DE60212175D1 - Verfahren zur reduzierung unspezifischer amplifikation bei pcr - Google Patents

Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Info

Publication number
DE60212175D1
DE60212175D1 DE60212175T DE60212175T DE60212175D1 DE 60212175 D1 DE60212175 D1 DE 60212175D1 DE 60212175 T DE60212175 T DE 60212175T DE 60212175 T DE60212175 T DE 60212175T DE 60212175 D1 DE60212175 D1 DE 60212175D1
Authority
DE
Germany
Prior art keywords
sorbitol
specific amplification
amplification
dmso
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60212175T
Other languages
English (en)
Other versions
DE60212175T2 (de
Inventor
J Mclaughlin
Rao Coticone
Will Bloch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of DE60212175D1 publication Critical patent/DE60212175D1/de
Publication of DE60212175T2 publication Critical patent/DE60212175T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Amplifiers (AREA)
DE60212175T 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr Expired - Lifetime DE60212175T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US998887 2001-10-31
US09/998,887 US6783940B2 (en) 2001-10-31 2001-10-31 Method of reducing non-specific amplification in PCR
PCT/US2002/024036 WO2003054209A2 (en) 2001-10-31 2002-07-30 A method of reducing non-specific amplification in pcr

Publications (2)

Publication Number Publication Date
DE60212175D1 true DE60212175D1 (de) 2006-07-20
DE60212175T2 DE60212175T2 (de) 2006-10-12

Family

ID=25545644

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60212175T Expired - Lifetime DE60212175T2 (de) 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Country Status (8)

Country Link
US (1) US6783940B2 (de)
EP (1) EP1446508B1 (de)
JP (1) JP2005512572A (de)
AT (1) ATE329061T1 (de)
AU (1) AU2002365068A1 (de)
CA (1) CA2464885A1 (de)
DE (1) DE60212175T2 (de)
WO (1) WO2003054209A2 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070298423A1 (en) * 2000-03-24 2007-12-27 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by specific amplification and detection of their nucleotide sequences on arrays
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US6841349B2 (en) 2001-05-07 2005-01-11 Applera Corporation Applied Biosystems Group Methods for the reduction of stutter in microsatellite amplification
US7354742B2 (en) * 2002-02-22 2008-04-08 Ortho-Mcneil Pharmaceutical, Inc. Method for generating amplified RNA
EP1403385A1 (de) * 2002-09-27 2004-03-31 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methode zur Darstellung der Replikation von HBV und zum Testen der Sensibilität von Wirkstoffen
CA2543033A1 (en) * 2003-10-16 2005-04-28 Third Wave Technologies, Inc. Direct nucleic acid detection in bodily fluids
EP2042612B1 (de) 2004-01-23 2010-12-15 bioMerieux, Inc. Neuartiges Nukleotidgemisch für eine verbesserte Nukleinsäurenamplifikationsleistung
EP1725680B1 (de) * 2004-02-04 2009-12-23 QIAGEN North American Holdings, Inc. Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation
EP1766061B1 (de) * 2004-05-20 2013-07-17 Quest Diagnostics Investments Incorporated Vergleichende einfachmarkierung-hybridisierung
JP2008518621A (ja) * 2004-11-03 2008-06-05 サード・ウェーブ・テクノロジーズ・インク 一段階検出アッセイ法
JP2006262777A (ja) * 2005-03-24 2006-10-05 Japan Science & Technology Agency 夾雑物を含む試料を用いた核酸合成方法及び添加剤
US20060263811A1 (en) * 2005-05-03 2006-11-23 Geunsook Jeon Materials and kits for use in hot-start PCR, and methods of amplifying nucleic acids in a polymerase chain reaction
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas
US8097414B2 (en) 2005-11-25 2012-01-17 K. K. Dnaform Method for detecting and amplifying nucleic acid
EP2019687B1 (de) 2006-03-29 2014-03-19 Merial Limited Impfstoff gegen streptokokken
US7772390B1 (en) 2006-07-18 2010-08-10 The Regents Of The University Of California Lipid mediated nucleic acid synthesis
KR100773561B1 (ko) * 2006-11-07 2007-11-05 삼성전자주식회사 다중 ｐｃｒ에서 비특이적 증폭을 감소시키는 장치 및 방법
US7507539B2 (en) * 2007-07-30 2009-03-24 Quest Diagnostics Investments Incorporated Substractive single label comparative hybridization
AU2009225835B2 (en) 2008-03-15 2013-02-14 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
EP2285982B1 (de) 2008-05-27 2018-04-11 Dako Denmark A/S Zusammensetzungen und verfahren zum nachweis chromosomaler anomalien mit neuartigen hybridisierungspuffern
JP2012516155A (ja) * 2009-01-27 2012-07-19 キアゲン ゲーザーズバーグ エンドポイント均一蛍光検出を用いた好熱性ヘリカーゼ依存性増幅技術
WO2010097655A1 (en) 2009-02-26 2010-09-02 Dako Denmark A/S Compositions and methods for rna hybridization applications
US10662465B2 (en) 2011-09-30 2020-05-26 Agilent Technologies, Inc. Hybridization compositions and methods using formamide
EP2768974B1 (de) 2011-10-21 2017-07-19 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren
TWI616535B (zh) * 2012-12-18 2018-03-01 梅里亞有限公司 Ｉｂｖ、ｃｓｆｖ及ｎｄｖ之高解析度熔解基因型比對
US9630182B2 (en) * 2013-12-04 2017-04-25 Leidos Innovations Technology, Inc. Non-contact infrared thermocycling
WO2019028430A1 (en) * 2017-08-03 2019-02-07 The Trustees Of Columbia University In The City Of New York OPTICAL SUPER-MULTIPLEXING BY POLYYNESES

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US5571674A (en) * 1989-09-18 1996-11-05 The Trustees Of Columbia University In The City Of New York DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers
US5606045A (en) * 1990-05-15 1997-02-25 Diatron Corporation Nucleic acid probes and methods
GB9024005D0 (en) * 1990-11-05 1990-12-19 British Bio Technology Process for amplifying nucleic acid
US5364759B2 (en) 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
DE4411588C1 (de) 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5545539A (en) 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6156512A (en) 1996-05-23 2000-12-05 Promega Corp Allelic ladders for short tandem repeat loci
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法

Also Published As

Publication number Publication date
EP1446508B1 (de) 2006-06-07
WO2003054209A3 (en) 2003-12-04
US6783940B2 (en) 2004-08-31
DE60212175T2 (de) 2006-10-12
US20030104395A1 (en) 2003-06-05
ATE329061T1 (de) 2006-06-15
EP1446508A4 (de) 2005-03-09
CA2464885A1 (en) 2003-07-03
WO2003054209A2 (en) 2003-07-03
AU2002365068A1 (en) 2003-07-09
JP2005512572A (ja) 2005-05-12
EP1446508A2 (de) 2004-08-18

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Legal Events

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

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8380 Miscellaneous part iii

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