1988-12-10 CN CN88109238A patent/CN1035133A/zh active Pending
- 1988-12-12 JP JP63313658A patent/JPH0634759B2/ja not_active Expired - Fee Related
- 1988-12-12 AT AT88311741T patent/ATE96848T1/de not_active IP Right Cessation
- 1988-12-12 BR BR888806539A patent/BR8806539A/pt not_active IP Right Cessation
- 1988-12-12 ES ES88311741T patent/ES2061694T3/es not_active Expired - Lifetime
- 1988-12-12 EP EP88311741A patent/EP0320308B1/en not_active Expired - Lifetime
- 1988-12-12 DE DE88311741T patent/DE3885422T2/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JPH0634759B2 (ja) | 1994-05-11 |
EP0320308B1 (en) | 1993-11-03 |
AU2675588A (en) | 1989-06-15 |
CA1323293C (en) | 1993-10-19 |
ATE96848T1 (de) | 1993-11-15 |
AU622426B2 (en) | 1992-04-09 |
EP0320308A3 (en) | 1990-06-06 |
JPH022934A (ja) | 1990-01-08 |
ES2061694T3 (es) | 1994-12-16 |
DE3885422T2 (de) | 1994-04-28 |
EP0320308A2 (en) | 1989-06-14 |
BR8806539A (pt) | 1989-08-22 |
DE3885422D1 (de) | 1993-12-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1035133A (zh) | 用与模板有关的核酸探测剂改组的测定方法 | |
Walker et al. | Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. | |
JP3514630B2 (ja) | 核酸配列の増幅および検出 | |
US6117634A (en) | Nucleic acid sequencing and mapping | |
US5545540A (en) | Isothermal, magnetic particle-mediated acid amplification | |
US5223414A (en) | Process for nucleic acid hybridization and amplification | |
US5210015A (en) | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase | |
US5491225A (en) | PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays | |
US5536648A (en) | Amplified DNA assay using a double stranded DNA binding protein | |
WO1992001813A1 (en) | Circular extension for generating multiple nucleic acid complements | |
EP0466520A1 (en) | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers | |
AU633036B2 (en) | Amplified dna assay | |
JPH06209799A (ja) | 鳥型結核菌、ミコバクテリウム・イントラセルレアおよびパラ結核菌に対するプローブ | |
JPH09500539A (ja) | 核酸のリンクされた線形増幅 | |
CA2199213C (en) | Amplifying and detecting target nucleic acids using a post amplification incubation step | |
JP2002514909A (ja) | ハイブリダイゼーション特異性を増強するための組成物および方法 | |
JPH1118785A (ja) | ナイセリア・コノルホエア−特異的オリゴヌクレオチド | |
JP2928992B2 (ja) | Dna及び/又はrnaを特異的に増幅し検出する方法 | |
EP1117826A1 (en) | Oligonucleotide primers that destabilize non-specific duplex formation and uses thereof | |
JP4782784B2 (ja) | 病原性大腸菌の検出および識別のためのdna配列 | |
JPH0852000A (ja) | 核酸の同時増幅方法 | |
Thomas | PCR based diagnostic techniques-Winter school on recent advances in diagnosis and management of diseases in mariculture, 7th to 27th November 2002, Course Manual | |
Myint | The application of molecular biology to the diagnosis of infectious disease | |
BRPI0204901B1 (pt) | método para amplificar e identificar sequências de nucleotídeos e kit para uso na amplificação e identificação de sequências de nucleotídeos |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C01 | Deemed withdrawal of patent application (patent law 1993) | ||
WD01 | Invention patent application deemed withdrawn after publication |