AU5479599A - Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes - Google Patents

Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes

Info

Publication number
AU5479599A
AU5479599A AU54795/99A AU5479599A AU5479599A AU 5479599 A AU5479599 A AU 5479599A AU 54795/99 A AU54795/99 A AU 54795/99A AU 5479599 A AU5479599 A AU 5479599A AU 5479599 A AU5479599 A AU 5479599A
Authority
AU
Australia
Prior art keywords
distinguishing
systems
methods
fluorescent probes
decay times
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU54795/99A
Inventor
Morten J. Jensen
J. Wallace Parce
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/213,297 external-priority patent/US6447724B1/en
Application filed by Caliper Technologies Corp filed Critical Caliper Technologies Corp
Publication of AU5479599A publication Critical patent/AU5479599A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
AU54795/99A 1998-08-11 1999-08-11 Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes Abandoned AU5479599A (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US13218198A 1998-08-11 1998-08-11
US13255498A 1998-08-11 1998-08-11
US12206498P 1998-08-11 1998-08-11
US09132554 1998-08-11
US09132181 1998-08-11
US60122064 1998-08-11
US09213297 1998-12-15
US09/213,297 US6447724B1 (en) 1998-08-11 1998-12-15 DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
PCT/US1999/018294 WO2000009753A1 (en) 1998-08-11 1999-08-11 Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes

Publications (1)

Publication Number Publication Date
AU5479599A true AU5479599A (en) 2000-03-06

Family

ID=27494406

Family Applications (1)

Application Number Title Priority Date Filing Date
AU54795/99A Abandoned AU5479599A (en) 1998-08-11 1999-08-11 Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes

Country Status (3)

Country Link
EP (1) EP1104491A4 (en)
AU (1) AU5479599A (en)
WO (1) WO2000009753A1 (en)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1084391A4 (en) 1998-06-08 2006-06-14 Caliper Life Sciences Inc Microfluidic devices, systems and methods for performing integrated reactions and separations
EP1163052B1 (en) 1999-02-23 2010-06-02 Caliper Life Sciences, Inc. Manipulation of microparticles in microfluidic systems
AU770678B2 (en) 1999-05-17 2004-02-26 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6649358B1 (en) 1999-06-01 2003-11-18 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
EP1222257A4 (en) 1999-10-08 2007-12-12 Caliper Life Sciences Inc Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
US6468761B2 (en) 2000-01-07 2002-10-22 Caliper Technologies, Corp. Microfluidic in-line labeling method for continuous-flow protease inhibition analysis
US7037416B2 (en) 2000-01-14 2006-05-02 Caliper Life Sciences, Inc. Method for monitoring flow rate using fluorescent markers
US6556923B2 (en) * 2000-01-26 2003-04-29 Caliper Technologies Corp. Software for high throughput microfluidic systems
US20020012971A1 (en) 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
US6733645B1 (en) 2000-04-18 2004-05-11 Caliper Technologies Corp. Total analyte quantitation
CA2406718A1 (en) 2000-05-11 2001-11-15 Caliper Technologies Corp. Microfluidic devices and methods to regulate hydrodynamic and electrical resistance utilizing bulk viscosity enhancers
EP1297179B1 (en) 2000-05-12 2008-09-24 Caliper Life Sciences, Inc. Detection of nucleic acid hybridization by fluorescence polarization
AU8470001A (en) 2000-08-03 2002-02-18 Caliper Techn Corp Methods and devices for high throughput fluid delivery
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (en) 2000-10-06 2007-03-15 Univ Columbia MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA
US20090118139A1 (en) 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
WO2002103210A1 (en) * 2001-06-15 2002-12-27 Hansford Derek J Nanopump devices and methods
EP1424957A2 (en) 2001-09-14 2004-06-09 Francis J. Martin Microfabricated nanopore device for sustained release of therapeutic agent
US20040126765A1 (en) * 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
DE112007002932B4 (en) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable, reversible, fluorescent nucleotide terminators
DK2725107T3 (en) 2007-10-19 2019-01-02 Univ Columbia DNA Sequencing with Non-Fluorescent Nucleotide Reversible Terminators and ddNTPs Modified by Split Label and Nucleic Acid comprising Inosine with Reversible Terminators
EP2207900B1 (en) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
CA3026061A1 (en) * 2016-06-01 2017-12-07 Quantum-Si Incorporated Pulse caller and base caller
AU2019211435A1 (en) 2018-01-26 2020-07-30 Quantum-Si Incorporated Machine learning enabled pulse and base calling for sequencing devices

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849513A (en) * 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
US5171534A (en) * 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
SE8802573D0 (en) * 1988-07-08 1988-07-08 Wallac Oy MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES
JP2821050B2 (en) * 1991-10-31 1998-11-05 浜松ホトニクス株式会社 Nucleic acid base identification method
IL108497A0 (en) * 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
FI93781C (en) * 1993-03-18 1995-05-26 Wallac Oy Biospecific multiparametric assay method
FI101829B1 (en) * 1995-03-07 1998-08-31 Erkki Juhani Soini Biospecific assay method
DE19634873A1 (en) * 1996-08-29 1998-03-12 Boehringer Mannheim Gmbh System for the differentiation of fluorescent groups of molecules by time-resolved fluorescence measurement
US6284462B1 (en) * 1996-09-27 2001-09-04 Laboratory Of Molecular Biophotonics Probes and methods for polynucleotide detection

Also Published As

Publication number Publication date
EP1104491A4 (en) 2003-01-29
WO2000009753A1 (en) 2000-02-24
EP1104491A1 (en) 2001-06-06

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase