ATE329061T1 - Verfahren zur reduzierung unspezifischer amplifikation bei pcr - Google Patents

Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Info

Publication number
ATE329061T1
ATE329061T1 AT02802916T AT02802916T ATE329061T1 AT E329061 T1 ATE329061 T1 AT E329061T1 AT 02802916 T AT02802916 T AT 02802916T AT 02802916 T AT02802916 T AT 02802916T AT E329061 T1 ATE329061 T1 AT E329061T1
Authority
AT
Austria
Prior art keywords
sorbitol
specific amplification
dmso
sample
reducing non
Prior art date
Application number
AT02802916T
Other languages
English (en)
Inventor
Ian J Mclaughlin
Sulekha Rao Coticone
Will Bloch
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE329061T1 publication Critical patent/ATE329061T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Amplifiers (AREA)
AT02802916T 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr ATE329061T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/998,887 US6783940B2 (en) 2001-10-31 2001-10-31 Method of reducing non-specific amplification in PCR

Publications (1)

Publication Number Publication Date
ATE329061T1 true ATE329061T1 (de) 2006-06-15

Family

ID=25545644

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02802916T ATE329061T1 (de) 2001-10-31 2002-07-30 Verfahren zur reduzierung unspezifischer amplifikation bei pcr

Country Status (8)

Country Link
US (1) US6783940B2 (de)
EP (1) EP1446508B1 (de)
JP (1) JP2005512572A (de)
AT (1) ATE329061T1 (de)
AU (1) AU2002365068A1 (de)
CA (1) CA2464885A1 (de)
DE (1) DE60212175T2 (de)
WO (1) WO2003054209A2 (de)

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Publication number Priority date Publication date Assignee Title
US20070298423A1 (en) * 2000-03-24 2007-12-27 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by specific amplification and detection of their nucleotide sequences on arrays
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
US6841349B2 (en) 2001-05-07 2005-01-11 Applera Corporation Applied Biosystems Group Methods for the reduction of stutter in microsatellite amplification
US7354742B2 (en) * 2002-02-22 2008-04-08 Ortho-Mcneil Pharmaceutical, Inc. Method for generating amplified RNA
EP1403385A1 (de) * 2002-09-27 2004-03-31 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methode zur Darstellung der Replikation von HBV und zum Testen der Sensibilität von Wirkstoffen
US20050186588A1 (en) * 2003-10-16 2005-08-25 Third Wave Technologies, Inc. Direct nucleic acid detection in bodily fluids
JP4866248B2 (ja) 2004-01-23 2012-02-01 バイオメリュー・インコーポレイテッド 核酸増幅性能改良のための新規ヌクレオチド混合物
WO2005076908A2 (en) * 2004-02-04 2005-08-25 Eppendorf Ag Anti-freeze protein enhanced nucleic acid amplification
CA2567627C (en) * 2004-05-20 2014-09-30 Quest Diagnostics Investments Incorporated Single label comparative hybridization
CA2586246A1 (en) * 2004-11-03 2006-05-11 Third Wave Technologies, Inc. Single step nucleic acid detection assay employing reverse transcription, polymerase chain reaction and endonuclease cleavage
JP2006262777A (ja) * 2005-03-24 2006-10-05 Japan Science & Technology Agency 夾雑物を含む試料を用いた核酸合成方法及び添加剤
US20060263811A1 (en) * 2005-05-03 2006-11-23 Geunsook Jeon Materials and kits for use in hot-start PCR, and methods of amplifying nucleic acids in a polymerase chain reaction
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas
US8097414B2 (en) 2005-11-25 2012-01-17 K. K. Dnaform Method for detecting and amplifying nucleic acid
WO2007115059A2 (en) 2006-03-29 2007-10-11 Merial Limited Vaccine against streptococci
US7772390B1 (en) 2006-07-18 2010-08-10 The Regents Of The University Of California Lipid mediated nucleic acid synthesis
KR100773561B1 (ko) * 2006-11-07 2007-11-05 삼성전자주식회사 다중 ｐｃｒ에서 비특이적 증폭을 감소시키는 장치 및 방법
US7507539B2 (en) * 2007-07-30 2009-03-24 Quest Diagnostics Investments Incorporated Substractive single label comparative hybridization
MX2010010161A (es) 2008-03-15 2011-03-21 Hologic Inc Star Composiciones y metodos para el analisis de moleculas de acido nucleico durante reacciones de amplificacion.
EP3733871A1 (de) 2008-05-27 2020-11-04 Dako Denmark A/S Zusammensetzungen und verfahren zur erkennung von chromosomalen abweichungen mit neuen hybridisierungspuffern
WO2010088273A1 (en) * 2009-01-27 2010-08-05 Qiagen Gaithersburg Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
WO2010097655A1 (en) * 2009-02-26 2010-09-02 Dako Denmark A/S Compositions and methods for rna hybridization applications
US10662465B2 (en) 2011-09-30 2020-05-26 Agilent Technologies, Inc. Hybridization compositions and methods using formamide
EP2768974B1 (de) 2011-10-21 2017-07-19 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren
TWI616535B (zh) * 2012-12-18 2018-03-01 梅里亞有限公司 Ｉｂｖ、ｃｓｆｖ及ｎｄｖ之高解析度熔解基因型比對
US9630182B2 (en) * 2013-12-04 2017-04-25 Leidos Innovations Technology, Inc. Non-contact infrared thermocycling
JP2020532497A (ja) * 2017-08-03 2020-11-12 ザ トラスティーズ オブ コロンビア ユニバーシティ イン ザ シティ オブ ニューヨーク ポリインによる光学的超多重化

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Publication number Priority date Publication date Assignee Title
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US5571674A (en) * 1989-09-18 1996-11-05 The Trustees Of Columbia University In The City Of New York DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers
US5606045A (en) * 1990-05-15 1997-02-25 Diatron Corporation Nucleic acid probes and methods
GB9024005D0 (en) * 1990-11-05 1990-12-19 British Bio Technology Process for amplifying nucleic acid
US5364759B2 (en) 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
DE4411588C1 (de) 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5545539A (en) 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6156512A (en) 1996-05-23 2000-12-05 Promega Corp Allelic ladders for short tandem repeat loci
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法

Also Published As

Publication number Publication date
JP2005512572A (ja) 2005-05-12
US20030104395A1 (en) 2003-06-05
CA2464885A1 (en) 2003-07-03
WO2003054209A2 (en) 2003-07-03
DE60212175T2 (de) 2006-10-12
EP1446508B1 (de) 2006-06-07
US6783940B2 (en) 2004-08-31
EP1446508A2 (de) 2004-08-18
DE60212175D1 (de) 2006-07-20
EP1446508A4 (de) 2005-03-09
WO2003054209A3 (en) 2003-12-04
AU2002365068A1 (en) 2003-07-09

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