BR112013004691A2 - construto de dna de ativação gênica marcada de milho, população marcada de plantas de milho, método para geração da mesma e método para gerar uma planta de milho marcada - Google Patents

construto de dna de ativação gênica marcada de milho, população marcada de plantas de milho, método para geração da mesma e método para gerar uma planta de milho marcada

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Publication number
BR112013004691A2
BR112013004691A2 BR112013004691A BR112013004691A BR112013004691A2 BR 112013004691 A2 BR112013004691 A2 BR 112013004691A2 BR 112013004691 A BR112013004691 A BR 112013004691A BR 112013004691 A BR112013004691 A BR 112013004691A BR 112013004691 A2 BR112013004691 A2 BR 112013004691A2
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Brazil
Prior art keywords
marked
generating
maize
gene activation
plants
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BR112013004691A
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English (en)
Inventor
Ry Wagner D
P Davies John
S Reddy Vaka
M Ainley William
L Liu Xing
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Dow Agrosciences Llc
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Publication date
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Publication of BR112013004691A2 publication Critical patent/BR112013004691A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

plataforma de ativação gênica marcada para milho, e populações e plantas marcadas resultantes. a presente invenção refere-se a um construto de ativação gênica marcada para milho, as populações e plantas marcadas resultantes. em um exemplo, um cosntruto de dna de ativação gênica marcada inclui uma sequência codificante para uma transposase, um repórter detectável (tal como os genes reguladores de antocianina b-peru e c1) e um cassete de t-dna transponível não autônomo. por exemplo, o cassete de t-dna transponível é inserido na região de codificação do repórter detectável de modo que os genes b-peru e c1 expressem antocianinas em uma célula contendo o construto de dna de ativação gênica marcada de milho apenas mediante excisão do cassete transponível. os métodos para gerar a população marcada de plantas de milho incluem transformar uma célula ou tecido vegetal de milho com os construtos apresentados.
BR112013004691A 2010-08-30 2011-08-29 construto de dna de ativação gênica marcada de milho, população marcada de plantas de milho, método para geração da mesma e método para gerar uma planta de milho marcada BR112013004691A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40257410P 2010-08-30 2010-08-30
PCT/US2011/049535 WO2012030714A2 (en) 2010-08-30 2011-08-29 Activation tagging platform for maize, and resultant tagged population and plants

Publications (1)

Publication Number Publication Date
BR112013004691A2 true BR112013004691A2 (pt) 2017-11-21

Family

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BR112013004691A BR112013004691A2 (pt) 2010-08-30 2011-08-29 construto de dna de ativação gênica marcada de milho, população marcada de plantas de milho, método para geração da mesma e método para gerar uma planta de milho marcada

Country Status (9)

Country Link
US (2) US8912393B2 (pt)
EP (1) EP2611924B1 (pt)
CN (1) CN103221543B (pt)
AR (1) AR082785A1 (pt)
AU (1) AU2011296212B2 (pt)
BR (1) BR112013004691A2 (pt)
CA (1) CA2809644C (pt)
IL (2) IL224934A (pt)
WO (1) WO2012030714A2 (pt)

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TW201527313A (zh) * 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（二）
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TW201527312A (zh) 2013-12-31 2015-07-16 Dow Agrosciences Llc 新穎玉米泛素啓動子（一）
JP2018529652A (ja) 2015-08-17 2018-10-11 ダウ アグロサイエンシィズ エルエルシー 操作されたｃｒｙ６ａ殺虫性タンパク質
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Publication number Publication date
US20150096080A1 (en) 2015-04-02
AU2011296212A1 (en) 2013-03-14
US9896692B2 (en) 2018-02-20
AR082785A1 (es) 2013-01-09
CN103221543A (zh) 2013-07-24
CA2809644C (en) 2020-06-30
US20120060238A1 (en) 2012-03-08
AU2011296212B2 (en) 2015-06-11
CA2809644A1 (en) 2012-03-08
WO2012030714A3 (en) 2012-04-26
IL224934A (en) 2016-11-30
CN103221543B (zh) 2016-10-05
EP2611924A2 (en) 2013-07-10
US8912393B2 (en) 2014-12-16
IL248366A0 (en) 2016-11-30
WO2012030714A2 (en) 2012-03-08
EP2611924B1 (en) 2019-03-06

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