ATE361996T1 - Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen - Google Patents

Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen

Info

Publication number
ATE361996T1
ATE361996T1 AT02721342T AT02721342T ATE361996T1 AT E361996 T1 ATE361996 T1 AT E361996T1 AT 02721342 T AT02721342 T AT 02721342T AT 02721342 T AT02721342 T AT 02721342T AT E361996 T1 ATE361996 T1 AT E361996T1
Authority
AT
Austria
Prior art keywords
methods
rna
interest
multiple copies
primer
Prior art date
Application number
AT02721342T
Other languages
English (en)
Inventor
Nurith Kurn
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Application granted granted Critical
Publication of ATE361996T1 publication Critical patent/ATE361996T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
AT02721342T 2001-03-09 2002-03-11 Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen ATE361996T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27455001P 2001-03-09 2001-03-09

Publications (1)

Publication Number Publication Date
ATE361996T1 true ATE361996T1 (de) 2007-06-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AT02721342T ATE361996T1 (de) 2001-03-09 2002-03-11 Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen

Country Status (17)

Country Link
US (7) US6946251B2 (de)
EP (1) EP1366197B1 (de)
JP (3) JP4542312B2 (de)
KR (1) KR20030082535A (de)
CN (1) CN1289690C (de)
AT (1) ATE361996T1 (de)
AU (1) AU2002252279B2 (de)
BR (1) BR0205268A (de)
CA (1) CA2416963C (de)
DE (1) DE60220025T2 (de)
HK (1) HK1059097A1 (de)
IL (1) IL153504A0 (de)
MX (1) MXPA02012739A (de)
NO (1) NO20030557D0 (de)
NZ (1) NZ523167A (de)
WO (1) WO2002072772A2 (de)
ZA (1) ZA200210369B (de)

Families Citing this family (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2002000938A2 (en) 2000-06-26 2002-01-03 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU2002228974A1 (en) 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7094536B2 (en) * 2001-03-09 2006-08-22 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
ATE397095T1 (de) 2001-04-20 2008-06-15 Penn State Res Found Verfahren zur manipulation von nukleinsäuren
AU2003222269A1 (en) * 2002-03-11 2003-09-29 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
CA2480525A1 (en) * 2002-03-29 2003-10-09 Nugen Technologies, Inc. Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification
CA2486283A1 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
AU2003294447A1 (en) * 2002-11-21 2004-06-18 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
EP2374900B1 (de) 2003-03-07 2016-07-13 Rubicon Genomics, Inc. Polynukleotide zur amplifikation und analyse von gesamtgenom- und gesamttranskriptom-bibliotheken, die durch ein dns-polymerisierungsverfahren erzeugt wurden
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US7402386B2 (en) * 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US20080026950A1 (en) * 2004-02-24 2008-01-31 Mitsubishi Rayon Co., Ltd. Gene Relating to Estimation of Postoperative Prognosis for Breast Cancer
WO2005090607A1 (en) 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
WO2005102281A2 (en) * 2004-04-16 2005-11-03 University Of Massachusetts Porous calcium phosphate networks for synthetic bone material
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
AU2005280162B2 (en) * 2004-08-27 2012-04-26 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
WO2006033487A1 (en) * 2004-09-21 2006-03-30 Genomictree Inc. Method for linear amplification of rna using high-heel primer
WO2006086668A2 (en) * 2005-02-09 2006-08-17 Epicentre Technologies Compositions and methods employing 5'-phosphate-dependent nucleic acid exonucleases
US7888125B2 (en) 2005-05-09 2011-02-15 Theranos, Inc. Calibration of fluidic devices
EP1915618A4 (de) 2005-06-02 2009-09-30 Fluidigm Corp Auswertung mit mikrofluiden aufteilungsvorrichtungen
EP1924705A1 (de) 2005-08-02 2008-05-28 Rubicon Genomics, Inc. Isolierung von cpg-inseln durch thermische segregation und enzymatisches selektions-amplifikations-verfahren
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
US20070048741A1 (en) * 2005-08-24 2007-03-01 Getts Robert C Methods and kits for sense RNA synthesis
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8979770B2 (en) * 2006-02-24 2015-03-17 Merck Sharp & Dohme Corp. Extraction and diagnostic fluid devices, systems and methods of use
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US20090275486A1 (en) * 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2038429B1 (de) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Verfahren zur fragmentierung und etikettierung von nukleinsäuren
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
US20090047673A1 (en) 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
CA2934220C (en) 2007-10-02 2019-11-05 Theranos, Inc. Modular point-of-care devices and uses thereof
JP2011500092A (ja) * 2007-10-26 2011-01-06 ロゼッタ、インファーマティクス、リミテッド、ライアビリティ、カンパニー 非ランダムプライマーを用いたcDNA合成の方法
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009102896A2 (en) * 2008-02-12 2009-08-20 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
WO2009117698A2 (en) * 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US20110118125A1 (en) * 2008-05-03 2011-05-19 Tufts Medical Center, Inc. Neonatal salivary genomics
JP2011522521A (ja) 2008-05-05 2011-08-04 ロスアラモス ナショナル セキュリティ,エルエルシー 高度に単純化された側方流動ベースの核酸サンプル調製および受動的流体流動制御
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
CA2773887A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
JPWO2011055737A1 (ja) * 2009-11-06 2013-03-28 株式会社ニッポンジーン 耐熱性鎖置換型dnaポリメラーゼ及び該dnaポリメラーゼの生産方法
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
CN103370425B (zh) * 2010-12-17 2019-03-19 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CN106248582B (zh) 2011-01-21 2020-10-20 拉布拉多诊断有限责任公司 样品使用最大化的系统和方法
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
KR101508670B1 (ko) 2011-04-20 2015-04-07 메사 테크 인터내셔널, 인코포레이티드 핵산 검출 및 동정을 위한 통합 장치
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
TWI624543B (zh) 2011-09-25 2018-05-21 賽瑞諾斯有限公司 用於多重分析的系統和方法
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
GB201121869D0 (en) * 2011-11-17 2012-02-01 Clarient Inc Method of allele-specific amplification
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
EP3578697B1 (de) 2012-01-26 2024-03-06 Tecan Genomics, Inc. Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und hocheffizienten erzeugung einer bibliothek
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
EP4026912A1 (de) 2012-05-10 2022-07-13 The General Hospital Corporation Verfahren zur bestimmung einer nukleotidsequenz
KR101184566B1 (ko) 2012-05-11 2012-09-20 케이맥(주) 실시간 중합효소 연쇄반응과 dna 칩이 통합된 검사 시스템 및 이를 이용한 통합 분석방법
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140081665A1 (en) 2012-09-11 2014-03-20 Theranos, Inc. Information management systems and methods using a biological signature
CN102962015B (zh) * 2012-11-20 2014-04-23 北京大学 以固定的纳米材料微球作基底的dna或rna合成装置
AU2014216060A1 (en) 2013-02-18 2015-08-20 Theranos Ip Company, Llc Systems and methods for collecting and transmitting assay results
US9745571B2 (en) * 2013-03-07 2017-08-29 Bio-Rad Laboratories, Inc. Repetitive reverse transcription partition assay
US9428747B2 (en) * 2013-03-15 2016-08-30 Lyle J. Arnold Methods for amplification of nucleic acids utilizing hairpin loop or duplex primers
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
US9907867B2 (en) 2013-09-26 2018-03-06 General Electric Company Systems, methods and apparatus for manufacturing radioisotopes
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
SG11201604737VA (en) 2013-12-11 2016-07-28 Accuragen Inc Compositions and methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US10450597B2 (en) 2014-01-27 2019-10-22 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US10612073B2 (en) * 2015-02-26 2020-04-07 Hitachi High-Technologies Corporation Method for constructing nucleic acid molecule
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
CN104673786A (zh) * 2015-03-09 2015-06-03 武汉格蓝丽富科技有限公司 一种选择性扩增rna的方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
MX2018004284A (es) 2015-10-09 2018-05-16 Accuragen Holdings Ltd Metodos y composiciones para el enriquecimiento de productos de amplificacion.
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
WO2017201102A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN107541508A (zh) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒
SG10202111825YA (en) 2016-08-15 2021-12-30 Accuragen Holdings Ltd Compositions and methods for detecting rare sequence variants
EP4353830A2 (de) 2016-09-15 2024-04-17 ArcherDX, LLC Verfahren zur herstellung von nukleinsäureproben zur analyse von zellfreier dna
US10704082B2 (en) 2016-09-15 2020-07-07 ArcherDX, Inc. Methods of nucleic acid sample preparation
EP3516400B1 (de) 2016-09-26 2023-08-16 Becton, Dickinson and Company Messung der proteinexpression mit reagenzien mit strichcodierten oligonukleotidsequenzen
CN109923203A (zh) 2016-09-30 2019-06-21 多伦多大学管理委员会 用于鉴定和靶向异质群体中的个体细胞以选择性提取细胞内容物的系统
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたdnaライブラリーの作製方法
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
CN107287320A (zh) * 2017-07-12 2017-10-24 曹国君 Gapdh基因的lamp检测用引物组合及试剂盒
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US11773441B2 (en) 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170A1 (de) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molekulare barcodierung auf gegenüberliegenden transkriptenden
KR20210016560A (ko) 2018-06-06 2021-02-16 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
JP2022506546A (ja) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
EP3894552A1 (de) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
CN113924041A (zh) 2019-03-14 2022-01-11 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
CN113785074A (zh) * 2019-03-25 2021-12-10 斯坦福大学托管董事会 用于诊断细菌感染与病毒感染的特征
KR102249111B1 (ko) * 2019-04-22 2021-05-07 포항공과대학교 산학협력단 신규한 등온 단일 반응용 프로브 세트 및 이의 용도
CN110241178B (zh) * 2019-06-25 2023-03-31 北京博奥晶方生物科技有限公司 一种单细胞转录组测序高通量快速文库制备方法及检测试剂盒
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
JP2023500679A (ja) 2019-11-08 2023-01-10 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
EP4150118A1 (de) 2020-05-14 2023-03-22 Becton Dickinson and Company Primer zur profilierung von immunrepertoire
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN114410778B (zh) * 2021-12-29 2024-03-19 中南大学湘雅医院 Pf543在制备pd-l1/pd-1单抗肿瘤免疫治疗药物中的应用

Family Cites Families (246)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4174384A (en) 1975-06-30 1979-11-13 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US3999345A (en) 1975-11-10 1976-12-28 Shatterproof Glass Corporation Spandrel units
US4261968A (en) * 1979-05-10 1981-04-14 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
JPS57501692A (de) 1980-09-24 1982-09-16
US4362867A (en) 1980-12-10 1982-12-07 Research Corporation Recombinant cDNA construction method and hybrid nucleotides useful in cloning
ATE53862T1 (de) 1982-01-22 1990-06-15 Cetus Corp Verfahren zur charakterisierung von hla und die darin benutzten cdns-testmittel.
US4582788A (en) 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5721098A (en) 1986-01-16 1998-02-24 The Regents Of The University Of California Comparative genomic hybridization
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US5849478A (en) 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
DE3634356A1 (de) 1986-10-08 1988-04-21 Epis Sa Arzneimittel enthaltend alpha-halogenierte dicarbonsaeuren
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
JP2774121B2 (ja) 1987-07-31 1998-07-09 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 標的ポリヌクレオチド配列の選択的増幅
US6090591A (en) 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
US6004745A (en) 1987-09-21 1999-12-21 Gen-Probe Incorporated Hybridization protection assay
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
DE68908054T2 (de) 1988-01-21 1994-03-10 Genentech Inc Verstärkung und nachweis von nukleinsäuresequenzen.
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
WO1989009284A1 (en) 1988-03-24 1989-10-05 University Of Iowa Research Foundation Catalytic hybridization systems for the detection of nucleic acid sequences based on their activity as cofactors in catalytic reactions in which a complementary labeled nucleic acid probe is cleaved
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5708154A (en) * 1989-02-24 1998-01-13 City Of Hope RNA-DNA hybrid molecules of nucleic acid
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5766849A (en) 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
DK0408295T3 (da) 1989-07-11 1996-09-16 Gen Probe Inc Fremgangsmåder til opformering af nukleinsyresekvenser
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
CA2037349C (en) 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
HU218095B (hu) 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5427911A (en) 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
US5194370A (en) 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5527872A (en) 1990-09-14 1996-06-18 At&T Global Information Solutions Company Electronic device with a spin-on glass dielectric layer
US6083689A (en) * 1990-10-16 2000-07-04 Bayer Corporation Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5175243A (en) 1990-11-14 1992-12-29 Phillips Petroleum Company Process for preparing arylene sulfide polymers with halo benzene containing deactivating group
AU8997991A (en) 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
KR930009227B1 (ko) 1991-01-31 1993-09-24 삼성전자 주식회사 테이프 레코더의 브레이크 구동장치
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5090591A (en) * 1991-03-18 1992-02-25 Longford Equipment International Limited Article dispenser for use with continuous strip of articles
WO1992018521A1 (en) 1991-04-10 1992-10-29 Life Technologies, Inc. Method for amplifying and altering an rna sequence
US5340716A (en) 1991-06-20 1994-08-23 Snytex (U.S.A.) Inc. Assay method utilizing photoactivated chemiluminescent label
US5169766A (en) 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5328985A (en) * 1991-07-12 1994-07-12 The Regents Of The University Of California Recombinant streptavidin-protein chimeras useful for conjugation of molecules in the immune system
US5665539A (en) 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
ATE272068T1 (de) 1991-11-07 2004-08-15 Nanotronics Inc Hybridisierung von mit chromophoren und fluorophoren konjugierten polynukleotiden zur erzeugung eines donor-donor energietransfersystems
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
CA2129444A1 (en) 1992-02-04 1993-08-05 Richard C. Ebersole Amplification of assay reporters by nucleic acid replication
ATE231920T1 (de) 1992-03-11 2003-02-15 Dana Farber Cancer Inst Inc Methode um mrna zu klonieren
US5262311A (en) 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA
WO1993022461A1 (en) 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
EP1262560A2 (de) 1992-05-29 2002-12-04 Abbott Laboratories Methode zur Herstellung von cDNA ausgehend von einer RNA Zielsequenz in einer Probe
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
US5766846A (en) * 1992-07-10 1998-06-16 Athena Neurosciences Methods of screening for compounds which inhibit soluble β-amyloid peptide production
JPH07509365A (ja) 1992-07-31 1995-10-19 デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング ポリヌクレオチド類の3’末端に特定配列を導入する方法
WO1994003812A1 (en) * 1992-07-31 1994-02-17 Syntex (U.S.A.) Inc. Photoactivatable chemiluminescent matrices
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5445935A (en) 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
US5571669A (en) 1993-01-14 1996-11-05 The University Of Pennsylvania Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
US5985548A (en) 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5591575A (en) 1993-04-07 1997-01-07 Amersham International Plc Subtraction hybridization employing aziridinylbenoquinone cross-linking agents
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6096715A (en) 1993-05-07 2000-08-01 City Of Hope Chimeric DNA-RNA catalytic sequences
JPH06327500A (ja) * 1993-05-20 1994-11-29 Toyobo Co Ltd 核酸配列の増幅方法および検出方法
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
JPH0723799A (ja) 1993-07-13 1995-01-27 Hitachi Ltd ポリヌクレオチドの検出方法
US6027923A (en) 1993-07-23 2000-02-22 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
CA2169837A1 (en) 1993-08-18 1995-02-23 Peter D. Duck Compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
EP0765401B1 (de) 1993-11-17 2001-02-21 Amersham Pharmacia Biotech UK Limited Verfahren zur massenspektroskopischen sequenzanalyse einer nukleinsäure mittels primerverlängerung
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
JP3175110B2 (ja) 1994-02-07 2001-06-11 オーキッド・バイオサイエンシーズ・インコーポレイテッド リガーゼ/ポリメラーゼ媒体された単一ヌクレオチド多型のジェネティックビットアナリシスおよび遺伝子解析におけるその使用
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5705332A (en) 1994-04-25 1998-01-06 University Of Hawaii Detection and identification of Salmonella and Shigella
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
FR2724934B1 (fr) 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US6280935B1 (en) 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US6309843B1 (en) 1994-10-25 2001-10-30 The Curators Of The University Of Missouri Glycoprotein for use in determining endometrial receptivity
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5932450A (en) 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5763178A (en) 1995-06-07 1998-06-09 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US5916777A (en) 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
JPH0920263A (ja) 1995-07-06 1997-01-21 Jidosha Kiki Co Ltd 電動ポンプ式動力舵取装置
US5989813A (en) 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
WO1997004123A1 (en) 1995-07-19 1997-02-06 Gel Tech Group Inc. Collagen compound production in plants
FR2737223B1 (fr) 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US5652356A (en) 1995-08-17 1997-07-29 Hybridon, Inc. Inverted chimeric and hybrid oligonucleotides
US6068829A (en) 1995-09-11 2000-05-30 The Burnham Institute Method of identifying molecules that home to a selected organ in vivo
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5747255A (en) 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AU714486B2 (en) 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
WO1997021821A1 (en) 1995-12-15 1997-06-19 Amersham Life Science, Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5932449A (en) 1996-02-01 1999-08-03 The United States Of America As Represented By The Secretary Of The Army Detection of botulinum toxin
GB9604267D0 (en) 1996-02-29 1996-05-01 Royal Infirmary Of Edinburgh N Mutation assay
US5712127A (en) * 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
EP1736554B1 (de) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion
WO1998001050A1 (en) 1996-07-08 1998-01-15 Mardix Bar Cochva Custom insoles
US6218105B1 (en) * 1996-07-19 2001-04-17 Kathleen S. Hall High throughput papilloma virus in vitro infectivity assay
US5858665A (en) 1996-07-25 1999-01-12 Navix, Inc. Homogeneous diagnostic assay method utilizing simultaneous target and signal amplification
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
WO1998006700A1 (en) 1996-08-09 1998-02-19 Merck & Co., Inc. Stereoselective deoxygenation reaction
WO1998006736A1 (en) 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
US6255060B1 (en) * 1996-11-21 2001-07-03 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
DE19653439A1 (de) 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
ATE545710T1 (de) 1997-04-01 2012-03-15 Illumina Cambridge Ltd Verfahren zur vervielfältigung von nukleinsäuren
DE69836587T2 (de) 1997-04-16 2007-10-11 Applera Corp., Foster City Nukleinsäuresammlung
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
JP2002508664A (ja) 1997-06-25 2002-03-19 オーキッド・バイオサイエンシーズ・インコーポレイテッド 複数の単一ヌクレオチド多型を単一の反応で検出する方法
US6136533A (en) 1997-07-03 2000-10-24 Id Biomedical Additives for use in cycling probe reactions
US6140086A (en) 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
DK1032705T3 (da) 1997-10-30 2012-03-26 Cold Spring Harbor Lab Probearrays og fremgangsmåder til at benytte probearrays til at skelne mellem DNA
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6345481B1 (en) 1997-11-25 2002-02-12 Premark Rwp Holdings, Inc. Article with interlocking edges and covering product prepared therefrom
WO1999029901A1 (en) 1997-12-11 1999-06-17 The General Hospital Corporation Broad range pcr amplification techniques
JP4317953B2 (ja) 1998-01-22 2009-08-19 独立行政法人理化学研究所 Dnaの塩基配列決定方法
US20010000077A1 (en) 1998-02-03 2001-03-29 Engelhardt Dean L. Novel process, construct and conjugate for producing multiple nucleic acid copies
WO1999040219A1 (en) 1998-02-05 1999-08-12 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6087103A (en) * 1998-03-04 2000-07-11 Lifespan Biosciences, Inc. Tagged ligand arrays for identifying target-ligand interactions
DE19813317A1 (de) * 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
WO1999058724A1 (en) * 1998-05-08 1999-11-18 Life Technologies, Inc. A method for synthesizing a nucleic acid molecule using a ribonuclease
US6297170B1 (en) * 1998-06-23 2001-10-02 Vlsi Technology, Inc. Sacrificial multilayer anti-reflective coating for mos gate formation
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
GB9817055D0 (en) 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
CA2245039A1 (en) 1998-08-13 2000-02-13 Vito Scalia Primer-specific and mispair extension assay for identifying gene variation
DE69937223T3 (de) * 1998-11-09 2011-05-05 Eiken Kagaku K.K. Verfahren zur synthese von nukleinsäuren
US20030049657A1 (en) 1998-11-13 2003-03-13 Cherry Joshua L. Use of primers containing non-replicatable residues for improved cycle-sequencing of nucleic acids
US6927024B2 (en) 1998-11-30 2005-08-09 Genentech, Inc. PCR assay
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
CA2360011A1 (en) 1999-01-05 2000-07-13 Trustees Of Boston University Ordered gene assembly
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
US6951722B2 (en) 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
AU3519900A (en) 1999-03-19 2000-10-09 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
ATE362532T1 (de) * 1999-03-19 2007-06-15 Takara Bio Inc Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US6132997A (en) 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
DK1218542T3 (da) 1999-09-13 2004-08-02 Nugen Technologies Inc Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6107061A (en) 1999-09-18 2000-08-22 The Perkin-Elmer Corporation Modified primer extension reactions for polynucleotide sequence detection
US6617442B1 (en) 1999-09-30 2003-09-09 Isis Pharmaceuticals, Inc. Human Rnase H1 and oligonucleotide compositions thereof
US6300073B1 (en) 1999-10-01 2001-10-09 Clontech Laboratories, Inc. One step RT-PCR methods, enzyme mixes and kits for use in practicing the same
US6271002B1 (en) 1999-10-04 2001-08-07 Rosetta Inpharmatics, Inc. RNA amplification method
US6794138B1 (en) 1999-12-16 2004-09-21 Affymetrix, Inc. Methods of small sample amplification
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
US6376191B1 (en) 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
WO2001073134A2 (en) 2000-03-28 2001-10-04 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Gene profiling arrays
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002000938A2 (en) * 2000-06-26 2002-01-03 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6379932B1 (en) 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
WO2002016639A1 (fr) 2000-08-23 2002-02-28 Takara Bio Inc. Procede d'amplification d'acide nucleique
CA2356123A1 (en) 2000-08-25 2002-02-25 Riken Method of preparing normalized and/or subtracted cdna
WO2002028876A2 (en) 2000-10-05 2002-04-11 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.
US6815164B2 (en) 2000-10-06 2004-11-09 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US6673549B1 (en) * 2000-10-12 2004-01-06 Incyte Corporation Genes expressed in C3A liver cell cultures treated with steroids
US20020127575A1 (en) 2000-10-30 2002-09-12 Glenn Hoke Partially double-stranded nucleic acids, methods of making, and use thereof
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
AU2002228974A1 (en) * 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
GB0101397D0 (en) 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US7094536B2 (en) * 2001-03-09 2006-08-22 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
EP1416047A4 (de) 2001-07-09 2007-03-14 Asahi Chemical Ind Verfahren zur reinigung von nukleinsäuren mit einem vliesstoff und nachweisverfahren
EP1281757A1 (de) 2001-07-31 2003-02-05 Direvo Biotech AG Methode zur Herstellung von Nukleinsäuren mit stochastisch kombinierten Abschnitten von Ausgangsnukleinsäuren
GB0118758D0 (en) 2001-08-01 2001-09-26 Sybesma Wilbert F H Chimeric primed based real time rt-pcr for qualification of rna or dna templates in a crude sample
EP1420069A4 (de) 2001-08-20 2005-11-02 Takara Bio Inc Nukleinsäureamplifikationsverfahren
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
AU2002352858A1 (en) 2001-11-20 2003-06-10 Exact Sciences Corporation Automated sample preparation methods and devices
AU2003222269A1 (en) * 2002-03-11 2003-09-29 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
US20060246434A1 (en) 2002-03-15 2006-11-02 Erlander Mark G Nucleic acid amplification
CA2480525A1 (en) 2002-03-29 2003-10-09 Nugen Technologies, Inc. Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification
CA2486283A1 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
US7205128B2 (en) * 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
DE602004024034D1 (de) * 2003-01-29 2009-12-24 454 Corp Nukleinsäureamplifikation auf basis von kügelchenemulsion
US20060183132A1 (en) 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20040241732A1 (en) 2003-04-11 2004-12-02 Chu-An Chang Method of generating long nucleic acid molecules of defined sequence
US7402386B2 (en) * 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
GB0318110D0 (en) 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP1866434B1 (de) 2005-02-19 2013-06-05 Avacta Group plc Isotherme nukleinsäureamplifikation
WO2006099579A2 (en) 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
CA2621146A1 (en) 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US20090275486A1 (en) 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2038429B1 (de) 2006-06-30 2013-08-21 Nugen Technologies, Inc. Verfahren zur fragmentierung und etikettierung von nukleinsäuren
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US20110224105A1 (en) 2009-08-12 2011-09-15 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
CA2773887A1 (en) 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
GB2487341A (en) 2009-11-02 2012-07-18 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence selection and amplification
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
EP3578697B1 (de) 2012-01-26 2024-03-06 Tecan Genomics, Inc. Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und hocheffizienten erzeugung einer bibliothek

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US9181582B2 (en) 2015-11-10
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