CN102725407B - 分离核酸的方法及其试剂盒 - Google Patents

分离核酸的方法及其试剂盒 Download PDF

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CN102725407B
CN102725407B CN201180005674.5A CN201180005674A CN102725407B CN 102725407 B CN102725407 B CN 102725407B CN 201180005674 A CN201180005674 A CN 201180005674A CN 102725407 B CN102725407 B CN 102725407B
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cotton
nucleic acid
buffer
water
solution
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CN102725407A (zh
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帕尼·库马尔·普莱拉
穆拉克卡普尔斯·纳拉亚南·马努基
桑托什·库马尔·甘达瓦拉
米切尔·普里坦·平托
钱德拉塞卡尔·巴斯卡拉·奈尔
皮拉里斯蒂·文卡塔·苏巴拉奥
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Bigtec Pvt Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180005674.5A 2010-01-07 2011-01-06 分离核酸的方法及其试剂盒 Active CN102725407B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN50/CHE/2010 2010-01-07
IN50CH2010 2010-01-07
PCT/IB2011/050044 WO2011083429A1 (fr) 2010-01-07 2011-01-06 Procédé pour isoler des acides nucléiques, et kit à cet effet

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CN102725407A CN102725407A (zh) 2012-10-10
CN102725407B true CN102725407B (zh) 2016-09-14

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US (1) US8986976B2 (fr)
EP (1) EP2521780B1 (fr)
JP (1) JP5791631B2 (fr)
KR (1) KR101590544B1 (fr)
CN (1) CN102725407B (fr)
BR (1) BR112012016774B1 (fr)
CY (1) CY1119878T1 (fr)
DK (1) DK2521780T3 (fr)
EA (1) EA022842B1 (fr)
ES (1) ES2652335T3 (fr)
HR (1) HRP20171823T1 (fr)
HU (1) HUE034870T2 (fr)
LT (1) LT2521780T (fr)
NO (1) NO2521780T3 (fr)
PL (1) PL2521780T3 (fr)
PT (1) PT2521780T (fr)
RS (1) RS56636B1 (fr)
SG (1) SG182372A1 (fr)
SI (1) SI2521780T1 (fr)
WO (1) WO2011083429A1 (fr)
ZA (1) ZA201205053B (fr)

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DE102011080853B4 (de) * 2011-08-11 2014-03-27 Axagarius Gmbh & Co. Kg Verfahren zur Isolierung von RNA aus Volblutproben
EP2830767A1 (fr) * 2012-03-27 2015-02-04 Northwestern University Récipient et système destinés à la récupération et à la préparation d'échantillon
US9540635B2 (en) * 2012-05-09 2017-01-10 Bio-Rad Laboratories, Inc. Buffer for one-step DNA extraction
CN103571825B (zh) * 2012-08-09 2016-03-16 财团法人工业技术研究院 用于生物样本处理之组合物及使用其之核酸扩增方法
WO2014047141A1 (fr) * 2012-09-19 2014-03-27 Beckman Coulter, Inc. Utilisation d'ions divalents, de protéases et de détergents sous un faible ph pour l'extraction d'acides nucléiques
EP3828270A1 (fr) 2012-09-28 2021-06-02 Cepheid Compositions d'extraction d'adn et d'arn à partir d'échantillons tissulaires fixés incorporés dans de la paraffine
CN103882006A (zh) * 2012-12-19 2014-06-25 河北省健海生物芯片技术有限责任公司 适用于多种样本的dna提取试剂配制方法和提取方法
CN103289990A (zh) * 2013-06-14 2013-09-11 浙江世纪康大医疗科技有限公司 一种血液dna的快速提取方法
WO2015022410A1 (fr) * 2013-08-16 2015-02-19 General Electric Company Procédés et compositions pour l'extraction et la conservation d'acides nucléiques
TWI642679B (zh) * 2014-07-29 2018-12-01 精專生醫股份有限公司 萃取核酸分子之裝置及其使用方法
CN105734044A (zh) * 2014-12-08 2016-07-06 苏州新波生物技术有限公司 用于核酸提取纯化的漂洗液
CN104911176A (zh) * 2015-01-25 2015-09-16 江苏发士达生物科技有限公司 一种细菌dna提取液、提取方法及其应用
GB201504459D0 (en) * 2015-03-17 2015-04-29 Moorlodge Biotech Ventures Ltd Isolation of DNA
US10465182B2 (en) * 2015-07-24 2019-11-05 Cepheid Compositions and methods for DNA and RNA extraction from tissue samples
CN105624149A (zh) * 2016-01-16 2016-06-01 长春市博坤生物科技有限公司 一种用于高难接触性检材dna提取纯化的试剂盒及方法
CN105420231A (zh) * 2016-01-21 2016-03-23 杭州和壹基因科技有限公司 一种从人体唾液中提取基因组dna的方法
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
SG10202008016YA (en) * 2016-02-23 2020-09-29 Bigtec Private Ltd A cartridge for purifying a sample and analysis
RU2628695C1 (ru) * 2016-06-14 2017-08-21 Общество с ограниченной ответственностью "Биологическая среда" Способ выделения РНК и ДНК из сухих биологических образцов, хранившихся на бумажном носителе, и набор для его осуществления
GB2551801A (en) * 2016-06-30 2018-01-03 Lgc Genomics Ltd Methods
RU2650865C1 (ru) * 2016-11-29 2018-04-17 Общество с ограниченной ответственностью "Магнэтик" Набор реактивов для выделения днк
CN106801068B (zh) * 2017-01-12 2020-03-17 西安交通大学 一种自发荧光可降解聚柠檬酸酯的非病毒基因载体及其制备方法
RU2651937C1 (ru) * 2017-05-04 2018-04-24 Татьяна Георгиевна Фалеева Композиция для сбора и хранения днк или днк-содержащих биологических следов (варианты) и её применение
CN109207472B (zh) * 2017-07-06 2023-10-20 上海科华生物工程股份有限公司 Dna病毒核酸提取试剂盒及其使用方法
CN109385418B (zh) * 2017-08-03 2022-07-22 杭州优思达生物技术有限公司 一种用于动物样本中病毒/细菌核酸提取的方法及试剂
EP3688186A4 (fr) * 2017-09-27 2021-10-13 University of North Carolina Wilmington Outil de dépistage d'agents pathogènes dérivés de l'homme et d'eaux usées humaines
CN107723285A (zh) * 2017-10-18 2018-02-23 中山大学肿瘤防治中心 一种通用于常规生物样本的总dna提取方法及提取试剂盒
US20200277650A1 (en) 2017-11-16 2020-09-03 Matjaz Vogelsang Polynucleotide-binding protein for use in diagnosis
CN107904232B (zh) * 2017-12-29 2020-10-16 浙江今复康生物科技有限公司 一种从痰液中快速提取核酸的方法
CN109932359A (zh) * 2019-02-11 2019-06-25 张丽英 一种利用atp生物发光反应检测食品中肉毒杆菌的方法
CN109897851A (zh) * 2019-04-12 2019-06-18 武汉科技大学 小分子dna纯化试剂
CN111826420B (zh) * 2019-04-15 2023-06-23 南京林业大学 松材线虫核酸提取试剂及其应用
CN110029105A (zh) * 2019-05-14 2019-07-19 成都罗宁生物科技有限公司 一种提取微生物dna的试剂盒及其方法
CN113025608A (zh) * 2019-12-09 2021-06-25 深圳市真迈生物科技有限公司 细胞裂解液、试剂盒及应用
CN110938624A (zh) * 2019-12-27 2020-03-31 深圳市海普洛斯生物科技有限公司 一种用于基因组dna提取的试剂盒及其应用
CN111334503A (zh) * 2020-04-07 2020-06-26 江苏康为世纪生物科技有限公司 一种核酸保存液
CN112239775A (zh) * 2020-11-12 2021-01-19 苏州创澜生物科技有限公司 一种用于核酸提取的洗涤缓冲液
CN112176033A (zh) * 2020-11-12 2021-01-05 苏州创澜生物科技有限公司 一种用于rna提取的裂解缓冲液
CN112725413B (zh) * 2021-02-05 2022-10-14 厦门甄准生物技术有限公司 一种宫颈拭子核酸提取的裂解结合液、试剂盒及方法
KR102438028B1 (ko) * 2021-07-15 2022-08-30 주식회사 지엠디바이오텍 휴대용 성분 추출 스포이트 및 성분 추출 방법
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ES2652335T3 (es) 2018-02-01
ZA201205053B (en) 2013-03-27
BR112012016774A2 (pt) 2019-09-10
EP2521780B1 (fr) 2017-08-23
EA201290612A1 (ru) 2013-02-28
PT2521780T (pt) 2017-12-21
LT2521780T (lt) 2018-02-12
BR112012016774B1 (pt) 2020-12-01
US8986976B2 (en) 2015-03-24
KR101590544B1 (ko) 2016-02-18
EA022842B1 (ru) 2016-03-31
NO2521780T3 (fr) 2018-01-20
PL2521780T3 (pl) 2018-03-30
CY1119878T1 (el) 2018-06-27
SI2521780T1 (en) 2018-02-28
CN102725407A (zh) 2012-10-10
WO2011083429A1 (fr) 2011-07-14
SG182372A1 (en) 2012-08-30
RS56636B1 (sr) 2018-03-30
EP2521780A1 (fr) 2012-11-14
JP5791631B2 (ja) 2015-10-07
HUE034870T2 (hu) 2018-03-28
US20130203150A1 (en) 2013-08-08
EP2521780A4 (fr) 2013-07-31
KR20120104625A (ko) 2012-09-21
HRP20171823T1 (hr) 2018-01-12
DK2521780T3 (en) 2017-12-04
JP2013516186A (ja) 2013-05-13

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