BR112015004864B8 - Método para gerar uma célula de planta transgênica compreendendo um polinucleotídeo de interesse - Google Patents
Método para gerar uma célula de planta transgênica compreendendo um polinucleotídeo de interesseInfo
- Publication number
- BR112015004864B8 BR112015004864B8 BR112015004864A BR112015004864A BR112015004864B8 BR 112015004864 B8 BR112015004864 B8 BR 112015004864B8 BR 112015004864 A BR112015004864 A BR 112015004864A BR 112015004864 A BR112015004864 A BR 112015004864A BR 112015004864 B8 BR112015004864 B8 BR 112015004864B8
- Authority
- BR
- Brazil
- Prior art keywords
- etip
- plant
- transgenic plants
- plant cell
- invention describes
- Prior art date
Links
- 230000009261 transgenic effect Effects 0.000 title abstract 3
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- DWGXUDUOJPYAOR-UHFFFAOYSA-N ditophal Chemical compound CCSC(=O)C1=CC=CC(C(=O)SCC)=C1 DWGXUDUOJPYAOR-UHFFFAOYSA-N 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 108091033409 CRISPR Proteins 0.000 abstract 1
- 238000010354 CRISPR gene editing Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000005782 double-strand break Effects 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Botany (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
Abstract
ENRIQUECIMENTO DE CLASSIFICAÇÃO DE CÉLULAS ATIVADAS POR FLUORESCÊNCIA (FACS) PARA GERAR PLANTAS. A invenção descreve uma Plataforma de Integração de Transgenes Manipulados Geneticamente (ETIP) que pode ser inserida randomicamente ou em locais alvo nos genomas de plantas para facilitar a rápida seleção e detecção de um GOI que é perfeitamente direcionado (em ambas as extremidades 3' e 5') para o local genômico na ETIP. Um elemento da invenção é a introdução de rupturas de filamento duplo específicas na ETIP. Em algumas modalidades, a invenção descreve uma ETIP usando sítios fixadores da nuclease dedos-de-zinco, mas pode utilizar outras tecnologias de vetorização tais como meganucleases, TALs, CRISPRs, ou zíperes de leucina. Também estão descritas composições de plantas transgênicas, e método para produzir plantas transgênicas, onde o DNA doador ou de "payload" expressa um ou mais produtos de uma sequência de ácidos nucleicos exógena (por exemplo, proteína ou RNA) que fora estavelmente integrada em uma ETIP em uma célula vegetal. Nas modalidades, a ETIP facilita a testagem de genes candidatos e vetores de expressão vegetais da invenção através das fases de desenvolvimento.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261697890P | 2012-09-07 | 2012-09-07 | |
| US61/697,890 | 2012-09-07 | ||
| US14/020,694 | 2013-09-06 | ||
| US14/020,694 US20140075593A1 (en) | 2012-09-07 | 2013-09-06 | Fluorescence activated cell sorting (facs) enrichment to generate plants |
| PCT/US2013/058766 WO2014039970A1 (en) | 2012-09-07 | 2013-09-09 | Fluorescence activated cell sorting (facs) enrichment to generate plants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112015004864A2 BR112015004864A2 (pt) | 2017-11-21 |
| BR112015004864B1 BR112015004864B1 (pt) | 2022-10-25 |
| BR112015004864B8 true BR112015004864B8 (pt) | 2023-05-16 |
Family
ID=50234835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015004864A BR112015004864B8 (pt) | 2012-09-07 | 2013-09-09 | Método para gerar uma célula de planta transgênica compreendendo um polinucleotídeo de interesse |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20140075593A1 (pt) |
| EP (1) | EP2893024B1 (pt) |
| JP (1) | JP6362600B2 (pt) |
| KR (1) | KR102219621B1 (pt) |
| CN (2) | CN104736712A (pt) |
| AR (1) | AR092482A1 (pt) |
| AU (1) | AU2013312198B2 (pt) |
| BR (1) | BR112015004864B8 (pt) |
| CA (1) | CA2883800C (pt) |
| HK (1) | HK1210220A1 (pt) |
| IL (1) | IL237535B (pt) |
| IN (1) | IN2015DN01785A (pt) |
| MX (1) | MX367170B (pt) |
| RU (1) | RU2679510C2 (pt) |
| TW (1) | TWI670004B (pt) |
| WO (1) | WO2014039970A1 (pt) |
| ZA (1) | ZA201501736B (pt) |
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| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| JP6775953B2 (ja) | 2012-09-07 | 2020-10-28 | ダウ アグロサイエンシィズ エルエルシー | Ｆａｄ３性能座および標的化切断を誘導可能である対応する標的部位特異的結合タンパク質 |
| UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| CA2930015A1 (en) | 2013-11-07 | 2015-05-14 | Editas Medicine, Inc. | Crispr-related methods and compositions with governing grnas |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| AU2015355546B2 (en) | 2014-12-03 | 2021-10-14 | Agilent Technologies, Inc. | Guide RNA with chemical modifications |
| EP3280803B1 (en) | 2015-04-06 | 2021-05-26 | The Board of Trustees of the Leland Stanford Junior University | Chemically modified guide rnas for crispr/cas-mediated gene regulation |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and their uses |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| CN106198358B (zh) * | 2016-06-24 | 2019-02-15 | 北京大学 | 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法 |
| IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nuclear base editors and their uses |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| EP3592777A1 (en) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
| JP7191388B2 (ja) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| GB201708662D0 (en) | 2017-05-31 | 2017-07-12 | Tropic Biosciences Uk Ltd | Compositions and methods for increasing shelf-life of banana |
| WO2018220582A1 (en) | 2017-05-31 | 2018-12-06 | Tropic Biosciences UK Limited | Methods of selecting cells comprising genome editing events |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
| WO2020191243A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| GB201905542D0 (en) * | 2019-04-18 | 2019-06-05 | Phytoform Labs Ltd | Methods, systems and apparatus for plant material screening and propagation |
| US20230051935A1 (en) * | 2019-09-30 | 2023-02-16 | Calyxt, Inc. | Gene editing using a messenger ribonucleic acid construct |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| JP2024534945A (ja) | 2021-09-10 | 2024-09-26 | アジレント・テクノロジーズ・インク | 化学修飾を有するプライム編集のためのガイドｒｎａ |
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2013
- 2013-09-06 TW TW102132254A patent/TWI670004B/zh active
- 2013-09-06 US US14/020,694 patent/US20140075593A1/en not_active Abandoned
- 2013-09-06 AR ARP130103193A patent/AR092482A1/es unknown
- 2013-09-09 AU AU2013312198A patent/AU2013312198B2/en active Active
- 2013-09-09 CN CN201380054087.4A patent/CN104736712A/zh active Pending
- 2013-09-09 IN IN1785DEN2015 patent/IN2015DN01785A/en unknown
- 2013-09-09 EP EP13835630.8A patent/EP2893024B1/en active Active
- 2013-09-09 CA CA2883800A patent/CA2883800C/en active Active
- 2013-09-09 WO PCT/US2013/058766 patent/WO2014039970A1/en active Application Filing
- 2013-09-09 CN CN201910796679.2A patent/CN111118100A/zh active Pending
- 2013-09-09 KR KR1020157008557A patent/KR102219621B1/ko active IP Right Grant
- 2013-09-09 MX MX2015002964A patent/MX367170B/es active IP Right Grant
- 2013-09-09 RU RU2015112573A patent/RU2679510C2/ru active
- 2013-09-09 BR BR112015004864A patent/BR112015004864B8/pt active IP Right Grant
- 2013-09-09 JP JP2015531282A patent/JP6362600B2/ja active Active
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2015
- 2015-03-03 IL IL23753515A patent/IL237535B/en active IP Right Grant
- 2015-03-13 ZA ZA2015/01736A patent/ZA201501736B/en unknown
- 2015-11-09 HK HK15111003.5A patent/HK1210220A1/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20140075593A1 (en) | 2014-03-13 |
| JP2015527085A (ja) | 2015-09-17 |
| IN2015DN01785A (pt) | 2015-05-29 |
| AR092482A1 (es) | 2015-04-22 |
| CA2883800A1 (en) | 2014-03-13 |
| EP2893024B1 (en) | 2019-12-04 |
| RU2015112573A (ru) | 2016-10-27 |
| CN104736712A (zh) | 2015-06-24 |
| ZA201501736B (en) | 2016-10-26 |
| HK1210220A1 (en) | 2016-04-15 |
| AU2013312198A1 (en) | 2015-03-19 |
| EP2893024A4 (en) | 2016-03-30 |
| MX367170B (es) | 2019-08-07 |
| KR20150052241A (ko) | 2015-05-13 |
| JP6362600B2 (ja) | 2018-07-25 |
| TWI670004B (zh) | 2019-09-01 |
| IL237535B (en) | 2019-11-28 |
| TW201424578A (zh) | 2014-07-01 |
| RU2679510C2 (ru) | 2019-02-11 |
| WO2014039970A1 (en) | 2014-03-13 |
| EP2893024A1 (en) | 2015-07-15 |
| IL237535A0 (en) | 2015-04-30 |
| CN111118100A (zh) | 2020-05-08 |
| CA2883800C (en) | 2021-07-13 |
| BR112015004864B1 (pt) | 2022-10-25 |
| MX2015002964A (es) | 2015-06-05 |
| KR102219621B1 (ko) | 2021-02-24 |
| WO2014039970A9 (en) | 2014-10-09 |
| BR112015004864A2 (pt) | 2017-11-21 |
| AU2013312198B2 (en) | 2018-11-08 |
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