KR102438495B1 - 단일 세포를 위한 샘플 인덱싱 - Google Patents
단일 세포를 위한 샘플 인덱싱 Download PDFInfo
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- KR102438495B1 KR102438495B1 KR1020197038794A KR20197038794A KR102438495B1 KR 102438495 B1 KR102438495 B1 KR 102438495B1 KR 1020197038794 A KR1020197038794 A KR 1020197038794A KR 20197038794 A KR20197038794 A KR 20197038794A KR 102438495 B1 KR102438495 B1 KR 102438495B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
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- C12Q2600/00—Oligonucleotides characterized by their use
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- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020227029623A KR102790050B1 (ko) | 2017-06-05 | 2018-03-27 | 단일 세포를 위한 샘플 인덱싱 |
Applications Claiming Priority (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762515285P | 2017-06-05 | 2017-06-05 | |
| US62/515,285 | 2017-06-05 | ||
| US201762532971P | 2017-07-14 | 2017-07-14 | |
| US201762532949P | 2017-07-14 | 2017-07-14 | |
| US201762532905P | 2017-07-14 | 2017-07-14 | |
| US62/532,905 | 2017-07-14 | ||
| US62/532,971 | 2017-07-14 | ||
| US62/532,949 | 2017-07-14 | ||
| US201762554425P | 2017-09-05 | 2017-09-05 | |
| US62/554,425 | 2017-09-05 | ||
| US201762578957P | 2017-10-30 | 2017-10-30 | |
| US62/578,957 | 2017-10-30 | ||
| US201862645703P | 2018-03-20 | 2018-03-20 | |
| US62/645,703 | 2018-03-20 | ||
| PCT/US2018/024602 WO2018226293A1 (en) | 2017-06-05 | 2018-03-27 | Sample indexing for single cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020227029623A Division KR102790050B1 (ko) | 2017-06-05 | 2018-03-27 | 단일 세포를 위한 샘플 인덱싱 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20200014370A KR20200014370A (ko) | 2020-02-10 |
| KR102438495B1 true KR102438495B1 (ko) | 2022-09-01 |
Family
ID=61913700
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197038794A Active KR102438495B1 (ko) | 2017-06-05 | 2018-03-27 | 단일 세포를 위한 샘플 인덱싱 |
| KR1020227029623A Active KR102790050B1 (ko) | 2017-06-05 | 2018-03-27 | 단일 세포를 위한 샘플 인덱싱 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020227029623A Active KR102790050B1 (ko) | 2017-06-05 | 2018-03-27 | 단일 세포를 위한 샘플 인덱싱 |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US10676779B2 (enExample) |
| EP (2) | EP4345172A3 (enExample) |
| JP (3) | JP7536450B2 (enExample) |
| KR (2) | KR102438495B1 (enExample) |
| CN (1) | CN110719959B (enExample) |
| AU (2) | AU2018281745B2 (enExample) |
| CA (1) | CA3059559A1 (enExample) |
| WO (1) | WO2018226293A1 (enExample) |
Families Citing this family (117)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| EP2820158B1 (en) | 2012-02-27 | 2018-01-10 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| WO2014028537A1 (en) | 2012-08-14 | 2014-02-20 | 10X Technologies, Inc. | Microcapsule compositions and methods |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2014214682B2 (en) | 2013-02-08 | 2018-07-26 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| JP6545682B2 (ja) | 2013-08-28 | 2019-07-17 | ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company | 大規模並列単一細胞分析 |
| WO2015200869A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| MX2016016902A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas. |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| MX367432B (es) | 2015-01-12 | 2019-08-08 | 10X Genomics Inc | Procesos y sistemas para la preparación de bibliotecas de secuenciación de ácido nucleico y bibliotecas preparadas con estos. |
| ES2836802T3 (es) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Códigos de barras moleculares espacialmente direccionables |
| EP3277843A2 (en) | 2015-03-30 | 2018-02-07 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| WO2017044574A1 (en) | 2015-09-11 | 2017-03-16 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
| EP4269616A3 (en) | 2016-05-02 | 2024-02-14 | Becton, Dickinson and Company | Accurate molecular barcoding |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| IL265478B2 (en) | 2016-09-26 | 2024-10-01 | Becton Dickinson Co | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| US11608497B2 (en) | 2016-11-08 | 2023-03-21 | Becton, Dickinson And Company | Methods for cell label classification |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| EP3601606A2 (en) * | 2017-03-24 | 2020-02-05 | Cellular Research, Inc. | Synthetic multiplets for multiplets determination |
| AU2018281745B2 (en) | 2017-06-05 | 2022-05-19 | Becton, Dickinson And Company | Sample indexing for single cells |
| CN111492068B (zh) | 2017-12-19 | 2025-03-21 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
| WO2019195166A1 (en) * | 2018-04-06 | 2019-10-10 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| ES3014208T3 (en) | 2018-05-03 | 2025-04-21 | Becton Dickinson Co | Molecular barcoding on opposite transcript ends |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
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| WO2017053905A1 (en) * | 2015-09-24 | 2017-03-30 | Abvitro Llc | Affinity-oligonucleotide conjugates and uses thereof |
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| US12084712B2 (en) | 2024-09-10 |
| AU2018281745B2 (en) | 2022-05-19 |
| KR20220124280A (ko) | 2022-09-13 |
| AU2018281745A1 (en) | 2019-10-24 |
| CA3059559A1 (en) | 2018-12-13 |
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| EP4345172A3 (en) | 2024-07-03 |
| US20180346970A1 (en) | 2018-12-06 |
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| US12371729B2 (en) | 2025-07-29 |
| EP3635135A1 (en) | 2020-04-15 |
| AU2022211826B2 (en) | 2025-04-24 |
| JP2025016440A (ja) | 2025-02-04 |
| AU2022211826A1 (en) | 2022-08-25 |
| CN110719959B (zh) | 2021-08-06 |
| US20200172957A1 (en) | 2020-06-04 |
| US20200385780A1 (en) | 2020-12-10 |
| WO2018226293A1 (en) | 2018-12-13 |
| JP7561792B2 (ja) | 2024-10-04 |
| JP2020522262A (ja) | 2020-07-30 |
| US20180346969A1 (en) | 2018-12-06 |
| JP2022106832A (ja) | 2022-07-20 |
| US10669570B2 (en) | 2020-06-02 |
| KR102790050B1 (ko) | 2025-04-04 |
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