MX367170B - Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. - Google Patents

Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.

Info

Publication number
MX367170B
MX367170B MX2015002964A MX2015002964A MX367170B MX 367170 B MX367170 B MX 367170B MX 2015002964 A MX2015002964 A MX 2015002964A MX 2015002964 A MX2015002964 A MX 2015002964A MX 367170 B MX367170 B MX 367170B
Authority
MX
Mexico
Prior art keywords
etip
facs
enrichment
plant
cell sorting
Prior art date
Application number
MX2015002964A
Other languages
English (en)
Other versions
MX2015002964A (es
Inventor
Spangenberg German
Sahab Sareena
Mason John
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2015002964A publication Critical patent/MX2015002964A/es
Publication of MX367170B publication Critical patent/MX367170B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Botany (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)

Abstract

La presente invención se refiere a una plataforma de integración transgénica realizado por ingeniería (ETIP) que se puede insertar al azar, o en ubicaciones específicas en el genoma de las plantas para facilitar la rápida selección y detección de un GOl que está perfectamente dirigido (ambos extremos 3' y 5') en la localización ETIP genómica. Un elemento en la presente invención es la introducción de roturas de doble cadena específicas dentro de la ETIP. En algunas modalidades, se describe una ETIP que utiliza sitios de unión de nucleasa de dedo de zinc, pero puede utilizar otras tecnologías de segmentación tales como meganucleasas, TALS, CRISPR, o cremalleras de leucina. También se describen composiciones de, y métodos para producir, las plantas transgénicas en las que el donante o el ADN de carga útil expresa uno o más productos de una secuencia de ácido nucleico exógena (por ejemplo, proteína o ARN) que ha sido de manera estable integrada en una ETIP en una célula de la planta. En modalidades, la ETIP facilita la prueba de candidatos de genes y vectores de expresión de plantas para la ideación por medios de fases de desarrollo.
MX2015002964A 2012-09-07 2013-09-09 Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. MX367170B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261697890P 2012-09-07 2012-09-07
US14/020,694 US20140075593A1 (en) 2012-09-07 2013-09-06 Fluorescence activated cell sorting (facs) enrichment to generate plants
PCT/US2013/058766 WO2014039970A1 (en) 2012-09-07 2013-09-09 Fluorescence activated cell sorting (facs) enrichment to generate plants

Publications (2)

Publication Number Publication Date
MX2015002964A MX2015002964A (es) 2015-06-05
MX367170B true MX367170B (es) 2019-08-07

Family

ID=50234835

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015002964A MX367170B (es) 2012-09-07 2013-09-09 Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.

Country Status (17)

Country Link
US (1) US20140075593A1 (es)
EP (1) EP2893024B1 (es)
JP (1) JP6362600B2 (es)
KR (1) KR102219621B1 (es)
CN (2) CN104736712A (es)
AR (1) AR092482A1 (es)
AU (1) AU2013312198B2 (es)
BR (1) BR112015004864B8 (es)
CA (1) CA2883800C (es)
HK (1) HK1210220A1 (es)
IL (1) IL237535B (es)
IN (1) IN2015DN01785A (es)
MX (1) MX367170B (es)
RU (1) RU2679510C2 (es)
TW (1) TWI670004B (es)
WO (1) WO2014039970A1 (es)
ZA (1) ZA201501736B (es)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9914930B2 (en) * 2012-09-07 2018-03-13 Dow Agrosciences Llc FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
CA2969619A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
KR102888521B1 (ko) 2015-04-06 2025-11-19 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106198358B (zh) * 2016-06-24 2019-02-15 北京大学 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11525119B2 (en) 2016-09-06 2022-12-13 The Children's Medical Center Corporation Immune cells derived from induced pluripotent stem cell
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
WO2018220582A1 (en) 2017-05-31 2018-12-06 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
GB201905542D0 (en) * 2019-04-18 2019-06-05 Phytoform Labs Ltd Methods, systems and apparatus for plant material screening and propagation
WO2021067394A1 (en) * 2019-09-30 2021-04-08 Calyxt, Inc. Gene editing using a messenger ribonucleic acid construct
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
JP2024534945A (ja) 2021-09-10 2024-09-26 アジレント・テクノロジーズ・インク 化学修飾を有するプライム編集のためのガイドｒｎａ
CN117701591B (zh) * 2023-12-25 2024-11-19 西北农林科技大学 与不结球白菜基因共分离的叶柄厚分子标记t33、引物及应用
CN120082500B (zh) * 2025-04-28 2026-01-27 北京艾普希隆生物科技有限公司 一种制备植物单细胞的方法

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4727028A (en) 1981-06-22 1988-02-23 Eli Lilly And Company Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
EP0320500B1 (en) 1983-01-13 2004-11-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Non-oncogenic ti plasmid vector system and recombinant DNA molecules for the introduction of expressible genes into plant cell genomes
EP0131624B1 (en) 1983-01-17 1992-09-16 Monsanto Company Plasmids for transforming plant cells
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
AU7360087A (en) 1986-04-30 1987-11-24 Boyce Thompson Institute For Plant Research Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
ES2074999T3 (es) 1987-05-20 1995-10-01 Ciba Geigy Ag Plantas de zea mays y plantas de zea mays transgenicas regeneradas de protoplastos o celulas derivadas de protoplastos.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5141131A (en) 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
ES2260886T3 (es) 1990-11-23 2006-11-01 Bayer Bioscience N.V. Procedimiento para transformar plantas monocotiledoneas.
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
JPH05130861A (ja) * 1991-11-06 1993-05-28 Hitachi Chem Co Ltd プロトプラストからクローン細胞を作製する方法
JPH07505531A (ja) 1992-04-15 1995-06-22 プラント・ジェネティック・システムズ・エヌ・ブイ 単子葉植物細胞の形質転換法
ATE398679T1 (de) 1992-07-07 2008-07-15 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
DE69404385T2 (de) 1993-06-04 1998-02-19 Cavis Srl Trägheitsschalter zur Ausschaltung der Batterie eines Kraftfahrzeuges
EP0861021B1 (en) 1995-10-13 2009-09-23 Dow Agrosciences LLC Modified bacillus thuringiensis gene for lepidopteran control in plants
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US20030219763A1 (en) * 1996-12-13 2003-11-27 Jen Sheen Plant protoplast gene expression systems and uses thereof
WO2000034468A2 (en) 1998-12-11 2000-06-15 Biomira Inc. Muc-1 antagonists and methods of treating immune disorders
ES2341217T3 (es) 1999-01-19 2010-06-17 Maxygen, Inc. Recombinacion de acidos nucleicos mediada por oligonucleotidos.
ATE538205T1 (de) 1999-11-29 2012-01-15 Midwest Oilseeds Inc Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen
JP3780333B2 (ja) * 2000-09-22 2006-05-31 国立大学法人大阪大学 外来性遺伝物質又は生理活性物質を細胞内へ導入する新規な方法
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
WO2003080809A2 (en) 2002-03-21 2003-10-02 Sangamo Biosciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
JP4022614B2 (ja) * 2002-03-25 2007-12-19 国立大学法人大阪大学 新規なバイオビーズの作製方法
HUE047016T2 (hu) 2004-03-26 2020-04-28 Dow Agrosciences Llc CRY1F és CRY1AC transzgenikus gyapotvonalak és eseményspecifikus azonosításuk
BRPI0716427A2 (pt) * 2006-08-11 2014-03-11 Dow Agrosciences Llc Recombinação homóloga mediada por nuclease de apêndice de zinco
ES2586210T3 (es) 2006-12-14 2016-10-13 Sangamo Biosciences, Inc. Proteínas de dedo de zinc no canónicas optimizadas
EP2188384B1 (en) 2007-09-27 2015-07-15 Sangamo BioSciences, Inc. Rapid in vivo identification of biologically active nucleases
EP2455454A1 (en) * 2010-11-19 2012-05-23 Albert-Ludwigs-Universität Freiburg Method for introducing a polynucleotide into plant protoplast cells
KR101933552B1 (ko) * 2011-06-07 2018-12-28 프라운호퍼 게젤샤프트 쭈르 푀르데룽 데어 안겐반텐 포르슝 에. 베. 단클론 식물세포주의 발생 방법

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Publication number Publication date
WO2014039970A1 (en) 2014-03-13
KR102219621B1 (ko) 2021-02-24
RU2679510C2 (ru) 2019-02-11
EP2893024A4 (en) 2016-03-30
JP6362600B2 (ja) 2018-07-25
CA2883800C (en) 2021-07-13
TWI670004B (zh) 2019-09-01
CN111118100A (zh) 2020-05-08
CN104736712A (zh) 2015-06-24
IL237535A0 (en) 2015-04-30
RU2015112573A (ru) 2016-10-27
BR112015004864A2 (pt) 2017-11-21
IL237535B (en) 2019-11-28
ZA201501736B (en) 2016-10-26
KR20150052241A (ko) 2015-05-13
EP2893024B1 (en) 2019-12-04
BR112015004864B8 (pt) 2023-05-16
CA2883800A1 (en) 2014-03-13
TW201424578A (zh) 2014-07-01
WO2014039970A9 (en) 2014-10-09
US20140075593A1 (en) 2014-03-13
AR092482A1 (es) 2015-04-22
EP2893024A1 (en) 2015-07-15
AU2013312198A1 (en) 2015-03-19
AU2013312198B2 (en) 2018-11-08
HK1210220A1 (en) 2016-04-15
BR112015004864B1 (pt) 2022-10-25
JP2015527085A (ja) 2015-09-17
MX2015002964A (es) 2015-06-05
IN2015DN01785A (es) 2015-05-29

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