EA200701662A1 - Способ подготовки полинуклеотидов для анализа - Google Patents

Способ подготовки полинуклеотидов для анализа

Info

Publication number
EA200701662A1
EA200701662A1 EA200701662A EA200701662A EA200701662A1 EA 200701662 A1 EA200701662 A1 EA 200701662A1 EA 200701662 A EA200701662 A EA 200701662A EA 200701662 A EA200701662 A EA 200701662A EA 200701662 A1 EA200701662 A1 EA 200701662A1
Authority
EA
Eurasian Patent Office
Prior art keywords
polynucleotide
target
analysis
hybridized
forming
Prior art date
Application number
EA200701662A
Other languages
English (en)
Other versions
EA012525B1 (ru
Inventor
Пребен Лексоу
Original Assignee
Лингвитае Ас
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Лингвитае Ас filed Critical Лингвитае Ас
Publication of EA200701662A1 publication Critical patent/EA200701662A1/ru
Publication of EA012525B1 publication Critical patent/EA012525B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Предлагается способ анализа целевого полинуклеотида (полинуклеотида-мишени), содержащего различающиеся элементы нуклеотидной последовательности, включающий: (i) формирование первичного полинуклеотида, представляющего собой конкатемер, имеющий несколько повторяющихся последовательностей целевого полинуклеотида (полинуклеотида-мишени); (ii) формирование на первичном полинуклеотиде вторичного полинуклеотида, гибридизованного с частью одного или более из полинукпеотидов-мишеней, так что гибридизованная часть или негибридизованная часть соответствует элементу последовательности мишени, и определение на мишени элемента последовательности.
EA200701662A 2005-03-08 2006-03-08 Способ подготовки полинуклеотидов для анализа EA012525B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0504774.1A GB0504774D0 (en) 2005-03-08 2005-03-08 Method
PCT/GB2006/000825 WO2006095169A1 (en) 2005-03-08 2006-03-08 Method for preparing polynucleotides for analysis

Publications (2)

Publication Number Publication Date
EA200701662A1 true EA200701662A1 (ru) 2008-04-28
EA012525B1 EA012525B1 (ru) 2009-10-30

Family

ID=34452026

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200701662A EA012525B1 (ru) 2005-03-08 2006-03-08 Способ подготовки полинуклеотидов для анализа

Country Status (13)

Country Link
US (1) US20090053699A1 (ru)
EP (1) EP1861510A1 (ru)
JP (1) JP2008532508A (ru)
KR (1) KR20080005196A (ru)
CN (1) CN101142325A (ru)
AU (1) AU2006221770A1 (ru)
BR (1) BRPI0608288A2 (ru)
CA (1) CA2600240A1 (ru)
EA (1) EA012525B1 (ru)
GB (1) GB0504774D0 (ru)
NO (1) NO20074962L (ru)
WO (1) WO2006095169A1 (ru)
ZA (1) ZA200707704B (ru)

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US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2970960B1 (en) 2013-03-15 2019-03-13 Theranos IP Company, LLC Nucleic acid amplification
WO2014145128A2 (en) * 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
WO2014145291A1 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nucleic acid amplification
BR112015023398A8 (pt) 2013-03-15 2022-08-23 Theranos Inc Métodos para amplificar ácidos nucléicos
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
US9868979B2 (en) * 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
MX2018005873A (es) 2013-09-06 2022-11-17 Labrador Diagnostics Llc Sistemas y metodos para detectar enfermedades infecciosas.
BR112016011692A2 (pt) * 2013-11-22 2017-09-26 Theranos Inc amplificação de ácido nucléico
EP3218519B1 (en) * 2014-11-11 2020-12-02 BGI Shenzhen Multi-pass sequencing
ES2955916T3 (es) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Análisis múltiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
JP6867045B2 (ja) * 2015-08-12 2021-04-28 ザ チャイニーズ ユニバーシティ オブ ホンコン 血漿ｄｎａの単分子配列決定
EP3894592A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating spatial arrays with gradients
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
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WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

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EP0542874A4 (en) * 1990-07-25 1994-05-11 Syngene Inc Circular extension for generating multiple nucleic acid complements
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
DE69627698T2 (de) * 1995-12-05 2004-01-15 Jorn Erland Koch Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
GB0308851D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method

Also Published As

Publication number Publication date
CN101142325A (zh) 2008-03-12
BRPI0608288A2 (pt) 2009-12-22
GB0504774D0 (en) 2005-04-13
ZA200707704B (en) 2008-12-31
NO20074962L (no) 2007-11-23
AU2006221770A1 (en) 2006-09-14
EA012525B1 (ru) 2009-10-30
CA2600240A1 (en) 2006-09-14
US20090053699A1 (en) 2009-02-26
KR20080005196A (ko) 2008-01-10
EP1861510A1 (en) 2007-12-05
WO2006095169A1 (en) 2006-09-14
JP2008532508A (ja) 2008-08-21

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