RU2014144498A - Композиции для борьбы с клещами варроа у пчел - Google Patents
Композиции для борьбы с клещами варроа у пчел Download PDFInfo
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- 239000000203 mixture Substances 0.000 title claims abstract 22
- 241000895647 Varroa Species 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 38
- 108020004707 nucleic acids Proteins 0.000 claims abstract 25
- 102000039446 nucleic acids Human genes 0.000 claims abstract 25
- 239000003795 chemical substances by application Substances 0.000 claims abstract 22
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 12
- 238000000034 method Methods 0.000 claims abstract 10
- 239000007788 liquid Substances 0.000 claims abstract 5
- 102000004169 proteins and genes Human genes 0.000 claims abstract 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract 4
- 241001558516 Varroa destructor Species 0.000 claims abstract 3
- 208000015181 infectious disease Diseases 0.000 claims abstract 3
- 239000007787 solid Substances 0.000 claims abstract 3
- 244000068988 Glycine max Species 0.000 claims abstract 2
- 235000010469 Glycine max Nutrition 0.000 claims abstract 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract 2
- 229930006000 Sucrose Natural products 0.000 claims abstract 2
- 240000008042 Zea mays Species 0.000 claims abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 2
- 235000005822 corn Nutrition 0.000 claims abstract 2
- 239000005720 sucrose Substances 0.000 claims abstract 2
- 239000006188 syrup Substances 0.000 claims abstract 2
- 235000020357 syrup Nutrition 0.000 claims abstract 2
- 241000256844 Apis mellifera Species 0.000 claims 18
- 230000006378 damage Effects 0.000 claims 3
- 208000011580 syndromic disease Diseases 0.000 claims 3
- 241000256837 Apidae Species 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 238000011321 prophylaxis Methods 0.000 claims 1
- 238000011282 treatment Methods 0.000 claims 1
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- A23K20/10—Organic substances
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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Abstract
1. Выделенное нуклеиновокислотное средство, содержащее последовательность нуклеиновой кислоты, выбранную из группы, состоящей из SEQ ID NO. 93-105 и 106.2. Конструкт нуклеиновой кислоты, содержащий последовательность нуклеиновой кислоты, кодирующую выделенное нуклеиновокислотное средство по п. 1.3. Проглатываемая пчелой композиция, содержащая по меньшей мере одно нуклеиновокислотное средство по п. 1.4. Проглатываемая пчелой композиция по п. 3, содержащая по меньшей мере пять нуклеиновокислотных средств, причем каждое из нуклеиновокислотных средств характеризуется различной последовательностью нуклеиновой кислоты, выбранной из группы, состоящей из SEQ ID NO: 93-105 и 106.5. Проглатываемая пчелой композиция по п. 3, содержащая по меньшей мере одно из нуклеиновокислотных средств, характеризующееся последовательностью нуклеиновой кислоты, представленной в SEQ ID NO: 93, 96, 100, 104 и 106.6. Проглатываемая пчелой композиция по п. 3, содержащая каждое из нуклеиновокислотных средств, характеризующееся последовательностью нуклеиновой кислоты, представленной в SEQ ID NO: 93, 96, 100, 104 и 106.7. Проглатываемая пчелой композиция по п. 3, находящаяся в форме, выбранной из группы, состоящей из твердой формы и жидкой формы.8. Проглатываемая пчелой композиция по п. 3, дополнительно содержащая белок.9. Проглатываемая пчелой композиция по п. 8, в которой указанный белок находится в форме пыльцы и/или соевых лепешек.10. Проглатываемая пчелой композиция по п. 7, в которой указанная жидкая форма выбрана из группы, состоящей из раствора сахарозы и раствора кукурузной патоки.11. Способ профилактики или лечения заражения улья клещом Varroa destructor, включающий введение пчеле из указанного улья эффективного количества по меньшей мере одного нуклеиновокислотн
Claims (19)
1. Выделенное нуклеиновокислотное средство, содержащее последовательность нуклеиновой кислоты, выбранную из группы, состоящей из SEQ ID NO. 93-105 и 106.
2. Конструкт нуклеиновой кислоты, содержащий последовательность нуклеиновой кислоты, кодирующую выделенное нуклеиновокислотное средство по п. 1.
3. Проглатываемая пчелой композиция, содержащая по меньшей мере одно нуклеиновокислотное средство по п. 1.
4. Проглатываемая пчелой композиция по п. 3, содержащая по меньшей мере пять нуклеиновокислотных средств, причем каждое из нуклеиновокислотных средств характеризуется различной последовательностью нуклеиновой кислоты, выбранной из группы, состоящей из SEQ ID NO: 93-105 и 106.
5. Проглатываемая пчелой композиция по п. 3, содержащая по меньшей мере одно из нуклеиновокислотных средств, характеризующееся последовательностью нуклеиновой кислоты, представленной в SEQ ID NO: 93, 96, 100, 104 и 106.
6. Проглатываемая пчелой композиция по п. 3, содержащая каждое из нуклеиновокислотных средств, характеризующееся последовательностью нуклеиновой кислоты, представленной в SEQ ID NO: 93, 96, 100, 104 и 106.
7. Проглатываемая пчелой композиция по п. 3, находящаяся в форме, выбранной из группы, состоящей из твердой формы и жидкой формы.
8. Проглатываемая пчелой композиция по п. 3, дополнительно содержащая белок.
9. Проглатываемая пчелой композиция по п. 8, в которой указанный белок находится в форме пыльцы и/или соевых лепешек.
10. Проглатываемая пчелой композиция по п. 7, в которой указанная жидкая форма выбрана из группы, состоящей из раствора сахарозы и раствора кукурузной патоки.
11. Способ профилактики или лечения заражения улья клещом Varroa destructor, включающий введение пчеле из указанного улья эффективного количества по меньшей мере одного нуклеиновокислотного средства, характеризующегося последовательностью, выбранной из группы, состоящей из SEQ ID NO. 93-105 и 106, тем самым обеспечивая профилактику или лечение заражение указанного улья клещом Varroa destructor.
12. Способ по п. 11, при котором указанная медоносная пчела представляет собой пчелу из колонии, и при котором указанное введение снижает подверженность указанной колонии синдрому разрушения колоний.
13. Способ по п. 11, при котором указанное введение осуществляют путем кормления.
14. Способ по п. 13, при котором указанное нуклеиновокислотное средство включают в проглатываемой пчелой композиции, выбранной из группы, состоящей из жидкой проглатываемой пчелой композиции и твердой проглатываемой пчелой композиции.
15. Способ по п. 14, при котором проглатываемая пчелой композиция содержит по меньшей мере одно из нуклеиновокислотных средств, характеризующееся последовательностью нуклеиновой кислоты, представленной в SEQ ID NO: 93, 96, 100, 104 и 106.
16. Способ по п. 14, при котором проглатываемая пчелой композиция содержит по меньшей мере пять нуклеиновокислотных средств, причем каждое средство характеризуется различной последовательностью нуклеиновой кислоты, выбранной из группы, состоящей из SEQ ID NO: 93-106.
17. Способ по п. 11, при котором указанное нуклеиновокислотное средство вводят в виде конструкта нуклеиновой кислоты.
18. Способ снижения подверженности медоносной пчелы синдрому разрушения колоний (CCD), включающий введение медоносной пчеле эффективного количества по меньшей мере одного нуклеиновокислотного средства, выбранного из группы, состоящей из последовательностей нуклеиновых кислот, представленных в SEQ ID NO: 93-95 и 106, тем самым снижая подверженность медоносных пчел синдрому разрушения колоний (CCD).
19. Способ по п. 18, при котором указанные нуклеиновокислотные средства представлены в SEQ ID NO: 93, 96, 100, 104 и 106.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/446,557 US8962584B2 (en) | 2009-10-14 | 2012-04-13 | Compositions for controlling Varroa mites in bees |
| US13/446,557 | 2012-04-13 | ||
| PCT/IL2013/050321 WO2013153553A2 (en) | 2012-04-13 | 2013-04-11 | Compositions for controlling varroa mites in bees |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2014144498A true RU2014144498A (ru) | 2016-06-10 |
| RU2658771C2 RU2658771C2 (ru) | 2018-06-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014144498A RU2658771C2 (ru) | 2012-04-13 | 2013-04-11 | Композиции для борьбы с клещами варроа у пчел |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US8962584B2 (ru) |
| EP (1) | EP2836596A2 (ru) |
| CN (1) | CN104718294A (ru) |
| AU (1) | AU2013248167A1 (ru) |
| BR (1) | BR112014025500A2 (ru) |
| CA (1) | CA2869831A1 (ru) |
| CL (1) | CL2014002751A1 (ru) |
| IL (1) | IL235023A0 (ru) |
| IN (1) | IN2014MN02141A (ru) |
| MX (1) | MX2014012364A (ru) |
| NZ (1) | NZ700791A (ru) |
| RU (1) | RU2658771C2 (ru) |
| UA (1) | UA116206C2 (ru) |
| WO (1) | WO2013153553A2 (ru) |
| ZA (1) | ZA201407343B (ru) |
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| US8097712B2 (en) | 2007-11-07 | 2012-01-17 | Beelogics Inc. | Compositions for conferring tolerance to viral disease in social insects, and the use thereof |
| WO2010128465A1 (en) | 2009-05-05 | 2010-11-11 | Beeologics, Llc | Prevention and treatment of nosema disease in bees |
| US8962584B2 (en) | 2009-10-14 | 2015-02-24 | Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. | Compositions for controlling Varroa mites in bees |
| US20130047297A1 (en) | 2010-03-08 | 2013-02-21 | Robert D. Sammons | Polynucleotide molecules for gene regulation in plants |
| WO2013040033A1 (en) | 2011-09-13 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for weed control |
| US10829828B2 (en) | 2011-09-13 | 2020-11-10 | Monsanto Technology Llc | Methods and compositions for weed control |
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| MX362812B (es) | 2011-09-13 | 2019-02-13 | Monsanto Technology Llc | Metodos y composiciones para el control de malezas. |
| US10806146B2 (en) | 2011-09-13 | 2020-10-20 | Monsanto Technology Llc | Methods and compositions for weed control |
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| CN104619843B (zh) | 2012-05-24 | 2020-03-06 | A.B.种子有限公司 | 用于使基因表达沉默的组合物和方法 |
| US10683505B2 (en) | 2013-01-01 | 2020-06-16 | Monsanto Technology Llc | Methods of introducing dsRNA to plant seeds for modulating gene expression |
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| MX2015012334A (es) | 2013-03-13 | 2016-02-05 | Monsanto Technology Llc | Metodos y composiciones para el control de malezas. |
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| GB201311840D0 (en) * | 2013-07-02 | 2013-08-14 | Univ Aberdeen | Control of varroa mite infestation |
| CA2918387C (en) | 2013-07-19 | 2021-11-02 | Monsanto Technology Llc | Compositions and methods for controlling leptinotarsa |
| US9850496B2 (en) | 2013-07-19 | 2017-12-26 | Monsanto Technology Llc | Compositions and methods for controlling Leptinotarsa |
| US20150123801A1 (en) * | 2013-11-01 | 2015-05-07 | Eltopia Communications, LLC | Monitoring the state of a beehive |
| NZ719544A (en) | 2013-11-04 | 2022-09-30 | Beeologics Inc | Compositions and methods for controlling arthropod parasite and pest infestations |
| UA119253C2 (uk) * | 2013-12-10 | 2019-05-27 | Біолоджикс, Інк. | Спосіб боротьби із вірусом у кліща varroa та у бджіл |
| AR099092A1 (es) | 2014-01-15 | 2016-06-29 | Monsanto Technology Llc | Métodos y composiciones para el control de malezas utilizando polinucleótidos epsps |
| US11091770B2 (en) | 2014-04-01 | 2021-08-17 | Monsanto Technology Llc | Compositions and methods for controlling insect pests |
| CN105085643B (zh) * | 2014-05-21 | 2018-01-19 | 广东省生物资源应用研究所 | 一种狄斯瓦螨毒性蛋白及其编码基因和应用 |
| AU2015280252A1 (en) | 2014-06-23 | 2017-01-12 | Monsanto Technology Llc | Compositions and methods for regulating gene expression via RNA interference |
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-
2012
- 2012-04-13 US US13/446,557 patent/US8962584B2/en active Active
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2013
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- 2013-04-11 RU RU2014144498A patent/RU2658771C2/ru active
- 2013-04-11 IN IN2141MUN2014 patent/IN2014MN02141A/en unknown
- 2013-04-11 WO PCT/IL2013/050321 patent/WO2013153553A2/en active Application Filing
- 2013-04-11 CN CN201380031114.6A patent/CN104718294A/zh active Pending
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- 2013-04-11 BR BR112014025500A patent/BR112014025500A2/pt not_active Application Discontinuation
- 2013-04-11 MX MX2014012364A patent/MX2014012364A/es unknown
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- 2013-04-11 AU AU2013248167A patent/AU2013248167A1/en not_active Abandoned
- 2013-11-04 UA UAA201412247A patent/UA116206C2/uk unknown
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| NZ700791A (en) | 2016-08-26 |
| US8962584B2 (en) | 2015-02-24 |
| IL235023A0 (en) | 2014-12-31 |
| AU2013248167A1 (en) | 2014-10-30 |
| ZA201407343B (en) | 2015-07-29 |
| US9662348B2 (en) | 2017-05-30 |
| US20120258646A1 (en) | 2012-10-11 |
| CL2014002751A1 (es) | 2015-05-08 |
| CA2869831A1 (en) | 2013-10-17 |
| US20150133532A1 (en) | 2015-05-14 |
| US10801028B2 (en) | 2020-10-13 |
| BR112014025500A2 (pt) | 2017-08-08 |
| UA116206C2 (uk) | 2018-02-26 |
| WO2013153553A2 (en) | 2013-10-17 |
| WO2013153553A3 (en) | 2013-12-12 |
| IN2014MN02141A (ru) | 2015-08-21 |
| EP2836596A2 (en) | 2015-02-18 |
| CN104718294A (zh) | 2015-06-17 |
| MX2014012364A (es) | 2015-04-17 |
| US20170233743A1 (en) | 2017-08-17 |
| RU2658771C2 (ru) | 2018-06-22 |
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