HUP0102562A2 - Expressziós vektor egyetlen lépésből álló tisztítási eljárásban történő alkalmazásra - Google Patents
Expressziós vektor egyetlen lépésből álló tisztítási eljárásban történő alkalmazásraInfo
- Publication number
- HUP0102562A2 HUP0102562A2 HU0102562A HUP0102562A HUP0102562A2 HU P0102562 A2 HUP0102562 A2 HU P0102562A2 HU 0102562 A HU0102562 A HU 0102562A HU P0102562 A HUP0102562 A HU P0102562A HU P0102562 A2 HUP0102562 A2 HU P0102562A2
- Authority
- HU
- Hungary
- Prior art keywords
- expression vector
- step purification
- purification protocol
- polypeptides
- protocol
- Prior art date
Links
- 238000000746 purification Methods 0.000 title abstract 3
- 239000013604 expression vector Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/445—Plasmodium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/91—Fusion polypeptide containing a motif for post-translational modification containing a motif for glycosylation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A találmány tárgya polipeptidek termelésében alkalmazható tisztításieljárás, valamint az eljárásban alkalmazható expressziós vektor. Atalálmány szerinti megoldás alkalmas rekombináns úton előállítottpolipeptidek hatékony tisztítására. Ó
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98202018 | 1998-06-16 | ||
PCT/EP1999/004157 WO1999066053A1 (en) | 1998-06-16 | 1999-06-16 | Expression vector for use in a one-step purification protocol |
Publications (2)
Publication Number | Publication Date |
---|---|
HUP0102562A2 true HUP0102562A2 (hu) | 2001-10-28 |
HUP0102562A3 HUP0102562A3 (en) | 2005-10-28 |
Family
ID=8233820
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU0102562A HUP0102562A3 (en) | 1998-06-16 | 1999-06-16 | Expression vector for use in a one-step purification protocol |
Country Status (16)
Country | Link |
---|---|
US (1) | US6482607B1 (hu) |
EP (1) | EP1088083A1 (hu) |
JP (1) | JP2002518017A (hu) |
KR (1) | KR20010071342A (hu) |
CN (1) | CN1305531A (hu) |
AU (1) | AU762984B2 (hu) |
CA (1) | CA2333602A1 (hu) |
CZ (1) | CZ20004732A3 (hu) |
HU (1) | HUP0102562A3 (hu) |
IL (1) | IL140027A0 (hu) |
NO (1) | NO20006181L (hu) |
NZ (1) | NZ508676A (hu) |
PL (1) | PL345091A1 (hu) |
RU (1) | RU2241035C2 (hu) |
WO (1) | WO1999066053A1 (hu) |
ZA (1) | ZA200006387B (hu) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6410241B1 (en) | 1999-03-24 | 2002-06-25 | Board Of Regents, The University Of Texas System | Methods of screening open reading frames to determine whether they encode polypeptides with an ability to generate an immune response |
US7094345B2 (en) * | 2002-09-09 | 2006-08-22 | Cytonome, Inc. | Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system |
JP4891581B2 (ja) * | 2005-09-02 | 2012-03-07 | ゼライス株式会社 | ポリペプチドの製造方法およびキット |
ES2327309B1 (es) * | 2008-04-02 | 2010-07-26 | Consejo Superior De Investigaciones Cientificas (Csic) | Proteinas de fusion con un dominio lectina de tipo beta-trebol, procedimiento de obtencion y sus aplicaciones. |
US9115205B2 (en) | 2010-10-18 | 2015-08-25 | The United States Of America, As Represented By The Secretary Of The Army | Plasmodium falciparum circumsporozoite vaccine gene optimization for soluble protein expression |
CN111057155A (zh) * | 2019-12-31 | 2020-04-24 | 河南农业大学 | O型fmdv vp1蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法 |
CN111171157A (zh) * | 2019-12-31 | 2020-05-19 | 河南农业大学 | A型fmdv 1d蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法 |
CN111057156A (zh) * | 2019-12-31 | 2020-04-24 | 河南农业大学 | O型fmdv衣壳蛋白-铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法 |
CN111116757A (zh) * | 2019-12-31 | 2020-05-08 | 河南农业大学 | 带有半乳糖结合凝集素ew29标签的铁蛋白融合蛋白、蛋白笼纳米颗粒及其制备方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL9100869A (nl) | 1991-05-17 | 1992-12-16 | Univ Lausanne | Dictyostelide expressievector en werkwijze voor het tot expressie brengen van een gewenst eiwit. |
US6051397A (en) * | 1993-11-16 | 2000-04-18 | Max Planck Gesellschaft Zur Forderung Der Wissenschaften | DNA encoding MCK-10, a novel receptor tyrosine kinase |
-
1999
- 1999-06-15 US US09/332,902 patent/US6482607B1/en not_active Expired - Fee Related
- 1999-06-16 JP JP2000554862A patent/JP2002518017A/ja active Pending
- 1999-06-16 AU AU45141/99A patent/AU762984B2/en not_active Ceased
- 1999-06-16 HU HU0102562A patent/HUP0102562A3/hu unknown
- 1999-06-16 CA CA002333602A patent/CA2333602A1/en not_active Abandoned
- 1999-06-16 WO PCT/EP1999/004157 patent/WO1999066053A1/en not_active Application Discontinuation
- 1999-06-16 RU RU2001101528/13A patent/RU2241035C2/ru not_active IP Right Cessation
- 1999-06-16 KR KR1020007013412A patent/KR20010071342A/ko active IP Right Grant
- 1999-06-16 IL IL14002799A patent/IL140027A0/xx unknown
- 1999-06-16 EP EP99927993A patent/EP1088083A1/en not_active Withdrawn
- 1999-06-16 NZ NZ508676A patent/NZ508676A/en unknown
- 1999-06-16 PL PL99345091A patent/PL345091A1/xx unknown
- 1999-06-16 CZ CZ20004732A patent/CZ20004732A3/cs unknown
- 1999-06-16 CN CN99807293A patent/CN1305531A/zh active Pending
-
2000
- 2000-11-07 ZA ZA200006387A patent/ZA200006387B/xx unknown
- 2000-12-05 NO NO20006181A patent/NO20006181L/no not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
HUP0102562A3 (en) | 2005-10-28 |
JP2002518017A (ja) | 2002-06-25 |
CN1305531A (zh) | 2001-07-25 |
ZA200006387B (en) | 2001-06-28 |
NO20006181D0 (no) | 2000-12-05 |
CZ20004732A3 (cs) | 2001-08-15 |
NO20006181L (no) | 2001-02-08 |
NZ508676A (en) | 2002-07-26 |
AU762984B2 (en) | 2003-07-10 |
US6482607B1 (en) | 2002-11-19 |
AU4514199A (en) | 2000-01-05 |
CA2333602A1 (en) | 1999-12-23 |
RU2241035C2 (ru) | 2004-11-27 |
IL140027A0 (en) | 2002-02-10 |
KR20010071342A (ko) | 2001-07-28 |
PL345091A1 (en) | 2001-12-03 |
EP1088083A1 (en) | 2001-04-04 |
WO1999066053A1 (en) | 1999-12-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD9A | Lapse of provisional protection due to non-payment of fees |