SI3002289T1 - Modificirani nukleotidi za sekvenciranje polinukleotida - Google Patents

Modificirani nukleotidi za sekvenciranje polinukleotida Download PDF

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SI3002289T1
SI3002289T1 SI200332563T SI200332563T SI3002289T1 SI 3002289 T1 SI3002289 T1 SI 3002289T1 SI 200332563 T SI200332563 T SI 200332563T SI 200332563 T SI200332563 T SI 200332563T SI 3002289 T1 SI3002289 T1 SI 3002289T1
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nucleotide
group
molecule
fissile
base
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SI200332563T
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John Milton
Xiaolin Wu
Mark Smith
Joseph Brennan
Colin Barnes
Xiaohai Liu
Silke Ruediger
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Illumina Cambridge Limited
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Priority claimed from US10/227,131 external-priority patent/US7057026B2/en
Priority claimed from GB0230037A external-priority patent/GB0230037D0/en
Priority claimed from GB0303924A external-priority patent/GB0303924D0/en
Application filed by Illumina Cambridge Limited filed Critical Illumina Cambridge Limited
Publication of SI3002289T1 publication Critical patent/SI3002289T1/sl

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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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Claims (15)

  1. MODIFICIRANI NUKLEOTIDI ZA SEKVENCIRANJE POLINUKLEOTIDA Patentni zahtevki
    1. Modificirana nukleotid trifosfatna molekula, ki obsega purinsko ali pirimidinsko bazo, in del deoksiriboza sladkoba, ki ima 3'-azidometilno skupino.
  2. 2. Molekula po zahtevku 1, pri čemer je omenjena baza povezana z zaznavno oznako preko cepljivega linkeija ali ne-cepljivega linkeija.
  3. 3. Molekula po zahtevku 2, pri čemer je omenjeni linker cepljiv.
  4. 4. Molekula po zahtevkih 2 ali 3, pri čemer je zaznavna oznaka fluorofor.
  5. 5. Molekula po zahtevkih 3 ali 4, pri čemer omenjeni linker vsebuje fosfin cepljivi azid.
  6. 6. Komplet, ki obsega štiri modificirane nukleotid trifosfatne molekule, vsaka obsegajoča purinsko ali pirimidinsko bazo in del deoksiriboza sladkoija, ki ima 3'-azidometilno skupino, pri čemer ima vsak nukleotid bazo, ki je povezana z zaznavno oznako preko cepljivega linkeija in pri čemer se zaznavna oznaka, povezana z vsakim nukleotidom, lahko razlikuje po detekciji od zaznavne oznake, ki se uporablja za druge tri nukleotide.
  7. 7. Komplet po zahtevku 6, ki dalje vsebuje polimerazo.
  8. 8. Komplet po zahtevku 7, pri čemer je polimeraza Thermococcus sp.
  9. 9. Polinukleotidna molekula, ki ima 3'-azidometilno skupino.
  10. 10. Postopek za določanje sekvence ciljnega enoverižnega polinukleotida, ki obsega spremljanje sekvenčne vključitve komplementarnih nukleotidov, pri čemer je vsaj eno od njih vključevanje nukleotida, ki ima purinsko ali pirimidinsko bazo in del deoksiriboza sladkoija, ki ima 3'-azidometilno skupino, pri čemer ima nukleotid bazo, kije povezana z zaznavno oznako preko cepljivega linkeija in pri čemer se identiteta nukleotida določi z zaznavanjem oznake, ki je povezana z bazo, in se blokima skupina in oznaka odstranita pred uvedbo naslednjega komplementarnega nukleotida.
  11. 11. Postopek po zahtevku 10, pri čemer se oznaka nukleotida in blokima skupina odstranita v enem koraku kemične obdelave.
  12. 12. Postopek po zahtevkih 10 ali 11, pri čemer postopek obsega: (a) zagotovitev množice različnih nukleotidov, pri čemer ima vsak nukleotid množice različnih nukleotidov 3'-azidometilno skupino in bazo, kije vezana na zaznavno oznako preko cepljivega linkeija, pri čemer se zaznavna oznaka, vezana na vsako vrsto nukleotida, lahko razlikuje po detekciji od zaznavne oznake, ki se uporablja za druge vrste nukleotidov; (b) vključitev nukleotida v komplement ciljnega eno verižnega polinukleotida; (c) zaznavanje oznake nukleotida (b), s čimer se določi vrsta vključenega nukleotida; (d) odstranitev oznake nukleotida (b) in blokime skupine; in (e) opcijsko ponovitev korakov (b) - (d) enkrat ali večkrat; s čimer se določi sekvenca ciljnega enoverižnega polinukleotida.
  13. 13. Postopek po kateremkoli od zahtevkov od 10 do 12, pri čemer se blokima skupina odstrani z uporabo vodotopnega fosfina.
  14. 14. Postopek po zahtevku 13, pri čemer je fosfin derivatiziran trialkil fosfin.
  15. 15. Postopek po zahtevku 14, pri čemer je fosfin derivatiziran z eno ali več funkcionalnostmi, izbranimi iz skupine, ki obsega amino, hidroksilne, karboksilne in sulfonatne skupine.
SI200332563T 2002-08-23 2003-08-22 Modificirani nukleotidi za sekvenciranje polinukleotida SI3002289T1 (sl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10/227,131 US7057026B2 (en) 2001-12-04 2002-08-23 Labelled nucleotides
GB0230037A GB0230037D0 (en) 2002-12-23 2002-12-23 Modified nucleotides
GB0303924A GB0303924D0 (en) 2003-02-20 2003-02-20 Modified nucleotides
EP15184520.3A EP3002289B1 (en) 2002-08-23 2003-08-22 Modified nucleotides for polynucleotide sequencing

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SI3002289T1 true SI3002289T1 (sl) 2018-07-31

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SI200332612T SI3363809T1 (sl) 2002-08-23 2003-08-22 Modificirani nukleotidi za polinukleotidno sekvenciranje
SI200332613T SI3587433T1 (sl) 2002-08-23 2003-08-22 Modificirani nukleotidi
SI200332563T SI3002289T1 (sl) 2002-08-23 2003-08-22 Modificirani nukleotidi za sekvenciranje polinukleotida

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SI200332613T SI3587433T1 (sl) 2002-08-23 2003-08-22 Modificirani nukleotidi

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US (9) US7541444B2 (sl)
EP (6) EP3587433B1 (sl)
JP (3) JP2006509040A (sl)
AU (1) AU2003259350A1 (sl)
CY (1) CY1120186T1 (sl)
DK (3) DK3363809T3 (sl)
ES (2) ES2550513T3 (sl)
GB (1) GB2395954A (sl)
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DK3363809T3 (da) 2020-05-04
DK3587433T3 (da) 2020-05-18
JP2014011999A (ja) 2014-01-23
CY1120186T1 (el) 2018-12-12
JP2006509040A (ja) 2006-03-16
EP3363809A1 (en) 2018-08-22
EP3002289A1 (en) 2016-04-06
EP3363809B1 (en) 2020-04-08
US20100292452A1 (en) 2010-11-18
EP3587433B1 (en) 2020-04-22
AU2003259350A8 (en) 2004-03-11
AU2003259350A1 (en) 2004-03-11
DK3002289T3 (en) 2018-04-23
EP3002289B1 (en) 2018-02-28
US9388464B2 (en) 2016-07-12
US7541444B2 (en) 2009-06-02
US7771973B2 (en) 2010-08-10
US20160032378A1 (en) 2016-02-04
US20070166705A1 (en) 2007-07-19
US8071739B2 (en) 2011-12-06
ES2407681T3 (es) 2013-06-13
EP1530578A2 (en) 2005-05-18
JP2011088898A (ja) 2011-05-06
GB2395954A (en) 2004-06-09
WO2004018497A3 (en) 2004-06-17
SI3587433T1 (sl) 2020-08-31
US9121060B2 (en) 2015-09-01
WO2004018497A2 (en) 2004-03-04
EP3587433A1 (en) 2020-01-01
US20160362737A1 (en) 2016-12-15
US20130197209A1 (en) 2013-08-01
GB0405884D0 (en) 2004-04-21
US20200399692A1 (en) 2020-12-24
JP5748805B2 (ja) 2015-07-15
US20200017908A1 (en) 2020-01-16
EP1530578B1 (en) 2013-03-13
ES2550513T3 (es) 2015-11-10
EP2607369A1 (en) 2013-06-26
SI3363809T1 (sl) 2020-08-31
US20090325172A1 (en) 2009-12-31
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EP3795577A1 (en) 2021-03-24

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