BR112013000267B8 - Métodos de introdução de uma molécula de cassete de ácido nucleico em uma célula vegetal e de introgressão de um traço em uma planta mediados por nanopartícula de ponto quântico - Google Patents

Métodos de introdução de uma molécula de cassete de ácido nucleico em uma célula vegetal e de introgressão de um traço em uma planta mediados por nanopartícula de ponto quântico

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Publication number
BR112013000267B8
BR112013000267B8 BR112013000267A BR112013000267A BR112013000267B8 BR 112013000267 B8 BR112013000267 B8 BR 112013000267B8 BR 112013000267 A BR112013000267 A BR 112013000267A BR 112013000267 A BR112013000267 A BR 112013000267A BR 112013000267 B8 BR112013000267 B8 BR 112013000267B8
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BR
Brazil
Prior art keywords
plant
nucleic acid
methods
cell
acid cassette
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BR112013000267A
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English (en)
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BR112013000267A2 (pt
BR112013000267B1 (pt
Inventor
Y Yau Kerrm
Pon Samuel Jayakumar
G Burroughs Frank
Chary Samboju Narasimha
R Webb Steven
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Dow Agrosciences Llc
Corteva Agriscience Llc
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Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR112013000267A2 publication Critical patent/BR112013000267A2/pt
Publication of BR112013000267B1 publication Critical patent/BR112013000267B1/pt
Publication of BR112013000267B8 publication Critical patent/BR112013000267B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PRODUÇÃO DE CASSETE DE DNA LINEAR FUNCIONALIZADO E DISTRIBUIÇÃO MEDIADA POR PONTO QUÂNTICO/NANOPARTÍCULA EM PLANTAS. A presente invenção refere-se a métodos para introdução de uma molécula de cassete de ácido nucleico linear funcionalizada de interesse em uma célula vegetal compreendendo uma parede celular que incluem o uso de nanopartículas. Em algumas modalidades, a célula compreendendo uma parede celular é uma célula vegetal cultivada. Os métodos incluem modificar geneticamente ou de outra maneira células vegetais e para tratamento ou prevenção de doença em qualquer planta, especialmente plantas de cultura. Plantas transgênicas incluem uma molécula de ácido nucleico de interesse produzida pela regeneração de plantas inteiras de células vegetais transformadas com moléculas de cassete de ácidos nucleicos lineares funcionalizadas.
BR112013000267A 2010-07-07 2011-07-07 Métodos de introdução de uma molécula de cassete de ácido nucleico em uma célula vegetal e de introgressão de um traço em uma planta mediados por nanopartícula de ponto quântico BR112013000267B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36222210P 2010-07-07 2010-07-07
US61/362,222 2010-07-07
US2012006439 2012-01-12

Publications (3)

Publication Number Publication Date
BR112013000267A2 BR112013000267A2 (pt) 2016-05-24
BR112013000267B1 BR112013000267B1 (pt) 2020-10-13
BR112013000267B8 true BR112013000267B8 (pt) 2022-06-28

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BR112013000267A BR112013000267B8 (pt) 2010-07-07 2011-07-07 Métodos de introdução de uma molécula de cassete de ácido nucleico em uma célula vegetal e de introgressão de um traço em uma planta mediados por nanopartícula de ponto quântico

Country Status (12)

Country Link
US (1) US8575424B2 (pt)
EP (1) EP2591115B1 (pt)
JP (3) JP6418738B2 (pt)
KR (1) KR20130124296A (pt)
CN (1) CN103052713A (pt)
AU (1) AU2011274807B2 (pt)
BR (1) BR112013000267B8 (pt)
CA (1) CA2804164C (pt)
IL (1) IL223980B (pt)
UA (1) UA112758C2 (pt)
WO (1) WO2012006439A2 (pt)
ZA (1) ZA201300167B (pt)

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US20130145488A1 (en) * 2011-12-06 2013-06-06 Iowa State University Research Foundation, Inc. Mesoporous silica nanoparticles suitable for co-delivery
CN102750333B (zh) * 2012-05-31 2014-05-07 华中科技大学 一种用于提取半导体纳米结构特征尺寸的方法
CA2886787A1 (en) 2012-10-05 2014-04-10 Dow Agrosciences Llc Use of cry1ea in combinations for management of resistant fall armyworm insects
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Also Published As

Publication number Publication date
JP2017140036A (ja) 2017-08-17
CA2804164A1 (en) 2012-01-12
AU2011274807B2 (en) 2015-04-30
JP2013529933A (ja) 2013-07-25
JP6418738B2 (ja) 2018-11-07
IL223980B (en) 2018-02-28
BR112013000267A2 (pt) 2016-05-24
RU2013104997A (ru) 2014-08-20
WO2012006439A2 (en) 2012-01-12
AU2011274807A1 (en) 2013-01-31
WO2012006439A3 (en) 2012-03-29
CA2804164C (en) 2019-10-22
EP2591115A2 (en) 2013-05-15
KR20130124296A (ko) 2013-11-13
ZA201300167B (en) 2014-03-26
EP2591115B1 (en) 2018-12-05
US20120023620A1 (en) 2012-01-26
BR112013000267B1 (pt) 2020-10-13
JP6891215B2 (ja) 2021-06-18
JP2019146580A (ja) 2019-09-05
CN103052713A (zh) 2013-04-17
EP2591115A4 (en) 2014-01-15
UA112758C2 (uk) 2016-10-25
US8575424B2 (en) 2013-11-05

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 07/07/2011, OBSERVADAS AS CONDICOES LEGAIS.

B25D Requested change of name of applicant approved

Owner name: CORTEVA AGRISCIENCE LLC (US)