MX367170B - Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. - Google Patents

Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.

Info

Publication number
MX367170B
MX367170B MX2015002964A MX2015002964A MX367170B MX 367170 B MX367170 B MX 367170B MX 2015002964 A MX2015002964 A MX 2015002964A MX 2015002964 A MX2015002964 A MX 2015002964A MX 367170 B MX367170 B MX 367170B
Authority
MX
Mexico
Prior art keywords
etip
facs
enrichment
plant
cell sorting
Prior art date
Application number
MX2015002964A
Other languages
English (en)
Other versions
MX2015002964A (es
Inventor
Spangenberg German
Sahab Sareena
Mason John
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2015002964A publication Critical patent/MX2015002964A/es
Publication of MX367170B publication Critical patent/MX367170B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)

Abstract

La presente invención se refiere a una plataforma de integración transgénica realizado por ingeniería (ETIP) que se puede insertar al azar, o en ubicaciones específicas en el genoma de las plantas para facilitar la rápida selección y detección de un GOl que está perfectamente dirigido (ambos extremos 3' y 5') en la localización ETIP genómica. Un elemento en la presente invención es la introducción de roturas de doble cadena específicas dentro de la ETIP. En algunas modalidades, se describe una ETIP que utiliza sitios de unión de nucleasa de dedo de zinc, pero puede utilizar otras tecnologías de segmentación tales como meganucleasas, TALS, CRISPR, o cremalleras de leucina. También se describen composiciones de, y métodos para producir, las plantas transgénicas en las que el donante o el ADN de carga útil expresa uno o más productos de una secuencia de ácido nucleico exógena (por ejemplo, proteína o ARN) que ha sido de manera estable integrada en una ETIP en una célula de la planta. En modalidades, la ETIP facilita la prueba de candidatos de genes y vectores de expresión de plantas para la ideación por medios de fases de desarrollo.
MX2015002964A 2012-09-07 2013-09-09 Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. MX367170B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261697890P 2012-09-07 2012-09-07
US14/020,694 US20140075593A1 (en) 2012-09-07 2013-09-06 Fluorescence activated cell sorting (facs) enrichment to generate plants
PCT/US2013/058766 WO2014039970A1 (en) 2012-09-07 2013-09-09 Fluorescence activated cell sorting (facs) enrichment to generate plants

Publications (2)

Publication Number Publication Date
MX2015002964A MX2015002964A (es) 2015-06-05
MX367170B true MX367170B (es) 2019-08-07

Family

ID=50234835

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015002964A MX367170B (es) 2012-09-07 2013-09-09 Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.

Country Status (17)

Country Link
US (1) US20140075593A1 (es)
EP (1) EP2893024B1 (es)
JP (1) JP6362600B2 (es)
KR (1) KR102219621B1 (es)
CN (2) CN104736712A (es)
AR (1) AR092482A1 (es)
AU (1) AU2013312198B2 (es)
BR (1) BR112015004864B8 (es)
CA (1) CA2883800C (es)
HK (1) HK1210220A1 (es)
IL (1) IL237535B (es)
IN (1) IN2015DN01785A (es)
MX (1) MX367170B (es)
RU (1) RU2679510C2 (es)
TW (1) TWI670004B (es)
WO (1) WO2014039970A1 (es)
ZA (1) ZA201501736B (es)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
AU2013312538B2 (en) * 2012-09-07 2019-01-24 Corteva Agriscience Llc FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
AU2015355546B2 (en) 2014-12-03 2021-10-14 Agilent Technologies, Inc. Guide RNA with chemical modifications
WO2016164356A1 (en) 2015-04-06 2016-10-13 The Board Of Trustees Of The Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CN106198358B (zh) * 2016-06-24 2019-02-15 北京大学 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法
GB2568182A (en) 2016-08-03 2019-05-08 Harvard College Adenosine nucleobase editors and uses thereof
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
US11268082B2 (en) 2017-03-23 2022-03-08 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
US20200109408A1 (en) 2017-05-31 2020-04-09 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019058255A1 (en) 2017-09-19 2019-03-28 Tropic Biosciences UK Limited MODIFICATION OF THE SPECIFICITY OF VEGETABLE NON-CODANT RNA MOLECULES FOR THE SILENCING OF GENE EXPRESSION
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
AU2020240109A1 (en) 2019-03-19 2021-09-30 President And Fellows Of Harvard College Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
GB201905542D0 (en) * 2019-04-18 2019-06-05 Phytoform Labs Ltd Methods, systems and apparatus for plant material screening and propagation
EP4022055A4 (en) * 2019-09-30 2024-01-03 Calyxt, Inc. GENE EDITING USING A MESSENGER RIBONUCLEIC ACID CONSTRUCTION
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
WO2023039586A1 (en) 2021-09-10 2023-03-16 Agilent Technologies, Inc. Guide rnas with chemical modification for prime editing
CN117701591B (zh) * 2023-12-25 2024-11-19 西北农林科技大学 与不结球白菜基因共分离的叶柄厚分子标记t33、引物及应用
CN120082500A (zh) * 2025-04-28 2025-06-03 北京艾普希隆生物科技有限公司 一种制备植物单细胞的方法

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4727028A (en) 1981-06-22 1988-02-23 Eli Lilly And Company Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
ATE52800T1 (de) 1983-01-13 1990-06-15 Max Planck Gesellschaft Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren.
WO1984002919A1 (en) 1983-01-17 1984-08-02 Monsanto Co Plasmids for transforming plant cells
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
EP0265502A1 (en) 1986-04-30 1988-05-04 Boyce Thompson Institute For Plant Research, Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
EP0846771A1 (en) 1987-05-20 1998-06-10 Novartis AG Zea mays plants and transgenic zea mays plants regenerated from protoplasts or protoplast-derived cells
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5141131A (en) 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
DE69133512T2 (de) 1990-11-23 2006-09-28 Bayer Bioscience N.V. Verfahren zur Transformation monokotyler Pflanzen
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
JPH05130861A (ja) * 1991-11-06 1993-05-28 Hitachi Chem Co Ltd プロトプラストからクローン細胞を作製する方法
WO1993021335A2 (en) 1992-04-15 1993-10-28 Plant Genetic Systems, N.V. Transformation of monocot cells
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
ES2105415T3 (es) 1993-06-04 1997-10-16 Cavis Srl Dispositivo de inercia para interrumpir el circuito electrico de un vehiculo con un motor de combustion interna.
CA2234656C (en) 1995-10-13 2008-01-08 Dow Agrosciences Llc Modified bacillus thuringiensis gene for lepidopteran control in plants
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US20030219763A1 (en) * 1996-12-13 2003-11-27 Jen Sheen Plant protoplast gene expression systems and uses thereof
CA2354644A1 (en) 1998-12-11 2000-06-15 Biomira, Inc. Muc-1 antagonists and methods of treating immune disorders
WO2000042560A2 (en) 1999-01-19 2000-07-20 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides
US6809232B1 (en) 1999-11-29 2004-10-26 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
JP3780333B2 (ja) * 2000-09-22 2006-05-31 国立大学法人大阪大学 外来性遺伝物質又は生理活性物質を細胞内へ導入する新規な方法
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
ATE531796T1 (de) 2002-03-21 2011-11-15 Sangamo Biosciences Inc Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination
JP4022614B2 (ja) * 2002-03-25 2007-12-19 国立大学法人大阪大学 新規なバイオビーズの作製方法
ES2743789T3 (es) 2004-03-26 2020-02-20 Dow Agrosciences Llc Líneas de algodón transgénico Cry1F y Cry1Ac y su identificación específica de evento
JP2010500029A (ja) * 2006-08-11 2010-01-07 ダウ アグロサイエンシィズ エルエルシー ジンクフィンガーヌクレアーゼ媒介相同組換え
JP5632610B2 (ja) 2006-12-14 2014-11-26 ダウ アグロサイエンシィズ エルエルシー 最適化された非正準ジンクフィンガータンパク質
EP2188384B1 (en) 2007-09-27 2015-07-15 Sangamo BioSciences, Inc. Rapid in vivo identification of biologically active nucleases
EP2455454A1 (en) * 2010-11-19 2012-05-23 Albert-Ludwigs-Universität Freiburg Method for introducing a polynucleotide into plant protoplast cells
US10480001B2 (en) * 2011-06-07 2019-11-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for the generation of a monoclonal plant cell line

Also Published As

Publication number Publication date
JP2015527085A (ja) 2015-09-17
RU2015112573A (ru) 2016-10-27
TWI670004B (zh) 2019-09-01
CA2883800A1 (en) 2014-03-13
BR112015004864A2 (pt) 2017-11-21
BR112015004864B8 (pt) 2023-05-16
HK1210220A1 (en) 2016-04-15
EP2893024A1 (en) 2015-07-15
TW201424578A (zh) 2014-07-01
EP2893024B1 (en) 2019-12-04
KR20150052241A (ko) 2015-05-13
US20140075593A1 (en) 2014-03-13
IL237535B (en) 2019-11-28
WO2014039970A1 (en) 2014-03-13
AR092482A1 (es) 2015-04-22
WO2014039970A9 (en) 2014-10-09
CA2883800C (en) 2021-07-13
IN2015DN01785A (es) 2015-05-29
RU2679510C2 (ru) 2019-02-11
CN104736712A (zh) 2015-06-24
EP2893024A4 (en) 2016-03-30
IL237535A0 (en) 2015-04-30
JP6362600B2 (ja) 2018-07-25
BR112015004864B1 (pt) 2022-10-25
KR102219621B1 (ko) 2021-02-24
ZA201501736B (en) 2016-10-26
CN111118100A (zh) 2020-05-08
AU2013312198A1 (en) 2015-03-19
MX2015002964A (es) 2015-06-05
AU2013312198B2 (en) 2018-11-08

Similar Documents

Publication Publication Date Title
MX367170B (es) Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.
NZ705675A (en) Engineered transgene integration platform (etip) for gene targeting and trait stacking
EP4372084A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2014172489A3 (en) Targeted modification of rat genome
WO2015160895A3 (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
MX367666B (es) Composiciones y métodos para cuantificar una secuencia de ácido nucleico en una muestra.
NZ705745A (en) Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
NZ705742A (en) Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
EA201890772A1 (ru) Способ высокоуровневого и стабильного переноса генов в лимфоцитах
EP4368715A3 (en) Nucleic acid sequence analysis from single cells
GB2487341A (en) Compositions and methods for targeted nucleic acid sequence selection and amplification
WO2016033251A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
GB2518764A8 (en) Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
MX2016003638A (es) Anticuerpos con cadena ligera modificada con histidina por ingenieria genetica y animales no humanos geneticamente modificados para generarlos.
WO2012021493A3 (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
SG11201809242VA (en) Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor
WO2012017329A8 (en) Method for targeted genomic events in algae
ATE490319T1 (de) Auswahl und anreicherung von proteinen durch in- vitro-untergliederung
WO2018231955A3 (en) Isolation of target nucleic acids
WO2014152091A3 (en) Methods of genome sequencing and epigenetic analysis
WO2010039189A3 (en) Methods for sequencing degraded or modified nucleic acids
WO2014146181A3 (pt) Promotor específico de folhas, gene quimérico, vetor de recombinante, planta ou parte de planta transformada e métodos contendo promotor específico de folhas para modificar a expressão de genes de interesse em plantas
WO2014122422A3 (en) Amplification technique
MX2015007370A (es) Recuperacion del adn genomico de las muestras de semillas extraidas remanentes.
GB201014818D0 (en) Gene regulation

Legal Events

Date Code Title Description
FG Grant or registration