CN1404485A - 人绒毛膜促性腺激素的纯化方法及由这种方法纯化的重组人绒毛膜促性腺激素 - Google Patents
人绒毛膜促性腺激素的纯化方法及由这种方法纯化的重组人绒毛膜促性腺激素 Download PDFInfo
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- CN1404485A CN1404485A CN01805363A CN01805363A CN1404485A CN 1404485 A CN1404485 A CN 1404485A CN 01805363 A CN01805363 A CN 01805363A CN 01805363 A CN01805363 A CN 01805363A CN 1404485 A CN1404485 A CN 1404485A
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- hcg
- wash
- exchange chromatography
- ion
- agarose
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Diabetes (AREA)
- Gynecology & Obstetrics (AREA)
- Pregnancy & Childbirth (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Compounds Of Unknown Constitution (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Detergent Compositions (AREA)
Abstract
Description
来自生物反应器的培养基| | |
步骤1 | C4硅胶色谱法(洗脱液含有r-hCG)|截留分子量为10KD的超滤(存留液含有r-hCG)|浓缩的原重组人绒毛膜促性腺激素(r-hCG)收集液| |
步骤2 | 二乙氨基乙烷(DEAE)琼脂糖FF(游离馏分含有r-hCG)| |
步骤3 | 羧甲基(CM)-琼脂糖FF(洗脱液含有r-hCG)| |
步骤4 | 硅胶C18反相高效液相色谱法(洗脱液含有r-hCG)|超滤(10KD) |
步骤5 | SEPHACRYL S-200 HR粒度排斥色谱法(洗脱液含有r-hCG)|r-hCG本体溶液 |
r-Hcg批料 | 比活度[IU/mg] |
BCEA 9901 | 24427 |
BCEA 9902 | 26868 |
BCEA 9903 | 25636 |
BCEA 9904 | 27152 |
BCEA 9905 | 23729 |
成分 | 单位 | |
重组人绒毛膜促性腺激素(r-hCG) | IU/ml | 10000 |
蔗糖 | mg/ml | 102.6 |
磷酸 | mg/ml | 0.98 |
氢氧化钠 | 适量,至溶液的pH值为7.0 |
成分 | 单位 | |
重组人绒毛膜促性腺激素(r-hCG) | IU/ml | 10000 |
蔗糖 | mg/ml | 54.6 |
磷酸 | mg/ml | 0.98 |
氢氧化钠 | 适量,至溶液的pH值为7.0 |
成分 | 单位 | |
重组人绒毛膜促性腺激素(r-hCG) | IU/ml | 5000 |
蔗糖 | mg/ml | 30 |
磷酸 | mg/ml | 0.98 |
氢氧化钠 | 适量,至溶液的pH值为7.0 |
Claims (15)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00103690.4 | 2000-02-22 | ||
EP00103690 | 2000-02-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1404485A true CN1404485A (zh) | 2003-03-19 |
CN100482679C CN100482679C (zh) | 2009-04-29 |
Family
ID=8167925
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018053637A Expired - Lifetime CN100482679C (zh) | 2000-02-22 | 2001-01-22 | 人绒毛膜促性腺激素的纯化方法 |
Country Status (32)
Country | Link |
---|---|
US (2) | US7297777B2 (zh) |
EP (2) | EP1257564B1 (zh) |
JP (1) | JP4588960B2 (zh) |
KR (1) | KR100806911B1 (zh) |
CN (1) | CN100482679C (zh) |
AT (1) | ATE406378T1 (zh) |
AU (2) | AU783320B2 (zh) |
BG (1) | BG65807B1 (zh) |
BR (1) | BRPI0108556B8 (zh) |
CA (1) | CA2399020A1 (zh) |
CY (1) | CY1108460T1 (zh) |
CZ (1) | CZ303144B6 (zh) |
DE (1) | DE60135541D1 (zh) |
DK (1) | DK1257564T3 (zh) |
EA (1) | EA006605B1 (zh) |
EE (1) | EE05644B1 (zh) |
ES (1) | ES2307585T3 (zh) |
HK (1) | HK1052016A1 (zh) |
HR (1) | HRP20020650B1 (zh) |
HU (2) | HU1300063D0 (zh) |
IL (2) | IL151308A0 (zh) |
ME (1) | ME00296B (zh) |
MX (1) | MXPA02007871A (zh) |
NO (1) | NO328694B1 (zh) |
PL (1) | PL205149B1 (zh) |
PT (1) | PT1257564E (zh) |
RS (1) | RS50741B (zh) |
SI (1) | SI1257564T1 (zh) |
SK (1) | SK287146B6 (zh) |
UA (2) | UA78488C2 (zh) |
WO (1) | WO2001062773A1 (zh) |
ZA (1) | ZA200206109B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010034198A1 (zh) * | 2008-09-24 | 2010-04-01 | 上海天伟生物制药有限公司 | 糖蛋白中去除/灭活病毒的方法 |
CN1958603B (zh) * | 2005-11-04 | 2010-05-05 | 上海天伟生物制药有限公司 | 一种人绒毛膜促性腺素的纯化方法 |
CN105968185A (zh) * | 2016-07-20 | 2016-09-28 | 宁波人健药业集团股份有限公司 | 一种绒促性素纯化方法 |
CN111233999A (zh) * | 2020-03-21 | 2020-06-05 | 上海浦东明炎生物技术有限公司 | 一种从人尿中提取人绒毛膜促性腺激素的方法 |
CN113398251A (zh) * | 2021-07-30 | 2021-09-17 | 宁波人健药业集团股份有限公司 | 一种重组人绒毛膜促性腺激素冻干粉针剂及其制备方法 |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100482679C (zh) * | 2000-02-22 | 2009-04-29 | 雪兰诺实验室有限公司 | 人绒毛膜促性腺激素的纯化方法 |
US20060148699A1 (en) * | 2004-10-04 | 2006-07-06 | Avi Tovi | Counterion exchange process for peptides |
EA200802426A1 (ru) * | 2006-07-11 | 2009-04-28 | Коваль, Антон Александрович | Применение хорионического или лютеинизирующего гормона для профилактики или лечения возрастного гипогонадизма |
TWI532495B (zh) * | 2009-10-05 | 2016-05-11 | 菲瑞茵國際中心股份有限公司 | 藥學製劑 |
CN101792481B (zh) * | 2010-03-12 | 2012-05-30 | 丽珠医药集团股份有限公司 | 一种绒毛膜促性腺激素的纯化方法 |
WO2012120535A2 (en) * | 2011-02-03 | 2012-09-13 | Sanzyme Limited | A composition comprising highly purified chorionic gonadotropin, it's formulation and uses of the same |
JP6087338B2 (ja) | 2011-03-31 | 2017-03-01 | フェリング ベスローテン フェンノートシャップ | 医薬製剤 |
WO2013102921A2 (en) * | 2011-11-17 | 2013-07-11 | Sanzyme Limited | Hcg - newer treatment modality for type 2 diabetes mellitus (t2dm) |
CN103288950A (zh) * | 2012-12-28 | 2013-09-11 | 青岛九龙生物医药有限公司 | 改进柱分离工序洗涤液和洗脱液配比去除杂质的方法 |
CN104101656A (zh) * | 2013-04-01 | 2014-10-15 | 上海天伟生物制药有限公司 | 一种促性腺激素的纯度测定方法 |
US20150065426A1 (en) * | 2013-08-27 | 2015-03-05 | Professional Compounding Centers Of America | Testosterone Booster Transdermal Compositions |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU648020B2 (en) * | 1989-02-21 | 1994-04-14 | Washington University | Modified forms of reproductive hormones |
IT1250075B (it) * | 1991-12-18 | 1995-03-30 | Serono Cesare Ist Ricerca | Composizioni farmaceutiche contenenti gonadotropine. |
IT1254370B (it) * | 1992-05-22 | 1995-09-14 | Sorin Biomedica Spa | Procedimento per la purificazione di proteine da sistemi cellulari. |
KR100377967B1 (ko) | 1995-03-21 | 2003-06-12 | 어플라이드 리서치 시스템스 에이알에스 홀딩 엔.브이. | Hcg액상조성물 |
EA002474B1 (ru) * | 1996-02-20 | 2002-06-27 | Апплайд Резеч Системз Арс Холдинг Н.В. | Гибридный белок, образующий гетеродимеры, способ его получения и использования |
IT1287138B1 (it) * | 1996-11-07 | 1998-08-04 | Ibsa Inst Biochimique Sa | Procedimento per la separazione e purificazione di fsh e lh |
WO1998056806A1 (en) | 1997-06-12 | 1998-12-17 | The Regents Of The University Of California | A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP |
JP2001510483A (ja) * | 1997-06-13 | 2001-07-31 | ジェネンテク,インコーポレイテッド | タンパク質の回収 |
US6583109B1 (en) * | 1997-06-24 | 2003-06-24 | Robert C. Gallo | Therapeutic polypeptides from β-hCG and derivatives |
CN100482679C (zh) * | 2000-02-22 | 2009-04-29 | 雪兰诺实验室有限公司 | 人绒毛膜促性腺激素的纯化方法 |
-
2001
- 2001-01-22 CN CNB018053637A patent/CN100482679C/zh not_active Expired - Lifetime
- 2001-01-22 BR BRPI0108556A patent/BRPI0108556B8/pt not_active IP Right Cessation
- 2001-01-22 EA EA200200892A patent/EA006605B1/ru not_active IP Right Cessation
- 2001-01-22 UA UA2002086890A patent/UA78488C2/uk unknown
- 2001-01-22 JP JP2001562554A patent/JP4588960B2/ja not_active Expired - Lifetime
- 2001-01-22 MX MXPA02007871A patent/MXPA02007871A/es active IP Right Grant
- 2001-01-22 EP EP01901188A patent/EP1257564B1/en not_active Expired - Lifetime
- 2001-01-22 DE DE60135541T patent/DE60135541D1/de not_active Expired - Lifetime
- 2001-01-22 KR KR1020027010625A patent/KR100806911B1/ko active IP Right Grant
- 2001-01-22 HU HUP1300063 patent/HU1300063D0/hu unknown
- 2001-01-22 ME MEP-2008-389A patent/ME00296B/me unknown
- 2001-01-22 AT AT01901188T patent/ATE406378T1/de active
- 2001-01-22 DK DK01901188T patent/DK1257564T3/da active
- 2001-01-22 SK SK1206-2002A patent/SK287146B6/sk not_active IP Right Cessation
- 2001-01-22 AU AU26804/01A patent/AU783320B2/en not_active Expired
- 2001-01-22 ES ES01901188T patent/ES2307585T3/es not_active Expired - Lifetime
- 2001-01-22 IL IL15130801A patent/IL151308A0/xx unknown
- 2001-01-22 CZ CZ20022855A patent/CZ303144B6/cs not_active IP Right Cessation
- 2001-01-22 CA CA002399020A patent/CA2399020A1/en not_active Abandoned
- 2001-01-22 EE EEP200200469A patent/EE05644B1/xx unknown
- 2001-01-22 PT PT01901188T patent/PT1257564E/pt unknown
- 2001-01-22 HU HU0204231A patent/HU228935B1/hu unknown
- 2001-01-22 SI SI200130858T patent/SI1257564T1/sl unknown
- 2001-01-22 RS YUP-602/02A patent/RS50741B/sr unknown
- 2001-01-22 US US10/204,630 patent/US7297777B2/en not_active Expired - Lifetime
- 2001-01-22 WO PCT/EP2001/000665 patent/WO2001062773A1/en active IP Right Grant
- 2001-01-22 UA UAA200511832A patent/UA86938C2/uk unknown
- 2001-01-22 EP EP06024050A patent/EP1752466A3/en not_active Withdrawn
- 2001-01-22 PL PL357508A patent/PL205149B1/pl unknown
-
2002
- 2002-07-31 ZA ZA200206109A patent/ZA200206109B/en unknown
- 2002-08-01 HR HR20020650A patent/HRP20020650B1/xx not_active IP Right Cessation
- 2002-08-14 BG BG107000A patent/BG65807B1/bg unknown
- 2002-08-18 IL IL151308A patent/IL151308A/en active IP Right Grant
- 2002-08-21 NO NO20023985A patent/NO328694B1/no not_active IP Right Cessation
-
2003
- 2003-06-17 HK HK03104314.8A patent/HK1052016A1/xx unknown
-
2006
- 2006-01-06 AU AU2006200059A patent/AU2006200059A1/en not_active Abandoned
-
2007
- 2007-08-28 US US11/846,118 patent/US20070299001A1/en not_active Abandoned
-
2008
- 2008-10-27 CY CY20081101218T patent/CY1108460T1/el unknown
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1958603B (zh) * | 2005-11-04 | 2010-05-05 | 上海天伟生物制药有限公司 | 一种人绒毛膜促性腺素的纯化方法 |
WO2010034198A1 (zh) * | 2008-09-24 | 2010-04-01 | 上海天伟生物制药有限公司 | 糖蛋白中去除/灭活病毒的方法 |
CN105968185A (zh) * | 2016-07-20 | 2016-09-28 | 宁波人健药业集团股份有限公司 | 一种绒促性素纯化方法 |
CN111233999A (zh) * | 2020-03-21 | 2020-06-05 | 上海浦东明炎生物技术有限公司 | 一种从人尿中提取人绒毛膜促性腺激素的方法 |
CN111233999B (zh) * | 2020-03-21 | 2024-02-23 | 上海浦东明炎生物技术有限公司 | 一种从人尿中提取人绒毛膜促性腺激素的方法 |
CN113398251A (zh) * | 2021-07-30 | 2021-09-17 | 宁波人健药业集团股份有限公司 | 一种重组人绒毛膜促性腺激素冻干粉针剂及其制备方法 |
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