BR0108556A - Processo de purificação de hcg e hcg recombinante purificada através desse método - Google Patents

Processo de purificação de hcg e hcg recombinante purificada através desse método

Info

Publication number
BR0108556A
BR0108556A BR0108556-5A BR0108556A BR0108556A BR 0108556 A BR0108556 A BR 0108556A BR 0108556 A BR0108556 A BR 0108556A BR 0108556 A BR0108556 A BR 0108556A
Authority
BR
Brazil
Prior art keywords
hcg
recombinant
purification process
ion exchange
purifying
Prior art date
Application number
BR0108556-5A
Other languages
English (en)
Other versions
BRPI0108556B8 (pt
BRPI0108556B1 (pt
Inventor
Gianfranco Paradisi
Mara Rossi
Laura Scaglia
Original Assignee
Applied Research Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of BR0108556A publication Critical patent/BR0108556A/pt
Publication of BRPI0108556B1 publication Critical patent/BRPI0108556B1/pt
Publication of BRPI0108556B8 publication Critical patent/BRPI0108556B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Diabetes (AREA)
  • Pregnancy & Childbirth (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Detergent Compositions (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

"PROCESSO DE PURIFICAçãO DE HCG E HCG RECOMBINANTE PURIFICADA ATRAVéS DESSE MéTODO". Esta invenção refere-se a um processo para a purificação de Gonadotropina Coriónica humana recombinante (hCG) a partir de uma amostra de hCG recombinante bruta no sobrenadante de células CHO, método este que compreende o uso combinado de cromatografia de troca iónica e HPLC de fase inversa. A cromatografia de troca iónica é realizada duas vezes e o uso final de uma cromatografia de exclusão de tamanho permite a purificação de quaisquer traços residuais de contaminantes. A bioatividade específica da hCG altamente purificada obtida a partir do processo é particularmente alta, equivalendo a cerca de 25.000 IU/mg.
BRPI0108556A 2000-02-22 2001-01-22 processo para purificação de gonadotropina coriônica humana recombinante (hcg). BRPI0108556B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00103690 2000-02-22
PCT/EP2001/000665 WO2001062773A1 (en) 2000-02-22 2001-01-22 PROCESS OF PURIFICATION OF hCG AND RECOMBINANT hCG PURIFIED BY THAT METHOD

Publications (3)

Publication Number Publication Date
BR0108556A true BR0108556A (pt) 2003-01-28
BRPI0108556B1 BRPI0108556B1 (pt) 2015-04-14
BRPI0108556B8 BRPI0108556B8 (pt) 2021-05-25

Family

ID=8167925

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0108556A BRPI0108556B8 (pt) 2000-02-22 2001-01-22 processo para purificação de gonadotropina coriônica humana recombinante (hcg).

Country Status (32)

Country Link
US (2) US7297777B2 (pt)
EP (2) EP1752466A3 (pt)
JP (1) JP4588960B2 (pt)
KR (1) KR100806911B1 (pt)
CN (1) CN100482679C (pt)
AT (1) ATE406378T1 (pt)
AU (2) AU783320B2 (pt)
BG (1) BG65807B1 (pt)
BR (1) BRPI0108556B8 (pt)
CA (1) CA2399020A1 (pt)
CY (1) CY1108460T1 (pt)
CZ (1) CZ303144B6 (pt)
DE (1) DE60135541D1 (pt)
DK (1) DK1257564T3 (pt)
EA (1) EA006605B1 (pt)
EE (1) EE05644B1 (pt)
ES (1) ES2307585T3 (pt)
HK (1) HK1052016A1 (pt)
HR (1) HRP20020650B1 (pt)
HU (2) HU1300063D0 (pt)
IL (2) IL151308A0 (pt)
ME (1) ME00296B (pt)
MX (1) MXPA02007871A (pt)
NO (1) NO328694B1 (pt)
PL (1) PL205149B1 (pt)
PT (1) PT1257564E (pt)
RS (1) RS50741B (pt)
SI (1) SI1257564T1 (pt)
SK (1) SK287146B6 (pt)
UA (2) UA78488C2 (pt)
WO (1) WO2001062773A1 (pt)
ZA (1) ZA200206109B (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1257564T1 (sl) * 2000-02-22 2008-12-31 Serono Lab Postopek za äśiĺ äśenje rekombinantnega hcg s specifiäśno bioaktivnostjo
DK1709065T3 (da) * 2004-10-04 2010-08-23 Novetide Ltd Modion-bytningsfremgangsmåde til peptider
CN1958603B (zh) * 2005-11-04 2010-05-05 上海天伟生物制药有限公司 一种人绒毛膜促性腺素的纯化方法
WO2008007993A1 (fr) * 2006-07-11 2008-01-17 Koval, Anton Alexandrovich Utilisation d'une hormone chorionique ou lutéinisante destinée à la prévention ou au traitement de l'hypogonadisme lié à l'âge
CN101397339B (zh) * 2008-09-24 2012-07-25 上海天伟生物制药有限公司 糖蛋白中去除/灭活病毒的方法
TWI532495B (zh) 2009-10-05 2016-05-11 菲瑞茵國際中心股份有限公司 藥學製劑
CN101792481B (zh) * 2010-03-12 2012-05-30 丽珠医药集团股份有限公司 一种绒毛膜促性腺激素的纯化方法
WO2012120535A2 (en) * 2011-02-03 2012-09-13 Sanzyme Limited A composition comprising highly purified chorionic gonadotropin, it's formulation and uses of the same
US9757469B2 (en) 2011-03-31 2017-09-12 Ferring B.V. Pharmaceutical preparation
WO2013102921A2 (en) * 2011-11-17 2013-07-11 Sanzyme Limited Hcg - newer treatment modality for type 2 diabetes mellitus (t2dm)
CN103288950A (zh) * 2012-12-28 2013-09-11 青岛九龙生物医药有限公司 改进柱分离工序洗涤液和洗脱液配比去除杂质的方法
CN104101656A (zh) * 2013-04-01 2014-10-15 上海天伟生物制药有限公司 一种促性腺激素的纯度测定方法
US20150065426A1 (en) * 2013-08-27 2015-03-05 Professional Compounding Centers Of America Testosterone Booster Transdermal Compositions
CN105968185A (zh) * 2016-07-20 2016-09-28 宁波人健药业集团股份有限公司 一种绒促性素纯化方法
CN111233999B (zh) * 2020-03-21 2024-02-23 上海浦东明炎生物技术有限公司 一种从人尿中提取人绒毛膜促性腺激素的方法
CN113398251B (zh) * 2021-07-30 2022-08-05 宁波人健药业集团股份有限公司 一种重组人绒毛膜促性腺激素冻干粉针剂及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69029799T2 (de) * 1989-02-21 1997-05-28 Univ Washington Modifizierte formen von fortpflanzungshormonen
IT1250075B (it) * 1991-12-18 1995-03-30 Serono Cesare Ist Ricerca Composizioni farmaceutiche contenenti gonadotropine.
IT1254370B (it) 1992-05-22 1995-09-14 Sorin Biomedica Spa Procedimento per la purificazione di proteine da sistemi cellulari.
KR100377967B1 (ko) 1995-03-21 2003-06-12 어플라이드 리서치 시스템스 에이알에스 홀딩 엔.브이. Hcg액상조성물
EP0894141B1 (en) * 1996-02-20 2005-05-18 Applied Research Systems ARS Holding N.V. Hybrid proteins which form heterodimers
IT1287138B1 (it) * 1996-11-07 1998-08-04 Ibsa Inst Biochimique Sa Procedimento per la separazione e purificazione di fsh e lh
WO1998056806A1 (en) 1997-06-12 1998-12-17 The Regents Of The University Of California A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP
DE69837760T3 (de) * 1997-06-13 2012-06-21 Genentech, Inc. Proteinreinigung mittels chromatographie und darauffolgende filtration auf beladener schicht
US6583109B1 (en) * 1997-06-24 2003-06-24 Robert C. Gallo Therapeutic polypeptides from β-hCG and derivatives
SI1257564T1 (sl) * 2000-02-22 2008-12-31 Serono Lab Postopek za äśiĺ äśenje rekombinantnega hcg s specifiäśno bioaktivnostjo

Also Published As

Publication number Publication date
UA78488C2 (en) 2007-04-10
IL151308A (en) 2009-11-18
SK12062002A3 (sk) 2002-12-03
US20030104553A1 (en) 2003-06-05
EP1257564B1 (en) 2008-08-27
MEP38908A (en) 2011-02-10
YU60202A (sh) 2005-09-19
CA2399020A1 (en) 2001-08-30
EP1257564A1 (en) 2002-11-20
SK287146B6 (sk) 2010-01-07
EE05644B1 (et) 2013-04-15
BRPI0108556B8 (pt) 2021-05-25
US7297777B2 (en) 2007-11-20
US20070299001A1 (en) 2007-12-27
BRPI0108556B1 (pt) 2015-04-14
RS50741B (sr) 2010-08-31
PL205149B1 (pl) 2010-03-31
ES2307585T3 (es) 2008-12-01
SI1257564T1 (sl) 2008-12-31
EA006605B1 (ru) 2006-02-24
JP2003524015A (ja) 2003-08-12
HRP20020650A2 (en) 2004-12-31
ZA200206109B (en) 2003-07-31
KR100806911B1 (ko) 2008-02-22
DK1257564T3 (da) 2008-10-06
WO2001062773A1 (en) 2001-08-30
NO20023985D0 (no) 2002-08-21
AU783320B2 (en) 2005-10-13
JP4588960B2 (ja) 2010-12-01
HK1052016A1 (en) 2003-08-29
EE200200469A (et) 2004-04-15
EP1752466A2 (en) 2007-02-14
HRP20020650B1 (en) 2011-02-28
HUP0204231A2 (hu) 2003-03-28
PL357508A1 (en) 2004-07-26
CZ303144B6 (cs) 2012-05-02
CN100482679C (zh) 2009-04-29
CY1108460T1 (el) 2014-04-09
BG107000A (en) 2003-04-30
AU2006200059A1 (en) 2006-02-02
EA200200892A1 (ru) 2003-02-27
NO20023985L (no) 2002-08-21
BG65807B1 (bg) 2009-12-31
KR20030001359A (ko) 2003-01-06
DE60135541D1 (de) 2008-10-09
PT1257564E (pt) 2008-09-10
HU1300063D0 (hu) 2003-03-28
CN1404485A (zh) 2003-03-19
NO328694B1 (no) 2010-04-26
ME00296B (me) 2011-05-10
CZ20022855A3 (cs) 2002-11-13
UA86938C2 (uk) 2009-06-10
HU228935B1 (en) 2013-06-28
HUP0204231A3 (en) 2005-09-28
IL151308A0 (en) 2003-04-10
ATE406378T1 (de) 2008-09-15
MXPA02007871A (es) 2003-02-10
AU2680401A (en) 2001-09-03
EP1752466A3 (en) 2012-10-10

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Legal Events

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B25A Requested transfer of rights approved

Owner name: LABORATOIRES SERONO S.A. (CH)

Free format text: TRANSFERIDO DE: APPLIED RESEARCH SYSTEMS ARS HOLDING N.V.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B25D Requested change of name of applicant approved

Owner name: MERCK SERONO S.A. (CH)

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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B16C Correction of notification of the grant [chapter 16.3 patent gazette]

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