NO20006181L - Ekspresjonsvektor - Google Patents

Ekspresjonsvektor

Info

Publication number
NO20006181L
NO20006181L NO20006181A NO20006181A NO20006181L NO 20006181 L NO20006181 L NO 20006181L NO 20006181 A NO20006181 A NO 20006181A NO 20006181 A NO20006181 A NO 20006181A NO 20006181 L NO20006181 L NO 20006181L
Authority
NO
Norway
Prior art keywords
fusion protein
nucleic
sepha
discoidin
lectin
Prior art date
Application number
NO20006181A
Other languages
English (en)
Other versions
NO20006181D0 (no
Inventor
Christophe Dominique Reymond
Nicolas Joseph Fasel
Original Assignee
Rmf Dictagene Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rmf Dictagene Sa filed Critical Rmf Dictagene Sa
Publication of NO20006181D0 publication Critical patent/NO20006181D0/no
Publication of NO20006181L publication Critical patent/NO20006181L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/445—Plasmodium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/91—Fusion polypeptide containing a motif for post-translational modification containing a motif for glycosylation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Det beskrives, en ekspresjonsvektor som kan brukes med en éntrinns renseprosess. Det rekombinante protein frmstilles som et fusjonsprotein med et lektin, spesielt et diskoidin, som rensemarkør. Fusjonsproteinet kan renses på en Sepha-rose 4B-kromatografikolonne ved bruk av galaktose som elue-ringsmiddel.
NO20006181A 1998-06-16 2000-12-05 Ekspresjonsvektor NO20006181L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98202018 1998-06-16
PCT/EP1999/004157 WO1999066053A1 (en) 1998-06-16 1999-06-16 Expression vector for use in a one-step purification protocol

Publications (2)

Publication Number Publication Date
NO20006181D0 NO20006181D0 (no) 2000-12-05
NO20006181L true NO20006181L (no) 2001-02-08

Family

ID=8233820

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20006181A NO20006181L (no) 1998-06-16 2000-12-05 Ekspresjonsvektor

Country Status (16)

Country Link
US (1) US6482607B1 (no)
EP (1) EP1088083A1 (no)
JP (1) JP2002518017A (no)
KR (1) KR20010071342A (no)
CN (1) CN1305531A (no)
AU (1) AU762984B2 (no)
CA (1) CA2333602A1 (no)
CZ (1) CZ20004732A3 (no)
HU (1) HUP0102562A3 (no)
IL (1) IL140027A0 (no)
NO (1) NO20006181L (no)
NZ (1) NZ508676A (no)
PL (1) PL345091A1 (no)
RU (1) RU2241035C2 (no)
WO (1) WO1999066053A1 (no)
ZA (1) ZA200006387B (no)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1165813A2 (en) 1999-03-24 2002-01-02 The Board Of Regents, The University Of Texas System Linear and circular expression elements
US7094345B2 (en) * 2002-09-09 2006-08-22 Cytonome, Inc. Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system
JP4891581B2 (ja) * 2005-09-02 2012-03-07 ゼライス株式会社 ポリペプチドの製造方法およびキット
ES2327309B1 (es) * 2008-04-02 2010-07-26 Consejo Superior De Investigaciones Cientificas (Csic) Proteinas de fusion con un dominio lectina de tipo beta-trebol, procedimiento de obtencion y sus aplicaciones.
US9115205B2 (en) 2010-10-18 2015-08-25 The United States Of America, As Represented By The Secretary Of The Army Plasmodium falciparum circumsporozoite vaccine gene optimization for soluble protein expression
CN111057155A (zh) * 2019-12-31 2020-04-24 河南农业大学 O型fmdv vp1蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法
CN111171157A (zh) * 2019-12-31 2020-05-19 河南农业大学 A型fmdv 1d蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法
CN111116757A (zh) * 2019-12-31 2020-05-08 河南农业大学 带有半乳糖结合凝集素ew29标签的铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法
CN111057156A (zh) * 2019-12-31 2020-04-24 河南农业大学 O型fmdv衣壳蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法
EP4491629A1 (en) 2023-07-13 2025-01-15 Université de Lorraine Expression system including an engineered lectin property purification tag based on galectin-1
CN120989111A (zh) * 2025-10-17 2025-11-21 深圳市库源生物科技有限公司 一种利用HEK293T高效表达及纯化Taq DNA聚合酶的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5208149A (en) * 1983-10-20 1993-05-04 The Research Foundation Of State University Of New York Nucleic acid constructs containing stable stem and loop structures
NL9100869A (nl) 1991-05-17 1992-12-16 Univ Lausanne Dictyostelide expressievector en werkwijze voor het tot expressie brengen van een gewenst eiwit.
US6051397A (en) * 1993-11-16 2000-04-18 Max Planck Gesellschaft Zur Forderung Der Wissenschaften DNA encoding MCK-10, a novel receptor tyrosine kinase

Also Published As

Publication number Publication date
HUP0102562A2 (hu) 2001-10-28
NO20006181D0 (no) 2000-12-05
US6482607B1 (en) 2002-11-19
KR20010071342A (ko) 2001-07-28
RU2241035C2 (ru) 2004-11-27
CN1305531A (zh) 2001-07-25
WO1999066053A1 (en) 1999-12-23
AU762984B2 (en) 2003-07-10
ZA200006387B (en) 2001-06-28
JP2002518017A (ja) 2002-06-25
IL140027A0 (en) 2002-02-10
NZ508676A (en) 2002-07-26
AU4514199A (en) 2000-01-05
CA2333602A1 (en) 1999-12-23
HUP0102562A3 (en) 2005-10-28
CZ20004732A3 (cs) 2001-08-15
PL345091A1 (en) 2001-12-03
EP1088083A1 (en) 2001-04-04

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