MX367170B - Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. - Google Patents

Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.

Info

Publication number
MX367170B
MX367170B MX2015002964A MX2015002964A MX367170B MX 367170 B MX367170 B MX 367170B MX 2015002964 A MX2015002964 A MX 2015002964A MX 2015002964 A MX2015002964 A MX 2015002964A MX 367170 B MX367170 B MX 367170B
Authority
MX
Mexico
Prior art keywords
etip
facs
enrichment
plant
cell sorting
Prior art date
Application number
MX2015002964A
Other languages
English (en)
Other versions
MX2015002964A (es
Inventor
Spangenberg German
Sahab Sareena
Mason John
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2015002964A publication Critical patent/MX2015002964A/es
Publication of MX367170B publication Critical patent/MX367170B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physiology (AREA)

Abstract

La presente invención se refiere a una plataforma de integración transgénica realizado por ingeniería (ETIP) que se puede insertar al azar, o en ubicaciones específicas en el genoma de las plantas para facilitar la rápida selección y detección de un GOl que está perfectamente dirigido (ambos extremos 3' y 5') en la localización ETIP genómica. Un elemento en la presente invención es la introducción de roturas de doble cadena específicas dentro de la ETIP. En algunas modalidades, se describe una ETIP que utiliza sitios de unión de nucleasa de dedo de zinc, pero puede utilizar otras tecnologías de segmentación tales como meganucleasas, TALS, CRISPR, o cremalleras de leucina. También se describen composiciones de, y métodos para producir, las plantas transgénicas en las que el donante o el ADN de carga útil expresa uno o más productos de una secuencia de ácido nucleico exógena (por ejemplo, proteína o ARN) que ha sido de manera estable integrada en una ETIP en una célula de la planta. En modalidades, la ETIP facilita la prueba de candidatos de genes y vectores de expresión de plantas para la ideación por medios de fases de desarrollo.
MX2015002964A 2012-09-07 2013-09-09 Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. MX367170B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261697890P 2012-09-07 2012-09-07
US14/020,694 US20140075593A1 (en) 2012-09-07 2013-09-06 Fluorescence activated cell sorting (facs) enrichment to generate plants
PCT/US2013/058766 WO2014039970A1 (en) 2012-09-07 2013-09-09 Fluorescence activated cell sorting (facs) enrichment to generate plants

Publications (2)

Publication Number Publication Date
MX2015002964A MX2015002964A (es) 2015-06-05
MX367170B true MX367170B (es) 2019-08-07

Family

ID=50234835

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015002964A MX367170B (es) 2012-09-07 2013-09-09 Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.

Country Status (17)

Country Link
US (1) US20140075593A1 (es)
EP (1) EP2893024B1 (es)
JP (1) JP6362600B2 (es)
KR (1) KR102219621B1 (es)
CN (2) CN111118100A (es)
AR (1) AR092482A1 (es)
AU (1) AU2013312198B2 (es)
BR (1) BR112015004864B8 (es)
CA (1) CA2883800C (es)
HK (1) HK1210220A1 (es)
IL (1) IL237535B (es)
IN (1) IN2015DN01785A (es)
MX (1) MX367170B (es)
RU (1) RU2679510C2 (es)
TW (1) TWI670004B (es)
WO (1) WO2014039970A1 (es)
ZA (1) ZA201501736B (es)

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Publication number Publication date
EP2893024A4 (en) 2016-03-30
HK1210220A1 (en) 2016-04-15
CA2883800A1 (en) 2014-03-13
JP6362600B2 (ja) 2018-07-25
EP2893024A1 (en) 2015-07-15
ZA201501736B (en) 2016-10-26
RU2015112573A (ru) 2016-10-27
CN104736712A (zh) 2015-06-24
WO2014039970A9 (en) 2014-10-09
BR112015004864A2 (pt) 2017-11-21
EP2893024B1 (en) 2019-12-04
JP2015527085A (ja) 2015-09-17
CN111118100A (zh) 2020-05-08
WO2014039970A1 (en) 2014-03-13
RU2679510C2 (ru) 2019-02-11
AR092482A1 (es) 2015-04-22
CA2883800C (en) 2021-07-13
AU2013312198A1 (en) 2015-03-19
TW201424578A (zh) 2014-07-01
KR102219621B1 (ko) 2021-02-24
TWI670004B (zh) 2019-09-01
IN2015DN01785A (es) 2015-05-29
US20140075593A1 (en) 2014-03-13
IL237535A0 (en) 2015-04-30
BR112015004864B8 (pt) 2023-05-16
IL237535B (en) 2019-11-28
MX2015002964A (es) 2015-06-05
KR20150052241A (ko) 2015-05-13
BR112015004864B1 (pt) 2022-10-25
AU2013312198B2 (en) 2018-11-08

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