SE8802573D0 - MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES - Google Patents

MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES

Info

Publication number
SE8802573D0
SE8802573D0 SE8802573A SE8802573A SE8802573D0 SE 8802573 D0 SE8802573 D0 SE 8802573D0 SE 8802573 A SE8802573 A SE 8802573A SE 8802573 A SE8802573 A SE 8802573A SE 8802573 D0 SE8802573 D0 SE 8802573D0
Authority
SE
Sweden
Prior art keywords
different
label
lanthanide
ligand
nucleic acid
Prior art date
Application number
SE8802573A
Other languages
Swedish (sv)
Inventor
M Kwiatkowski
E Soini
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy filed Critical Wallac Oy
Priority to SE8802573A priority Critical patent/SE8802573D0/en
Publication of SE8802573D0 publication Critical patent/SE8802573D0/en
Priority to PCT/SE1989/000378 priority patent/WO1990000623A1/en
Priority to EP89907852A priority patent/EP0386180A1/en
Priority to JP1507291A priority patent/JPH03500249A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C237/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups
    • C07C237/28—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a non-condensed six-membered aromatic ring of the carbon skeleton
    • C07C237/36—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a non-condensed six-membered aromatic ring of the carbon skeleton having the nitrogen atom of the carboxamide group bound to an acyclic carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D213/44—Radicals substituted by doubly-bound oxygen, sulfur, or nitrogen atoms, or by two such atoms singly-bound to the same carbon atom
    • C07D213/46—Oxygen atoms
    • C07D213/50—Ketonic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/62—Oxygen or sulfur atoms
    • C07D213/63—One oxygen atom
    • C07D213/65—One oxygen atom attached in position 3 or 5

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention pertains to a method for simultaneous real time fluorometric sequence analysis of nucleic acid fragments of different lengths and four different terminals, the fragments being labelled with different fluorescent labels for the respective terminating bases. The fragments are labelled with four different labels comprising the same two lanthanides and the same two ligands as follows: label 1 = ligand 1 + lanthanide 1, label 2 = ligand 2 + lanthanide 1, label 3 = ligand 1 + lanthanide 2, label 4 = ligand 2 + lanthanide 2, where ligands 1 and 2 have different excitation wavelengths and lanthanides 1 and 2 have different emission wavelengths, excitation light is generated in the form of light pulses alternating between the different excitation wavelengths of the ligands, and the photons emitted by the labels are detected periodically for the different emission wavelengths of the lanthanides.
SE8802573A 1988-07-08 1988-07-08 MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES SE8802573D0 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
SE8802573A SE8802573D0 (en) 1988-07-08 1988-07-08 MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES
PCT/SE1989/000378 WO1990000623A1 (en) 1988-07-08 1989-07-03 Multi-label time-resolved fluorescence analysis of nucleic acid sequences using lanthanide chelates
EP89907852A EP0386180A1 (en) 1988-07-08 1989-07-03 Multi-label time-resolved fluorescence analysis of nucleic acid sequences using lanthanide chelates
JP1507291A JPH03500249A (en) 1988-07-08 1989-07-03 Multiplexed label time-resolved fluorescence analysis of nucleic acid sequences using lanthanide chelates

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8802573A SE8802573D0 (en) 1988-07-08 1988-07-08 MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES

Publications (1)

Publication Number Publication Date
SE8802573D0 true SE8802573D0 (en) 1988-07-08

Family

ID=20372870

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8802573A SE8802573D0 (en) 1988-07-08 1988-07-08 MULTI-LABEL TIME-RESOLVED FLUORESCENCE ANALYSIS OF NUCLEIC ACID SEQUENCES USING LANTHANIDE CHELATES

Country Status (4)

Country Link
EP (1) EP0386180A1 (en)
JP (1) JPH03500249A (en)
SE (1) SE8802573D0 (en)
WO (1) WO1990000623A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962045A (en) * 1988-05-02 1990-10-09 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
AU638568B2 (en) * 1990-02-26 1993-07-01 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A method for the fluorescent detection of a dna sequence in real time
CA2079448C (en) * 1991-02-14 2000-05-23 David Gordon Sherman Lanthanide chelate-conjugated oligonucleotides
GB2264496B (en) * 1992-02-25 1995-10-25 Us Energy Sizing of fragments from a nucleic acid sequence
JP4321737B2 (en) * 1997-12-17 2009-08-26 塩野義製薬株式会社 New pyridine compounds
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
AU5479599A (en) * 1998-08-11 2000-03-06 Caliper Technologies Corporation Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
US20010055763A1 (en) * 1998-12-24 2001-12-27 Sharat Singh Individually addressable solid surfaces for multiplexed operations
US6329205B1 (en) 1999-08-31 2001-12-11 Molecular Probes, Inc. Detection method using luminescent europium-based protein stains
US7482444B2 (en) 2006-03-13 2009-01-27 Wallac Oy Terminating substrates for DNA polymerases
FI20065162A0 (en) * 2006-03-13 2006-03-13 Wallac Oy Terminating substrates for DNA polymerases
RU2767695C2 (en) 2012-03-16 2022-03-18 Стат-Диагностика Энд Инновэйшн, С.Л. Testing cassette with built-in transmitting module

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2155176B (en) * 1984-01-16 1988-05-11 California Inst Of Techn Sequencing dna
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
GB8626575D0 (en) * 1986-11-06 1986-12-10 Amersham Int Plc Biochemical sequencing

Also Published As

Publication number Publication date
EP0386180A1 (en) 1990-09-12
JPH03500249A (en) 1991-01-24
WO1990000623A1 (en) 1990-01-25

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