ME00296B - PROCES PREČIŠĆAVANJA hCG I REKOMBINANTNOG hCG PREČIŠĆENOG TOM METODOM - Google Patents

PROCES PREČIŠĆAVANJA hCG I REKOMBINANTNOG hCG PREČIŠĆENOG TOM METODOM

Info

Publication number
ME00296B
ME00296B MEP-2008-389A MEP38908A ME00296B ME 00296 B ME00296 B ME 00296B ME P38908 A MEP38908 A ME P38908A ME 00296 B ME00296 B ME 00296B
Authority
ME
Montenegro
Prior art keywords
hcg
purification
recombinant
ion
purified
Prior art date
Application number
MEP-2008-389A
Other languages
English (en)
French (fr)
Inventor
Gianfranco Paradisi
Mara Rossi
Laura Scaglia
Original Assignee
Applied Res Systems Ars Holding N V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Res Systems Ars Holding N V filed Critical Applied Res Systems Ars Holding N V
Publication of MEP38908A publication Critical patent/MEP38908A/xx
Publication of ME00296B publication Critical patent/ME00296B/me

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

Proces prečišćavanja rekombinantnog humanog horionskog gonadotropina (hCG) iz uzorka sirovog rekombinantnog hCG u supematantu CHO ćelija obuhvata kombinovanu upotrebu hromatografije jonske izmene i HPLC reverzne faze. Hromatografija jonske izmene je obavlja dva puta, i finalna upotreba hromatografije za isključivanje po veličini omogućuje prečišćavanje od svih rezidualnih tragova zagađivača. Specfična biološka aktivnost visoko prečišćenog hCG dobijenog ovim procesom je posebno visoka, i iznosi do 25. 000 IJ/mg.
MEP-2008-389A 2000-02-22 2001-01-22 PROCES PREČIŠĆAVANJA hCG I REKOMBINANTNOG hCG PREČIŠĆENOG TOM METODOM ME00296B (me)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00103690 2000-02-22
PCT/EP2001/000665 WO2001062773A1 (en) 2000-02-22 2001-01-22 PROCESS OF PURIFICATION OF hCG AND RECOMBINANT hCG PURIFIED BY THAT METHOD

Publications (2)

Publication Number Publication Date
MEP38908A MEP38908A (en) 2011-02-10
ME00296B true ME00296B (me) 2011-05-10

Family

ID=8167925

Family Applications (1)

Application Number Title Priority Date Filing Date
MEP-2008-389A ME00296B (me) 2000-02-22 2001-01-22 PROCES PREČIŠĆAVANJA hCG I REKOMBINANTNOG hCG PREČIŠĆENOG TOM METODOM

Country Status (32)

Country Link
US (2) US7297777B2 (me)
EP (2) EP1752466A3 (me)
JP (1) JP4588960B2 (me)
KR (1) KR100806911B1 (me)
CN (1) CN100482679C (me)
AT (1) ATE406378T1 (me)
AU (2) AU783320B2 (me)
BG (1) BG65807B1 (me)
BR (1) BRPI0108556B8 (me)
CA (1) CA2399020A1 (me)
CY (1) CY1108460T1 (me)
CZ (1) CZ303144B6 (me)
DE (1) DE60135541D1 (me)
DK (1) DK1257564T3 (me)
EA (1) EA006605B1 (me)
EE (1) EE05644B1 (me)
ES (1) ES2307585T3 (me)
HK (1) HK1052016A1 (me)
HR (1) HRP20020650B1 (me)
HU (2) HU228935B1 (me)
IL (2) IL151308A0 (me)
ME (1) ME00296B (me)
MX (1) MXPA02007871A (me)
NO (1) NO328694B1 (me)
PL (1) PL205149B1 (me)
PT (1) PT1257564E (me)
RS (1) RS50741B (me)
SI (1) SI1257564T1 (me)
SK (1) SK287146B6 (me)
UA (2) UA78488C2 (me)
WO (1) WO2001062773A1 (me)
ZA (1) ZA200206109B (me)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA006605B1 (ru) * 2000-02-22 2006-02-24 Апплайд Резеч Системз Арс Холдинг Н.В. СПОСОБ ОЧИСТКИ РЕКОМБИНАНТНОГО чХГ
CA2582083A1 (en) * 2004-10-04 2006-04-20 Novetide, Ltd. A counterion exchange process for peptides
CN1958603B (zh) * 2005-11-04 2010-05-05 上海天伟生物制药有限公司 一种人绒毛膜促性腺素的纯化方法
EA200802426A1 (ru) * 2006-07-11 2009-04-28 Коваль, Антон Александрович Применение хорионического или лютеинизирующего гормона для профилактики или лечения возрастного гипогонадизма
CN101397339B (zh) * 2008-09-24 2012-07-25 上海天伟生物制药有限公司 糖蛋白中去除/灭活病毒的方法
TWI532495B (zh) 2009-10-05 2016-05-11 菲瑞茵國際中心股份有限公司 藥學製劑
CN101792481B (zh) * 2010-03-12 2012-05-30 丽珠医药集团股份有限公司 一种绒毛膜促性腺激素的纯化方法
WO2012120535A2 (en) * 2011-02-03 2012-09-13 Sanzyme Limited A composition comprising highly purified chorionic gonadotropin, it's formulation and uses of the same
EP2691119B1 (en) 2011-03-31 2018-08-01 Ferring B.V. Pharmaceutical preparation
WO2013102921A2 (en) * 2011-11-17 2013-07-11 Sanzyme Limited Hcg - newer treatment modality for type 2 diabetes mellitus (t2dm)
CN103288950A (zh) * 2012-12-28 2013-09-11 青岛九龙生物医药有限公司 改进柱分离工序洗涤液和洗脱液配比去除杂质的方法
CN104101656A (zh) * 2013-04-01 2014-10-15 上海天伟生物制药有限公司 一种促性腺激素的纯度测定方法
US20150065426A1 (en) * 2013-08-27 2015-03-05 Professional Compounding Centers Of America Testosterone Booster Transdermal Compositions
CN105968185A (zh) * 2016-07-20 2016-09-28 宁波人健药业集团股份有限公司 一种绒促性素纯化方法
CN111233999B (zh) * 2020-03-21 2024-02-23 上海浦东明炎生物技术有限公司 一种从人尿中提取人绒毛膜促性腺激素的方法
CN113398251B (zh) * 2021-07-30 2022-08-05 宁波人健药业集团股份有限公司 一种重组人绒毛膜促性腺激素冻干粉针剂及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3045539B2 (ja) * 1989-02-21 2000-05-29 ワシントン ユニバーシティ 改変型生殖ホルモン
IT1250075B (it) * 1991-12-18 1995-03-30 Serono Cesare Ist Ricerca Composizioni farmaceutiche contenenti gonadotropine.
IT1254370B (it) * 1992-05-22 1995-09-14 Sorin Biomedica Spa Procedimento per la purificazione di proteine da sistemi cellulari.
EP0814841B1 (en) 1995-03-21 2001-12-05 Applied Research Systems ARS Holding N.V. Hcg liquid formulations
HUP9900619A3 (en) * 1996-02-20 2001-11-28 Applied Research Systems Hybrid proteins which form heterodimers
IT1287138B1 (it) * 1996-11-07 1998-08-04 Ibsa Inst Biochimique Sa Procedimento per la separazione e purificazione di fsh e lh
AU8142198A (en) 1997-06-12 1998-12-30 Regents Of The University Of California, The A transcription factor coactivator protein, p/cip
CA2289665C (en) * 1997-06-13 2005-08-09 Genentech, Inc. Protein recovery by chromatography followed by filtration upon a charged layer
US6583109B1 (en) * 1997-06-24 2003-06-24 Robert C. Gallo Therapeutic polypeptides from β-hCG and derivatives
EA006605B1 (ru) * 2000-02-22 2006-02-24 Апплайд Резеч Системз Арс Холдинг Н.В. СПОСОБ ОЧИСТКИ РЕКОМБИНАНТНОГО чХГ

Also Published As

Publication number Publication date
EE200200469A (et) 2004-04-15
NO20023985D0 (no) 2002-08-21
MEP38908A (en) 2011-02-10
RS50741B (sr) 2010-08-31
NO20023985L (no) 2002-08-21
UA86938C2 (uk) 2009-06-10
DK1257564T3 (da) 2008-10-06
CY1108460T1 (el) 2014-04-09
MXPA02007871A (es) 2003-02-10
KR20030001359A (ko) 2003-01-06
BRPI0108556B8 (pt) 2021-05-25
SK287146B6 (sk) 2010-01-07
EP1752466A2 (en) 2007-02-14
PL205149B1 (pl) 2010-03-31
WO2001062773A1 (en) 2001-08-30
YU60202A (sh) 2005-09-19
AU2680401A (en) 2001-09-03
HUP0204231A2 (hu) 2003-03-28
HU228935B1 (en) 2013-06-28
AU2006200059A1 (en) 2006-02-02
SK12062002A3 (sk) 2002-12-03
HRP20020650A2 (en) 2004-12-31
US20070299001A1 (en) 2007-12-27
CN100482679C (zh) 2009-04-29
BG65807B1 (bg) 2009-12-31
HK1052016A1 (en) 2003-08-29
DE60135541D1 (de) 2008-10-09
BRPI0108556B1 (pt) 2015-04-14
BR0108556A (pt) 2003-01-28
JP4588960B2 (ja) 2010-12-01
UA78488C2 (en) 2007-04-10
HRP20020650B1 (en) 2011-02-28
EE05644B1 (et) 2013-04-15
IL151308A0 (en) 2003-04-10
KR100806911B1 (ko) 2008-02-22
CZ303144B6 (cs) 2012-05-02
NO328694B1 (no) 2010-04-26
HUP0204231A3 (en) 2005-09-28
HU1300063D0 (hu) 2003-03-28
ES2307585T3 (es) 2008-12-01
ZA200206109B (en) 2003-07-31
US7297777B2 (en) 2007-11-20
US20030104553A1 (en) 2003-06-05
EP1752466A3 (en) 2012-10-10
PT1257564E (pt) 2008-09-10
EA006605B1 (ru) 2006-02-24
IL151308A (en) 2009-11-18
EP1257564B1 (en) 2008-08-27
EA200200892A1 (ru) 2003-02-27
JP2003524015A (ja) 2003-08-12
BG107000A (en) 2003-04-30
SI1257564T1 (sl) 2008-12-31
CZ20022855A3 (cs) 2002-11-13
EP1257564A1 (en) 2002-11-20
CA2399020A1 (en) 2001-08-30
ATE406378T1 (de) 2008-09-15
PL357508A1 (en) 2004-07-26
AU783320B2 (en) 2005-10-13
CN1404485A (zh) 2003-03-19

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