DE602005020421D1 - Verfahren zur analyse von polynukleotidsequenzen - Google Patents

Verfahren zur analyse von polynukleotidsequenzen

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Publication number
DE602005020421D1
DE602005020421D1 DE602005020421T DE602005020421T DE602005020421D1 DE 602005020421 D1 DE602005020421 D1 DE 602005020421D1 DE 602005020421 T DE602005020421 T DE 602005020421T DE 602005020421 T DE602005020421 T DE 602005020421T DE 602005020421 D1 DE602005020421 D1 DE 602005020421D1
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Prior art keywords
methods
polynucleotides
facilitate
analysis
kits
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DE602005020421T
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English (en)
Inventor
Stephen Quake
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Helicos BioSciences Corp
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Helicos BioSciences Corp
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Publication of DE602005020421D1 publication Critical patent/DE602005020421D1/de
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
DE602005020421T 2004-02-19 2005-02-09 Verfahren zur analyse von polynukleotidsequenzen Active DE602005020421D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US54627704P 2004-02-19 2004-02-19
US54761104P 2004-02-24 2004-02-24
PCT/US2005/004258 WO2005080605A2 (en) 2004-02-19 2005-02-09 Methods and kits for analyzing polynucleotide sequences

Publications (1)

Publication Number Publication Date
DE602005020421D1 true DE602005020421D1 (de) 2010-05-20

Family

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Family Applications (1)

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DE602005020421T Active DE602005020421D1 (de) 2004-02-19 2005-02-09 Verfahren zur analyse von polynukleotidsequenzen

Country Status (6)

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US (2) US7981604B2 (de)
EP (2) EP2248911A1 (de)
AT (1) ATE463584T1 (de)
CA (1) CA2557177A1 (de)
DE (1) DE602005020421D1 (de)
WO (1) WO2005080605A2 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69825601T2 (de) * 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7211414B2 (en) * 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
CN1791682B (zh) 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7170050B2 (en) * 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
CA2579150C (en) * 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US20080264863A1 (en) * 2004-12-03 2008-10-30 California Institute Of Technology Microfluidic Sieve Valves
WO2006084132A2 (en) 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
EP2463386B1 (de) 2005-06-15 2017-04-12 Complete Genomics Inc. Nukleinsäureanalyse durch zufällige Mischungen von sich überlappenden Fragmenten
CA2616433A1 (en) * 2005-07-28 2007-02-01 Helicos Biosciences Corporation Consecutive base single molecule sequencing
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1940755A2 (de) 2005-10-28 2008-07-09 Praecis Pharmaceuticals Inc. Verfahren zur identifikation von verbindungen von interesse mithilfe kodierter bibliotheken
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
US20090075252A1 (en) * 2006-04-14 2009-03-19 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20090062129A1 (en) * 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
US20080081330A1 (en) * 2006-09-28 2008-04-03 Helicos Biosciences Corporation Method and devices for analyzing small RNA molecules
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US8612161B2 (en) * 2008-03-19 2013-12-17 Intelligent Biosystems Inc. Methods and compositions for base calling nucleic acids
US8703422B2 (en) * 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CA2689626C (en) * 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CA2693979A1 (en) * 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
EP2198293B1 (de) * 2007-09-07 2012-01-18 Fluidigm Corporation Verfahren und system zur bestimmung von genkopiezahlvarianten
US20090191553A1 (en) * 2007-10-01 2009-07-30 Applied Biosystems Inc. Chase Ligation Sequencing
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009073629A2 (en) 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN104862383B (zh) 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US20100056382A1 (en) * 2008-05-30 2010-03-04 Sussman Michael R Dna millichip
JP5268444B2 (ja) * 2008-06-23 2013-08-21 株式会社日立ハイテクノロジーズ 単分子リアルタイムシーケンス装置,核酸分析装置及び単分子リアルタイムシーケンス方法
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US8993230B2 (en) * 2008-12-04 2015-03-31 Pacific Biosciences of Californ, Inc. Asynchronous sequencing of biological polymers
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
EP2248915B1 (de) * 2009-05-05 2013-09-18 Qiagen GmbH Detektion mehrerer Nukleinsäuresequenzen in einer Reaktionskartusche
CN102449527B (zh) * 2009-06-02 2015-08-19 株式会社尼康 图像处理装置、图像处理方法和显微镜
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US9416409B2 (en) * 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
US9339813B2 (en) * 2009-12-18 2016-05-17 Koninklijke Philips N.V. Substance determining apparatus
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
WO2011127933A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
US9670243B2 (en) * 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8834847B2 (en) 2010-08-12 2014-09-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
WO2013096692A1 (en) 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
DE102012102983A1 (de) 2012-04-05 2013-10-10 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen eines kritischen Winkels eines Anregungslichtstrahls
EP2891709A4 (de) * 2012-08-30 2016-04-13 Hitachi High Tech Corp Nukleinsäureanalysevorrichtung
US20140287946A1 (en) * 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
CN105378113B (zh) 2013-05-06 2020-02-21 加利福尼亚太平洋生物科学股份有限公司 实时电子测序
CN110452809B (zh) 2013-11-17 2022-07-01 宽腾矽公司 与测定芯片通过接口连接的仪器
US20170218416A1 (en) * 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
US11098303B2 (en) 2014-07-30 2021-08-24 President And Fellows Of Harvard College Systems and methods for determining nucleic acids
MX2017001807A (es) 2014-08-08 2018-02-08 Quantum Si Inc Sistema optico y circuito integrado de valoración para sondear, detectar y analizar moleculas.
KR20220165286A (ko) 2014-08-08 2022-12-14 퀀텀-에스아이 인코포레이티드 분자를 프로빙, 검출 및 분석하기 위한 외부 광원을 갖는 통합 디바이스
EP3471402B1 (de) 2014-08-08 2023-05-31 Quantum-Si Incorporated Integrierte vorrichtung zur temporären gruppierung von empfangenen photonen
US11466316B2 (en) 2015-05-20 2022-10-11 Quantum-Si Incorporated Pulsed laser and bioanalytic system
US10246742B2 (en) 2015-05-20 2019-04-02 Quantum-Si Incorporated Pulsed laser and bioanalytic system
US10605730B2 (en) 2015-05-20 2020-03-31 Quantum-Si Incorporated Optical sources for fluorescent lifetime analysis
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
WO2017024049A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Single-molecule nanofet sequencing systems and methods
CN105112516A (zh) * 2015-08-14 2015-12-02 深圳市瀚海基因生物科技有限公司 一种单分子靶向测序方法、装置、系统及应用
EP3368692B1 (de) * 2015-10-29 2021-07-21 Temple University Of The Commonwealth System Of Higher Education Modifizierung von 3'-endstücken von nukleinsäuren durch dna-polymerase-theta
JP2019512090A (ja) 2016-02-17 2019-05-09 テッセラクト ヘルス インコーポレイテッドTesseract Health,Inc. 寿命イメージングおよび検出用途のためのセンサおよびデバイス
JP7064665B2 (ja) 2016-03-07 2022-05-11 ファーザー フラナガンズ ボーイズ ホーム ドゥーイング ビジネス アズ ボーイズ タウン ナショナル リサーチ ホスピタル 非侵襲的分子対照
WO2018112444A1 (en) 2016-12-16 2018-06-21 Quantum-Si Incorporated Compact mode-locked laser module
JP7050068B2 (ja) 2016-12-16 2022-04-07 クアンタム-エスアイ インコーポレイテッド コンパクトなビーム成形およびステアリングアセンブリ
CN110325651B (zh) * 2016-12-22 2024-03-15 伊鲁米那股份有限公司 具有质量控制示踪剂的阵列
EP4224530A1 (de) 2016-12-22 2023-08-09 Quantum-si Incorporated Integrierter photodetektor mit direktem pixel-binning
EP3574109B1 (de) 2017-01-24 2023-07-26 Vastogen, Inc. Verfahren zur konstruktion von kopien von nukleinsäuremolekülen
DK3610267T3 (da) * 2017-04-13 2023-02-27 European Molecular Biology Laboratory Massespektrometri til enkeltcellebilleddannelse
EP3625240A4 (de) * 2017-05-18 2021-01-27 Ultima Genomics, Inc. Sequenzierung mit nichtnatürlichen nukleotiden
WO2018218150A1 (en) 2017-05-26 2018-11-29 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
CA3100987A1 (en) 2018-06-15 2019-12-19 Quantum-Si Incorporated Data acquisition control for advanced analytic instruments having pulsed optical sources
KR20210022688A (ko) 2018-06-22 2021-03-03 퀀텀-에스아이 인코포레이티드 가변 검출 시간의 전하 저장 빈을 갖는 집적 광검출기
JP2022537277A (ja) 2019-06-14 2022-08-25 クアンタム-エスアイ インコーポレイテッド 改善されたビームアラインメント感度を有するスライス格子カプラ
CN114152599B (zh) * 2021-12-01 2023-10-13 中国农业大学 一种基于双劈裂功能核酸变构的孔雀石绿生物传感器

Family Cites Families (476)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
JPS5941169B2 (ja) 1975-12-25 1984-10-05 シチズン時計株式会社 エラストマ−ヒヨウジソウチ
US4153855A (en) 1977-12-16 1979-05-08 The United States Of America As Represented By The Secretary Of The Army Method of making a plate having a pattern of microchannels
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4344064A (en) 1979-12-06 1982-08-10 Western Electric Co., Inc. Article carrying a distinctive mark
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5260433A (en) 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US4707237A (en) 1982-09-09 1987-11-17 Ciba Corning Diagnostics Corp. System for identification of cells by electrophoresis
US5198540A (en) 1982-10-28 1993-03-30 Hubert Koster Process for the preparation of oligonucleotides in solution
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
USRE34069E (en) 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US4581624A (en) 1984-03-01 1986-04-08 Allied Corporation Microminiature semiconductor valve
US5360523A (en) 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5258506A (en) 1984-10-16 1993-11-02 Chiron Corporation Photolabile reagents for incorporation into oligonucleotide chains
US6060237A (en) 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4865968A (en) 1985-04-01 1989-09-12 The Salk Institute For Biological Studies DNA sequencing
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US5242797A (en) 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4994372A (en) 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5001060A (en) * 1987-02-06 1991-03-19 Lubrizol Enterprises, Inc. Plant anaerobic regulatory element
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US4994368A (en) 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
CH679555A5 (de) 1989-04-11 1992-03-13 Westonbridge Int Ltd
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US6379895B1 (en) 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5112736A (en) 1989-06-14 1992-05-12 University Of Utah Dna sequencing using fluorescence background electroblotting membrane
JPH04501449A (ja) 1989-06-14 1992-03-12 ウエストンブリッジ インターナショナル リミティド マイクロポンプ
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5096554A (en) 1989-08-07 1992-03-17 Applied Biosystems, Inc. Nucleic acid fractionation by counter-migration capillary electrophoresis
FR2650840B1 (fr) 1989-08-11 1991-11-29 Bertin & Cie Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
AU649407B2 (en) * 1989-09-21 1994-05-26 Infigen, Inc. Enhanced gene expression in response to lactation signals
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
CA2028261C (en) 1989-10-28 1995-01-17 Won Suck Yang Non-invasive method and apparatus for measuring blood glucose concentration
KR910012538A (ko) 1989-12-27 1991-08-08 야마무라 가쯔미 마이크로 펌프 및 그 제조 방법
US5091652A (en) 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
WO1991011533A1 (en) 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
DE4006152A1 (de) 1990-02-27 1991-08-29 Fraunhofer Ges Forschung Mikrominiaturisierte pumpe
US5096388A (en) 1990-03-22 1992-03-17 The Charles Stark Draper Laboratory, Inc. Microfabricated pump
SE470347B (sv) 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system
US5259737A (en) 1990-07-02 1993-11-09 Seiko Epson Corporation Micropump with valve structure
ES2155822T3 (es) 1990-12-06 2001-06-01 Affymetrix Inc Compuestos y su utilizacion en una estrategia de sintesis binaria.
EP0834576B1 (de) 1990-12-06 2002-01-16 Affymetrix, Inc. (a Delaware Corporation) Detektion von Nukleinsäuresequenzen
US5529679A (en) 1992-02-28 1996-06-25 Hitachi, Ltd. DNA detector and DNA detection method
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5762876A (en) 1991-03-05 1998-06-09 Molecular Tool, Inc. Automatic genotype determination
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5167784A (en) 1991-09-04 1992-12-01 Xerox Corporation Sequencing large nucleic acid fragments
WO1993005183A1 (en) 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
DE4135655A1 (de) 1991-09-11 1993-03-18 Fraunhofer Ges Forschung Mikrominiaturisierte, elektrostatisch betriebene membranpumpe
US5265327A (en) 1991-09-13 1993-11-30 Faris Sadeg M Microchannel plate technology
JP2001524926A (ja) 1991-09-18 2001-12-04 アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ オリゴマーの雑多ライブラリーの集合体を合成する方法
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5756285A (en) 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
DE69233391D1 (de) 1991-11-07 2004-09-02 Nanotronics Inc Hybridisierung von mit Chromophoren und Fluorophoren konjugierten Polynukleotiden zur Erzeugung eines Donor-Donor Energietransfersystems
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
WO1993013225A1 (en) 1991-12-23 1993-07-08 Chiron Corporation Htlv-1 probes for use in solution phase sandwich hybridization assays
US6555349B1 (en) 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
US5209834A (en) 1992-03-09 1993-05-11 The United States Of America As Represented By The United States Department Of Energy Ordered transport and identification of particles
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
DE4220077A1 (de) 1992-06-19 1993-12-23 Bosch Gmbh Robert Mikropumpe
JPH0622798A (ja) 1992-07-07 1994-02-01 Hitachi Ltd 塩基配列決定法
US5284568A (en) 1992-07-17 1994-02-08 E. I. Du Pont De Nemours And Company Disposable cartridge for ion selective electrode sensors
US5306403A (en) 1992-08-24 1994-04-26 Martin Marietta Energy Systems, Inc. Raman-based system for DNA sequencing-mapping and other separations
US5403709A (en) 1992-10-06 1995-04-04 Hybridon, Inc. Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
AU671820B2 (en) 1993-02-10 1996-09-12 Promega Corporation Non-radioactive DNA sequencing
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
WO1995000530A1 (en) 1993-06-25 1995-01-05 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5547859A (en) 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
DE4326466A1 (de) 1993-08-06 1995-02-09 Boehringer Mannheim Gmbh Infrarot-Farbstoff-markierte Nucleotide und ihre Verwendung in der Nucleinsäure-Detektion
US6376178B1 (en) 1993-09-03 2002-04-23 Duke University Method of nucleic acid sequencing
US5659171A (en) 1993-09-22 1997-08-19 Northrop Grumman Corporation Micro-miniature diaphragm pump for the low pressure pumping of gases
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US5470710A (en) 1993-10-22 1995-11-28 University Of Utah Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US6309601B1 (en) 1993-11-01 2001-10-30 Nanogen, Inc. Scanning optical detection system
AU703472B2 (en) 1993-11-02 1999-03-25 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
CH689836A5 (fr) 1994-01-14 1999-12-15 Westonbridge Int Ltd Micropompe.
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
ATE247716T1 (de) 1994-02-07 2003-09-15 Beckman Coulter Inc Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
JP3448090B2 (ja) 1994-02-16 2003-09-16 浜松ホトニクス株式会社 エネルギー移動検出法およびその装置
DE69518743T2 (de) 1994-03-08 2000-12-28 Amersham Pharm Biotech Uk Ltd Modifizierung von nukleotidanalogen
US20020168642A1 (en) 1994-06-06 2002-11-14 Andrzej Drukier Sequencing duplex DNA by mass spectroscopy
US6287850B1 (en) 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
US6379897B1 (en) 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US5834189A (en) 1994-07-08 1998-11-10 Visible Genetics Inc. Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US5853979A (en) 1995-06-30 1998-12-29 Visible Genetics Inc. Method and system for DNA sequence determination and mutation detection with reference to a standard
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US5872244A (en) 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
DE4433894A1 (de) 1994-09-22 1996-03-28 Fraunhofer Ges Forschung Verfahren und Vorrichtung zur Ansteuerung einer Mikropumpe
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
EP0706004B1 (de) 1994-10-07 2003-08-06 Bayer Corporation Entlastungsventil
US6654505B2 (en) 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5707506A (en) 1994-10-28 1998-01-13 Battelle Memorial Institute Channel plate for DNA sequencing
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5514256A (en) 1994-10-28 1996-05-07 Battelle Memorial Institute Apparatus for improved DNA sequencing
CA2204912C (en) 1994-11-10 2005-01-04 David Sarnoff Research Center, Inc. Liquid distribution system
US5603351A (en) 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US5556790A (en) 1994-12-05 1996-09-17 Pettit; John W. Method for Automated DNA sequencing
US5710628A (en) 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
US5786142A (en) 1995-05-30 1998-07-28 Visible Genetics Inc. Electrophoresis and fluorescence detection method
SE9500342D0 (sv) 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
SE9500589L (sv) 1995-02-17 1996-08-18 Rudolf Rigler Nukleinsyremolekyl detektion på fasta substrat vid enmolekyl DNA sekvensering
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5876187A (en) 1995-03-09 1999-03-02 University Of Washington Micropumps with fixed valves
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5994058A (en) 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5675155A (en) 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5885813A (en) 1995-05-31 1999-03-23 Amersham Life Science, Inc. Thermostable DNA polymerases
US6077664A (en) 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5861287A (en) 1995-06-23 1999-01-19 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
WO1997001440A1 (en) 1995-06-26 1997-01-16 Minnesota Mining And Manufacturing Company Multilayer polymer film with additional coatings or layers
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5916747A (en) 1995-06-30 1999-06-29 Visible Genetics Inc. Method and apparatus for alignment of signals for use in DNA based-calling
US5981186A (en) 1995-06-30 1999-11-09 Visible Genetics, Inc. Method and apparatus for DNA-sequencing using reduced number of sequencing mixtures
US5807679A (en) 1995-07-07 1998-09-15 Myriad Genetics, Inc. Island hopping--a method to sequence rapidly very large fragments of DNA
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
DE19527155C2 (de) 1995-07-25 1998-12-10 Deutsches Krebsforsch Verfahren zur Sequenzierung durch Oligomerhybridisierung
US5948614A (en) 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US5733729A (en) 1995-09-14 1998-03-31 Affymetrix, Inc. Computer-aided probability base calling for arrays of nucleic acid probes on chips
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5843655A (en) 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US6132580A (en) 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US6165765A (en) 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
SE9504099D0 (sv) 1995-11-16 1995-11-16 Pharmacia Biotech Ab A method of sequencing
US5776767A (en) 1995-12-12 1998-07-07 Visible Genetics Inc. Virtual DNA sequencer
US5705018A (en) 1995-12-13 1998-01-06 Hartley; Frank T. Micromachined peristaltic pump
US6147205A (en) 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
DE69613856T2 (de) 1995-12-15 2002-04-04 Amersham Pharm Biotech Inc Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
US5945283A (en) 1995-12-18 1999-08-31 Washington University Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer
KR100207410B1 (ko) 1995-12-19 1999-07-15 전주범 광로 조절 장치의 제조방법
US5789168A (en) 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5795722A (en) 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5830657A (en) 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US7468243B2 (en) 2001-03-12 2008-12-23 Affymetrix, Inc. 2-aminopyrimidin-4-one nucleic acid labeling compounds
US20010018514A1 (en) 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
TW406461B (en) 1996-03-01 2000-09-21 Cooper Ind Inc Enhanced polymer ic weathershed and surge arrester and method of making same
AU2217597A (en) 1996-03-18 1997-10-22 Sequenom, Inc. Dna sequencing by mass spectrometry
AU2320597A (en) 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US6361937B1 (en) 1996-03-19 2002-03-26 Affymetrix, Incorporated Computer-aided nucleic acid sequencing
SE9601318D0 (sv) 1996-04-04 1996-04-04 Pharmacia Biosensor Ab Method for nucleic acid analysis
WO1997039150A1 (en) 1996-04-15 1997-10-23 University Of Southern California Synthesis of fluorophore-labeled dna
US5867266A (en) 1996-04-17 1999-02-02 Cornell Research Foundation, Inc. Multiple optical channels for chemical analysis
US6432634B1 (en) 1996-04-18 2002-08-13 Visible Genetics Inc. Method, apparatus and kits for sequencing of nucleic acids using multiple dyes
EP0898596B1 (de) 1996-04-19 2001-08-29 Amersham Pharmacia Biotech UK Limited Quadratfarbstoffe und deren verwendung in einem fluorescenzsequenzierungsverfahren
JP3418292B2 (ja) 1996-04-24 2003-06-16 株式会社日立製作所 遺伝子解析装置
EP0896632B1 (de) 1996-05-01 2001-12-12 Visible Genetics Inc. Verfahren zum nachweis und zur identifizierung von mikroorganismen
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5981956A (en) 1996-05-16 1999-11-09 Affymetrix, Inc. Systems and methods for detection of labeled materials
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
JP2000512498A (ja) 1996-06-14 2000-09-26 サルノフ コーポレーション ポリヌクレオチドのシークエンス法
US5771124A (en) 1996-07-02 1998-06-23 Siliscape Compact display system with two stage magnification and immersed beam splitter
CA2234736A1 (en) 1996-07-16 1998-01-22 Periannan Senapathy Method for contiguous genome sequencing
WO1998003684A1 (en) 1996-07-19 1998-01-29 Hybridon, Inc. Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
US5922608A (en) 1996-07-31 1999-07-13 Beckman Instruments, Inc. Macromolecule sequencing packet and method of use
US6136212A (en) 1996-08-12 2000-10-24 The Regents Of The University Of Michigan Polymer-based micromachining for microfluidic devices
AU4236597A (en) 1996-08-27 1998-03-19 Visible Genetics Inc. Apparatus and method for performing sequencing of nucleic acid polymers
US5832165A (en) 1996-08-28 1998-11-03 University Of Utah Research Foundation Composite waveguide for solid phase binding assays
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US6020457A (en) 1996-09-30 2000-02-01 Dendritech Inc. Disulfide-containing dendritic polymers
US5965446A (en) 1996-10-24 1999-10-12 Hamamatsu Photonics K.K. Method for placing fluorescent single molecules on surface of substrate and method for visualizing structural defect of surface of substrate
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6002471A (en) 1996-11-04 1999-12-14 California Institute Of Technology High resolution scanning raman microscope
CA2702219C (en) 1996-11-06 2013-01-08 Sequenom, Inc. High density immobilization of nucleic acids
EP1164203B1 (de) 1996-11-06 2007-10-10 Sequenom, Inc. DNA-Diagnostik mittels Massenspektrometrie
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
CA2267070A1 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Compositions and methods for immobilizing nucleic acids to solid supports
US6024925A (en) 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6225062B1 (en) 1996-11-12 2001-05-01 Visible Genetics Inc. Method and kit for direct isothermal sequencing of nucleic acids
US5971355A (en) 1996-11-27 1999-10-26 Xerox Corporation Microdevice valve structures to fluid control
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6017702A (en) 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US5876934A (en) 1996-12-18 1999-03-02 Pharmacia Biotech Inc. DNA sequencing method
DE19653494A1 (de) 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
DE19653439A1 (de) 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6828094B2 (en) 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
WO1998033939A1 (fr) 1997-01-31 1998-08-06 Hitachi, Ltd. Procede pour determiner une sequence de base d'acide nucleique et appareil correspondant
DE69825601T2 (de) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
US6117973A (en) 1997-02-24 2000-09-12 Georgia Tech Research Corp. PNA monomers with electron donor or acceptor
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US6117634A (en) 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
WO1998040520A1 (en) 1997-03-14 1998-09-17 Hybridon, Inc. Method for sequencing of modified nucleic acids using electrospray ionization-fourier transform mass spectrometry
WO1998041657A1 (en) 1997-03-20 1998-09-24 Affymetrix, Inc. Iterative resequencing
AU6846798A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid sequencing
AU746892B2 (en) 1997-04-04 2002-05-02 Caliper Life Sciences, Inc. Closed-loop biochemical analyzers
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
CA2291180A1 (en) 1997-05-23 1998-11-26 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US5945284A (en) 1997-05-27 1999-08-31 The Perkin-Elmer Corporation Length determination of nucleic acid repeat sequences by discontinuous primer extension
SE9702008D0 (sv) 1997-05-28 1997-05-28 Pharmacia Biotech Ab A method and a system for nucleic acid seouence analysis
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
AU8224998A (en) 1997-07-04 1999-01-25 Nycomed Amersham Plc Peroxidase-catalysed fluorescence
CN1152140C (zh) 1997-07-28 2004-06-02 医疗生物系统有限公司 核酸序列分析
EP1037947B1 (de) 1997-07-28 2003-09-10 Amersham plc Cyaninfarbstoffe
US6245506B1 (en) 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
GB9716231D0 (en) 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
DE69824736T2 (de) 1997-08-12 2004-10-21 Eastman Chem Co Acrylmodifizierte wässrige alkyddispersionen
WO1999009616A1 (en) 1997-08-20 1999-02-25 Berg Technology, Inc. High speed modular electrical connector and receptacle for use therein
US5994085A (en) 1997-08-26 1999-11-30 Cantor; Thomas L. Methods and devices for detecting non-complexed prostate specific antigen
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
WO1999013110A1 (en) 1997-09-11 1999-03-18 Seq, Ltd. Method to make fluorescent nucleotide photoproducts for dna sequencing and analysis
WO1999013109A1 (en) 1997-09-11 1999-03-18 Seq, Ltd. Method for dna sequencing analysis
US6346379B1 (en) 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
TW352471B (en) 1997-09-20 1999-02-11 United Microelectronics Corp Method for preventing B-P-Si glass from subsiding
US5836750A (en) 1997-10-09 1998-11-17 Honeywell Inc. Electrostatically actuated mesopump having a plurality of elementary cells
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6049380A (en) 1997-11-12 2000-04-11 Regents Of The University Of California Single molecule identification using selected fluorescence characteristics
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6407858B1 (en) 1998-05-14 2002-06-18 Genetic Microsystems, Inc Focusing of microscopes and reading of microarrays
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6262838B1 (en) 1998-03-20 2001-07-17 Genetic Microsystems Inc Focusing in microscope systems
JP4317953B2 (ja) 1998-01-22 2009-08-19 独立行政法人理化学研究所 Dnaの塩基配列決定方法
WO1999037810A1 (en) 1998-01-23 1999-07-29 Amersham Pharmacia Biotech, Inc. A method, reagent solution and kits for dna sequencing
JP2002501760A (ja) 1998-02-02 2002-01-22 アマーシャム・ファルマシア・バイオテック・アクチボラグ 核酸解析方法
CA2319777C (en) 1998-02-04 2008-01-08 Amersham Pharmacia Biotech, Inc. Dideoxy dye terminators
US6150147A (en) 1998-02-06 2000-11-21 Affymetrix, Inc. Biological array fabrication methods with reduction of static charge
IL123256A0 (en) 1998-02-10 1998-09-24 Yeda Res & Dev Methods for dna amplification and sequencing
WO1999044045A1 (en) 1998-02-27 1999-09-02 Massachusetts Institute Of Technology Single molecule detection with surface-enhanced raman scattering and applications in dna or rna sequencing
CA2324248A1 (en) 1998-03-18 1999-09-23 Amersham Pharmacia Biotech Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus
GB9805918D0 (en) 1998-03-19 1998-05-13 Nycomed Amersham Plc Sequencing by hybridisation
US6185030B1 (en) 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6719868B1 (en) 1998-03-23 2004-04-13 President And Fellows Of Harvard College Methods for fabricating microfluidic structures
WO1999049082A2 (en) 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
DE29806082U1 (de) 1998-04-02 1998-06-18 Ideal Electronics Inc Kühleinrichtung für eine zentrale Recheneinheit
CA2328881A1 (en) 1998-04-16 1999-10-21 Northeastern University Expert system for analysis of dna sequencing electropherograms
US5945325A (en) 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
WO1999057321A1 (en) 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6130046A (en) 1998-05-04 2000-10-10 Affymetrix, Inc. Techniques for synthesis integrity evaluation utilizing cycle fidelity probes
US6261848B1 (en) 1998-05-08 2001-07-17 The Johns Hopkins University Miniature immuno-optical rapid analyte sensor platform
CA2333201A1 (en) 1998-05-22 1999-12-02 California Institute Of Technology Microfabricated cell sorter
JPH11352409A (ja) 1998-06-05 1999-12-24 Olympus Optical Co Ltd 蛍光検出装置
WO1999064840A1 (en) 1998-06-09 1999-12-16 Caliper Technologies Corp. Fluorescent polarization detection in microfluidic systems
US6287821B1 (en) 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
US6479267B1 (en) 1998-06-17 2002-11-12 Amersham Pharmacia Biotech, Inc. FY7 polymerase
GB9813216D0 (en) 1998-06-18 1998-08-19 Pyrosequencing Ab Reaction monitoring systems
CA2321821A1 (en) 1998-06-26 2000-01-06 Visible Genetics Inc. Method for sequencing nucleic acids with reduced errors
US6235473B1 (en) 1998-07-02 2001-05-22 Orchid Biosciences, Inc. Gene pen devices for array printing
US7399844B2 (en) 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20040106110A1 (en) 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
AU5479599A (en) 1998-08-11 2000-03-06 Caliper Technologies Corporation Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6306643B1 (en) 1998-08-24 2001-10-23 Affymetrix, Inc. Methods of using an array of pooled probes in genetic analysis
AU9505798A (en) 1998-09-24 2000-04-10 Biotraces, Inc. Sequencing duplex dna by mass spectroscopy
AU6504099A (en) * 1998-09-29 2000-04-17 Diversa Corporation Nucleic acids and proteins from Cenarchaeum symbiosum
DE19844931C1 (de) 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6607888B2 (en) 1998-10-20 2003-08-19 Wisconsin Alumni Research Foundation Method for analyzing nucleic acid reactions
DE19849348A1 (de) 1998-10-26 2000-04-27 Univ Ludwigs Albert Linear Amplification mediated PCR (=LAM PCR)
US6545264B1 (en) 1998-10-30 2003-04-08 Affymetrix, Inc. Systems and methods for high performance scanning
RU2143343C1 (ru) 1998-11-03 1999-12-27 Самсунг Электроникс Ко., Лтд. Микроинжектор и способ изготовления микроинжектора
US6309701B1 (en) 1998-11-10 2001-10-30 Bio-Pixels Ltd. Fluorescent nanocrystal-labeled microspheres for fluorescence analyses
US6387982B1 (en) 1998-11-23 2002-05-14 Dentsply Detrey G.M.B.H. Self etching adhesive primer composition and polymerizable surfactants
US6245518B1 (en) 1998-12-11 2001-06-12 Hyseq, Inc. Polynucleotide arrays and methods of making and using the same
WO2000036152A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
CA2354682A1 (en) 1998-12-18 2000-06-29 The Regents Of The University Of California Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
US6340750B1 (en) 1998-12-18 2002-01-22 The Texas A&M University System Through bond energy transfer in fluorescent dyes for labelling biological molecules
GB9828785D0 (en) 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
EP2145963A1 (de) 1999-01-06 2010-01-20 Callida Genomics, Inc. Verbesserte Sequenzierung mittels Hybridisierung durch Verwendung von Sondengemischen
US6361671B1 (en) 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
WO2000042223A1 (en) 1999-01-15 2000-07-20 Myriad Genetics, Inc. Method for controlling the distribution of dna sequencing termination products
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US6270644B1 (en) 1999-01-27 2001-08-07 Affymetrix, Inc. Capillary array electrophoresis scanner
DE60044490D1 (de) 1999-02-23 2010-07-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
WO2000050871A1 (en) 1999-02-26 2000-08-31 Orchid Biosciences, Inc. Microstructures for use in biological assays and reactions
US6558945B1 (en) 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
DK1159453T3 (da) 1999-03-10 2008-10-06 Asm Scient Inc Fremgangsmåde til direkte nukleinsyresekventering
WO2000056937A2 (en) 1999-03-25 2000-09-28 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
EP1165839A2 (de) 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
US6403317B1 (en) 1999-03-26 2002-06-11 Affymetrix, Inc. Electronic detection of hybridization on nucleic acid arrays
AU3567900A (en) 1999-03-30 2000-10-16 Solexa Ltd. Polynucleotide sequencing
US6261775B1 (en) 1999-04-09 2001-07-17 The Regents Of The University Of California Detection of chromosome copy number changes to distinguish melanocytic nevi from malignant melanoma
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
US6368562B1 (en) 1999-04-16 2002-04-09 Orchid Biosciences, Inc. Liquid transportation system for microfluidic device
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US20020142329A1 (en) 1999-04-30 2002-10-03 Aclara Biosciences, Inc. Compositions and methods employing cleavable electrophoretic tag reagents
US6673550B2 (en) 1999-04-30 2004-01-06 Aclara Biosciences, Inc. Electrophoretic tag reagents comprising fluorescent compounds
US6395559B1 (en) 1999-05-04 2002-05-28 Orchid Biosciences, Inc. Multiple fluid sample processor with single well addressability
US7655443B1 (en) 1999-05-07 2010-02-02 Siemens Healthcare Diagnostics, Inc. Nucleic acid sequencing with simultaneous quantitation
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423273B1 (en) 1999-05-19 2002-07-23 Orchid Biosciences, Inc. Method of forming seals for a microfluidic device
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6515120B1 (en) 1999-05-25 2003-02-04 Praelux Incorporated Method for sequencing and characterizing polymeric biomolecules using aptamers and a method for producing aptamers
US6485690B1 (en) 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
US6225109B1 (en) 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US6309886B1 (en) 1999-06-04 2001-10-30 The Regents Of The University Of California High throughput analysis of samples in flowing liquid
AU5497100A (en) 1999-06-19 2001-01-09 Hyseq, Inc. Improved methods of sequence assembly in sequencing by hybridization
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001001025A2 (en) 1999-06-28 2001-01-04 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6444106B1 (en) 1999-07-09 2002-09-03 Orchid Biosciences, Inc. Method of moving fluid in a microfluidic device
US6448090B1 (en) 1999-07-09 2002-09-10 Orchid Biosciences, Inc. Fluid delivery system for a microfluidic device using alternating pressure waveforms
US6444173B1 (en) 1999-07-09 2002-09-03 Orchid Biosciences, Inc. Method of moving and detecting fluid in a microfluidic device
US6268219B1 (en) 1999-07-09 2001-07-31 Orchid Biosciences, Inc. Method and apparatus for distributing fluid in a microfluidic device
US6395232B1 (en) 1999-07-09 2002-05-28 Orchid Biosciences, Inc. Fluid delivery system for a microfluidic device using a pressure pulse
AU6095000A (en) 1999-07-13 2001-01-30 Whitehead Institute For Biomedical Research Generic sbe-fret protocol
US20020045182A1 (en) 1999-07-16 2002-04-18 Lynx Therapeutics, Inc. Multiplexed differential displacement for nucleic acid determinations
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
AU7086800A (en) 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
US6528258B1 (en) 1999-09-03 2003-03-04 Lifebeam Technologies, Inc. Nucleic acid sequencing using an optically labeled pore
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6107061A (en) 1999-09-18 2000-08-22 The Perkin-Elmer Corporation Modified primer extension reactions for polynucleotide sequence detection
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
GB9923324D0 (en) 1999-10-01 1999-12-08 Pyrosequencing Ab Separation apparatus and method
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
EP1096024A1 (de) 1999-10-28 2001-05-02 Remacle, José Verfahren und Reagentiensatz zum Auswählen und/oder Quantifizieren von multiplen homologen Nukleinsäuresequenzen unter Verwendung von Sondenanordnungen
EP1228244A4 (de) 1999-11-04 2005-02-09 California Inst Of Techn Verfahren und apparate zur analyse von polynukleotidsequenzen
WO2001038546A1 (en) 1999-11-23 2001-05-31 Amersham Biosciences Corp Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
EP1144689A1 (de) 1999-11-26 2001-10-17 Bioneer Corporation; Dna sequenzierungsmethode die verschiedene dna polymerasen verwendet und entsprechender kit
US6383749B2 (en) 1999-12-02 2002-05-07 Clontech Laboratories, Inc. Methods of labeling nucleic acids for use in array based hybridization assays
GB9929381D0 (en) 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
US20030186256A1 (en) 1999-12-23 2003-10-02 Achim Fischer Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
EP1198596A1 (de) 2000-02-15 2002-04-24 Lynx Therapeutics, Inc. Datenanalyse und anzeigesystem für die dna-sequenzierung auf der basis von ligation
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
JP3442338B2 (ja) 2000-03-17 2003-09-02 株式会社日立製作所 Dna分析装置、dna塩基配列決定装置、dna塩基配列決定方法、および反応モジュール
AU2001254771A1 (en) 2000-04-03 2001-10-15 Axaron Bioscience Ag Novel method for the parallel sequencing of a nucleic acid mixture on a surface
US6436641B1 (en) 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
WO2001083814A2 (en) 2000-05-04 2001-11-08 Syngenta Participations Ag Assay for nucleic acid analysis
US6342326B1 (en) 2000-05-10 2002-01-29 Beckman Coulter, Inc. Synthesis and use of acyl fluorides of cyanine dyes
WO2001085991A2 (de) 2000-05-12 2001-11-15 Gnothis Holding Sa Verfahren zum nachweis von polynukleotiden durch fluoreszenzkorrelationsspektroskopie
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US20020168678A1 (en) 2000-06-07 2002-11-14 Li-Cor, Inc. Flowcell system for nucleic acid sequencing
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
AU2001268468A1 (en) 2000-06-13 2001-12-24 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
US6829753B2 (en) 2000-06-27 2004-12-07 Fluidigm Corporation Microfluidic design automation method and system
DE10031842A1 (de) 2000-06-30 2002-01-31 Gnothis Holding Sa Ecublens Multiplex-Sequenzierungsverfahren
GB0016258D0 (en) 2000-07-03 2000-08-23 Amersham Pharm Biotech Uk Ltd Base analogues
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
CN100462433C (zh) 2000-07-07 2009-02-18 维西根生物技术公司 实时序列测定
US20030017461A1 (en) 2000-07-11 2003-01-23 Aclara Biosciences, Inc. Tag cleavage for detection of nucleic acids
US6397150B1 (en) 2000-07-27 2002-05-28 Visible Genetics Inc. Method and apparatus for sequencing of DNA using an internal calibrant
JP2004512533A (ja) 2000-08-22 2004-04-22 アフィメトリックス インコーポレイテッド 生物学的マイクロアレイスキャナを制御するためのシステム、方法およびコンピュータソフトウェアプロダクト
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0022069D0 (en) 2000-09-08 2000-10-25 Pyrosequencing Ab Method
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
JP3638516B2 (ja) 2000-09-28 2005-04-13 株式会社日立製作所 核酸検出方法および核酸検出キット
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
AU2002211389A1 (en) 2000-10-03 2002-04-15 California Institute Of Technology Microfluidic devices and methods of use
EP1790736A3 (de) * 2000-10-06 2007-08-15 The Trustees Of Columbia University In The City Of New York Massives paralleles Verfahren zum Entschlüsseln von DNA und RNA
EP2072526B1 (de) * 2000-10-06 2012-05-30 Novozymes, Inc. Methoden zur Abtrennung eines PGA Synthese beteiligten Gens in Bacillus licheniformis
AU1189702A (en) 2000-10-13 2002-04-22 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
US20020115092A1 (en) 2000-11-08 2002-08-22 The Scripps Research Institute Energy transfer labels with mechanically linked fluorophores
US6770472B2 (en) 2000-11-17 2004-08-03 The Board Of Trustees Of The Leland Stanford Junior University Direct DNA sequencing with a transcription protein and a nanometer scale electrometer
EP1339876A2 (de) 2000-11-28 2003-09-03 Promega Corporation Aufreinigung von dna sequenzierungreaktionen unter verwendung magnetischer silika-partikel
AU2002217660A1 (en) 2001-01-12 2002-07-24 Karolinska Innovations Ab Substrate for fluorescence analysis
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
WO2002061112A2 (en) 2001-01-29 2002-08-08 The University Of Tennessee Research Corporation Methods for detection of incorporation of a nucleotide onto a nucleic acid primer
ATE374259T1 (de) 2001-01-30 2007-10-15 Solexa Ltd Herstellung von matrizen aus polynukleotiden
US7026163B1 (en) * 2001-02-23 2006-04-11 Mayo Foundation For Medical Education And Research Sulfotransferase sequence variants
US7297518B2 (en) * 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20040038206A1 (en) 2001-03-14 2004-02-26 Jia Zhang Method for high throughput assay of genetic analysis
DE10115309A1 (de) 2001-03-28 2002-10-02 Gnothis Holding Sa Ecublens Mikroskopanordnung zur Fluoreszenzspektorskopie, insbesondere Fluoreszenzkorrelationsspektroskopie
US20030027140A1 (en) 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
DE10120797B4 (de) 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
DE10120798B4 (de) 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
CN1384203A (zh) 2001-04-30 2002-12-11 香港科技创业股份有限公司 具有高度延伸专一性的dna低温循环延伸反应方法
US20020172948A1 (en) 2001-05-04 2002-11-21 Perlin Mark W. Method and system for nucleic acid sequencing
JP2004537292A (ja) * 2001-05-25 2004-12-16 ディーエヌエープリント ジェノミクス インコーポレーティッド 体色形質を推測するための組成物および方法
US20050260570A1 (en) 2001-05-29 2005-11-24 Mao Jen-I Sequencing by proxy
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US6689478B2 (en) 2001-06-21 2004-02-10 Corning Incorporated Polyanion/polycation multilayer film for DNA immobilization
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US6989267B2 (en) 2001-07-02 2006-01-24 Agilent Technologies, Inc. Methods of making microarrays with substrate surfaces having covalently bound polyelectrolyte films
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US6995841B2 (en) 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
AU2002337030A1 (en) 2001-08-29 2003-03-18 Genovoxx Gmbh Method for analyzing nucleic acid sequences and gene expression
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
EP1438435B1 (de) 2001-08-31 2007-10-31 Datascope Investment Corp. Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen
US20030104386A1 (en) 2001-08-31 2003-06-05 The Regents Of The University Of California Methods for the specific detection of redox-active tags and the use thereof for capillary gel electrophoresis and DNA sequencing
US6982165B2 (en) 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6975943B2 (en) 2001-09-24 2005-12-13 Seqwright, Inc. Clone-array pooled shotgun strategy for nucleic acid sequencing
DE10246005A1 (de) 2001-10-04 2003-04-30 Genovoxx Gmbh Gerät zur Sequenzierung von Nukleinsäuremolekülen
US7406385B2 (en) * 2001-10-25 2008-07-29 Applera Corporation System and method for consensus-calling with per-base quality values for sample assemblies
US20040054162A1 (en) 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
WO2003044678A1 (fr) 2001-11-22 2003-05-30 Hitachi, Ltd. Systeme de traitement de donnees utilisant des donnees relatives a une sequence de base
GB0128526D0 (en) 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0200938D0 (en) 2002-01-16 2002-03-06 Solexa Ltd Prism design for scanning applications
US7211382B2 (en) 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
US20040014096A1 (en) 2002-04-12 2004-01-22 Stratagene Dual-labeled nucleotides
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
DE10256898B4 (de) 2002-11-29 2006-01-12 Senslab-Gesellschaft Zur Entwicklung Und Herstellung Bioelektrochemischer Sensoren Mbh Elektrochemischer Nachweis von Nukleinsäuren
US8676383B2 (en) * 2002-12-23 2014-03-18 Applied Biosystems, Llc Device for carrying out chemical or biological reactions
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US8399196B2 (en) 2003-02-21 2013-03-19 Geneform Technologies Limited Nucleic acid sequencing methods, kits and reagents
US20050170367A1 (en) 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
DE602005020421D1 (de) * 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US20100216153A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US20050239085A1 (en) 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US7492462B2 (en) 2006-01-17 2009-02-17 Honeywell International, Inc. Optochemical sensor
US7282337B1 (en) * 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing

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