WO2013082164A1 - Enhanced ligation reactions - Google Patents

Enhanced ligation reactions Download PDF

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Publication number
WO2013082164A1
WO2013082164A1 PCT/US2012/066869 US2012066869W WO2013082164A1 WO 2013082164 A1 WO2013082164 A1 WO 2013082164A1 US 2012066869 W US2012066869 W US 2012066869W WO 2013082164 A1 WO2013082164 A1 WO 2013082164A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
embodiments
ligase
enzyme
method
Prior art date
Application number
PCT/US2012/066869
Other languages
French (fr)
Inventor
Stephen Hendricks
David King
Lei Xi
Marian Peris
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201161564243P priority Critical
Priority to US61/564,243 priority
Priority to US13/328,844 priority
Priority to USPCT/US2011/065535 priority
Priority to US13/328,844 priority patent/US20120156728A1/en
Priority to PCT/US2011/065535 priority patent/WO2012083189A2/en
Priority to PCT/US2012/021465 priority patent/WO2012099832A2/en
Priority to USPCT/US2012/021465 priority
Priority to US61/692,830 priority
Priority to US201261692830P priority
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Priority claimed from US14/360,892 external-priority patent/US9315861B2/en
Publication of WO2013082164A1 publication Critical patent/WO2013082164A1/en

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