RS50741B - Proces prečišćavanja rekombinantnog hcg - Google Patents
Proces prečišćavanja rekombinantnog hcgInfo
- Publication number
- RS50741B RS50741B YUP-602/02A YUP60202A RS50741B RS 50741 B RS50741 B RS 50741B YU P60202 A YUP60202 A YU P60202A RS 50741 B RS50741 B RS 50741B
- Authority
- RS
- Serbia
- Prior art keywords
- elution
- sample
- chromatographic column
- recombinant hcg
- purification
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title 1
- 238000010828 elution Methods 0.000 abstract 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 230000001419 dependent effect Effects 0.000 abstract 1
- 238000002270 exclusion chromatography Methods 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 238000004007 reversed phase HPLC Methods 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Diabetes (AREA)
- Gynecology & Obstetrics (AREA)
- Pregnancy & Childbirth (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Detergent Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Postupak za pripremu rekombinantnog hCG, naznačen time, koji ima specifičnu bioaktivnost u rasponu od 23000 - 28000 IJ/mg iz uzorka koji obuhvata sledeće korake:(a) elucija uzorka kroz hromatografsku kolonu od silicijum dioksida(b) elucija uzorka kroz hromatografsku kolonu za jonsku izmenu(c) elucija kroz drugu hromatografsku kolonu za jonsku izmenu(d) elucija uzorka kroz HPLC kolonu reverzne faze (e) primena eluata na hromatografiju ekskluzije,veličine.Prijava sadrži još 6 zavisnih patentnih zahteva.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00103690 | 2000-02-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
YU60202A YU60202A (sh) | 2005-09-19 |
RS50741B true RS50741B (sr) | 2010-08-31 |
Family
ID=8167925
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
YUP-602/02A RS50741B (sr) | 2000-02-22 | 2001-01-22 | Proces prečišćavanja rekombinantnog hcg |
Country Status (32)
Country | Link |
---|---|
US (2) | US7297777B2 (sr) |
EP (2) | EP1257564B1 (sr) |
JP (1) | JP4588960B2 (sr) |
KR (1) | KR100806911B1 (sr) |
CN (1) | CN100482679C (sr) |
AT (1) | ATE406378T1 (sr) |
AU (2) | AU783320B2 (sr) |
BG (1) | BG65807B1 (sr) |
BR (1) | BRPI0108556B8 (sr) |
CA (1) | CA2399020A1 (sr) |
CY (1) | CY1108460T1 (sr) |
CZ (1) | CZ303144B6 (sr) |
DE (1) | DE60135541D1 (sr) |
DK (1) | DK1257564T3 (sr) |
EA (1) | EA006605B1 (sr) |
EE (1) | EE05644B1 (sr) |
ES (1) | ES2307585T3 (sr) |
HK (1) | HK1052016A1 (sr) |
HR (1) | HRP20020650B1 (sr) |
HU (2) | HU1300063D0 (sr) |
IL (2) | IL151308A0 (sr) |
ME (1) | ME00296B (sr) |
MX (1) | MXPA02007871A (sr) |
NO (1) | NO328694B1 (sr) |
PL (1) | PL205149B1 (sr) |
PT (1) | PT1257564E (sr) |
RS (1) | RS50741B (sr) |
SI (1) | SI1257564T1 (sr) |
SK (1) | SK287146B6 (sr) |
UA (2) | UA86938C2 (sr) |
WO (1) | WO2001062773A1 (sr) |
ZA (1) | ZA200206109B (sr) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1257564T3 (da) * | 2000-02-22 | 2008-10-06 | Serono Lab | Fremgangsmåde til oprensning af rekombinant HCG med en specifik bioaktivitet |
WO2006041945A2 (en) * | 2004-10-04 | 2006-04-20 | Novetide, Ltd. | A counterion exchange process for peptides |
CN1958603B (zh) * | 2005-11-04 | 2010-05-05 | 上海天伟生物制药有限公司 | 一种人绒毛膜促性腺素的纯化方法 |
EA200802426A1 (ru) * | 2006-07-11 | 2009-04-28 | Коваль, Антон Александрович | Применение хорионического или лютеинизирующего гормона для профилактики или лечения возрастного гипогонадизма |
CN101397339B (zh) * | 2008-09-24 | 2012-07-25 | 上海天伟生物制药有限公司 | 糖蛋白中去除/灭活病毒的方法 |
TWI532495B (zh) | 2009-10-05 | 2016-05-11 | 菲瑞茵國際中心股份有限公司 | 藥學製劑 |
CN101792481B (zh) * | 2010-03-12 | 2012-05-30 | 丽珠医药集团股份有限公司 | 一种绒毛膜促性腺激素的纯化方法 |
WO2012120535A2 (en) * | 2011-02-03 | 2012-09-13 | Sanzyme Limited | A composition comprising highly purified chorionic gonadotropin, it's formulation and uses of the same |
CA3163525A1 (en) | 2011-03-31 | 2012-10-04 | Ferring Bv. | Pharmaceutical preparation |
WO2013102921A2 (en) * | 2011-11-17 | 2013-07-11 | Sanzyme Limited | Hcg - newer treatment modality for type 2 diabetes mellitus (t2dm) |
CN103288950A (zh) * | 2012-12-28 | 2013-09-11 | 青岛九龙生物医药有限公司 | 改进柱分离工序洗涤液和洗脱液配比去除杂质的方法 |
CN104101656A (zh) * | 2013-04-01 | 2014-10-15 | 上海天伟生物制药有限公司 | 一种促性腺激素的纯度测定方法 |
US20150065426A1 (en) * | 2013-08-27 | 2015-03-05 | Professional Compounding Centers Of America | Testosterone Booster Transdermal Compositions |
CN105968185A (zh) * | 2016-07-20 | 2016-09-28 | 宁波人健药业集团股份有限公司 | 一种绒促性素纯化方法 |
CN111233999B (zh) * | 2020-03-21 | 2024-02-23 | 上海浦东明炎生物技术有限公司 | 一种从人尿中提取人绒毛膜促性腺激素的方法 |
CN113398251B (zh) * | 2021-07-30 | 2022-08-05 | 宁波人健药业集团股份有限公司 | 一种重组人绒毛膜促性腺激素冻干粉针剂及其制备方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0461200T3 (da) * | 1989-02-21 | 1997-03-10 | Univ Washington | Modificerede former af reproduktionshormoner |
IT1250075B (it) * | 1991-12-18 | 1995-03-30 | Serono Cesare Ist Ricerca | Composizioni farmaceutiche contenenti gonadotropine. |
IT1254370B (it) * | 1992-05-22 | 1995-09-14 | Sorin Biomedica Spa | Procedimento per la purificazione di proteine da sistemi cellulari. |
WO1996029095A1 (en) | 1995-03-21 | 1996-09-26 | Applied Research Systems Ars Holding N.V. | Hcg liquid formulations |
CN1261579C (zh) * | 1996-02-20 | 2006-06-28 | 应用研究系统Ars控股公司 | 形成异源二聚体的杂种蛋白质 |
IT1287138B1 (it) * | 1996-11-07 | 1998-08-04 | Ibsa Inst Biochimique Sa | Procedimento per la separazione e purificazione di fsh e lh |
WO1998056806A1 (en) | 1997-06-12 | 1998-12-17 | The Regents Of The University Of California | A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP |
DK0998486T4 (da) * | 1997-06-13 | 2012-05-14 | Genentech Inc | Proteinoprensning ved kromatografi efterfulgt af filtrering på en ladet fase |
US6583109B1 (en) * | 1997-06-24 | 2003-06-24 | Robert C. Gallo | Therapeutic polypeptides from β-hCG and derivatives |
DK1257564T3 (da) * | 2000-02-22 | 2008-10-06 | Serono Lab | Fremgangsmåde til oprensning af rekombinant HCG med en specifik bioaktivitet |
-
2001
- 2001-01-22 DK DK01901188T patent/DK1257564T3/da active
- 2001-01-22 JP JP2001562554A patent/JP4588960B2/ja not_active Expired - Lifetime
- 2001-01-22 ME MEP-2008-389A patent/ME00296B/me unknown
- 2001-01-22 IL IL15130801A patent/IL151308A0/xx unknown
- 2001-01-22 AU AU26804/01A patent/AU783320B2/en not_active Expired
- 2001-01-22 BR BRPI0108556A patent/BRPI0108556B8/pt not_active IP Right Cessation
- 2001-01-22 WO PCT/EP2001/000665 patent/WO2001062773A1/en active IP Right Grant
- 2001-01-22 HU HUP1300063 patent/HU1300063D0/hu unknown
- 2001-01-22 AT AT01901188T patent/ATE406378T1/de active
- 2001-01-22 EP EP01901188A patent/EP1257564B1/en not_active Expired - Lifetime
- 2001-01-22 RS YUP-602/02A patent/RS50741B/sr unknown
- 2001-01-22 SI SI200130858T patent/SI1257564T1/sl unknown
- 2001-01-22 PT PT01901188T patent/PT1257564E/pt unknown
- 2001-01-22 EE EEP200200469A patent/EE05644B1/xx unknown
- 2001-01-22 CN CNB018053637A patent/CN100482679C/zh not_active Expired - Lifetime
- 2001-01-22 CA CA002399020A patent/CA2399020A1/en not_active Abandoned
- 2001-01-22 DE DE60135541T patent/DE60135541D1/de not_active Expired - Lifetime
- 2001-01-22 EA EA200200892A patent/EA006605B1/ru not_active IP Right Cessation
- 2001-01-22 PL PL357508A patent/PL205149B1/pl unknown
- 2001-01-22 UA UAA200511832A patent/UA86938C2/uk unknown
- 2001-01-22 CZ CZ20022855A patent/CZ303144B6/cs not_active IP Right Cessation
- 2001-01-22 UA UA2002086890A patent/UA78488C2/uk unknown
- 2001-01-22 HU HU0204231A patent/HU228935B1/hu unknown
- 2001-01-22 EP EP06024050A patent/EP1752466A3/en not_active Withdrawn
- 2001-01-22 SK SK1206-2002A patent/SK287146B6/sk not_active IP Right Cessation
- 2001-01-22 ES ES01901188T patent/ES2307585T3/es not_active Expired - Lifetime
- 2001-01-22 KR KR1020027010625A patent/KR100806911B1/ko active IP Right Grant
- 2001-01-22 MX MXPA02007871A patent/MXPA02007871A/es active IP Right Grant
- 2001-01-22 US US10/204,630 patent/US7297777B2/en not_active Expired - Lifetime
-
2002
- 2002-07-31 ZA ZA200206109A patent/ZA200206109B/en unknown
- 2002-08-01 HR HR20020650A patent/HRP20020650B1/xx not_active IP Right Cessation
- 2002-08-14 BG BG107000A patent/BG65807B1/bg unknown
- 2002-08-18 IL IL151308A patent/IL151308A/en active IP Right Grant
- 2002-08-21 NO NO20023985A patent/NO328694B1/no not_active IP Right Cessation
-
2003
- 2003-06-17 HK HK03104314.8A patent/HK1052016A1/xx unknown
-
2006
- 2006-01-06 AU AU2006200059A patent/AU2006200059A1/en not_active Abandoned
-
2007
- 2007-08-28 US US11/846,118 patent/US20070299001A1/en not_active Abandoned
-
2008
- 2008-10-27 CY CY20081101218T patent/CY1108460T1/el unknown
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